TY  - JOUR
AU  - Petković, Anica
AU  - Al-Khalili, Faris
AU  - Antović, Aleksandra
AU  - Ammar, Majeed
AU  - Pruner, Iva
AU  - Vranić, Aleksandra
AU  - Soutari, Nida
AU  - Zdravković, Nebojša
AU  - Malmstrom, Rickard E.
AU  - Jakovljević, Vladimir
AU  - Antović, Jovan P.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1345
AB  - The study was aimed to evaluate the effects of two standard doses of rivaroxaban and dabigatran on global hemostatic assays in patients with atrial fibrillation. The study included 52 patients treated with rivaroxaban (15/20 mg), 50 on dabigatran (110/150 mg) and 20 healthy individuals. Platelet-poor plasma was used for determination of three global hemostatic assays, namely endogenous thrombin potential (ETP), calibrated automated thrombogram (CAT) and overall hemostasis potential (OHP). Rivaroxaban and dabigatran reduced ETP (P lt 0.01) although OHP (P lt 0.05) was diminished only by dabigatran. Strong correlations were noticed between ETP parameters and the plasma concentrations of rivaroxaban (ETP, rUS0.51; c-max, rUS0.85; t-lag, rU0.83; t-max, rU0.66) as well as with plasma concentration of dabigatran (ETP, rUS0.75; c-max, rUS0.74; t-lag, rU0.73; t-max, rU0.52). Analysis of dabigatran concentrations under 50 ng/ml showed that ETP parameter has area under the concentration-time curvereceiver operating characteristic value of 0.879 (95% confidence interval 0.776-0.980). Dabigatran treatment paradoxically increased area under the concentration-time curve and peak values although rivaroxaban decreased peak values (P lt 0.01). However, significant correlation between CAT parameters and plasma concentration of both direct oral anticoagulants was not observed. We confirmed that the CAT assay is inappropriate for estimation of dabigatran effects and is not fully sensitive as regards rivaroxaban. The ETP assay can potentially be the appropriate method for estimation of global hemostatic capacity as regards both direct oral anticoagulants. The role of OHP needs to be confirmed in additional studies. ETP parameter of chromogenic assay has promising potential in exclusion of high plasma concentrations of dabigatran.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Blood Coagulation & Fibrinolysis
T1  - Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation
EP  - 252
IS  - 4
SP  - 243
VL  - 31
DO  - 10.1097/MBC.0000000000000907
ER  - 

@article{
author = "Petković, Anica and Al-Khalili, Faris and Antović, Aleksandra and Ammar, Majeed and Pruner, Iva and Vranić, Aleksandra and Soutari, Nida and Zdravković, Nebojša and Malmstrom, Rickard E. and Jakovljević, Vladimir and Antović, Jovan P.",
year = "2020",
abstract = "The study was aimed to evaluate the effects of two standard doses of rivaroxaban and dabigatran on global hemostatic assays in patients with atrial fibrillation. The study included 52 patients treated with rivaroxaban (15/20 mg), 50 on dabigatran (110/150 mg) and 20 healthy individuals. Platelet-poor plasma was used for determination of three global hemostatic assays, namely endogenous thrombin potential (ETP), calibrated automated thrombogram (CAT) and overall hemostasis potential (OHP). Rivaroxaban and dabigatran reduced ETP (P lt 0.01) although OHP (P lt 0.05) was diminished only by dabigatran. Strong correlations were noticed between ETP parameters and the plasma concentrations of rivaroxaban (ETP, rUS0.51; c-max, rUS0.85; t-lag, rU0.83; t-max, rU0.66) as well as with plasma concentration of dabigatran (ETP, rUS0.75; c-max, rUS0.74; t-lag, rU0.73; t-max, rU0.52). Analysis of dabigatran concentrations under 50 ng/ml showed that ETP parameter has area under the concentration-time curvereceiver operating characteristic value of 0.879 (95% confidence interval 0.776-0.980). Dabigatran treatment paradoxically increased area under the concentration-time curve and peak values although rivaroxaban decreased peak values (P lt 0.01). However, significant correlation between CAT parameters and plasma concentration of both direct oral anticoagulants was not observed. We confirmed that the CAT assay is inappropriate for estimation of dabigatran effects and is not fully sensitive as regards rivaroxaban. The ETP assay can potentially be the appropriate method for estimation of global hemostatic capacity as regards both direct oral anticoagulants. The role of OHP needs to be confirmed in additional studies. ETP parameter of chromogenic assay has promising potential in exclusion of high plasma concentrations of dabigatran.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Blood Coagulation & Fibrinolysis",
title = "Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation",
pages = "252-243",
number = "4",
volume = "31",
doi = "10.1097/MBC.0000000000000907"
}

Petković, A., Al-Khalili, F., Antović, A., Ammar, M., Pruner, I., Vranić, A., Soutari, N., Zdravković, N., Malmstrom, R. E., Jakovljević, V.,& Antović, J. P.. (2020). Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation. in Blood Coagulation & Fibrinolysis
Lippincott Williams & Wilkins, Philadelphia., 31(4), 243-252.
https://doi.org/10.1097/MBC.0000000000000907

Petković A, Al-Khalili F, Antović A, Ammar M, Pruner I, Vranić A, Soutari N, Zdravković N, Malmstrom RE, Jakovljević V, Antović JP. Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation. in Blood Coagulation & Fibrinolysis. 2020;31(4):243-252.
doi:10.1097/MBC.0000000000000907 .

Petković, Anica, Al-Khalili, Faris, Antović, Aleksandra, Ammar, Majeed, Pruner, Iva, Vranić, Aleksandra, Soutari, Nida, Zdravković, Nebojša, Malmstrom, Rickard E., Jakovljević, Vladimir, Antović, Jovan P., "Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation" in Blood Coagulation & Fibrinolysis, 31, no. 4 (2020):243-252,
https://doi.org/10.1097/MBC.0000000000000907 . .

TY  - JOUR
AU  - Vranić, Aleksandra
AU  - Pruner, Iva
AU  - Veselinović, Mirjana
AU  - Soutari, Nida
AU  - Petković, Anica
AU  - Jakovljević, Vladimir
AU  - Antović, Aleksandra
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1230
AB  - Objectives This study was aimed to assess hemostatic disturbances in female patients with established rheumatoid arthritis (RA) in relation to menopausal status and disease activity. Method Ninety women were included in the study, 42 patients and 48 age-matched healthy controls. There were no differences between the investigated groups regarding the presence of traditional cardiovascular risk factors. Two global hemostatic assays were employed, namely endogenous thrombin potential (ETP) and overall hemostasis potential (OHP). The parameters of the ETP assay (ETP, C-max, t-lag, t-max) and OHP assay (overall coagulation potential (OCP) and overall fibrinolytic potential (OFP)) were assessed. Moreover, the parameters of the fibrin clot (lag time, Max Abs, and slope) were measured by clot turbidity and scanning electron microscopy (SEM). Both patients and controls were divided into four subgroups according to menopause status. Results The premenopausal controls differed significantly from all other subgroups in terms of diminished levels of ETP (p = 0.02), C-max (p = 0.01), OCP (p = 0.02), OHP (p = 0.001), and Max Abs (p = 0.008), while OFP (p = 0.0001) was increased. This tendency was not seen in the premenopausal RA patients compared with the postmenopausal RA patients. SEM images showed denser clots composed of thinner fibers in samples from RA patients. The disease activity measured by DAS28 correlated with OCP and OHP (r = 0.54; p = 0.001 and r = 0.44; p = 0.003, respectively) indicating persistent hypercoagulable condition in the whole group of RA patients. Conclusions Our results point towards coagulation activation in premenopausal women with established RA. The patients were well characterized, which enabled assessment in a real-life setting.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Assessment of hemostatic disturbances in women with established rheumatoid arthritis
EP  - 3014
IS  - 11
SP  - 3005
VL  - 38
DO  - 10.1007/s10067-019-04629-8
ER  - 

@article{
author = "Vranić, Aleksandra and Pruner, Iva and Veselinović, Mirjana and Soutari, Nida and Petković, Anica and Jakovljević, Vladimir and Antović, Aleksandra",
year = "2019",
abstract = "Objectives This study was aimed to assess hemostatic disturbances in female patients with established rheumatoid arthritis (RA) in relation to menopausal status and disease activity. Method Ninety women were included in the study, 42 patients and 48 age-matched healthy controls. There were no differences between the investigated groups regarding the presence of traditional cardiovascular risk factors. Two global hemostatic assays were employed, namely endogenous thrombin potential (ETP) and overall hemostasis potential (OHP). The parameters of the ETP assay (ETP, C-max, t-lag, t-max) and OHP assay (overall coagulation potential (OCP) and overall fibrinolytic potential (OFP)) were assessed. Moreover, the parameters of the fibrin clot (lag time, Max Abs, and slope) were measured by clot turbidity and scanning electron microscopy (SEM). Both patients and controls were divided into four subgroups according to menopause status. Results The premenopausal controls differed significantly from all other subgroups in terms of diminished levels of ETP (p = 0.02), C-max (p = 0.01), OCP (p = 0.02), OHP (p = 0.001), and Max Abs (p = 0.008), while OFP (p = 0.0001) was increased. This tendency was not seen in the premenopausal RA patients compared with the postmenopausal RA patients. SEM images showed denser clots composed of thinner fibers in samples from RA patients. The disease activity measured by DAS28 correlated with OCP and OHP (r = 0.54; p = 0.001 and r = 0.44; p = 0.003, respectively) indicating persistent hypercoagulable condition in the whole group of RA patients. Conclusions Our results point towards coagulation activation in premenopausal women with established RA. The patients were well characterized, which enabled assessment in a real-life setting.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Assessment of hemostatic disturbances in women with established rheumatoid arthritis",
pages = "3014-3005",
number = "11",
volume = "38",
doi = "10.1007/s10067-019-04629-8"
}

Vranić, A., Pruner, I., Veselinović, M., Soutari, N., Petković, A., Jakovljević, V.,& Antović, A.. (2019). Assessment of hemostatic disturbances in women with established rheumatoid arthritis. in Clinical Rheumatology
Springer London Ltd, London., 38(11), 3005-3014.
https://doi.org/10.1007/s10067-019-04629-8

Vranić A, Pruner I, Veselinović M, Soutari N, Petković A, Jakovljević V, Antović A. Assessment of hemostatic disturbances in women with established rheumatoid arthritis. in Clinical Rheumatology. 2019;38(11):3005-3014.
doi:10.1007/s10067-019-04629-8 .

Vranić, Aleksandra, Pruner, Iva, Veselinović, Mirjana, Soutari, Nida, Petković, Anica, Jakovljević, Vladimir, Antović, Aleksandra, "Assessment of hemostatic disturbances in women with established rheumatoid arthritis" in Clinical Rheumatology, 38, no. 11 (2019):3005-3014,
https://doi.org/10.1007/s10067-019-04629-8 . .