TY  - CONF
AU  - Nikolić, Dragana
AU  - Divac Rankov, Aleksandra
AU  - Samardžić, Jelena
AU  - Pantelić, Ana
AU  - Spasovski, Vesna
AU  - Banović Đeri, Bojana
AU  - Kosanović, Maja
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2266
AB  - Outer membrane vesicles (OMVs), extracellular vesicles (EVs) produced by Gram-negative bacteria, are
increasingly recognised as promising tools in biomedicine due to their innate ability to interact with human
cells and trigger immune responses. The interaction of OMVs of plant growth-promoting bacteria (PGPB) with
plants, as well as with plant-pathogenic microorganisms, is far less explored. Considering the great
importance of PGPBs for the development of sustainable, environmentally friendly solutions in agriculture, the
study of the role of OMVs in PGPB-plant and PGPB-phytopathogen interactions holds valuable application
potential.
To investigate PGPB OMVs, we isolated and characterised OMVs produced by Paraburkholderia phytofirmans
PsJN, a PGPB strain known to enhance plant resistance to various abiotic and biotic stresses. After testing
different methods for isolating and purifying OMVs, a commercially available affinity-based column system
was selected as the most efficient. Outer membrane origin of isolated OMVs was confirmed using an essay for
detection of lipopolysaccharide (LPS).
To examine the interaction of OMVs with plant cells, Arabidopsis thaliana roots were incubated with isolated
P. phytofirmans PsJN vesicles, previously labelled with lipid binding fluorescent dye Vybrant™ DiD. Red signals
were observed, under confocal laser scanning microscope, in root hairs and root surface in DiD-OMV treated
plants, while in control-treated roots the same signals were missing. The results suggest direct contact of
OMVs with root hairs, which are necessary for nutrient acquisition and plant-microbe interactions in
rhizosphere. Our further research is focused on the characterization of OMV-associated RNA and its potential
delivery into host plant cells.
PB  - Serbian Society for Extracellular Vesicles (SrbEVs)
PB  - Austrian Society for Extracellular Vesicles (ASEV)
PB  - Hungarian Society for Extracellular Vesicles (HSEV)
PB  - Slovenian Network for Extracellular Vesicles (SiN-EV)
C3  - Small New World 2.0
T1  - Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots
EP  - 109
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2266
ER  - 

@conference{
author = "Nikolić, Dragana and Divac Rankov, Aleksandra and Samardžić, Jelena and Pantelić, Ana and Spasovski, Vesna and Banović Đeri, Bojana and Kosanović, Maja",
year = "2023",
abstract = "Outer membrane vesicles (OMVs), extracellular vesicles (EVs) produced by Gram-negative bacteria, are
increasingly recognised as promising tools in biomedicine due to their innate ability to interact with human
cells and trigger immune responses. The interaction of OMVs of plant growth-promoting bacteria (PGPB) with
plants, as well as with plant-pathogenic microorganisms, is far less explored. Considering the great
importance of PGPBs for the development of sustainable, environmentally friendly solutions in agriculture, the
study of the role of OMVs in PGPB-plant and PGPB-phytopathogen interactions holds valuable application
potential.
To investigate PGPB OMVs, we isolated and characterised OMVs produced by Paraburkholderia phytofirmans
PsJN, a PGPB strain known to enhance plant resistance to various abiotic and biotic stresses. After testing
different methods for isolating and purifying OMVs, a commercially available affinity-based column system
was selected as the most efficient. Outer membrane origin of isolated OMVs was confirmed using an essay for
detection of lipopolysaccharide (LPS).
To examine the interaction of OMVs with plant cells, Arabidopsis thaliana roots were incubated with isolated
P. phytofirmans PsJN vesicles, previously labelled with lipid binding fluorescent dye Vybrant™ DiD. Red signals
were observed, under confocal laser scanning microscope, in root hairs and root surface in DiD-OMV treated
plants, while in control-treated roots the same signals were missing. The results suggest direct contact of
OMVs with root hairs, which are necessary for nutrient acquisition and plant-microbe interactions in
rhizosphere. Our further research is focused on the characterization of OMV-associated RNA and its potential
delivery into host plant cells.",
publisher = "Serbian Society for Extracellular Vesicles (SrbEVs), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), Slovenian Network for Extracellular Vesicles (SiN-EV)",
journal = "Small New World 2.0",
title = "Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots",
pages = "109-109",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2266"
}

Nikolić, D., Divac Rankov, A., Samardžić, J., Pantelić, A., Spasovski, V., Banović Đeri, B.,& Kosanović, M.. (2023). Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots. in Small New World 2.0
Serbian Society for Extracellular Vesicles (SrbEVs)., 109-109.
https://hdl.handle.net/21.15107/rcub_imagine_2266

Nikolić D, Divac Rankov A, Samardžić J, Pantelić A, Spasovski V, Banović Đeri B, Kosanović M. Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots. in Small New World 2.0. 2023;:109-109.
https://hdl.handle.net/21.15107/rcub_imagine_2266 .

Nikolić, Dragana, Divac Rankov, Aleksandra, Samardžić, Jelena, Pantelić, Ana, Spasovski, Vesna, Banović Đeri, Bojana, Kosanović, Maja, "Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots" in Small New World 2.0 (2023):109-109,
https://hdl.handle.net/21.15107/rcub_imagine_2266 .

TY  - CONF
AU  - Pantelić, Ana
AU  - Prodić, Ivana
AU  - Milić, Dejana
AU  - Senćanski, Milan
AU  - Vidović, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2149
AB  - Introduction: Resurrection plants (such as Ramonda serbica) can survive a long desiccation period and
fully resume their metabolism upon watering. The hallmark of desiccation tolerance (DT) is the accumulation of protective, intrinsically disordered proteins(IDPs), called late embryogenesis abundant proteins (LEAPs). Although their high structural plasticity allows them to interact with various partners, no
specific cellular targets of LEAPs have been identified so far.
Methods: To identify LEAPsinvolved in DT, differential transcriptome and proteome analyses of hydrated
and desiccated R. serbica leaves were performed. The identified LEAPs were structurally characterised
and classified. To evaluate theirstructural propertiesin vitro and their potential functionsin vivo, the representative RsLEA proteins, were produced in Escherichia coli using recombinant DNA technology.
Results: Members of the LEA4 protein family represent the majority of desiccation-inducible LEAPs. Even
17 proteins belonging to the LEA4 protein family group were induced by desiccation. They show high disorder propensity (82 %), and at the same time, a high tendency to form α-helices (>80%). Although recombinant DNA technology has traditionally been used to overexpress and purify various globular
proteins, the production of IDPsis challenging due to their high susceptibility to proteolytic cleavage and
aggregation. Nevertheless, the representative LEAPs containing hexa-Histagsimmunoglobulin G-binding protein and a proteolytic TEV site were produced, purified and cleaved by TEV protease.
Conclusion: The combination of in silico and in vitro results will be crucial for the identification of endogenous partners of LEAPs, providing further insight into their role in DT.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica
EP  - 110
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2149
ER  - 

@conference{
author = "Pantelić, Ana and Prodić, Ivana and Milić, Dejana and Senćanski, Milan and Vidović, Marija",
year = "2023",
abstract = "Introduction: Resurrection plants (such as Ramonda serbica) can survive a long desiccation period and
fully resume their metabolism upon watering. The hallmark of desiccation tolerance (DT) is the accumulation of protective, intrinsically disordered proteins(IDPs), called late embryogenesis abundant proteins (LEAPs). Although their high structural plasticity allows them to interact with various partners, no
specific cellular targets of LEAPs have been identified so far.
Methods: To identify LEAPsinvolved in DT, differential transcriptome and proteome analyses of hydrated
and desiccated R. serbica leaves were performed. The identified LEAPs were structurally characterised
and classified. To evaluate theirstructural propertiesin vitro and their potential functionsin vivo, the representative RsLEA proteins, were produced in Escherichia coli using recombinant DNA technology.
Results: Members of the LEA4 protein family represent the majority of desiccation-inducible LEAPs. Even
17 proteins belonging to the LEA4 protein family group were induced by desiccation. They show high disorder propensity (82 %), and at the same time, a high tendency to form α-helices (>80%). Although recombinant DNA technology has traditionally been used to overexpress and purify various globular
proteins, the production of IDPsis challenging due to their high susceptibility to proteolytic cleavage and
aggregation. Nevertheless, the representative LEAPs containing hexa-Histagsimmunoglobulin G-binding protein and a proteolytic TEV site were produced, purified and cleaved by TEV protease.
Conclusion: The combination of in silico and in vitro results will be crucial for the identification of endogenous partners of LEAPs, providing further insight into their role in DT.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2149"
}

Pantelić, A., Prodić, I., Milić, D., Senćanski, M.,& Vidović, M.. (2023). Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 110-110.
https://hdl.handle.net/21.15107/rcub_imagine_2149

Pantelić A, Prodić I, Milić D, Senćanski M, Vidović M. Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:110-110.
https://hdl.handle.net/21.15107/rcub_imagine_2149 .

Pantelić, Ana, Prodić, Ivana, Milić, Dejana, Senćanski, Milan, Vidović, Marija, "Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):110-110,
https://hdl.handle.net/21.15107/rcub_imagine_2149 .

TY  - CONF
AU  - Senćanski, Milan
AU  - Prodić, Ivana
AU  - Pantelić, Ana
AU  - Vidović, Marija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1997
AB  - Drought stress is one of the greatest threats to global food security, posing a major challenge
to agriculture. Understanding the molecular mechanisms underlying desiccation tolerance
in resurrection plants like Ramonda serbica Panc., can provide valuable insights for improving
crop resilience. Dehydrins are intrinsically disordered proteins known to accumulate in these
plants in response to desiccation. Among several proposed physiological roles, it has been
suggested that dehydrins can protect DNA from damage during water shortage. Here, we
have characterised dehydrins from R. serbica, selected a representative one and evaluated
its potential to interact with DNA.
Most of the R. serbica dehydrins were designated as hydrophilins (glycine content >6%;
GRAVY index <1). They exhibit a high disorder propensity, making them quite dynamic
in solution. Furthermore, they were predicted to localize in the nucleus. To examine the
potential interactions with DNA in silico, we have selected a representative, highly hydrophilic
dehydrin (Gravy index: –1.29) containing a high percentage of glycine (22.6%) and charged
amino acids (lysine, glutamate and aspartate). Its 3D structures were determined using the
Phyre 2 intensive modelling and AlphaFold.
The dehydrin-DNA complex was manually adjusted, following molecular dynamic simulation
(MDS) in both cases of hydration and desiccation. To simulate complete hydration, the DNAdehydrin
complex was solvated in a water box, with final dimensions of 100×69×82 Å,
neutralised with 0.15 M NaCl. The system underwent a 10,000-step energy minimization,
consecutive 1250 ps equilibration NVE (constant number of atoms, volume and energy)
heating from 10 K to 298 K and 100 ns NPT (constant number of atoms, pressure and
temperature) MD production at 1 bar, and 1 fs integration step. In all simulations, periodic
boundary conditions (PBC) were implemented and the CHARMM36 force field was used.
The obtained results revealed that selected dehydrin can interact with both minor and major
DNA grooves. The phosphate groups from the DNA molecule form salt bridges with the
positively charged lysines from polylysine, K-segment, contributing to the complex stability.
Overall, we have provided evidence for possible dehydrin-DNA interactions. However, the
exact nature and significance of these interactions is still an area of active research in vitro.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD)
EP  - 57
SP  - 57
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1997
ER  - 

@conference{
author = "Senćanski, Milan and Prodić, Ivana and Pantelić, Ana and Vidović, Marija",
year = "2023",
abstract = "Drought stress is one of the greatest threats to global food security, posing a major challenge
to agriculture. Understanding the molecular mechanisms underlying desiccation tolerance
in resurrection plants like Ramonda serbica Panc., can provide valuable insights for improving
crop resilience. Dehydrins are intrinsically disordered proteins known to accumulate in these
plants in response to desiccation. Among several proposed physiological roles, it has been
suggested that dehydrins can protect DNA from damage during water shortage. Here, we
have characterised dehydrins from R. serbica, selected a representative one and evaluated
its potential to interact with DNA.
Most of the R. serbica dehydrins were designated as hydrophilins (glycine content >6%;
GRAVY index <1). They exhibit a high disorder propensity, making them quite dynamic
in solution. Furthermore, they were predicted to localize in the nucleus. To examine the
potential interactions with DNA in silico, we have selected a representative, highly hydrophilic
dehydrin (Gravy index: –1.29) containing a high percentage of glycine (22.6%) and charged
amino acids (lysine, glutamate and aspartate). Its 3D structures were determined using the
Phyre 2 intensive modelling and AlphaFold.
The dehydrin-DNA complex was manually adjusted, following molecular dynamic simulation
(MDS) in both cases of hydration and desiccation. To simulate complete hydration, the DNAdehydrin
complex was solvated in a water box, with final dimensions of 100×69×82 Å,
neutralised with 0.15 M NaCl. The system underwent a 10,000-step energy minimization,
consecutive 1250 ps equilibration NVE (constant number of atoms, volume and energy)
heating from 10 K to 298 K and 100 ns NPT (constant number of atoms, pressure and
temperature) MD production at 1 bar, and 1 fs integration step. In all simulations, periodic
boundary conditions (PBC) were implemented and the CHARMM36 force field was used.
The obtained results revealed that selected dehydrin can interact with both minor and major
DNA grooves. The phosphate groups from the DNA molecule form salt bridges with the
positively charged lysines from polylysine, K-segment, contributing to the complex stability.
Overall, we have provided evidence for possible dehydrin-DNA interactions. However, the
exact nature and significance of these interactions is still an area of active research in vitro.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD)",
pages = "57-57",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1997"
}

Senćanski, M., Prodić, I., Pantelić, A.,& Vidović, M.. (2023). Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD). in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 57-57.
https://hdl.handle.net/21.15107/rcub_imagine_1997

Senćanski M, Prodić I, Pantelić A, Vidović M. Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD). in 4th Belgrade Bioinformatics Conference. 2023;4:57-57.
https://hdl.handle.net/21.15107/rcub_imagine_1997 .

Senćanski, Milan, Prodić, Ivana, Pantelić, Ana, Vidović, Marija, "Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD)" in 4th Belgrade Bioinformatics Conference, 4 (2023):57-57,
https://hdl.handle.net/21.15107/rcub_imagine_1997 .

TY  - CONF
AU  - Nešić, Sofija
AU  - Pantelić, Ana
AU  - Samardžić, Jelena
AU  - Nikolić, Dragana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2158
AB  - Plant growth promoting bacteria (PGPB) are microorganisms present in plant
rhizosphere that stimulate plant growth, as well as prime plants against abiotic
and biotic stress. PGPB are also located in plant intercellular space and can directly
interfere with plant pathogens. Many different mechanisms of interaction
between plants and PGPB has been shown, but the interaction through outer
membrane vesicles (OMVs) has only been proposed. OMVs are membrane-enclosed
nanostructures produced by Gram-negative bacteria. Size of OMVs can
range from 20 to 300 nm, depending on the strain. In this research, we determined
optimal method for isolation of OMVs produced by Paraburkholderia
phytofirmans PsJN, characterized the size range of OMVs, their concentration
and origin. OMVs from PGPB PsJN have been isolated us\ing two methodological
approaches: 1) differential centrifugation, ultrafiltration, and purification
using Optiprep density gradient, and 2) OMV isolation by the commercial
ExoBacteria OMV Isolation Kit that uses an affinity-based column system. Size
and concentration of isolated OMVs were analyzed using Nanoparticle Tracking
Analysis (NTA). As the OMVs are derived from outer membranes of bacteria, their
origin has been proven using an essay for detection of lipopolysaccharides. In
addition, protein profiles of OMV from PsJN were visualized by SDS-PAGE. Comparing
different methods for isolation and purification of vesicles from PsJN we
concluded that for our PGPB strain the commercial ExoBacteria OMV Isolation
Kit provides the highest yield. Quantification of OMV using NTA of OMVs produced
by P. phytofirmans PsJN, isolated with the commercial kit, showed a mean
particle numeration of 4.05*109 particles/ml. Size of OMVs isolated from PsJN
ranged from 50 to 200 nm. Detection of lipopolysaccharides confirmed that
isolated vesicles originated from outer membranes of PsJN. These findings are
the important first step in our research of the role of OMVs in PGPB interaction
with plants.
PB  - Belgrade : Faculty of Biology
C3  - ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
T1  - Characterization of outer membrane vesicles of plant growth promoting bacteria Paraburkholderia phytofirmans PsJN
EP  - 72
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2158
ER  - 

@conference{
author = "Nešić, Sofija and Pantelić, Ana and Samardžić, Jelena and Nikolić, Dragana",
year = "2023",
abstract = "Plant growth promoting bacteria (PGPB) are microorganisms present in plant
rhizosphere that stimulate plant growth, as well as prime plants against abiotic
and biotic stress. PGPB are also located in plant intercellular space and can directly
interfere with plant pathogens. Many different mechanisms of interaction
between plants and PGPB has been shown, but the interaction through outer
membrane vesicles (OMVs) has only been proposed. OMVs are membrane-enclosed
nanostructures produced by Gram-negative bacteria. Size of OMVs can
range from 20 to 300 nm, depending on the strain. In this research, we determined
optimal method for isolation of OMVs produced by Paraburkholderia
phytofirmans PsJN, characterized the size range of OMVs, their concentration
and origin. OMVs from PGPB PsJN have been isolated us\ing two methodological
approaches: 1) differential centrifugation, ultrafiltration, and purification
using Optiprep density gradient, and 2) OMV isolation by the commercial
ExoBacteria OMV Isolation Kit that uses an affinity-based column system. Size
and concentration of isolated OMVs were analyzed using Nanoparticle Tracking
Analysis (NTA). As the OMVs are derived from outer membranes of bacteria, their
origin has been proven using an essay for detection of lipopolysaccharides. In
addition, protein profiles of OMV from PsJN were visualized by SDS-PAGE. Comparing
different methods for isolation and purification of vesicles from PsJN we
concluded that for our PGPB strain the commercial ExoBacteria OMV Isolation
Kit provides the highest yield. Quantification of OMV using NTA of OMVs produced
by P. phytofirmans PsJN, isolated with the commercial kit, showed a mean
particle numeration of 4.05*109 particles/ml. Size of OMVs isolated from PsJN
ranged from 50 to 200 nm. Detection of lipopolysaccharides confirmed that
isolated vesicles originated from outer membranes of PsJN. These findings are
the important first step in our research of the role of OMVs in PGPB interaction
with plants.",
publisher = "Belgrade : Faculty of Biology",
journal = "ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture",
title = "Characterization of outer membrane vesicles of plant growth promoting bacteria Paraburkholderia phytofirmans PsJN",
pages = "72-72",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2158"
}

Nešić, S., Pantelić, A., Samardžić, J.,& Nikolić, D.. (2023). Characterization of outer membrane vesicles of plant growth promoting bacteria Paraburkholderia phytofirmans PsJN. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
Belgrade : Faculty of Biology., 72-72.
https://hdl.handle.net/21.15107/rcub_imagine_2158

Nešić S, Pantelić A, Samardžić J, Nikolić D. Characterization of outer membrane vesicles of plant growth promoting bacteria Paraburkholderia phytofirmans PsJN. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture. 2023;:72-72.
https://hdl.handle.net/21.15107/rcub_imagine_2158 .

Nešić, Sofija, Pantelić, Ana, Samardžić, Jelena, Nikolić, Dragana, "Characterization of outer membrane vesicles of plant growth promoting bacteria Paraburkholderia phytofirmans PsJN" in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture (2023):72-72,
https://hdl.handle.net/21.15107/rcub_imagine_2158 .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2023
UR  - https://thessinppo2023.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1850
AB  - Ramonda serbica Panc. is a resurrection plant that can survive long periods of desiccation and
fully restores its metabolic functions just one day after watering. The aim of this study was to
identify key candidates and metabolic pathways involved in R. serbica desiccation tolerance.
We combined differential transcriptomics and proteomics with the analysis of phenolics,
sugars, cell wall polymers and photosynthetic electron transport (PET) chain. TMT-based
proteomic analysis allowed the relative quantification of 1192 different protein groups, 408 of
which were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost
all differentially abundant proteins and transcripts related to photosynthetic processes were
downregulated in DL. Chlorophyll fluorescence measurements showed a shift from linear PET

to cyclic electron transport (CET). The levels of H2O2-scavenging enzymes, ascorbate-
glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase

(SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD
isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs;
mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation led to cell wall
remodelling related to GLP-derived H2O2/HO●

activity and pectin demethylesterification. This
comprehensive study contributes to understanding the role and regulation of important
metabolic pathways during desiccation with the final aim to help improving the drought
tolerance in crops.
PB  - International Plant Proteomics Organization (INPPO)
C3  - 5th International Conference on Plant Proteomics
T1  - Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1850
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2023",
abstract = "Ramonda serbica Panc. is a resurrection plant that can survive long periods of desiccation and
fully restores its metabolic functions just one day after watering. The aim of this study was to
identify key candidates and metabolic pathways involved in R. serbica desiccation tolerance.
We combined differential transcriptomics and proteomics with the analysis of phenolics,
sugars, cell wall polymers and photosynthetic electron transport (PET) chain. TMT-based
proteomic analysis allowed the relative quantification of 1192 different protein groups, 408 of
which were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost
all differentially abundant proteins and transcripts related to photosynthetic processes were
downregulated in DL. Chlorophyll fluorescence measurements showed a shift from linear PET

to cyclic electron transport (CET). The levels of H2O2-scavenging enzymes, ascorbate-
glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase

(SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD
isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs;
mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation led to cell wall
remodelling related to GLP-derived H2O2/HO●

activity and pectin demethylesterification. This
comprehensive study contributes to understanding the role and regulation of important
metabolic pathways during desiccation with the final aim to help improving the drought
tolerance in crops.",
publisher = "International Plant Proteomics Organization (INPPO)",
journal = "5th International Conference on Plant Proteomics",
title = "Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1850"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2023). Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.. in 5th International Conference on Plant Proteomics
International Plant Proteomics Organization (INPPO)..
https://hdl.handle.net/21.15107/rcub_imagine_1850

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.. in 5th International Conference on Plant Proteomics. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1850 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc." in 5th International Conference on Plant Proteomics (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1850 .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Milić, Dejana
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2005
AB  - The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully
recovers metabolic functions within one day upon watering. We aimed to identify key
candidates and pathways involved in desiccation tolerance in R. serbica by employing a
systems biology approach, combining transcriptomics and proteomics.
A total of 68,694 differentially expressed genes (DEGs; p-value<0.005 and abs(log2FC)≥2) were
obtained in R. serbica leaves upon desiccation. Among them, 23,935 and 26,169 genes were
upregulated and downregulated in desiccated leaves (DL) and hydrated leaves (HL), respectively.
By differential TMT-based proteomic analysis 1192 different protein groups were identified
after filtering with at least two unique peptides per protein. In total, 229 protein groups
were more abundant in HL and 179 in DL (p-value<0.05 and abs(FC)≥1.3). The majority
of the DAPs and DEGs involved in photosynthesis, transport, secondary metabolism, and
signaling, were less abundant in DL. On the other hand, proteins and transcripts associated
with fermentation, N-metabolism, heme, protein synthesis, folding and assembly, C1-
metabolism, and late embryogenesis abundant proteins, were more accumulated in DL.
A poor correlation between proteomic and transcriptomic results was detected for mitochondrial
electron transport and ATP production, gluconeogenesis, glycolysis, tricarboxylic
acid cycle, and enzymatic H2O2 scavengers due to different mRNA half-life, protein turnover,
dynamic posttranscriptional and posttranslational modifications. Finally, desiccation tolerance
in R. serbica is a species-specific process orchestrated by several metabolic pathways
that are temporally and compartmentally regulated at several levels.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.
EP  - 65
SP  - 65
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2005
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Milić, Dejana and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2023",
abstract = "The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully
recovers metabolic functions within one day upon watering. We aimed to identify key
candidates and pathways involved in desiccation tolerance in R. serbica by employing a
systems biology approach, combining transcriptomics and proteomics.
A total of 68,694 differentially expressed genes (DEGs; p-value<0.005 and abs(log2FC)≥2) were
obtained in R. serbica leaves upon desiccation. Among them, 23,935 and 26,169 genes were
upregulated and downregulated in desiccated leaves (DL) and hydrated leaves (HL), respectively.
By differential TMT-based proteomic analysis 1192 different protein groups were identified
after filtering with at least two unique peptides per protein. In total, 229 protein groups
were more abundant in HL and 179 in DL (p-value<0.05 and abs(FC)≥1.3). The majority
of the DAPs and DEGs involved in photosynthesis, transport, secondary metabolism, and
signaling, were less abundant in DL. On the other hand, proteins and transcripts associated
with fermentation, N-metabolism, heme, protein synthesis, folding and assembly, C1-
metabolism, and late embryogenesis abundant proteins, were more accumulated in DL.
A poor correlation between proteomic and transcriptomic results was detected for mitochondrial
electron transport and ATP production, gluconeogenesis, glycolysis, tricarboxylic
acid cycle, and enzymatic H2O2 scavengers due to different mRNA half-life, protein turnover,
dynamic posttranscriptional and posttranslational modifications. Finally, desiccation tolerance
in R. serbica is a species-specific process orchestrated by several metabolic pathways
that are temporally and compartmentally regulated at several levels.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.",
pages = "65-65",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2005"
}

Vidović, M., Battisti, I., Pantelić, A., Milić, D., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2023). Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 65-65.
https://hdl.handle.net/21.15107/rcub_imagine_2005

Vidović M, Battisti I, Pantelić A, Milić D, Arrigoni G, Masi A, Veljović Jovanović S. Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference. 2023;4:65-65.
https://hdl.handle.net/21.15107/rcub_imagine_2005 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Milić, Dejana, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc." in 4th Belgrade Bioinformatics Conference, 4 (2023):65-65,
https://hdl.handle.net/21.15107/rcub_imagine_2005 .

TY  - CONF
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Milić Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2008
AB  - Ramonda serbica Panc. is an ancient resurrection plant, that survives a long desiccation period
and fully recovers metabolic functions upon watering. The main characteristic of desiccationtolerant
plant species is their ability to accumulate protective late embryogenesis abounded
protein (LEAPs). To propose their role in R. serbica desiccation tolerance we structurally
analysed LEAPs in hydrated and desiccated leaves.
According to transcriptomics, 318 LEAPs were identified and classified into seven family
groups based on protein BLAST analysis and conserved motifs (Pfam). The largest LEAPs
belonged to the LEA2 and LEA4 protein family groups. We employed online tools to analyse
physicochemical characteristics (Expasy, ProtParam, BioPython, GRAVY calculator), disorder
propensity, and characterization protein structures (FELLS, JPred, SOPMA, PsiPred, Phyre2,
Espritz-DisProt, Espritz-X, Iupred, TMHMM, +Heliquest).
The most abundant, atypical LEA2 group containing 127, mostly hydrophobic proteins, was
divided into five subgroups. Members of this group were predicted to fold into globular
domains, β-barrel at the C-terminus, followed by transmembrane hydrophobic-helices and
disordered N-terminal regions. Results indicated the possible involvement in the protection
of the chloroplastic membranes.
The LEA4 group exhibited an exceptionally high tendency to form amphipathic α-helices and
simultaneously had a high disorder propensity. This group is made of 96 proteins, classified into 3
subgroups. The high content of polar and charged amino acids (lysine, glutamate, and aspartate) is
characteristic of this group. Motifs corresponding to the R. serbica LEA4 protein family group folded
into A-type α-helices that contained positive, negative, and hydrophobic surfaces. Based on previous
knowledge, the possible functions of the LEA2 and LEA4 groups are discussed with significant
implications on cell preservation technology and the improvement of crop drought tolerance.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.
EP  - 68
SP  - 68
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2008
ER  - 

@conference{
author = "Pantelić, Ana and Stevanović, Strahinja and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2023",
abstract = "Ramonda serbica Panc. is an ancient resurrection plant, that survives a long desiccation period
and fully recovers metabolic functions upon watering. The main characteristic of desiccationtolerant
plant species is their ability to accumulate protective late embryogenesis abounded
protein (LEAPs). To propose their role in R. serbica desiccation tolerance we structurally
analysed LEAPs in hydrated and desiccated leaves.
According to transcriptomics, 318 LEAPs were identified and classified into seven family
groups based on protein BLAST analysis and conserved motifs (Pfam). The largest LEAPs
belonged to the LEA2 and LEA4 protein family groups. We employed online tools to analyse
physicochemical characteristics (Expasy, ProtParam, BioPython, GRAVY calculator), disorder
propensity, and characterization protein structures (FELLS, JPred, SOPMA, PsiPred, Phyre2,
Espritz-DisProt, Espritz-X, Iupred, TMHMM, +Heliquest).
The most abundant, atypical LEA2 group containing 127, mostly hydrophobic proteins, was
divided into five subgroups. Members of this group were predicted to fold into globular
domains, β-barrel at the C-terminus, followed by transmembrane hydrophobic-helices and
disordered N-terminal regions. Results indicated the possible involvement in the protection
of the chloroplastic membranes.
The LEA4 group exhibited an exceptionally high tendency to form amphipathic α-helices and
simultaneously had a high disorder propensity. This group is made of 96 proteins, classified into 3
subgroups. The high content of polar and charged amino acids (lysine, glutamate, and aspartate) is
characteristic of this group. Motifs corresponding to the R. serbica LEA4 protein family group folded
into A-type α-helices that contained positive, negative, and hydrophobic surfaces. Based on previous
knowledge, the possible functions of the LEA2 and LEA4 groups are discussed with significant
implications on cell preservation technology and the improvement of crop drought tolerance.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.",
pages = "68-68",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2008"
}

Pantelić, A., Stevanović, S., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2023). Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 68-68.
https://hdl.handle.net/21.15107/rcub_imagine_2008

Pantelić A, Stevanović S, Milić Komić S, Kilibarda N, Vidović M. Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference. 2023;4:68-68.
https://hdl.handle.net/21.15107/rcub_imagine_2008 .

Pantelić, Ana, Stevanović, Strahinja, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc." in 4th Belgrade Bioinformatics Conference, 4 (2023):68-68,
https://hdl.handle.net/21.15107/rcub_imagine_2008 .

TY  - CONF
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Samardžić, Jelena
AU  - Banović Đeri, Bojana
AU  - Vidović, Marija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2035
AB  - Variegated Pelargonium zonale is a widely cultivated ornamental plant characterized by
green, photosynthetically active tissue (GL) and white, non-photosynthetic tissue (WL).
The aim of this study was to investigate the transcriptomic differences between these
two tissue types.
We performed RNA-seq analysis of GL and WL on Illumina HiSeq 2500 platform. The
raw reads were processed using in-house scripts to remove low-quality reads, adapter
sequences, poly-N sequences, and contaminants. High-quality clean reads were subjected
to de novo transcriptome assembly using Trinity (min_kmer_cov = 2, min_glue = 2). The
redundancy was removed and longest transcripts per cluster were selected as unigenes.
Gene expression levels were estimated using RSEM by mapping clean data back to the
assembled transcriptome (Bowtie2 with mismatch = 0). Differential expression analysis
between GL and WL (three biological replicates per each) was performed with DESeq2
R package (p values adjusted according to Benjamini and Hochberg for controlling False
Discovery Rate). Genes with abs (log2 FC) ≥ 2 and adjusted p value < 0.05 were assigned
as statistically significant differentially expressed. Functional enrichment analysis was
performed using GOseq R package and KOBAS software (corrected p < 0.05).
We annotated 85,374 unigenes (61.17%), providing a valuable resource for future
functional genomics studies. Out of 8896 gene clusters that were statistically significantly
differentially expressed between the green and white leaf tissues (p value < 0.05 and
abs(log2 fold change) ≥ 2), 5585 were upregulated in the WL, while 3311 were upregulated
in the GL. These findings shed light on the transcriptomic differences between the
two leaf tissue types in P. zonale and provide a foundation for further research on the
functional significance of these differences. Also, this study demonstrated utility of the
Trinity pipeline for de novo transcriptomic analysis of organism whose genomes are yet
not sequenced.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale
EP  - 90
SP  - 90
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2035
ER  - 

@conference{
author = "Milić, Dejana and Pantelić, Ana and Samardžić, Jelena and Banović Đeri, Bojana and Vidović, Marija",
year = "2023",
abstract = "Variegated Pelargonium zonale is a widely cultivated ornamental plant characterized by
green, photosynthetically active tissue (GL) and white, non-photosynthetic tissue (WL).
The aim of this study was to investigate the transcriptomic differences between these
two tissue types.
We performed RNA-seq analysis of GL and WL on Illumina HiSeq 2500 platform. The
raw reads were processed using in-house scripts to remove low-quality reads, adapter
sequences, poly-N sequences, and contaminants. High-quality clean reads were subjected
to de novo transcriptome assembly using Trinity (min_kmer_cov = 2, min_glue = 2). The
redundancy was removed and longest transcripts per cluster were selected as unigenes.
Gene expression levels were estimated using RSEM by mapping clean data back to the
assembled transcriptome (Bowtie2 with mismatch = 0). Differential expression analysis
between GL and WL (three biological replicates per each) was performed with DESeq2
R package (p values adjusted according to Benjamini and Hochberg for controlling False
Discovery Rate). Genes with abs (log2 FC) ≥ 2 and adjusted p value < 0.05 were assigned
as statistically significant differentially expressed. Functional enrichment analysis was
performed using GOseq R package and KOBAS software (corrected p < 0.05).
We annotated 85,374 unigenes (61.17%), providing a valuable resource for future
functional genomics studies. Out of 8896 gene clusters that were statistically significantly
differentially expressed between the green and white leaf tissues (p value < 0.05 and
abs(log2 fold change) ≥ 2), 5585 were upregulated in the WL, while 3311 were upregulated
in the GL. These findings shed light on the transcriptomic differences between the
two leaf tissue types in P. zonale and provide a foundation for further research on the
functional significance of these differences. Also, this study demonstrated utility of the
Trinity pipeline for de novo transcriptomic analysis of organism whose genomes are yet
not sequenced.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale",
pages = "90-90",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2035"
}

Milić, D., Pantelić, A., Samardžić, J., Banović Đeri, B.,& Vidović, M.. (2023). Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 90-90.
https://hdl.handle.net/21.15107/rcub_imagine_2035

Milić D, Pantelić A, Samardžić J, Banović Đeri B, Vidović M. Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale. in 4th Belgrade Bioinformatics Conference. 2023;4:90-90.
https://hdl.handle.net/21.15107/rcub_imagine_2035 .

Milić, Dejana, Pantelić, Ana, Samardžić, Jelena, Banović Đeri, Bojana, Vidović, Marija, "Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale" in 4th Belgrade Bioinformatics Conference, 4 (2023):90-90,
https://hdl.handle.net/21.15107/rcub_imagine_2035 .

TY  - CONF
AU  - Pantelić, Ana
AU  - Senćanski, Milan
AU  - Prodić, Ivana
AU  - Milić, Dejana
AU  - Vidović, Marija
PY  - 2023
UR  - https://ml4ngp.eu/conference-bratislava/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1934
PB  - European Cooperation in Science and Technology
C3  - Non-globular proteins in the era of machine learning
T1  - Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation
EP  - 6
SP  - 5
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1934
ER  - 

@conference{
author = "Pantelić, Ana and Senćanski, Milan and Prodić, Ivana and Milić, Dejana and Vidović, Marija",
year = "2023",
publisher = "European Cooperation in Science and Technology",
journal = "Non-globular proteins in the era of machine learning",
title = "Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation",
pages = "6-5",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1934"
}

Pantelić, A., Senćanski, M., Prodić, I., Milić, D.,& Vidović, M.. (2023). Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation. in Non-globular proteins in the era of machine learning
European Cooperation in Science and Technology., 5-6.
https://hdl.handle.net/21.15107/rcub_imagine_1934

Pantelić A, Senćanski M, Prodić I, Milić D, Vidović M. Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation. in Non-globular proteins in the era of machine learning. 2023;:5-6.
https://hdl.handle.net/21.15107/rcub_imagine_1934 .

Pantelić, Ana, Senćanski, Milan, Prodić, Ivana, Milić, Dejana, Vidović, Marija, "Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation" in Non-globular proteins in the era of machine learning (2023):5-6,
https://hdl.handle.net/21.15107/rcub_imagine_1934 .

TY  - CONF
AU  - Vidović, Marija
AU  - Pantelić, Ana
AU  - Senćanski, Milan
AU  - Prodić, Ivana
PY  - 2023
UR  - https://ml4ngp.eu/conference-bratislava/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1933
PB  - European Cooperation in Science and Technology
C3  - Non-globular proteins in the era of machine learning
T1  - LEA4 proteins: How disordered are they?
EP  - 50
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1933
ER  - 

@conference{
author = "Vidović, Marija and Pantelić, Ana and Senćanski, Milan and Prodić, Ivana",
year = "2023",
publisher = "European Cooperation in Science and Technology",
journal = "Non-globular proteins in the era of machine learning",
title = "LEA4 proteins: How disordered are they?",
pages = "50-49",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1933"
}

Vidović, M., Pantelić, A., Senćanski, M.,& Prodić, I.. (2023). LEA4 proteins: How disordered are they?. in Non-globular proteins in the era of machine learning
European Cooperation in Science and Technology., 49-50.
https://hdl.handle.net/21.15107/rcub_imagine_1933

Vidović M, Pantelić A, Senćanski M, Prodić I. LEA4 proteins: How disordered are they?. in Non-globular proteins in the era of machine learning. 2023;:49-50.
https://hdl.handle.net/21.15107/rcub_imagine_1933 .

Vidović, Marija, Pantelić, Ana, Senćanski, Milan, Prodić, Ivana, "LEA4 proteins: How disordered are they?" in Non-globular proteins in the era of machine learning (2023):49-50,
https://hdl.handle.net/21.15107/rcub_imagine_1933 .

TY  - JOUR
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Banović Đeri, Bojana
AU  - Samardžić, Jelena
AU  - Vidović, Marija
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/6/5288
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1795
AB  - The photosynthetically active green leaf (GL) and non-active white leaf (WL) tissues of variegated Pelargonium zonale provide an excellent model system for studying processes associated with photosynthesis and sink-source interactions, enabling the same microenvironmental conditions. By combining differential transcriptomics and metabolomics, we identified the main differences between these two metabolically contrasting tissues. Genes related to photosynthesis and associated pigments, the Calvin–Benson cycle, fermentation, and glycolysis were strongly repressed in WL. On the other hand, genes related to nitrogen and protein metabolism, defence, cytoskeletal components (motor proteins), cell division, DNA replication, repair and recombination, chromatin remodelling, and histone modifications were upregulated in WL. A content of soluble sugars, TCA intermediates, ascorbate, and hydroxybenzoic acids was lower, while the concentration of free amino acids (AAs), hydroxycinnamic acids, and several quercetin and kaempferol glycosides was higher in WL than in GL. Therefore, WL presents a carbon sink and depends on photosynthetic and energy-generating processes in GL. Furthermore, the upregulated nitrogen metabolism in WL compensates for the insufficient energy from carbon metabolism by providing alternative respiratory substrates. At the same time, WL serves as nitrogen storage. Overall, our study provides a new genetic data resource for the use of this excellent model system and for ornamental pelargonium breeding and contributes to uncovering molecular mechanisms underlying variegation and its adaptive ecological value.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study
IS  - 6
SP  - 5288
VL  - 24
DO  - 10.3390/ijms24065288
ER  - 

@article{
author = "Milić, Dejana and Pantelić, Ana and Banović Đeri, Bojana and Samardžić, Jelena and Vidović, Marija",
year = "2023",
abstract = "The photosynthetically active green leaf (GL) and non-active white leaf (WL) tissues of variegated Pelargonium zonale provide an excellent model system for studying processes associated with photosynthesis and sink-source interactions, enabling the same microenvironmental conditions. By combining differential transcriptomics and metabolomics, we identified the main differences between these two metabolically contrasting tissues. Genes related to photosynthesis and associated pigments, the Calvin–Benson cycle, fermentation, and glycolysis were strongly repressed in WL. On the other hand, genes related to nitrogen and protein metabolism, defence, cytoskeletal components (motor proteins), cell division, DNA replication, repair and recombination, chromatin remodelling, and histone modifications were upregulated in WL. A content of soluble sugars, TCA intermediates, ascorbate, and hydroxybenzoic acids was lower, while the concentration of free amino acids (AAs), hydroxycinnamic acids, and several quercetin and kaempferol glycosides was higher in WL than in GL. Therefore, WL presents a carbon sink and depends on photosynthetic and energy-generating processes in GL. Furthermore, the upregulated nitrogen metabolism in WL compensates for the insufficient energy from carbon metabolism by providing alternative respiratory substrates. At the same time, WL serves as nitrogen storage. Overall, our study provides a new genetic data resource for the use of this excellent model system and for ornamental pelargonium breeding and contributes to uncovering molecular mechanisms underlying variegation and its adaptive ecological value.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study",
number = "6",
pages = "5288",
volume = "24",
doi = "10.3390/ijms24065288"
}

Milić, D., Pantelić, A., Banović Đeri, B., Samardžić, J.,& Vidović, M.. (2023). Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study. in International Journal of Molecular Sciences, 24(6), 5288.
https://doi.org/10.3390/ijms24065288

Milić D, Pantelić A, Banović Đeri B, Samardžić J, Vidović M. Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study. in International Journal of Molecular Sciences. 2023;24(6):5288.
doi:10.3390/ijms24065288 .

Milić, Dejana, Pantelić, Ana, Banović Đeri, Bojana, Samardžić, Jelena, Vidović, Marija, "Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study" in International Journal of Molecular Sciences, 24, no. 6 (2023):5288,
https://doi.org/10.3390/ijms24065288 . .

TY  - CONF
AU  - Banović Đeri, Bojana
AU  - Nešić, Sofija
AU  - Pantelić, Ana
AU  - Samardžić, Jelena
AU  - Nikolić, Dragana
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2030
AB  - Bacterial small RNAs (sRNAs) represent a highly diverse RNA class ranging from 8 to 200
nucleotides in length, originating from the bacterial chromosome, plasmids or phages.
After syntheses sRNAs can remain inside the bacterial cell, be secreted or packed into
outer membrane vesicles (OMV), enabling various intra- and inter-kingdom interactions.
Different sRNAs biotypes display differences in structure, mechanism of action and level
of regulation (i.e. transcription, translation, mRNA stability, etc.), but could be broadly
grouped in: trans-acting sRNAs (bind to target mRNAs) and cis-encoded sRNAs (or
antisense RNA that may interact not only with mRNAs, but also with proteins and DNA).
Even though the advancement of high-throughput sequencing technology led to a burst
of knowledge on small RNAs complexity and diversity, there are still specific challenges
related to sRNA-seq data analysis that need to be resolved. Two main challenges,
associated to short length of many bacterial sRNA biotypes, are: (i) to discriminate
between functional sRNAs synthesized by bacterial cell and degradation fragments
produced by sample preparation and (ii) to detect functional sRNAs displaying sequence
variation. While loss of very small sized sRNAs could easily be overcome by cutting-off
only the specific adapter sequences that were used in sRNA library preparation, providing
a proper mapping still remains a strenuous task.
The aim of this study was to test five different mapping tools that are widely used in NGS
data analysis (bbmap, bowtie2, bwa, minimap2 and segemehl) for their performances in
mapping of bacterial OMV sRNA-seq data to bacterial reference genome. For this test
publicly available NCBI sRNA-seq dataset from OMVs of Aliivibrio fischeri (PRJNA629425)
was used, as it contained sRNAs of different length and biotype and because A.fischeri
reference genome and annotation were available (PRJNA12986). We evaluated five
mappers using alignment and assignment rates as well as computational time. Alignment
rate was calculated as the ratio of aligned and input reads, while the assignment rate
was calculated as the ratio of assigned and aligned reads. Finally, totals of detected
sRNAs biotypes were compared between different mappers. The statistical analysis was
performed in R (version 4.3.0) and performance metrics are discussed.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Impact of different mapping tools on detection of small RNAs in bacterial outer membrane vesicles
EP  - 85
SP  - 85
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2030
ER  - 

@conference{
author = "Banović Đeri, Bojana and Nešić, Sofija and Pantelić, Ana and Samardžić, Jelena and Nikolić, Dragana",
year = "2023",
abstract = "Bacterial small RNAs (sRNAs) represent a highly diverse RNA class ranging from 8 to 200
nucleotides in length, originating from the bacterial chromosome, plasmids or phages.
After syntheses sRNAs can remain inside the bacterial cell, be secreted or packed into
outer membrane vesicles (OMV), enabling various intra- and inter-kingdom interactions.
Different sRNAs biotypes display differences in structure, mechanism of action and level
of regulation (i.e. transcription, translation, mRNA stability, etc.), but could be broadly
grouped in: trans-acting sRNAs (bind to target mRNAs) and cis-encoded sRNAs (or
antisense RNA that may interact not only with mRNAs, but also with proteins and DNA).
Even though the advancement of high-throughput sequencing technology led to a burst
of knowledge on small RNAs complexity and diversity, there are still specific challenges
related to sRNA-seq data analysis that need to be resolved. Two main challenges,
associated to short length of many bacterial sRNA biotypes, are: (i) to discriminate
between functional sRNAs synthesized by bacterial cell and degradation fragments
produced by sample preparation and (ii) to detect functional sRNAs displaying sequence
variation. While loss of very small sized sRNAs could easily be overcome by cutting-off
only the specific adapter sequences that were used in sRNA library preparation, providing
a proper mapping still remains a strenuous task.
The aim of this study was to test five different mapping tools that are widely used in NGS
data analysis (bbmap, bowtie2, bwa, minimap2 and segemehl) for their performances in
mapping of bacterial OMV sRNA-seq data to bacterial reference genome. For this test
publicly available NCBI sRNA-seq dataset from OMVs of Aliivibrio fischeri (PRJNA629425)
was used, as it contained sRNAs of different length and biotype and because A.fischeri
reference genome and annotation were available (PRJNA12986). We evaluated five
mappers using alignment and assignment rates as well as computational time. Alignment
rate was calculated as the ratio of aligned and input reads, while the assignment rate
was calculated as the ratio of assigned and aligned reads. Finally, totals of detected
sRNAs biotypes were compared between different mappers. The statistical analysis was
performed in R (version 4.3.0) and performance metrics are discussed.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Impact of different mapping tools on detection of small RNAs in bacterial outer membrane vesicles",
pages = "85-85",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2030"
}

Banović Đeri, B., Nešić, S., Pantelić, A., Samardžić, J.,& Nikolić, D.. (2023). Impact of different mapping tools on detection of small RNAs in bacterial outer membrane vesicles. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 85-85.
https://hdl.handle.net/21.15107/rcub_imagine_2030

Banović Đeri B, Nešić S, Pantelić A, Samardžić J, Nikolić D. Impact of different mapping tools on detection of small RNAs in bacterial outer membrane vesicles. in 4th Belgrade Bioinformatics Conference. 2023;4:85-85.
https://hdl.handle.net/21.15107/rcub_imagine_2030 .

Banović Đeri, Bojana, Nešić, Sofija, Pantelić, Ana, Samardžić, Jelena, Nikolić, Dragana, "Impact of different mapping tools on detection of small RNAs in bacterial outer membrane vesicles" in 4th Belgrade Bioinformatics Conference, 4 (2023):85-85,
https://hdl.handle.net/21.15107/rcub_imagine_2030 .

TY  - CONF
AU  - Golob, Aleksandra
AU  - Ojdanič, Nik
AU  - Živanović, Bojana
AU  - Germ, Mateja
AU  - Milić Komić, Sonja
AU  - Sedlarević Zorić, Ana
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Mavrič Čermelj, Anja
AU  - Samardžić, Jelena
AU  - Veljovic Jovanovic, Sonja
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1839
AB  - Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered abeneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperaturesby promoting antioxidant production. However, the underlying mechanisms are still notelucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed towater shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impactsphotochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflectiveand transmissive properties. Si application between flag leaf emergence and grain fillinghad the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was themost abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was theonly hydroxycinnamic acid showing significant accumulation with the latest applied Si comparedto no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to droughtwithout Si addition, while lysine was the most accumulated in the leaves supplemented by Si atthe grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to droughtregardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, itseffect was dependent on the barley growth phase prior to supplementation.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting
T1  - The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought
SP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1839
ER  - 

@conference{
author = "Golob, Aleksandra and Ojdanič, Nik and Živanović, Bojana and Germ, Mateja and Milić Komić, Sonja and Sedlarević Zorić, Ana and Milić, Dejana and Pantelić, Ana and Mavrič Čermelj, Anja and Samardžić, Jelena and Veljovic Jovanovic, Sonja and Vidović, Marija",
year = "2022",
abstract = "Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered abeneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperaturesby promoting antioxidant production. However, the underlying mechanisms are still notelucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed towater shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impactsphotochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflectiveand transmissive properties. Si application between flag leaf emergence and grain fillinghad the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was themost abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was theonly hydroxycinnamic acid showing significant accumulation with the latest applied Si comparedto no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to droughtwithout Si addition, while lysine was the most accumulated in the leaves supplemented by Si atthe grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to droughtregardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, itseffect was dependent on the barley growth phase prior to supplementation.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting",
title = "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought",
pages = "67",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1839"
}

Golob, A., Ojdanič, N., Živanović, B., Germ, M., Milić Komić, S., Sedlarević Zorić, A., Milić, D., Pantelić, A., Mavrič Čermelj, A., Samardžić, J., Veljovic Jovanovic, S.,& Vidović, M.. (2022). The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in 4th International Conference on Plant Biology and 23rd SPPS Meeting
Serbian Plant Physiology Society., 67.
https://hdl.handle.net/21.15107/rcub_imagine_1839

Golob A, Ojdanič N, Živanović B, Germ M, Milić Komić S, Sedlarević Zorić A, Milić D, Pantelić A, Mavrič Čermelj A, Samardžić J, Veljovic Jovanovic S, Vidović M. The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:67.
https://hdl.handle.net/21.15107/rcub_imagine_1839 .

Golob, Aleksandra, Ojdanič, Nik, Živanović, Bojana, Germ, Mateja, Milić Komić, Sonja, Sedlarević Zorić, Ana, Milić, Dejana, Pantelić, Ana, Mavrič Čermelj, Anja, Samardžić, Jelena, Veljovic Jovanovic, Sonja, Vidović, Marija, "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):67,
https://hdl.handle.net/21.15107/rcub_imagine_1839 .

TY  - CONF
AU  - Vidović, Marija
AU  - Tosti, T.
AU  - Nikolić, N.
AU  - Pantelić, Ana
AU  - Veljović Jovanović, S.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1868
UR  - http://www.sfses.com/docs/14th-SFSES-Abstracts.pdf
AB  - Resurrection plant Ramonda serbica Panc. survives long desiccation periods
and fully recovers metabolic functions already one day upon watering. This study
aimed to investigate the role of soluble sugars and polyphenols, as well as cell wallassociated
polysaccharides and lignin in desiccation tolerance in R. serbica, an
endemic species from the Balkan peninsula. We combined differential
transcriptomics and proteomics, the analysis of soluble polyphenolics and sugars, as
well as FTIR analysis of the cell wall polymers. Pectin, cellulose, hemicellulose, and
xyloglucans were identified as polysaccharide components of the R. serbica cell
wall and they decreased upon desiccation. Desiccation provoked cell wall
remodelling related to the possible production of H2O2/HO via germin-like proteins
and pectin demethylesterification. In addition, desiccation induced carbon recycling
from starch to soluble sugar osmolytes, whose content significantly increased in
desiccated leaves. These data support the importance of specific sugars and the
plasticity of the cell wall as one of the major contributors to desiccation tolerance of
resurrection species, contributing to further crop drought tolerance improvement.
PB  - Belgrade : Institute for Nature Conservation of Serbia
PB  - Niš : Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions
T1  - The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1868
ER  - 

@conference{
author = "Vidović, Marija and Tosti, T. and Nikolić, N. and Pantelić, Ana and Veljović Jovanović, S.",
year = "2022",
abstract = "Resurrection plant Ramonda serbica Panc. survives long desiccation periods
and fully recovers metabolic functions already one day upon watering. This study
aimed to investigate the role of soluble sugars and polyphenols, as well as cell wallassociated
polysaccharides and lignin in desiccation tolerance in R. serbica, an
endemic species from the Balkan peninsula. We combined differential
transcriptomics and proteomics, the analysis of soluble polyphenolics and sugars, as
well as FTIR analysis of the cell wall polymers. Pectin, cellulose, hemicellulose, and
xyloglucans were identified as polysaccharide components of the R. serbica cell
wall and they decreased upon desiccation. Desiccation provoked cell wall
remodelling related to the possible production of H2O2/HO via germin-like proteins
and pectin demethylesterification. In addition, desiccation induced carbon recycling
from starch to soluble sugar osmolytes, whose content significantly increased in
desiccated leaves. These data support the importance of specific sugars and the
plasticity of the cell wall as one of the major contributors to desiccation tolerance of
resurrection species, contributing to further crop drought tolerance improvement.",
publisher = "Belgrade : Institute for Nature Conservation of Serbia, Niš : Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions",
title = "The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1868"
}

Vidović, M., Tosti, T., Nikolić, N., Pantelić, A.,& Veljović Jovanović, S.. (2022). The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions
Belgrade : Institute for Nature Conservation of Serbia., 73.
https://hdl.handle.net/21.15107/rcub_imagine_1868

Vidović M, Tosti T, Nikolić N, Pantelić A, Veljović Jovanović S. The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions. 2022;:73.
https://hdl.handle.net/21.15107/rcub_imagine_1868 .

Vidović, Marija, Tosti, T., Nikolić, N., Pantelić, Ana, Veljović Jovanović, S., "The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions (2022):73,
https://hdl.handle.net/21.15107/rcub_imagine_1868 .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Veljović Jovanović, Sonja
AU  - Pantelić, Ana
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1867
AB  - Ressurection plants are extraordinary because of their ability to withstand long periods
without water, enter a state of anhydrobiosis, and fully recover upon water arrival.
Ramonda serbica is a relic and endemic species that belong to a very small group of
desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental
conditions have been an appealing subject to many researchers. Most of the genes
responsible for this amazing ability are present in other plants, and this path of research
where those genes could be activated in crops is growing much more attention because of
the imminent crisis regarding food supplies in the near future. Key components involved in
the response to dehydration in R. serbica plants were analysed through a comprehensive
transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis
abundant proteins play a significant role in the complex defence processes involved in
desiccation tolerance. Defining physicochemical characteristics and specific physiological
functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability
in other areas of research.
PB  - Belgrade : Faculty of Chemistry, Serbian Biochemical Societ
C3  - Amazing Biochemistry
T1  - Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein
SP  - 37
VL  - 11
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1867
ER  - 

@conference{
author = "Milić Komić, Sonja and Veljović Jovanović, Sonja and Pantelić, Ana and Vidović, Marija",
year = "2022",
abstract = "Ressurection plants are extraordinary because of their ability to withstand long periods
without water, enter a state of anhydrobiosis, and fully recover upon water arrival.
Ramonda serbica is a relic and endemic species that belong to a very small group of
desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental
conditions have been an appealing subject to many researchers. Most of the genes
responsible for this amazing ability are present in other plants, and this path of research
where those genes could be activated in crops is growing much more attention because of
the imminent crisis regarding food supplies in the near future. Key components involved in
the response to dehydration in R. serbica plants were analysed through a comprehensive
transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis
abundant proteins play a significant role in the complex defence processes involved in
desiccation tolerance. Defining physicochemical characteristics and specific physiological
functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability
in other areas of research.",
publisher = "Belgrade : Faculty of Chemistry, Serbian Biochemical Societ",
journal = "Amazing Biochemistry",
title = "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein",
pages = "37",
volume = "11",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1867"
}

Milić Komić, S., Veljović Jovanović, S., Pantelić, A.,& Vidović, M.. (2022). Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Amazing Biochemistry
Belgrade : Faculty of Chemistry, Serbian Biochemical Societ., 11, 37.
https://hdl.handle.net/21.15107/rcub_imagine_1867

Milić Komić S, Veljović Jovanović S, Pantelić A, Vidović M. Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Amazing Biochemistry. 2022;11:37.
https://hdl.handle.net/21.15107/rcub_imagine_1867 .

Milić Komić, Sonja, Veljović Jovanović, Sonja, Pantelić, Ana, Vidović, Marija, "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein" in Amazing Biochemistry, 11 (2022):37,
https://hdl.handle.net/21.15107/rcub_imagine_1867 .

TY  - CONF
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Banović Đeri, Bojana
AU  - Samardžić, Jelena
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1848
AB  - In the global warming era, we are facing extreme changes in environmental conditions such
as high and low temperatures, high light and ultraviolet radiation intensity, as well as drought
and increased CO2 levels. The effects of these factors on plants are often interrelated and usually
result in a disturbed balance between the amount of energy received and the ability to process
it. In order to balance energy input and prevent photooxidative damage, plants have evolved
multiple mechanisms for energy dissipation and photoprotection. To efficiently dissipate the excess
excitation energy (EEE), the additional electron sinks, such as photorespiration, biosynthesis
of phenolics and nitrate reduction are stimulated. Variegated leaves of Pelargonium zonale have
been proven to be a suitable model system for examining ‘source–sink’ interactions within the
same leaf, providing the same microenvironment conditions, unlike common root-shoot studies.
The aim of our study was to investigate carbon and nitrogen reallocation between photosynthetically
active (green leaf sectors) and photosynthetically inactive (white leaf sectors) under EEE
pressure induced by low temperature in combination with high light intensity. Besides the amino
acids and phenolics analyses, we monitored morphological and photosynthetic parameters of
P. zonale leaves. Our results revealed higher content of free proteogenic amino acids in the white
leaf sectors than in the green ones. Moreover, EEE triggered increased polyphenol synthesis, namely
anthocyanins, already on the fourth day of the experiment. Also, the leaf fresh/dry weight (FW/
DW) ratio was significantly lower in plants exposed to EEE, indicating possible cell wall stiffening.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022
T1  - The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1848
ER  - 

@conference{
author = "Milić, Dejana and Pantelić, Ana and Banović Đeri, Bojana and Samardžić, Jelena and Vidović, Marija",
year = "2022",
abstract = "In the global warming era, we are facing extreme changes in environmental conditions such
as high and low temperatures, high light and ultraviolet radiation intensity, as well as drought
and increased CO2 levels. The effects of these factors on plants are often interrelated and usually
result in a disturbed balance between the amount of energy received and the ability to process
it. In order to balance energy input and prevent photooxidative damage, plants have evolved
multiple mechanisms for energy dissipation and photoprotection. To efficiently dissipate the excess
excitation energy (EEE), the additional electron sinks, such as photorespiration, biosynthesis
of phenolics and nitrate reduction are stimulated. Variegated leaves of Pelargonium zonale have
been proven to be a suitable model system for examining ‘source–sink’ interactions within the
same leaf, providing the same microenvironment conditions, unlike common root-shoot studies.
The aim of our study was to investigate carbon and nitrogen reallocation between photosynthetically
active (green leaf sectors) and photosynthetically inactive (white leaf sectors) under EEE
pressure induced by low temperature in combination with high light intensity. Besides the amino
acids and phenolics analyses, we monitored morphological and photosynthetic parameters of
P. zonale leaves. Our results revealed higher content of free proteogenic amino acids in the white
leaf sectors than in the green ones. Moreover, EEE triggered increased polyphenol synthesis, namely
anthocyanins, already on the fourth day of the experiment. Also, the leaf fresh/dry weight (FW/
DW) ratio was significantly lower in plants exposed to EEE, indicating possible cell wall stiffening.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022",
title = "The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study",
pages = "66",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1848"
}

Milić, D., Pantelić, A., Banović Đeri, B., Samardžić, J.,& Vidović, M.. (2022). The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 66.
https://hdl.handle.net/21.15107/rcub_imagine_1848

Milić D, Pantelić A, Banović Đeri B, Samardžić J, Vidović M. The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022. 2022;:66.
https://hdl.handle.net/21.15107/rcub_imagine_1848 .

Milić, Dejana, Pantelić, Ana, Banović Đeri, Bojana, Samardžić, Jelena, Vidović, Marija, "The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study" in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022 (2022):66,
https://hdl.handle.net/21.15107/rcub_imagine_1848 .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1847
AB  - Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study was to identify key candidates and pathways involved in desiccation
tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,
proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the
cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain
were performed. In total, 1192 different protein groups were quantified by TMT-based comparative
quantitative proteomics. Among them, 408 protein groups showed a statistically significant
difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the
majority of proteins related to photosynthetic processes were less abundant, while chlorophyll
fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic
electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione
cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were
reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins
(GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),
were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall
polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/
HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific
process orchestrated by several metabolic pathways and regulatory networks acting at the transcript,
protein, metabolite and physiological levels.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting
T1  - Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1847
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2022",
abstract = "Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study was to identify key candidates and pathways involved in desiccation
tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,
proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the
cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain
were performed. In total, 1192 different protein groups were quantified by TMT-based comparative
quantitative proteomics. Among them, 408 protein groups showed a statistically significant
difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the
majority of proteins related to photosynthetic processes were less abundant, while chlorophyll
fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic
electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione
cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were
reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins
(GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),
were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall
polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/
HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific
process orchestrated by several metabolic pathways and regulatory networks acting at the transcript,
protein, metabolite and physiological levels.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting",
title = "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1847"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2022). Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 57.
https://hdl.handle.net/21.15107/rcub_imagine_1847

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:57.
https://hdl.handle.net/21.15107/rcub_imagine_1847 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):57,
https://hdl.handle.net/21.15107/rcub_imagine_1847 .

TY  - CONF
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Milić, Dejana
AU  - Milić Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1846
AB  - An ancient resurrection plant Ramonda serbica Panc. is able to survive a long desiccation period
and reestablish metabolic activity upon watering. A hallmark of desiccation tolerance in the resurrection
species is the accumulation of protective late embryogenesis abundant proteins (LEAPs).
These intrinsically disordered proteins (IDPs) may stabilize the correct structure of proteins and
membranes during cellular dehydration. The aim of our study was to assess LEA genes’ expression
levels in hydrated (HL) and desiccated leaves (DL) and to identify, characterise, and estimate
the potential role of R. serbica LEAPs in desiccation tolerance. In total, 318 LEAPs from HL and DL
were identified and classified into the seven LEA protein family groups ranging from LEA1-LEA5,
seed maturation proteins (SMPs), and dehydrins (DEH). Analysis of the physicochemical properties,
motif architecture, secondary structure, homology, and phylogenetic relationships demonstrated
that R. serbica LEAPs greatly differed among the LEA family groups. The most abundant LEA2
proteins (mostly downregulated upon desiccation) exhibited lower hydrophilicity and propensity
to fold into organised globular domains. Oppositely, hydrophilic LEA4 proteins tended to form
amphipathic, A-type, α-helices. Most of desiccation-upregulated LEA genes encoded highly disordered
DEH1, LEA1, LEA4.2, and LEA4.3 proteins. While dehydrins might chelate metals and bind
DNA under water deficit, other ID LEAPs (e.g. LEA1, LEA3, LEA4) might participate in forming intracellular
proteinaceous condensates or adopt amphipathic α-helical conformation, enabling them
to stabilise desiccation-sensitive proteins and membranes. Taken together, possible functions of
LEAPs are discussed with significant implications on drought tolerance improvement of crops
grown in arid areas.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting
T1  - Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization
SP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1846
ER  - 

@conference{
author = "Pantelić, Ana and Stevanović, Strahinja and Milić, Dejana and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2022",
abstract = "An ancient resurrection plant Ramonda serbica Panc. is able to survive a long desiccation period
and reestablish metabolic activity upon watering. A hallmark of desiccation tolerance in the resurrection
species is the accumulation of protective late embryogenesis abundant proteins (LEAPs).
These intrinsically disordered proteins (IDPs) may stabilize the correct structure of proteins and
membranes during cellular dehydration. The aim of our study was to assess LEA genes’ expression
levels in hydrated (HL) and desiccated leaves (DL) and to identify, characterise, and estimate
the potential role of R. serbica LEAPs in desiccation tolerance. In total, 318 LEAPs from HL and DL
were identified and classified into the seven LEA protein family groups ranging from LEA1-LEA5,
seed maturation proteins (SMPs), and dehydrins (DEH). Analysis of the physicochemical properties,
motif architecture, secondary structure, homology, and phylogenetic relationships demonstrated
that R. serbica LEAPs greatly differed among the LEA family groups. The most abundant LEA2
proteins (mostly downregulated upon desiccation) exhibited lower hydrophilicity and propensity
to fold into organised globular domains. Oppositely, hydrophilic LEA4 proteins tended to form
amphipathic, A-type, α-helices. Most of desiccation-upregulated LEA genes encoded highly disordered
DEH1, LEA1, LEA4.2, and LEA4.3 proteins. While dehydrins might chelate metals and bind
DNA under water deficit, other ID LEAPs (e.g. LEA1, LEA3, LEA4) might participate in forming intracellular
proteinaceous condensates or adopt amphipathic α-helical conformation, enabling them
to stabilise desiccation-sensitive proteins and membranes. Taken together, possible functions of
LEAPs are discussed with significant implications on drought tolerance improvement of crops
grown in arid areas.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting",
title = "Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization",
pages = "95",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1846"
}

Pantelić, A., Stevanović, S., Milić, D., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2022). Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization. in 4th International Conference on Plant Biology and 23rd SPPS Meeting
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 95.
https://hdl.handle.net/21.15107/rcub_imagine_1846

Pantelić A, Stevanović S, Milić D, Milić Komić S, Kilibarda N, Vidović M. Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:95.
https://hdl.handle.net/21.15107/rcub_imagine_1846 .

Pantelić, Ana, Stevanović, Strahinja, Milić, Dejana, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):95,
https://hdl.handle.net/21.15107/rcub_imagine_1846 .

TY  - JOUR
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1604
AB  - The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO center dot activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study
IS  - 9
VL  - 11
DO  - 10.3390/plants11091199
ER  - 

@article{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO center dot activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study",
number = "9",
volume = "11",
doi = "10.3390/plants11091199"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović-Jovanović, S.. (2022). Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. in Plants-Basel
MDPI, Basel., 11(9).
https://doi.org/10.3390/plants11091199

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović-Jovanović S. Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. in Plants-Basel. 2022;11(9).
doi:10.3390/plants11091199 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović-Jovanović, Sonja, "Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study" in Plants-Basel, 11, no. 9 (2022),
https://doi.org/10.3390/plants11091199 . .

TY  - JOUR
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Komić, Sonja Milic
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1560
AB  - Ramonda serbica Panc. is an ancient resurrection plant able to survive a long desiccation period and recover metabolic functions upon watering. The accumulation of protective late embryogenesis abundant proteins (LEAPs) is a desiccation tolerance hallmark. To propose their role in R. serbica desiccation tolerance, we structurally characterised LEAPs and evaluated LEA gene expression levels in hydrated and desiccated leaves. By integrating de novo transcriptomics and homologues LEAP domains, 318 R. serbica LEAPs were identified and classified according to their conserved motifs and phylogeny. The in silico analysis revealed that hydrophilic LEA4 proteins exhibited an exceptionally high tendency to form amphipathic alpha-helices. The most abundant, atypical LEA2 group contained more hydrophobic proteins predicted to fold into the defined globular domains. Within the desiccation-upregulated LEA genes, the majority encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins, while the greatest portion of downregulated genes encoded LEA2.3 and LEA2.5 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other intrinsically disordered LEAPs might participate in forming intracellular proteinaceous condensates or adopt amphipathic alpha-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. This comprehensive LEAPs structural characterisation is essential to understanding their function and regulation during desiccation aiming at crop drought tolerance improvement.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc
IS  - 7
VL  - 23
DO  - 10.3390/ijms23073547
ER  - 

@article{
author = "Pantelić, Ana and Stevanović, Strahinja and Komić, Sonja Milic and Kilibarda, Nataša and Vidović, Marija",
year = "2022",
abstract = "Ramonda serbica Panc. is an ancient resurrection plant able to survive a long desiccation period and recover metabolic functions upon watering. The accumulation of protective late embryogenesis abundant proteins (LEAPs) is a desiccation tolerance hallmark. To propose their role in R. serbica desiccation tolerance, we structurally characterised LEAPs and evaluated LEA gene expression levels in hydrated and desiccated leaves. By integrating de novo transcriptomics and homologues LEAP domains, 318 R. serbica LEAPs were identified and classified according to their conserved motifs and phylogeny. The in silico analysis revealed that hydrophilic LEA4 proteins exhibited an exceptionally high tendency to form amphipathic alpha-helices. The most abundant, atypical LEA2 group contained more hydrophobic proteins predicted to fold into the defined globular domains. Within the desiccation-upregulated LEA genes, the majority encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins, while the greatest portion of downregulated genes encoded LEA2.3 and LEA2.5 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other intrinsically disordered LEAPs might participate in forming intracellular proteinaceous condensates or adopt amphipathic alpha-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. This comprehensive LEAPs structural characterisation is essential to understanding their function and regulation during desiccation aiming at crop drought tolerance improvement.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc",
number = "7",
volume = "23",
doi = "10.3390/ijms23073547"
}

Pantelić, A., Stevanović, S., Komić, S. M., Kilibarda, N.,& Vidović, M.. (2022). In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc. in International Journal of Molecular Sciences
MDPI, Basel., 23(7).
https://doi.org/10.3390/ijms23073547

Pantelić A, Stevanović S, Komić SM, Kilibarda N, Vidović M. In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc. in International Journal of Molecular Sciences. 2022;23(7).
doi:10.3390/ijms23073547 .

Pantelić, Ana, Stevanović, Strahinja, Komić, Sonja Milic, Kilibarda, Nataša, Vidović, Marija, "In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc" in International Journal of Molecular Sciences, 23, no. 7 (2022),
https://doi.org/10.3390/ijms23073547 . .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Veljković Jovanović, Sonja
AU  - Pantelić, Ana
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1775
AB  - Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter
a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica Panč. is a relic and endemic species
that belongs to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing
subject of many researchers. Most of the genes responsible for this amazing ability are present in other plants, and
research of those genes which could be activated in crops is growing much more attention because of the imminent
crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica
plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study.
Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation
tolerance. Defining LEAPs physicochemical characteristics and specific physiological functions may lead us to their
applicability in other areas of research.
PB  - Novi Sad : Faculty of Sciences, Department of Biology
T2  - Biologia Serbica
T1  - Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.
EP  - 66
IS  - 1
SP  - 59
VL  - 41
DO  - 10.5281/zenodo.7075212
ER  - 

@article{
author = "Milić Komić, Sonja and Veljković Jovanović, Sonja and Pantelić, Ana and Vidović, Marija",
year = "2022",
abstract = "Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter
a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica Panč. is a relic and endemic species
that belongs to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing
subject of many researchers. Most of the genes responsible for this amazing ability are present in other plants, and
research of those genes which could be activated in crops is growing much more attention because of the imminent
crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica
plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study.
Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation
tolerance. Defining LEAPs physicochemical characteristics and specific physiological functions may lead us to their
applicability in other areas of research.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology",
journal = "Biologia Serbica",
title = "Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.",
pages = "66-59",
number = "1",
volume = "41",
doi = "10.5281/zenodo.7075212"
}

Milić Komić, S., Veljković Jovanović, S., Pantelić, A.,& Vidović, M.. (2022). Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology., 41(1), 59-66.
https://doi.org/10.5281/zenodo.7075212

Milić Komić S, Veljković Jovanović S, Pantelić A, Vidović M. Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.. in Biologia Serbica. 2022;41(1):59-66.
doi:10.5281/zenodo.7075212 .

Milić Komić, Sonja, Veljković Jovanović, Sonja, Pantelić, Ana, Vidović, Marija, "Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč." in Biologia Serbica, 41, no. 1 (2022):59-66,
https://doi.org/10.5281/zenodo.7075212 . .

TY  - DATA
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Milić-Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1853
AB  - Ramonda serbica de novo transcriptome database
PB  - MDPI
T2  - International Journal of Molecular Science
T1  - Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves
VL  - n/a
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1853
ER  - 

@misc{
author = "Pantelić, Ana and Stevanović, Strahinja and Milić-Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2022",
abstract = "Ramonda serbica de novo transcriptome database",
publisher = "MDPI",
journal = "International Journal of Molecular Science",
title = "Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves",
volume = "n/a",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1853"
}

Pantelić, A., Stevanović, S., Milić-Komić, S., Kilibarda, N.,& Vidović, M.. (2022). Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves. in International Journal of Molecular Science
MDPI., n/a.
https://hdl.handle.net/21.15107/rcub_imagine_1853

Pantelić A, Stevanović S, Milić-Komić S, Kilibarda N, Vidović M. Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves. in International Journal of Molecular Science. 2022;n/a.
https://hdl.handle.net/21.15107/rcub_imagine_1853 .

Pantelić, Ana, Stevanović, Strahinja, Milić-Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves" in International Journal of Molecular Science, n/a (2022),
https://hdl.handle.net/21.15107/rcub_imagine_1853 .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1833
AB  - Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a longperiod and fully resume metabolic functions upon rewatering in a very short period, even within48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of thecell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chainwere performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significantdifference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, themajority of proteins related to photosynthetic processes were less abundant, while chlorophyllfluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclicelectron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) werereduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wallpolymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels.
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting
T1  - Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects
EP  - 27
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1833
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2022",
abstract = "Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a longperiod and fully resume metabolic functions upon rewatering in a very short period, even within48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of thecell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chainwere performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significantdifference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, themajority of proteins related to photosynthetic processes were less abundant, while chlorophyllfluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclicelectron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) werereduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wallpolymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels.",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting",
title = "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1833"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2022). Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 27-27.
https://hdl.handle.net/21.15107/rcub_imagine_1833

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:27-27.
https://hdl.handle.net/21.15107/rcub_imagine_1833 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):27-27,
https://hdl.handle.net/21.15107/rcub_imagine_1833 .

TY  - CONF
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2021
UR  - http://ojs.pmf.uns.ac.rs/index.php/dbe_serbica/index
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1856
AB  - An extreme loss of cellular water or desiccation (5-10% of relative water content) leads to protein denaturation, aggregation and degradation, and affects the fluidity of membrane lipids resulting in loss of membrane integrity [1]. The essential constituents of vegetative desiccation tolerance in so-called resurrection plants are late embryogenesis abundant proteins (LEAPs). This heterogeneous group of anhydrobiosis-related intrinsically disordered proteins forms mostly random conformation when fully hydrated, turning into compact α-helices during desiccation [2]. Based on in vitro studies, LEAPs can be involved in water binding, ion sequestration, stabilization of both membrane and enzymes during freezing or drying, while by forming intracellular proteinaceous condensates they increase structural integrity and intracellular viscosity of cells during desiccation.Here, we identify 164 members of LEA gene family in endemic and relict resurrection species Ramonda serbica by integrating previously done de novo transcriptome and homologues protein motifs. Identified LEAPs were classification into six groups according to Protein family (PFAM) database and the most populated group was LEA4 containing 47% of total identified LEAPs. By using four secondary structure predictors, we showed that this group exhibited a high propensity to form amphipathic α-helices (81% of total sequence length is predicted to form α-helical structure). This implies that charged residues might be exposed to the solvent, while hydrophobic amino acids might interact with lipid bilayers or with other target proteins in the cell. In addition, as predicted by several bioinformatic tools, more than 70% of identified LEAPs were found to be highly disordered (~64%). Structural characterization of LEAPs is a key to understand their function and regulation of their intrinsic structural disorder-to-order transition during desiccation. These findings will promote transformative advancements in various fields, such as the development of new strategies in neurodegenerative disorders, cell preservation technology and the improvement of crop drought tolerance.
PB  - Novi Sad : Faculty of Sciences, Department of Biology and Ecology
C3  - Biologia Serbica
T1  - De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins
EP  - 65
IS  - 1 (spec. ed.)
SP  - 65
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1856
ER  - 

@conference{
author = "Pantelić, Ana and Stevanović, Strahinja and Kilibarda, Nataša and Vidović, Marija",
year = "2021",
abstract = "An extreme loss of cellular water or desiccation (5-10% of relative water content) leads to protein denaturation, aggregation and degradation, and affects the fluidity of membrane lipids resulting in loss of membrane integrity [1]. The essential constituents of vegetative desiccation tolerance in so-called resurrection plants are late embryogenesis abundant proteins (LEAPs). This heterogeneous group of anhydrobiosis-related intrinsically disordered proteins forms mostly random conformation when fully hydrated, turning into compact α-helices during desiccation [2]. Based on in vitro studies, LEAPs can be involved in water binding, ion sequestration, stabilization of both membrane and enzymes during freezing or drying, while by forming intracellular proteinaceous condensates they increase structural integrity and intracellular viscosity of cells during desiccation.Here, we identify 164 members of LEA gene family in endemic and relict resurrection species Ramonda serbica by integrating previously done de novo transcriptome and homologues protein motifs. Identified LEAPs were classification into six groups according to Protein family (PFAM) database and the most populated group was LEA4 containing 47% of total identified LEAPs. By using four secondary structure predictors, we showed that this group exhibited a high propensity to form amphipathic α-helices (81% of total sequence length is predicted to form α-helical structure). This implies that charged residues might be exposed to the solvent, while hydrophobic amino acids might interact with lipid bilayers or with other target proteins in the cell. In addition, as predicted by several bioinformatic tools, more than 70% of identified LEAPs were found to be highly disordered (~64%). Structural characterization of LEAPs is a key to understand their function and regulation of their intrinsic structural disorder-to-order transition during desiccation. These findings will promote transformative advancements in various fields, such as the development of new strategies in neurodegenerative disorders, cell preservation technology and the improvement of crop drought tolerance.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology and Ecology",
journal = "Biologia Serbica",
title = "De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins",
pages = "65-65",
number = "1 (spec. ed.)",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1856"
}

Pantelić, A., Stevanović, S., Kilibarda, N.,& Vidović, M.. (2021). De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology and Ecology., 43(1 (spec. ed.)), 65-65.
https://hdl.handle.net/21.15107/rcub_imagine_1856

Pantelić A, Stevanović S, Kilibarda N, Vidović M. De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins. in Biologia Serbica. 2021;43(1 (spec. ed.)):65-65.
https://hdl.handle.net/21.15107/rcub_imagine_1856 .

Pantelić, Ana, Stevanović, Strahinja, Kilibarda, Nataša, Vidović, Marija, "De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins" in Biologia Serbica, 43, no. 1 (spec. ed.) (2021):65-65,
https://hdl.handle.net/21.15107/rcub_imagine_1856 .

TY  - CONF
AU  - Vidović, Marija
AU  - Stevanović, Strahinja
AU  - Pantelić, Ana
AU  - Veljović-Jovanović, Sonja
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1855
AB  - Ramonda serbica Panc. is a resurrection plant that can survive a long period of severe dehydrationdesiccation.Desiccation induces cellular membrane integrity loss, protein aggregation, and denaturation, as well asaccelerated generation of reactive oxygen species. However, R. serbica can fully recover its metabolic functionsalready one day upon watering [1].Aim: to obtain more insight into the mechanisms of desiccation tolerance in R. serbica by differential de novotranscriptomics of hydrated (HL) and desiccated leaves (DL).
C3  - Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia
T1  - De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1855
ER  - 

@conference{
author = "Vidović, Marija and Stevanović, Strahinja and Pantelić, Ana and Veljović-Jovanović, Sonja",
year = "2021",
abstract = "Ramonda serbica Panc. is a resurrection plant that can survive a long period of severe dehydrationdesiccation.Desiccation induces cellular membrane integrity loss, protein aggregation, and denaturation, as well asaccelerated generation of reactive oxygen species. However, R. serbica can fully recover its metabolic functionsalready one day upon watering [1].Aim: to obtain more insight into the mechanisms of desiccation tolerance in R. serbica by differential de novotranscriptomics of hydrated (HL) and desiccated leaves (DL).",
journal = "Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia",
title = "De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1855"
}

Vidović, M., Stevanović, S., Pantelić, A.,& Veljović-Jovanović, S.. (2021). De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance. in Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia.
https://hdl.handle.net/21.15107/rcub_imagine_1855

Vidović M, Stevanović S, Pantelić A, Veljović-Jovanović S. De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance. in Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia. 2021;.
https://hdl.handle.net/21.15107/rcub_imagine_1855 .

Vidović, Marija, Stevanović, Strahinja, Pantelić, Ana, Veljović-Jovanović, Sonja, "De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance" in Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia (2021),
https://hdl.handle.net/21.15107/rcub_imagine_1855 .