TY  - JOUR
AU  - Pereira, Tanya
AU  - Nikodinović-Runić, Jasmina
AU  - Nakazono, Chojin
AU  - Dennis, Gary R.
AU  - Barrow, Kevin D.
AU  - Chuck, Jo-Anne
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/339
AB  - Immobilized bacteria are being assessed by industry for drug delivery, novel fermentation systems and the protection of organisms in harsh environments. Alginate bioreactors containing Streptomyces nodosus were examined for community structure, cell viability and amphotericin production under different growth conditions. When cell proliferation was encouraged, substrate hyphae were found inside the alginate matrix and within multicellular projections on the surface of the capsule. The periphery of these projections had erect and branched hyphae, morphologically identical to aerial hyphae. Antibiotic production from immobilized organisms was assessed using conditioned culture medium to eliminate the emergence of a free-dwelling population. These organisms sporulated with reduced antibiotic production compared with free-dwelling cultures. The commitment to sporulate was independent of a surface but dependent on community size and nutritional status. This is the first report of the sporulation of S. nodosus in liquid cultures and description of the multicellular community the organism adopts at a solid-liquid interface.
PB  - Wiley, Hoboken
T2  - Microbial Biotechnology
T1  - Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments
EP  - 381
IS  - 5
SP  - 373
VL  - 1
DO  - 10.1111/j.1751-7915.2008.00032.x
ER  - 

@article{
author = "Pereira, Tanya and Nikodinović-Runić, Jasmina and Nakazono, Chojin and Dennis, Gary R. and Barrow, Kevin D. and Chuck, Jo-Anne",
year = "2008",
abstract = "Immobilized bacteria are being assessed by industry for drug delivery, novel fermentation systems and the protection of organisms in harsh environments. Alginate bioreactors containing Streptomyces nodosus were examined for community structure, cell viability and amphotericin production under different growth conditions. When cell proliferation was encouraged, substrate hyphae were found inside the alginate matrix and within multicellular projections on the surface of the capsule. The periphery of these projections had erect and branched hyphae, morphologically identical to aerial hyphae. Antibiotic production from immobilized organisms was assessed using conditioned culture medium to eliminate the emergence of a free-dwelling population. These organisms sporulated with reduced antibiotic production compared with free-dwelling cultures. The commitment to sporulate was independent of a surface but dependent on community size and nutritional status. This is the first report of the sporulation of S. nodosus in liquid cultures and description of the multicellular community the organism adopts at a solid-liquid interface.",
publisher = "Wiley, Hoboken",
journal = "Microbial Biotechnology",
title = "Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments",
pages = "381-373",
number = "5",
volume = "1",
doi = "10.1111/j.1751-7915.2008.00032.x"
}

Pereira, T., Nikodinović-Runić, J., Nakazono, C., Dennis, G. R., Barrow, K. D.,& Chuck, J.. (2008). Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments. in Microbial Biotechnology
Wiley, Hoboken., 1(5), 373-381.
https://doi.org/10.1111/j.1751-7915.2008.00032.x

Pereira T, Nikodinović-Runić J, Nakazono C, Dennis GR, Barrow KD, Chuck J. Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments. in Microbial Biotechnology. 2008;1(5):373-381.
doi:10.1111/j.1751-7915.2008.00032.x .

Pereira, Tanya, Nikodinović-Runić, Jasmina, Nakazono, Chojin, Dennis, Gary R., Barrow, Kevin D., Chuck, Jo-Anne, "Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments" in Microbial Biotechnology, 1, no. 5 (2008):373-381,
https://doi.org/10.1111/j.1751-7915.2008.00032.x . .

TY  - JOUR
AU  - Chuck, Jo-Anne
AU  - Dunn, Catherine
AU  - Facultad, Fe E. C. D.
AU  - Nakazono, Chojin
AU  - Nikodinović-Runić, Jasmina
AU  - Barrow, Kevin D.
PY  - 2006
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/251
AB  - Polyketides are a group of bioactive compounds from bacteria, plants, and fungi. To increase the availability of analogs for testing, the active sites of polyketide synthases are often substituted with homologous domains having altered substrate specificities. This study reports the design of polymerase chain reaction primers that enables isolation of entire active site domains from type I polyketide synthases with native interdomain linkers. This bypasses the need for further genetic screening to obtain functional units for use in genetic engineering. This is especially important in bioprospecting projects exploring new environments for bioresources.
PB  - Springer, New York
T2  - Current Microbiology
T1  - Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species
EP  - 94
IS  - 2
SP  - 89
VL  - 53
DO  - 10.1007/s00284-005-0050-x
ER  - 

@article{
author = "Chuck, Jo-Anne and Dunn, Catherine and Facultad, Fe E. C. D. and Nakazono, Chojin and Nikodinović-Runić, Jasmina and Barrow, Kevin D.",
year = "2006",
abstract = "Polyketides are a group of bioactive compounds from bacteria, plants, and fungi. To increase the availability of analogs for testing, the active sites of polyketide synthases are often substituted with homologous domains having altered substrate specificities. This study reports the design of polymerase chain reaction primers that enables isolation of entire active site domains from type I polyketide synthases with native interdomain linkers. This bypasses the need for further genetic screening to obtain functional units for use in genetic engineering. This is especially important in bioprospecting projects exploring new environments for bioresources.",
publisher = "Springer, New York",
journal = "Current Microbiology",
title = "Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species",
pages = "94-89",
number = "2",
volume = "53",
doi = "10.1007/s00284-005-0050-x"
}

Chuck, J., Dunn, C., Facultad, F. E. C. D., Nakazono, C., Nikodinović-Runić, J.,& Barrow, K. D.. (2006). Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species. in Current Microbiology
Springer, New York., 53(2), 89-94.
https://doi.org/10.1007/s00284-005-0050-x

Chuck J, Dunn C, Facultad FECD, Nakazono C, Nikodinović-Runić J, Barrow KD. Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species. in Current Microbiology. 2006;53(2):89-94.
doi:10.1007/s00284-005-0050-x .

Chuck, Jo-Anne, Dunn, Catherine, Facultad, Fe E. C. D., Nakazono, Chojin, Nikodinović-Runić, Jasmina, Barrow, Kevin D., "Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species" in Current Microbiology, 53, no. 2 (2006):89-94,
https://doi.org/10.1007/s00284-005-0050-x . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Barrow, Kevin D.
AU  - Chuck, JA
PY  - 2003
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/184
PB  - Eaton Publishing Co, Natick
T2  - Biotechniques
T1  - High yield preparation of genomic DNA from Streptomyces
EP  - +
IS  - 5
SP  - 932
VL  - 35
DO  - 10.2144/03355bm05
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Barrow, Kevin D. and Chuck, JA",
year = "2003",
publisher = "Eaton Publishing Co, Natick",
journal = "Biotechniques",
title = "High yield preparation of genomic DNA from Streptomyces",
pages = "+-932",
number = "5",
volume = "35",
doi = "10.2144/03355bm05"
}

Nikodinović-Runić, J., Barrow, K. D.,& Chuck, J.. (2003). High yield preparation of genomic DNA from Streptomyces. in Biotechniques
Eaton Publishing Co, Natick., 35(5), 932-+.
https://doi.org/10.2144/03355bm05

Nikodinović-Runić J, Barrow KD, Chuck J. High yield preparation of genomic DNA from Streptomyces. in Biotechniques. 2003;35(5):932-+.
doi:10.2144/03355bm05 .

Nikodinović-Runić, Jasmina, Barrow, Kevin D., Chuck, JA, "High yield preparation of genomic DNA from Streptomyces" in Biotechniques, 35, no. 5 (2003):932-+,
https://doi.org/10.2144/03355bm05 . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Barrow, Kevin D.
AU  - Chuck, JA
PY  - 2003
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/181
AB  - This study has investigated DNA transformation in the Amphotericin-producing organism Streptomyces nodosus. Amphotericin B is an antifungal drug with severe side effects in humans and the availability of structural variants would aid investigations into the mode of action and cytotoxity of the drug. Analogs of related polyketide drugs have been rapidly made by genetic engineering of biosynthetic genes; however, this requires the introduction of foreign DNA into the host. Protocols for protoplast formation and regeneration were established; however, preparations were recalcitrant to DNA uptake. Electroporation-mediated methodologies also were not successful. Intergeneric conjugal transfer of DNA from E. coli demonstrated transformation efficiencies of 5 x 10(-5) exconjugants generated per recipient. Use of DNA methylation-impaired E. coli donor strains resulted in 100-fold higher transformation efficiencies, indicating that DNA methylation recognition systems are operable in the organism. This methodology will enable genetic and biochemical analysis of the gene cluster responsible for making Amphotericin B.
PB  - Elsevier, Amsterdam
T2  - Journal of Microbiological Methods
T1  - High frequency transformation of the amphotericin-producing bacterium Streptomyces nodosus
EP  - 277
IS  - 1
SP  - 273
VL  - 55
DO  - 10.1016/S0167-7012(03)00160-X
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Barrow, Kevin D. and Chuck, JA",
year = "2003",
abstract = "This study has investigated DNA transformation in the Amphotericin-producing organism Streptomyces nodosus. Amphotericin B is an antifungal drug with severe side effects in humans and the availability of structural variants would aid investigations into the mode of action and cytotoxity of the drug. Analogs of related polyketide drugs have been rapidly made by genetic engineering of biosynthetic genes; however, this requires the introduction of foreign DNA into the host. Protocols for protoplast formation and regeneration were established; however, preparations were recalcitrant to DNA uptake. Electroporation-mediated methodologies also were not successful. Intergeneric conjugal transfer of DNA from E. coli demonstrated transformation efficiencies of 5 x 10(-5) exconjugants generated per recipient. Use of DNA methylation-impaired E. coli donor strains resulted in 100-fold higher transformation efficiencies, indicating that DNA methylation recognition systems are operable in the organism. This methodology will enable genetic and biochemical analysis of the gene cluster responsible for making Amphotericin B.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Microbiological Methods",
title = "High frequency transformation of the amphotericin-producing bacterium Streptomyces nodosus",
pages = "277-273",
number = "1",
volume = "55",
doi = "10.1016/S0167-7012(03)00160-X"
}

Nikodinović-Runić, J., Barrow, K. D.,& Chuck, J.. (2003). High frequency transformation of the amphotericin-producing bacterium Streptomyces nodosus. in Journal of Microbiological Methods
Elsevier, Amsterdam., 55(1), 273-277.
https://doi.org/10.1016/S0167-7012(03)00160-X

Nikodinović-Runić J, Barrow KD, Chuck J. High frequency transformation of the amphotericin-producing bacterium Streptomyces nodosus. in Journal of Microbiological Methods. 2003;55(1):273-277.
doi:10.1016/S0167-7012(03)00160-X .

Nikodinović-Runić, Jasmina, Barrow, Kevin D., Chuck, JA, "High frequency transformation of the amphotericin-producing bacterium Streptomyces nodosus" in Journal of Microbiological Methods, 55, no. 1 (2003):273-277,
https://doi.org/10.1016/S0167-7012(03)00160-X . .