TY  - CONF
AU  - Galić, Ivana
AU  - Bez, Christina
AU  - Bertani, Iris
AU  - Simić, Milena
AU  - Venturi, Vittorio
AU  - Stanković, Nada
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2370
AB  - The soil microbiome controls key functions in
agroecosystems determining soil fertility, crop
productivity, and stress tolerance. Crop rotation
is one of the oldest agricultural practices that has
a positive effect on soil quality and the control of
weeds, pests, and pathogens. New insights into
soil microbiome confirm the positive influence of
crop rotation on the diversity of the microbiome.
This study investigates the effects of different
crop rotations under conventional fertilization/
weed management practices on soil microbial
diversity and community structure in two of the
most commonly grown crops in Serbia. The study
investigated the bacterial population in samples
from long-term cultivation experiment of maize
continuous cropping (M-CC) and maize-winter
wheat rotation (M-WW) at two-time points
(December 2022 and May 2023). The results of
16S rDNA amplicon community profiling and
beta diversity analysis showed clear clustering
depending on season, cropping sequence, and
herbicide application. The results show that the
composition of the bacterial community in soil
is largely influenced by seasonal changes. When
comparing bacterial communities in the same
season (May), we observed a significantly higher
biodiversity in M-CC compared to M-WW soils,
suggesting that type of agricultural practice has
a significant impact on the composition of the
microbiome by influencing selection and survival
of specific microbial taxa. In addition, a significant
shift was observed between the bacterial
community composition in M-CC samples under
herbicide treatment and in control soils in December,
while the M-WW community was stable
under all conditions tested. Taxonomically, Actinobacteria
dominated the soil microbiome under
all conditions (53%), followed by Proteobacteria
(23%), Acidobacteria (15%), and Firmicutes (8%).
Despite fluctuations in relative abundance, some
interesting taxa, including Bacillus, Microlunatus,
and Blastococcus, dominated the soil microbiome
under all conditions. These data provide insights
into microbial dynamics, integrating the cultivation
methods with metagenomic approaches to
evaluate different cropping practices.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION
EP  - 69
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2370
ER  - 

@conference{
author = "Galić, Ivana and Bez, Christina and Bertani, Iris and Simić, Milena and Venturi, Vittorio and Stanković, Nada",
year = "2024",
abstract = "The soil microbiome controls key functions in
agroecosystems determining soil fertility, crop
productivity, and stress tolerance. Crop rotation
is one of the oldest agricultural practices that has
a positive effect on soil quality and the control of
weeds, pests, and pathogens. New insights into
soil microbiome confirm the positive influence of
crop rotation on the diversity of the microbiome.
This study investigates the effects of different
crop rotations under conventional fertilization/
weed management practices on soil microbial
diversity and community structure in two of the
most commonly grown crops in Serbia. The study
investigated the bacterial population in samples
from long-term cultivation experiment of maize
continuous cropping (M-CC) and maize-winter
wheat rotation (M-WW) at two-time points
(December 2022 and May 2023). The results of
16S rDNA amplicon community profiling and
beta diversity analysis showed clear clustering
depending on season, cropping sequence, and
herbicide application. The results show that the
composition of the bacterial community in soil
is largely influenced by seasonal changes. When
comparing bacterial communities in the same
season (May), we observed a significantly higher
biodiversity in M-CC compared to M-WW soils,
suggesting that type of agricultural practice has
a significant impact on the composition of the
microbiome by influencing selection and survival
of specific microbial taxa. In addition, a significant
shift was observed between the bacterial
community composition in M-CC samples under
herbicide treatment and in control soils in December,
while the M-WW community was stable
under all conditions tested. Taxonomically, Actinobacteria
dominated the soil microbiome under
all conditions (53%), followed by Proteobacteria
(23%), Acidobacteria (15%), and Firmicutes (8%).
Despite fluctuations in relative abundance, some
interesting taxa, including Bacillus, Microlunatus,
and Blastococcus, dominated the soil microbiome
under all conditions. These data provide insights
into microbial dynamics, integrating the cultivation
methods with metagenomic approaches to
evaluate different cropping practices.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2370"
}

Galić, I., Bez, C., Bertani, I., Simić, M., Venturi, V.,& Stanković, N.. (2024). SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2370

Galić I, Bez C, Bertani I, Simić M, Venturi V, Stanković N. SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2370 .

Galić, Ivana, Bez, Christina, Bertani, Iris, Simić, Milena, Venturi, Vittorio, Stanković, Nada, "SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):69-69,
https://hdl.handle.net/21.15107/rcub_imagine_2370 .

TY  - JOUR
AU  - Dragojević, Milica
AU  - Stanković, Nada
AU  - Đokić, Lidija
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
PY  - 2023
UR  - https://doi.org/10.1186/s40793-023-00477-x
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1796
AB  - The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used to analyze the bacterial communities in the endorizosphere of indigenous succulent halophytes Salicornia europaea, Suaeda maritima, and Camphorosma annua from the natural salt marshes of Slano Kopovo (Serbia). The 16 S rDNA analyses gave, for the first time, an insight into the composition of the endophytic bacterial communities of S. maritima and C. annua. We have found that the composition of endophyte microbiomes in the same habitat is to some extent influenced by plant species. A cultivable portion of the halophyte microbiota was tested at different NaCl concentrations for the set of plant growth promoting (PGP) traits. Through the mining of indigenous halotolerant endophytes, we obtained a collection representing a core endophyte microbiome conferring desirable PGP traits. The majority (65%) of the selected strains belonged to the common halotolerant/halophilic genera Halomonas, Kushneria, and Halobacillus, with representatives exhibiting multiple PGP traits, and retaining beneficial traits in conditions of the increased salinity. The results suggest that the root endosphere of halophytes is a valuable source of PGP bacteria supporting plant growth and fitness in salt-affected soils.
T2  - Environmental Microbiome
T1  - Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria
IS  - 1
SP  - 20
VL  - 18
DO  - 10.1186/s40793-023-00477-x
ER  - 

@article{
author = "Dragojević, Milica and Stanković, Nada and Đokić, Lidija and Raičević, Vera and Jovičić-Petrović, Jelena",
year = "2023",
abstract = "The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used to analyze the bacterial communities in the endorizosphere of indigenous succulent halophytes Salicornia europaea, Suaeda maritima, and Camphorosma annua from the natural salt marshes of Slano Kopovo (Serbia). The 16 S rDNA analyses gave, for the first time, an insight into the composition of the endophytic bacterial communities of S. maritima and C. annua. We have found that the composition of endophyte microbiomes in the same habitat is to some extent influenced by plant species. A cultivable portion of the halophyte microbiota was tested at different NaCl concentrations for the set of plant growth promoting (PGP) traits. Through the mining of indigenous halotolerant endophytes, we obtained a collection representing a core endophyte microbiome conferring desirable PGP traits. The majority (65%) of the selected strains belonged to the common halotolerant/halophilic genera Halomonas, Kushneria, and Halobacillus, with representatives exhibiting multiple PGP traits, and retaining beneficial traits in conditions of the increased salinity. The results suggest that the root endosphere of halophytes is a valuable source of PGP bacteria supporting plant growth and fitness in salt-affected soils.",
journal = "Environmental Microbiome",
title = "Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria",
number = "1",
pages = "20",
volume = "18",
doi = "10.1186/s40793-023-00477-x"
}

Dragojević, M., Stanković, N., Đokić, L., Raičević, V.,& Jovičić-Petrović, J.. (2023). Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria. in Environmental Microbiome, 18(1), 20.
https://doi.org/10.1186/s40793-023-00477-x

Dragojević M, Stanković N, Đokić L, Raičević V, Jovičić-Petrović J. Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria. in Environmental Microbiome. 2023;18(1):20.
doi:10.1186/s40793-023-00477-x .

Dragojević, Milica, Stanković, Nada, Đokić, Lidija, Raičević, Vera, Jovičić-Petrović, Jelena, "Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria" in Environmental Microbiome, 18, no. 1 (2023):20,
https://doi.org/10.1186/s40793-023-00477-x . .

TY  - JOUR
AU  - Joncić Savić, Katarina
AU  - Đokic, Lidija
AU  - Stanković, Nada
AU  - Morić, Ivana
AU  - Pavlović, Bojan
AU  - Šenerović, Lidija
AU  - Aydogan, Cem
AU  - Pavić, Aleksandar
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S1756464623002177
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1982
AB  - Candidiasis caused by Candida albicans is one of the most common fungal infections in modern society. The limited arsenal of clinical drugs, their side effects and emerging resistance, largely contribute to the low efficacy of current antifungal therapies. The morphogenetic yeast-to-hyphae transition is the key virulence feature for the establishment of local and systemic C. albicans infections. We show that a delfinidin-rich fruit extract from Aristotelia chilensis ([Molina], Stuntz) (maqui berry) inhibits filamentation of C. albicans, in both laboratory and clinical strains. The extract acts synergistically with nystatin, with filament formation completely prevented by the combination of ¼×MIC of nystatin and 0.125 mg/ml of the extract. The combination treatment results in increased survival of C. albicans-infected zebrafish embryos compared to treatment with nystatin. Neither the extract nor its combination with nystatin was toxic at effective doses. These results warrant further investigation of maqui berry extract as adjuvant antifungal treatment.
T2  - Journal of Functional Foods
T1  - Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin
SP  - 105617
VL  - 106
DO  - 10.1016/j.jff.2023.105617
ER  - 

@article{
author = "Joncić Savić, Katarina and Đokic, Lidija and Stanković, Nada and Morić, Ivana and Pavlović, Bojan and Šenerović, Lidija and Aydogan, Cem and Pavić, Aleksandar",
year = "2023",
abstract = "Candidiasis caused by Candida albicans is one of the most common fungal infections in modern society. The limited arsenal of clinical drugs, their side effects and emerging resistance, largely contribute to the low efficacy of current antifungal therapies. The morphogenetic yeast-to-hyphae transition is the key virulence feature for the establishment of local and systemic C. albicans infections. We show that a delfinidin-rich fruit extract from Aristotelia chilensis ([Molina], Stuntz) (maqui berry) inhibits filamentation of C. albicans, in both laboratory and clinical strains. The extract acts synergistically with nystatin, with filament formation completely prevented by the combination of ¼×MIC of nystatin and 0.125 mg/ml of the extract. The combination treatment results in increased survival of C. albicans-infected zebrafish embryos compared to treatment with nystatin. Neither the extract nor its combination with nystatin was toxic at effective doses. These results warrant further investigation of maqui berry extract as adjuvant antifungal treatment.",
journal = "Journal of Functional Foods",
title = "Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin",
pages = "105617",
volume = "106",
doi = "10.1016/j.jff.2023.105617"
}

Joncić Savić, K., Đokic, L., Stanković, N., Morić, I., Pavlović, B., Šenerović, L., Aydogan, C.,& Pavić, A.. (2023). Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin. in Journal of Functional Foods, 106, 105617.
https://doi.org/10.1016/j.jff.2023.105617

Joncić Savić K, Đokic L, Stanković N, Morić I, Pavlović B, Šenerović L, Aydogan C, Pavić A. Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin. in Journal of Functional Foods. 2023;106:105617.
doi:10.1016/j.jff.2023.105617 .

Joncić Savić, Katarina, Đokic, Lidija, Stanković, Nada, Morić, Ivana, Pavlović, Bojan, Šenerović, Lidija, Aydogan, Cem, Pavić, Aleksandar, "Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin" in Journal of Functional Foods, 106 (2023):105617,
https://doi.org/10.1016/j.jff.2023.105617 . .

TY  - CONF
AU  - Dragojević, Milica
AU  - Đokić, Lidija
AU  - Stanković, Nada
AU  - Raičević, Vera
AU  - Karličić, Vera
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2160
AB  - soil salinity has numerous negative effects on plant growth and poses
a significant threat to agricultural productivity worldwide. The diversity of
endophytic bacterial communities found in various indigenous halophytes
such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may
reveal beneficial bacteria with various plant growthpromoting (PGP) properties.
Members of Halomonas, the largest genus of the family Halomonadaceae,
are rod-shaped, gram-negative, moderately halophilic bacteria that have been
increasingly isolated from various saline environments, including halophyte
tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting
endophytic bacteria can contribute to plant host growth, productivity, and
fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria
represents a good alternative strategy to mitigate the effects of salt stress in
crops and to recover saline soils. The aim of this study was to isolate halotolerant
bacteria from halophyte roots, and evaluate their PGP ability and their effect on
germination parameters of selected crops. The endophytic bacterial communities
in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia)
were analyzed using culture-dependent techniques. The genus Halomonas was
the predominant among the halotolerant root-associated bacteria of analyzed
halophytes. We have isolated seven Halomonas sp. strains, six of which were
closely related (>99,86% similarity) to Halomonas songnenensis, a moderately
halophilic bacterium from saline and alkaline soils. Obtained isolates were tested
at different sodium chloride concentrations for a range of PGP traits at
different concentrations of sodium chloride. The selected strains were used to
inoculate seeds iand monitor of the germination process of barley, sunflower,
and wheat under conditions of elevated salinity. Most of the isolates tolerate
the NaCl concentration up to 18% and showed multiple PGP features with some
of them retained even in the presence of additional salt concentrations. The
germination test results showed that Halomonas seed inoculation positively
affected barley germination parameters in conditions with and without added
salt. The observed traits can be further exploited to improve plant growth and
augment the salt tolerance of agricultural production on saline soils.
PB  - Belgrade : Faculty of Biology
C3  - ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
T1  - Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity
EP  - 71
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2160
ER  - 

@conference{
author = "Dragojević, Milica and Đokić, Lidija and Stanković, Nada and Raičević, Vera and Karličić, Vera and Lalević, Blažo and Jovičić-Petrović, Jelena",
year = "2023",
abstract = "soil salinity has numerous negative effects on plant growth and poses
a significant threat to agricultural productivity worldwide. The diversity of
endophytic bacterial communities found in various indigenous halophytes
such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may
reveal beneficial bacteria with various plant growthpromoting (PGP) properties.
Members of Halomonas, the largest genus of the family Halomonadaceae,
are rod-shaped, gram-negative, moderately halophilic bacteria that have been
increasingly isolated from various saline environments, including halophyte
tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting
endophytic bacteria can contribute to plant host growth, productivity, and
fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria
represents a good alternative strategy to mitigate the effects of salt stress in
crops and to recover saline soils. The aim of this study was to isolate halotolerant
bacteria from halophyte roots, and evaluate their PGP ability and their effect on
germination parameters of selected crops. The endophytic bacterial communities
in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia)
were analyzed using culture-dependent techniques. The genus Halomonas was
the predominant among the halotolerant root-associated bacteria of analyzed
halophytes. We have isolated seven Halomonas sp. strains, six of which were
closely related (>99,86% similarity) to Halomonas songnenensis, a moderately
halophilic bacterium from saline and alkaline soils. Obtained isolates were tested
at different sodium chloride concentrations for a range of PGP traits at
different concentrations of sodium chloride. The selected strains were used to
inoculate seeds iand monitor of the germination process of barley, sunflower,
and wheat under conditions of elevated salinity. Most of the isolates tolerate
the NaCl concentration up to 18% and showed multiple PGP features with some
of them retained even in the presence of additional salt concentrations. The
germination test results showed that Halomonas seed inoculation positively
affected barley germination parameters in conditions with and without added
salt. The observed traits can be further exploited to improve plant growth and
augment the salt tolerance of agricultural production on saline soils.",
publisher = "Belgrade : Faculty of Biology",
journal = "ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture",
title = "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2160"
}

Dragojević, M., Đokić, L., Stanković, N., Raičević, V., Karličić, V., Lalević, B.,& Jovičić-Petrović, J.. (2023). Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
Belgrade : Faculty of Biology., 71-71.
https://hdl.handle.net/21.15107/rcub_imagine_2160

Dragojević M, Đokić L, Stanković N, Raičević V, Karličić V, Lalević B, Jovičić-Petrović J. Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture. 2023;:71-71.
https://hdl.handle.net/21.15107/rcub_imagine_2160 .

Dragojević, Milica, Đokić, Lidija, Stanković, Nada, Raičević, Vera, Karličić, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity" in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture (2023):71-71,
https://hdl.handle.net/21.15107/rcub_imagine_2160 .

TY  - CONF
AU  - Galić, Ivana
AU  - Bertani, Iris
AU  - Bez, Cristina
AU  - Stanković, Nada
AU  - Venturi, Vittorio
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2159
AB  - Modern agriculture relies heavily on agrochemicals that can adversely affect
non-target organisms, including microorganisms. In terms of agricultural sustainability,
herbicideresilient plant growth-promoting (PGP) bacteria that can
improve crop yield are critical. Previously, we isolated 122 high herbicide concentration
tolerant (HHCT) bacteria from soils contaminated with S-metolachlor,
a widely used herbicide. Initially, in vitro experiments were conducted to screen
potential candidates for further evaluation. Our goal was to identify HHCT bacteria
with high PGP potential, and the ability to improve soil quality and biocontrol
activity. Subsequently, the most promising candidates were grouped
into three consortia and evaluated in pot for their effects on corn seed germination,
early-stage plant development, and root colonization. Experiments were
conducted with seeds of commercial maize hybrids under controlled conditions,
with and without the presence of the herbicide S-metolachlor. The results
showed that the consortia positively affected seed germination and promoted
higher germination rates. The bacterial isolates showed the ability to colonize
the root system of maize, with significant effects of the consortia on shoot and
root biomass and shoot and root length. However, it was observed in some
consortia that the presence of herbicides resulted in plant damage. Markedly,
the presence of the herbicide S-metolachlor reduced or reversed the beneficial
effects of bacterial consortia. This could be due to incomplete biotransformation
of the herbicide leading to the formation of toxic intermediates. The selection
of bacterial consortia that not only promote plant growth but also have the ability
to fully biotransform herbicides is critical. In conclusion, the isolated HHCT
bacteria showed promising PGP potential and formed an effective consortium
to promote maize seed germination and early-stage development. However,
careful consideration of the selection of bacterial consortia is required to ensure
their compatibility with herbicides and to minimize negative impacts on plant
health and environmental sustainability.
PB  - Belgrade : Faculty of Biology
C3  - ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
T1  - Herbicide-resilient bacterial consortia in plant growth promotion
EP  - 69
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2159
ER  - 

@conference{
author = "Galić, Ivana and Bertani, Iris and Bez, Cristina and Stanković, Nada and Venturi, Vittorio",
year = "2023",
abstract = "Modern agriculture relies heavily on agrochemicals that can adversely affect
non-target organisms, including microorganisms. In terms of agricultural sustainability,
herbicideresilient plant growth-promoting (PGP) bacteria that can
improve crop yield are critical. Previously, we isolated 122 high herbicide concentration
tolerant (HHCT) bacteria from soils contaminated with S-metolachlor,
a widely used herbicide. Initially, in vitro experiments were conducted to screen
potential candidates for further evaluation. Our goal was to identify HHCT bacteria
with high PGP potential, and the ability to improve soil quality and biocontrol
activity. Subsequently, the most promising candidates were grouped
into three consortia and evaluated in pot for their effects on corn seed germination,
early-stage plant development, and root colonization. Experiments were
conducted with seeds of commercial maize hybrids under controlled conditions,
with and without the presence of the herbicide S-metolachlor. The results
showed that the consortia positively affected seed germination and promoted
higher germination rates. The bacterial isolates showed the ability to colonize
the root system of maize, with significant effects of the consortia on shoot and
root biomass and shoot and root length. However, it was observed in some
consortia that the presence of herbicides resulted in plant damage. Markedly,
the presence of the herbicide S-metolachlor reduced or reversed the beneficial
effects of bacterial consortia. This could be due to incomplete biotransformation
of the herbicide leading to the formation of toxic intermediates. The selection
of bacterial consortia that not only promote plant growth but also have the ability
to fully biotransform herbicides is critical. In conclusion, the isolated HHCT
bacteria showed promising PGP potential and formed an effective consortium
to promote maize seed germination and early-stage development. However,
careful consideration of the selection of bacterial consortia is required to ensure
their compatibility with herbicides and to minimize negative impacts on plant
health and environmental sustainability.",
publisher = "Belgrade : Faculty of Biology",
journal = "ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture",
title = "Herbicide-resilient bacterial consortia in plant growth promotion",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2159"
}

Galić, I., Bertani, I., Bez, C., Stanković, N.,& Venturi, V.. (2023). Herbicide-resilient bacterial consortia in plant growth promotion. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
Belgrade : Faculty of Biology., 69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2159

Galić I, Bertani I, Bez C, Stanković N, Venturi V. Herbicide-resilient bacterial consortia in plant growth promotion. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture. 2023;:69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2159 .

Galić, Ivana, Bertani, Iris, Bez, Cristina, Stanković, Nada, Venturi, Vittorio, "Herbicide-resilient bacterial consortia in plant growth promotion" in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture (2023):69-69,
https://hdl.handle.net/21.15107/rcub_imagine_2159 .

TY  - CONF
AU  - Bez, Cristina
AU  - Galić, Ivana
AU  - Bertani, Iris
AU  - Stanković, Nada
AU  - Venturi, Vittorio
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2012
AB  - In terms of agricultural sustainability, herbicide-resistant, plant growth promoting (PGP)
bacteria that can improve crop yield are valuable resource. To exhibit PGP traits, the
bacteria must be able to colonize and survive in the rhizosphere.
Upon screening the herbicide-resistant bacterial collection, candidates with the highest
PGP potential were grouped into three consortia to evaluate their ability to colonize roots
and persist in the natural/local plant microbiome in the pot. Experiments were conducted
with seeds of commercial maize hybrids under controlled conditions, with and without
herbicide. Colonization ability was evaluated by examining multiple plants from each
treatment at two-time points during the experiment. 16S rRNA amplicon community
profiling was performed to precisely target the bacterial strains used in the three
consortia and investigate how the local microbiome might be altered by the application of
the consortia. Bioinformatic analysis was performed using qiime2, clustering of reads into
amplicon sequence variants ASVs using the DADA2 plugin, and the taxonomic assignment
was based on a customized dataset formed from the 16S rRNA gene sequences of the
ten isolates used in this study or by using the Silva rRNA database. For clustering and
comparison of ASVs based on sequence similarity, the program cd-hit was used, with
the sequence similarity parameter set to 98% to be considered part of the same cluster.
The obtained dataset was imported into R using the package qiime2R, and subsequent
analyzes and graphs were generated using either the R packages phyloseq, microbiome,
or reshape2. We identified seven out of ten inoculated strains in both time points tested
and with comparable abundance, indicating that most of the bacterial isolates tested have
the ability to colonize the root system of maize. Furthermore, the natural/local microbiome
of maize plants is not disturbed by the three consortia used in this study, implying that
they are good candidates for future biotechnological applications.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding
EP  - 71
SP  - 71
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2012
ER  - 

@conference{
author = "Bez, Cristina and Galić, Ivana and Bertani, Iris and Stanković, Nada and Venturi, Vittorio",
year = "2023",
abstract = "In terms of agricultural sustainability, herbicide-resistant, plant growth promoting (PGP)
bacteria that can improve crop yield are valuable resource. To exhibit PGP traits, the
bacteria must be able to colonize and survive in the rhizosphere.
Upon screening the herbicide-resistant bacterial collection, candidates with the highest
PGP potential were grouped into three consortia to evaluate their ability to colonize roots
and persist in the natural/local plant microbiome in the pot. Experiments were conducted
with seeds of commercial maize hybrids under controlled conditions, with and without
herbicide. Colonization ability was evaluated by examining multiple plants from each
treatment at two-time points during the experiment. 16S rRNA amplicon community
profiling was performed to precisely target the bacterial strains used in the three
consortia and investigate how the local microbiome might be altered by the application of
the consortia. Bioinformatic analysis was performed using qiime2, clustering of reads into
amplicon sequence variants ASVs using the DADA2 plugin, and the taxonomic assignment
was based on a customized dataset formed from the 16S rRNA gene sequences of the
ten isolates used in this study or by using the Silva rRNA database. For clustering and
comparison of ASVs based on sequence similarity, the program cd-hit was used, with
the sequence similarity parameter set to 98% to be considered part of the same cluster.
The obtained dataset was imported into R using the package qiime2R, and subsequent
analyzes and graphs were generated using either the R packages phyloseq, microbiome,
or reshape2. We identified seven out of ten inoculated strains in both time points tested
and with comparable abundance, indicating that most of the bacterial isolates tested have
the ability to colonize the root system of maize. Furthermore, the natural/local microbiome
of maize plants is not disturbed by the three consortia used in this study, implying that
they are good candidates for future biotechnological applications.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding",
pages = "71-71",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2012"
}

Bez, C., Galić, I., Bertani, I., Stanković, N.,& Venturi, V.. (2023). Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 71-71.
https://hdl.handle.net/21.15107/rcub_imagine_2012

Bez C, Galić I, Bertani I, Stanković N, Venturi V. Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding. in 4th Belgrade Bioinformatics Conference. 2023;4:71-71.
https://hdl.handle.net/21.15107/rcub_imagine_2012 .

Bez, Cristina, Galić, Ivana, Bertani, Iris, Stanković, Nada, Venturi, Vittorio, "Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding" in 4th Belgrade Bioinformatics Conference, 4 (2023):71-71,
https://hdl.handle.net/21.15107/rcub_imagine_2012 .

TY  - CONF
AU  - Galić, Ivana
AU  - Bertani, Iris
AU  - Bez, Cristina
AU  - Stanković, Nada
AU  - Venturi, Vittorio
PY  - 2023
UR  - https://ceesme.ecolres.hu/CEESME2023_abstract_book_v1.pdf
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2163
AB  - organisms, including microorganisms. In the global effort to increase agriculture sustainability, there
is an emerging need for herbicide-resilient plant growth-promoting (PGP) bacteria that improve
essential crops yield. From S-metolachlor-contaminated soils, we isolated 122 strains of high herbicide
concentration tolerant (HHCT) bacteria capable of growing on or using herbicide as their sole carbon
source. The aim of our study was to identify HHCT bacteria with the highest PGP potential, soil quality
improvement ability, biocontrol activity, and plant colonization capability; then combine them into
consortia that include all these traits, and test them in planta. PGP property evaluation included assays
for nitrogen fixation, phosphate solubilization, IAA, siderophore, and exopolysaccharide production,
detection of extracellular enzymatic activities, and motility. Strains were identified through 16S rDNA
sequencing. Biocontrol potential was evaluated against plant pathogens such as Fusarium sp. , and P.
fuscovaginae. The most promising candidates were combined in three consortia for growth-chamber
experiments to investigate the promotion of maize seed germination, early-stage plant development,
and root colonization evaluated by metagenomic analysis. The HHCT bacteria showed promising
PGP potential. 32% of the collection exhibited 3-4 of four tested direct PGP mechanisms. 22 isolates
had antifungal, and 13 had antibacterial activity. The strains with the highest in vitro PGP activity
predominantly belonged to Serratia, Pseudomonas, and Sphingobacterium genera. These strains
formed the most effective consortium that promoted seed germination and increased shoot and root
dry weight at the early-stage development.
C3  - CEESME Central and Eastern Europe Symposium on Microbial Ecology
T1  - High herbicide concentration tolerant bacteria in plant growth promotion
EP  - 98
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2163
ER  - 

@conference{
author = "Galić, Ivana and Bertani, Iris and Bez, Cristina and Stanković, Nada and Venturi, Vittorio",
year = "2023",
abstract = "organisms, including microorganisms. In the global effort to increase agriculture sustainability, there
is an emerging need for herbicide-resilient plant growth-promoting (PGP) bacteria that improve
essential crops yield. From S-metolachlor-contaminated soils, we isolated 122 strains of high herbicide
concentration tolerant (HHCT) bacteria capable of growing on or using herbicide as their sole carbon
source. The aim of our study was to identify HHCT bacteria with the highest PGP potential, soil quality
improvement ability, biocontrol activity, and plant colonization capability; then combine them into
consortia that include all these traits, and test them in planta. PGP property evaluation included assays
for nitrogen fixation, phosphate solubilization, IAA, siderophore, and exopolysaccharide production,
detection of extracellular enzymatic activities, and motility. Strains were identified through 16S rDNA
sequencing. Biocontrol potential was evaluated against plant pathogens such as Fusarium sp. , and P.
fuscovaginae. The most promising candidates were combined in three consortia for growth-chamber
experiments to investigate the promotion of maize seed germination, early-stage plant development,
and root colonization evaluated by metagenomic analysis. The HHCT bacteria showed promising
PGP potential. 32% of the collection exhibited 3-4 of four tested direct PGP mechanisms. 22 isolates
had antifungal, and 13 had antibacterial activity. The strains with the highest in vitro PGP activity
predominantly belonged to Serratia, Pseudomonas, and Sphingobacterium genera. These strains
formed the most effective consortium that promoted seed germination and increased shoot and root
dry weight at the early-stage development.",
journal = "CEESME Central and Eastern Europe Symposium on Microbial Ecology",
title = "High herbicide concentration tolerant bacteria in plant growth promotion",
pages = "98-98",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2163"
}

Galić, I., Bertani, I., Bez, C., Stanković, N.,& Venturi, V.. (2023). High herbicide concentration tolerant bacteria in plant growth promotion. in CEESME Central and Eastern Europe Symposium on Microbial Ecology, 98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2163

Galić I, Bertani I, Bez C, Stanković N, Venturi V. High herbicide concentration tolerant bacteria in plant growth promotion. in CEESME Central and Eastern Europe Symposium on Microbial Ecology. 2023;:98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2163 .

Galić, Ivana, Bertani, Iris, Bez, Cristina, Stanković, Nada, Venturi, Vittorio, "High herbicide concentration tolerant bacteria in plant growth promotion" in CEESME Central and Eastern Europe Symposium on Microbial Ecology (2023):98-98,
https://hdl.handle.net/21.15107/rcub_imagine_2163 .

TY  - JOUR
AU  - Đokić, Lidija
AU  - Stanković, Nada
AU  - Galić, Ivana
AU  - Morić, Ivana
AU  - Radaković, Nataša
AU  - Segan, Sandra
AU  - Pavić, Aleksandar
AU  - Šenerović, Lidija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1596
AB  - Bacterial infections have become increasingly difficult to treat due to the occurrence of antibiotic-resistant strains. A promising strategy to increase the efficacy of therapy is to combine antibacterials with agents that decrease pathogen virulence via the modulation of the quorum sensing (QS). Lactonases inhibit acylated homoserine lactone (AHL)-mediated QS in Gram-negative bacteria, including the leading nosocomial pathogen Pseudomonas aeruginosa. Here we describe the characteristics of heterologously expressed YtnP lactonase from Bacillus paralicheniformis ZP1 (YtnP-ZP1) isolated from agricultural soil using the culture enrichment method. Purified YtnP-ZP1 hydrolyzed different AHLs with preference to substrates with long acyl residues as evaluated in assays with biosensors and HPLC. The enzyme showed good thermostability and activity in a wide temperature range. YtnP-ZP1 in 50 mu g mL(-1) concentration reduced the amount of P. aeruginosa-produced long-chain AHLs by 85%, while it hydrolyzed 50% of short-chain AHLs. Incubation of P. aeruginosa PAO1 with YtnP-ZP1 reduced its swarming motility and elastolytic activity without bactericidal effect. YtnP-ZP1 caused the inhibition of biofilm formation and disintegration of mature biofilms in P. aeruginosa PAO1 and multiresistant clinical strain BR5H that was visualized by crystal violet staining. The treatment with YtnP-ZP1 in concentrations higher than 25 mu g mL(-1) improved the survival of P. aeruginosa PAO1-infected zebrafish (Danio rerio), rescuing 80% of embryos, while in combination with tobramycin or gentamicin survival rate increased to 100%. The treatment of P. aeruginosa PAO1 biofilms on infected zebrafish tail wounds with 50 mu g mL(-1) YtnP-ZP1 and 2 x MIC tobramycin led to infection clearing in 2 days. The extensive toxicity studies proved YtnP-ZP1 was non-toxic to human cells and zebrafish. In conclusion, novel YtnP-ZP1 lactonase with its effective anti-virulence activity could be used to increase the efficacy of clinically approved antibiotics in clearing both systemic and biofilm-associated P. aeruginosa infections.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo
VL  - 13
DO  - 10.3389/fmicb.2022.906312
ER  - 

@article{
author = "Đokić, Lidija and Stanković, Nada and Galić, Ivana and Morić, Ivana and Radaković, Nataša and Segan, Sandra and Pavić, Aleksandar and Šenerović, Lidija",
year = "2022",
abstract = "Bacterial infections have become increasingly difficult to treat due to the occurrence of antibiotic-resistant strains. A promising strategy to increase the efficacy of therapy is to combine antibacterials with agents that decrease pathogen virulence via the modulation of the quorum sensing (QS). Lactonases inhibit acylated homoserine lactone (AHL)-mediated QS in Gram-negative bacteria, including the leading nosocomial pathogen Pseudomonas aeruginosa. Here we describe the characteristics of heterologously expressed YtnP lactonase from Bacillus paralicheniformis ZP1 (YtnP-ZP1) isolated from agricultural soil using the culture enrichment method. Purified YtnP-ZP1 hydrolyzed different AHLs with preference to substrates with long acyl residues as evaluated in assays with biosensors and HPLC. The enzyme showed good thermostability and activity in a wide temperature range. YtnP-ZP1 in 50 mu g mL(-1) concentration reduced the amount of P. aeruginosa-produced long-chain AHLs by 85%, while it hydrolyzed 50% of short-chain AHLs. Incubation of P. aeruginosa PAO1 with YtnP-ZP1 reduced its swarming motility and elastolytic activity without bactericidal effect. YtnP-ZP1 caused the inhibition of biofilm formation and disintegration of mature biofilms in P. aeruginosa PAO1 and multiresistant clinical strain BR5H that was visualized by crystal violet staining. The treatment with YtnP-ZP1 in concentrations higher than 25 mu g mL(-1) improved the survival of P. aeruginosa PAO1-infected zebrafish (Danio rerio), rescuing 80% of embryos, while in combination with tobramycin or gentamicin survival rate increased to 100%. The treatment of P. aeruginosa PAO1 biofilms on infected zebrafish tail wounds with 50 mu g mL(-1) YtnP-ZP1 and 2 x MIC tobramycin led to infection clearing in 2 days. The extensive toxicity studies proved YtnP-ZP1 was non-toxic to human cells and zebrafish. In conclusion, novel YtnP-ZP1 lactonase with its effective anti-virulence activity could be used to increase the efficacy of clinically approved antibiotics in clearing both systemic and biofilm-associated P. aeruginosa infections.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo",
volume = "13",
doi = "10.3389/fmicb.2022.906312"
}

Đokić, L., Stanković, N., Galić, I., Morić, I., Radaković, N., Segan, S., Pavić, A.,& Šenerović, L.. (2022). Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 13.
https://doi.org/10.3389/fmicb.2022.906312

Đokić L, Stanković N, Galić I, Morić I, Radaković N, Segan S, Pavić A, Šenerović L. Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo. in Frontiers in Microbiology. 2022;13.
doi:10.3389/fmicb.2022.906312 .

Đokić, Lidija, Stanković, Nada, Galić, Ivana, Morić, Ivana, Radaković, Nataša, Segan, Sandra, Pavić, Aleksandar, Šenerović, Lidija, "Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo" in Frontiers in Microbiology, 13 (2022),
https://doi.org/10.3389/fmicb.2022.906312 . .

TY  - CONF
AU  - Galić, Ivana
AU  - Šenerović, Lidija
AU  - Stanković, Nada
PY  - 2022
UR  - https://2022.eccmid.org/abstracts/posters
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2162
AB  - Soils contain microorganisms capable of causing diseases in humans, either
as opportunistic or as obligate pathogens. The application of herbicides
increases the prevalence of antibiotic resistance genes and mobile genetic
elements, which expands the competitive niche of pathogenic microbes. This
implies that herbicides could impose selective pressure driving the spread of
antibiotic resistance and therefore making heavily herbicide treated agricultural
soil a possible reservoir of multiresistant opportunistic human pathogens.
From the soil exposed to chlorinated aromatic herbicides S-metolachlor
and isoxaflutole, semi-persistent selective herbicides widely used in
European Union and Serbia we isolated bacteria able to grow on herbicide as
the sole carbon source. Bacterial isolates were assessed for various virulence
traits and resistance to antibiotics in order to identify the strains with high
pathogenic potential.
C3  - European Congress of Clinical Microbiology & Infectious Diseases
T1  - Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans
VL  - 32
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2162
ER  - 

@conference{
author = "Galić, Ivana and Šenerović, Lidija and Stanković, Nada",
year = "2022",
abstract = "Soils contain microorganisms capable of causing diseases in humans, either
as opportunistic or as obligate pathogens. The application of herbicides
increases the prevalence of antibiotic resistance genes and mobile genetic
elements, which expands the competitive niche of pathogenic microbes. This
implies that herbicides could impose selective pressure driving the spread of
antibiotic resistance and therefore making heavily herbicide treated agricultural
soil a possible reservoir of multiresistant opportunistic human pathogens.
From the soil exposed to chlorinated aromatic herbicides S-metolachlor
and isoxaflutole, semi-persistent selective herbicides widely used in
European Union and Serbia we isolated bacteria able to grow on herbicide as
the sole carbon source. Bacterial isolates were assessed for various virulence
traits and resistance to antibiotics in order to identify the strains with high
pathogenic potential.",
journal = "European Congress of Clinical Microbiology & Infectious Diseases",
title = "Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans",
volume = "32",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2162"
}

Galić, I., Šenerović, L.,& Stanković, N.. (2022). Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans. in European Congress of Clinical Microbiology & Infectious Diseases, 32.
https://hdl.handle.net/21.15107/rcub_imagine_2162

Galić I, Šenerović L, Stanković N. Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans. in European Congress of Clinical Microbiology & Infectious Diseases. 2022;32.
https://hdl.handle.net/21.15107/rcub_imagine_2162 .

Galić, Ivana, Šenerović, Lidija, Stanković, Nada, "Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans" in European Congress of Clinical Microbiology & Infectious Diseases, 32 (2022),
https://hdl.handle.net/21.15107/rcub_imagine_2162 .

TY  - JOUR
AU  - Radaković, Nataša
AU  - Nikolić, Andrea
AU  - Terzić-Jovanović, Nataša
AU  - Stojković, Pavle
AU  - Stanković, Nada
AU  - Solaja, Bogdan
AU  - Opsenica, Igor
AU  - Pavić, Aleksandar
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1593
AB  - Candida albicans remains the main causal agent of candidiasis, the most common fungal infection with disturbingly high mortality rates worldwide. The limited diversity and efficacy of clinical antifungal drugs, exacerbated by emerging drug resistance, have resulted in the failure of current antifungal therapies. This imposes an urgent demand for the development of innovative strategies for effective eradication of candidal infections. While the existing clinical drugs display fungicidal or fungistatic activity, the strategy specifically targeting C. albicans filamentation, as the most important virulence trait, represents an attractive approach for overcoming the drawbacks related to clinical antifungals. The results acquired in this study revealed the significant potential of 5-aminotetrazoles as a new class of effective and safe anti-virulence agents. Moreover, these novel agents were active when applied both alone and in combination with clinically approved polyenes. Complete prevention of C. albicans morphogenetic yeast-to-hyphae transition was achieved at doses as low as 1.3 mM under conditions mimicking various filamentation-responsive stimuli in the human body, while no cardio-or hepatotoxicity was observed at doses as high as 200 mM. The treatment of C. albicans-infected zebrafish embryos with nystatin alone had low efficacy, while the combination of nystatin and selected 5aminotetrazoles prevented fungal filamentation, successfully eliminating the infection and rescuing the infected embryos from lethal disseminated candidiasis. In addition, the most potent anti-virulence 5aminotetrazole prevented C. albicans in developing the resistance to nystatin when applied in combination, keeping the fungus sensitive to the antifungal drug.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis
VL  - 230
DO  - 10.1016/j.ejmech.2022.114137
ER  - 

@article{
author = "Radaković, Nataša and Nikolić, Andrea and Terzić-Jovanović, Nataša and Stojković, Pavle and Stanković, Nada and Solaja, Bogdan and Opsenica, Igor and Pavić, Aleksandar",
year = "2022",
abstract = "Candida albicans remains the main causal agent of candidiasis, the most common fungal infection with disturbingly high mortality rates worldwide. The limited diversity and efficacy of clinical antifungal drugs, exacerbated by emerging drug resistance, have resulted in the failure of current antifungal therapies. This imposes an urgent demand for the development of innovative strategies for effective eradication of candidal infections. While the existing clinical drugs display fungicidal or fungistatic activity, the strategy specifically targeting C. albicans filamentation, as the most important virulence trait, represents an attractive approach for overcoming the drawbacks related to clinical antifungals. The results acquired in this study revealed the significant potential of 5-aminotetrazoles as a new class of effective and safe anti-virulence agents. Moreover, these novel agents were active when applied both alone and in combination with clinically approved polyenes. Complete prevention of C. albicans morphogenetic yeast-to-hyphae transition was achieved at doses as low as 1.3 mM under conditions mimicking various filamentation-responsive stimuli in the human body, while no cardio-or hepatotoxicity was observed at doses as high as 200 mM. The treatment of C. albicans-infected zebrafish embryos with nystatin alone had low efficacy, while the combination of nystatin and selected 5aminotetrazoles prevented fungal filamentation, successfully eliminating the infection and rescuing the infected embryos from lethal disseminated candidiasis. In addition, the most potent anti-virulence 5aminotetrazole prevented C. albicans in developing the resistance to nystatin when applied in combination, keeping the fungus sensitive to the antifungal drug.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis",
volume = "230",
doi = "10.1016/j.ejmech.2022.114137"
}

Radaković, N., Nikolić, A., Terzić-Jovanović, N., Stojković, P., Stanković, N., Solaja, B., Opsenica, I.,& Pavić, A.. (2022). Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 230.
https://doi.org/10.1016/j.ejmech.2022.114137

Radaković N, Nikolić A, Terzić-Jovanović N, Stojković P, Stanković N, Solaja B, Opsenica I, Pavić A. Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis. in European Journal of Medicinal Chemistry. 2022;230.
doi:10.1016/j.ejmech.2022.114137 .

Radaković, Nataša, Nikolić, Andrea, Terzić-Jovanović, Nataša, Stojković, Pavle, Stanković, Nada, Solaja, Bogdan, Opsenica, Igor, Pavić, Aleksandar, "Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis" in European Journal of Medicinal Chemistry, 230 (2022),
https://doi.org/10.1016/j.ejmech.2022.114137 . .

TY  - JOUR
AU  - Schneider, Olha
AU  - Ilić-Tomić, Tatjana
AU  - Rueckert, Christian
AU  - Kalinowski, Joern
AU  - Gencić, Marija S.
AU  - Zivković, Milena Z.
AU  - Stanković, Nada
AU  - Radulović, Niko S.
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Zotchev, Sergey B.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1107
AB  - Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved. Analysis of the Streptomyces sp. NP10 draft genome revealed that it is closely related to several strains of Streptomyces griseus. Comparative analyses of secondary metabolite biosynthetic gene clusters, as well as those presumably involved in FA biosynthesis, allowed identification of an unusual cluster C12-2, which could be identified in only one other S. griseus-related streptomycete. To prove the involvement of identified cluster in FFA biosynthesis, one of its three ketosynthase genes was insertionally inactivated to generate mutant strain mNP10. Accumulation of FFAs in mNP10 was almost completely abolished, reaching less than 0.01% compared to the wild-type strain. Cloning and transfer of the C12-2 cluster to the mNP10 mutant partially restored FFA production, albeit to a low level. The discovery of this rare FFA biosynthesis cluster opens possibilities for detailed characterization of the roles of individual genes and their products in the biosynthesis of FFAs in NP10.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10
VL  - 9
DO  - 10.3389/fmicb.2018.01302
ER  - 

@article{
author = "Schneider, Olha and Ilić-Tomić, Tatjana and Rueckert, Christian and Kalinowski, Joern and Gencić, Marija S. and Zivković, Milena Z. and Stanković, Nada and Radulović, Niko S. and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Zotchev, Sergey B.",
year = "2018",
abstract = "Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved. Analysis of the Streptomyces sp. NP10 draft genome revealed that it is closely related to several strains of Streptomyces griseus. Comparative analyses of secondary metabolite biosynthetic gene clusters, as well as those presumably involved in FA biosynthesis, allowed identification of an unusual cluster C12-2, which could be identified in only one other S. griseus-related streptomycete. To prove the involvement of identified cluster in FFA biosynthesis, one of its three ketosynthase genes was insertionally inactivated to generate mutant strain mNP10. Accumulation of FFAs in mNP10 was almost completely abolished, reaching less than 0.01% compared to the wild-type strain. Cloning and transfer of the C12-2 cluster to the mNP10 mutant partially restored FFA production, albeit to a low level. The discovery of this rare FFA biosynthesis cluster opens possibilities for detailed characterization of the roles of individual genes and their products in the biosynthesis of FFAs in NP10.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10",
volume = "9",
doi = "10.3389/fmicb.2018.01302"
}

Schneider, O., Ilić-Tomić, T., Rueckert, C., Kalinowski, J., Gencić, M. S., Zivković, M. Z., Stanković, N., Radulović, N. S., Vasiljević, B., Nikodinović-Runić, J.,& Zotchev, S. B.. (2018). Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.01302

Schneider O, Ilić-Tomić T, Rueckert C, Kalinowski J, Gencić MS, Zivković MZ, Stanković N, Radulović NS, Vasiljević B, Nikodinović-Runić J, Zotchev SB. Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.01302 .

Schneider, Olha, Ilić-Tomić, Tatjana, Rueckert, Christian, Kalinowski, Joern, Gencić, Marija S., Zivković, Milena Z., Stanković, Nada, Radulović, Niko S., Vasiljević, Branka, Nikodinović-Runić, Jasmina, Zotchev, Sergey B., "Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.01302 . .

TY  - JOUR
AU  - Lippert, Rainer
AU  - Shubina, Tatyana E.
AU  - Vojnović, Sandra
AU  - Pavić, Aleksandar
AU  - Veselinović, Jovana
AU  - Nikodinović-Runić, Jasmina
AU  - Stanković, Nada
AU  - Ivanović-Burmazović, Ivana
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1078
AB  - In order to improve antimicrobial effects of previously studied meso-tetrakis(4-ferrocenylphenyl)porphyrin 1, we have modified its structure by replacing two trans-positioned ferrocenylphenyl moieties with methoxy methylene substituted tert-butylphenyl moieties. Newly synthesized 5(4),15(4)-bis-(ferrocenyl)-10(4),20(4)-bis-(tert-butyl)10(2),10(6),20(2),20(6)-tetrakis-(methoxy-methylene)-5,10,15,20-tetraphenylporphyrin 4 was chemically characterized in detail (by NMR, UV/Vis, IR, MALDI-TOF and ESI MS spectrometry, cyclic voltammetry, prediction of the relative lipophilicity as well as computational methods) and its biological effects were studied in terms of its antibacterial and antifungal activity (both with and without photoactivation), cytotoxicity, hemolysis and DNA cleavage. New ferrocene bearing porphyrin 4 has demonstrated a broader antimicrobial spectrum and modified effects on eukaryotic cells compared to 1. This was discussed in terms of its i) increased lipophilicity, while exhibiting.lower toxicity, and ii) the redox potential of a two-electron process that is shifted to lower values, in comparison to ferrocene, thus, entering the physiologically available range and being activated towards redox interactions with biomolecules.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - Redox behavior and biological properties of ferrocene bearing porphyrins
EP  - 89
SP  - 76
VL  - 171
DO  - 10.1016/j.jinorgbio.2017.03.002
ER  - 

@article{
author = "Lippert, Rainer and Shubina, Tatyana E. and Vojnović, Sandra and Pavić, Aleksandar and Veselinović, Jovana and Nikodinović-Runić, Jasmina and Stanković, Nada and Ivanović-Burmazović, Ivana",
year = "2017",
abstract = "In order to improve antimicrobial effects of previously studied meso-tetrakis(4-ferrocenylphenyl)porphyrin 1, we have modified its structure by replacing two trans-positioned ferrocenylphenyl moieties with methoxy methylene substituted tert-butylphenyl moieties. Newly synthesized 5(4),15(4)-bis-(ferrocenyl)-10(4),20(4)-bis-(tert-butyl)10(2),10(6),20(2),20(6)-tetrakis-(methoxy-methylene)-5,10,15,20-tetraphenylporphyrin 4 was chemically characterized in detail (by NMR, UV/Vis, IR, MALDI-TOF and ESI MS spectrometry, cyclic voltammetry, prediction of the relative lipophilicity as well as computational methods) and its biological effects were studied in terms of its antibacterial and antifungal activity (both with and without photoactivation), cytotoxicity, hemolysis and DNA cleavage. New ferrocene bearing porphyrin 4 has demonstrated a broader antimicrobial spectrum and modified effects on eukaryotic cells compared to 1. This was discussed in terms of its i) increased lipophilicity, while exhibiting.lower toxicity, and ii) the redox potential of a two-electron process that is shifted to lower values, in comparison to ferrocene, thus, entering the physiologically available range and being activated towards redox interactions with biomolecules.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "Redox behavior and biological properties of ferrocene bearing porphyrins",
pages = "89-76",
volume = "171",
doi = "10.1016/j.jinorgbio.2017.03.002"
}

Lippert, R., Shubina, T. E., Vojnović, S., Pavić, A., Veselinović, J., Nikodinović-Runić, J., Stanković, N.,& Ivanović-Burmazović, I.. (2017). Redox behavior and biological properties of ferrocene bearing porphyrins. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 171, 76-89.
https://doi.org/10.1016/j.jinorgbio.2017.03.002

Lippert R, Shubina TE, Vojnović S, Pavić A, Veselinović J, Nikodinović-Runić J, Stanković N, Ivanović-Burmazović I. Redox behavior and biological properties of ferrocene bearing porphyrins. in Journal of Inorganic Biochemistry. 2017;171:76-89.
doi:10.1016/j.jinorgbio.2017.03.002 .

Lippert, Rainer, Shubina, Tatyana E., Vojnović, Sandra, Pavić, Aleksandar, Veselinović, Jovana, Nikodinović-Runić, Jasmina, Stanković, Nada, Ivanović-Burmazović, Ivana, "Redox behavior and biological properties of ferrocene bearing porphyrins" in Journal of Inorganic Biochemistry, 171 (2017):76-89,
https://doi.org/10.1016/j.jinorgbio.2017.03.002 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Zivković, S.
AU  - Pekmezović, M.
AU  - Stanković, Nada
AU  - Vojnović, Sandra
AU  - Vasiljević, Branka
AU  - Šenerović, Lidija
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/876
AB  - AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin
EP  - 1434
IS  - 6
SP  - 1426
VL  - 118
DO  - 10.1111/jam.12811
ER  - 

@article{
author = "Milisavljević, Mira and Zivković, S. and Pekmezović, M. and Stanković, Nada and Vojnović, Sandra and Vasiljević, Branka and Šenerović, Lidija",
year = "2015",
abstract = "AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin",
pages = "1434-1426",
number = "6",
volume = "118",
doi = "10.1111/jam.12811"
}

Milisavljević, M., Zivković, S., Pekmezović, M., Stanković, N., Vojnović, S., Vasiljević, B.,& Šenerović, L.. (2015). Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology
Wiley, Hoboken., 118(6), 1426-1434.
https://doi.org/10.1111/jam.12811

Milisavljević M, Zivković S, Pekmezović M, Stanković N, Vojnović S, Vasiljević B, Šenerović L. Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology. 2015;118(6):1426-1434.
doi:10.1111/jam.12811 .

Milisavljević, Mira, Zivković, S., Pekmezović, M., Stanković, Nada, Vojnović, Sandra, Vasiljević, Branka, Šenerović, Lidija, "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin" in Journal of Applied Microbiology, 118, no. 6 (2015):1426-1434,
https://doi.org/10.1111/jam.12811 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Zivković, S.
AU  - Pekmezović, M.
AU  - Stanković, Nada
AU  - Vojnović, Sandra
AU  - Vasiljević, Branka
AU  - Šenerović, Lidija
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1608
AB  - AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin
EP  - 1434
IS  - 6
SP  - 1426
VL  - 118
DO  - 10.1111/jam.12811
ER  - 

@article{
author = "Milisavljević, Mira and Zivković, S. and Pekmezović, M. and Stanković, Nada and Vojnović, Sandra and Vasiljević, Branka and Šenerović, Lidija",
year = "2015",
abstract = "AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin",
pages = "1434-1426",
number = "6",
volume = "118",
doi = "10.1111/jam.12811"
}

Milisavljević, M., Zivković, S., Pekmezović, M., Stanković, N., Vojnović, S., Vasiljević, B.,& Šenerović, L.. (2015). Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology
Wiley, Hoboken., 118(6), 1426-1434.
https://doi.org/10.1111/jam.12811

Milisavljević M, Zivković S, Pekmezović M, Stanković N, Vojnović S, Vasiljević B, Šenerović L. Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology. 2015;118(6):1426-1434.
doi:10.1111/jam.12811 .

Milisavljević, Mira, Zivković, S., Pekmezović, M., Stanković, Nada, Vojnović, Sandra, Vasiljević, Branka, Šenerović, Lidija, "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin" in Journal of Applied Microbiology, 118, no. 6 (2015):1426-1434,
https://doi.org/10.1111/jam.12811 . .

TY  - JOUR
AU  - Stanković, Nada
AU  - Šenerović, Lidija
AU  - Ilić-Tomić, Tatjana
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/776
AB  - The growing demand to fulfill the needs of present-day medicine in terms of novel effective molecules has lead to reexamining some of the old and known bacterial secondary metabolites. Bacterial prodigiosins (prodiginines) have a long history of being re markable multipurpose compounds, best examined for their anticancer and antimalarial activities. Production of prodigiosin in the most common producer strain Serratia marcescens has been described in great detail. However, few reports have discussed the ecophysiological roles of these molecules in the producing strains, as well as their antibiotic and UV-protective properties. This review describes recent advances in the production process, biosynthesis, properties, and applications of bacterial prodigiosins. Special emphasis is put on undecylprodigiosin which has generally been a less studied member of the prodigiosin family. In addition, it has been suggested that proteins involved in undecylprodigiosin synthesis, RedG and RedH, could be a useful addition to the biocatalytic toolbox being able to mediate regio- and stereoselective oxidative cyclization. Judging by the number of recent references (216 for the 2007-2013 period), it has become clear that undecylprodigiosin and other bacterial prodigiosins still hold surprises in terms of valuable properties and applicative potential to medical and other industrial fields and that they still deserve continuing research curiosity.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Properties and applications of undecylprodigiosin and other bacterial prodigiosins
EP  - 3858
IS  - 9
SP  - 3841
VL  - 98
DO  - 10.1007/s00253-014-5590-1
ER  - 

@article{
author = "Stanković, Nada and Šenerović, Lidija and Ilić-Tomić, Tatjana and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2014",
abstract = "The growing demand to fulfill the needs of present-day medicine in terms of novel effective molecules has lead to reexamining some of the old and known bacterial secondary metabolites. Bacterial prodigiosins (prodiginines) have a long history of being re markable multipurpose compounds, best examined for their anticancer and antimalarial activities. Production of prodigiosin in the most common producer strain Serratia marcescens has been described in great detail. However, few reports have discussed the ecophysiological roles of these molecules in the producing strains, as well as their antibiotic and UV-protective properties. This review describes recent advances in the production process, biosynthesis, properties, and applications of bacterial prodigiosins. Special emphasis is put on undecylprodigiosin which has generally been a less studied member of the prodigiosin family. In addition, it has been suggested that proteins involved in undecylprodigiosin synthesis, RedG and RedH, could be a useful addition to the biocatalytic toolbox being able to mediate regio- and stereoselective oxidative cyclization. Judging by the number of recent references (216 for the 2007-2013 period), it has become clear that undecylprodigiosin and other bacterial prodigiosins still hold surprises in terms of valuable properties and applicative potential to medical and other industrial fields and that they still deserve continuing research curiosity.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Properties and applications of undecylprodigiosin and other bacterial prodigiosins",
pages = "3858-3841",
number = "9",
volume = "98",
doi = "10.1007/s00253-014-5590-1"
}

Stanković, N., Šenerović, L., Ilić-Tomić, T., Vasiljević, B.,& Nikodinović-Runić, J.. (2014). Properties and applications of undecylprodigiosin and other bacterial prodigiosins. in Applied Microbiology and Biotechnology
Springer, New York., 98(9), 3841-3858.
https://doi.org/10.1007/s00253-014-5590-1

Stanković N, Šenerović L, Ilić-Tomić T, Vasiljević B, Nikodinović-Runić J. Properties and applications of undecylprodigiosin and other bacterial prodigiosins. in Applied Microbiology and Biotechnology. 2014;98(9):3841-3858.
doi:10.1007/s00253-014-5590-1 .

Stanković, Nada, Šenerović, Lidija, Ilić-Tomić, Tatjana, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Properties and applications of undecylprodigiosin and other bacterial prodigiosins" in Applied Microbiology and Biotechnology, 98, no. 9 (2014):3841-3858,
https://doi.org/10.1007/s00253-014-5590-1 . .

TY  - JOUR
AU  - Lippert, Rainer
AU  - Vojnović, Sandra
AU  - Mitrović, Aleksandra
AU  - Jux, Norbert
AU  - Ivanović-Burmazović, Ivana
AU  - Vasiljević, Branka
AU  - Stanković, Nada
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/705
AB  - Ferrocene-substituted porphyrin RL-91 exhibits antifungal activity against opportune human pathogen Candida albicans. RL-91 efficiently inhibits growth of both planktonic C albicans cells and cells within biofilms without photoactivation. The minimal inhibitory concentration for plankton form (PMIC) was established to be 100 mu g/mL and the same concentration killed 80% of sessile cells in the mature biofilm (SMIC80). Furthermore PMIC of RL-91 efficiently prevents C albicans biofilm formation. RL-91 is cytotoxic for human fibroblasts in vitro in concentration of 10 mu g/mL, however it does not cause hemolysis in concentrations of up to 50 mu g/mL. These findings open possibility for application of RL-91 as an antifungal agent for external antibiofilm treatment of medical devices as well as a scaffold for further development of porphyrin based systemic antifungals.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Bioorganic & Medicinal Chemistry Letters
T1  - Effect of ferrocene-substituted porphyrin RL-91 on Candida albicans biofilm formation
EP  - 3511
IS  - 15
SP  - 3506
VL  - 24
DO  - 10.1016/j.bmcl.2014.05.061
ER  - 

@article{
author = "Lippert, Rainer and Vojnović, Sandra and Mitrović, Aleksandra and Jux, Norbert and Ivanović-Burmazović, Ivana and Vasiljević, Branka and Stanković, Nada",
year = "2014",
abstract = "Ferrocene-substituted porphyrin RL-91 exhibits antifungal activity against opportune human pathogen Candida albicans. RL-91 efficiently inhibits growth of both planktonic C albicans cells and cells within biofilms without photoactivation. The minimal inhibitory concentration for plankton form (PMIC) was established to be 100 mu g/mL and the same concentration killed 80% of sessile cells in the mature biofilm (SMIC80). Furthermore PMIC of RL-91 efficiently prevents C albicans biofilm formation. RL-91 is cytotoxic for human fibroblasts in vitro in concentration of 10 mu g/mL, however it does not cause hemolysis in concentrations of up to 50 mu g/mL. These findings open possibility for application of RL-91 as an antifungal agent for external antibiofilm treatment of medical devices as well as a scaffold for further development of porphyrin based systemic antifungals.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Bioorganic & Medicinal Chemistry Letters",
title = "Effect of ferrocene-substituted porphyrin RL-91 on Candida albicans biofilm formation",
pages = "3511-3506",
number = "15",
volume = "24",
doi = "10.1016/j.bmcl.2014.05.061"
}

Lippert, R., Vojnović, S., Mitrović, A., Jux, N., Ivanović-Burmazović, I., Vasiljević, B.,& Stanković, N.. (2014). Effect of ferrocene-substituted porphyrin RL-91 on Candida albicans biofilm formation. in Bioorganic & Medicinal Chemistry Letters
Pergamon-Elsevier Science Ltd, Oxford., 24(15), 3506-3511.
https://doi.org/10.1016/j.bmcl.2014.05.061

Lippert R, Vojnović S, Mitrović A, Jux N, Ivanović-Burmazović I, Vasiljević B, Stanković N. Effect of ferrocene-substituted porphyrin RL-91 on Candida albicans biofilm formation. in Bioorganic & Medicinal Chemistry Letters. 2014;24(15):3506-3511.
doi:10.1016/j.bmcl.2014.05.061 .

Lippert, Rainer, Vojnović, Sandra, Mitrović, Aleksandra, Jux, Norbert, Ivanović-Burmazović, Ivana, Vasiljević, Branka, Stanković, Nada, "Effect of ferrocene-substituted porphyrin RL-91 on Candida albicans biofilm formation" in Bioorganic & Medicinal Chemistry Letters, 24, no. 15 (2014):3506-3511,
https://doi.org/10.1016/j.bmcl.2014.05.061 . .

TY  - JOUR
AU  - Stanković, Nada
AU  - Šenerović, Lidija
AU  - Bojić-Trbojević, Z.
AU  - Vucković, I.
AU  - Vicovac, L.
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/674
AB  - AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity
EP  - 1306
IS  - 6
SP  - 1297
VL  - 115
DO  - 10.1111/jam.12326
ER  - 

@article{
author = "Stanković, Nada and Šenerović, Lidija and Bojić-Trbojević, Z. and Vucković, I. and Vicovac, L. and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2013",
abstract = "AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity",
pages = "1306-1297",
number = "6",
volume = "115",
doi = "10.1111/jam.12326"
}

Stanković, N., Šenerović, L., Bojić-Trbojević, Z., Vucković, I., Vicovac, L., Vasiljević, B.,& Nikodinović-Runić, J.. (2013). Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology
Wiley, Hoboken., 115(6), 1297-1306.
https://doi.org/10.1111/jam.12326

Stanković N, Šenerović L, Bojić-Trbojević Z, Vucković I, Vicovac L, Vasiljević B, Nikodinović-Runić J. Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology. 2013;115(6):1297-1306.
doi:10.1111/jam.12326 .

Stanković, Nada, Šenerović, Lidija, Bojić-Trbojević, Z., Vucković, I., Vicovac, L., Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity" in Journal of Applied Microbiology, 115, no. 6 (2013):1297-1306,
https://doi.org/10.1111/jam.12326 . .

TY  - THES
AU  - Stanković, Nada
PY  - 2012
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=87
UR  - https://nardus.mpn.gov.rs/handle/123456789/2068
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:3492/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024553906
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/26
AB  - Actinomycetes are Gram-positive soil bacteria known as producers of secondary bioactive metabolites. In the search for new bioactive compounds collection of actinomycetes from the Laboratory for Microbial Molecular Genetics and Ecology (Institute of Molecular Genetic and Genetic Engineering, University of Belgrade) was screened according to several criteria - bioactivity, the presence of the genes for polyketide synthase (PKS), pigmentation, and crude culture extracts absorption profile in ultraviolet and visible (UV/Vis) spectra. Based on these we have isolated three strains NP10, JS520, and Streptomyces durmitotensis, previously renewed for didehydroroflamicoin (DDHR) production. Isolates NP10 and JS520 were identified to belong to the genus Streptomyces. Streptomyces sp. NP10 accumulated branched chain fatty acids, predominately isopalmitic, and cyclic dipeptides consisting of proline in combination with valine, isoleucine or alanine, that have a stimulating effect on the trophoblast cell line in culture at low concentrations (1 ng/ml and 1 mg/ml) and cytotoxic effect at high concentrations (1 mg/ml). Streptomyces sp. JS520 was determined to be exceptionally good producer of pigment undecylprodigiosine (UP) with antibacterial, antifungal, antioxidant and UV protective activities showing cytotoxic effects on trophoblast cell line in culture at high concentrations (1 ng/ml). Cytotoxicity of polyene macrolide antibiotic DDHR from S. durmitotensis was confirmed and it’s antifungal effects were shown. PKS cluster responsible for DDHR synthesis was subcloned in the cosmid library and partially sequenced. Optimization of culture conditions resulted in 2.12 times increased production of UP, and 1.1 times increased production of DDHR.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces
T1  - Isolation and characterization of bioactive secondary metabolites from selected Streptomyces strains
UR  - https://hdl.handle.net/21.15107/rcub_nardus_2068
ER  - 

@phdthesis{
author = "Stanković, Nada",
year = "2012",
abstract = "Actinomycetes are Gram-positive soil bacteria known as producers of secondary bioactive metabolites. In the search for new bioactive compounds collection of actinomycetes from the Laboratory for Microbial Molecular Genetics and Ecology (Institute of Molecular Genetic and Genetic Engineering, University of Belgrade) was screened according to several criteria - bioactivity, the presence of the genes for polyketide synthase (PKS), pigmentation, and crude culture extracts absorption profile in ultraviolet and visible (UV/Vis) spectra. Based on these we have isolated three strains NP10, JS520, and Streptomyces durmitotensis, previously renewed for didehydroroflamicoin (DDHR) production. Isolates NP10 and JS520 were identified to belong to the genus Streptomyces. Streptomyces sp. NP10 accumulated branched chain fatty acids, predominately isopalmitic, and cyclic dipeptides consisting of proline in combination with valine, isoleucine or alanine, that have a stimulating effect on the trophoblast cell line in culture at low concentrations (1 ng/ml and 1 mg/ml) and cytotoxic effect at high concentrations (1 mg/ml). Streptomyces sp. JS520 was determined to be exceptionally good producer of pigment undecylprodigiosine (UP) with antibacterial, antifungal, antioxidant and UV protective activities showing cytotoxic effects on trophoblast cell line in culture at high concentrations (1 ng/ml). Cytotoxicity of polyene macrolide antibiotic DDHR from S. durmitotensis was confirmed and it’s antifungal effects were shown. PKS cluster responsible for DDHR synthesis was subcloned in the cosmid library and partially sequenced. Optimization of culture conditions resulted in 2.12 times increased production of UP, and 1.1 times increased production of DDHR.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces, Isolation and characterization of bioactive secondary metabolites from selected Streptomyces strains",
url = "https://hdl.handle.net/21.15107/rcub_nardus_2068"
}

Stanković, N.. (2012). Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_2068

Stanković N. Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces. 2012;.
https://hdl.handle.net/21.15107/rcub_nardus_2068 .

Stanković, Nada, "Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces" (2012),
https://hdl.handle.net/21.15107/rcub_nardus_2068 .

TY  - JOUR
AU  - Stanković, Nada
AU  - Radulović, Vanja
AU  - Petković, Milos
AU  - Vucković, Ivan
AU  - Jadranin, Milka
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/604
AB  - A Gram-positive, red-pigment-producing bacterial strain, designated JS520 was isolated from the pristine sediment from the cave on mountain Miroc in Serbia. Strain was confirmed to belong to Streptomyces genus based on phenotypic and genetic analysis. Streptomyces sp. JS520 has the ability to produce exceptionally high amounts of deep red pigment into both solid and liquid media. Liquid chromatography and mass spectroscopy of the purified pigments revealed the major component to be undecylprodigiosin (93 %) with minor component being oxidatively cyclized derivative. The pigment production was affected by medium composition, temperature, pH, and the aeration rate. By medium optimization, yields of undecylprodigiosin of 138 mg l(-1) were achieved, what is the highest level of undecylprodigiosin production reported for the members of Gram-positive Streptomyces genus. Purified pigment had antimicrobial properties against bacterial Bacillus and Micrococcus species (50 mu g ml(-1)) and against Candida albicans species (100-200 mu g ml(-1) range). The ability to affect auto-oxidation of the linoleic acid was demonstrated for the purified undecylprodigiosin, suggesting antioxidative properties of this pigment. Multiple ecophysiological roles of the pigment were revealed by comparing cultures grown under pigment-producing and pigment-nonproducing conditions. Cells grown under undecylprodigiosin-producing conditions could tolerate presence of hydrogen peroxide exhibiting three times smaller zones of inhibition at 100 mM H2O2. Undecylprodigiosin-producing cells were also less susceptible to tetracycline, kanamycin, chloramphenicol, and 8-hydroxyquinoline. While the growth of the cells not producing pigment was completely inhibited by 15 min of exposure to ultraviolet light (254 nm), cells producing undecylprodigiosin and cells supplied with purified pigment in vitro showed survival rates at 22 and 8 %, respectively.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Streptomyces sp JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties
EP  - 1231
IS  - 5
SP  - 1217
VL  - 96
DO  - 10.1007/s00253-012-4237-3
ER  - 

@article{
author = "Stanković, Nada and Radulović, Vanja and Petković, Milos and Vucković, Ivan and Jadranin, Milka and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2012",
abstract = "A Gram-positive, red-pigment-producing bacterial strain, designated JS520 was isolated from the pristine sediment from the cave on mountain Miroc in Serbia. Strain was confirmed to belong to Streptomyces genus based on phenotypic and genetic analysis. Streptomyces sp. JS520 has the ability to produce exceptionally high amounts of deep red pigment into both solid and liquid media. Liquid chromatography and mass spectroscopy of the purified pigments revealed the major component to be undecylprodigiosin (93 %) with minor component being oxidatively cyclized derivative. The pigment production was affected by medium composition, temperature, pH, and the aeration rate. By medium optimization, yields of undecylprodigiosin of 138 mg l(-1) were achieved, what is the highest level of undecylprodigiosin production reported for the members of Gram-positive Streptomyces genus. Purified pigment had antimicrobial properties against bacterial Bacillus and Micrococcus species (50 mu g ml(-1)) and against Candida albicans species (100-200 mu g ml(-1) range). The ability to affect auto-oxidation of the linoleic acid was demonstrated for the purified undecylprodigiosin, suggesting antioxidative properties of this pigment. Multiple ecophysiological roles of the pigment were revealed by comparing cultures grown under pigment-producing and pigment-nonproducing conditions. Cells grown under undecylprodigiosin-producing conditions could tolerate presence of hydrogen peroxide exhibiting three times smaller zones of inhibition at 100 mM H2O2. Undecylprodigiosin-producing cells were also less susceptible to tetracycline, kanamycin, chloramphenicol, and 8-hydroxyquinoline. While the growth of the cells not producing pigment was completely inhibited by 15 min of exposure to ultraviolet light (254 nm), cells producing undecylprodigiosin and cells supplied with purified pigment in vitro showed survival rates at 22 and 8 %, respectively.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Streptomyces sp JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties",
pages = "1231-1217",
number = "5",
volume = "96",
doi = "10.1007/s00253-012-4237-3"
}

Stanković, N., Radulović, V., Petković, M., Vucković, I., Jadranin, M., Vasiljević, B.,& Nikodinović-Runić, J.. (2012). Streptomyces sp JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties. in Applied Microbiology and Biotechnology
Springer, New York., 96(5), 1217-1231.
https://doi.org/10.1007/s00253-012-4237-3

Stanković N, Radulović V, Petković M, Vucković I, Jadranin M, Vasiljević B, Nikodinović-Runić J. Streptomyces sp JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties. in Applied Microbiology and Biotechnology. 2012;96(5):1217-1231.
doi:10.1007/s00253-012-4237-3 .

Stanković, Nada, Radulović, Vanja, Petković, Milos, Vucković, Ivan, Jadranin, Milka, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Streptomyces sp JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties" in Applied Microbiology and Biotechnology, 96, no. 5 (2012):1217-1231,
https://doi.org/10.1007/s00253-012-4237-3 . .

TY  - JOUR
AU  - Šenerović, Lidija
AU  - Stanković, Nada
AU  - Ljubijankić, G.
AU  - Vasiljević, Branka
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/381
AB  - Penicilin G acilaza (PAC) je jedan od najšire korišćenih enzima u industrijskoj sintezi polusintetskih antibiotika. U ovom radu dobijeni nivo ekspresije PAC gena iz Providencia rettgeri u ekspresionom sistemu Pichia pastoris iznosio je 2.7 U/ml. Rekombinantni enzim je prečišćen i određen je njegov glikozilacioni status. Nađeno je da osim što su obe subjedinice enzima (α i β) N-glikozilovane, β subjedinica sadrži još i O-glikane. Takođe je ustanovljeno da je rekombinantna PACP. rett. stabilna u širokom pH opsegu što ju je, zajedno sa predhodno ustanovljenom visokom termostabilnošću, učinilo izuzetno privlačnim biokatalizatorom sa industrijske tačke gledišta.
AB  - Penicillin G acylase (PAC) is one of the most widely used enzymes in industrial synthesis of semi-synthetic antibiotics. The Providencia rettgeri pac gene was expressed to a level of 2.7 U/ml using the Pichia pastoris expression system. The recombinant enzyme was purified and its glycosylation status was determined. It was found that both subunits (α and β) of the enzyme were N-glycosylated, while the β-subunit also contained O-glycans. It was also observed that rPACP.rett. was stable in a wide range of pH, which, in addition to the previously proved high thermostability, makes it an attractive biocatalyst from an industrial point of view.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Glikozilacija i pH stabilnost penicilin G acilaze iz providencia rettgeri proizvedene u Pichia pastoris
T1  - Glycosylation and pH stability of penicillin G acylase from providencia rettgeri produced in Pichia pastoris
EP  - 586
IS  - 4
SP  - 581
VL  - 61
DO  - 10.2298/ABS0904581S
ER  - 

@article{
author = "Šenerović, Lidija and Stanković, Nada and Ljubijankić, G. and Vasiljević, Branka",
year = "2009",
abstract = "Penicilin G acilaza (PAC) je jedan od najšire korišćenih enzima u industrijskoj sintezi polusintetskih antibiotika. U ovom radu dobijeni nivo ekspresije PAC gena iz Providencia rettgeri u ekspresionom sistemu Pichia pastoris iznosio je 2.7 U/ml. Rekombinantni enzim je prečišćen i određen je njegov glikozilacioni status. Nađeno je da osim što su obe subjedinice enzima (α i β) N-glikozilovane, β subjedinica sadrži još i O-glikane. Takođe je ustanovljeno da je rekombinantna PACP. rett. stabilna u širokom pH opsegu što ju je, zajedno sa predhodno ustanovljenom visokom termostabilnošću, učinilo izuzetno privlačnim biokatalizatorom sa industrijske tačke gledišta., Penicillin G acylase (PAC) is one of the most widely used enzymes in industrial synthesis of semi-synthetic antibiotics. The Providencia rettgeri pac gene was expressed to a level of 2.7 U/ml using the Pichia pastoris expression system. The recombinant enzyme was purified and its glycosylation status was determined. It was found that both subunits (α and β) of the enzyme were N-glycosylated, while the β-subunit also contained O-glycans. It was also observed that rPACP.rett. was stable in a wide range of pH, which, in addition to the previously proved high thermostability, makes it an attractive biocatalyst from an industrial point of view.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Glikozilacija i pH stabilnost penicilin G acilaze iz providencia rettgeri proizvedene u Pichia pastoris, Glycosylation and pH stability of penicillin G acylase from providencia rettgeri produced in Pichia pastoris",
pages = "586-581",
number = "4",
volume = "61",
doi = "10.2298/ABS0904581S"
}

Šenerović, L., Stanković, N., Ljubijankić, G.,& Vasiljević, B.. (2009). Glikozilacija i pH stabilnost penicilin G acilaze iz providencia rettgeri proizvedene u Pichia pastoris. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 61(4), 581-586.
https://doi.org/10.2298/ABS0904581S

Šenerović L, Stanković N, Ljubijankić G, Vasiljević B. Glikozilacija i pH stabilnost penicilin G acilaze iz providencia rettgeri proizvedene u Pichia pastoris. in Archives of Biological Sciences. 2009;61(4):581-586.
doi:10.2298/ABS0904581S .

Šenerović, Lidija, Stanković, Nada, Ljubijankić, G., Vasiljević, Branka, "Glikozilacija i pH stabilnost penicilin G acilaze iz providencia rettgeri proizvedene u Pichia pastoris" in Archives of Biological Sciences, 61, no. 4 (2009):581-586,
https://doi.org/10.2298/ABS0904581S . .

TY  - JOUR
AU  - Stanković, Nada
AU  - Vasiljević, Branka
AU  - Ljubijankić, Goran
PY  - 2007
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/282
AB  - U ovom radu opisan je uticaj gena za kanamicinsku rezistenciju (Kmr) na održavanje 2μm plazmida u Saccharomyces cerevisiae. Prisustvo ovog marker gena dovodi do gubitka stabilnog model-vektora konstantnom stopom nezavisnom od izvora ugljenika i stope rasta kulture. Kod sojeva za sintezu heterolognih proteina sa galaktoznog promotora (GALUAS) uvođenje Kmr rezultira "čišćenjem" od kvaščevih epizomalnih plazmida (YEp) u svega nekoliko generacija. Primena selektivnog pritiska na sojeve koji proizvode rekombinantnu penicilin G amidazu (rPGA) nije dovela do očekivanog povećanja prinosa proteina. Ispitivanjem uticaja samih genetičkih elemenata za proizvodnju heterolognih proteina na stabilnost vektora pokazano je da najjače destabilišuće dejstvo ima prisustvo i eksprecija stranog gena.
AB  - In this paper we describe the effect of the kanamycin resistance gene (Kmr) on 2μm-based plasmid maintenance in Saccharomyces cerevisiae. The influence of this marker gene on the loss of the stable model-vectors proved to be constant, as well as independent of carbon source and culture growth rates. In strains for GALUAS - driven heterologous protein production introduction of Kmr resulted in curing of the yeast episomal plasmid (YEp) from the population in a small number of generations. Application of selective pressure on the strain producing recombinant penicillin G amidase (rPGA) did not provide the expected increase of protein yield. The influence of genetic elements for heterologous protein production on vector stability was examined, and the most destabilizing factors prove to be the presence and expression of the foreign gene.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Uticaj marker gena za kanamicinsku rezistenciju na stabilnost 2μ ekspresionih plazmida
T1  - Effect of the kanamycin resistance marker on stability of 2μ-based expression plasmids
EP  - 12
IS  - 1
SP  - 1
VL  - 59
DO  - 10.2298/ABS0701001S
ER  - 

@article{
author = "Stanković, Nada and Vasiljević, Branka and Ljubijankić, Goran",
year = "2007",
abstract = "U ovom radu opisan je uticaj gena za kanamicinsku rezistenciju (Kmr) na održavanje 2μm plazmida u Saccharomyces cerevisiae. Prisustvo ovog marker gena dovodi do gubitka stabilnog model-vektora konstantnom stopom nezavisnom od izvora ugljenika i stope rasta kulture. Kod sojeva za sintezu heterolognih proteina sa galaktoznog promotora (GALUAS) uvođenje Kmr rezultira "čišćenjem" od kvaščevih epizomalnih plazmida (YEp) u svega nekoliko generacija. Primena selektivnog pritiska na sojeve koji proizvode rekombinantnu penicilin G amidazu (rPGA) nije dovela do očekivanog povećanja prinosa proteina. Ispitivanjem uticaja samih genetičkih elemenata za proizvodnju heterolognih proteina na stabilnost vektora pokazano je da najjače destabilišuće dejstvo ima prisustvo i eksprecija stranog gena., In this paper we describe the effect of the kanamycin resistance gene (Kmr) on 2μm-based plasmid maintenance in Saccharomyces cerevisiae. The influence of this marker gene on the loss of the stable model-vectors proved to be constant, as well as independent of carbon source and culture growth rates. In strains for GALUAS - driven heterologous protein production introduction of Kmr resulted in curing of the yeast episomal plasmid (YEp) from the population in a small number of generations. Application of selective pressure on the strain producing recombinant penicillin G amidase (rPGA) did not provide the expected increase of protein yield. The influence of genetic elements for heterologous protein production on vector stability was examined, and the most destabilizing factors prove to be the presence and expression of the foreign gene.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Uticaj marker gena za kanamicinsku rezistenciju na stabilnost 2μ ekspresionih plazmida, Effect of the kanamycin resistance marker on stability of 2μ-based expression plasmids",
pages = "12-1",
number = "1",
volume = "59",
doi = "10.2298/ABS0701001S"
}

Stanković, N., Vasiljević, B.,& Ljubijankić, G.. (2007). Uticaj marker gena za kanamicinsku rezistenciju na stabilnost 2μ ekspresionih plazmida. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 59(1), 1-12.
https://doi.org/10.2298/ABS0701001S

Stanković N, Vasiljević B, Ljubijankić G. Uticaj marker gena za kanamicinsku rezistenciju na stabilnost 2μ ekspresionih plazmida. in Archives of Biological Sciences. 2007;59(1):1-12.
doi:10.2298/ABS0701001S .

Stanković, Nada, Vasiljević, Branka, Ljubijankić, Goran, "Uticaj marker gena za kanamicinsku rezistenciju na stabilnost 2μ ekspresionih plazmida" in Archives of Biological Sciences, 59, no. 1 (2007):1-12,
https://doi.org/10.2298/ABS0701001S . .

TY  - JOUR
AU  - Šenerović, Lidija
AU  - Stanković, Nada
AU  - Spizzo, P
AU  - Basso, A
AU  - Gardossi, L
AU  - Vasiljević, Branka
AU  - Ljubijankić, G
AU  - Tisminetzky, S
AU  - Degrassi, G
PY  - 2006
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/255
AB  - A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri (rPAC(P).(rett)) of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, was constructed. The proteinase activity of the recombinant strain was reduced by knockout of the PEN gene encoding for proteinase A, resulting in an increased rPAC(P.rett) activity of approximately 40% (3.8 U/mL vs. 2.7 U/mL produced by LN5.5 in flask). A high cell density fermentation process was established with a 5-day methanol induction phase and a final PAC activity of up to 27 U/mL. A single step rPAC(P.rett) purification was also developed with an enzyme activity yield of approximately 95%. The novel features of the rPAC(P.rett) expressed in P. pastoris were fully exploited and emphasized through the covalent immobilization of rPAC(P.rett). The enzyme was immobilized on a series of structurally correlated methacrylic polymers, specifically designed and produced for optimizing rPAC(P.rett) performances in both hydrolytic and synthetic processes. Polymers presenting aminic functionalities were the most efficient, leading to formulations with higher activity and stability (half time stability  gt  3 years and specific activity ranging from 237 to 477 U/g (dry) based on benzylpenicillin hydrolysis). The efficiency of the immobilized rPAC(P.rett) was finally evaluated by studying the kinetically controlled synthesis of P-lactam antibiotics (cephalexin) and estimating the synthesis/hydrolysis ratio (S/H), which is a crucial parameter for the feasibility of the process.
PB  - Wiley, Hoboken
T2  - Biotechnology and Bioengineering
T1  - High-level production and covalent immobilization of Providencia rettgeri penicillin G acylase (PAC) from recombinant Pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability
EP  - 354
IS  - 2
SP  - 344
VL  - 93
DO  - 10.1002/bit.20728
ER  - 

@article{
author = "Šenerović, Lidija and Stanković, Nada and Spizzo, P and Basso, A and Gardossi, L and Vasiljević, Branka and Ljubijankić, G and Tisminetzky, S and Degrassi, G",
year = "2006",
abstract = "A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri (rPAC(P).(rett)) of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, was constructed. The proteinase activity of the recombinant strain was reduced by knockout of the PEN gene encoding for proteinase A, resulting in an increased rPAC(P.rett) activity of approximately 40% (3.8 U/mL vs. 2.7 U/mL produced by LN5.5 in flask). A high cell density fermentation process was established with a 5-day methanol induction phase and a final PAC activity of up to 27 U/mL. A single step rPAC(P.rett) purification was also developed with an enzyme activity yield of approximately 95%. The novel features of the rPAC(P.rett) expressed in P. pastoris were fully exploited and emphasized through the covalent immobilization of rPAC(P.rett). The enzyme was immobilized on a series of structurally correlated methacrylic polymers, specifically designed and produced for optimizing rPAC(P.rett) performances in both hydrolytic and synthetic processes. Polymers presenting aminic functionalities were the most efficient, leading to formulations with higher activity and stability (half time stability  gt  3 years and specific activity ranging from 237 to 477 U/g (dry) based on benzylpenicillin hydrolysis). The efficiency of the immobilized rPAC(P.rett) was finally evaluated by studying the kinetically controlled synthesis of P-lactam antibiotics (cephalexin) and estimating the synthesis/hydrolysis ratio (S/H), which is a crucial parameter for the feasibility of the process.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Bioengineering",
title = "High-level production and covalent immobilization of Providencia rettgeri penicillin G acylase (PAC) from recombinant Pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability",
pages = "354-344",
number = "2",
volume = "93",
doi = "10.1002/bit.20728"
}

Šenerović, L., Stanković, N., Spizzo, P., Basso, A., Gardossi, L., Vasiljević, B., Ljubijankić, G., Tisminetzky, S.,& Degrassi, G.. (2006). High-level production and covalent immobilization of Providencia rettgeri penicillin G acylase (PAC) from recombinant Pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability. in Biotechnology and Bioengineering
Wiley, Hoboken., 93(2), 344-354.
https://doi.org/10.1002/bit.20728

Šenerović L, Stanković N, Spizzo P, Basso A, Gardossi L, Vasiljević B, Ljubijankić G, Tisminetzky S, Degrassi G. High-level production and covalent immobilization of Providencia rettgeri penicillin G acylase (PAC) from recombinant Pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability. in Biotechnology and Bioengineering. 2006;93(2):344-354.
doi:10.1002/bit.20728 .

Šenerović, Lidija, Stanković, Nada, Spizzo, P, Basso, A, Gardossi, L, Vasiljević, Branka, Ljubijankić, G, Tisminetzky, S, Degrassi, G, "High-level production and covalent immobilization of Providencia rettgeri penicillin G acylase (PAC) from recombinant Pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability" in Biotechnology and Bioengineering, 93, no. 2 (2006):344-354,
https://doi.org/10.1002/bit.20728 . .