TY  - JOUR
AU  - Dumanović, Jelena
AU  - Kolarž, Predrag
AU  - Sedlarević Zorić, Ana
AU  - Morina, Filis
AU  - Vidović, Marija
AU  - Veljović Jovanović, Sonja
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/20/15350
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2170
AB  - Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum × citriodorum, and Ocimum basilicum var. purpurascens) were grown under moderate light (about 300 µmol photons m−2 s−1) in a glasshouse or growth chamber and then either transferred to an open field (average daily dose: 29.2 kJ m−2 d−1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m−2 d−1), to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity (TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity, but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance mechanism to high doses of UV-B irradiation.
T2  - International Journal of Molecular Sciences
T1  - Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties
IS  - 20
SP  - 15350
VL  - 24
DO  - 10.3390/ijms242015350
ER  - 

@article{
author = "Dumanović, Jelena and Kolarž, Predrag and Sedlarević Zorić, Ana and Morina, Filis and Vidović, Marija and Veljović Jovanović, Sonja",
year = "2023",
abstract = "Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum × citriodorum, and Ocimum basilicum var. purpurascens) were grown under moderate light (about 300 µmol photons m−2 s−1) in a glasshouse or growth chamber and then either transferred to an open field (average daily dose: 29.2 kJ m−2 d−1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m−2 d−1), to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity (TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity, but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance mechanism to high doses of UV-B irradiation.",
journal = "International Journal of Molecular Sciences",
title = "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties",
number = "20",
pages = "15350",
volume = "24",
doi = "10.3390/ijms242015350"
}

Dumanović, J., Kolarž, P., Sedlarević Zorić, A., Morina, F., Vidović, M.,& Veljović Jovanović, S.. (2023). Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences, 24(20), 15350.
https://doi.org/10.3390/ijms242015350

Dumanović J, Kolarž P, Sedlarević Zorić A, Morina F, Vidović M, Veljović Jovanović S. Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences. 2023;24(20):15350.
doi:10.3390/ijms242015350 .

Dumanović, Jelena, Kolarž, Predrag, Sedlarević Zorić, Ana, Morina, Filis, Vidović, Marija, Veljović Jovanović, Sonja, "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties" in International Journal of Molecular Sciences, 24, no. 20 (2023):15350,
https://doi.org/10.3390/ijms242015350 . .

TY  - CONF
AU  - Pantelić, Ana
AU  - Prodić, Ivana
AU  - Milić, Dejana
AU  - Senćanski, Milan
AU  - Vidović, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2149
AB  - Introduction: Resurrection plants (such as Ramonda serbica) can survive a long desiccation period and
fully resume their metabolism upon watering. The hallmark of desiccation tolerance (DT) is the accumulation of protective, intrinsically disordered proteins(IDPs), called late embryogenesis abundant proteins (LEAPs). Although their high structural plasticity allows them to interact with various partners, no
specific cellular targets of LEAPs have been identified so far.
Methods: To identify LEAPsinvolved in DT, differential transcriptome and proteome analyses of hydrated
and desiccated R. serbica leaves were performed. The identified LEAPs were structurally characterised
and classified. To evaluate theirstructural propertiesin vitro and their potential functionsin vivo, the representative RsLEA proteins, were produced in Escherichia coli using recombinant DNA technology.
Results: Members of the LEA4 protein family represent the majority of desiccation-inducible LEAPs. Even
17 proteins belonging to the LEA4 protein family group were induced by desiccation. They show high disorder propensity (82 %), and at the same time, a high tendency to form α-helices (>80%). Although recombinant DNA technology has traditionally been used to overexpress and purify various globular
proteins, the production of IDPsis challenging due to their high susceptibility to proteolytic cleavage and
aggregation. Nevertheless, the representative LEAPs containing hexa-Histagsimmunoglobulin G-binding protein and a proteolytic TEV site were produced, purified and cleaved by TEV protease.
Conclusion: The combination of in silico and in vitro results will be crucial for the identification of endogenous partners of LEAPs, providing further insight into their role in DT.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica
EP  - 110
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2149
ER  - 

@conference{
author = "Pantelić, Ana and Prodić, Ivana and Milić, Dejana and Senćanski, Milan and Vidović, Marija",
year = "2023",
abstract = "Introduction: Resurrection plants (such as Ramonda serbica) can survive a long desiccation period and
fully resume their metabolism upon watering. The hallmark of desiccation tolerance (DT) is the accumulation of protective, intrinsically disordered proteins(IDPs), called late embryogenesis abundant proteins (LEAPs). Although their high structural plasticity allows them to interact with various partners, no
specific cellular targets of LEAPs have been identified so far.
Methods: To identify LEAPsinvolved in DT, differential transcriptome and proteome analyses of hydrated
and desiccated R. serbica leaves were performed. The identified LEAPs were structurally characterised
and classified. To evaluate theirstructural propertiesin vitro and their potential functionsin vivo, the representative RsLEA proteins, were produced in Escherichia coli using recombinant DNA technology.
Results: Members of the LEA4 protein family represent the majority of desiccation-inducible LEAPs. Even
17 proteins belonging to the LEA4 protein family group were induced by desiccation. They show high disorder propensity (82 %), and at the same time, a high tendency to form α-helices (>80%). Although recombinant DNA technology has traditionally been used to overexpress and purify various globular
proteins, the production of IDPsis challenging due to their high susceptibility to proteolytic cleavage and
aggregation. Nevertheless, the representative LEAPs containing hexa-Histagsimmunoglobulin G-binding protein and a proteolytic TEV site were produced, purified and cleaved by TEV protease.
Conclusion: The combination of in silico and in vitro results will be crucial for the identification of endogenous partners of LEAPs, providing further insight into their role in DT.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2149"
}

Pantelić, A., Prodić, I., Milić, D., Senćanski, M.,& Vidović, M.. (2023). Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 110-110.
https://hdl.handle.net/21.15107/rcub_imagine_2149

Pantelić A, Prodić I, Milić D, Senćanski M, Vidović M. Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:110-110.
https://hdl.handle.net/21.15107/rcub_imagine_2149 .

Pantelić, Ana, Prodić, Ivana, Milić, Dejana, Senćanski, Milan, Vidović, Marija, "Drying without dying: revealing the role of late embryogenesis abundant proteins during desiccation in Ramonda serbica" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):110-110,
https://hdl.handle.net/21.15107/rcub_imagine_2149 .

TY  - JOUR
AU  - Veljović Jovanović, Sonja
AU  - Kasalica, Bećko
AU  - Miletić, Katarina
AU  - Vidović, Marija
AU  - Šušić, Nikola
AU  - Jeremić, Dejan
AU  - Belča, Ivan
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/18/14265
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2169
AB  - Chloroplast movement rapidly ameliorates the effects of suboptimal light intensity by accumulating along the periclinal cell walls, as well as the effects of excess light by shifting to the anticlinal cell walls. These acclimation responses are triggered by phototropins located at the plasma membrane and chloroplast envelope. Here, we used a recently developed non-invasive system sensitive to very small changes in red light leaf transmittance to perform long-term continuous measurements of dark–light transitions. As a model system, we used variegated Pelargonium zonale leaves containing green sectors (GS) with fully developed chloroplasts and achlorophyllous, white sectors (WS) with undifferentiated plastids, and higher phototropin expression levels. We observed biphasic changes in the red-light transmittance and oscillations triggered by medium intensities of white light, described by a transient peak preceded by a constant decrease in transmittance level. A slight change in red-light transmittance was recorded even in WS. Furthermore, the chloroplast position at lower light intensities affected the rapid light curves, while high light intensity decreased saturated electron transport, maximum quantum efficiency of photosystem II, and increased non-photochemical quenching of chlorophyll fluorescence and epidermal flavonoids. Our results extend the knowledge of light-dependent chloroplast movements and thus contribute to a better understanding of their role in regulating photosynthesis under fluctuating light conditions.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency
IS  - 18
SP  - 14265
VL  - 24
DO  - 10.3390/ijms241814265
ER  - 

@article{
author = "Veljović Jovanović, Sonja and Kasalica, Bećko and Miletić, Katarina and Vidović, Marija and Šušić, Nikola and Jeremić, Dejan and Belča, Ivan",
year = "2023",
abstract = "Chloroplast movement rapidly ameliorates the effects of suboptimal light intensity by accumulating along the periclinal cell walls, as well as the effects of excess light by shifting to the anticlinal cell walls. These acclimation responses are triggered by phototropins located at the plasma membrane and chloroplast envelope. Here, we used a recently developed non-invasive system sensitive to very small changes in red light leaf transmittance to perform long-term continuous measurements of dark–light transitions. As a model system, we used variegated Pelargonium zonale leaves containing green sectors (GS) with fully developed chloroplasts and achlorophyllous, white sectors (WS) with undifferentiated plastids, and higher phototropin expression levels. We observed biphasic changes in the red-light transmittance and oscillations triggered by medium intensities of white light, described by a transient peak preceded by a constant decrease in transmittance level. A slight change in red-light transmittance was recorded even in WS. Furthermore, the chloroplast position at lower light intensities affected the rapid light curves, while high light intensity decreased saturated electron transport, maximum quantum efficiency of photosystem II, and increased non-photochemical quenching of chlorophyll fluorescence and epidermal flavonoids. Our results extend the knowledge of light-dependent chloroplast movements and thus contribute to a better understanding of their role in regulating photosynthesis under fluctuating light conditions.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency",
number = "18",
pages = "14265",
volume = "24",
doi = "10.3390/ijms241814265"
}

Veljović Jovanović, S., Kasalica, B., Miletić, K., Vidović, M., Šušić, N., Jeremić, D.,& Belča, I.. (2023). Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency. in International Journal of Molecular Sciences, 24(18), 14265.
https://doi.org/10.3390/ijms241814265

Veljović Jovanović S, Kasalica B, Miletić K, Vidović M, Šušić N, Jeremić D, Belča I. Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency. in International Journal of Molecular Sciences. 2023;24(18):14265.
doi:10.3390/ijms241814265 .

Veljović Jovanović, Sonja, Kasalica, Bećko, Miletić, Katarina, Vidović, Marija, Šušić, Nikola, Jeremić, Dejan, Belča, Ivan, "Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency" in International Journal of Molecular Sciences, 24, no. 18 (2023):14265,
https://doi.org/10.3390/ijms241814265 . .

TY  - CONF
AU  - Senćanski, Milan
AU  - Prodić, Ivana
AU  - Pantelić, Ana
AU  - Vidović, Marija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1997
AB  - Drought stress is one of the greatest threats to global food security, posing a major challenge
to agriculture. Understanding the molecular mechanisms underlying desiccation tolerance
in resurrection plants like Ramonda serbica Panc., can provide valuable insights for improving
crop resilience. Dehydrins are intrinsically disordered proteins known to accumulate in these
plants in response to desiccation. Among several proposed physiological roles, it has been
suggested that dehydrins can protect DNA from damage during water shortage. Here, we
have characterised dehydrins from R. serbica, selected a representative one and evaluated
its potential to interact with DNA.
Most of the R. serbica dehydrins were designated as hydrophilins (glycine content >6%;
GRAVY index <1). They exhibit a high disorder propensity, making them quite dynamic
in solution. Furthermore, they were predicted to localize in the nucleus. To examine the
potential interactions with DNA in silico, we have selected a representative, highly hydrophilic
dehydrin (Gravy index: –1.29) containing a high percentage of glycine (22.6%) and charged
amino acids (lysine, glutamate and aspartate). Its 3D structures were determined using the
Phyre 2 intensive modelling and AlphaFold.
The dehydrin-DNA complex was manually adjusted, following molecular dynamic simulation
(MDS) in both cases of hydration and desiccation. To simulate complete hydration, the DNAdehydrin
complex was solvated in a water box, with final dimensions of 100×69×82 Å,
neutralised with 0.15 M NaCl. The system underwent a 10,000-step energy minimization,
consecutive 1250 ps equilibration NVE (constant number of atoms, volume and energy)
heating from 10 K to 298 K and 100 ns NPT (constant number of atoms, pressure and
temperature) MD production at 1 bar, and 1 fs integration step. In all simulations, periodic
boundary conditions (PBC) were implemented and the CHARMM36 force field was used.
The obtained results revealed that selected dehydrin can interact with both minor and major
DNA grooves. The phosphate groups from the DNA molecule form salt bridges with the
positively charged lysines from polylysine, K-segment, contributing to the complex stability.
Overall, we have provided evidence for possible dehydrin-DNA interactions. However, the
exact nature and significance of these interactions is still an area of active research in vitro.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD)
EP  - 57
SP  - 57
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1997
ER  - 

@conference{
author = "Senćanski, Milan and Prodić, Ivana and Pantelić, Ana and Vidović, Marija",
year = "2023",
abstract = "Drought stress is one of the greatest threats to global food security, posing a major challenge
to agriculture. Understanding the molecular mechanisms underlying desiccation tolerance
in resurrection plants like Ramonda serbica Panc., can provide valuable insights for improving
crop resilience. Dehydrins are intrinsically disordered proteins known to accumulate in these
plants in response to desiccation. Among several proposed physiological roles, it has been
suggested that dehydrins can protect DNA from damage during water shortage. Here, we
have characterised dehydrins from R. serbica, selected a representative one and evaluated
its potential to interact with DNA.
Most of the R. serbica dehydrins were designated as hydrophilins (glycine content >6%;
GRAVY index <1). They exhibit a high disorder propensity, making them quite dynamic
in solution. Furthermore, they were predicted to localize in the nucleus. To examine the
potential interactions with DNA in silico, we have selected a representative, highly hydrophilic
dehydrin (Gravy index: –1.29) containing a high percentage of glycine (22.6%) and charged
amino acids (lysine, glutamate and aspartate). Its 3D structures were determined using the
Phyre 2 intensive modelling and AlphaFold.
The dehydrin-DNA complex was manually adjusted, following molecular dynamic simulation
(MDS) in both cases of hydration and desiccation. To simulate complete hydration, the DNAdehydrin
complex was solvated in a water box, with final dimensions of 100×69×82 Å,
neutralised with 0.15 M NaCl. The system underwent a 10,000-step energy minimization,
consecutive 1250 ps equilibration NVE (constant number of atoms, volume and energy)
heating from 10 K to 298 K and 100 ns NPT (constant number of atoms, pressure and
temperature) MD production at 1 bar, and 1 fs integration step. In all simulations, periodic
boundary conditions (PBC) were implemented and the CHARMM36 force field was used.
The obtained results revealed that selected dehydrin can interact with both minor and major
DNA grooves. The phosphate groups from the DNA molecule form salt bridges with the
positively charged lysines from polylysine, K-segment, contributing to the complex stability.
Overall, we have provided evidence for possible dehydrin-DNA interactions. However, the
exact nature and significance of these interactions is still an area of active research in vitro.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD)",
pages = "57-57",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1997"
}

Senćanski, M., Prodić, I., Pantelić, A.,& Vidović, M.. (2023). Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD). in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 57-57.
https://hdl.handle.net/21.15107/rcub_imagine_1997

Senćanski M, Prodić I, Pantelić A, Vidović M. Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD). in 4th Belgrade Bioinformatics Conference. 2023;4:57-57.
https://hdl.handle.net/21.15107/rcub_imagine_1997 .

Senćanski, Milan, Prodić, Ivana, Pantelić, Ana, Vidović, Marija, "Dehydrins in the service of protecting the DNA helix from the aspect of molecular dynamics (MD)" in 4th Belgrade Bioinformatics Conference, 4 (2023):57-57,
https://hdl.handle.net/21.15107/rcub_imagine_1997 .

TY  - JOUR
AU  - Stojanović, Dejan
AU  - Galović, Vladislava
AU  - Terzin, Tomislav
AU  - Ačanski, Jelena
AU  - Vidović, Marija
AU  - Orlović, Saša
PY  - 2023
UR  - https://www.mdpi.com/2075-4450/14/5/455
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1851
AB  - The Heterogynidae are a small family of moths consisting of a single genus Heterogynis and sixteen described species distributed in the Mediterranean region. A species new to science, Heterogynis serbica sp. nov., is described from the locality of Srebrenac, Mt. Kopaonik, Republic of Serbia, Balkan Peninsula, by applying an integrative taxonomic approach using morpho-anatomical characteristics, wing morphometics and DNA barcoding. Male genitalia, scanning electron micrographs of adult male head anatomy, abdominal tergites/sternites, cocoons and habitats of the closely related species H. serbica sp. nov. and H. zikici are discussed and illustrated. Photographs of adult males and females, cocoons, plants in which the cocoons were found and habitats are shown. Importantly, marked differences in genital structure and other morphological characters were noted. These differences were confirmed with forewing morphometrics and COI-based DNA barcoding results. Additionally, DNA barcodes for H. serbica sp. nov. and H. zikici were compared against previously available data for the genus to evaluate the phylogenetic relationships. We conclude that deep, previously unknown and unexpected intrageneric morphological diversity exists in the genus Heterogynis.
T2  - Insects
T2  - Insects
T1  - The Genus Heterogynis Rambur, 1866 (Heterogynidae, Lepidoptera): Congruence of Molecular, Morphological and Morphometric Evidence Reveal New Species in Serbia
IS  - 5
SP  - 455
VL  - 14
DO  - 10.3390/insects14050455
ER  - 

@article{
author = "Stojanović, Dejan and Galović, Vladislava and Terzin, Tomislav and Ačanski, Jelena and Vidović, Marija and Orlović, Saša",
year = "2023",
abstract = "The Heterogynidae are a small family of moths consisting of a single genus Heterogynis and sixteen described species distributed in the Mediterranean region. A species new to science, Heterogynis serbica sp. nov., is described from the locality of Srebrenac, Mt. Kopaonik, Republic of Serbia, Balkan Peninsula, by applying an integrative taxonomic approach using morpho-anatomical characteristics, wing morphometics and DNA barcoding. Male genitalia, scanning electron micrographs of adult male head anatomy, abdominal tergites/sternites, cocoons and habitats of the closely related species H. serbica sp. nov. and H. zikici are discussed and illustrated. Photographs of adult males and females, cocoons, plants in which the cocoons were found and habitats are shown. Importantly, marked differences in genital structure and other morphological characters were noted. These differences were confirmed with forewing morphometrics and COI-based DNA barcoding results. Additionally, DNA barcodes for H. serbica sp. nov. and H. zikici were compared against previously available data for the genus to evaluate the phylogenetic relationships. We conclude that deep, previously unknown and unexpected intrageneric morphological diversity exists in the genus Heterogynis.",
journal = "Insects, Insects",
title = "The Genus Heterogynis Rambur, 1866 (Heterogynidae, Lepidoptera): Congruence of Molecular, Morphological and Morphometric Evidence Reveal New Species in Serbia",
number = "5",
pages = "455",
volume = "14",
doi = "10.3390/insects14050455"
}

Stojanović, D., Galović, V., Terzin, T., Ačanski, J., Vidović, M.,& Orlović, S.. (2023). The Genus Heterogynis Rambur, 1866 (Heterogynidae, Lepidoptera): Congruence of Molecular, Morphological and Morphometric Evidence Reveal New Species in Serbia. in Insects, 14(5), 455.
https://doi.org/10.3390/insects14050455

Stojanović D, Galović V, Terzin T, Ačanski J, Vidović M, Orlović S. The Genus Heterogynis Rambur, 1866 (Heterogynidae, Lepidoptera): Congruence of Molecular, Morphological and Morphometric Evidence Reveal New Species in Serbia. in Insects. 2023;14(5):455.
doi:10.3390/insects14050455 .

Stojanović, Dejan, Galović, Vladislava, Terzin, Tomislav, Ačanski, Jelena, Vidović, Marija, Orlović, Saša, "The Genus Heterogynis Rambur, 1866 (Heterogynidae, Lepidoptera): Congruence of Molecular, Morphological and Morphometric Evidence Reveal New Species in Serbia" in Insects, 14, no. 5 (2023):455,
https://doi.org/10.3390/insects14050455 . .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2023
UR  - https://thessinppo2023.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1850
AB  - Ramonda serbica Panc. is a resurrection plant that can survive long periods of desiccation and
fully restores its metabolic functions just one day after watering. The aim of this study was to
identify key candidates and metabolic pathways involved in R. serbica desiccation tolerance.
We combined differential transcriptomics and proteomics with the analysis of phenolics,
sugars, cell wall polymers and photosynthetic electron transport (PET) chain. TMT-based
proteomic analysis allowed the relative quantification of 1192 different protein groups, 408 of
which were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost
all differentially abundant proteins and transcripts related to photosynthetic processes were
downregulated in DL. Chlorophyll fluorescence measurements showed a shift from linear PET

to cyclic electron transport (CET). The levels of H2O2-scavenging enzymes, ascorbate-
glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase

(SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD
isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs;
mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation led to cell wall
remodelling related to GLP-derived H2O2/HO●

activity and pectin demethylesterification. This
comprehensive study contributes to understanding the role and regulation of important
metabolic pathways during desiccation with the final aim to help improving the drought
tolerance in crops.
PB  - International Plant Proteomics Organization (INPPO)
C3  - 5th International Conference on Plant Proteomics
T1  - Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1850
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2023",
abstract = "Ramonda serbica Panc. is a resurrection plant that can survive long periods of desiccation and
fully restores its metabolic functions just one day after watering. The aim of this study was to
identify key candidates and metabolic pathways involved in R. serbica desiccation tolerance.
We combined differential transcriptomics and proteomics with the analysis of phenolics,
sugars, cell wall polymers and photosynthetic electron transport (PET) chain. TMT-based
proteomic analysis allowed the relative quantification of 1192 different protein groups, 408 of
which were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost
all differentially abundant proteins and transcripts related to photosynthetic processes were
downregulated in DL. Chlorophyll fluorescence measurements showed a shift from linear PET

to cyclic electron transport (CET). The levels of H2O2-scavenging enzymes, ascorbate-
glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase

(SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD
isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs;
mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation led to cell wall
remodelling related to GLP-derived H2O2/HO●

activity and pectin demethylesterification. This
comprehensive study contributes to understanding the role and regulation of important
metabolic pathways during desiccation with the final aim to help improving the drought
tolerance in crops.",
publisher = "International Plant Proteomics Organization (INPPO)",
journal = "5th International Conference on Plant Proteomics",
title = "Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1850"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2023). Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.. in 5th International Conference on Plant Proteomics
International Plant Proteomics Organization (INPPO)..
https://hdl.handle.net/21.15107/rcub_imagine_1850

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.. in 5th International Conference on Plant Proteomics. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1850 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc." in 5th International Conference on Plant Proteomics (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1850 .

TY  - CONF
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Milić Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2008
AB  - Ramonda serbica Panc. is an ancient resurrection plant, that survives a long desiccation period
and fully recovers metabolic functions upon watering. The main characteristic of desiccationtolerant
plant species is their ability to accumulate protective late embryogenesis abounded
protein (LEAPs). To propose their role in R. serbica desiccation tolerance we structurally
analysed LEAPs in hydrated and desiccated leaves.
According to transcriptomics, 318 LEAPs were identified and classified into seven family
groups based on protein BLAST analysis and conserved motifs (Pfam). The largest LEAPs
belonged to the LEA2 and LEA4 protein family groups. We employed online tools to analyse
physicochemical characteristics (Expasy, ProtParam, BioPython, GRAVY calculator), disorder
propensity, and characterization protein structures (FELLS, JPred, SOPMA, PsiPred, Phyre2,
Espritz-DisProt, Espritz-X, Iupred, TMHMM, +Heliquest).
The most abundant, atypical LEA2 group containing 127, mostly hydrophobic proteins, was
divided into five subgroups. Members of this group were predicted to fold into globular
domains, β-barrel at the C-terminus, followed by transmembrane hydrophobic-helices and
disordered N-terminal regions. Results indicated the possible involvement in the protection
of the chloroplastic membranes.
The LEA4 group exhibited an exceptionally high tendency to form amphipathic α-helices and
simultaneously had a high disorder propensity. This group is made of 96 proteins, classified into 3
subgroups. The high content of polar and charged amino acids (lysine, glutamate, and aspartate) is
characteristic of this group. Motifs corresponding to the R. serbica LEA4 protein family group folded
into A-type α-helices that contained positive, negative, and hydrophobic surfaces. Based on previous
knowledge, the possible functions of the LEA2 and LEA4 groups are discussed with significant
implications on cell preservation technology and the improvement of crop drought tolerance.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.
EP  - 68
SP  - 68
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2008
ER  - 

@conference{
author = "Pantelić, Ana and Stevanović, Strahinja and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2023",
abstract = "Ramonda serbica Panc. is an ancient resurrection plant, that survives a long desiccation period
and fully recovers metabolic functions upon watering. The main characteristic of desiccationtolerant
plant species is their ability to accumulate protective late embryogenesis abounded
protein (LEAPs). To propose their role in R. serbica desiccation tolerance we structurally
analysed LEAPs in hydrated and desiccated leaves.
According to transcriptomics, 318 LEAPs were identified and classified into seven family
groups based on protein BLAST analysis and conserved motifs (Pfam). The largest LEAPs
belonged to the LEA2 and LEA4 protein family groups. We employed online tools to analyse
physicochemical characteristics (Expasy, ProtParam, BioPython, GRAVY calculator), disorder
propensity, and characterization protein structures (FELLS, JPred, SOPMA, PsiPred, Phyre2,
Espritz-DisProt, Espritz-X, Iupred, TMHMM, +Heliquest).
The most abundant, atypical LEA2 group containing 127, mostly hydrophobic proteins, was
divided into five subgroups. Members of this group were predicted to fold into globular
domains, β-barrel at the C-terminus, followed by transmembrane hydrophobic-helices and
disordered N-terminal regions. Results indicated the possible involvement in the protection
of the chloroplastic membranes.
The LEA4 group exhibited an exceptionally high tendency to form amphipathic α-helices and
simultaneously had a high disorder propensity. This group is made of 96 proteins, classified into 3
subgroups. The high content of polar and charged amino acids (lysine, glutamate, and aspartate) is
characteristic of this group. Motifs corresponding to the R. serbica LEA4 protein family group folded
into A-type α-helices that contained positive, negative, and hydrophobic surfaces. Based on previous
knowledge, the possible functions of the LEA2 and LEA4 groups are discussed with significant
implications on cell preservation technology and the improvement of crop drought tolerance.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.",
pages = "68-68",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2008"
}

Pantelić, A., Stevanović, S., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2023). Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 68-68.
https://hdl.handle.net/21.15107/rcub_imagine_2008

Pantelić A, Stevanović S, Milić Komić S, Kilibarda N, Vidović M. Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference. 2023;4:68-68.
https://hdl.handle.net/21.15107/rcub_imagine_2008 .

Pantelić, Ana, Stevanović, Strahinja, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc." in 4th Belgrade Bioinformatics Conference, 4 (2023):68-68,
https://hdl.handle.net/21.15107/rcub_imagine_2008 .

TY  - CONF
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Samardžić, Jelena
AU  - Banović Đeri, Bojana
AU  - Vidović, Marija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2035
AB  - Variegated Pelargonium zonale is a widely cultivated ornamental plant characterized by
green, photosynthetically active tissue (GL) and white, non-photosynthetic tissue (WL).
The aim of this study was to investigate the transcriptomic differences between these
two tissue types.
We performed RNA-seq analysis of GL and WL on Illumina HiSeq 2500 platform. The
raw reads were processed using in-house scripts to remove low-quality reads, adapter
sequences, poly-N sequences, and contaminants. High-quality clean reads were subjected
to de novo transcriptome assembly using Trinity (min_kmer_cov = 2, min_glue = 2). The
redundancy was removed and longest transcripts per cluster were selected as unigenes.
Gene expression levels were estimated using RSEM by mapping clean data back to the
assembled transcriptome (Bowtie2 with mismatch = 0). Differential expression analysis
between GL and WL (three biological replicates per each) was performed with DESeq2
R package (p values adjusted according to Benjamini and Hochberg for controlling False
Discovery Rate). Genes with abs (log2 FC) ≥ 2 and adjusted p value < 0.05 were assigned
as statistically significant differentially expressed. Functional enrichment analysis was
performed using GOseq R package and KOBAS software (corrected p < 0.05).
We annotated 85,374 unigenes (61.17%), providing a valuable resource for future
functional genomics studies. Out of 8896 gene clusters that were statistically significantly
differentially expressed between the green and white leaf tissues (p value < 0.05 and
abs(log2 fold change) ≥ 2), 5585 were upregulated in the WL, while 3311 were upregulated
in the GL. These findings shed light on the transcriptomic differences between the
two leaf tissue types in P. zonale and provide a foundation for further research on the
functional significance of these differences. Also, this study demonstrated utility of the
Trinity pipeline for de novo transcriptomic analysis of organism whose genomes are yet
not sequenced.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale
EP  - 90
SP  - 90
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2035
ER  - 

@conference{
author = "Milić, Dejana and Pantelić, Ana and Samardžić, Jelena and Banović Đeri, Bojana and Vidović, Marija",
year = "2023",
abstract = "Variegated Pelargonium zonale is a widely cultivated ornamental plant characterized by
green, photosynthetically active tissue (GL) and white, non-photosynthetic tissue (WL).
The aim of this study was to investigate the transcriptomic differences between these
two tissue types.
We performed RNA-seq analysis of GL and WL on Illumina HiSeq 2500 platform. The
raw reads were processed using in-house scripts to remove low-quality reads, adapter
sequences, poly-N sequences, and contaminants. High-quality clean reads were subjected
to de novo transcriptome assembly using Trinity (min_kmer_cov = 2, min_glue = 2). The
redundancy was removed and longest transcripts per cluster were selected as unigenes.
Gene expression levels were estimated using RSEM by mapping clean data back to the
assembled transcriptome (Bowtie2 with mismatch = 0). Differential expression analysis
between GL and WL (three biological replicates per each) was performed with DESeq2
R package (p values adjusted according to Benjamini and Hochberg for controlling False
Discovery Rate). Genes with abs (log2 FC) ≥ 2 and adjusted p value < 0.05 were assigned
as statistically significant differentially expressed. Functional enrichment analysis was
performed using GOseq R package and KOBAS software (corrected p < 0.05).
We annotated 85,374 unigenes (61.17%), providing a valuable resource for future
functional genomics studies. Out of 8896 gene clusters that were statistically significantly
differentially expressed between the green and white leaf tissues (p value < 0.05 and
abs(log2 fold change) ≥ 2), 5585 were upregulated in the WL, while 3311 were upregulated
in the GL. These findings shed light on the transcriptomic differences between the
two leaf tissue types in P. zonale and provide a foundation for further research on the
functional significance of these differences. Also, this study demonstrated utility of the
Trinity pipeline for de novo transcriptomic analysis of organism whose genomes are yet
not sequenced.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale",
pages = "90-90",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2035"
}

Milić, D., Pantelić, A., Samardžić, J., Banović Đeri, B.,& Vidović, M.. (2023). Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 90-90.
https://hdl.handle.net/21.15107/rcub_imagine_2035

Milić D, Pantelić A, Samardžić J, Banović Đeri B, Vidović M. Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale. in 4th Belgrade Bioinformatics Conference. 2023;4:90-90.
https://hdl.handle.net/21.15107/rcub_imagine_2035 .

Milić, Dejana, Pantelić, Ana, Samardžić, Jelena, Banović Đeri, Bojana, Vidović, Marija, "Efficient bioinformatics workflow for de novo transcriptome assembly of Pelargonium zonale" in 4th Belgrade Bioinformatics Conference, 4 (2023):90-90,
https://hdl.handle.net/21.15107/rcub_imagine_2035 .

TY  - CONF
AU  - Vidović, Marija
AU  - Galović, Vladislava
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2054
AB  - Insects are the most diverse group in the animal kingdom, accounting for about two-thirds
of all animals. Cytochrome c oxidase subunit 1 (COI) is the most commonly used marker
gene for animal species delineation. However, the accuracy of this approach crucially
depends on the degree of overlap between the intra- and interspecific variations.
Recently, we have identified a new species, Heterogynis serbica sp. n. (Lepidoptera:
Zygaenoidea, Heterogynidae) found on the Mt. Kopaonik, Republic of Serbia, Balkan
Peninsula. This was done by integrating taxonomic approach using morpho-anatomical
characteristics by comparative scanning electron microscopy (SEM), linear wing
morphometry and COI-based DNA barcoding [1]. In this study, we have used a set of
bioinformatics tools available online, to determine the differences in secondary and
tertiary structure of the COI proteins from H. serbica sp. n. and H. zikici. We also compared
the amino acid distribution and COI motif profiles between the two species. Our results
provide strong evidence that protein structure of COI can help with COI-based DNA
barcoding for taxon-specific purposes of species identification and delimitation studies.
Millions of COI DNA sequences deposited in the public domain (which are still growing)
carry huge potential for a comprehensive assessment of genetic variation in COI among
insects by using here described analysis.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation
EP  - 109
SP  - 109
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2054
ER  - 

@conference{
author = "Vidović, Marija and Galović, Vladislava",
year = "2023",
abstract = "Insects are the most diverse group in the animal kingdom, accounting for about two-thirds
of all animals. Cytochrome c oxidase subunit 1 (COI) is the most commonly used marker
gene for animal species delineation. However, the accuracy of this approach crucially
depends on the degree of overlap between the intra- and interspecific variations.
Recently, we have identified a new species, Heterogynis serbica sp. n. (Lepidoptera:
Zygaenoidea, Heterogynidae) found on the Mt. Kopaonik, Republic of Serbia, Balkan
Peninsula. This was done by integrating taxonomic approach using morpho-anatomical
characteristics by comparative scanning electron microscopy (SEM), linear wing
morphometry and COI-based DNA barcoding [1]. In this study, we have used a set of
bioinformatics tools available online, to determine the differences in secondary and
tertiary structure of the COI proteins from H. serbica sp. n. and H. zikici. We also compared
the amino acid distribution and COI motif profiles between the two species. Our results
provide strong evidence that protein structure of COI can help with COI-based DNA
barcoding for taxon-specific purposes of species identification and delimitation studies.
Millions of COI DNA sequences deposited in the public domain (which are still growing)
carry huge potential for a comprehensive assessment of genetic variation in COI among
insects by using here described analysis.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation",
pages = "109-109",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2054"
}

Vidović, M.,& Galović, V.. (2023). Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 109-109.
https://hdl.handle.net/21.15107/rcub_imagine_2054

Vidović M, Galović V. Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation. in 4th Belgrade Bioinformatics Conference. 2023;4:109-109.
https://hdl.handle.net/21.15107/rcub_imagine_2054 .

Vidović, Marija, Galović, Vladislava, "Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation" in 4th Belgrade Bioinformatics Conference, 4 (2023):109-109,
https://hdl.handle.net/21.15107/rcub_imagine_2054 .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Milić, Dejana
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2005
AB  - The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully
recovers metabolic functions within one day upon watering. We aimed to identify key
candidates and pathways involved in desiccation tolerance in R. serbica by employing a
systems biology approach, combining transcriptomics and proteomics.
A total of 68,694 differentially expressed genes (DEGs; p-value<0.005 and abs(log2FC)≥2) were
obtained in R. serbica leaves upon desiccation. Among them, 23,935 and 26,169 genes were
upregulated and downregulated in desiccated leaves (DL) and hydrated leaves (HL), respectively.
By differential TMT-based proteomic analysis 1192 different protein groups were identified
after filtering with at least two unique peptides per protein. In total, 229 protein groups
were more abundant in HL and 179 in DL (p-value<0.05 and abs(FC)≥1.3). The majority
of the DAPs and DEGs involved in photosynthesis, transport, secondary metabolism, and
signaling, were less abundant in DL. On the other hand, proteins and transcripts associated
with fermentation, N-metabolism, heme, protein synthesis, folding and assembly, C1-
metabolism, and late embryogenesis abundant proteins, were more accumulated in DL.
A poor correlation between proteomic and transcriptomic results was detected for mitochondrial
electron transport and ATP production, gluconeogenesis, glycolysis, tricarboxylic
acid cycle, and enzymatic H2O2 scavengers due to different mRNA half-life, protein turnover,
dynamic posttranscriptional and posttranslational modifications. Finally, desiccation tolerance
in R. serbica is a species-specific process orchestrated by several metabolic pathways
that are temporally and compartmentally regulated at several levels.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.
EP  - 65
SP  - 65
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2005
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Milić, Dejana and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2023",
abstract = "The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully
recovers metabolic functions within one day upon watering. We aimed to identify key
candidates and pathways involved in desiccation tolerance in R. serbica by employing a
systems biology approach, combining transcriptomics and proteomics.
A total of 68,694 differentially expressed genes (DEGs; p-value<0.005 and abs(log2FC)≥2) were
obtained in R. serbica leaves upon desiccation. Among them, 23,935 and 26,169 genes were
upregulated and downregulated in desiccated leaves (DL) and hydrated leaves (HL), respectively.
By differential TMT-based proteomic analysis 1192 different protein groups were identified
after filtering with at least two unique peptides per protein. In total, 229 protein groups
were more abundant in HL and 179 in DL (p-value<0.05 and abs(FC)≥1.3). The majority
of the DAPs and DEGs involved in photosynthesis, transport, secondary metabolism, and
signaling, were less abundant in DL. On the other hand, proteins and transcripts associated
with fermentation, N-metabolism, heme, protein synthesis, folding and assembly, C1-
metabolism, and late embryogenesis abundant proteins, were more accumulated in DL.
A poor correlation between proteomic and transcriptomic results was detected for mitochondrial
electron transport and ATP production, gluconeogenesis, glycolysis, tricarboxylic
acid cycle, and enzymatic H2O2 scavengers due to different mRNA half-life, protein turnover,
dynamic posttranscriptional and posttranslational modifications. Finally, desiccation tolerance
in R. serbica is a species-specific process orchestrated by several metabolic pathways
that are temporally and compartmentally regulated at several levels.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.",
pages = "65-65",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2005"
}

Vidović, M., Battisti, I., Pantelić, A., Milić, D., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2023). Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 65-65.
https://hdl.handle.net/21.15107/rcub_imagine_2005

Vidović M, Battisti I, Pantelić A, Milić D, Arrigoni G, Masi A, Veljović Jovanović S. Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference. 2023;4:65-65.
https://hdl.handle.net/21.15107/rcub_imagine_2005 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Milić, Dejana, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc." in 4th Belgrade Bioinformatics Conference, 4 (2023):65-65,
https://hdl.handle.net/21.15107/rcub_imagine_2005 .

TY  - JOUR
AU  - Milić, Dejana
AU  - Živanović, Bojana
AU  - Samardžić, Jelena
AU  - Nikolić, Nenad
AU  - Cukier, Caroline
AU  - Limami, Anis M.
AU  - Vidović, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1712
AB  - Plants are inevitably exposed to extreme climatic conditions that lead to a disturbed balance between the amount of absorbed energy and their ability to process it. Variegated leaves with photosynthetically active green leaf tissue (GL) and photosynthetically inactive white leaf tissue (WL) are an excellent model system to study source–sink interactions within the same leaf under the same microenvironmental conditions. We demonstrated that under excess excitation energy (EEE) conditions (high irradiance and lower temperature), regulated metabolic reprogramming in both leaf tissues allowed an increased consumption of reducing equivalents, as evidenced by preserved maximum efficiency of photosystem II (ФPSII) at the end of the experiment. GL of the EEE-treated plants employed two strategies: (i) the accumulation of flavonoid glycosides, especially cyanidin glycosides, as an alternative electron sink, and (ii) cell wall stiffening by cellulose, pectin, and lignin accumulation. On the other hand, WL increased the amount of free amino acids, mainly arginine, asparagine, branched-chain and aromatic amino acids, as well as kaempferol and quercetin glycosides. Thus, WL acts as an important energy escape valve that is required in order to maintain the successful performance of the GL sectors under EEE conditions. Finally, this role could be an adaptive value of variegation, as no consistent conclusions about its ecological benefits have been proposed so far.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions
IS  - 3
SP  - 2269
VL  - 24
DO  - 10.3390/ijms24032269
ER  - 

@article{
author = "Milić, Dejana and Živanović, Bojana and Samardžić, Jelena and Nikolić, Nenad and Cukier, Caroline and Limami, Anis M. and Vidović, Marija",
year = "2023",
abstract = "Plants are inevitably exposed to extreme climatic conditions that lead to a disturbed balance between the amount of absorbed energy and their ability to process it. Variegated leaves with photosynthetically active green leaf tissue (GL) and photosynthetically inactive white leaf tissue (WL) are an excellent model system to study source–sink interactions within the same leaf under the same microenvironmental conditions. We demonstrated that under excess excitation energy (EEE) conditions (high irradiance and lower temperature), regulated metabolic reprogramming in both leaf tissues allowed an increased consumption of reducing equivalents, as evidenced by preserved maximum efficiency of photosystem II (ФPSII) at the end of the experiment. GL of the EEE-treated plants employed two strategies: (i) the accumulation of flavonoid glycosides, especially cyanidin glycosides, as an alternative electron sink, and (ii) cell wall stiffening by cellulose, pectin, and lignin accumulation. On the other hand, WL increased the amount of free amino acids, mainly arginine, asparagine, branched-chain and aromatic amino acids, as well as kaempferol and quercetin glycosides. Thus, WL acts as an important energy escape valve that is required in order to maintain the successful performance of the GL sectors under EEE conditions. Finally, this role could be an adaptive value of variegation, as no consistent conclusions about its ecological benefits have been proposed so far.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions",
number = "3",
pages = "2269",
volume = "24",
doi = "10.3390/ijms24032269"
}

Milić, D., Živanović, B., Samardžić, J., Nikolić, N., Cukier, C., Limami, A. M.,& Vidović, M.. (2023). Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions. in International Journal of Molecular Sciences, 24(3), 2269.
https://doi.org/10.3390/ijms24032269

Milić D, Živanović B, Samardžić J, Nikolić N, Cukier C, Limami AM, Vidović M. Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions. in International Journal of Molecular Sciences. 2023;24(3):2269.
doi:10.3390/ijms24032269 .

Milić, Dejana, Živanović, Bojana, Samardžić, Jelena, Nikolić, Nenad, Cukier, Caroline, Limami, Anis M., Vidović, Marija, "Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions" in International Journal of Molecular Sciences, 24, no. 3 (2023):2269,
https://doi.org/10.3390/ijms24032269 . .

TY  - CONF
AU  - Pantelić, Ana
AU  - Senćanski, Milan
AU  - Prodić, Ivana
AU  - Milić, Dejana
AU  - Vidović, Marija
PY  - 2023
UR  - https://ml4ngp.eu/conference-bratislava/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1934
PB  - European Cooperation in Science and Technology
C3  - Non-globular proteins in the era of machine learning
T1  - Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation
EP  - 6
SP  - 5
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1934
ER  - 

@conference{
author = "Pantelić, Ana and Senćanski, Milan and Prodić, Ivana and Milić, Dejana and Vidović, Marija",
year = "2023",
publisher = "European Cooperation in Science and Technology",
journal = "Non-globular proteins in the era of machine learning",
title = "Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation",
pages = "6-5",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1934"
}

Pantelić, A., Senćanski, M., Prodić, I., Milić, D.,& Vidović, M.. (2023). Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation. in Non-globular proteins in the era of machine learning
European Cooperation in Science and Technology., 5-6.
https://hdl.handle.net/21.15107/rcub_imagine_1934

Pantelić A, Senćanski M, Prodić I, Milić D, Vidović M. Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation. in Non-globular proteins in the era of machine learning. 2023;:5-6.
https://hdl.handle.net/21.15107/rcub_imagine_1934 .

Pantelić, Ana, Senćanski, Milan, Prodić, Ivana, Milić, Dejana, Vidović, Marija, "Group 4 late embryogenesis abundant (LEA) proteins as a model to stydy propensity for oligomerisation" in Non-globular proteins in the era of machine learning (2023):5-6,
https://hdl.handle.net/21.15107/rcub_imagine_1934 .

TY  - CONF
AU  - Vidović, Marija
AU  - Pantelić, Ana
AU  - Senćanski, Milan
AU  - Prodić, Ivana
PY  - 2023
UR  - https://ml4ngp.eu/conference-bratislava/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1933
PB  - European Cooperation in Science and Technology
C3  - Non-globular proteins in the era of machine learning
T1  - LEA4 proteins: How disordered are they?
EP  - 50
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1933
ER  - 

@conference{
author = "Vidović, Marija and Pantelić, Ana and Senćanski, Milan and Prodić, Ivana",
year = "2023",
publisher = "European Cooperation in Science and Technology",
journal = "Non-globular proteins in the era of machine learning",
title = "LEA4 proteins: How disordered are they?",
pages = "50-49",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1933"
}

Vidović, M., Pantelić, A., Senćanski, M.,& Prodić, I.. (2023). LEA4 proteins: How disordered are they?. in Non-globular proteins in the era of machine learning
European Cooperation in Science and Technology., 49-50.
https://hdl.handle.net/21.15107/rcub_imagine_1933

Vidović M, Pantelić A, Senćanski M, Prodić I. LEA4 proteins: How disordered are they?. in Non-globular proteins in the era of machine learning. 2023;:49-50.
https://hdl.handle.net/21.15107/rcub_imagine_1933 .

Vidović, Marija, Pantelić, Ana, Senćanski, Milan, Prodić, Ivana, "LEA4 proteins: How disordered are they?" in Non-globular proteins in the era of machine learning (2023):49-50,
https://hdl.handle.net/21.15107/rcub_imagine_1933 .

TY  - JOUR
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Banović Đeri, Bojana
AU  - Samardžić, Jelena
AU  - Vidović, Marija
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/6/5288
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1795
AB  - The photosynthetically active green leaf (GL) and non-active white leaf (WL) tissues of variegated Pelargonium zonale provide an excellent model system for studying processes associated with photosynthesis and sink-source interactions, enabling the same microenvironmental conditions. By combining differential transcriptomics and metabolomics, we identified the main differences between these two metabolically contrasting tissues. Genes related to photosynthesis and associated pigments, the Calvin–Benson cycle, fermentation, and glycolysis were strongly repressed in WL. On the other hand, genes related to nitrogen and protein metabolism, defence, cytoskeletal components (motor proteins), cell division, DNA replication, repair and recombination, chromatin remodelling, and histone modifications were upregulated in WL. A content of soluble sugars, TCA intermediates, ascorbate, and hydroxybenzoic acids was lower, while the concentration of free amino acids (AAs), hydroxycinnamic acids, and several quercetin and kaempferol glycosides was higher in WL than in GL. Therefore, WL presents a carbon sink and depends on photosynthetic and energy-generating processes in GL. Furthermore, the upregulated nitrogen metabolism in WL compensates for the insufficient energy from carbon metabolism by providing alternative respiratory substrates. At the same time, WL serves as nitrogen storage. Overall, our study provides a new genetic data resource for the use of this excellent model system and for ornamental pelargonium breeding and contributes to uncovering molecular mechanisms underlying variegation and its adaptive ecological value.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study
IS  - 6
SP  - 5288
VL  - 24
DO  - 10.3390/ijms24065288
ER  - 

@article{
author = "Milić, Dejana and Pantelić, Ana and Banović Đeri, Bojana and Samardžić, Jelena and Vidović, Marija",
year = "2023",
abstract = "The photosynthetically active green leaf (GL) and non-active white leaf (WL) tissues of variegated Pelargonium zonale provide an excellent model system for studying processes associated with photosynthesis and sink-source interactions, enabling the same microenvironmental conditions. By combining differential transcriptomics and metabolomics, we identified the main differences between these two metabolically contrasting tissues. Genes related to photosynthesis and associated pigments, the Calvin–Benson cycle, fermentation, and glycolysis were strongly repressed in WL. On the other hand, genes related to nitrogen and protein metabolism, defence, cytoskeletal components (motor proteins), cell division, DNA replication, repair and recombination, chromatin remodelling, and histone modifications were upregulated in WL. A content of soluble sugars, TCA intermediates, ascorbate, and hydroxybenzoic acids was lower, while the concentration of free amino acids (AAs), hydroxycinnamic acids, and several quercetin and kaempferol glycosides was higher in WL than in GL. Therefore, WL presents a carbon sink and depends on photosynthetic and energy-generating processes in GL. Furthermore, the upregulated nitrogen metabolism in WL compensates for the insufficient energy from carbon metabolism by providing alternative respiratory substrates. At the same time, WL serves as nitrogen storage. Overall, our study provides a new genetic data resource for the use of this excellent model system and for ornamental pelargonium breeding and contributes to uncovering molecular mechanisms underlying variegation and its adaptive ecological value.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study",
number = "6",
pages = "5288",
volume = "24",
doi = "10.3390/ijms24065288"
}

Milić, D., Pantelić, A., Banović Đeri, B., Samardžić, J.,& Vidović, M.. (2023). Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study. in International Journal of Molecular Sciences, 24(6), 5288.
https://doi.org/10.3390/ijms24065288

Milić D, Pantelić A, Banović Đeri B, Samardžić J, Vidović M. Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study. in International Journal of Molecular Sciences. 2023;24(6):5288.
doi:10.3390/ijms24065288 .

Milić, Dejana, Pantelić, Ana, Banović Đeri, Bojana, Samardžić, Jelena, Vidović, Marija, "Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study" in International Journal of Molecular Sciences, 24, no. 6 (2023):5288,
https://doi.org/10.3390/ijms24065288 . .

TY  - CONF
AU  - Golob, Aleksandra
AU  - Ojdanič, Nik
AU  - Živanović, Bojana
AU  - Germ, Mateja
AU  - Milić Komić, Sonja
AU  - Sedlarević Zorić, Ana
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Mavrič Čermelj, Anja
AU  - Samardžić, Jelena
AU  - Veljovic Jovanovic, Sonja
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1839
AB  - Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered abeneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperaturesby promoting antioxidant production. However, the underlying mechanisms are still notelucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed towater shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impactsphotochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflectiveand transmissive properties. Si application between flag leaf emergence and grain fillinghad the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was themost abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was theonly hydroxycinnamic acid showing significant accumulation with the latest applied Si comparedto no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to droughtwithout Si addition, while lysine was the most accumulated in the leaves supplemented by Si atthe grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to droughtregardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, itseffect was dependent on the barley growth phase prior to supplementation.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting
T1  - The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought
SP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1839
ER  - 

@conference{
author = "Golob, Aleksandra and Ojdanič, Nik and Živanović, Bojana and Germ, Mateja and Milić Komić, Sonja and Sedlarević Zorić, Ana and Milić, Dejana and Pantelić, Ana and Mavrič Čermelj, Anja and Samardžić, Jelena and Veljovic Jovanovic, Sonja and Vidović, Marija",
year = "2022",
abstract = "Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered abeneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperaturesby promoting antioxidant production. However, the underlying mechanisms are still notelucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed towater shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impactsphotochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflectiveand transmissive properties. Si application between flag leaf emergence and grain fillinghad the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was themost abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was theonly hydroxycinnamic acid showing significant accumulation with the latest applied Si comparedto no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to droughtwithout Si addition, while lysine was the most accumulated in the leaves supplemented by Si atthe grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to droughtregardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, itseffect was dependent on the barley growth phase prior to supplementation.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting",
title = "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought",
pages = "67",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1839"
}

Golob, A., Ojdanič, N., Živanović, B., Germ, M., Milić Komić, S., Sedlarević Zorić, A., Milić, D., Pantelić, A., Mavrič Čermelj, A., Samardžić, J., Veljovic Jovanovic, S.,& Vidović, M.. (2022). The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in 4th International Conference on Plant Biology and 23rd SPPS Meeting
Serbian Plant Physiology Society., 67.
https://hdl.handle.net/21.15107/rcub_imagine_1839

Golob A, Ojdanič N, Živanović B, Germ M, Milić Komić S, Sedlarević Zorić A, Milić D, Pantelić A, Mavrič Čermelj A, Samardžić J, Veljovic Jovanovic S, Vidović M. The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:67.
https://hdl.handle.net/21.15107/rcub_imagine_1839 .

Golob, Aleksandra, Ojdanič, Nik, Živanović, Bojana, Germ, Mateja, Milić Komić, Sonja, Sedlarević Zorić, Ana, Milić, Dejana, Pantelić, Ana, Mavrič Čermelj, Anja, Samardžić, Jelena, Veljovic Jovanovic, Sonja, Vidović, Marija, "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):67,
https://hdl.handle.net/21.15107/rcub_imagine_1839 .

TY  - CONF
AU  - Vidović, Marija
AU  - Tosti, T.
AU  - Nikolić, N.
AU  - Pantelić, Ana
AU  - Veljović Jovanović, S.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1868
UR  - http://www.sfses.com/docs/14th-SFSES-Abstracts.pdf
AB  - Resurrection plant Ramonda serbica Panc. survives long desiccation periods
and fully recovers metabolic functions already one day upon watering. This study
aimed to investigate the role of soluble sugars and polyphenols, as well as cell wallassociated
polysaccharides and lignin in desiccation tolerance in R. serbica, an
endemic species from the Balkan peninsula. We combined differential
transcriptomics and proteomics, the analysis of soluble polyphenolics and sugars, as
well as FTIR analysis of the cell wall polymers. Pectin, cellulose, hemicellulose, and
xyloglucans were identified as polysaccharide components of the R. serbica cell
wall and they decreased upon desiccation. Desiccation provoked cell wall
remodelling related to the possible production of H2O2/HO via germin-like proteins
and pectin demethylesterification. In addition, desiccation induced carbon recycling
from starch to soluble sugar osmolytes, whose content significantly increased in
desiccated leaves. These data support the importance of specific sugars and the
plasticity of the cell wall as one of the major contributors to desiccation tolerance of
resurrection species, contributing to further crop drought tolerance improvement.
PB  - Belgrade : Institute for Nature Conservation of Serbia
PB  - Niš : Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions
T1  - The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1868
ER  - 

@conference{
author = "Vidović, Marija and Tosti, T. and Nikolić, N. and Pantelić, Ana and Veljović Jovanović, S.",
year = "2022",
abstract = "Resurrection plant Ramonda serbica Panc. survives long desiccation periods
and fully recovers metabolic functions already one day upon watering. This study
aimed to investigate the role of soluble sugars and polyphenols, as well as cell wallassociated
polysaccharides and lignin in desiccation tolerance in R. serbica, an
endemic species from the Balkan peninsula. We combined differential
transcriptomics and proteomics, the analysis of soluble polyphenolics and sugars, as
well as FTIR analysis of the cell wall polymers. Pectin, cellulose, hemicellulose, and
xyloglucans were identified as polysaccharide components of the R. serbica cell
wall and they decreased upon desiccation. Desiccation provoked cell wall
remodelling related to the possible production of H2O2/HO via germin-like proteins
and pectin demethylesterification. In addition, desiccation induced carbon recycling
from starch to soluble sugar osmolytes, whose content significantly increased in
desiccated leaves. These data support the importance of specific sugars and the
plasticity of the cell wall as one of the major contributors to desiccation tolerance of
resurrection species, contributing to further crop drought tolerance improvement.",
publisher = "Belgrade : Institute for Nature Conservation of Serbia, Niš : Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions",
title = "The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1868"
}

Vidović, M., Tosti, T., Nikolić, N., Pantelić, A.,& Veljović Jovanović, S.. (2022). The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions
Belgrade : Institute for Nature Conservation of Serbia., 73.
https://hdl.handle.net/21.15107/rcub_imagine_1868

Vidović M, Tosti T, Nikolić N, Pantelić A, Veljović Jovanović S. The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions. 2022;:73.
https://hdl.handle.net/21.15107/rcub_imagine_1868 .

Vidović, Marija, Tosti, T., Nikolić, N., Pantelić, Ana, Veljović Jovanović, S., "The role of polyphenols, sugars, and cell-wall associated polymers in desiccation tolerance of Ramonda serbica" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions (2022):73,
https://hdl.handle.net/21.15107/rcub_imagine_1868 .

TY  - CONF
AU  - Savić, Aleksa
AU  - Vidović, Marija
AU  - Radosavljević, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1854
AB  - Fluorescentno obeleženi proteini su neprocenjivi alati u laboratorijskoj praksi za in vivo lokalizovanje i ispitivanje interakcija proteina. Dizajnirali smo vektor za ekspresiju mCerulean3 sa N-terminalnim heksahistidinskim obeleživacem fuzionisanim preko poliasparaginskog linkera i proteolitickog mesta za proteazu virusa graviranosti duvana (TEV) sa -sinukleinom. Ovaj konstrukt može se upotrebiti za proizvodnju -sinukleina nativne sekvence nakon proteolize TEV proteazom. Gen za mCerulean3 je nizom lancanih reakcija polimeraze (SOEing PCR) fuzionisan sa genom za -sinuklein i nakon amplifikacije ukloniran u plazmid pDUET-1. Escherichia coli BL21(DE3) je, nakon transformacije ovim konstruktom, upotrebljena za proizvodnju himernog proteina koji je zadržao fluorescentna svojstva sa prinosom od ~2 mg po litru medijuma nakon precišcavanja imobilizovanom metal-afinitetnom hromatografijom (elektroforetska cistoca: ~80%). Ovaj himerni protein je uspešno proteolizovan TEV proteazom.
AB  - Fluorescently labeled proteins are invaluable tools in laboratory practice to assess the in vivo localization and the interactions of proteins. Here we have designed an expression vector with an N-terminal hexahistidine-tagged mCerulean3 fused through a polyasparagine linker and the proteolytic site of tobacco etch virus protease (TEV) to - synuclein. This construct can be used to produce -synuclein of a native sequence after proteolysis with TEV protease. After fusion of the genes for mCerulean3 and -synuclein through a series of polymerase chain reactions (SOEing PCR), the resulting gene for the chimeric protein was cloned into the pDUET-1 plasmid. Escherichia coli BL21(DE3), upon transformation with this construct, can be used to produce the chimeric protein that retained the fluorescent properties of mCerulean3, with a yield of ~2 mg per liter of medium after purification by immobilized metal-affinity chromatography (electrophoretic purity: ~80%). This chimeric protein was successfully proteolyzed by TEV protease.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
T1  - Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli
T1  - Cloning and expression of fluorescently labeled )-synuclein in Eschierichia coli
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1854
ER  - 

@conference{
author = "Savić, Aleksa and Vidović, Marija and Radosavljević, Jelena",
year = "2022",
abstract = "Fluorescentno obeleženi proteini su neprocenjivi alati u laboratorijskoj praksi za in vivo lokalizovanje i ispitivanje interakcija proteina. Dizajnirali smo vektor za ekspresiju mCerulean3 sa N-terminalnim heksahistidinskim obeleživacem fuzionisanim preko poliasparaginskog linkera i proteolitickog mesta za proteazu virusa graviranosti duvana (TEV) sa -sinukleinom. Ovaj konstrukt može se upotrebiti za proizvodnju -sinukleina nativne sekvence nakon proteolize TEV proteazom. Gen za mCerulean3 je nizom lancanih reakcija polimeraze (SOEing PCR) fuzionisan sa genom za -sinuklein i nakon amplifikacije ukloniran u plazmid pDUET-1. Escherichia coli BL21(DE3) je, nakon transformacije ovim konstruktom, upotrebljena za proizvodnju himernog proteina koji je zadržao fluorescentna svojstva sa prinosom od ~2 mg po litru medijuma nakon precišcavanja imobilizovanom metal-afinitetnom hromatografijom (elektroforetska cistoca: ~80%). Ovaj himerni protein je uspešno proteolizovan TEV proteazom., Fluorescently labeled proteins are invaluable tools in laboratory practice to assess the in vivo localization and the interactions of proteins. Here we have designed an expression vector with an N-terminal hexahistidine-tagged mCerulean3 fused through a polyasparagine linker and the proteolytic site of tobacco etch virus protease (TEV) to - synuclein. This construct can be used to produce -synuclein of a native sequence after proteolysis with TEV protease. After fusion of the genes for mCerulean3 and -synuclein through a series of polymerase chain reactions (SOEing PCR), the resulting gene for the chimeric protein was cloned into the pDUET-1 plasmid. Escherichia coli BL21(DE3), upon transformation with this construct, can be used to produce the chimeric protein that retained the fluorescent properties of mCerulean3, with a yield of ~2 mg per liter of medium after purification by immobilized metal-affinity chromatography (electrophoretic purity: ~80%). This chimeric protein was successfully proteolyzed by TEV protease.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine",
title = "Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli, Cloning and expression of fluorescently labeled )-synuclein in Eschierichia coli",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1854"
}

Savić, A., Vidović, M.,& Radosavljević, J.. (2022). Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli. in 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
Beograd : Srpsko hemijsko društvo..
https://hdl.handle.net/21.15107/rcub_imagine_1854

Savić A, Vidović M, Radosavljević J. Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli. in 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine. 2022;.
https://hdl.handle.net/21.15107/rcub_imagine_1854 .

Savić, Aleksa, Vidović, Marija, Radosavljević, Jelena, "Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli" in 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine (2022),
https://hdl.handle.net/21.15107/rcub_imagine_1854 .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Veljović Jovanović, Sonja
AU  - Pantelić, Ana
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1867
AB  - Ressurection plants are extraordinary because of their ability to withstand long periods
without water, enter a state of anhydrobiosis, and fully recover upon water arrival.
Ramonda serbica is a relic and endemic species that belong to a very small group of
desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental
conditions have been an appealing subject to many researchers. Most of the genes
responsible for this amazing ability are present in other plants, and this path of research
where those genes could be activated in crops is growing much more attention because of
the imminent crisis regarding food supplies in the near future. Key components involved in
the response to dehydration in R. serbica plants were analysed through a comprehensive
transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis
abundant proteins play a significant role in the complex defence processes involved in
desiccation tolerance. Defining physicochemical characteristics and specific physiological
functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability
in other areas of research.
PB  - Belgrade : Faculty of Chemistry, Serbian Biochemical Societ
C3  - Amazing Biochemistry
T1  - Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein
SP  - 37
VL  - 11
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1867
ER  - 

@conference{
author = "Milić Komić, Sonja and Veljović Jovanović, Sonja and Pantelić, Ana and Vidović, Marija",
year = "2022",
abstract = "Ressurection plants are extraordinary because of their ability to withstand long periods
without water, enter a state of anhydrobiosis, and fully recover upon water arrival.
Ramonda serbica is a relic and endemic species that belong to a very small group of
desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental
conditions have been an appealing subject to many researchers. Most of the genes
responsible for this amazing ability are present in other plants, and this path of research
where those genes could be activated in crops is growing much more attention because of
the imminent crisis regarding food supplies in the near future. Key components involved in
the response to dehydration in R. serbica plants were analysed through a comprehensive
transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis
abundant proteins play a significant role in the complex defence processes involved in
desiccation tolerance. Defining physicochemical characteristics and specific physiological
functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability
in other areas of research.",
publisher = "Belgrade : Faculty of Chemistry, Serbian Biochemical Societ",
journal = "Amazing Biochemistry",
title = "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein",
pages = "37",
volume = "11",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1867"
}

Milić Komić, S., Veljović Jovanović, S., Pantelić, A.,& Vidović, M.. (2022). Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Amazing Biochemistry
Belgrade : Faculty of Chemistry, Serbian Biochemical Societ., 11, 37.
https://hdl.handle.net/21.15107/rcub_imagine_1867

Milić Komić S, Veljović Jovanović S, Pantelić A, Vidović M. Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Amazing Biochemistry. 2022;11:37.
https://hdl.handle.net/21.15107/rcub_imagine_1867 .

Milić Komić, Sonja, Veljović Jovanović, Sonja, Pantelić, Ana, Vidović, Marija, "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein" in Amazing Biochemistry, 11 (2022):37,
https://hdl.handle.net/21.15107/rcub_imagine_1867 .

TY  - CONF
AU  - Nikolić, Ljiljana
AU  - Vidović, Marija
AU  - Todorović, Nataša
AU  - Petković, Branka
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Bogdanović Pristov, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2168
AB  - Investigating membrane properties of plants is a challenging task, considering that success ofexperiments is highly dependent on the possibility to isolate metabolically active protoplasts thatcan withstand membrane current recordings. The aim of the present work is to obtain viableprotoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplastswith preserved membrane integrity suitable for electrophysiological experiments. We applied acustom approach for patch-clamping protoplasts using a microscope with a movable microscopestage. We recorded prominent inward and prominent outward types of membrane current profiles ofprotoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia
T1  - Protoplast patch-clamping using an upright microscope with a movable stage
EP  - 274
SP  - 271
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5508
ER  - 

@conference{
editor = "Čupić, Željko, Anić, Slobodan",
author = "Nikolić, Ljiljana and Vidović, Marija and Todorović, Nataša and Petković, Branka and Stojadinović, Gordana and Martać, Ljiljana and Bogdanović Pristov, Jelena",
year = "2022",
abstract = "Investigating membrane properties of plants is a challenging task, considering that success ofexperiments is highly dependent on the possibility to isolate metabolically active protoplasts thatcan withstand membrane current recordings. The aim of the present work is to obtain viableprotoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplastswith preserved membrane integrity suitable for electrophysiological experiments. We applied acustom approach for patch-clamping protoplasts using a microscope with a movable microscopestage. We recorded prominent inward and prominent outward types of membrane current profiles ofprotoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia",
title = "Protoplast patch-clamping using an upright microscope with a movable stage",
pages = "274-271",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5508"
}

Čupić, Ž., Anić, S., Nikolić, L., Vidović, M., Todorović, N., Petković, B., Stojadinović, G., Martać, L.,& Bogdanović Pristov, J.. (2022). Protoplast patch-clamping using an upright microscope with a movable stage. in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia
Belgrade : Society of Physical Chemists of Serbia., 271-274.
https://hdl.handle.net/21.15107/rcub_ibiss_5508

Čupić Ž, Anić S, Nikolić L, Vidović M, Todorović N, Petković B, Stojadinović G, Martać L, Bogdanović Pristov J. Protoplast patch-clamping using an upright microscope with a movable stage. in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia. 2022;:271-274.
https://hdl.handle.net/21.15107/rcub_ibiss_5508 .

Čupić, Željko, Anić, Slobodan, Nikolić, Ljiljana, Vidović, Marija, Todorović, Nataša, Petković, Branka, Stojadinović, Gordana, Martać, Ljiljana, Bogdanović Pristov, Jelena, "Protoplast patch-clamping using an upright microscope with a movable stage" in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia (2022):271-274,
https://hdl.handle.net/21.15107/rcub_ibiss_5508 .

TY  - CONF
AU  - Savić, Aleksa D.
AU  - Vidović, Marija
AU  - Radosavljević, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1832
AB  - Fluorescentno obeleženi proteini su neprocenjivi alati u laboratorijskoj praksi za in vivo lokalizovanje i ispitivanje interakcija proteina. Dizajnirali smo vektor za ekspresiju mCerulean3 sa N-terminalnim heksahistidinskim obeleživacem fuzionisanim preko poliasparaginskog linkera i proteolitickog mesta za proteazu virusa graviranosti duvana (TEV) sa -sinukleinom. Ovaj konstrukt može se upotrebiti za proizvodnju -sinukleina nativne sekvence nakon proteolize TEV proteazom. Gen za mCerulean3 je nizom lancanih reakcija polimeraze (SOEing PCR) fuzionisan sa genom za -sinuklein i nakon amplifikacije ukloniran u plazmid pDUET-1. Escherichia coli BL21(DE3) je, nakon transformacije ovim konstruktom, upotrebljena za proizvodnju himernog proteina koji je zadržao fluorescentna svojstva sa prinosom od ~2 mg po litru medijuma nakon precišcavanja imobilizovanom metal-afinitetnom hromatografijom (elektroforetska cistoca: ~80%). Ovaj himerni protein je uspešno proteolizovan TEV proteazom.
AB  - Fluorescently labeled proteins are invaluable tools in laboratory practice to assess the in vivo localization and the interactions of proteins. Here we have designed an expression vector with an N-terminal hexahistidine-tagged mCerulean3 fused through a polyasparagine linker and the proteolytic site of tobacco etch virus protease (TEV) to - synuclein. This construct can be used to produce -synuclein of a native sequence after proteolysis with TEV protease. After fusion of the genes for mCerulean3 and -synuclein through a series of polymerase chain reactions (SOEing PCR), the resulting gene for the chimeric protein was cloned into the pDUET-1 plasmid. Escherichia coli BL21(DE3), upon transformation with this construct, can be used to produce the chimeric protein that retained the fluorescent properties of mCerulean3, with a yield of ~2 mg per liter of medium after purification by immobilized metal-affinity chromatography (electrophoretic purity: ~80%). This chimeric protein was successfully proteolyzed by TEV protease.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine
T1  - Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli
T1  - Cloning and expression of fluorescently labeled )-synuclein in Eschierichia coli
EP  - 68
SP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1832
ER  - 

@conference{
author = "Savić, Aleksa D. and Vidović, Marija and Radosavljević, Jelena",
year = "2022",
abstract = "Fluorescentno obeleženi proteini su neprocenjivi alati u laboratorijskoj praksi za in vivo lokalizovanje i ispitivanje interakcija proteina. Dizajnirali smo vektor za ekspresiju mCerulean3 sa N-terminalnim heksahistidinskim obeleživacem fuzionisanim preko poliasparaginskog linkera i proteolitickog mesta za proteazu virusa graviranosti duvana (TEV) sa -sinukleinom. Ovaj konstrukt može se upotrebiti za proizvodnju -sinukleina nativne sekvence nakon proteolize TEV proteazom. Gen za mCerulean3 je nizom lancanih reakcija polimeraze (SOEing PCR) fuzionisan sa genom za -sinuklein i nakon amplifikacije ukloniran u plazmid pDUET-1. Escherichia coli BL21(DE3) je, nakon transformacije ovim konstruktom, upotrebljena za proizvodnju himernog proteina koji je zadržao fluorescentna svojstva sa prinosom od ~2 mg po litru medijuma nakon precišcavanja imobilizovanom metal-afinitetnom hromatografijom (elektroforetska cistoca: ~80%). Ovaj himerni protein je uspešno proteolizovan TEV proteazom., Fluorescently labeled proteins are invaluable tools in laboratory practice to assess the in vivo localization and the interactions of proteins. Here we have designed an expression vector with an N-terminal hexahistidine-tagged mCerulean3 fused through a polyasparagine linker and the proteolytic site of tobacco etch virus protease (TEV) to - synuclein. This construct can be used to produce -synuclein of a native sequence after proteolysis with TEV protease. After fusion of the genes for mCerulean3 and -synuclein through a series of polymerase chain reactions (SOEing PCR), the resulting gene for the chimeric protein was cloned into the pDUET-1 plasmid. Escherichia coli BL21(DE3), upon transformation with this construct, can be used to produce the chimeric protein that retained the fluorescent properties of mCerulean3, with a yield of ~2 mg per liter of medium after purification by immobilized metal-affinity chromatography (electrophoretic purity: ~80%). This chimeric protein was successfully proteolyzed by TEV protease.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine",
title = "Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli, Cloning and expression of fluorescently labeled )-synuclein in Eschierichia coli",
pages = "68-68",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1832"
}

Savić, A. D., Vidović, M.,& Radosavljević, J.. (2022). Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli. in 58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine
Beograd : Srpsko hemijsko društvo., 68-68.
https://hdl.handle.net/21.15107/rcub_imagine_1832

Savić AD, Vidović M, Radosavljević J. Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli. in 58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine. 2022;:68-68.
https://hdl.handle.net/21.15107/rcub_imagine_1832 .

Savić, Aleksa D., Vidović, Marija, Radosavljević, Jelena, "Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli" in 58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine (2022):68-68,
https://hdl.handle.net/21.15107/rcub_imagine_1832 .

TY  - CONF
AU  - Živanović, Bojana
AU  - Prokić, Ljiljana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1842
AB  - The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1842
ER  - 

@conference{
author = "Živanović, Bojana and Prokić, Ljiljana and Milić Komić, Sonja and Nikolić, Nenad and Sedlarević Zorić, Ana and Vidović, Marija and Veljović Jovanović, Sonja",
year = "2022",
abstract = "The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1842"
}

Živanović, B., Prokić, L., Milić Komić, S., Nikolić, N., Sedlarević Zorić, A., Vidović, M.,& Veljović Jovanović, S.. (2022). Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 83.
https://hdl.handle.net/21.15107/rcub_imagine_1842

Živanović B, Prokić L, Milić Komić S, Nikolić N, Sedlarević Zorić A, Vidović M, Veljović Jovanović S. Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;:83.
https://hdl.handle.net/21.15107/rcub_imagine_1842 .

Živanović, Bojana, Prokić, Ljiljana, Milić Komić, Sonja, Nikolić, Nenad, Sedlarević Zorić, Ana, Vidović, Marija, Veljović Jovanović, Sonja, "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca" in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022):83,
https://hdl.handle.net/21.15107/rcub_imagine_1842 .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1847
AB  - Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study was to identify key candidates and pathways involved in desiccation
tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,
proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the
cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain
were performed. In total, 1192 different protein groups were quantified by TMT-based comparative
quantitative proteomics. Among them, 408 protein groups showed a statistically significant
difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the
majority of proteins related to photosynthetic processes were less abundant, while chlorophyll
fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic
electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione
cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were
reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins
(GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),
were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall
polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/
HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific
process orchestrated by several metabolic pathways and regulatory networks acting at the transcript,
protein, metabolite and physiological levels.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting
T1  - Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1847
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2022",
abstract = "Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study was to identify key candidates and pathways involved in desiccation
tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,
proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the
cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain
were performed. In total, 1192 different protein groups were quantified by TMT-based comparative
quantitative proteomics. Among them, 408 protein groups showed a statistically significant
difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the
majority of proteins related to photosynthetic processes were less abundant, while chlorophyll
fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic
electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione
cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were
reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins
(GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),
were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall
polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/
HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific
process orchestrated by several metabolic pathways and regulatory networks acting at the transcript,
protein, metabolite and physiological levels.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting",
title = "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1847"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2022). Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 57.
https://hdl.handle.net/21.15107/rcub_imagine_1847

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:57.
https://hdl.handle.net/21.15107/rcub_imagine_1847 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):57,
https://hdl.handle.net/21.15107/rcub_imagine_1847 .

TY  - CONF
AU  - Savić, Aleksa
AU  - Vidović, Marija
AU  - Radosavljević, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1831
AB  - Alpha-synuclein is an intrinsically disordered protein prone to aggregation and it is involved in the formation of brain tissue amyloids in patients with Parkinson's and other neurodegenerative diseases. By fluorescently labeling recombinantly expressed proteins, the processes of expression and purification of the same can be easily visually monitored. In a previous work, we have constructed three plasmids for the expression of α-synuclein fused C-terminally to His-tagged mCerulean3 through a polyasparagine linker and tobacco etch virus (TEV) proteolytic site. Here we have transformed these vectors into Escherichia coli BL21(DE3) and BL21(DE3)pLysS and optimized the expression conditions by response surface methodology (temperature, time after induction, concentration of induction reagent) of the chimeric proteins coded by vectors. Expression of the chimeric protein was tested at optimal conditions in different media and terrific broth gave the highest yield. The obtained chimeric protein was purified by immobilized metal-affinity chromatography (IMAC), yielding ~29 mg of chimeric protein per liter of medium and was shown to be successfully proteolyzed by TEV protease, giving a fragment of α-synuclein of expected electrophoretic mobility. After another round of IMAC, the obtained α-synuclein of native sequence was mainly found to be in the flow-through.
PB  - Serbian Chemical Society
PB  - Serbian Young Chemists’ Club
C3  - 8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade
T1  - Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease
EP  - 10
SP  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1831
ER  - 

@conference{
author = "Savić, Aleksa and Vidović, Marija and Radosavljević, Jelena",
year = "2022",
abstract = "Alpha-synuclein is an intrinsically disordered protein prone to aggregation and it is involved in the formation of brain tissue amyloids in patients with Parkinson's and other neurodegenerative diseases. By fluorescently labeling recombinantly expressed proteins, the processes of expression and purification of the same can be easily visually monitored. In a previous work, we have constructed three plasmids for the expression of α-synuclein fused C-terminally to His-tagged mCerulean3 through a polyasparagine linker and tobacco etch virus (TEV) proteolytic site. Here we have transformed these vectors into Escherichia coli BL21(DE3) and BL21(DE3)pLysS and optimized the expression conditions by response surface methodology (temperature, time after induction, concentration of induction reagent) of the chimeric proteins coded by vectors. Expression of the chimeric protein was tested at optimal conditions in different media and terrific broth gave the highest yield. The obtained chimeric protein was purified by immobilized metal-affinity chromatography (IMAC), yielding ~29 mg of chimeric protein per liter of medium and was shown to be successfully proteolyzed by TEV protease, giving a fragment of α-synuclein of expected electrophoretic mobility. After another round of IMAC, the obtained α-synuclein of native sequence was mainly found to be in the flow-through.",
publisher = "Serbian Chemical Society, Serbian Young Chemists’ Club",
journal = "8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade",
title = "Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease",
pages = "10-10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1831"
}

Savić, A., Vidović, M.,& Radosavljević, J.. (2022). Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease. in 8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade
Serbian Chemical Society., 10-10.
https://hdl.handle.net/21.15107/rcub_imagine_1831

Savić A, Vidović M, Radosavljević J. Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease. in 8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade. 2022;:10-10.
https://hdl.handle.net/21.15107/rcub_imagine_1831 .

Savić, Aleksa, Vidović, Marija, Radosavljević, Jelena, "Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease" in 8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade (2022):10-10,
https://hdl.handle.net/21.15107/rcub_imagine_1831 .

TY  - CONF
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Banović Đeri, Bojana
AU  - Samardžić, Jelena
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1848
AB  - In the global warming era, we are facing extreme changes in environmental conditions such
as high and low temperatures, high light and ultraviolet radiation intensity, as well as drought
and increased CO2 levels. The effects of these factors on plants are often interrelated and usually
result in a disturbed balance between the amount of energy received and the ability to process
it. In order to balance energy input and prevent photooxidative damage, plants have evolved
multiple mechanisms for energy dissipation and photoprotection. To efficiently dissipate the excess
excitation energy (EEE), the additional electron sinks, such as photorespiration, biosynthesis
of phenolics and nitrate reduction are stimulated. Variegated leaves of Pelargonium zonale have
been proven to be a suitable model system for examining ‘source–sink’ interactions within the
same leaf, providing the same microenvironment conditions, unlike common root-shoot studies.
The aim of our study was to investigate carbon and nitrogen reallocation between photosynthetically
active (green leaf sectors) and photosynthetically inactive (white leaf sectors) under EEE
pressure induced by low temperature in combination with high light intensity. Besides the amino
acids and phenolics analyses, we monitored morphological and photosynthetic parameters of
P. zonale leaves. Our results revealed higher content of free proteogenic amino acids in the white
leaf sectors than in the green ones. Moreover, EEE triggered increased polyphenol synthesis, namely
anthocyanins, already on the fourth day of the experiment. Also, the leaf fresh/dry weight (FW/
DW) ratio was significantly lower in plants exposed to EEE, indicating possible cell wall stiffening.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022
T1  - The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1848
ER  - 

@conference{
author = "Milić, Dejana and Pantelić, Ana and Banović Đeri, Bojana and Samardžić, Jelena and Vidović, Marija",
year = "2022",
abstract = "In the global warming era, we are facing extreme changes in environmental conditions such
as high and low temperatures, high light and ultraviolet radiation intensity, as well as drought
and increased CO2 levels. The effects of these factors on plants are often interrelated and usually
result in a disturbed balance between the amount of energy received and the ability to process
it. In order to balance energy input and prevent photooxidative damage, plants have evolved
multiple mechanisms for energy dissipation and photoprotection. To efficiently dissipate the excess
excitation energy (EEE), the additional electron sinks, such as photorespiration, biosynthesis
of phenolics and nitrate reduction are stimulated. Variegated leaves of Pelargonium zonale have
been proven to be a suitable model system for examining ‘source–sink’ interactions within the
same leaf, providing the same microenvironment conditions, unlike common root-shoot studies.
The aim of our study was to investigate carbon and nitrogen reallocation between photosynthetically
active (green leaf sectors) and photosynthetically inactive (white leaf sectors) under EEE
pressure induced by low temperature in combination with high light intensity. Besides the amino
acids and phenolics analyses, we monitored morphological and photosynthetic parameters of
P. zonale leaves. Our results revealed higher content of free proteogenic amino acids in the white
leaf sectors than in the green ones. Moreover, EEE triggered increased polyphenol synthesis, namely
anthocyanins, already on the fourth day of the experiment. Also, the leaf fresh/dry weight (FW/
DW) ratio was significantly lower in plants exposed to EEE, indicating possible cell wall stiffening.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022",
title = "The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study",
pages = "66",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1848"
}

Milić, D., Pantelić, A., Banović Đeri, B., Samardžić, J.,& Vidović, M.. (2022). The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 66.
https://hdl.handle.net/21.15107/rcub_imagine_1848

Milić D, Pantelić A, Banović Đeri B, Samardžić J, Vidović M. The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022. 2022;:66.
https://hdl.handle.net/21.15107/rcub_imagine_1848 .

Milić, Dejana, Pantelić, Ana, Banović Đeri, Bojana, Samardžić, Jelena, Vidović, Marija, "The role of the sink tissue in variegated Pelargonium zonale under excess excitation energy pressure morphologic, photosynthetic and metabolic study" in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022 (2022):66,
https://hdl.handle.net/21.15107/rcub_imagine_1848 .

TY  - JOUR
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1604
AB  - The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO center dot activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study
IS  - 9
VL  - 11
DO  - 10.3390/plants11091199
ER  - 

@article{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO center dot activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study",
number = "9",
volume = "11",
doi = "10.3390/plants11091199"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović-Jovanović, S.. (2022). Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. in Plants-Basel
MDPI, Basel., 11(9).
https://doi.org/10.3390/plants11091199

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović-Jovanović S. Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. in Plants-Basel. 2022;11(9).
doi:10.3390/plants11091199 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović-Jovanović, Sonja, "Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study" in Plants-Basel, 11, no. 9 (2022),
https://doi.org/10.3390/plants11091199 . .