TY  - JOUR
AU  - Simpson, JL
AU  - Morić, Ivana
AU  - Wark, PAB
AU  - Johnston, SL
AU  - Gibson, PG
PY  - 2003
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/180
AB  - Background: Influenza (Flu) and respiratory syncytial virus (RSV) are important viral pathogens that cause lower respiratory tract infections and severe exacerbations of asthma. Molecular biological techniques are permitting a rapid and accurate diagnosis of infections caused by respiratory pathogens, and have typically been applied to upper respiratory samples. Sputum induction provides an opportunity to directly sample secretions from the lower respiratory tract. Objectives/study design: To determine the role of induced sputum reverse-transcription polymerase chain reaction (RT-PCR) in the detection of respiratory pathogens and compare this with detection using serology and immunofluorescent antigen (IFA) testing, we recruited 49 adults from emergency room with exacerbations of asthma. After a medical assessment and spirometry, sputum was induced using ultrasonically nebulised normal saline. Sputum was assayed using IFA and RT-PCR for flu and RSV. Flu serology was performed acutely and at convalescence, 4-5 weeks later. Results: Influenza A or B was detected in 24% of the samples by PCR, significantly more than the nine cases detected using serology and the one case using IFA (P  lt  0.05). RSV was detected in 37% of samples using PCR and 20% by IFA (P  lt  0.05). Conclusion: The combination of induced sputum and RT-PCR provides a useful means of detecting respiratory infection. The technique is safe in both adults and children, and RT-PCR is more sensitive than conventional serology and IFA. The improved sensitivity of induced sputum RT-PCR also permits a more rapid diagnosis and the opportunity of early administration of effective treatments.
PB  - Elsevier, Amsterdam
T2  - Journal of Clinical Virology
T1  - Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques
EP  - 346
IS  - 3
SP  - 339
VL  - 26
DO  - 10.1016/S1386-6532(02)00084-7
ER  - 

@article{
author = "Simpson, JL and Morić, Ivana and Wark, PAB and Johnston, SL and Gibson, PG",
year = "2003",
abstract = "Background: Influenza (Flu) and respiratory syncytial virus (RSV) are important viral pathogens that cause lower respiratory tract infections and severe exacerbations of asthma. Molecular biological techniques are permitting a rapid and accurate diagnosis of infections caused by respiratory pathogens, and have typically been applied to upper respiratory samples. Sputum induction provides an opportunity to directly sample secretions from the lower respiratory tract. Objectives/study design: To determine the role of induced sputum reverse-transcription polymerase chain reaction (RT-PCR) in the detection of respiratory pathogens and compare this with detection using serology and immunofluorescent antigen (IFA) testing, we recruited 49 adults from emergency room with exacerbations of asthma. After a medical assessment and spirometry, sputum was induced using ultrasonically nebulised normal saline. Sputum was assayed using IFA and RT-PCR for flu and RSV. Flu serology was performed acutely and at convalescence, 4-5 weeks later. Results: Influenza A or B was detected in 24% of the samples by PCR, significantly more than the nine cases detected using serology and the one case using IFA (P  lt  0.05). RSV was detected in 37% of samples using PCR and 20% by IFA (P  lt  0.05). Conclusion: The combination of induced sputum and RT-PCR provides a useful means of detecting respiratory infection. The technique is safe in both adults and children, and RT-PCR is more sensitive than conventional serology and IFA. The improved sensitivity of induced sputum RT-PCR also permits a more rapid diagnosis and the opportunity of early administration of effective treatments.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Clinical Virology",
title = "Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques",
pages = "346-339",
number = "3",
volume = "26",
doi = "10.1016/S1386-6532(02)00084-7"
}

Simpson, J., Morić, I., Wark, P., Johnston, S.,& Gibson, P.. (2003). Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques. in Journal of Clinical Virology
Elsevier, Amsterdam., 26(3), 339-346.
https://doi.org/10.1016/S1386-6532(02)00084-7

Simpson J, Morić I, Wark P, Johnston S, Gibson P. Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques. in Journal of Clinical Virology. 2003;26(3):339-346.
doi:10.1016/S1386-6532(02)00084-7 .

Simpson, JL, Morić, Ivana, Wark, PAB, Johnston, SL, Gibson, PG, "Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques" in Journal of Clinical Virology, 26, no. 3 (2003):339-346,
https://doi.org/10.1016/S1386-6532(02)00084-7 . .

TY  - JOUR
AU  - Wark, PAB
AU  - Johnston, SL
AU  - Morić, Ivana
AU  - Simpson, JL
AU  - Hensley, MJ
AU  - Gibson, PG
PY  - 2002
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/169
AB  - Acute exacerbations of asthma are frequently caused by viral infections, but the inflammatory mechanisms in virus-induced asthma are poorly understood. The aim of the present study was to determine whether viral infection in acute asthma was associated with increased sputum neutrophil degranulation and increased cellular lysis and whether these changes are related to clinical severity. Adults (n=49) presenting to the emergency department with acute asthma were examined for infection by means of sputum direct-fluorescence antigen detection, sputum culture, and sputum polymerase chain reaction for Mycoplasma, Chlamydia and Legionella pneumophila, and all common respiratory viruses. Subjects infected with one of these agents were classed as having an infective exacerbation. Spirometry and sputum induction were performed on presentation and 4-5 weeks later. Thirty-seven subjects (76%) had virus infection and acute asthma. Those with virus infection had increased sputum neutrophils (p lt 0.05) and increased neutrophil elastase (p lt 0.05), this was related to increased elevated sputum lactate dehydrogenase (LDH). Subjects with noninfective asthma had an increase in the proportion of sputum eosinophils. Both groups had elevated sputum eosinophil cationic protein (ECP) concentrations. Higher levels of sputum LDH and ECP were associated with a longer hospital stay. Virus infection and acute asthma is associated with neutrophilic inflammation, cell lysis and more severe clinical disease.
PB  - European Respiratory Soc Journals Ltd, Sheffield
T2  - European Respiratory Journal
T1  - Neutrophil degranulation and cell lysis is associated with clinical severity in virus-induced asthma
EP  - 75
IS  - 1
SP  - 68
VL  - 19
DO  - 10.1183/09031936.02.00226302
ER  - 

@article{
author = "Wark, PAB and Johnston, SL and Morić, Ivana and Simpson, JL and Hensley, MJ and Gibson, PG",
year = "2002",
abstract = "Acute exacerbations of asthma are frequently caused by viral infections, but the inflammatory mechanisms in virus-induced asthma are poorly understood. The aim of the present study was to determine whether viral infection in acute asthma was associated with increased sputum neutrophil degranulation and increased cellular lysis and whether these changes are related to clinical severity. Adults (n=49) presenting to the emergency department with acute asthma were examined for infection by means of sputum direct-fluorescence antigen detection, sputum culture, and sputum polymerase chain reaction for Mycoplasma, Chlamydia and Legionella pneumophila, and all common respiratory viruses. Subjects infected with one of these agents were classed as having an infective exacerbation. Spirometry and sputum induction were performed on presentation and 4-5 weeks later. Thirty-seven subjects (76%) had virus infection and acute asthma. Those with virus infection had increased sputum neutrophils (p lt 0.05) and increased neutrophil elastase (p lt 0.05), this was related to increased elevated sputum lactate dehydrogenase (LDH). Subjects with noninfective asthma had an increase in the proportion of sputum eosinophils. Both groups had elevated sputum eosinophil cationic protein (ECP) concentrations. Higher levels of sputum LDH and ECP were associated with a longer hospital stay. Virus infection and acute asthma is associated with neutrophilic inflammation, cell lysis and more severe clinical disease.",
publisher = "European Respiratory Soc Journals Ltd, Sheffield",
journal = "European Respiratory Journal",
title = "Neutrophil degranulation and cell lysis is associated with clinical severity in virus-induced asthma",
pages = "75-68",
number = "1",
volume = "19",
doi = "10.1183/09031936.02.00226302"
}

Wark, P., Johnston, S., Morić, I., Simpson, J., Hensley, M.,& Gibson, P.. (2002). Neutrophil degranulation and cell lysis is associated with clinical severity in virus-induced asthma. in European Respiratory Journal
European Respiratory Soc Journals Ltd, Sheffield., 19(1), 68-75.
https://doi.org/10.1183/09031936.02.00226302

Wark P, Johnston S, Morić I, Simpson J, Hensley M, Gibson P. Neutrophil degranulation and cell lysis is associated with clinical severity in virus-induced asthma. in European Respiratory Journal. 2002;19(1):68-75.
doi:10.1183/09031936.02.00226302 .

Wark, PAB, Johnston, SL, Morić, Ivana, Simpson, JL, Hensley, MJ, Gibson, PG, "Neutrophil degranulation and cell lysis is associated with clinical severity in virus-induced asthma" in European Respiratory Journal, 19, no. 1 (2002):68-75,
https://doi.org/10.1183/09031936.02.00226302 . .