TY  - DATA
AU  - Pantelić, Brana
AU  - Škaro Bogojević, Sanja
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Lončarević, Branka
AU  - Beskoski, Vladimir
AU  - Maslak, Veselin
AU  - Guzik, Maciej
AU  - Makryniotis, Konstantinos
AU  - Taxeidis, George
AU  - Siaperas, Romanos
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2073-4344/13/2/278
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1792
T2  - Catalysts
T1  - Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278
IS  - 2
SP  - 278
VL  - 13
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1792
ER  - 

@misc{
author = "Pantelić, Brana and Škaro Bogojević, Sanja and Milivojević, Dušan and Ilić-Tomić, Tatjana and Lončarević, Branka and Beskoski, Vladimir and Maslak, Veselin and Guzik, Maciej and Makryniotis, Konstantinos and Taxeidis, George and Siaperas, Romanos and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
journal = "Catalysts",
title = "Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278",
number = "2",
pages = "278",
volume = "13",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1792"
}

Pantelić, B., Škaro Bogojević, S., Milivojević, D., Ilić-Tomić, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E.,& Nikodinović-Runić, J.. (2023). Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278. in Catalysts, 13(2), 278.
https://hdl.handle.net/21.15107/rcub_imagine_1792

Pantelić B, Škaro Bogojević S, Milivojević D, Ilić-Tomić T, Lončarević B, Beskoski V, Maslak V, Guzik M, Makryniotis K, Taxeidis G, Siaperas R, Topakas E, Nikodinović-Runić J. Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278. in Catalysts. 2023;13(2):278.
https://hdl.handle.net/21.15107/rcub_imagine_1792 .

Pantelić, Brana, Škaro Bogojević, Sanja, Milivojević, Dušan, Ilić-Tomić, Tatjana, Lončarević, Branka, Beskoski, Vladimir, Maslak, Veselin, Guzik, Maciej, Makryniotis, Konstantinos, Taxeidis, George, Siaperas, Romanos, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278" in Catalysts, 13, no. 2 (2023):278,
https://hdl.handle.net/21.15107/rcub_imagine_1792 .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Škaro Bogojević, Sanja
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Lončarević, Branka
AU  - Beskoski, Vladimir
AU  - Maslak, Veselin
AU  - Guzik, Maciej
AU  - Makryniotis, Konstantinos
AU  - Taxeidis, George
AU  - Siaperas, Romanos
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2073-4344/13/2/278
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1790
AB  - Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.
T2  - Catalysts
T2  - Catalysts
T1  - Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts
IS  - 2
SP  - 278
VL  - 13
DO  - 10.3390/catal13020278
ER  - 

@article{
author = "Pantelić, Brana and Škaro Bogojević, Sanja and Milivojević, Dušan and Ilić-Tomić, Tatjana and Lončarević, Branka and Beskoski, Vladimir and Maslak, Veselin and Guzik, Maciej and Makryniotis, Konstantinos and Taxeidis, George and Siaperas, Romanos and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.",
journal = "Catalysts, Catalysts",
title = "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts",
number = "2",
pages = "278",
volume = "13",
doi = "10.3390/catal13020278"
}

Pantelić, B., Škaro Bogojević, S., Milivojević, D., Ilić-Tomić, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E.,& Nikodinović-Runić, J.. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts, 13(2), 278.
https://doi.org/10.3390/catal13020278

Pantelić B, Škaro Bogojević S, Milivojević D, Ilić-Tomić T, Lončarević B, Beskoski V, Maslak V, Guzik M, Makryniotis K, Taxeidis G, Siaperas R, Topakas E, Nikodinović-Runić J. Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts. 2023;13(2):278.
doi:10.3390/catal13020278 .

Pantelić, Brana, Škaro Bogojević, Sanja, Milivojević, Dušan, Ilić-Tomić, Tatjana, Lončarević, Branka, Beskoski, Vladimir, Maslak, Veselin, Guzik, Maciej, Makryniotis, Konstantinos, Taxeidis, George, Siaperas, Romanos, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts" in Catalysts, 13, no. 2 (2023):278,
https://doi.org/10.3390/catal13020278 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Soković Bajić, Svetlana
AU  - Jovanović, Živko
AU  - Matić, Ivana
AU  - Tolinački, Maja
AU  - Popović, Dušanka
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Beskoski, Vladimir
AU  - Samardžić, Jelena
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1337
AB  - In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix
VL  - 11
DO  - 10.3389/fmicb.2020.601616
ER  - 

@article{
author = "Stanisavljević, Nemanja and Soković Bajić, Svetlana and Jovanović, Živko and Matić, Ivana and Tolinački, Maja and Popović, Dušanka and Popović, Nikola and Terzić-Vidojević, Amarela and Golić, Nataša and Beskoski, Vladimir and Samardžić, Jelena",
year = "2020",
abstract = "In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix",
volume = "11",
doi = "10.3389/fmicb.2020.601616"
}

Stanisavljević, N., Soković Bajić, S., Jovanović, Ž., Matić, I., Tolinački, M., Popović, D., Popović, N., Terzić-Vidojević, A., Golić, N., Beskoski, V.,& Samardžić, J.. (2020). Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 11.
https://doi.org/10.3389/fmicb.2020.601616

Stanisavljević N, Soković Bajić S, Jovanović Ž, Matić I, Tolinački M, Popović D, Popović N, Terzić-Vidojević A, Golić N, Beskoski V, Samardžić J. Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology. 2020;11.
doi:10.3389/fmicb.2020.601616 .

Stanisavljević, Nemanja, Soković Bajić, Svetlana, Jovanović, Živko, Matić, Ivana, Tolinački, Maja, Popović, Dušanka, Popović, Nikola, Terzić-Vidojević, Amarela, Golić, Nataša, Beskoski, Vladimir, Samardžić, Jelena, "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix" in Frontiers in Microbiology, 11 (2020),
https://doi.org/10.3389/fmicb.2020.601616 . .