TY  - CONF
AU  - Erić, K.
AU  - Rosić, Jovana
AU  - Miladinov, Marko
AU  - Dragičević, Sandra
AU  - Barišić, Goran
AU  - Marković, V.
AU  - Zeljić, Katarina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2081
AB  - Background & objectives: The NEAT1 (Nuclear Paraspeckle Assembly Transcript 1) gene encodes a long non-coding RNA that is deregulated in carcinomas of the gastrointestinal tract. Diagnostic and predictive potential of NEAT1 was investigated in patients with locally
advanced rectal cancer.
Methods: The study group consisted of 19 patients with rectal cancer
treated with neoadjuvant chemoradiotherapy (nCRT). RNA was isolated with TRIzol reagent from samples of rectal cancer and noncancerous tissue before and after nCRT. The relative expression level of
NEAT1 normalised to GAPDH was determined by qRT-PCR method.
Results: Expression of NEAT1 did not difer between rectal cancer and
noncancerous tissue before nCRT (p=0.953) and cancer and noncancerous tissue after nCRT (p=0.210). There was no diference in NEAT1
expression between tumour tissue before and after nCRT (p=0.079).
NEAT1 was signifcantly higher in noncancerous tissue before than
after nCRT (p=0.005). Therapy responders (TRG1, TRG2) and nonresponders (TRG3, TRG4) did not difer in NEAT1 levels in tumour
tissue before (p=0.790) and after nCRT (p=0.352). NEAT1 expression
in rectal cancer tissue before nCRT cannot be used as a biomarker to
distinguish responders from non-responders (AUC=0.559, 95%CI=0-
1, p=0.790). Demographic and clinicopathological characteristics were
not associated with NEAT1 expression in rectal cancer tissue.
Conclusion: The obtained results suggest that the long noncoding
RNA NEAT1 cannot be considered as a biomarker with diagnostic
potential or for predicting response to nCRT in patients with rectal
cancer. Validation of the current results in a larger group of patients
with locally advanced rectal cancer is warranted.
PB  - Springer
C3  - Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September
T1  - Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer
EP  - S307
IS  - Supplement 1
SP  - S307
SP  - E-PS-15-011
VL  - 483
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2081
ER  - 

@conference{
author = "Erić, K. and Rosić, Jovana and Miladinov, Marko and Dragičević, Sandra and Barišić, Goran and Marković, V. and Zeljić, Katarina",
year = "2023",
abstract = "Background & objectives: The NEAT1 (Nuclear Paraspeckle Assembly Transcript 1) gene encodes a long non-coding RNA that is deregulated in carcinomas of the gastrointestinal tract. Diagnostic and predictive potential of NEAT1 was investigated in patients with locally
advanced rectal cancer.
Methods: The study group consisted of 19 patients with rectal cancer
treated with neoadjuvant chemoradiotherapy (nCRT). RNA was isolated with TRIzol reagent from samples of rectal cancer and noncancerous tissue before and after nCRT. The relative expression level of
NEAT1 normalised to GAPDH was determined by qRT-PCR method.
Results: Expression of NEAT1 did not difer between rectal cancer and
noncancerous tissue before nCRT (p=0.953) and cancer and noncancerous tissue after nCRT (p=0.210). There was no diference in NEAT1
expression between tumour tissue before and after nCRT (p=0.079).
NEAT1 was signifcantly higher in noncancerous tissue before than
after nCRT (p=0.005). Therapy responders (TRG1, TRG2) and nonresponders (TRG3, TRG4) did not difer in NEAT1 levels in tumour
tissue before (p=0.790) and after nCRT (p=0.352). NEAT1 expression
in rectal cancer tissue before nCRT cannot be used as a biomarker to
distinguish responders from non-responders (AUC=0.559, 95%CI=0-
1, p=0.790). Demographic and clinicopathological characteristics were
not associated with NEAT1 expression in rectal cancer tissue.
Conclusion: The obtained results suggest that the long noncoding
RNA NEAT1 cannot be considered as a biomarker with diagnostic
potential or for predicting response to nCRT in patients with rectal
cancer. Validation of the current results in a larger group of patients
with locally advanced rectal cancer is warranted.",
publisher = "Springer",
journal = "Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September",
title = "Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer",
pages = "S307-S307-E-PS-15-011",
number = "Supplement 1",
volume = "483",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2081"
}

Erić, K., Rosić, J., Miladinov, M., Dragičević, S., Barišić, G., Marković, V.,& Zeljić, K.. (2023). Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer. in Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September
Springer., 483(Supplement 1), S307-S307.
https://hdl.handle.net/21.15107/rcub_imagine_2081

Erić K, Rosić J, Miladinov M, Dragičević S, Barišić G, Marković V, Zeljić K. Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer. in Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September. 2023;483(Supplement 1):S307-S307.
https://hdl.handle.net/21.15107/rcub_imagine_2081 .

Erić, K., Rosić, Jovana, Miladinov, Marko, Dragičević, Sandra, Barišić, Goran, Marković, V., Zeljić, Katarina, "Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer" in Virchows Archiv, 35th European Congress of Pathology  Pathology – a bridge between Science and Medicine, 9 – 13 September, 483, no. Supplement 1 (2023):S307-S307,
https://hdl.handle.net/21.15107/rcub_imagine_2081 .

TY  - CONF
AU  - Erić, K.
AU  - Miladinov, Marko
AU  - Dragičević, Sandra
AU  - Rosić, Jovana
AU  - Krivokapić, Zoran
AU  - Zeljić, Katarina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2083
AB  - Background & objectives: Long non coding RNA, H19 is an imprinted, maternally expressed gene, usually deregulated in diferent cancer types, including rectal cancer. This study aimed to investigate H19 role as a potential biomarker to predict therapy response in rectal cancer patients. Methods: The study included 14 patients diagnosed with rectal cancer, treated with neoadjuvant chemoradio therapy (nCRT). RNA was isolated by TRIzol reagent from samples of rectal cancer tissue  before and after nCRT. Relative expression of H19 was normal- ized to housekeeping GAPDH gene, and expression was analysed  by quantitative real-time PCR. Relative expression of H19 was calculated by 2-ΔCt method. Results: Relative expression of H19 was significantly increased in rectal cancer tissue after nCRT (0.244±0.408) compared to the tissue before nCRT (0.043±0.055), p=0.004, Wilcoxon test. According to tumour regression grade (TRG), 85.71% (12/14) of  patients did not respond, while 14.28% (2/14) responded to pre- operative CRT. Responders (TRG1, TRG2) and non-responders  (TRG3, TRG4) did not differ in H19 expression in tumour tissue before (p=0.659, Mann-Whitney U test) as well as after nCRT  (p=0.999, Mann-Whitney U test). Receiver operating curve analy- sis indicates that H19 expression in colorectal tissue before nCRT  can not be used as a biomarker for distinguishing responders from non-responders (AUC=0.625, 95%CI=0.257-0.992, p=0.583).  Conclusion: Our study suggests H19 upregulation upon neoadju- vant chemoradiotherapy in rectal cancer. The potential predictive  value of H19 as a biomarker of therapy response should be studied in a larger group of patients.
PB  - Springer
C3  - Virchows Archiv, 34 th European Congress of Pathology - Abstracts
T1  - Long non-coding RNA H19 expression in rectal cancer and therapy response
EP  - S145
IS  - Supplement 1
SP  - S145
SP  - PS-15-017
VL  - 481
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2083
ER  - 

@conference{
author = "Erić, K. and Miladinov, Marko and Dragičević, Sandra and Rosić, Jovana and Krivokapić, Zoran and Zeljić, Katarina",
year = "2022",
abstract = "Background & objectives: Long non coding RNA, H19 is an imprinted, maternally expressed gene, usually deregulated in diferent cancer types, including rectal cancer. This study aimed to investigate H19 role as a potential biomarker to predict therapy response in rectal cancer patients. Methods: The study included 14 patients diagnosed with rectal cancer, treated with neoadjuvant chemoradio therapy (nCRT). RNA was isolated by TRIzol reagent from samples of rectal cancer tissue  before and after nCRT. Relative expression of H19 was normal- ized to housekeeping GAPDH gene, and expression was analysed  by quantitative real-time PCR. Relative expression of H19 was calculated by 2-ΔCt method. Results: Relative expression of H19 was significantly increased in rectal cancer tissue after nCRT (0.244±0.408) compared to the tissue before nCRT (0.043±0.055), p=0.004, Wilcoxon test. According to tumour regression grade (TRG), 85.71% (12/14) of  patients did not respond, while 14.28% (2/14) responded to pre- operative CRT. Responders (TRG1, TRG2) and non-responders  (TRG3, TRG4) did not differ in H19 expression in tumour tissue before (p=0.659, Mann-Whitney U test) as well as after nCRT  (p=0.999, Mann-Whitney U test). Receiver operating curve analy- sis indicates that H19 expression in colorectal tissue before nCRT  can not be used as a biomarker for distinguishing responders from non-responders (AUC=0.625, 95%CI=0.257-0.992, p=0.583).  Conclusion: Our study suggests H19 upregulation upon neoadju- vant chemoradiotherapy in rectal cancer. The potential predictive  value of H19 as a biomarker of therapy response should be studied in a larger group of patients.",
publisher = "Springer",
journal = "Virchows Archiv, 34 th European Congress of Pathology - Abstracts",
title = "Long non-coding RNA H19 expression in rectal cancer and therapy response",
pages = "S145-S145-PS-15-017",
number = "Supplement 1",
volume = "481",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2083"
}

Erić, K., Miladinov, M., Dragičević, S., Rosić, J., Krivokapić, Z.,& Zeljić, K.. (2022). Long non-coding RNA H19 expression in rectal cancer and therapy response. in Virchows Archiv, 34 th European Congress of Pathology - Abstracts
Springer., 481(Supplement 1), S145-S145.
https://hdl.handle.net/21.15107/rcub_imagine_2083

Erić K, Miladinov M, Dragičević S, Rosić J, Krivokapić Z, Zeljić K. Long non-coding RNA H19 expression in rectal cancer and therapy response. in Virchows Archiv, 34 th European Congress of Pathology - Abstracts. 2022;481(Supplement 1):S145-S145.
https://hdl.handle.net/21.15107/rcub_imagine_2083 .

Erić, K., Miladinov, Marko, Dragičević, Sandra, Rosić, Jovana, Krivokapić, Zoran, Zeljić, Katarina, "Long non-coding RNA H19 expression in rectal cancer and therapy response" in Virchows Archiv, 34 th European Congress of Pathology - Abstracts, 481, no. Supplement 1 (2022):S145-S145,
https://hdl.handle.net/21.15107/rcub_imagine_2083 .