TY  - JOUR
AU  - Soković Bajić, Svetlana
AU  - Canas, Maria-Alexandra
AU  - Tolinački, Maja
AU  - Badia, Josefa
AU  - Sanchez, Borja
AU  - Golić, Nataša
AU  - Margolles, Abelardo
AU  - Baldoma, Laura
AU  - Ruas-Madiedo, Patricia
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1339
AB  - In recent years the role of extracellular vesicles (EVs) of Gram-positive bacteria in host-microbe cross-talk has become increasingly appreciated, although the knowledge of their biogenesis, release and host-uptake is still limited. The aim of this study was to characterize the EVs released by the dairy isolate Lactiplantibacillus plantarum BGAN8 and to gain an insight into the putative mechanism of EVs uptake by intestinal epithelial cells. The cryo-TEM observation undoubtedly demonstrated the release of EVs (20 to 140 nm) from the surface of BGAN8, with exopolysaccharides seems to be part of EVs surface. The proteomic analysis revealed that the EVs are enriched in enzymes involved in central metabolic pathways, such as glycolysis, and in membrane components with the most abundant proteins belonging to amino acid/peptide ABC transporters. Putative internalization pathways were evaluated in time-course internalization experiments with non-polarized HT29 cells in the presence of inhibitors of endocytic pathways: chlorpromazine and dynasore (inhibitors of clathrin-mediated endocytosis-CME) and filipin III and nystatin (disrupting lipid rafts). For the first time, our results revealed that the internalization was specifically inhibited by dynasore and chlorpromazine but not by filipin III and nystatin implying that one of the entries of L. plantarum vesicles was through CME pathway.
PB  - Nature Portfolio, Berlin
T2  - Scientific Reports
T1  - Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line
SP  - 21829
VL  - 10
DO  - 10.1038/s41598-020-78920-z
ER  - 

@article{
author = "Soković Bajić, Svetlana and Canas, Maria-Alexandra and Tolinački, Maja and Badia, Josefa and Sanchez, Borja and Golić, Nataša and Margolles, Abelardo and Baldoma, Laura and Ruas-Madiedo, Patricia",
year = "2020",
abstract = "In recent years the role of extracellular vesicles (EVs) of Gram-positive bacteria in host-microbe cross-talk has become increasingly appreciated, although the knowledge of their biogenesis, release and host-uptake is still limited. The aim of this study was to characterize the EVs released by the dairy isolate Lactiplantibacillus plantarum BGAN8 and to gain an insight into the putative mechanism of EVs uptake by intestinal epithelial cells. The cryo-TEM observation undoubtedly demonstrated the release of EVs (20 to 140 nm) from the surface of BGAN8, with exopolysaccharides seems to be part of EVs surface. The proteomic analysis revealed that the EVs are enriched in enzymes involved in central metabolic pathways, such as glycolysis, and in membrane components with the most abundant proteins belonging to amino acid/peptide ABC transporters. Putative internalization pathways were evaluated in time-course internalization experiments with non-polarized HT29 cells in the presence of inhibitors of endocytic pathways: chlorpromazine and dynasore (inhibitors of clathrin-mediated endocytosis-CME) and filipin III and nystatin (disrupting lipid rafts). For the first time, our results revealed that the internalization was specifically inhibited by dynasore and chlorpromazine but not by filipin III and nystatin implying that one of the entries of L. plantarum vesicles was through CME pathway.",
publisher = "Nature Portfolio, Berlin",
journal = "Scientific Reports",
title = "Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line",
pages = "21829",
volume = "10",
doi = "10.1038/s41598-020-78920-z"
}

Soković Bajić, S., Canas, M., Tolinački, M., Badia, J., Sanchez, B., Golić, N., Margolles, A., Baldoma, L.,& Ruas-Madiedo, P.. (2020). Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line. in Scientific Reports
Nature Portfolio, Berlin., 10, 21829.
https://doi.org/10.1038/s41598-020-78920-z

Soković Bajić S, Canas M, Tolinački M, Badia J, Sanchez B, Golić N, Margolles A, Baldoma L, Ruas-Madiedo P. Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line. in Scientific Reports. 2020;10:21829.
doi:10.1038/s41598-020-78920-z .

Soković Bajić, Svetlana, Canas, Maria-Alexandra, Tolinački, Maja, Badia, Josefa, Sanchez, Borja, Golić, Nataša, Margolles, Abelardo, Baldoma, Laura, Ruas-Madiedo, Patricia, "Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line" in Scientific Reports, 10 (2020):21829,
https://doi.org/10.1038/s41598-020-78920-z . .

TY  - CONF
AU  - Soković Bajić, Svetlana
AU  - Canas, Maria-Alexandra
AU  - Lukić, Jovanka
AU  - Radojević, Dušan
AU  - Badia, Josefa
AU  - Margolles, Abelardo
AU  - Golić, Nataša
AU  - Baldoma, Laura
AU  - Ruas-Madiedo, Patricia
AU  - Tolinački, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1393
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Journal of Clinical Gastroenterology
T1  - Characterization of Microvesicles of Strain Lactobacillus Plantarum BGAN8 and Internalization by HT29 Cells
EP  - S24
SP  - S23
VL  - 54
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1393
ER  - 

@conference{
author = "Soković Bajić, Svetlana and Canas, Maria-Alexandra and Lukić, Jovanka and Radojević, Dušan and Badia, Josefa and Margolles, Abelardo and Golić, Nataša and Baldoma, Laura and Ruas-Madiedo, Patricia and Tolinački, Maja",
year = "2020",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Clinical Gastroenterology",
title = "Characterization of Microvesicles of Strain Lactobacillus Plantarum BGAN8 and Internalization by HT29 Cells",
pages = "S24-S23",
volume = "54",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1393"
}

Soković Bajić, S., Canas, M., Lukić, J., Radojević, D., Badia, J., Margolles, A., Golić, N., Baldoma, L., Ruas-Madiedo, P.,& Tolinački, M.. (2020). Characterization of Microvesicles of Strain Lactobacillus Plantarum BGAN8 and Internalization by HT29 Cells. in Journal of Clinical Gastroenterology
Lippincott Williams & Wilkins, Philadelphia., 54, S23-S24.
https://hdl.handle.net/21.15107/rcub_imagine_1393

Soković Bajić S, Canas M, Lukić J, Radojević D, Badia J, Margolles A, Golić N, Baldoma L, Ruas-Madiedo P, Tolinački M. Characterization of Microvesicles of Strain Lactobacillus Plantarum BGAN8 and Internalization by HT29 Cells. in Journal of Clinical Gastroenterology. 2020;54:S23-S24.
https://hdl.handle.net/21.15107/rcub_imagine_1393 .

Soković Bajić, Svetlana, Canas, Maria-Alexandra, Lukić, Jovanka, Radojević, Dušan, Badia, Josefa, Margolles, Abelardo, Golić, Nataša, Baldoma, Laura, Ruas-Madiedo, Patricia, Tolinački, Maja, "Characterization of Microvesicles of Strain Lactobacillus Plantarum BGAN8 and Internalization by HT29 Cells" in Journal of Clinical Gastroenterology, 54 (2020):S23-S24,
https://hdl.handle.net/21.15107/rcub_imagine_1393 .