Petrović, Jelena

Link to this page

Authority KeyName Variants
30413b4a-1e9a-48cc-9d54-408053375fe5
  • Petrović, Jelena (6)

Author's Bibliography

Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model

Petrović, Jelena; Pańczyszyn, Elżbieta; Corazzari, Marco; Banićević, Ivana; Milivojević, Milena; Bojić, Luka; Stevanović, Milena; Dragoj, Miodrag; Pešić, Milica; Janković, Radmila; Obradović, Bojana; Stojkovska, Jasmina

(2024)

TY  - CONF
AU  - Petrović, Jelena
AU  - Pańczyszyn, Elżbieta
AU  - Corazzari, Marco
AU  - Banićević, Ivana
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Stevanović, Milena
AU  - Dragoj, Miodrag
AU  - Pešić, Milica
AU  - Janković, Radmila
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1264
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2364
AB  - The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.
C3  - Hemijska industrija (Chemical Industry)
T1  - Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model
EP  - 21
IS  - 1S
SP  - 21
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2364
ER  - 
@conference{
author = "Petrović, Jelena and Pańczyszyn, Elżbieta and Corazzari, Marco and Banićević, Ivana and Milivojević, Milena and Bojić, Luka and Stevanović, Milena and Dragoj, Miodrag and Pešić, Milica and Janković, Radmila and Obradović, Bojana and Stojkovska, Jasmina",
year = "2024",
abstract = "The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model",
pages = "21-21",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2364"
}
Petrović, J., Pańczyszyn, E., Corazzari, M., Banićević, I., Milivojević, M., Bojić, L., Stevanović, M., Dragoj, M., Pešić, M., Janković, R., Obradović, B.,& Stojkovska, J.. (2024). Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry), 78(1S), 21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364
Petrović J, Pańczyszyn E, Corazzari M, Banićević I, Milivojević M, Bojić L, Stevanović M, Dragoj M, Pešić M, Janković R, Obradović B, Stojkovska J. Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry). 2024;78(1S):21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364 .
Petrović, Jelena, Pańczyszyn, Elżbieta, Corazzari, Marco, Banićević, Ivana, Milivojević, Milena, Bojić, Luka, Stevanović, Milena, Dragoj, Miodrag, Pešić, Milica, Janković, Radmila, Obradović, Bojana, Stojkovska, Jasmina, "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):21-21,
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research

Banićević, Ivana; Milošević, Mia; Petrović, Jelena; Menshikh, Ksenia; Milivojević, Milena; Stevanović, Milena; Janković, Radmila; Cochis, Andrea; Bella, Elena Della; Stoddart, Martin; Rimondini, Lia; Stojkovska, Jasmina; Obradović, Bojana

(2024)

TY  - CONF
AU  - Banićević, Ivana
AU  - Milošević, Mia
AU  - Petrović, Jelena
AU  - Menshikh, Ksenia
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Janković, Radmila
AU  - Cochis, Andrea
AU  - Bella, Elena Della
AU  - Stoddart, Martin
AU  - Rimondini, Lia
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1261
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2363
AB  - Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.
C3  - Hemijska industrija (Chemical Industry)
T1  - A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research
EP  - 19
IS  - 1S
SP  - 19
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2363
ER  - 
@conference{
author = "Banićević, Ivana and Milošević, Mia and Petrović, Jelena and Menshikh, Ksenia and Milivojević, Milena and Stevanović, Milena and Janković, Radmila and Cochis, Andrea and Bella, Elena Della and Stoddart, Martin and Rimondini, Lia and Stojkovska, Jasmina and Obradović, Bojana",
year = "2024",
abstract = "Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.",
journal = "Hemijska industrija (Chemical Industry)",
title = "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research",
pages = "19-19",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2363"
}
Banićević, I., Milošević, M., Petrović, J., Menshikh, K., Milivojević, M., Stevanović, M., Janković, R., Cochis, A., Bella, E. D., Stoddart, M., Rimondini, L., Stojkovska, J.,& Obradović, B.. (2024). A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry), 78(1S), 19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363
Banićević I, Milošević M, Petrović J, Menshikh K, Milivojević M, Stevanović M, Janković R, Cochis A, Bella ED, Stoddart M, Rimondini L, Stojkovska J, Obradović B. A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry). 2024;78(1S):19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363 .
Banićević, Ivana, Milošević, Mia, Petrović, Jelena, Menshikh, Ksenia, Milivojević, Milena, Stevanović, Milena, Janković, Radmila, Cochis, Andrea, Bella, Elena Della, Stoddart, Martin, Rimondini, Lia, Stojkovska, Jasmina, Obradović, Bojana, "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):19-19,
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations

Radonjić, Mia; Petrović, Jelena; Milivojević, Milena; Stevanović, Milena; Stojkovska, Jasmina; Obradović, Bojana

(Savez hemijskih inženjera, Beograd, 2022)

TY  - JOUR
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1594
AB  - A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 mu m s(-1) induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as similar to 67 mPa. On the contrary, similar velocity (100 mu m s(-1)) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of similar to 10 nm s(-1)). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of similar to 10(-)(19)-10(-11) m(2) s(-)(1) are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (similar to 10(-1)(9) m(2) s(-1)) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations
EP  - 223
IS  - 3
SP  - 211
VL  - 28
DO  - 10.2298/CICEQ210607033R
ER  - 
@article{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
abstract = "A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 mu m s(-1) induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as similar to 67 mPa. On the contrary, similar velocity (100 mu m s(-1)) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of similar to 10 nm s(-1)). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of similar to 10(-)(19)-10(-11) m(2) s(-)(1) are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (similar to 10(-1)(9) m(2) s(-1)) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations",
pages = "223-211",
number = "3",
volume = "28",
doi = "10.2298/CICEQ210607033R"
}
Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 28(3), 211-223.
https://doi.org/10.2298/CICEQ210607033R
Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations. in Chemical Industry & Chemical Engineering Quarterly. 2022;28(3):211-223.
doi:10.2298/CICEQ210607033R .
Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations" in Chemical Industry & Chemical Engineering Quarterly, 28, no. 3 (2022):211-223,
https://doi.org/10.2298/CICEQ210607033R . .
2
1

Optimization of 3d cancer cell culture conditions by application of chemical engineering principles

Radonjić, Mia; Petrović, Jelena; Milivojević, Milena; Stevanović, Milena; Stojkovska, Jasmina; Obradović, Bojana

(Mary Ann Liebert, Inc, New Rochelle, 2022)

TY  - CONF
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1586
PB  - Mary Ann Liebert, Inc, New Rochelle
C3  - Tissue Engineering Part A
T1  - Optimization of 3d cancer cell culture conditions by application of chemical engineering principles
EP  - S395
SP  - S395
VL  - 28
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1586
ER  - 
@conference{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Tissue Engineering Part A",
title = "Optimization of 3d cancer cell culture conditions by application of chemical engineering principles",
pages = "S395-S395",
volume = "28",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1586"
}
Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Optimization of 3d cancer cell culture conditions by application of chemical engineering principles. in Tissue Engineering Part A
Mary Ann Liebert, Inc, New Rochelle., 28, S395-S395.
https://hdl.handle.net/21.15107/rcub_imagine_1586
Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Optimization of 3d cancer cell culture conditions by application of chemical engineering principles. in Tissue Engineering Part A. 2022;28:S395-S395.
https://hdl.handle.net/21.15107/rcub_imagine_1586 .
Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Optimization of 3d cancer cell culture conditions by application of chemical engineering principles" in Tissue Engineering Part A, 28 (2022):S395-S395,
https://hdl.handle.net/21.15107/rcub_imagine_1586 .

Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase

Radovanović, Neda; Malagurski, Ivana; Lević, Steva; Gordić, Milan; Petrović, Jelena; Pavlović, Vladimir; Mitrić, Miodrag; Nesić, Aleksandra; Dimitrijević-Branković, Suzana

(Pergamon-Elsevier Science Ltd, Oxford, 2019)

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Gordić, Milan
AU  - Petrović, Jelena
AU  - Pavlović, Vladimir
AU  - Mitrić, Miodrag
AU  - Nesić, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1200
AB  - New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - European Polymer Journal
T1  - Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase
EP  - 358
SP  - 352
VL  - 119
DO  - 10.1016/j.eurpolymj.2019.08.004
ER  - 
@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Gordić, Milan and Petrović, Jelena and Pavlović, Vladimir and Mitrić, Miodrag and Nesić, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "European Polymer Journal",
title = "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase",
pages = "358-352",
volume = "119",
doi = "10.1016/j.eurpolymj.2019.08.004"
}
Radovanović, N., Malagurski, I., Lević, S., Gordić, M., Petrović, J., Pavlović, V., Mitrić, M., Nesić, A.,& Dimitrijević-Branković, S.. (2019). Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal
Pergamon-Elsevier Science Ltd, Oxford., 119, 352-358.
https://doi.org/10.1016/j.eurpolymj.2019.08.004
Radovanović N, Malagurski I, Lević S, Gordić M, Petrović J, Pavlović V, Mitrić M, Nesić A, Dimitrijević-Branković S. Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal. 2019;119:352-358.
doi:10.1016/j.eurpolymj.2019.08.004 .
Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Gordić, Milan, Petrović, Jelena, Pavlović, Vladimir, Mitrić, Miodrag, Nesić, Aleksandra, Dimitrijević-Branković, Suzana, "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase" in European Polymer Journal, 119 (2019):352-358,
https://doi.org/10.1016/j.eurpolymj.2019.08.004 . .
3
7
5
8

Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer

Nikolić, Aleksandra; Vlajnić, Marina; Ristanović, Momcilo; Petrović, Jelena; Dimitrijević, Ivan; Krivokapić, Zoran; Radojković, Dragica

(Balkan Union of Oncology (B.U.ON.), 2017)

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Vlajnić, Marina
AU  - Ristanović, Momcilo
AU  - Petrović, Jelena
AU  - Dimitrijević, Ivan
AU  - Krivokapić, Zoran
AU  - Radojković, Dragica
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1036
AB  - Purpose: To analyze if cell free (cf)DNA levels and the presence of KRAS and BRAF mutations in serum could be used as diagnostic biomarkers in patients with primary colorectal cancer (CRC). Methods: This study included 92 individuals who were operated due to primary CRC (N=52;study group) and to hemorrhoids (N=40;control group). Serum cfDNA levels were measured with real-time PCR (RT-PCR) using PicoGreen dsDNA quantitation reagent. Colorectal tissue and related blood and serum samples taken at the time of surgery were subjected to DNA extraction and analysis of KRAS and BRAF mutations based on multiplex SNaPshot assay and DNA sequencing. Results: The average cfDNA concentration was lower in patients of the study group (20 +/- 7 ng/mu L) in comparison to controls (34 +/- 9 ng/mu L) and this difference was statistically significant (p lt 0.001). The SNaPshot analysis detected KRAS c35 mutations in colorectal tumor tissue in 14 cases, but the presence of the mutation was not confirmed in cfD-NA extracted from blood samples of these patients. Conclusions: The level of serum cfDNA in CRC is decreased in comparison to patients with hemorrhoids, which questions the usefulness of cfDNA as cancer biomarker. Also, cfDNA does not appear to be suitable as a source for mutation detection in this disease.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of Buon
T1  - Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer
EP  - 183
IS  - 1
SP  - 178
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1036
ER  - 
@article{
author = "Nikolić, Aleksandra and Vlajnić, Marina and Ristanović, Momcilo and Petrović, Jelena and Dimitrijević, Ivan and Krivokapić, Zoran and Radojković, Dragica",
year = "2017",
abstract = "Purpose: To analyze if cell free (cf)DNA levels and the presence of KRAS and BRAF mutations in serum could be used as diagnostic biomarkers in patients with primary colorectal cancer (CRC). Methods: This study included 92 individuals who were operated due to primary CRC (N=52;study group) and to hemorrhoids (N=40;control group). Serum cfDNA levels were measured with real-time PCR (RT-PCR) using PicoGreen dsDNA quantitation reagent. Colorectal tissue and related blood and serum samples taken at the time of surgery were subjected to DNA extraction and analysis of KRAS and BRAF mutations based on multiplex SNaPshot assay and DNA sequencing. Results: The average cfDNA concentration was lower in patients of the study group (20 +/- 7 ng/mu L) in comparison to controls (34 +/- 9 ng/mu L) and this difference was statistically significant (p lt 0.001). The SNaPshot analysis detected KRAS c35 mutations in colorectal tumor tissue in 14 cases, but the presence of the mutation was not confirmed in cfD-NA extracted from blood samples of these patients. Conclusions: The level of serum cfDNA in CRC is decreased in comparison to patients with hemorrhoids, which questions the usefulness of cfDNA as cancer biomarker. Also, cfDNA does not appear to be suitable as a source for mutation detection in this disease.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of Buon",
title = "Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer",
pages = "183-178",
number = "1",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1036"
}
Nikolić, A., Vlajnić, M., Ristanović, M., Petrović, J., Dimitrijević, I., Krivokapić, Z.,& Radojković, D.. (2017). Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer. in Journal of Buon
Balkan Union of Oncology (B.U.ON.)., 22(1), 178-183.
https://hdl.handle.net/21.15107/rcub_imagine_1036
Nikolić A, Vlajnić M, Ristanović M, Petrović J, Dimitrijević I, Krivokapić Z, Radojković D. Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer. in Journal of Buon. 2017;22(1):178-183.
https://hdl.handle.net/21.15107/rcub_imagine_1036 .
Nikolić, Aleksandra, Vlajnić, Marina, Ristanović, Momcilo, Petrović, Jelena, Dimitrijević, Ivan, Krivokapić, Zoran, Radojković, Dragica, "Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer" in Journal of Buon, 22, no. 1 (2017):178-183,
https://hdl.handle.net/21.15107/rcub_imagine_1036 .
3