TY  - JOUR
AU  - Dworkin, J
AU  - Jovanović, Goran
AU  - Model, P
PY  - 2000
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/143
AB  - In Eubacteria, expression of genes transcribed by an RNA polymerase holoenzyme containing the alternate sigma factor sigma(54) is positively regulated by proteins belonging to the family of enhancer-binding proteins (EBPs), These proteins bind to upstream activation sequences and are required for the initiation of transcription at the sigma(54)-dependent promoters. They are typically inactive until modified in their N-terminal regulatory domain either by specific phosphorylation or by the binding of a small effector molecule. EBPs lacking this domain, such as the PspF activator of the sigma(54)-dependent pspA promoter, are constitutively active. We describe here the in vivo and in vitro properties of the PspA protein of Escherichia coli, which negatively regulates expression of the pspA promoter without binding DNA directly.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription
EP  - 319
IS  - 2
SP  - 311
VL  - 182
DO  - 10.1128/JB.182.2.311-319.2000
ER  - 

@article{
author = "Dworkin, J and Jovanović, Goran and Model, P",
year = "2000",
abstract = "In Eubacteria, expression of genes transcribed by an RNA polymerase holoenzyme containing the alternate sigma factor sigma(54) is positively regulated by proteins belonging to the family of enhancer-binding proteins (EBPs), These proteins bind to upstream activation sequences and are required for the initiation of transcription at the sigma(54)-dependent promoters. They are typically inactive until modified in their N-terminal regulatory domain either by specific phosphorylation or by the binding of a small effector molecule. EBPs lacking this domain, such as the PspF activator of the sigma(54)-dependent pspA promoter, are constitutively active. We describe here the in vivo and in vitro properties of the PspA protein of Escherichia coli, which negatively regulates expression of the pspA promoter without binding DNA directly.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription",
pages = "319-311",
number = "2",
volume = "182",
doi = "10.1128/JB.182.2.311-319.2000"
}

Dworkin, J., Jovanović, G.,& Model, P.. (2000). The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 182(2), 311-319.
https://doi.org/10.1128/JB.182.2.311-319.2000

Dworkin J, Jovanović G, Model P. The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription. in Journal of Bacteriology. 2000;182(2):311-319.
doi:10.1128/JB.182.2.311-319.2000 .

Dworkin, J, Jovanović, Goran, Model, P, "The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription" in Journal of Bacteriology, 182, no. 2 (2000):311-319,
https://doi.org/10.1128/JB.182.2.311-319.2000 . .

TY  - JOUR
AU  - Jovanović, Goran
AU  - Rakonjac, J
AU  - Model, P
PY  - 1999
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/119
AB  - Transcription of the phage-shock protein (psp) operon in Escherichia coli is driven by a sigma(54) promoter, stimulated by integration host factor and dependent on an upstream, cis-acting sequence and an activator protein, PspF. PspF belongs to the enhancer binding protein family but lacks an N-terminal regulatory domain. Purified PspF is not modified and has an ATPase activity that is increased twofold in the presence of DNA carrying the psp cis-acting sequence. Purified mutant I-Iis-tagged PspF that lacks the C-terminal DNA-binding motif has a DNA-independent ATPase activity when present at 30-fold the concentration of the wild-type protein. Both proteins oligomerize in solution in an ATP and DNA-independent manner. The wild-type activator protein, but not the DNA-binding mutant, binds specifically to the cis-acting sequence. Analysis of the sequence protected by PspF demonstrates the presence of two upstream binding sites within the sequence, UAS I and UAS II, which together constitute the psp enhancer. Protection at low protein concentrations is more pronounced and more extensive on a supercoiled DNA than on a linear template. Full expression of the psp operon upon hyperosmotic shock depends on wild-type PspF, but only partially requires the presence of the psp enhancer.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Journal of Molecular Biology
T1  - In vivo and in vitro activities of the Escherichia coli sigma(54) transcription activator, PspF, and its DNA-binding mutant, PspF Delta HTH
EP  - 483
IS  - 2
SP  - 469
VL  - 285
DO  - 10.1006/jmbi.1998.2263
ER  - 

@article{
author = "Jovanović, Goran and Rakonjac, J and Model, P",
year = "1999",
abstract = "Transcription of the phage-shock protein (psp) operon in Escherichia coli is driven by a sigma(54) promoter, stimulated by integration host factor and dependent on an upstream, cis-acting sequence and an activator protein, PspF. PspF belongs to the enhancer binding protein family but lacks an N-terminal regulatory domain. Purified PspF is not modified and has an ATPase activity that is increased twofold in the presence of DNA carrying the psp cis-acting sequence. Purified mutant I-Iis-tagged PspF that lacks the C-terminal DNA-binding motif has a DNA-independent ATPase activity when present at 30-fold the concentration of the wild-type protein. Both proteins oligomerize in solution in an ATP and DNA-independent manner. The wild-type activator protein, but not the DNA-binding mutant, binds specifically to the cis-acting sequence. Analysis of the sequence protected by PspF demonstrates the presence of two upstream binding sites within the sequence, UAS I and UAS II, which together constitute the psp enhancer. Protection at low protein concentrations is more pronounced and more extensive on a supercoiled DNA than on a linear template. Full expression of the psp operon upon hyperosmotic shock depends on wild-type PspF, but only partially requires the presence of the psp enhancer.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Journal of Molecular Biology",
title = "In vivo and in vitro activities of the Escherichia coli sigma(54) transcription activator, PspF, and its DNA-binding mutant, PspF Delta HTH",
pages = "483-469",
number = "2",
volume = "285",
doi = "10.1006/jmbi.1998.2263"
}

Jovanović, G., Rakonjac, J.,& Model, P.. (1999). In vivo and in vitro activities of the Escherichia coli sigma(54) transcription activator, PspF, and its DNA-binding mutant, PspF Delta HTH. in Journal of Molecular Biology
Academic Press Ltd- Elsevier Science Ltd, London., 285(2), 469-483.
https://doi.org/10.1006/jmbi.1998.2263

Jovanović G, Rakonjac J, Model P. In vivo and in vitro activities of the Escherichia coli sigma(54) transcription activator, PspF, and its DNA-binding mutant, PspF Delta HTH. in Journal of Molecular Biology. 1999;285(2):469-483.
doi:10.1006/jmbi.1998.2263 .

Jovanović, Goran, Rakonjac, J, Model, P, "In vivo and in vitro activities of the Escherichia coli sigma(54) transcription activator, PspF, and its DNA-binding mutant, PspF Delta HTH" in Journal of Molecular Biology, 285, no. 2 (1999):469-483,
https://doi.org/10.1006/jmbi.1998.2263 . .

TY  - JOUR
AU  - Jovanović, Goran
AU  - Model, P
PY  - 1997
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/105
AB  - PspF bound to the psp enhancer activates E sigma(54) holoenzyme-dependent transcription of the Escherichia coli phage-shock protein (psp) operon and autogenously represses its own sigma(70)-dependent transcription, thereby keeping its concentration at a low level. It has been demonstrated previously that integration host factor (IHF) bound to a DNA site located between the psp core promoter and the PspF binding sites stimulates psp expression. We show here that wild-type IHF strongly retards DNA containing the psp promoter region. In vitro, PspF binding to the psp enhancer facilitates IHF binding, while IHF binding to the pspF-pspA-E promoter-regulatory region increases the efficacy of PspF binding to the upstream activating sequences (UASs). This is the first demonstration of co-operative binding of an activator and IHF in a sigma(54)-dependent system. In the absence of IHF, in vivo autoregulation of pspF transcription is lifted and, consequently, PspF production is increased, indicating that IHF enhances PspF binding to the psp enhancer in vivo.
PB  - Wiley-Blackwell, Hoboken
T2  - Molecular Microbiology
T1  - PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli
EP  - 481
IS  - 3
SP  - 473
VL  - 25
DO  - 10.1046/j.1365-2958.1997.4791844.x
ER  - 

@article{
author = "Jovanović, Goran and Model, P",
year = "1997",
abstract = "PspF bound to the psp enhancer activates E sigma(54) holoenzyme-dependent transcription of the Escherichia coli phage-shock protein (psp) operon and autogenously represses its own sigma(70)-dependent transcription, thereby keeping its concentration at a low level. It has been demonstrated previously that integration host factor (IHF) bound to a DNA site located between the psp core promoter and the PspF binding sites stimulates psp expression. We show here that wild-type IHF strongly retards DNA containing the psp promoter region. In vitro, PspF binding to the psp enhancer facilitates IHF binding, while IHF binding to the pspF-pspA-E promoter-regulatory region increases the efficacy of PspF binding to the upstream activating sequences (UASs). This is the first demonstration of co-operative binding of an activator and IHF in a sigma(54)-dependent system. In the absence of IHF, in vivo autoregulation of pspF transcription is lifted and, consequently, PspF production is increased, indicating that IHF enhances PspF binding to the psp enhancer in vivo.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Molecular Microbiology",
title = "PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli",
pages = "481-473",
number = "3",
volume = "25",
doi = "10.1046/j.1365-2958.1997.4791844.x"
}

Jovanović, G.,& Model, P.. (1997). PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli. in Molecular Microbiology
Wiley-Blackwell, Hoboken., 25(3), 473-481.
https://doi.org/10.1046/j.1365-2958.1997.4791844.x

Jovanović G, Model P. PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli. in Molecular Microbiology. 1997;25(3):473-481.
doi:10.1046/j.1365-2958.1997.4791844.x .

Jovanović, Goran, Model, P, "PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli" in Molecular Microbiology, 25, no. 3 (1997):473-481,
https://doi.org/10.1046/j.1365-2958.1997.4791844.x . .

TY  - JOUR
AU  - Model, P
AU  - Jovanović, Goran
AU  - Dworkin, J
PY  - 1997
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/102
AB  - The phage-shock-protein (psp) operon helps to ensure survival of Escherichia coli in tate stationary phase at alkaline pH, and protects the cell against dissipation of its proton-motive force against challenge. It is strongly induced by filamentous phage pIV and its bacterial homologues, and by mutant porins that don't localize properly, as well as by a number of other stresses. Transcription of the operon is dependent on sigma(54) and a constitutively active, autogenously controlled activator. psp-operon expression is controlled by one negatively and several positively acting regulators, none of which is a DNA-binding protein. The major product of the operon, PspA, may also serve as a negative regulator of an unusual porin, OmpG.
PB  - Blackwell Science Ltd, Oxford
T2  - Molecular Microbiology
T1  - The Escherichia coli phage-shock-protein (psp) operon
EP  - 261
IS  - 2
SP  - 255
VL  - 24
DO  - 10.1046/j.1365-2958.1997.3481712.x
ER  - 

@article{
author = "Model, P and Jovanović, Goran and Dworkin, J",
year = "1997",
abstract = "The phage-shock-protein (psp) operon helps to ensure survival of Escherichia coli in tate stationary phase at alkaline pH, and protects the cell against dissipation of its proton-motive force against challenge. It is strongly induced by filamentous phage pIV and its bacterial homologues, and by mutant porins that don't localize properly, as well as by a number of other stresses. Transcription of the operon is dependent on sigma(54) and a constitutively active, autogenously controlled activator. psp-operon expression is controlled by one negatively and several positively acting regulators, none of which is a DNA-binding protein. The major product of the operon, PspA, may also serve as a negative regulator of an unusual porin, OmpG.",
publisher = "Blackwell Science Ltd, Oxford",
journal = "Molecular Microbiology",
title = "The Escherichia coli phage-shock-protein (psp) operon",
pages = "261-255",
number = "2",
volume = "24",
doi = "10.1046/j.1365-2958.1997.3481712.x"
}

Model, P., Jovanović, G.,& Dworkin, J.. (1997). The Escherichia coli phage-shock-protein (psp) operon. in Molecular Microbiology
Blackwell Science Ltd, Oxford., 24(2), 255-261.
https://doi.org/10.1046/j.1365-2958.1997.3481712.x

Model P, Jovanović G, Dworkin J. The Escherichia coli phage-shock-protein (psp) operon. in Molecular Microbiology. 1997;24(2):255-261.
doi:10.1046/j.1365-2958.1997.3481712.x .

Model, P, Jovanović, Goran, Dworkin, J, "The Escherichia coli phage-shock-protein (psp) operon" in Molecular Microbiology, 24, no. 2 (1997):255-261,
https://doi.org/10.1046/j.1365-2958.1997.3481712.x . .

TY  - JOUR
AU  - Rakonjac, J
AU  - Jovanović, Goran
AU  - Model, P
PY  - 1997
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/106
AB  - We describe the use of transcriptional fusions to the phage shock protein (psp) promoter. These fusions are expressed only when cells are infected by filamentous phage. In an application, the psp promoter was fused to the protein coding part of filamentous phage gene III (gIII). Protein III (pIII) is needed to complement mutant fl phage containing a deletion of gIII but its synthesis also renders cells resistant to infection. By inducing pIII production from psp-gIII only in the cells that are already infected with phage, it was possible to obtain plaques from phage in which gill had been completely deleted. gIII was deleted from two helper phages: R408 and VCSM13, which were then propagated on cells containing the psp-gIII fusion. These two phages were tested for use in a phage display method that requires generation of noninfectious, phagemid-containing virion-like particles. Both helpers worked, but R408d3 was superior to VCSM13d3, because it generated about 1800-times fewer background infectious particles.
PB  - Elsevier Science Bv, Amsterdam
T2  - Gene
T1  - Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3
EP  - 103
IS  - 1-2
SP  - 99
VL  - 198
DO  - 10.1016/S0378-1119(97)00298-9
ER  - 

@article{
author = "Rakonjac, J and Jovanović, Goran and Model, P",
year = "1997",
abstract = "We describe the use of transcriptional fusions to the phage shock protein (psp) promoter. These fusions are expressed only when cells are infected by filamentous phage. In an application, the psp promoter was fused to the protein coding part of filamentous phage gene III (gIII). Protein III (pIII) is needed to complement mutant fl phage containing a deletion of gIII but its synthesis also renders cells resistant to infection. By inducing pIII production from psp-gIII only in the cells that are already infected with phage, it was possible to obtain plaques from phage in which gill had been completely deleted. gIII was deleted from two helper phages: R408 and VCSM13, which were then propagated on cells containing the psp-gIII fusion. These two phages were tested for use in a phage display method that requires generation of noninfectious, phagemid-containing virion-like particles. Both helpers worked, but R408d3 was superior to VCSM13d3, because it generated about 1800-times fewer background infectious particles.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Gene",
title = "Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3",
pages = "103-99",
number = "1-2",
volume = "198",
doi = "10.1016/S0378-1119(97)00298-9"
}

Rakonjac, J., Jovanović, G.,& Model, P.. (1997). Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3. in Gene
Elsevier Science Bv, Amsterdam., 198(1-2), 99-103.
https://doi.org/10.1016/S0378-1119(97)00298-9

Rakonjac J, Jovanović G, Model P. Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3. in Gene. 1997;198(1-2):99-103.
doi:10.1016/S0378-1119(97)00298-9 .

Rakonjac, J, Jovanović, Goran, Model, P, "Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3" in Gene, 198, no. 1-2 (1997):99-103,
https://doi.org/10.1016/S0378-1119(97)00298-9 . .

TY  - JOUR
AU  - Jovanović, Goran
AU  - Dworkin, J
AU  - Model, P
PY  - 1997
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/101
AB  - Escherichia coli sigma(54)-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator, sigma(70)-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF Delta HTH. Negative autoregulation of PspF is continual and not affected by stimuli, like f1 pIV, that induce the pspA to -E operon. PspF production is Independent of PspA (the negative regulator of the pspA to -E operon) and of PspB and -C (positive regulators).
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli
EP  - 5237
IS  - 16
SP  - 5232
VL  - 179
DO  - 10.1128/jb.179.16.5232-5237.1997
ER  - 

@article{
author = "Jovanović, Goran and Dworkin, J and Model, P",
year = "1997",
abstract = "Escherichia coli sigma(54)-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator, sigma(70)-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF Delta HTH. Negative autoregulation of PspF is continual and not affected by stimuli, like f1 pIV, that induce the pspA to -E operon. PspF production is Independent of PspA (the negative regulator of the pspA to -E operon) and of PspB and -C (positive regulators).",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli",
pages = "5237-5232",
number = "16",
volume = "179",
doi = "10.1128/jb.179.16.5232-5237.1997"
}

Jovanović, G., Dworkin, J.,& Model, P.. (1997). Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 179(16), 5232-5237.
https://doi.org/10.1128/jb.179.16.5232-5237.1997

Jovanović G, Dworkin J, Model P. Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli. in Journal of Bacteriology. 1997;179(16):5232-5237.
doi:10.1128/jb.179.16.5232-5237.1997 .

Jovanović, Goran, Dworkin, J, Model, P, "Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli" in Journal of Bacteriology, 179, no. 16 (1997):5232-5237,
https://doi.org/10.1128/jb.179.16.5232-5237.1997 . .

TY  - JOUR
AU  - Dworkin, J
AU  - Jovanović, Goran
AU  - Model, P
PY  - 1997
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/107
AB  - PspF, the transcriptional activator of the pspA operon of Escherichia coli, which belongs to the enhancer binding protein (EBP) family of sigma(54) activator proteins, is constitutively active in an in vitro transcription assay. PspF protein, together with RNA polymerase holoenzyme containing sigma(54), is required for in vitro transcription from the pspA promoter. EBP proteins are typically subject to regulation either by post-translational modification or interaction of a specific ligand with an N-terminal regulatory domain. However, unlike other members of the EBP family, PspF lacks this domain. pspA is positively regulated by IHF in vitro, and this regulation is dependent on the topology of the DNA; a linear template is much more dependent on IHF than a supercoiled template. EBP binding to upstream activating sequences (UAS) in their target promoters is mediated by the C-terminal domain which contains a helix-turn-helix DNA-binding motif. A mutant PspF protein lacking the C-terminal DNA-binding domain is active in vitro, although at much higher concentrations than the wild-type protein. In vitro transcription from pspA templates missing one or both of the UAS sites is reduced relative to wild-type templates, but is still appreciable; however, IHF acts as a negative regulator of pspA transcription on these mutant templates. Thus, PspF bound to non-specific sequences upstream of the pspA promoter can activate pspA transcription, but this activation is inhibited by IHF. These data, taken together, support the model that a precise promoter geometry is necessary for IHF to positively regulate transcription and that IHF may act to prevent activation from inappropriately spaced upstream sites.
PB  - Academic Press Ltd, London
T2  - Journal of Molecular Biology
T1  - Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF
EP  - 388
IS  - 2
SP  - 377
VL  - 273
DO  - 10.1006/jmbi.1997.1317
ER  - 

@article{
author = "Dworkin, J and Jovanović, Goran and Model, P",
year = "1997",
abstract = "PspF, the transcriptional activator of the pspA operon of Escherichia coli, which belongs to the enhancer binding protein (EBP) family of sigma(54) activator proteins, is constitutively active in an in vitro transcription assay. PspF protein, together with RNA polymerase holoenzyme containing sigma(54), is required for in vitro transcription from the pspA promoter. EBP proteins are typically subject to regulation either by post-translational modification or interaction of a specific ligand with an N-terminal regulatory domain. However, unlike other members of the EBP family, PspF lacks this domain. pspA is positively regulated by IHF in vitro, and this regulation is dependent on the topology of the DNA; a linear template is much more dependent on IHF than a supercoiled template. EBP binding to upstream activating sequences (UAS) in their target promoters is mediated by the C-terminal domain which contains a helix-turn-helix DNA-binding motif. A mutant PspF protein lacking the C-terminal DNA-binding domain is active in vitro, although at much higher concentrations than the wild-type protein. In vitro transcription from pspA templates missing one or both of the UAS sites is reduced relative to wild-type templates, but is still appreciable; however, IHF acts as a negative regulator of pspA transcription on these mutant templates. Thus, PspF bound to non-specific sequences upstream of the pspA promoter can activate pspA transcription, but this activation is inhibited by IHF. These data, taken together, support the model that a precise promoter geometry is necessary for IHF to positively regulate transcription and that IHF may act to prevent activation from inappropriately spaced upstream sites.",
publisher = "Academic Press Ltd, London",
journal = "Journal of Molecular Biology",
title = "Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF",
pages = "388-377",
number = "2",
volume = "273",
doi = "10.1006/jmbi.1997.1317"
}

Dworkin, J., Jovanović, G.,& Model, P.. (1997). Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF. in Journal of Molecular Biology
Academic Press Ltd, London., 273(2), 377-388.
https://doi.org/10.1006/jmbi.1997.1317

Dworkin J, Jovanović G, Model P. Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF. in Journal of Molecular Biology. 1997;273(2):377-388.
doi:10.1006/jmbi.1997.1317 .

Dworkin, J, Jovanović, Goran, Model, P, "Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF" in Journal of Molecular Biology, 273, no. 2 (1997):377-388,
https://doi.org/10.1006/jmbi.1997.1317 . .

TY  - JOUR
AU  - Jovanović, Goran
AU  - Model, P
PY  - 1997
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/109
AB  - The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined, The pspF gene encodes a sigma(54) transcriptional activator of the phage shock protein (psp) operon (pspA to pspE), Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL, and goaG, convergently oriented with respect to pspF, These two ORFs, together with the adjacent aldH gene, may constitute a novel operon (aldH-ordL-goaG), The goaG-pspF intergenic region contains a complex extragenic mosaic element, RIB, The structure of this RIB element, which belongs to the BIME-1 family, is Y(REP1)  gt  16  lt  Z(1)(REP2), where Y and Z(1) are palindromic units and the central 16 bases contain an L motif with an ihf consensus sequence. DNA fragments containing the L motif of the psp RIB element effectively bind integration host factor (IHF), while the Y palindromic unit (REP1) of the same RIB element binds DNA gyrase weakly. Computer prediction of the pspF mRNA secondary structure suggested that the transcribed stem-loop structures formed by the 3'-flanking region of the pspF transcript containing the RIB element can stabilize and protect pspF mRNA, Analysis of pspF steady-state mRNA levels showed that transcripts,vith an intact RIB element are much more abundant than those truncated at the 3' end by deletion of either the entire RIB element or a single Z(1) sequence (REP2), Thus, the pspF 3'-flanking region containing the RIB element has an important role in the stabilization of the pspF transcript.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - The RIB element in the goaG-pspF intergenic region of Escherichia coli
EP  - 3102
IS  - 10
SP  - 3095
VL  - 179
DO  - 10.1128/jb.179.10.3095-3102.1997
ER  - 

@article{
author = "Jovanović, Goran and Model, P",
year = "1997",
abstract = "The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined, The pspF gene encodes a sigma(54) transcriptional activator of the phage shock protein (psp) operon (pspA to pspE), Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL, and goaG, convergently oriented with respect to pspF, These two ORFs, together with the adjacent aldH gene, may constitute a novel operon (aldH-ordL-goaG), The goaG-pspF intergenic region contains a complex extragenic mosaic element, RIB, The structure of this RIB element, which belongs to the BIME-1 family, is Y(REP1)  gt  16  lt  Z(1)(REP2), where Y and Z(1) are palindromic units and the central 16 bases contain an L motif with an ihf consensus sequence. DNA fragments containing the L motif of the psp RIB element effectively bind integration host factor (IHF), while the Y palindromic unit (REP1) of the same RIB element binds DNA gyrase weakly. Computer prediction of the pspF mRNA secondary structure suggested that the transcribed stem-loop structures formed by the 3'-flanking region of the pspF transcript containing the RIB element can stabilize and protect pspF mRNA, Analysis of pspF steady-state mRNA levels showed that transcripts,vith an intact RIB element are much more abundant than those truncated at the 3' end by deletion of either the entire RIB element or a single Z(1) sequence (REP2), Thus, the pspF 3'-flanking region containing the RIB element has an important role in the stabilization of the pspF transcript.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "The RIB element in the goaG-pspF intergenic region of Escherichia coli",
pages = "3102-3095",
number = "10",
volume = "179",
doi = "10.1128/jb.179.10.3095-3102.1997"
}

Jovanović, G.,& Model, P.. (1997). The RIB element in the goaG-pspF intergenic region of Escherichia coli. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 179(10), 3095-3102.
https://doi.org/10.1128/jb.179.10.3095-3102.1997

Jovanović G, Model P. The RIB element in the goaG-pspF intergenic region of Escherichia coli. in Journal of Bacteriology. 1997;179(10):3095-3102.
doi:10.1128/jb.179.10.3095-3102.1997 .

Jovanović, Goran, Model, P, "The RIB element in the goaG-pspF intergenic region of Escherichia coli" in Journal of Bacteriology, 179, no. 10 (1997):3095-3102,
https://doi.org/10.1128/jb.179.10.3095-3102.1997 . .

TY  - JOUR
AU  - Jovanović, Goran
AU  - Weiner, L
AU  - Model, P
PY  - 1996
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/95
AB  - The phage shock protein (psp) operon (pspABCE) of Escherichia coli is strongly induced in response to a variety of stressful conditions or agents such as filamentous phage infection, ethanol treatment, osmotic shock, heat shock, and prolonged incubation in stationary phase. Transcription of the psp operon is driven from a sigma(54) promoter and stimulated by integration host factor. We report here the identification of a transcriptional activator gene, designated pspF, which controls expression of the psp operon in E. coli. The pspF gene was identified by random miniTn10-tet transposon mutagenesis. Insertion of the transposon into the pspF gene abolished sigma(54)-dependent induction of the psp operon. The pspF gene is closely linked to the psp operon and is divergently transcribed from one major and two minor sigma(70) promoters. pspF encodes a 37-kDa protein which belongs to the enhancer-binding protein family of sigma(54) transcriptional activators. PspF contains a catalytic domain, which in other sigma(54) activators mould be the central domain, and a C-terminal DNA-binding domain but entirely lacks an N-terminal regulatory domain and is constitutively active. The insertion mutant pspF::mTn10-tet (pspF(877)) encodes a truncated protein (PspF Delta HTH) that lacks the DNA-binding helix-turn-helix (HTH) motif. Although the central catalytic domain is intact, PspF Delta HTH at physiological concentration cannot activate psp expression. In the absence of inducing stimuli, multicopy-plasmid-borne PspF or PspF Delta HTH overcomes repression of the psp operon mediated by the negative regulator PspA.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon
EP  - 1945
IS  - 7
SP  - 1936
VL  - 178
DO  - 10.1128/jb.178.7.1936-1945.1996
ER  - 

@article{
author = "Jovanović, Goran and Weiner, L and Model, P",
year = "1996",
abstract = "The phage shock protein (psp) operon (pspABCE) of Escherichia coli is strongly induced in response to a variety of stressful conditions or agents such as filamentous phage infection, ethanol treatment, osmotic shock, heat shock, and prolonged incubation in stationary phase. Transcription of the psp operon is driven from a sigma(54) promoter and stimulated by integration host factor. We report here the identification of a transcriptional activator gene, designated pspF, which controls expression of the psp operon in E. coli. The pspF gene was identified by random miniTn10-tet transposon mutagenesis. Insertion of the transposon into the pspF gene abolished sigma(54)-dependent induction of the psp operon. The pspF gene is closely linked to the psp operon and is divergently transcribed from one major and two minor sigma(70) promoters. pspF encodes a 37-kDa protein which belongs to the enhancer-binding protein family of sigma(54) transcriptional activators. PspF contains a catalytic domain, which in other sigma(54) activators mould be the central domain, and a C-terminal DNA-binding domain but entirely lacks an N-terminal regulatory domain and is constitutively active. The insertion mutant pspF::mTn10-tet (pspF(877)) encodes a truncated protein (PspF Delta HTH) that lacks the DNA-binding helix-turn-helix (HTH) motif. Although the central catalytic domain is intact, PspF Delta HTH at physiological concentration cannot activate psp expression. In the absence of inducing stimuli, multicopy-plasmid-borne PspF or PspF Delta HTH overcomes repression of the psp operon mediated by the negative regulator PspA.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon",
pages = "1945-1936",
number = "7",
volume = "178",
doi = "10.1128/jb.178.7.1936-1945.1996"
}

Jovanović, G., Weiner, L.,& Model, P.. (1996). Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 178(7), 1936-1945.
https://doi.org/10.1128/jb.178.7.1936-1945.1996

Jovanović G, Weiner L, Model P. Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon. in Journal of Bacteriology. 1996;178(7):1936-1945.
doi:10.1128/jb.178.7.1936-1945.1996 .

Jovanović, Goran, Weiner, L, Model, P, "Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon" in Journal of Bacteriology, 178, no. 7 (1996):1936-1945,
https://doi.org/10.1128/jb.178.7.1936-1945.1996 . .