TY  - JOUR
AU  - Dworkin, J
AU  - Jovanović, Goran
AU  - Model, P
PY  - 2000
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/143
AB  - In Eubacteria, expression of genes transcribed by an RNA polymerase holoenzyme containing the alternate sigma factor sigma(54) is positively regulated by proteins belonging to the family of enhancer-binding proteins (EBPs), These proteins bind to upstream activation sequences and are required for the initiation of transcription at the sigma(54)-dependent promoters. They are typically inactive until modified in their N-terminal regulatory domain either by specific phosphorylation or by the binding of a small effector molecule. EBPs lacking this domain, such as the PspF activator of the sigma(54)-dependent pspA promoter, are constitutively active. We describe here the in vivo and in vitro properties of the PspA protein of Escherichia coli, which negatively regulates expression of the pspA promoter without binding DNA directly.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription
EP  - 319
IS  - 2
SP  - 311
VL  - 182
DO  - 10.1128/JB.182.2.311-319.2000
ER  - 

@article{
author = "Dworkin, J and Jovanović, Goran and Model, P",
year = "2000",
abstract = "In Eubacteria, expression of genes transcribed by an RNA polymerase holoenzyme containing the alternate sigma factor sigma(54) is positively regulated by proteins belonging to the family of enhancer-binding proteins (EBPs), These proteins bind to upstream activation sequences and are required for the initiation of transcription at the sigma(54)-dependent promoters. They are typically inactive until modified in their N-terminal regulatory domain either by specific phosphorylation or by the binding of a small effector molecule. EBPs lacking this domain, such as the PspF activator of the sigma(54)-dependent pspA promoter, are constitutively active. We describe here the in vivo and in vitro properties of the PspA protein of Escherichia coli, which negatively regulates expression of the pspA promoter without binding DNA directly.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription",
pages = "319-311",
number = "2",
volume = "182",
doi = "10.1128/JB.182.2.311-319.2000"
}

Dworkin, J., Jovanović, G.,& Model, P.. (2000). The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 182(2), 311-319.
https://doi.org/10.1128/JB.182.2.311-319.2000

Dworkin J, Jovanović G, Model P. The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription. in Journal of Bacteriology. 2000;182(2):311-319.
doi:10.1128/JB.182.2.311-319.2000 .

Dworkin, J, Jovanović, Goran, Model, P, "The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription" in Journal of Bacteriology, 182, no. 2 (2000):311-319,
https://doi.org/10.1128/JB.182.2.311-319.2000 . .

TY  - JOUR
AU  - Model, P
AU  - Jovanović, Goran
AU  - Dworkin, J
PY  - 1997
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/102
AB  - The phage-shock-protein (psp) operon helps to ensure survival of Escherichia coli in tate stationary phase at alkaline pH, and protects the cell against dissipation of its proton-motive force against challenge. It is strongly induced by filamentous phage pIV and its bacterial homologues, and by mutant porins that don't localize properly, as well as by a number of other stresses. Transcription of the operon is dependent on sigma(54) and a constitutively active, autogenously controlled activator. psp-operon expression is controlled by one negatively and several positively acting regulators, none of which is a DNA-binding protein. The major product of the operon, PspA, may also serve as a negative regulator of an unusual porin, OmpG.
PB  - Blackwell Science Ltd, Oxford
T2  - Molecular Microbiology
T1  - The Escherichia coli phage-shock-protein (psp) operon
EP  - 261
IS  - 2
SP  - 255
VL  - 24
DO  - 10.1046/j.1365-2958.1997.3481712.x
ER  - 

@article{
author = "Model, P and Jovanović, Goran and Dworkin, J",
year = "1997",
abstract = "The phage-shock-protein (psp) operon helps to ensure survival of Escherichia coli in tate stationary phase at alkaline pH, and protects the cell against dissipation of its proton-motive force against challenge. It is strongly induced by filamentous phage pIV and its bacterial homologues, and by mutant porins that don't localize properly, as well as by a number of other stresses. Transcription of the operon is dependent on sigma(54) and a constitutively active, autogenously controlled activator. psp-operon expression is controlled by one negatively and several positively acting regulators, none of which is a DNA-binding protein. The major product of the operon, PspA, may also serve as a negative regulator of an unusual porin, OmpG.",
publisher = "Blackwell Science Ltd, Oxford",
journal = "Molecular Microbiology",
title = "The Escherichia coli phage-shock-protein (psp) operon",
pages = "261-255",
number = "2",
volume = "24",
doi = "10.1046/j.1365-2958.1997.3481712.x"
}

Model, P., Jovanović, G.,& Dworkin, J.. (1997). The Escherichia coli phage-shock-protein (psp) operon. in Molecular Microbiology
Blackwell Science Ltd, Oxford., 24(2), 255-261.
https://doi.org/10.1046/j.1365-2958.1997.3481712.x

Model P, Jovanović G, Dworkin J. The Escherichia coli phage-shock-protein (psp) operon. in Molecular Microbiology. 1997;24(2):255-261.
doi:10.1046/j.1365-2958.1997.3481712.x .

Model, P, Jovanović, Goran, Dworkin, J, "The Escherichia coli phage-shock-protein (psp) operon" in Molecular Microbiology, 24, no. 2 (1997):255-261,
https://doi.org/10.1046/j.1365-2958.1997.3481712.x . .

TY  - JOUR
AU  - Jovanović, Goran
AU  - Dworkin, J
AU  - Model, P
PY  - 1997
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/101
AB  - Escherichia coli sigma(54)-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator, sigma(70)-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF Delta HTH. Negative autoregulation of PspF is continual and not affected by stimuli, like f1 pIV, that induce the pspA to -E operon. PspF production is Independent of PspA (the negative regulator of the pspA to -E operon) and of PspB and -C (positive regulators).
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli
EP  - 5237
IS  - 16
SP  - 5232
VL  - 179
DO  - 10.1128/jb.179.16.5232-5237.1997
ER  - 

@article{
author = "Jovanović, Goran and Dworkin, J and Model, P",
year = "1997",
abstract = "Escherichia coli sigma(54)-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator, sigma(70)-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF Delta HTH. Negative autoregulation of PspF is continual and not affected by stimuli, like f1 pIV, that induce the pspA to -E operon. PspF production is Independent of PspA (the negative regulator of the pspA to -E operon) and of PspB and -C (positive regulators).",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli",
pages = "5237-5232",
number = "16",
volume = "179",
doi = "10.1128/jb.179.16.5232-5237.1997"
}

Jovanović, G., Dworkin, J.,& Model, P.. (1997). Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 179(16), 5232-5237.
https://doi.org/10.1128/jb.179.16.5232-5237.1997

Jovanović G, Dworkin J, Model P. Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli. in Journal of Bacteriology. 1997;179(16):5232-5237.
doi:10.1128/jb.179.16.5232-5237.1997 .

Jovanović, Goran, Dworkin, J, Model, P, "Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli" in Journal of Bacteriology, 179, no. 16 (1997):5232-5237,
https://doi.org/10.1128/jb.179.16.5232-5237.1997 . .

TY  - JOUR
AU  - Dworkin, J
AU  - Jovanović, Goran
AU  - Model, P
PY  - 1997
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/107
AB  - PspF, the transcriptional activator of the pspA operon of Escherichia coli, which belongs to the enhancer binding protein (EBP) family of sigma(54) activator proteins, is constitutively active in an in vitro transcription assay. PspF protein, together with RNA polymerase holoenzyme containing sigma(54), is required for in vitro transcription from the pspA promoter. EBP proteins are typically subject to regulation either by post-translational modification or interaction of a specific ligand with an N-terminal regulatory domain. However, unlike other members of the EBP family, PspF lacks this domain. pspA is positively regulated by IHF in vitro, and this regulation is dependent on the topology of the DNA; a linear template is much more dependent on IHF than a supercoiled template. EBP binding to upstream activating sequences (UAS) in their target promoters is mediated by the C-terminal domain which contains a helix-turn-helix DNA-binding motif. A mutant PspF protein lacking the C-terminal DNA-binding domain is active in vitro, although at much higher concentrations than the wild-type protein. In vitro transcription from pspA templates missing one or both of the UAS sites is reduced relative to wild-type templates, but is still appreciable; however, IHF acts as a negative regulator of pspA transcription on these mutant templates. Thus, PspF bound to non-specific sequences upstream of the pspA promoter can activate pspA transcription, but this activation is inhibited by IHF. These data, taken together, support the model that a precise promoter geometry is necessary for IHF to positively regulate transcription and that IHF may act to prevent activation from inappropriately spaced upstream sites.
PB  - Academic Press Ltd, London
T2  - Journal of Molecular Biology
T1  - Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF
EP  - 388
IS  - 2
SP  - 377
VL  - 273
DO  - 10.1006/jmbi.1997.1317
ER  - 

@article{
author = "Dworkin, J and Jovanović, Goran and Model, P",
year = "1997",
abstract = "PspF, the transcriptional activator of the pspA operon of Escherichia coli, which belongs to the enhancer binding protein (EBP) family of sigma(54) activator proteins, is constitutively active in an in vitro transcription assay. PspF protein, together with RNA polymerase holoenzyme containing sigma(54), is required for in vitro transcription from the pspA promoter. EBP proteins are typically subject to regulation either by post-translational modification or interaction of a specific ligand with an N-terminal regulatory domain. However, unlike other members of the EBP family, PspF lacks this domain. pspA is positively regulated by IHF in vitro, and this regulation is dependent on the topology of the DNA; a linear template is much more dependent on IHF than a supercoiled template. EBP binding to upstream activating sequences (UAS) in their target promoters is mediated by the C-terminal domain which contains a helix-turn-helix DNA-binding motif. A mutant PspF protein lacking the C-terminal DNA-binding domain is active in vitro, although at much higher concentrations than the wild-type protein. In vitro transcription from pspA templates missing one or both of the UAS sites is reduced relative to wild-type templates, but is still appreciable; however, IHF acts as a negative regulator of pspA transcription on these mutant templates. Thus, PspF bound to non-specific sequences upstream of the pspA promoter can activate pspA transcription, but this activation is inhibited by IHF. These data, taken together, support the model that a precise promoter geometry is necessary for IHF to positively regulate transcription and that IHF may act to prevent activation from inappropriately spaced upstream sites.",
publisher = "Academic Press Ltd, London",
journal = "Journal of Molecular Biology",
title = "Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF",
pages = "388-377",
number = "2",
volume = "273",
doi = "10.1006/jmbi.1997.1317"
}

Dworkin, J., Jovanović, G.,& Model, P.. (1997). Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF. in Journal of Molecular Biology
Academic Press Ltd, London., 273(2), 377-388.
https://doi.org/10.1006/jmbi.1997.1317

Dworkin J, Jovanović G, Model P. Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF. in Journal of Molecular Biology. 1997;273(2):377-388.
doi:10.1006/jmbi.1997.1317 .

Dworkin, J, Jovanović, Goran, Model, P, "Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF" in Journal of Molecular Biology, 273, no. 2 (1997):377-388,
https://doi.org/10.1006/jmbi.1997.1317 . .