TY  - CONF
AU  - Erić, K.
AU  - Rosić, Jovana
AU  - Miladinov, Marko
AU  - Dragičević, Sandra
AU  - Barišić, Goran
AU  - Marković, V.
AU  - Zeljić, Katarina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2081
AB  - Background & objectives: The NEAT1 (Nuclear Paraspeckle Assembly Transcript 1) gene encodes a long non-coding RNA that is deregulated in carcinomas of the gastrointestinal tract. Diagnostic and predictive potential of NEAT1 was investigated in patients with locally
advanced rectal cancer.
Methods: The study group consisted of 19 patients with rectal cancer
treated with neoadjuvant chemoradiotherapy (nCRT). RNA was isolated with TRIzol reagent from samples of rectal cancer and noncancerous tissue before and after nCRT. The relative expression level of
NEAT1 normalised to GAPDH was determined by qRT-PCR method.
Results: Expression of NEAT1 did not difer between rectal cancer and
noncancerous tissue before nCRT (p=0.953) and cancer and noncancerous tissue after nCRT (p=0.210). There was no diference in NEAT1
expression between tumour tissue before and after nCRT (p=0.079).
NEAT1 was signifcantly higher in noncancerous tissue before than
after nCRT (p=0.005). Therapy responders (TRG1, TRG2) and nonresponders (TRG3, TRG4) did not difer in NEAT1 levels in tumour
tissue before (p=0.790) and after nCRT (p=0.352). NEAT1 expression
in rectal cancer tissue before nCRT cannot be used as a biomarker to
distinguish responders from non-responders (AUC=0.559, 95%CI=0-
1, p=0.790). Demographic and clinicopathological characteristics were
not associated with NEAT1 expression in rectal cancer tissue.
Conclusion: The obtained results suggest that the long noncoding
RNA NEAT1 cannot be considered as a biomarker with diagnostic
potential or for predicting response to nCRT in patients with rectal
cancer. Validation of the current results in a larger group of patients
with locally advanced rectal cancer is warranted.
PB  - Springer
C3  - Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September
T1  - Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer
EP  - S307
IS  - Supplement 1
SP  - S307
SP  - E-PS-15-011
VL  - 483
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2081
ER  - 

@conference{
author = "Erić, K. and Rosić, Jovana and Miladinov, Marko and Dragičević, Sandra and Barišić, Goran and Marković, V. and Zeljić, Katarina",
year = "2023",
abstract = "Background & objectives: The NEAT1 (Nuclear Paraspeckle Assembly Transcript 1) gene encodes a long non-coding RNA that is deregulated in carcinomas of the gastrointestinal tract. Diagnostic and predictive potential of NEAT1 was investigated in patients with locally
advanced rectal cancer.
Methods: The study group consisted of 19 patients with rectal cancer
treated with neoadjuvant chemoradiotherapy (nCRT). RNA was isolated with TRIzol reagent from samples of rectal cancer and noncancerous tissue before and after nCRT. The relative expression level of
NEAT1 normalised to GAPDH was determined by qRT-PCR method.
Results: Expression of NEAT1 did not difer between rectal cancer and
noncancerous tissue before nCRT (p=0.953) and cancer and noncancerous tissue after nCRT (p=0.210). There was no diference in NEAT1
expression between tumour tissue before and after nCRT (p=0.079).
NEAT1 was signifcantly higher in noncancerous tissue before than
after nCRT (p=0.005). Therapy responders (TRG1, TRG2) and nonresponders (TRG3, TRG4) did not difer in NEAT1 levels in tumour
tissue before (p=0.790) and after nCRT (p=0.352). NEAT1 expression
in rectal cancer tissue before nCRT cannot be used as a biomarker to
distinguish responders from non-responders (AUC=0.559, 95%CI=0-
1, p=0.790). Demographic and clinicopathological characteristics were
not associated with NEAT1 expression in rectal cancer tissue.
Conclusion: The obtained results suggest that the long noncoding
RNA NEAT1 cannot be considered as a biomarker with diagnostic
potential or for predicting response to nCRT in patients with rectal
cancer. Validation of the current results in a larger group of patients
with locally advanced rectal cancer is warranted.",
publisher = "Springer",
journal = "Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September",
title = "Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer",
pages = "S307-S307-E-PS-15-011",
number = "Supplement 1",
volume = "483",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2081"
}

Erić, K., Rosić, J., Miladinov, M., Dragičević, S., Barišić, G., Marković, V.,& Zeljić, K.. (2023). Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer. in Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September
Springer., 483(Supplement 1), S307-S307.
https://hdl.handle.net/21.15107/rcub_imagine_2081

Erić K, Rosić J, Miladinov M, Dragičević S, Barišić G, Marković V, Zeljić K. Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer. in Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September. 2023;483(Supplement 1):S307-S307.
https://hdl.handle.net/21.15107/rcub_imagine_2081 .

Erić, K., Rosić, Jovana, Miladinov, Marko, Dragičević, Sandra, Barišić, Goran, Marković, V., Zeljić, Katarina, "Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer" in Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September, 483, no. Supplement 1 (2023):S307-S307,
https://hdl.handle.net/21.15107/rcub_imagine_2081 .