TY  - CONF
AU  - Marjanović, Jelena
AU  - Drakulić, Danijela
AU  - Garcia, Idoia
AU  - Vuković, Vladanka
AU  - Aldaz, Paula
AU  - Garros-Regulez, Laura
AU  - Sampron, Nicolas
AU  - Matheu, Ander
AU  - Stevanović, Milena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1550
PB  - Springernature, London
C3  - European Journal of Human Genetics
T1  - SOX3 function in glioblastoma cells
EP  - 429
IS  - SUPPL 1
SP  - 428
VL  - 30
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1550
ER  - 

@conference{
author = "Marjanović, Jelena and Drakulić, Danijela and Garcia, Idoia and Vuković, Vladanka and Aldaz, Paula and Garros-Regulez, Laura and Sampron, Nicolas and Matheu, Ander and Stevanović, Milena",
year = "2022",
publisher = "Springernature, London",
journal = "European Journal of Human Genetics",
title = "SOX3 function in glioblastoma cells",
pages = "429-428",
number = "SUPPL 1",
volume = "30",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1550"
}

Marjanović, J., Drakulić, D., Garcia, I., Vuković, V., Aldaz, P., Garros-Regulez, L., Sampron, N., Matheu, A.,& Stevanović, M.. (2022). SOX3 function in glioblastoma cells. in European Journal of Human Genetics
Springernature, London., 30(SUPPL 1), 428-429.
https://hdl.handle.net/21.15107/rcub_imagine_1550

Marjanović J, Drakulić D, Garcia I, Vuković V, Aldaz P, Garros-Regulez L, Sampron N, Matheu A, Stevanović M. SOX3 function in glioblastoma cells. in European Journal of Human Genetics. 2022;30(SUPPL 1):428-429.
https://hdl.handle.net/21.15107/rcub_imagine_1550 .

Marjanović, Jelena, Drakulić, Danijela, Garcia, Idoia, Vuković, Vladanka, Aldaz, Paula, Garros-Regulez, Laura, Sampron, Nicolas, Matheu, Ander, Stevanović, Milena, "SOX3 function in glioblastoma cells" in European Journal of Human Genetics, 30, no. SUPPL 1 (2022):428-429,
https://hdl.handle.net/21.15107/rcub_imagine_1550 .

TY  - JOUR
AU  - Vicentić, Jelena Marjanovic
AU  - Drakulić, Danijela
AU  - Garcia, Idoia
AU  - Vuković, Vladanka
AU  - Aldaz, Paula
AU  - Puskas, Nela
AU  - Nikolić, Igor
AU  - Tasić, Goran
AU  - Raicević, Savo
AU  - Garros-Regulez, Laura
AU  - Sampron, Nicolas
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Matheu, Ander
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1285
AB  - PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.
PB  - Springer, Dordrecht
T2  - Cellular Oncology
T1  - SOX3 can promote the malignant behavior of glioblastoma cells
EP  - 54
IS  - 1
SP  - 41
VL  - 42
DO  - 10.1007/s13402-018-0405-5
ER  - 

@article{
author = "Vicentić, Jelena Marjanovic and Drakulić, Danijela and Garcia, Idoia and Vuković, Vladanka and Aldaz, Paula and Puskas, Nela and Nikolić, Igor and Tasić, Goran and Raicević, Savo and Garros-Regulez, Laura and Sampron, Nicolas and Atkinson, Michael J. and Anastasov, Nataša and Matheu, Ander and Stevanović, Milena",
year = "2019",
abstract = "PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.",
publisher = "Springer, Dordrecht",
journal = "Cellular Oncology",
title = "SOX3 can promote the malignant behavior of glioblastoma cells",
pages = "54-41",
number = "1",
volume = "42",
doi = "10.1007/s13402-018-0405-5"
}

Vicentić, J. M., Drakulić, D., Garcia, I., Vuković, V., Aldaz, P., Puskas, N., Nikolić, I., Tasić, G., Raicević, S., Garros-Regulez, L., Sampron, N., Atkinson, M. J., Anastasov, N., Matheu, A.,& Stevanović, M.. (2019). SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology
Springer, Dordrecht., 42(1), 41-54.
https://doi.org/10.1007/s13402-018-0405-5

Vicentić JM, Drakulić D, Garcia I, Vuković V, Aldaz P, Puskas N, Nikolić I, Tasić G, Raicević S, Garros-Regulez L, Sampron N, Atkinson MJ, Anastasov N, Matheu A, Stevanović M. SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology. 2019;42(1):41-54.
doi:10.1007/s13402-018-0405-5 .

Vicentić, Jelena Marjanovic, Drakulić, Danijela, Garcia, Idoia, Vuković, Vladanka, Aldaz, Paula, Puskas, Nela, Nikolić, Igor, Tasić, Goran, Raicević, Savo, Garros-Regulez, Laura, Sampron, Nicolas, Atkinson, Michael J., Anastasov, Nataša, Matheu, Ander, Stevanović, Milena, "SOX3 can promote the malignant behavior of glioblastoma cells" in Cellular Oncology, 42, no. 1 (2019):41-54,
https://doi.org/10.1007/s13402-018-0405-5 . .

TY  - JOUR
AU  - Garcia, Idoia
AU  - Aldaregia, Juncal
AU  - Vicentić, Jelena Marjanovic
AU  - Aldaz, Paula
AU  - Moreno-Cugnon, Leire
AU  - Torres-Bayona, Sergio
AU  - Carrasco-Garcia, Estefania
AU  - Garros-Regulez, Laura
AU  - Egana, Larraitz
AU  - Rubio, Angel
AU  - Pollard, Steven
AU  - Stevanović, Milena
AU  - Sampron, Nicolas
AU  - Matheu, Ander
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1083
AB  - Glioblastoma remains the most common and deadliest type of brain tumor and contains a population of self-renewing, highly tumorigenic glioma stem cells (GSCs), which contributes to tumor initiation and treatment resistance. Developmental programs participating in tissue development and homeostasis re-emerge in GSCs, supporting the development and progression of glioblastoma. SOX1 plays an important role in neural development and neural progenitor pool maintenance. Its impact on glioblastoma remains largely unknown. In this study, we have found that high levels of SOX1 observed in a subset of patients correlate with lower overall survival. At the cellular level, SOX1 expression is elevated in patient-derived GSCs and it is also higher in oncosphere culture compared to differentiation conditions in conventional glioblastoma cell lines. Moreover, genetic inhibition of SOX1 in patient-derived GSCs and conventional cell lines decreases self-renewal and proliferative capacity in vitro and tumor initiation and growth in vivo. Contrarily, SOX1 over-expression moderately promotes self-renewal and proliferation in GSCs. These functions seem to be independent of its activity as Wnt/beta-catenin signaling regulator. In summary, these results identify a functional role for SOX1 in regulating glioma cell heterogeneity and plasticity, and suggest SOX1 as a potential target in the GSC population in glioblastoma.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Oncogenic activity of SOX1 in glioblastoma
VL  - 7
DO  - 10.1038/srep46575
ER  - 

@article{
author = "Garcia, Idoia and Aldaregia, Juncal and Vicentić, Jelena Marjanovic and Aldaz, Paula and Moreno-Cugnon, Leire and Torres-Bayona, Sergio and Carrasco-Garcia, Estefania and Garros-Regulez, Laura and Egana, Larraitz and Rubio, Angel and Pollard, Steven and Stevanović, Milena and Sampron, Nicolas and Matheu, Ander",
year = "2017",
abstract = "Glioblastoma remains the most common and deadliest type of brain tumor and contains a population of self-renewing, highly tumorigenic glioma stem cells (GSCs), which contributes to tumor initiation and treatment resistance. Developmental programs participating in tissue development and homeostasis re-emerge in GSCs, supporting the development and progression of glioblastoma. SOX1 plays an important role in neural development and neural progenitor pool maintenance. Its impact on glioblastoma remains largely unknown. In this study, we have found that high levels of SOX1 observed in a subset of patients correlate with lower overall survival. At the cellular level, SOX1 expression is elevated in patient-derived GSCs and it is also higher in oncosphere culture compared to differentiation conditions in conventional glioblastoma cell lines. Moreover, genetic inhibition of SOX1 in patient-derived GSCs and conventional cell lines decreases self-renewal and proliferative capacity in vitro and tumor initiation and growth in vivo. Contrarily, SOX1 over-expression moderately promotes self-renewal and proliferation in GSCs. These functions seem to be independent of its activity as Wnt/beta-catenin signaling regulator. In summary, these results identify a functional role for SOX1 in regulating glioma cell heterogeneity and plasticity, and suggest SOX1 as a potential target in the GSC population in glioblastoma.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Oncogenic activity of SOX1 in glioblastoma",
volume = "7",
doi = "10.1038/srep46575"
}

Garcia, I., Aldaregia, J., Vicentić, J. M., Aldaz, P., Moreno-Cugnon, L., Torres-Bayona, S., Carrasco-Garcia, E., Garros-Regulez, L., Egana, L., Rubio, A., Pollard, S., Stevanović, M., Sampron, N.,& Matheu, A.. (2017). Oncogenic activity of SOX1 in glioblastoma. in Scientific Reports
Nature Publishing Group, London., 7.
https://doi.org/10.1038/srep46575

Garcia I, Aldaregia J, Vicentić JM, Aldaz P, Moreno-Cugnon L, Torres-Bayona S, Carrasco-Garcia E, Garros-Regulez L, Egana L, Rubio A, Pollard S, Stevanović M, Sampron N, Matheu A. Oncogenic activity of SOX1 in glioblastoma. in Scientific Reports. 2017;7.
doi:10.1038/srep46575 .

Garcia, Idoia, Aldaregia, Juncal, Vicentić, Jelena Marjanovic, Aldaz, Paula, Moreno-Cugnon, Leire, Torres-Bayona, Sergio, Carrasco-Garcia, Estefania, Garros-Regulez, Laura, Egana, Larraitz, Rubio, Angel, Pollard, Steven, Stevanović, Milena, Sampron, Nicolas, Matheu, Ander, "Oncogenic activity of SOX1 in glioblastoma" in Scientific Reports, 7 (2017),
https://doi.org/10.1038/srep46575 . .