TY  - JOUR
AU  - Balint, Vanda
AU  - Perić, Mina
AU  - Dačić, Sanja
AU  - Stanisavljević Ninković, Danijela
AU  - Marjanović, Jelena
AU  - Popović, Jelena
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2340
AB  - Astrocytes are the main homeostatic cells in the central nervous system, with the unique ability to transform from quiescent into a reactive state in response to pathological conditions by reacquiring some precursor properties. This process is known as reactive astrogliosis, a compensatory response that mediates tissue damage and recovery. Although it is well known that SOX transcription factors drive the expression of phenotype-specific genetic programs during neurodevelopment, their roles in mature astrocytes have not been studied extensively. We focused on the transcription factors SOX2 and SOX9, shown to be re-expressed in reactive astrocytes, in order to study the reactivation-related functional properties of astrocytes mediated by those proteins. We performed an initial screening of SOX2 and SOX9 expression after sensorimotor cortex ablation injury in rats and conducted gain-of-function studies in vitro using astrocytes derived from the human NT2/D1 cell line. Our results revealed the direct involvement of SOX2 in the reacquisition of proliferation in mature NT2/D1-derived astrocytes, while SOX9 overexpression increased migratory potential and glutamate uptake in these cells. Our results imply that modulation of SOX gene expression may change the functional properties of astrocytes, which holds promise for the discovery of potential therapeutic targets in the development of novel strategies for tissue regeneration and recovery.
PB  - MDPI
T2  - Biomedicines
T1  - The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes
IS  - 4
SP  - 796
VL  - 12
DO  - 10.3390/biomedicines12040796
ER  - 

@article{
author = "Balint, Vanda and Perić, Mina and Dačić, Sanja and Stanisavljević Ninković, Danijela and Marjanović, Jelena and Popović, Jelena and Stevanović, Milena and Lazić, Andrijana",
year = "2024",
abstract = "Astrocytes are the main homeostatic cells in the central nervous system, with the unique ability to transform from quiescent into a reactive state in response to pathological conditions by reacquiring some precursor properties. This process is known as reactive astrogliosis, a compensatory response that mediates tissue damage and recovery. Although it is well known that SOX transcription factors drive the expression of phenotype-specific genetic programs during neurodevelopment, their roles in mature astrocytes have not been studied extensively. We focused on the transcription factors SOX2 and SOX9, shown to be re-expressed in reactive astrocytes, in order to study the reactivation-related functional properties of astrocytes mediated by those proteins. We performed an initial screening of SOX2 and SOX9 expression after sensorimotor cortex ablation injury in rats and conducted gain-of-function studies in vitro using astrocytes derived from the human NT2/D1 cell line. Our results revealed the direct involvement of SOX2 in the reacquisition of proliferation in mature NT2/D1-derived astrocytes, while SOX9 overexpression increased migratory potential and glutamate uptake in these cells. Our results imply that modulation of SOX gene expression may change the functional properties of astrocytes, which holds promise for the discovery of potential therapeutic targets in the development of novel strategies for tissue regeneration and recovery.",
publisher = "MDPI",
journal = "Biomedicines",
title = "The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes",
number = "4",
pages = "796",
volume = "12",
doi = "10.3390/biomedicines12040796"
}

Balint, V., Perić, M., Dačić, S., Stanisavljević Ninković, D., Marjanović, J., Popović, J., Stevanović, M.,& Lazić, A.. (2024). The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes. in Biomedicines
MDPI., 12(4), 796.
https://doi.org/10.3390/biomedicines12040796

Balint V, Perić M, Dačić S, Stanisavljević Ninković D, Marjanović J, Popović J, Stevanović M, Lazić A. The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes. in Biomedicines. 2024;12(4):796.
doi:10.3390/biomedicines12040796 .

Balint, Vanda, Perić, Mina, Dačić, Sanja, Stanisavljević Ninković, Danijela, Marjanović, Jelena, Popović, Jelena, Stevanović, Milena, Lazić, Andrijana, "The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes" in Biomedicines, 12, no. 4 (2024):796,
https://doi.org/10.3390/biomedicines12040796 . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Milivojević, Milena
AU  - Arsenijević, Ana
AU  - Lazić, Andrijana
AU  - Kovačević Grujičić, Nataša
AU  - Schwirtlich, Marija
AU  - Popović, Jelena
AU  - Stevanović, Milena
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1505
AB  - Genetic and molecular heterogeneity, together with intrinsic and acquired resistance to therapy, represent the major obstacles to the successful treatment of different types of breast carcinoma. Increasing evidence demonstrates that SOX transcription factors in breast carcinomas could act both as oncogenes and tumor suppressors and have been associated with tumor stage and grade, poor prognosis, and therapy resistance. Both SOX2 and SOX18 overexpression has been correlated with poor prognosis in breast carcinomas, and these genes are recognized as potential antitumor targets. Our aim was to evaluate the effect of retinoic acid (RA), a well-known cyto-differentiating agent, on breast carcinoma cells in vitro and to investigate the potential of RA treatment to modify the expression of SOX2 and SOX18 genes. By applying various experimental approaches, we evaluated the effect of RA on basic cellular processes in SK-BR-3 and MCF7 breast carcinoma cell lines. We have shown that RA inhibits cell growth, reduces the number of Ki-67 positive cells, and causes cell-cycle arrest. RA effect was more prominent in SK-BR-3 cell line that lacks SOX2 expression, including a higher decrease in cell viability, reduction in colony formation, and significant remodeling of cellular structure. We have shown that RA treatment led to the downregulation of SOX2 expression in MCF7 cells and to the reduction of SOX18 expression in both cell lines. By functional analysis, we showed that the anti-proliferative effect of RA in both cell lines was not based on the activity of stemness marker SOX2, pointing to a SOX2-independent mechanism of action. The ability of RA to reduce SOX2/SOX18 expression raises the possibility that these genes can be used as biomarkers to distinguish RA-responders from non-responders. Together, our study shows that the response of breast carcinoma cell lines to RA treatment may vary, highlighting that the development of RA-based therapy should consider differences in breast carcinoma subtypes.
PB  - Tech Science Press, Henderson
T2  - Biocell
T1  - Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells
EP  - 1367
IS  - 5
SP  - 1355
VL  - 45
DO  - 10.32604/biocell.2021.015817
ER  - 

@article{
author = "Petrović, Isidora and Milivojević, Milena and Arsenijević, Ana and Lazić, Andrijana and Kovačević Grujičić, Nataša and Schwirtlich, Marija and Popović, Jelena and Stevanović, Milena",
year = "2021",
abstract = "Genetic and molecular heterogeneity, together with intrinsic and acquired resistance to therapy, represent the major obstacles to the successful treatment of different types of breast carcinoma. Increasing evidence demonstrates that SOX transcription factors in breast carcinomas could act both as oncogenes and tumor suppressors and have been associated with tumor stage and grade, poor prognosis, and therapy resistance. Both SOX2 and SOX18 overexpression has been correlated with poor prognosis in breast carcinomas, and these genes are recognized as potential antitumor targets. Our aim was to evaluate the effect of retinoic acid (RA), a well-known cyto-differentiating agent, on breast carcinoma cells in vitro and to investigate the potential of RA treatment to modify the expression of SOX2 and SOX18 genes. By applying various experimental approaches, we evaluated the effect of RA on basic cellular processes in SK-BR-3 and MCF7 breast carcinoma cell lines. We have shown that RA inhibits cell growth, reduces the number of Ki-67 positive cells, and causes cell-cycle arrest. RA effect was more prominent in SK-BR-3 cell line that lacks SOX2 expression, including a higher decrease in cell viability, reduction in colony formation, and significant remodeling of cellular structure. We have shown that RA treatment led to the downregulation of SOX2 expression in MCF7 cells and to the reduction of SOX18 expression in both cell lines. By functional analysis, we showed that the anti-proliferative effect of RA in both cell lines was not based on the activity of stemness marker SOX2, pointing to a SOX2-independent mechanism of action. The ability of RA to reduce SOX2/SOX18 expression raises the possibility that these genes can be used as biomarkers to distinguish RA-responders from non-responders. Together, our study shows that the response of breast carcinoma cell lines to RA treatment may vary, highlighting that the development of RA-based therapy should consider differences in breast carcinoma subtypes.",
publisher = "Tech Science Press, Henderson",
journal = "Biocell",
title = "Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells",
pages = "1367-1355",
number = "5",
volume = "45",
doi = "10.32604/biocell.2021.015817"
}

Petrović, I., Milivojević, M., Arsenijević, A., Lazić, A., Kovačević Grujičić, N., Schwirtlich, M., Popović, J.,& Stevanović, M.. (2021). Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells. in Biocell
Tech Science Press, Henderson., 45(5), 1355-1367.
https://doi.org/10.32604/biocell.2021.015817

Petrović I, Milivojević M, Arsenijević A, Lazić A, Kovačević Grujičić N, Schwirtlich M, Popović J, Stevanović M. Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells. in Biocell. 2021;45(5):1355-1367.
doi:10.32604/biocell.2021.015817 .

Petrović, Isidora, Milivojević, Milena, Arsenijević, Ana, Lazić, Andrijana, Kovačević Grujičić, Nataša, Schwirtlich, Marija, Popović, Jelena, Stevanović, Milena, "Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells" in Biocell, 45, no. 5 (2021):1355-1367,
https://doi.org/10.32604/biocell.2021.015817 . .

TY  - JOUR
AU  - Lazić, Andrijana
AU  - Popović, Jelena
AU  - Paunesku, Tatjana
AU  - Woloschak, Gayle E.
AU  - Stevanović, Milena
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1385
AB  - Cancer is a global health problem that is often successfully addressed by therapy, with cancer survivors increasing in numbers and living longer world around. Although new cancer treatment options are continuously explored, platinum based chemotherapy agents remain in use due to their efficiency and availability. Unfortunately, all cancer therapies affect normal tissues as well as cancer, and more than 40 specific side effects of platinum based drugs documented so far decrease the quality of life of cancer survivors. Chemotherapy-induced peripheral neuropathy is a frequent side effects of platinum-based chemotherapy agents. This cluster of complications is often so debilitating that patients occasionally have to discontinue the therapy. Sensory neurons of dorsal root ganglia are at the core of chemotherapy-induced peripheral neuropathy symptoms. In these postmitotic cells, DNA damage caused by platinum chemotherapy interferes with normal functioning. Accumulation of DNA-platinum adducts correlates with neurotoxic severity and development of sensation of pain. While biochemistry of DNA-platinum adducts is the same in all cell types, molecular mechanisms affected by DNA-platinum adducts are different in cancer cells and non-dividing cells. This review aims to raise awareness about platinum associated chemotherapy-induced peripheral neuropathy as a medical problem that has remained unexplained for decades. We emphasize the complexity of this condition both from clinical and mechanistical point of view and focus on recent findings about chemotherapy-induced peripheral neuropathy in in vitro and in vivo model systems. Finally, we summarize current perspectives about clinical approaches for chemotherapy-induced peripheral neuropathy treatment.
PB  - Wolters Kluwer Medknow Publications, Mumbai
T2  - Neural Regeneration Research
T1  - Insights into platinum-induced peripheral neuropathy-current perspective
EP  - 1630
IS  - 9
SP  - 1623
VL  - 15
DO  - 10.4103/1673-5374.276321
ER  - 

@article{
author = "Lazić, Andrijana and Popović, Jelena and Paunesku, Tatjana and Woloschak, Gayle E. and Stevanović, Milena",
year = "2020",
abstract = "Cancer is a global health problem that is often successfully addressed by therapy, with cancer survivors increasing in numbers and living longer world around. Although new cancer treatment options are continuously explored, platinum based chemotherapy agents remain in use due to their efficiency and availability. Unfortunately, all cancer therapies affect normal tissues as well as cancer, and more than 40 specific side effects of platinum based drugs documented so far decrease the quality of life of cancer survivors. Chemotherapy-induced peripheral neuropathy is a frequent side effects of platinum-based chemotherapy agents. This cluster of complications is often so debilitating that patients occasionally have to discontinue the therapy. Sensory neurons of dorsal root ganglia are at the core of chemotherapy-induced peripheral neuropathy symptoms. In these postmitotic cells, DNA damage caused by platinum chemotherapy interferes with normal functioning. Accumulation of DNA-platinum adducts correlates with neurotoxic severity and development of sensation of pain. While biochemistry of DNA-platinum adducts is the same in all cell types, molecular mechanisms affected by DNA-platinum adducts are different in cancer cells and non-dividing cells. This review aims to raise awareness about platinum associated chemotherapy-induced peripheral neuropathy as a medical problem that has remained unexplained for decades. We emphasize the complexity of this condition both from clinical and mechanistical point of view and focus on recent findings about chemotherapy-induced peripheral neuropathy in in vitro and in vivo model systems. Finally, we summarize current perspectives about clinical approaches for chemotherapy-induced peripheral neuropathy treatment.",
publisher = "Wolters Kluwer Medknow Publications, Mumbai",
journal = "Neural Regeneration Research",
title = "Insights into platinum-induced peripheral neuropathy-current perspective",
pages = "1630-1623",
number = "9",
volume = "15",
doi = "10.4103/1673-5374.276321"
}

Lazić, A., Popović, J., Paunesku, T., Woloschak, G. E.,& Stevanović, M.. (2020). Insights into platinum-induced peripheral neuropathy-current perspective. in Neural Regeneration Research
Wolters Kluwer Medknow Publications, Mumbai., 15(9), 1623-1630.
https://doi.org/10.4103/1673-5374.276321

Lazić A, Popović J, Paunesku T, Woloschak GE, Stevanović M. Insights into platinum-induced peripheral neuropathy-current perspective. in Neural Regeneration Research. 2020;15(9):1623-1630.
doi:10.4103/1673-5374.276321 .

Lazić, Andrijana, Popović, Jelena, Paunesku, Tatjana, Woloschak, Gayle E., Stevanović, Milena, "Insights into platinum-induced peripheral neuropathy-current perspective" in Neural Regeneration Research, 15, no. 9 (2020):1623-1630,
https://doi.org/10.4103/1673-5374.276321 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Davidović, Slobodan
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1629
AB  - Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Radiation Biology
T1  - Radiation effects on early phase of NT2/D1 neural differentiation in vitro
EP  - 1639
IS  - 12
SP  - 1627
VL  - 95
DO  - 10.1080/09553002.2019.1665207
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Popović, Jelena and Petrović, Isidora and Davidović, Slobodan and Atkinson, Michael J. and Anastasov, Nataša and Stevanović, Milena",
year = "2019",
abstract = "Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Radiation Biology",
title = "Radiation effects on early phase of NT2/D1 neural differentiation in vitro",
pages = "1639-1627",
number = "12",
volume = "95",
doi = "10.1080/09553002.2019.1665207"
}

Stanisavljević Ninković, D., Popović, J., Petrović, I., Davidović, S., Atkinson, M. J., Anastasov, N.,& Stevanović, M.. (2019). Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology
Taylor & Francis Ltd, Abingdon., 95(12), 1627-1639.
https://doi.org/10.1080/09553002.2019.1665207

Stanisavljević Ninković D, Popović J, Petrović I, Davidović S, Atkinson MJ, Anastasov N, Stevanović M. Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology. 2019;95(12):1627-1639.
doi:10.1080/09553002.2019.1665207 .

Stanisavljević Ninković, Danijela, Popović, Jelena, Petrović, Isidora, Davidović, Slobodan, Atkinson, Michael J., Anastasov, Nataša, Stevanović, Milena, "Radiation effects on early phase of NT2/D1 neural differentiation in vitro" in International Journal of Radiation Biology, 95, no. 12 (2019):1627-1639,
https://doi.org/10.1080/09553002.2019.1665207 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Lazić, Andrijana
AU  - Paunesku, Tatjana
AU  - Ma, Qing
AU  - Chen, Si
AU  - Lai, Barry
AU  - Stevanović, Milena
AU  - Woloschak, Gayle E.
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1255
AB  - Chemotherapy-induced peripheral neuropathy (CIPN) is a side effect of platinum-based chemotherapy and decreases the quality of life of cancer patients. We compared neuroprotective properties of several agents using an in vitro model of terminally differentiated human cells NT2-N derived from cell line NT2/D1. Sodium azide and an active metabolite of amifostine (WR1065) increase cell viability in simultaneous treatment with cisplatin. In addition, WR1065 protects the non-dividing neurons by decreasing cisplatin caused oxidative stress and apoptosis. Accumulation of Pt in cisplatin-treated cells was heterogeneous, but the frequency and concentration of Pt in cells were lowered in the presence of WR1065 as shown by X-ray fluorescence microscopy (XFM). Transition metals accumulation accompanied Pt increase in cells; this effect was equally diminished in the presence of WR1065. To analyze possible chemical modulation of Pt-DNA bonds, we examined the platinum L-III near edge spectrum by X-ray absorption spectroscopy. The spectrum found in cisplatin-DNA samples is altered differently by the addition of either WR1065 or sodium azide. Importantly, a similar change in Pt edge spectra was noted in cells treated with cisplatin and WR1065. Therefore, amifostine should be reconsidered as a candidate for treatments that reduce or prevent CIPN.
PB  - Springer/Plenum Publishers, New York
T2  - Cellular and Molecular Neurobiology
T1  - Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro
EP  - 636
IS  - 5
SP  - 619
VL  - 39
DO  - 10.1007/s10571-019-00667-7
ER  - 

@article{
author = "Popović, Jelena and Lazić, Andrijana and Paunesku, Tatjana and Ma, Qing and Chen, Si and Lai, Barry and Stevanović, Milena and Woloschak, Gayle E.",
year = "2019",
abstract = "Chemotherapy-induced peripheral neuropathy (CIPN) is a side effect of platinum-based chemotherapy and decreases the quality of life of cancer patients. We compared neuroprotective properties of several agents using an in vitro model of terminally differentiated human cells NT2-N derived from cell line NT2/D1. Sodium azide and an active metabolite of amifostine (WR1065) increase cell viability in simultaneous treatment with cisplatin. In addition, WR1065 protects the non-dividing neurons by decreasing cisplatin caused oxidative stress and apoptosis. Accumulation of Pt in cisplatin-treated cells was heterogeneous, but the frequency and concentration of Pt in cells were lowered in the presence of WR1065 as shown by X-ray fluorescence microscopy (XFM). Transition metals accumulation accompanied Pt increase in cells; this effect was equally diminished in the presence of WR1065. To analyze possible chemical modulation of Pt-DNA bonds, we examined the platinum L-III near edge spectrum by X-ray absorption spectroscopy. The spectrum found in cisplatin-DNA samples is altered differently by the addition of either WR1065 or sodium azide. Importantly, a similar change in Pt edge spectra was noted in cells treated with cisplatin and WR1065. Therefore, amifostine should be reconsidered as a candidate for treatments that reduce or prevent CIPN.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Cellular and Molecular Neurobiology",
title = "Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro",
pages = "636-619",
number = "5",
volume = "39",
doi = "10.1007/s10571-019-00667-7"
}

Popović, J., Lazić, A., Paunesku, T., Ma, Q., Chen, S., Lai, B., Stevanović, M.,& Woloschak, G. E.. (2019). Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro. in Cellular and Molecular Neurobiology
Springer/Plenum Publishers, New York., 39(5), 619-636.
https://doi.org/10.1007/s10571-019-00667-7

Popović J, Lazić A, Paunesku T, Ma Q, Chen S, Lai B, Stevanović M, Woloschak GE. Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro. in Cellular and Molecular Neurobiology. 2019;39(5):619-636.
doi:10.1007/s10571-019-00667-7 .

Popović, Jelena, Lazić, Andrijana, Paunesku, Tatjana, Ma, Qing, Chen, Si, Lai, Barry, Stevanović, Milena, Woloschak, Gayle E., "Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro" in Cellular and Molecular Neurobiology, 39, no. 5 (2019):619-636,
https://doi.org/10.1007/s10571-019-00667-7 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Davidović, Slobodan
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1206
AB  - Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Radiation Biology
T1  - Radiation effects on early phase of NT2/D1 neural differentiation in vitro
EP  - 1639
IS  - 12
SP  - 1627
VL  - 95
DO  - 10.1080/09553002.2019.1665207
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Popović, Jelena and Petrović, Isidora and Davidović, Slobodan and Atkinson, Michael J. and Anastasov, Nataša and Stevanović, Milena",
year = "2019",
abstract = "Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Radiation Biology",
title = "Radiation effects on early phase of NT2/D1 neural differentiation in vitro",
pages = "1639-1627",
number = "12",
volume = "95",
doi = "10.1080/09553002.2019.1665207"
}

Stanisavljević Ninković, D., Popović, J., Petrović, I., Davidović, S., Atkinson, M. J., Anastasov, N.,& Stevanović, M.. (2019). Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology
Taylor & Francis Ltd, Abingdon., 95(12), 1627-1639.
https://doi.org/10.1080/09553002.2019.1665207

Stanisavljević Ninković D, Popović J, Petrović I, Davidović S, Atkinson MJ, Anastasov N, Stevanović M. Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology. 2019;95(12):1627-1639.
doi:10.1080/09553002.2019.1665207 .

Stanisavljević Ninković, Danijela, Popović, Jelena, Petrović, Isidora, Davidović, Slobodan, Atkinson, Michael J., Anastasov, Nataša, Stevanović, Milena, "Radiation effects on early phase of NT2/D1 neural differentiation in vitro" in International Journal of Radiation Biology, 95, no. 12 (2019):1627-1639,
https://doi.org/10.1080/09553002.2019.1665207 . .

TY  - CONF
AU  - Paunesku, Tatjana
AU  - Popović, Jelena
AU  - Klajn, Andrijana
AU  - Kovačević Grujičić, Nataša
AU  - Ma, Qing
AU  - Stevanović, Milena
AU  - Woloschak, Gayle E.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1167
PB  - Amer Assoc Cancer Research, Philadelphia
C3  - Cancer Research
T1  - WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin
IS  - 13
VL  - 78
DO  - 10.1158/1538-7445.AM2018-456
ER  - 

@conference{
author = "Paunesku, Tatjana and Popović, Jelena and Klajn, Andrijana and Kovačević Grujičić, Nataša and Ma, Qing and Stevanović, Milena and Woloschak, Gayle E.",
year = "2018",
publisher = "Amer Assoc Cancer Research, Philadelphia",
journal = "Cancer Research",
title = "WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin",
number = "13",
volume = "78",
doi = "10.1158/1538-7445.AM2018-456"
}

Paunesku, T., Popović, J., Klajn, A., Kovačević Grujičić, N., Ma, Q., Stevanović, M.,& Woloschak, G. E.. (2018). WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin. in Cancer Research
Amer Assoc Cancer Research, Philadelphia., 78(13).
https://doi.org/10.1158/1538-7445.AM2018-456

Paunesku T, Popović J, Klajn A, Kovačević Grujičić N, Ma Q, Stevanović M, Woloschak GE. WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin. in Cancer Research. 2018;78(13).
doi:10.1158/1538-7445.AM2018-456 .

Paunesku, Tatjana, Popović, Jelena, Klajn, Andrijana, Kovačević Grujičić, Nataša, Ma, Qing, Stevanović, Milena, Woloschak, Gayle E., "WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin" in Cancer Research, 78, no. 13 (2018),
https://doi.org/10.1158/1538-7445.AM2018-456 . .

TY  - JOUR
AU  - Stojković, Dejan S.
AU  - Kovačević Grujičić, Nataša
AU  - Reis, Filipa S.
AU  - Davidović, Slobodan
AU  - Barros, Lillian
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Pavić, Aleksandar
AU  - Glamoclija, Jasmina
AU  - Cirić, Ana
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1051
AB  - Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with delta- and gamma-tocopherols being predominant (123.35 and 77.80 mu g/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 mu g/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125-5 mg/mL; MIC/MFC 0.025-0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (lC(50) 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.
PB  - Elsevier Science Bv, Amsterdam
T2  - Lwt-Food Science and Technology
T1  - Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract
EP  - 462
SP  - 454
VL  - 79
DO  - 10.1016/j.lwt.2017.01.045
ER  - 

@article{
author = "Stojković, Dejan S. and Kovačević Grujičić, Nataša and Reis, Filipa S. and Davidović, Slobodan and Barros, Lillian and Popović, Jelena and Petrović, Isidora and Pavić, Aleksandar and Glamoclija, Jasmina and Cirić, Ana and Stevanović, Milena and Ferreira, Isabel C. F. R. and Soković, Marina",
year = "2017",
abstract = "Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with delta- and gamma-tocopherols being predominant (123.35 and 77.80 mu g/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 mu g/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125-5 mg/mL; MIC/MFC 0.025-0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (lC(50) 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Lwt-Food Science and Technology",
title = "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract",
pages = "462-454",
volume = "79",
doi = "10.1016/j.lwt.2017.01.045"
}

Stojković, D. S., Kovačević Grujičić, N., Reis, F. S., Davidović, S., Barros, L., Popović, J., Petrović, I., Pavić, A., Glamoclija, J., Cirić, A., Stevanović, M., Ferreira, I. C. F. R.,& Soković, M.. (2017). Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in Lwt-Food Science and Technology
Elsevier Science Bv, Amsterdam., 79, 454-462.
https://doi.org/10.1016/j.lwt.2017.01.045

Stojković DS, Kovačević Grujičić N, Reis FS, Davidović S, Barros L, Popović J, Petrović I, Pavić A, Glamoclija J, Cirić A, Stevanović M, Ferreira ICFR, Soković M. Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in Lwt-Food Science and Technology. 2017;79:454-462.
doi:10.1016/j.lwt.2017.01.045 .

Stojković, Dejan S., Kovačević Grujičić, Nataša, Reis, Filipa S., Davidović, Slobodan, Barros, Lillian, Popović, Jelena, Petrović, Isidora, Pavić, Aleksandar, Glamoclija, Jasmina, Cirić, Ana, Stevanović, Milena, Ferreira, Isabel C. F. R., Soković, Marina, "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract" in Lwt-Food Science and Technology, 79 (2017):454-462,
https://doi.org/10.1016/j.lwt.2017.01.045 . .

TY  - JOUR
AU  - Smiljković, Marija
AU  - Stanisavljević Ninković, Danijela
AU  - Stojković, Dejan
AU  - Petrović, Isidora
AU  - Vicentić, Jelena Marjanovic
AU  - Popović, Jelena
AU  - Grdadolnik, Simona Golic
AU  - Marković, Dejan
AU  - Sanković-Babić, Snežana
AU  - Glamoclija, Jasmina
AU  - Stevanović, Milena
AU  - Soković, Marina
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1014
AB  - Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra-and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.
PB  - EXCLI Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions
EP  - 807
SP  - 795
VL  - 16
DO  - 10.17179/excli2017-300
ER  - 

@article{
author = "Smiljković, Marija and Stanisavljević Ninković, Danijela and Stojković, Dejan and Petrović, Isidora and Vicentić, Jelena Marjanovic and Popović, Jelena and Grdadolnik, Simona Golic and Marković, Dejan and Sanković-Babić, Snežana and Glamoclija, Jasmina and Stevanović, Milena and Soković, Marina",
year = "2017",
abstract = "Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra-and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.",
publisher = "EXCLI Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions",
pages = "807-795",
volume = "16",
doi = "10.17179/excli2017-300"
}

Smiljković, M., Stanisavljević Ninković, D., Stojković, D., Petrović, I., Vicentić, J. M., Popović, J., Grdadolnik, S. G., Marković, D., Sanković-Babić, S., Glamoclija, J., Stevanović, M.,& Soković, M.. (2017). Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal
EXCLI Journal Managing Office, Dortmund., 16, 795-807.
https://doi.org/10.17179/excli2017-300

Smiljković M, Stanisavljević Ninković D, Stojković D, Petrović I, Vicentić JM, Popović J, Grdadolnik SG, Marković D, Sanković-Babić S, Glamoclija J, Stevanović M, Soković M. Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal. 2017;16:795-807.
doi:10.17179/excli2017-300 .

Smiljković, Marija, Stanisavljević Ninković, Danijela, Stojković, Dejan, Petrović, Isidora, Vicentić, Jelena Marjanovic, Popović, Jelena, Grdadolnik, Simona Golic, Marković, Dejan, Sanković-Babić, Snežana, Glamoclija, Jasmina, Stevanović, Milena, Soković, Marina, "Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions" in EXCLI Journal, 16 (2017):795-807,
https://doi.org/10.17179/excli2017-300 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Petrović, Isidora
AU  - Vuković, Vladanka
AU  - Schwirtlich, Marija
AU  - Gredić, Marija
AU  - Stevanović, Milena
AU  - Popović, Jelena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/996
AB  - SOX14 is a member of the SOX family of transcription factors mainly involved in the regulation of neural development. Recently, it became evident that SOX14 is one of four hyper-methylated genes in cervical carcinoma, considered as a tumor suppressor candidate in this type of malignancy. In this paper we elucidated the role of SOX14 in the regulation of malignant properties of cervical carcinoma cells in vitro. Functional analysis performed in HeLa cells revealed that SOX14 overexpression decreased viability and promoted apoptosis through altering the expression of apoptosis related genes. Our results demonstrated that overexpression of SOX14 initiated accumulation of p53, demonstrating potential cross-talk between SOX14 and the p53 signaling pathway. Further analysis unambiguously showed that SOX14 triggered posttranslational modification of p53 protein, as detected by the significantly increased level of phospho-p53 (Ser-15) in SOX14-overexpressing HeLa cells. Moreover, the obtained results revealed that SOX14 activated p53 protein, which was confirmed by elevated p21 Waf1/Cip1, a well known target gene of p53. This study advances our understanding about the role of SOX14 and might explain the molecular mechanism by which this transcription factor could exert tumor suppressor properties in cervical carcinoma.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line
IS  - 9
VL  - 12
DO  - 10.1371/journal.pone.0184686
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Petrović, Isidora and Vuković, Vladanka and Schwirtlich, Marija and Gredić, Marija and Stevanović, Milena and Popović, Jelena",
year = "2017",
abstract = "SOX14 is a member of the SOX family of transcription factors mainly involved in the regulation of neural development. Recently, it became evident that SOX14 is one of four hyper-methylated genes in cervical carcinoma, considered as a tumor suppressor candidate in this type of malignancy. In this paper we elucidated the role of SOX14 in the regulation of malignant properties of cervical carcinoma cells in vitro. Functional analysis performed in HeLa cells revealed that SOX14 overexpression decreased viability and promoted apoptosis through altering the expression of apoptosis related genes. Our results demonstrated that overexpression of SOX14 initiated accumulation of p53, demonstrating potential cross-talk between SOX14 and the p53 signaling pathway. Further analysis unambiguously showed that SOX14 triggered posttranslational modification of p53 protein, as detected by the significantly increased level of phospho-p53 (Ser-15) in SOX14-overexpressing HeLa cells. Moreover, the obtained results revealed that SOX14 activated p53 protein, which was confirmed by elevated p21 Waf1/Cip1, a well known target gene of p53. This study advances our understanding about the role of SOX14 and might explain the molecular mechanism by which this transcription factor could exert tumor suppressor properties in cervical carcinoma.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line",
number = "9",
volume = "12",
doi = "10.1371/journal.pone.0184686"
}

Stanisavljević Ninković, D., Petrović, I., Vuković, V., Schwirtlich, M., Gredić, M., Stevanović, M.,& Popović, J.. (2017). SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line. in PLoS One
Public Library Science, San Francisco., 12(9).
https://doi.org/10.1371/journal.pone.0184686

Stanisavljević Ninković D, Petrović I, Vuković V, Schwirtlich M, Gredić M, Stevanović M, Popović J. SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line. in PLoS One. 2017;12(9).
doi:10.1371/journal.pone.0184686 .

Stanisavljević Ninković, Danijela, Petrović, Isidora, Vuković, Vladanka, Schwirtlich, Marija, Gredić, Marija, Stevanović, Milena, Popović, Jelena, "SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line" in PLoS One, 12, no. 9 (2017),
https://doi.org/10.1371/journal.pone.0184686 . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Milivojević, Milena
AU  - Popović, Jelena
AU  - Schwirtlich, Marija
AU  - Ranković, Branislava
AU  - Stevanović, Milena
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/836
AB  - Although there is much evidence showing functional relationship between Hedgehog pathway, in particular Sonic hedgehog, and SOX transcription factors during embryonic development, scarce data are available regarding their crosstalk in cancer cells. SOX18 protein plays an important role in promoting tumor angiogenesis and therefore emerged as a promising potential target in antiangiogenic tumor therapy. Recently it became evident that expression of SOX18 gene in tumors is not restricted to endothelium of accompanying blood and lymphatic vessels, but in tumor cells as well. In this paper we have identified human SOX18 gene as a novel target gene of Hedgehog signaling in cervical carcinoma cell lines. We have presented data showing that expression of SOX18 gene is regulated by GLI1 and GLI2 transcription factors, final effectors of Hedgehog signaling, and that modulation of Hedgehog signaling activity in considerably influence SOX18 expression. We consider important that Hedgehog pathway inhibitors reduced SOX18 expression, thus showing, for the first time, possibility for manipulationwith SOX18 gene expression. In addition, we analyzed the role of SOX18 in malignant potential of cervical carcinoma cell line, and showed that its overexpression has no influence on cells proliferation and viability, but substantially promotes migration and invasion of cells in vitro. Pro-migratory effect of SOX18 suggests its role in promoting malignant spreading, possibly in response to Hedgehog activation.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines
IS  - 11
VL  - 10
DO  - 10.1371/journal.pone.0143591
ER  - 

@article{
author = "Petrović, Isidora and Milivojević, Milena and Popović, Jelena and Schwirtlich, Marija and Ranković, Branislava and Stevanović, Milena",
year = "2015",
abstract = "Although there is much evidence showing functional relationship between Hedgehog pathway, in particular Sonic hedgehog, and SOX transcription factors during embryonic development, scarce data are available regarding their crosstalk in cancer cells. SOX18 protein plays an important role in promoting tumor angiogenesis and therefore emerged as a promising potential target in antiangiogenic tumor therapy. Recently it became evident that expression of SOX18 gene in tumors is not restricted to endothelium of accompanying blood and lymphatic vessels, but in tumor cells as well. In this paper we have identified human SOX18 gene as a novel target gene of Hedgehog signaling in cervical carcinoma cell lines. We have presented data showing that expression of SOX18 gene is regulated by GLI1 and GLI2 transcription factors, final effectors of Hedgehog signaling, and that modulation of Hedgehog signaling activity in considerably influence SOX18 expression. We consider important that Hedgehog pathway inhibitors reduced SOX18 expression, thus showing, for the first time, possibility for manipulationwith SOX18 gene expression. In addition, we analyzed the role of SOX18 in malignant potential of cervical carcinoma cell line, and showed that its overexpression has no influence on cells proliferation and viability, but substantially promotes migration and invasion of cells in vitro. Pro-migratory effect of SOX18 suggests its role in promoting malignant spreading, possibly in response to Hedgehog activation.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines",
number = "11",
volume = "10",
doi = "10.1371/journal.pone.0143591"
}

Petrović, I., Milivojević, M., Popović, J., Schwirtlich, M., Ranković, B.,& Stevanović, M.. (2015). SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. in PLoS One
Public Library Science, San Francisco., 10(11).
https://doi.org/10.1371/journal.pone.0143591

Petrović I, Milivojević M, Popović J, Schwirtlich M, Ranković B, Stevanović M. SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. in PLoS One. 2015;10(11).
doi:10.1371/journal.pone.0143591 .

Petrović, Isidora, Milivojević, Milena, Popović, Jelena, Schwirtlich, Marija, Ranković, Branislava, Stevanović, Milena, "SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines" in PLoS One, 10, no. 11 (2015),
https://doi.org/10.1371/journal.pone.0143591 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Stanisavljević Ninković, Danijela
AU  - Schwirtlich, Marija
AU  - Lazić, Andrijana
AU  - Marjanović, Jelena
AU  - Stevanović, Milena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/785
AB  - SOX14 is a member of the SOXB2 subgroup of transcription factors implicated in neural development. Although the first SOX14 gene in vertebrates was cloned and characterized more than a decade ago and its expression profile during development was revealed in various animal model systems, the role of this gene during neural development is largely unknown. In the present study we analyzed the expression of SOX14 in human NT2/D1 and mouse P19 pluripotent embryonal carcinoma cells. We demonstrated that it is expressed in both cell lines and upregulated during retinoic acid induced neural differentiation. We showed that SOX14 was expressed in both neuronal and non-neuronal differentiated derivatives, as revealed by immunocytochemistry. Since it was previously proposed that increased SOXB2 proteins level interfere with the activity of SOXB1 counteracting partners, we compared expression patterns of SOXB members during retinoic acid induction of embryonal carcinoma cells. We revealed that upregulation of SOX14 expression is accompanied by alterations in the expression patterns of SOXB1 members. In order to analyze the potential cross-talk between them, we generated SOX14 expression construct. The ectopic expression of SOX14 was demonstrated at the mRNA level in NT2/D1, P19 and HeLa cells, while an increased level of SOX14 protein was detected in HeLa cells only. By transient transfection experiments in HeLa cells we showed for the first time that ectopic expression of SOX14 repressed SOX1 expression, whereas no significant effect on SOX2, SOX3 and SOX21 was observed. Data presented here provide an insight into SOX14 expression during in vitro neural differentiation of embryonal carcinoma cells and demonstrate the effect of its ectopic expression on protein levels of SOXB members in HeLa cells. Obtained results contribute to better understanding the role of one of the most conserved SOX proteins.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells
IS  - 3
VL  - 9
DO  - 10.1371/journal.pone.0091852
ER  - 

@article{
author = "Popović, Jelena and Stanisavljević Ninković, Danijela and Schwirtlich, Marija and Lazić, Andrijana and Marjanović, Jelena and Stevanović, Milena",
year = "2014",
abstract = "SOX14 is a member of the SOXB2 subgroup of transcription factors implicated in neural development. Although the first SOX14 gene in vertebrates was cloned and characterized more than a decade ago and its expression profile during development was revealed in various animal model systems, the role of this gene during neural development is largely unknown. In the present study we analyzed the expression of SOX14 in human NT2/D1 and mouse P19 pluripotent embryonal carcinoma cells. We demonstrated that it is expressed in both cell lines and upregulated during retinoic acid induced neural differentiation. We showed that SOX14 was expressed in both neuronal and non-neuronal differentiated derivatives, as revealed by immunocytochemistry. Since it was previously proposed that increased SOXB2 proteins level interfere with the activity of SOXB1 counteracting partners, we compared expression patterns of SOXB members during retinoic acid induction of embryonal carcinoma cells. We revealed that upregulation of SOX14 expression is accompanied by alterations in the expression patterns of SOXB1 members. In order to analyze the potential cross-talk between them, we generated SOX14 expression construct. The ectopic expression of SOX14 was demonstrated at the mRNA level in NT2/D1, P19 and HeLa cells, while an increased level of SOX14 protein was detected in HeLa cells only. By transient transfection experiments in HeLa cells we showed for the first time that ectopic expression of SOX14 repressed SOX1 expression, whereas no significant effect on SOX2, SOX3 and SOX21 was observed. Data presented here provide an insight into SOX14 expression during in vitro neural differentiation of embryonal carcinoma cells and demonstrate the effect of its ectopic expression on protein levels of SOXB members in HeLa cells. Obtained results contribute to better understanding the role of one of the most conserved SOX proteins.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells",
number = "3",
volume = "9",
doi = "10.1371/journal.pone.0091852"
}

Popović, J., Stanisavljević Ninković, D., Schwirtlich, M., Lazić, A., Marjanović, J.,& Stevanović, M.. (2014). Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells. in PLoS One
Public Library Science, San Francisco., 9(3).
https://doi.org/10.1371/journal.pone.0091852

Popović J, Stanisavljević Ninković D, Schwirtlich M, Lazić A, Marjanović J, Stevanović M. Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells. in PLoS One. 2014;9(3).
doi:10.1371/journal.pone.0091852 .

Popović, Jelena, Stanisavljević Ninković, Danijela, Schwirtlich, Marija, Lazić, Andrijana, Marjanović, Jelena, Stevanović, Milena, "Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells" in PLoS One, 9, no. 3 (2014),
https://doi.org/10.1371/journal.pone.0091852 . .

TY  - JOUR
AU  - Kovačević Grujičić, Nataša
AU  - Mojsin, Marija
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Topalović, Vladanka
AU  - Stevanović, Milena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/723
AB  - SOX3 is one of the earliest neural markers in vertebrates, playing the role in specifying neuronal fate. In this study we have established first functional link between CREB and human SOX3 gene which both have important roles in the nervous system throughout development and in the adulthood. Here we demonstrate both in vitro and in vivo that CREB binds to CRE half-site located -195 to -191 within the human SOX3 promoter. Overexpression studies with CREB or its dominant-negative inhibitor A-CREB indicate that this transcription factor acts as a positive regulator of basal SOX3 gene expression in NT2/D1 cells. This is further confirmed by mutational analysis where mutation of CREB binding site results in reduction of SOX3 promoter activity. Our results point at CREB as a positive regulator of SOX3 gene transcription in NT2/D1 cells, while its contribution to RA induction of SOX3 promoter is not prominent.
PB  - Korean Society Biochemistry & Molecular Biology, Seoul
T2  - BMB Reports
T1  - Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells
EP  - 202
IS  - 4
SP  - 197
VL  - 47
DO  - 10.5483/BMBRep.2014.47.4.084
ER  - 

@article{
author = "Kovačević Grujičić, Nataša and Mojsin, Marija and Popović, Jelena and Petrović, Isidora and Topalović, Vladanka and Stevanović, Milena",
year = "2014",
abstract = "SOX3 is one of the earliest neural markers in vertebrates, playing the role in specifying neuronal fate. In this study we have established first functional link between CREB and human SOX3 gene which both have important roles in the nervous system throughout development and in the adulthood. Here we demonstrate both in vitro and in vivo that CREB binds to CRE half-site located -195 to -191 within the human SOX3 promoter. Overexpression studies with CREB or its dominant-negative inhibitor A-CREB indicate that this transcription factor acts as a positive regulator of basal SOX3 gene expression in NT2/D1 cells. This is further confirmed by mutational analysis where mutation of CREB binding site results in reduction of SOX3 promoter activity. Our results point at CREB as a positive regulator of SOX3 gene transcription in NT2/D1 cells, while its contribution to RA induction of SOX3 promoter is not prominent.",
publisher = "Korean Society Biochemistry & Molecular Biology, Seoul",
journal = "BMB Reports",
title = "Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells",
pages = "202-197",
number = "4",
volume = "47",
doi = "10.5483/BMBRep.2014.47.4.084"
}

Kovačević Grujičić, N., Mojsin, M., Popović, J., Petrović, I., Topalović, V.,& Stevanović, M.. (2014). Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells. in BMB Reports
Korean Society Biochemistry & Molecular Biology, Seoul., 47(4), 197-202.
https://doi.org/10.5483/BMBRep.2014.47.4.084

Kovačević Grujičić N, Mojsin M, Popović J, Petrović I, Topalović V, Stevanović M. Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells. in BMB Reports. 2014;47(4):197-202.
doi:10.5483/BMBRep.2014.47.4.084 .

Kovačević Grujičić, Nataša, Mojsin, Marija, Popović, Jelena, Petrović, Isidora, Topalović, Vladanka, Stevanović, Milena, "Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells" in BMB Reports, 47, no. 4 (2014):197-202,
https://doi.org/10.5483/BMBRep.2014.47.4.084 . .

TY  - CONF
AU  - Petrović, Isidora
AU  - Popović, Jelena
AU  - Stanisavljević Ninković, Danijela
AU  - Schwirtlich, Marija
AU  - Klajn, Andrijana
AU  - Marjanović, J.
AU  - Kovačević Grujičić, Nataša
AU  - Topalović, Vladanka
AU  - Mojsin, Marija
AU  - Stevanović, Milena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/731
PB  - Elsevier Sci Ltd, Oxford
C3  - European Journal of Cancer
T1  - SOX14 downregulates SOX1 expression in HeLa cells
EP  - S56
SP  - S56
VL  - 50
DO  - 10.1016/S0959-8049(14)50210-9
ER  - 

@conference{
author = "Petrović, Isidora and Popović, Jelena and Stanisavljević Ninković, Danijela and Schwirtlich, Marija and Klajn, Andrijana and Marjanović, J. and Kovačević Grujičić, Nataša and Topalović, Vladanka and Mojsin, Marija and Stevanović, Milena",
year = "2014",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "European Journal of Cancer",
title = "SOX14 downregulates SOX1 expression in HeLa cells",
pages = "S56-S56",
volume = "50",
doi = "10.1016/S0959-8049(14)50210-9"
}

Petrović, I., Popović, J., Stanisavljević Ninković, D., Schwirtlich, M., Klajn, A., Marjanović, J., Kovačević Grujičić, N., Topalović, V., Mojsin, M.,& Stevanović, M.. (2014). SOX14 downregulates SOX1 expression in HeLa cells. in European Journal of Cancer
Elsevier Sci Ltd, Oxford., 50, S56-S56.
https://doi.org/10.1016/S0959-8049(14)50210-9

Petrović I, Popović J, Stanisavljević Ninković D, Schwirtlich M, Klajn A, Marjanović J, Kovačević Grujičić N, Topalović V, Mojsin M, Stevanović M. SOX14 downregulates SOX1 expression in HeLa cells. in European Journal of Cancer. 2014;50:S56-S56.
doi:10.1016/S0959-8049(14)50210-9 .

Petrović, Isidora, Popović, Jelena, Stanisavljević Ninković, Danijela, Schwirtlich, Marija, Klajn, Andrijana, Marjanović, J., Kovačević Grujičić, Nataša, Topalović, Vladanka, Mojsin, Marija, Stevanović, Milena, "SOX14 downregulates SOX1 expression in HeLa cells" in European Journal of Cancer, 50 (2014):S56-S56,
https://doi.org/10.1016/S0959-8049(14)50210-9 . .

TY  - JOUR
AU  - Milivojević, Milena
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Popović, Jelena
AU  - Mojsin, Marija
AU  - Stevanović, Milena
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/672
AB  - SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Biochemistry-Moscow
T1  - Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein
EP  - 1292
IS  - 11
SP  - 1287
VL  - 78
DO  - 10.1134/S0006297913110096
ER  - 

@article{
author = "Milivojević, Milena and Petrović, Isidora and Kovačević Grujičić, Nataša and Popović, Jelena and Mojsin, Marija and Stevanović, Milena",
year = "2013",
abstract = "SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Biochemistry-Moscow",
title = "Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein",
pages = "1292-1287",
number = "11",
volume = "78",
doi = "10.1134/S0006297913110096"
}

Milivojević, M., Petrović, I., Kovačević Grujičić, N., Popović, J., Mojsin, M.,& Stevanović, M.. (2013). Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein. in Biochemistry-Moscow
Maik Nauka/Interperiodica/Springer, New York., 78(11), 1287-1292.
https://doi.org/10.1134/S0006297913110096

Milivojević M, Petrović I, Kovačević Grujičić N, Popović J, Mojsin M, Stevanović M. Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein. in Biochemistry-Moscow. 2013;78(11):1287-1292.
doi:10.1134/S0006297913110096 .

Milivojević, Milena, Petrović, Isidora, Kovačević Grujičić, Nataša, Popović, Jelena, Mojsin, Marija, Stevanović, Milena, "Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein" in Biochemistry-Moscow, 78, no. 11 (2013):1287-1292,
https://doi.org/10.1134/S0006297913110096 . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Popović, Jelena
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/585
AB  - SOX3 is a member of the Sox gene family implicated in brain formation and cognitive function. It is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. Recently, we have established the first link between TALE (three-amino-acid loop extension) proteins, PBX1 (pre-B-cell leukemia homeobox 1) and MEIS1 (myeloid ecotropic viral integration site 1 homologue), and the expression of the human SOX3 gene. Here we present the evidence that TGIF (TG-interacting factor) is an additional TALE superfamily member involved in the regulation of human SOX3 gene expression in NT2/D1 cells by direct interaction with the consensus binding site that is conserved in primate orthologue promoters. Functional analysis demonstrated that mutation of the TGIF binding site resulted in the activation of SOX3 promoter. TGIF overexpression downregulates SOX3 promoter activity and decreases endogenous SOX3 protein expression in both uninduced and retinoic acid (RA)-induced NT2/D1 cells. Up to now, this is the first transcription factor identified as a negative regulator of SOX3 gene expression. The obtained results further underscore the significance of TALE proteins as important transcriptional regulators of SOX3 gene expression.
PB  - Science Press, Beijing
T2  - Journal of Genetics and Genomics
T1  - TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line
EP  - 27
IS  - 1
SP  - 19
VL  - 39
DO  - 10.1016/j.jgg.2011.11.006
ER  - 

@article{
author = "Mojsin, Marija and Popović, Jelena and Kovačević Grujičić, Nataša and Stevanović, Milena",
year = "2012",
abstract = "SOX3 is a member of the Sox gene family implicated in brain formation and cognitive function. It is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. Recently, we have established the first link between TALE (three-amino-acid loop extension) proteins, PBX1 (pre-B-cell leukemia homeobox 1) and MEIS1 (myeloid ecotropic viral integration site 1 homologue), and the expression of the human SOX3 gene. Here we present the evidence that TGIF (TG-interacting factor) is an additional TALE superfamily member involved in the regulation of human SOX3 gene expression in NT2/D1 cells by direct interaction with the consensus binding site that is conserved in primate orthologue promoters. Functional analysis demonstrated that mutation of the TGIF binding site resulted in the activation of SOX3 promoter. TGIF overexpression downregulates SOX3 promoter activity and decreases endogenous SOX3 protein expression in both uninduced and retinoic acid (RA)-induced NT2/D1 cells. Up to now, this is the first transcription factor identified as a negative regulator of SOX3 gene expression. The obtained results further underscore the significance of TALE proteins as important transcriptional regulators of SOX3 gene expression.",
publisher = "Science Press, Beijing",
journal = "Journal of Genetics and Genomics",
title = "TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line",
pages = "27-19",
number = "1",
volume = "39",
doi = "10.1016/j.jgg.2011.11.006"
}

Mojsin, M., Popović, J., Kovačević Grujičić, N.,& Stevanović, M.. (2012). TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line. in Journal of Genetics and Genomics
Science Press, Beijing., 39(1), 19-27.
https://doi.org/10.1016/j.jgg.2011.11.006

Mojsin M, Popović J, Kovačević Grujičić N, Stevanović M. TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line. in Journal of Genetics and Genomics. 2012;39(1):19-27.
doi:10.1016/j.jgg.2011.11.006 .

Mojsin, Marija, Popović, Jelena, Kovačević Grujičić, Nataša, Stevanović, Milena, "TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line" in Journal of Genetics and Genomics, 39, no. 1 (2012):19-27,
https://doi.org/10.1016/j.jgg.2011.11.006 . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Popović, Jelena
AU  - Krstić, A.
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/526
AB  - The SOX18 transcription factor plays an important role in endothelial cell specification, angiogenesis and atherogenesis. By profiling transcription factor interactions (TranSignal TM TF Protein Array) we identified several transcription factors implicated in angiogenesis that have the ability to bind to the SOX18 optimal promoter region in vitro. In this report we focused our attention on distinct transcription factors identified by the array as belonging to AP-1 and CREB/ATF protein families. In particular, we analyzed the effects of CREB, JunB, c-Jun and ATF3 on SOX18 gene expression. Functional analysis revealed that CREB acts as a repressor, while JunB, c-Jun and ATF3 act as activators of SOX18 promoter activity. Our findings indicate that a transcriptional network that includes CREB, JunB, c-Jun and ATF3 could be involved in angiogenesis-related transcriptional regulation of the SOX18 gene.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression
EP  - 525
IS  - 3
SP  - 517
VL  - 63
DO  - 10.2298/ABS1103517P
ER  - 

@article{
author = "Petrović, Isidora and Kovačević Grujičić, Nataša and Popović, Jelena and Krstić, A. and Milivojević, Milena and Stevanović, Milena",
year = "2011",
abstract = "The SOX18 transcription factor plays an important role in endothelial cell specification, angiogenesis and atherogenesis. By profiling transcription factor interactions (TranSignal TM TF Protein Array) we identified several transcription factors implicated in angiogenesis that have the ability to bind to the SOX18 optimal promoter region in vitro. In this report we focused our attention on distinct transcription factors identified by the array as belonging to AP-1 and CREB/ATF protein families. In particular, we analyzed the effects of CREB, JunB, c-Jun and ATF3 on SOX18 gene expression. Functional analysis revealed that CREB acts as a repressor, while JunB, c-Jun and ATF3 act as activators of SOX18 promoter activity. Our findings indicate that a transcriptional network that includes CREB, JunB, c-Jun and ATF3 could be involved in angiogenesis-related transcriptional regulation of the SOX18 gene.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression",
pages = "525-517",
number = "3",
volume = "63",
doi = "10.2298/ABS1103517P"
}

Petrović, I., Kovačević Grujičić, N., Popović, J., Krstić, A., Milivojević, M.,& Stevanović, M.. (2011). Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(3), 517-525.
https://doi.org/10.2298/ABS1103517P

Petrović I, Kovačević Grujičić N, Popović J, Krstić A, Milivojević M, Stevanović M. Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression. in Archives of Biological Sciences. 2011;63(3):517-525.
doi:10.2298/ABS1103517P .

Petrović, Isidora, Kovačević Grujičić, Nataša, Popović, Jelena, Krstić, A., Milivojević, Milena, Stevanović, Milena, "Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression" in Archives of Biological Sciences, 63, no. 3 (2011):517-525,
https://doi.org/10.2298/ABS1103517P . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Kovačević Grujičić, Nataša
AU  - Krstić, Aleksandar
AU  - Popović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/407
AB  - To understand more fully the structure and evolution of the SOX3 protein, we comparatively analyzed its orthologs in vertebrates. Since complex disorders are associated with human SOX3 polyalanine expansions, our investigation focused on both compositional and evolutionary analysis of various homopolymeric amino acid tracts observed in SOX3 orthologs. Our analysis revealed that the observed homopolymeric alanine, glycine, and proline tracts are mammal-specific, except for one polyglycine tract present in birds. Since it is likely that the SOX3 protein acquired additional roles in brain development in Eutheria, we might speculate that development of novel brain functions during the course of evolution was affected, at least in part, by such structural-functional changes in the SOX3 protein.
PB  - Springer/Plenum Publishers, New York
T2  - Biochemical Genetics
T1  - Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution
EP  - 623
IS  - 7-8
SP  - 612
VL  - 48
DO  - 10.1007/s10528-010-9343-2
ER  - 

@article{
author = "Mojsin, Marija and Kovačević Grujičić, Nataša and Krstić, Aleksandar and Popović, Jelena and Milivojević, Milena and Stevanović, Milena",
year = "2010",
abstract = "To understand more fully the structure and evolution of the SOX3 protein, we comparatively analyzed its orthologs in vertebrates. Since complex disorders are associated with human SOX3 polyalanine expansions, our investigation focused on both compositional and evolutionary analysis of various homopolymeric amino acid tracts observed in SOX3 orthologs. Our analysis revealed that the observed homopolymeric alanine, glycine, and proline tracts are mammal-specific, except for one polyglycine tract present in birds. Since it is likely that the SOX3 protein acquired additional roles in brain development in Eutheria, we might speculate that development of novel brain functions during the course of evolution was affected, at least in part, by such structural-functional changes in the SOX3 protein.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Biochemical Genetics",
title = "Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution",
pages = "623-612",
number = "7-8",
volume = "48",
doi = "10.1007/s10528-010-9343-2"
}

Mojsin, M., Kovačević Grujičić, N., Krstić, A., Popović, J., Milivojević, M.,& Stevanović, M.. (2010). Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution. in Biochemical Genetics
Springer/Plenum Publishers, New York., 48(7-8), 612-623.
https://doi.org/10.1007/s10528-010-9343-2

Mojsin M, Kovačević Grujičić N, Krstić A, Popović J, Milivojević M, Stevanović M. Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution. in Biochemical Genetics. 2010;48(7-8):612-623.
doi:10.1007/s10528-010-9343-2 .

Mojsin, Marija, Kovačević Grujičić, Nataša, Krstić, Aleksandar, Popović, Jelena, Milivojević, Milena, Stevanović, Milena, "Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution" in Biochemical Genetics, 48, no. 7-8 (2010):612-623,
https://doi.org/10.1007/s10528-010-9343-2 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Lazić, Andrijana
AU  - Petrović, Isidora
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/430
AB  - The expression of Sox14 gene in spinal cord explants was found to be regulated by Sonic hedgehog (SHH) in a dose-dependent manner, indicating that this signaling molecule might act as a regulator of Sox14-expressing interneuron differentiation. In the present study we identified the positive control element and provided the first evidence that FOXA2 is involved in up-regulation of SOX14 expression in HepG2 and U87MG cell lines. By functional analysis we demonstrated that mutation in FOXA2 binding site reduced the SOX14 reporter construct activity, and that FOXA2 over-expression increased endogenous SOX14 protein expression. Further, we have shown that human SOX14 expression is GLI1 dependent in U87MG cells and SHH-N dependent in U87MG and HepG2 cell lines. By applying siRNA silencing of FOXA2, we have demonstrated that upregulation of endogenous SOX14 gene expression by SHH is, at least in part, mediated by FOXA2. However, our data revealed that a positive regulatory region, containing functional FOXA2 site analyzed in this study, is not involved in mediation of SHH dependent SOX14 activation. Data presented here provide the initial insight into molecular mechanism underlying tissue and developmentally specific regulation of the SOX14 gene expression.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms
T1  - Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression
EP  - 418
IS  - 5-6
SP  - 411
VL  - 1799
DO  - 10.1016/j.bbagrm.2010.01.002
ER  - 

@article{
author = "Popović, Jelena and Lazić, Andrijana and Petrović, Isidora and Stevanović, Milena",
year = "2010",
abstract = "The expression of Sox14 gene in spinal cord explants was found to be regulated by Sonic hedgehog (SHH) in a dose-dependent manner, indicating that this signaling molecule might act as a regulator of Sox14-expressing interneuron differentiation. In the present study we identified the positive control element and provided the first evidence that FOXA2 is involved in up-regulation of SOX14 expression in HepG2 and U87MG cell lines. By functional analysis we demonstrated that mutation in FOXA2 binding site reduced the SOX14 reporter construct activity, and that FOXA2 over-expression increased endogenous SOX14 protein expression. Further, we have shown that human SOX14 expression is GLI1 dependent in U87MG cells and SHH-N dependent in U87MG and HepG2 cell lines. By applying siRNA silencing of FOXA2, we have demonstrated that upregulation of endogenous SOX14 gene expression by SHH is, at least in part, mediated by FOXA2. However, our data revealed that a positive regulatory region, containing functional FOXA2 site analyzed in this study, is not involved in mediation of SHH dependent SOX14 activation. Data presented here provide the initial insight into molecular mechanism underlying tissue and developmentally specific regulation of the SOX14 gene expression.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms",
title = "Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression",
pages = "418-411",
number = "5-6",
volume = "1799",
doi = "10.1016/j.bbagrm.2010.01.002"
}

Popović, J., Lazić, A., Petrović, I.,& Stevanović, M.. (2010). Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression. in Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms
Elsevier Science Bv, Amsterdam., 1799(5-6), 411-418.
https://doi.org/10.1016/j.bbagrm.2010.01.002

Popović J, Lazić A, Petrović I, Stevanović M. Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression. in Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms. 2010;1799(5-6):411-418.
doi:10.1016/j.bbagrm.2010.01.002 .

Popović, Jelena, Lazić, Andrijana, Petrović, Isidora, Stevanović, Milena, "Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression" in Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms, 1799, no. 5-6 (2010):411-418,
https://doi.org/10.1016/j.bbagrm.2010.01.002 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Stevanović, Milena
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/368
AB  - SOX proteins constitute a large family of diverse, well-conserved transcription factors present in vertebrates and invertebrates, and also implicated in control of many developmental processes. Our objectives have been to identify Sox14 gene of goat (Capra hircus), cow (Bos taurus) and rat (Rattus norvegicus), and to perform comparative analyses and mapping of SOX14 orthologues from numerous vertebrate species. PCR based approach was used to identify Sox14 of goat, cow and rat, while nucleotide and amino acid sequence alignments and mapping were performed using information currently available in public database. Comparative sequence analysis revealed remarkable identity among Sox14 orthologues and helped us to identify highly conserved motifs that represent molecular signatures of SOX14 protein that might have structural or functional significance. Further, we determined chromosomal locations of numerous predicted group B Sox genes and their neighbouring genes using currently available genome database. In conclusion, our study has not only supported the proposed model of group B Sox genes evolution in chicken and mammals, but has also revealed that additional evolutionary events split Sox B genes into different chromosomes in some mammals. Mapping data presented in this study could help in refining the understanding of the evolution of group B Sox genes in vertebrates.
PB  - Indian Acad Sciences, Bangalore
T2  - Journal of Genetics
T1  - Remarkable evolutionary conservation of SOX14 orthologues
EP  - 24
IS  - 1
SP  - 15
VL  - 88
DO  - 10.1007/s12041-009-0003-4
ER  - 

@article{
author = "Popović, Jelena and Stevanović, Milena",
year = "2009",
abstract = "SOX proteins constitute a large family of diverse, well-conserved transcription factors present in vertebrates and invertebrates, and also implicated in control of many developmental processes. Our objectives have been to identify Sox14 gene of goat (Capra hircus), cow (Bos taurus) and rat (Rattus norvegicus), and to perform comparative analyses and mapping of SOX14 orthologues from numerous vertebrate species. PCR based approach was used to identify Sox14 of goat, cow and rat, while nucleotide and amino acid sequence alignments and mapping were performed using information currently available in public database. Comparative sequence analysis revealed remarkable identity among Sox14 orthologues and helped us to identify highly conserved motifs that represent molecular signatures of SOX14 protein that might have structural or functional significance. Further, we determined chromosomal locations of numerous predicted group B Sox genes and their neighbouring genes using currently available genome database. In conclusion, our study has not only supported the proposed model of group B Sox genes evolution in chicken and mammals, but has also revealed that additional evolutionary events split Sox B genes into different chromosomes in some mammals. Mapping data presented in this study could help in refining the understanding of the evolution of group B Sox genes in vertebrates.",
publisher = "Indian Acad Sciences, Bangalore",
journal = "Journal of Genetics",
title = "Remarkable evolutionary conservation of SOX14 orthologues",
pages = "24-15",
number = "1",
volume = "88",
doi = "10.1007/s12041-009-0003-4"
}

Popović, J.,& Stevanović, M.. (2009). Remarkable evolutionary conservation of SOX14 orthologues. in Journal of Genetics
Indian Acad Sciences, Bangalore., 88(1), 15-24.
https://doi.org/10.1007/s12041-009-0003-4

Popović J, Stevanović M. Remarkable evolutionary conservation of SOX14 orthologues. in Journal of Genetics. 2009;88(1):15-24.
doi:10.1007/s12041-009-0003-4 .

Popović, Jelena, Stevanović, Milena, "Remarkable evolutionary conservation of SOX14 orthologues" in Journal of Genetics, 88, no. 1 (2009):15-24,
https://doi.org/10.1007/s12041-009-0003-4 . .