TY  - JOUR
AU  - Milovanovic, V.
AU  - Topic, A.
AU  - Milinkovic, N.
AU  - Lazic, Z.
AU  - Ivosevic, A.
AU  - Radojkovic, D.
AU  - Divac Rankov, Aleksandra
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1647
AB  - ObjectiveChronic obstructive pulmonary disease (COPD) is multi–factorial disorder which results from environmental influences and genetic factors. We aimed to investigate whether methionine sulfoxide reductase A (MSRA) rs10903323 gene polymorphism is associated with COPD development and severity in Serbian adult population.MethodsThe study included 155 patients with COPD and 134 healthy volunteers. Genotyping was determined performing home-made polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The difference between the inhibitory activities of normal and oxidized Alpha-1-Antitrypsin (A1AT) against elastase and trypsin was used for determination of Oxidized Alpha-1-Antitrypsin (OxyA1AT) (expressed as % and g/L). Functional activity of A1AT was presented as a specific inhibitor activity to elastase (SIA-Elastase, kU/g).ResultsFrequencies of the genotypes AA, AG and GG were 80.0%, 20.0%, 0% in COPD patients and 80.5%, 18.5% and 1.5% in the control group, and there was no significant difference in genotype or allele distributions between groups. Serum level of A1AT (g/L) and OxyA1AT was significantly higher in COPD patients than in the control group, but functional activity of A1AT (SIA-Elastase) was significantly lower in COPD patients than in the control group. In COPD group, increased level of OxyA1AT was present in G allele carriers who were smokers relative to G allele carriers who were not smokers. In the smoker group of patients with severe and very severe COPD (GOLD3+4), significant increase in OxyA1AT level was present in G allele carriers compared to AA homozygotes.ConclusionThese findings suggest that MSRA rs10903323 gene polymorphism is probably not a risk for COPD by itself but could represent a COPD modifier, since minor, G allele, is associated with an increased level of oxidized A1AT, indicating impaired ability of MSRA to repair oxidized A1AT in COPD-smokers, and in severe form of COPD.
PB  - Elsevier
T2  - Pulmonology
T2  - Pulmonology
T1  - Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients
EP  - 129
IS  - 2
SP  - 122
VL  - 30
DO  - 10.1016/j.pulmoe.2021.09.003
ER  - 

@article{
author = "Milovanovic, V. and Topic, A. and Milinkovic, N. and Lazic, Z. and Ivosevic, A. and Radojkovic, D. and Divac Rankov, Aleksandra",
year = "2024",
abstract = "ObjectiveChronic obstructive pulmonary disease (COPD) is multi–factorial disorder which results from environmental influences and genetic factors. We aimed to investigate whether methionine sulfoxide reductase A (MSRA) rs10903323 gene polymorphism is associated with COPD development and severity in Serbian adult population.MethodsThe study included 155 patients with COPD and 134 healthy volunteers. Genotyping was determined performing home-made polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The difference between the inhibitory activities of normal and oxidized Alpha-1-Antitrypsin (A1AT) against elastase and trypsin was used for determination of Oxidized Alpha-1-Antitrypsin (OxyA1AT) (expressed as % and g/L). Functional activity of A1AT was presented as a specific inhibitor activity to elastase (SIA-Elastase, kU/g).ResultsFrequencies of the genotypes AA, AG and GG were 80.0%, 20.0%, 0% in COPD patients and 80.5%, 18.5% and 1.5% in the control group, and there was no significant difference in genotype or allele distributions between groups. Serum level of A1AT (g/L) and OxyA1AT was significantly higher in COPD patients than in the control group, but functional activity of A1AT (SIA-Elastase) was significantly lower in COPD patients than in the control group. In COPD group, increased level of OxyA1AT was present in G allele carriers who were smokers relative to G allele carriers who were not smokers. In the smoker group of patients with severe and very severe COPD (GOLD3+4), significant increase in OxyA1AT level was present in G allele carriers compared to AA homozygotes.ConclusionThese findings suggest that MSRA rs10903323 gene polymorphism is probably not a risk for COPD by itself but could represent a COPD modifier, since minor, G allele, is associated with an increased level of oxidized A1AT, indicating impaired ability of MSRA to repair oxidized A1AT in COPD-smokers, and in severe form of COPD.",
publisher = "Elsevier",
journal = "Pulmonology, Pulmonology",
title = "Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients",
pages = "129-122",
number = "2",
volume = "30",
doi = "10.1016/j.pulmoe.2021.09.003"
}

Milovanovic, V., Topic, A., Milinkovic, N., Lazic, Z., Ivosevic, A., Radojkovic, D.,& Divac Rankov, A.. (2024). Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients. in Pulmonology
Elsevier., 30(2), 122-129.
https://doi.org/10.1016/j.pulmoe.2021.09.003

Milovanovic V, Topic A, Milinkovic N, Lazic Z, Ivosevic A, Radojkovic D, Divac Rankov A. Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients. in Pulmonology. 2024;30(2):122-129.
doi:10.1016/j.pulmoe.2021.09.003 .

Milovanovic, V., Topic, A., Milinkovic, N., Lazic, Z., Ivosevic, A., Radojkovic, D., Divac Rankov, Aleksandra, "Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients" in Pulmonology, 30, no. 2 (2024):122-129,
https://doi.org/10.1016/j.pulmoe.2021.09.003 . .

TY  - JOUR
AU  - Divac Rankov, Aleksandra
AU  - Jovanović Stojanov, Sofija
AU  - Dragoj, Miodrag
AU  - Ljujić, Mila
PY  - 2023
UR  - https://doi.org/10.1007/s00418-022-02172-3
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2243
AB  - Identification of the signature molecular profiles involved in therapy resistance is of vital importance in developing new strategies for treatments and disease monitoring. Protein alpha-1 antitrypsin (AAT, encoded by SERPINA1 gene) is an acute-phase protein, and its high expression has been linked with unfavorable clinical outcome in different types of cancer; however, data on its involvement in therapy resistance are still insufficient. We analyzed SERPINA1 mRNA expression in three different multidrug-resistant (MDR) cell lines—U87-TxR, NCI-H460/R, and DLD1-TxR—and in U87 cells grown in alginate microfibers as a 3D cellular model of glioblastoma. Expression of IL-6 as a major modulator of SERPINA1 was also analyzed. Additionally, AAT protein expression in MDR cells was analyzed by immunofluorescence. SERPINA1 gene expression and AAT protein expression were significantly upregulated in all the tested MDR cell lines compared with their sensitive counterparts. Moreover, SERPINA1 was significantly upregulated in 3D models of glioblastoma, previously found to have upregulated drug-resistance-related gene expression compared with 2D cells. With the exception of NCI-H460/R, in all cell lines as well as in a 3D model of U87 cells, increase in SERPINA1 expression correlated with the increase in IL-6 expression. Our results indicate that AAT could be utilized as a biomarker of therapy resistance in cancer; however, further studies are needed to elucidate the mechanisms driving AAT upregulation in therapy resistance and its biological significance in this process.
T2  - Histochemistry and Cell Biology
T1  - Alpha-1 antitrypsin expression is upregulated in multidrug-resistant cancer cells
EP  - 437
IS  - 5
SP  - 431
VL  - 159
DO  - 10.1007/s00418-022-02172-3
ER  - 

@article{
author = "Divac Rankov, Aleksandra and Jovanović Stojanov, Sofija and Dragoj, Miodrag and Ljujić, Mila",
year = "2023",
abstract = "Identification of the signature molecular profiles involved in therapy resistance is of vital importance in developing new strategies for treatments and disease monitoring. Protein alpha-1 antitrypsin (AAT, encoded by SERPINA1 gene) is an acute-phase protein, and its high expression has been linked with unfavorable clinical outcome in different types of cancer; however, data on its involvement in therapy resistance are still insufficient. We analyzed SERPINA1 mRNA expression in three different multidrug-resistant (MDR) cell lines—U87-TxR, NCI-H460/R, and DLD1-TxR—and in U87 cells grown in alginate microfibers as a 3D cellular model of glioblastoma. Expression of IL-6 as a major modulator of SERPINA1 was also analyzed. Additionally, AAT protein expression in MDR cells was analyzed by immunofluorescence. SERPINA1 gene expression and AAT protein expression were significantly upregulated in all the tested MDR cell lines compared with their sensitive counterparts. Moreover, SERPINA1 was significantly upregulated in 3D models of glioblastoma, previously found to have upregulated drug-resistance-related gene expression compared with 2D cells. With the exception of NCI-H460/R, in all cell lines as well as in a 3D model of U87 cells, increase in SERPINA1 expression correlated with the increase in IL-6 expression. Our results indicate that AAT could be utilized as a biomarker of therapy resistance in cancer; however, further studies are needed to elucidate the mechanisms driving AAT upregulation in therapy resistance and its biological significance in this process.",
journal = "Histochemistry and Cell Biology",
title = "Alpha-1 antitrypsin expression is upregulated in multidrug-resistant cancer cells",
pages = "437-431",
number = "5",
volume = "159",
doi = "10.1007/s00418-022-02172-3"
}

Divac Rankov, A., Jovanović Stojanov, S., Dragoj, M.,& Ljujić, M.. (2023). Alpha-1 antitrypsin expression is upregulated in multidrug-resistant cancer cells. in Histochemistry and Cell Biology, 159(5), 431-437.
https://doi.org/10.1007/s00418-022-02172-3

Divac Rankov A, Jovanović Stojanov S, Dragoj M, Ljujić M. Alpha-1 antitrypsin expression is upregulated in multidrug-resistant cancer cells. in Histochemistry and Cell Biology. 2023;159(5):431-437.
doi:10.1007/s00418-022-02172-3 .

Divac Rankov, Aleksandra, Jovanović Stojanov, Sofija, Dragoj, Miodrag, Ljujić, Mila, "Alpha-1 antitrypsin expression is upregulated in multidrug-resistant cancer cells" in Histochemistry and Cell Biology, 159, no. 5 (2023):431-437,
https://doi.org/10.1007/s00418-022-02172-3 . .

TY  - CONF
AU  - Nikolić, Dragana
AU  - Divac Rankov, Aleksandra
AU  - Samardžić, Jelena
AU  - Pantelić, Ana
AU  - Spasovski, Vesna
AU  - Banović Đeri, Bojana
AU  - Kosanović, Maja
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2266
AB  - Outer membrane vesicles (OMVs), extracellular vesicles (EVs) produced by Gram-negative bacteria, are
increasingly recognised as promising tools in biomedicine due to their innate ability to interact with human
cells and trigger immune responses. The interaction of OMVs of plant growth-promoting bacteria (PGPB) with
plants, as well as with plant-pathogenic microorganisms, is far less explored. Considering the great
importance of PGPBs for the development of sustainable, environmentally friendly solutions in agriculture, the
study of the role of OMVs in PGPB-plant and PGPB-phytopathogen interactions holds valuable application
potential.
To investigate PGPB OMVs, we isolated and characterised OMVs produced by Paraburkholderia phytofirmans
PsJN, a PGPB strain known to enhance plant resistance to various abiotic and biotic stresses. After testing
different methods for isolating and purifying OMVs, a commercially available affinity-based column system
was selected as the most efficient. Outer membrane origin of isolated OMVs was confirmed using an essay for
detection of lipopolysaccharide (LPS).
To examine the interaction of OMVs with plant cells, Arabidopsis thaliana roots were incubated with isolated
P. phytofirmans PsJN vesicles, previously labelled with lipid binding fluorescent dye Vybrant™ DiD. Red signals
were observed, under confocal laser scanning microscope, in root hairs and root surface in DiD-OMV treated
plants, while in control-treated roots the same signals were missing. The results suggest direct contact of
OMVs with root hairs, which are necessary for nutrient acquisition and plant-microbe interactions in
rhizosphere. Our further research is focused on the characterization of OMV-associated RNA and its potential
delivery into host plant cells.
PB  - Serbian Society for Extracellular Vesicles (SrbEVs)
PB  - Austrian Society for Extracellular Vesicles (ASEV)
PB  - Hungarian Society for Extracellular Vesicles (HSEV)
PB  - Slovenian Network for Extracellular Vesicles (SiN-EV)
C3  - Small New World 2.0
T1  - Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots
EP  - 109
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2266
ER  - 

@conference{
author = "Nikolić, Dragana and Divac Rankov, Aleksandra and Samardžić, Jelena and Pantelić, Ana and Spasovski, Vesna and Banović Đeri, Bojana and Kosanović, Maja",
year = "2023",
abstract = "Outer membrane vesicles (OMVs), extracellular vesicles (EVs) produced by Gram-negative bacteria, are
increasingly recognised as promising tools in biomedicine due to their innate ability to interact with human
cells and trigger immune responses. The interaction of OMVs of plant growth-promoting bacteria (PGPB) with
plants, as well as with plant-pathogenic microorganisms, is far less explored. Considering the great
importance of PGPBs for the development of sustainable, environmentally friendly solutions in agriculture, the
study of the role of OMVs in PGPB-plant and PGPB-phytopathogen interactions holds valuable application
potential.
To investigate PGPB OMVs, we isolated and characterised OMVs produced by Paraburkholderia phytofirmans
PsJN, a PGPB strain known to enhance plant resistance to various abiotic and biotic stresses. After testing
different methods for isolating and purifying OMVs, a commercially available affinity-based column system
was selected as the most efficient. Outer membrane origin of isolated OMVs was confirmed using an essay for
detection of lipopolysaccharide (LPS).
To examine the interaction of OMVs with plant cells, Arabidopsis thaliana roots were incubated with isolated
P. phytofirmans PsJN vesicles, previously labelled with lipid binding fluorescent dye Vybrant™ DiD. Red signals
were observed, under confocal laser scanning microscope, in root hairs and root surface in DiD-OMV treated
plants, while in control-treated roots the same signals were missing. The results suggest direct contact of
OMVs with root hairs, which are necessary for nutrient acquisition and plant-microbe interactions in
rhizosphere. Our further research is focused on the characterization of OMV-associated RNA and its potential
delivery into host plant cells.",
publisher = "Serbian Society for Extracellular Vesicles (SrbEVs), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), Slovenian Network for Extracellular Vesicles (SiN-EV)",
journal = "Small New World 2.0",
title = "Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots",
pages = "109-109",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2266"
}

Nikolić, D., Divac Rankov, A., Samardžić, J., Pantelić, A., Spasovski, V., Banović Đeri, B.,& Kosanović, M.. (2023). Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots. in Small New World 2.0
Serbian Society for Extracellular Vesicles (SrbEVs)., 109-109.
https://hdl.handle.net/21.15107/rcub_imagine_2266

Nikolić D, Divac Rankov A, Samardžić J, Pantelić A, Spasovski V, Banović Đeri B, Kosanović M. Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots. in Small New World 2.0. 2023;:109-109.
https://hdl.handle.net/21.15107/rcub_imagine_2266 .

Nikolić, Dragana, Divac Rankov, Aleksandra, Samardžić, Jelena, Pantelić, Ana, Spasovski, Vesna, Banović Đeri, Bojana, Kosanović, Maja, "Exploring the interaction of Outer membrane vesicles (OMVs) produced by Paraburkholderia phytofirmans PsJN with Arabidopsis thaliana roots" in Small New World 2.0 (2023):109-109,
https://hdl.handle.net/21.15107/rcub_imagine_2266 .

TY  - CONF
AU  - Ljujić, Mila
AU  - Trifunović, Sara
AU  - Ilić, Bojan
AU  - Milovanović, Jelena
AU  - Dinić, Jelena
AU  - Divac Rankov, Aleksandra
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2130
AB  - Introduction: Cigarette smoke exposure is a known risk factor for development of lung diseases and
electronic cigarettes(e-cigarettes) were introduced as a popular and safer alternative to combustible tobacco products. Increasing number of studies are reporting their adverse biological effects both in vivo
and in vitro. Aim of this study was to evaluate the effect of e-cigarettes on mitochondrial function in
lung bronchial epithelial cells.
Methods: Electronic cigarette vapor condensate (ECC) was generated using an e-cigarette device on a
suction trap cooled in a dry ice/ethanol bath.We used unflavoured and flavoured e-cigarette liquids with
and without nicotine. Human bronchial epithelial BEAS2B cells were seeded in 96well plates and treated
with 2% e-cigarette vapour condensate for 24h. Mitochondrial membrane potential was measured using
50nM TMRE (Tetramethyl rhodamine ethyl ester) and cells were visualized on ImageXpress® Pico Automated Cell Imaging System (Molecular Devices, San Jose, CA, USA) with a 10x objective.
Results: We found a significant reduction of TMRE fluorescence in treated cells compared to the control. Imaging of treated cells also revealed changes in cell morphology and the presence of mitochondria in TNT-like structures.
Conclusion: Mitochondrial dysfunction has been associated with various pathological conditions including lung diseases such as asthma, COPD and lung cancer. Due to their relative novelty, the role of
electronic cigarette use in development of chronic lung diseasesisstill relatively unknown. Our findings
contribute to the growing list of studies pointing to their adverse biological effects and imply their involvement in processes contributing to chronic lung diseases.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cell
EP  - 139
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2130
ER  - 

@conference{
author = "Ljujić, Mila and Trifunović, Sara and Ilić, Bojan and Milovanović, Jelena and Dinić, Jelena and Divac Rankov, Aleksandra",
year = "2023",
abstract = "Introduction: Cigarette smoke exposure is a known risk factor for development of lung diseases and
electronic cigarettes(e-cigarettes) were introduced as a popular and safer alternative to combustible tobacco products. Increasing number of studies are reporting their adverse biological effects both in vivo
and in vitro. Aim of this study was to evaluate the effect of e-cigarettes on mitochondrial function in
lung bronchial epithelial cells.
Methods: Electronic cigarette vapor condensate (ECC) was generated using an e-cigarette device on a
suction trap cooled in a dry ice/ethanol bath.We used unflavoured and flavoured e-cigarette liquids with
and without nicotine. Human bronchial epithelial BEAS2B cells were seeded in 96well plates and treated
with 2% e-cigarette vapour condensate for 24h. Mitochondrial membrane potential was measured using
50nM TMRE (Tetramethyl rhodamine ethyl ester) and cells were visualized on ImageXpress® Pico Automated Cell Imaging System (Molecular Devices, San Jose, CA, USA) with a 10x objective.
Results: We found a significant reduction of TMRE fluorescence in treated cells compared to the control. Imaging of treated cells also revealed changes in cell morphology and the presence of mitochondria in TNT-like structures.
Conclusion: Mitochondrial dysfunction has been associated with various pathological conditions including lung diseases such as asthma, COPD and lung cancer. Due to their relative novelty, the role of
electronic cigarette use in development of chronic lung diseasesisstill relatively unknown. Our findings
contribute to the growing list of studies pointing to their adverse biological effects and imply their involvement in processes contributing to chronic lung diseases.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cell",
pages = "139-139",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2130"
}

Ljujić, M., Trifunović, S., Ilić, B., Milovanović, J., Dinić, J.,& Divac Rankov, A.. (2023). Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cell. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 139-139.
https://hdl.handle.net/21.15107/rcub_imagine_2130

Ljujić M, Trifunović S, Ilić B, Milovanović J, Dinić J, Divac Rankov A. Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cell. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:139-139.
https://hdl.handle.net/21.15107/rcub_imagine_2130 .

Ljujić, Mila, Trifunović, Sara, Ilić, Bojan, Milovanović, Jelena, Dinić, Jelena, Divac Rankov, Aleksandra, "Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cell" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):139-139,
https://hdl.handle.net/21.15107/rcub_imagine_2130 .

TY  - CONF
AU  - Ljujić, Mila
AU  - Milovanović, Jelena
AU  - Ilić, Bojan
AU  - Trifunović, Sara
AU  - Divac Rankov, Aleksandra
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2131
AB  - Introduction: E-cigarettes are becoming increasingly popular, but their potentially harmful effects are
not well studied. Although they are used to aid smoking cessation, there is evidence that the chemicals
they contain can affect lung cells, and the effects may also be important for lung cancer cells. Our objective wasto investigate the effects of e-cigarette vapor condensate on mitochondrial function of A549
cells.
Methods: We used the Agilent Seahorse Mito Stress Test, according to the manufacturer’s protocol to
evaluate the mitochondrial function of A549 cells under the treatment with different concentrations of
e-cigarette vapor condensates. Vapor condensates were prepared from e-cigarette liquid base (PG/VG),
base with nicotine (PG/VG+N), with flavoring (PG/VG+F), or with nicotine and flavoring (PG /VG+F+N).
The cells were exposed to 2% or 3% PG /VG, PG /VG+F, PG/VG+N, and PG/VG+F+N for 24 hours before
testing.
Results: All treatments with 2% vapor condensates affected mitochondrial function of A549 cells. Basal
and maximal respiration were decreased, indicating mitochondrial dysfunction. Higher concentrations
of vapor condensate (3%) significantly increased proton leak and decreased mitochondrial coupling efficiency, indicating mitochondrial damage. However, the increased spare respiratory capacity, in 3%
vapor condensate treated cells, may indicate activation of a compensatory response in mitochondria.
Conclusion: Our resultssuggest that e-cigarette vapor condensate may have deleterious effects on mitochondria. Further analysis of mitochondrial function and morphology would further elucidate the effects of e-cigarette vapor condensate.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - E-cigarette vapor condensate affects mitochondrial function in A549 cells
EP  - 140
SP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2131
ER  - 

@conference{
author = "Ljujić, Mila and Milovanović, Jelena and Ilić, Bojan and Trifunović, Sara and Divac Rankov, Aleksandra",
year = "2023",
abstract = "Introduction: E-cigarettes are becoming increasingly popular, but their potentially harmful effects are
not well studied. Although they are used to aid smoking cessation, there is evidence that the chemicals
they contain can affect lung cells, and the effects may also be important for lung cancer cells. Our objective wasto investigate the effects of e-cigarette vapor condensate on mitochondrial function of A549
cells.
Methods: We used the Agilent Seahorse Mito Stress Test, according to the manufacturer’s protocol to
evaluate the mitochondrial function of A549 cells under the treatment with different concentrations of
e-cigarette vapor condensates. Vapor condensates were prepared from e-cigarette liquid base (PG/VG),
base with nicotine (PG/VG+N), with flavoring (PG/VG+F), or with nicotine and flavoring (PG /VG+F+N).
The cells were exposed to 2% or 3% PG /VG, PG /VG+F, PG/VG+N, and PG/VG+F+N for 24 hours before
testing.
Results: All treatments with 2% vapor condensates affected mitochondrial function of A549 cells. Basal
and maximal respiration were decreased, indicating mitochondrial dysfunction. Higher concentrations
of vapor condensate (3%) significantly increased proton leak and decreased mitochondrial coupling efficiency, indicating mitochondrial damage. However, the increased spare respiratory capacity, in 3%
vapor condensate treated cells, may indicate activation of a compensatory response in mitochondria.
Conclusion: Our resultssuggest that e-cigarette vapor condensate may have deleterious effects on mitochondria. Further analysis of mitochondrial function and morphology would further elucidate the effects of e-cigarette vapor condensate.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "E-cigarette vapor condensate affects mitochondrial function in A549 cells",
pages = "140-140",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2131"
}

Ljujić, M., Milovanović, J., Ilić, B., Trifunović, S.,& Divac Rankov, A.. (2023). E-cigarette vapor condensate affects mitochondrial function in A549 cells. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 140-140.
https://hdl.handle.net/21.15107/rcub_imagine_2131

Ljujić M, Milovanović J, Ilić B, Trifunović S, Divac Rankov A. E-cigarette vapor condensate affects mitochondrial function in A549 cells. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:140-140.
https://hdl.handle.net/21.15107/rcub_imagine_2131 .

Ljujić, Mila, Milovanović, Jelena, Ilić, Bojan, Trifunović, Sara, Divac Rankov, Aleksandra, "E-cigarette vapor condensate affects mitochondrial function in A549 cells" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):140-140,
https://hdl.handle.net/21.15107/rcub_imagine_2131 .

TY  - CONF
AU  - Divac Rankov, Aleksandra
AU  - Ušjak, Dušan
AU  - Mitić, Martina Mia
AU  - Kusić Tisma, Jelena
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1985
AB  - Cystic fibrosis (CF) is a monogenic autosomal recessive disease caused by mutations in
transmembrane conductance regulator (CFTR) gene. The golden standard for the diagnosis
of CF is sweat chloride testing (>60 mmol/L) together with the identification of two CFcausing
variants of CFTR gene. Nevertheless, about 0.01% of patients with elevated sweat
chloride and high clinical suspicion of CF do not carry any CF-causing variants.
Here we present analysis of whole exome sequencing (WES) results for two patients with
elevated sweat chloride levels and clinical presentation of CF in whom no CF-causing
mutations were detected after CFTR gene whole coding region sequencing, and large
insertion/deletion testing.
Genomic DNA was extracted from whole blood, subjected to library preparation using
DNA nanoball technology from BGI and sequenced on DNBSEQ-G400 (MGI). Produced
fastq files were mapped to hg38 reference genome using BWA/SAM tools. VCF files were
generated using GATK (BaseRecalibrator, HaplotypeCaller) and annotated with InterVar
and AnnoVar tools. Filtering of detected variants for disease relevance was done using
the following criteria: QC Filter, GnomAD Allele Frequency, Functional consequences and
phenotype-genotype relationship.
In both patients, similar number of variants predicted to impair protein function were
detected (27 and 25). In two genes (CACNA1H and MUC5B) missense type variants
were found in both patients and loss of function variants were found in 7 and 11 genes,
respectively. Functional assessment of selected variants is underway.
Bioinformatics analyses are a valuable tool enabling identification of underlining genetic
bases of disease phenotype, important in the context of optimal patient management and
targeted therapies.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Decoding Cystic Fibrosis Phenotype
EP  - 44
SP  - 44
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1985
ER  - 

@conference{
author = "Divac Rankov, Aleksandra and Ušjak, Dušan and Mitić, Martina Mia and Kusić Tisma, Jelena",
year = "2023",
abstract = "Cystic fibrosis (CF) is a monogenic autosomal recessive disease caused by mutations in
transmembrane conductance regulator (CFTR) gene. The golden standard for the diagnosis
of CF is sweat chloride testing (>60 mmol/L) together with the identification of two CFcausing
variants of CFTR gene. Nevertheless, about 0.01% of patients with elevated sweat
chloride and high clinical suspicion of CF do not carry any CF-causing variants.
Here we present analysis of whole exome sequencing (WES) results for two patients with
elevated sweat chloride levels and clinical presentation of CF in whom no CF-causing
mutations were detected after CFTR gene whole coding region sequencing, and large
insertion/deletion testing.
Genomic DNA was extracted from whole blood, subjected to library preparation using
DNA nanoball technology from BGI and sequenced on DNBSEQ-G400 (MGI). Produced
fastq files were mapped to hg38 reference genome using BWA/SAM tools. VCF files were
generated using GATK (BaseRecalibrator, HaplotypeCaller) and annotated with InterVar
and AnnoVar tools. Filtering of detected variants for disease relevance was done using
the following criteria: QC Filter, GnomAD Allele Frequency, Functional consequences and
phenotype-genotype relationship.
In both patients, similar number of variants predicted to impair protein function were
detected (27 and 25). In two genes (CACNA1H and MUC5B) missense type variants
were found in both patients and loss of function variants were found in 7 and 11 genes,
respectively. Functional assessment of selected variants is underway.
Bioinformatics analyses are a valuable tool enabling identification of underlining genetic
bases of disease phenotype, important in the context of optimal patient management and
targeted therapies.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Decoding Cystic Fibrosis Phenotype",
pages = "44-44",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1985"
}

Divac Rankov, A., Ušjak, D., Mitić, M. M.,& Kusić Tisma, J.. (2023). Decoding Cystic Fibrosis Phenotype. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 44-44.
https://hdl.handle.net/21.15107/rcub_imagine_1985

Divac Rankov A, Ušjak D, Mitić MM, Kusić Tisma J. Decoding Cystic Fibrosis Phenotype. in 4th Belgrade Bioinformatics Conference. 2023;4:44-44.
https://hdl.handle.net/21.15107/rcub_imagine_1985 .

Divac Rankov, Aleksandra, Ušjak, Dušan, Mitić, Martina Mia, Kusić Tisma, Jelena, "Decoding Cystic Fibrosis Phenotype" in 4th Belgrade Bioinformatics Conference, 4 (2023):44-44,
https://hdl.handle.net/21.15107/rcub_imagine_1985 .

TY  - CONF
AU  - Divac Rankov, Aleksandra
AU  - Kušić-Tišma, Jelena
AU  - Đorđević, Valentina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2177
AB  - Bioinformatics was established at the IMGGE
in collaboration with the Beijing Genomics Institute
(BGI) and with the support of the Government of
the Republic of Serbia. The Center was founded
with the aim of accelerating the implementation
of 4P medicine (Preventive, Predictive, Personalized,
and Participatory), in our country by using the
cutting-edge tools of molecular biology and information
technology. As such, the center is unique in
Serbia and the South East Europe region.
The state-of-the-art equipment existing at the
Center (DNBSEQ-G400 (BGI), NextSeq 550Dx,
2000 and MiSeq (Illumina) Sequencing Systems;
MinION (OxfordNanopore); MGISP-9600
High-throughput Automated Sample Preparation
System) offered a wide range of application: the
whole genome and whole exome sequencing, targeted
sequencing, transcriptome sequencing and
more. The analysis of data was facilitated by the
access to the National Platform for Artificial Intelligence providing space for secure data storage, and
to a supercomputer (Nvidia), critical for processing
and analyzing Big Data.
Since its establishment more than 2000
SARS-CoV2 genomes were sequenced, 300 patient
samples undervent diagnostic work-up. Pilot project
for NIFTYPro screening was finished encompasing
58 pregnant women. First transcriptomes
and metagenomes were analyzed.
Further implementation of NGS methodology
in research and for diagnostics, and intensive
development of bioinformatics, by strengthening
the hardware and software capacities and the education
of the bioinformatics team, will lead us in
becoming driving force of the development of biomedicine
and biotechnology in Serbia, extending
collaboration beyond our borders.
PB  - Macedonian Academy of Sciences and Arts
C3  - International Journal of Medical Genetics
T1  - STRENGTHENING THE NEXT-GENERATION SEQUENCING AND BIOINFORMATICS CAPACITIES IN THE REPUBLIC OF SERBIA
EP  - 75
IS  - Supplement
SP  - 75
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2177
ER  - 

@conference{
author = "Divac Rankov, Aleksandra and Kušić-Tišma, Jelena and Đorđević, Valentina",
year = "2023",
abstract = "Bioinformatics was established at the IMGGE
in collaboration with the Beijing Genomics Institute
(BGI) and with the support of the Government of
the Republic of Serbia. The Center was founded
with the aim of accelerating the implementation
of 4P medicine (Preventive, Predictive, Personalized,
and Participatory), in our country by using the
cutting-edge tools of molecular biology and information
technology. As such, the center is unique in
Serbia and the South East Europe region.
The state-of-the-art equipment existing at the
Center (DNBSEQ-G400 (BGI), NextSeq 550Dx,
2000 and MiSeq (Illumina) Sequencing Systems;
MinION (OxfordNanopore); MGISP-9600
High-throughput Automated Sample Preparation
System) offered a wide range of application: the
whole genome and whole exome sequencing, targeted
sequencing, transcriptome sequencing and
more. The analysis of data was facilitated by the
access to the National Platform for Artificial Intelligence providing space for secure data storage, and
to a supercomputer (Nvidia), critical for processing
and analyzing Big Data.
Since its establishment more than 2000
SARS-CoV2 genomes were sequenced, 300 patient
samples undervent diagnostic work-up. Pilot project
for NIFTYPro screening was finished encompasing
58 pregnant women. First transcriptomes
and metagenomes were analyzed.
Further implementation of NGS methodology
in research and for diagnostics, and intensive
development of bioinformatics, by strengthening
the hardware and software capacities and the education
of the bioinformatics team, will lead us in
becoming driving force of the development of biomedicine
and biotechnology in Serbia, extending
collaboration beyond our borders.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "International Journal of Medical Genetics",
title = "STRENGTHENING THE NEXT-GENERATION SEQUENCING AND BIOINFORMATICS CAPACITIES IN THE REPUBLIC OF SERBIA",
pages = "75-75",
number = "Supplement",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2177"
}

Divac Rankov, A., Kušić-Tišma, J.,& Đorđević, V.. (2023). STRENGTHENING THE NEXT-GENERATION SEQUENCING AND BIOINFORMATICS CAPACITIES IN THE REPUBLIC OF SERBIA. in International Journal of Medical Genetics
Macedonian Academy of Sciences and Arts., 26(Supplement), 75-75.
https://hdl.handle.net/21.15107/rcub_imagine_2177

Divac Rankov A, Kušić-Tišma J, Đorđević V. STRENGTHENING THE NEXT-GENERATION SEQUENCING AND BIOINFORMATICS CAPACITIES IN THE REPUBLIC OF SERBIA. in International Journal of Medical Genetics. 2023;26(Supplement):75-75.
https://hdl.handle.net/21.15107/rcub_imagine_2177 .

Divac Rankov, Aleksandra, Kušić-Tišma, Jelena, Đorđević, Valentina, "STRENGTHENING THE NEXT-GENERATION SEQUENCING AND BIOINFORMATICS CAPACITIES IN THE REPUBLIC OF SERBIA" in International Journal of Medical Genetics, 26, no. Supplement (2023):75-75,
https://hdl.handle.net/21.15107/rcub_imagine_2177 .

TY  - CONF
AU  - Nešić, Sofija
AU  - Divac Rankov, Aleksandra
AU  - Spasovski, Vesna
AU  - Kosanović, Maja
AU  - Nikolić, Dragana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2157
AB  - Extracellular vesicles (EVs) are recognized as important mediators of intercellular
communication in both eukaryotes and prokaryotes. These lipid membrane –
coated spherical nanoparticles carry proteins, nucleic acids and other cellular
products, and facilitate exchange of these biomolecules among cells within an
organism, but also between cells of different organisms, belonging to different
species and even kingdoms. Outer membrane vesicles (OMVs), EVs produced
by Gram-negative bacteria, are a significant mediator of microbial communication,
involved in biofilm formation, virulence, and modulation of host immunity.
OMVs of both pathogenic and plant beneficial bacteria have been shown
to elicit plant immune responses. Investigations on the modes of OMV-plant
cells interactions are still in their infancy, but gain rising attention. Aiming to
monitor the interaction between OMVs of Paraburkholderia phytofirmans PsJN,
a plant growth promoting bacteria, and Arabidopsis thaliana roots, we isolated
OMVs from bacterial culture in mineral medium, using an ion-exchange chromatography
system. Isolated OMVs were labeled with lipid binding fluorescent
dye Vybrant™ DiD and unbound dye was removed by washing vesicles on ultrafiltration
columns. The same dye concentration in phosphate buffer saline,
equivalently washed, was used as a control. A. thaliana roots, grown on Murashige
and Skoog medium, were incubated with DiD-OMVs or control dye/buffer
mixture, washed and observed under confocal laser scanning microscope. Red
signals were observed in root hairs and epidermis in DiD-OMV treated plants,
while in control-treated roots the same signals were missing. The results indicate
direct contact of bacterial vesicles with epidermis and root hairs, which are
indispensable for nutrient acquisition and plant-microbe interactions in rhizosphere.
Further investigation will address the questions of the nature of OMVplant
cell interaction, including potential delivery of OMVs cargo into host plant
cells. Considering that OMVs are increasingly recognized as promising tools in
biomedicine, exploring their potential for agronomical applications would be
highly appreciated.
PB  - Belgrade : Faculty of Biology
C3  - ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
T1  - Outer membrane vesicles of plant beneficial bacterial strain
Paraburkholderia phytofirmans PsJN make a contact with
Arabidopsis thaliana roots
EP  - 89
SP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2157
ER  - 

@conference{
author = "Nešić, Sofija and Divac Rankov, Aleksandra and Spasovski, Vesna and Kosanović, Maja and Nikolić, Dragana",
year = "2023",
abstract = "Extracellular vesicles (EVs) are recognized as important mediators of intercellular
communication in both eukaryotes and prokaryotes. These lipid membrane –
coated spherical nanoparticles carry proteins, nucleic acids and other cellular
products, and facilitate exchange of these biomolecules among cells within an
organism, but also between cells of different organisms, belonging to different
species and even kingdoms. Outer membrane vesicles (OMVs), EVs produced
by Gram-negative bacteria, are a significant mediator of microbial communication,
involved in biofilm formation, virulence, and modulation of host immunity.
OMVs of both pathogenic and plant beneficial bacteria have been shown
to elicit plant immune responses. Investigations on the modes of OMV-plant
cells interactions are still in their infancy, but gain rising attention. Aiming to
monitor the interaction between OMVs of Paraburkholderia phytofirmans PsJN,
a plant growth promoting bacteria, and Arabidopsis thaliana roots, we isolated
OMVs from bacterial culture in mineral medium, using an ion-exchange chromatography
system. Isolated OMVs were labeled with lipid binding fluorescent
dye Vybrant™ DiD and unbound dye was removed by washing vesicles on ultrafiltration
columns. The same dye concentration in phosphate buffer saline,
equivalently washed, was used as a control. A. thaliana roots, grown on Murashige
and Skoog medium, were incubated with DiD-OMVs or control dye/buffer
mixture, washed and observed under confocal laser scanning microscope. Red
signals were observed in root hairs and epidermis in DiD-OMV treated plants,
while in control-treated roots the same signals were missing. The results indicate
direct contact of bacterial vesicles with epidermis and root hairs, which are
indispensable for nutrient acquisition and plant-microbe interactions in rhizosphere.
Further investigation will address the questions of the nature of OMVplant
cell interaction, including potential delivery of OMVs cargo into host plant
cells. Considering that OMVs are increasingly recognized as promising tools in
biomedicine, exploring their potential for agronomical applications would be
highly appreciated.",
publisher = "Belgrade : Faculty of Biology",
journal = "ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture",
title = "Outer membrane vesicles of plant beneficial bacterial strain
Paraburkholderia phytofirmans PsJN make a contact with
Arabidopsis thaliana roots",
pages = "89-89",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2157"
}

Nešić, S., Divac Rankov, A., Spasovski, V., Kosanović, M.,& Nikolić, D.. (2023). Outer membrane vesicles of plant beneficial bacterial strain
Paraburkholderia phytofirmans PsJN make a contact with
Arabidopsis thaliana roots. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
Belgrade : Faculty of Biology., 89-89.
https://hdl.handle.net/21.15107/rcub_imagine_2157

Nešić S, Divac Rankov A, Spasovski V, Kosanović M, Nikolić D. Outer membrane vesicles of plant beneficial bacterial strain
Paraburkholderia phytofirmans PsJN make a contact with
Arabidopsis thaliana roots. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture. 2023;:89-89.
https://hdl.handle.net/21.15107/rcub_imagine_2157 .

Nešić, Sofija, Divac Rankov, Aleksandra, Spasovski, Vesna, Kosanović, Maja, Nikolić, Dragana, "Outer membrane vesicles of plant beneficial bacterial strain
Paraburkholderia phytofirmans PsJN make a contact with
Arabidopsis thaliana roots" in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture (2023):89-89,
https://hdl.handle.net/21.15107/rcub_imagine_2157 .

TY  - CONF
AU  - Kusić Tisma, Jelena
AU  - Ušjak, Dušan
AU  - Mitić, Martina Mia
AU  - Divac Rankov, Aleksandra
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2178
AB  - Background: Cystic fibrosis (CF) is autosomal
recessive disorder characterized by chronic
respiratory problems and poor growth. CF is caused
by defect in transmembrane conductance regulator
(CFTR) protein. CF is diagnosed by sweat chloride
analysis (>60 mmol/L) with the identification of
two CF-causing variants of CFTR gene. With a
longstanding history of CFTR gene analysis, our
laboratory identified several patients with elevated
sweat chloride and clinical manifestations of CF in
whom no CF-causing mutations were detected after
sequencing of whole coding region and testing for
large insertion/deletion of CFTR gene. In order to
elucidate genetic background of conditions that
mimic CF we performed whole exome sequencing
(WES) in two such patients.
Methods: Library preparation was done using
DNA nanoball technology. Produced fastq files
were mapped to hg38. VCF files were generated
using GATK and annotated with InterVar and AnnoVar
tools. Variants filtering for disease relevance was done using the following criteria: QC, GnomAD
Allele Frequency, Functional consequences
and phenotype-genotype relationship.
Results: CACNA1H and MUC5B genes were
found to be impaired in both patients. Similar number
of variants predicted to impair protein function
were detected (27 and 25) in each patient. Loss of
function variants were found in 7 and 11 genes,
respectively.
Conclusion: Further assessment of selected
variants will clarify their functional effect and relevance
for the patient’s clinical phenotype. WES
analysis will help identify genetic aspects of disease
and assist in optimal patient management in about
0.01% of patients with elevated sweat chloride and
high clinical suspicion of CF that do not carry any
CF-causing variants.
PB  - Macedonian Academy of Sciences and Arts
C3  - International Journal of Medical Genetics
T1  - POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE
EP  - 80
IS  - Supplement
SP  - 80
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2178
ER  - 

@conference{
author = "Kusić Tisma, Jelena and Ušjak, Dušan and Mitić, Martina Mia and Divac Rankov, Aleksandra",
year = "2023",
abstract = "Background: Cystic fibrosis (CF) is autosomal
recessive disorder characterized by chronic
respiratory problems and poor growth. CF is caused
by defect in transmembrane conductance regulator
(CFTR) protein. CF is diagnosed by sweat chloride
analysis (>60 mmol/L) with the identification of
two CF-causing variants of CFTR gene. With a
longstanding history of CFTR gene analysis, our
laboratory identified several patients with elevated
sweat chloride and clinical manifestations of CF in
whom no CF-causing mutations were detected after
sequencing of whole coding region and testing for
large insertion/deletion of CFTR gene. In order to
elucidate genetic background of conditions that
mimic CF we performed whole exome sequencing
(WES) in two such patients.
Methods: Library preparation was done using
DNA nanoball technology. Produced fastq files
were mapped to hg38. VCF files were generated
using GATK and annotated with InterVar and AnnoVar
tools. Variants filtering for disease relevance was done using the following criteria: QC, GnomAD
Allele Frequency, Functional consequences
and phenotype-genotype relationship.
Results: CACNA1H and MUC5B genes were
found to be impaired in both patients. Similar number
of variants predicted to impair protein function
were detected (27 and 25) in each patient. Loss of
function variants were found in 7 and 11 genes,
respectively.
Conclusion: Further assessment of selected
variants will clarify their functional effect and relevance
for the patient’s clinical phenotype. WES
analysis will help identify genetic aspects of disease
and assist in optimal patient management in about
0.01% of patients with elevated sweat chloride and
high clinical suspicion of CF that do not carry any
CF-causing variants.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "International Journal of Medical Genetics",
title = "POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE",
pages = "80-80",
number = "Supplement",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2178"
}

Kusić Tisma, J., Ušjak, D., Mitić, M. M.,& Divac Rankov, A.. (2023). POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE. in International Journal of Medical Genetics
Macedonian Academy of Sciences and Arts., 26(Supplement), 80-80.
https://hdl.handle.net/21.15107/rcub_imagine_2178

Kusić Tisma J, Ušjak D, Mitić MM, Divac Rankov A. POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE. in International Journal of Medical Genetics. 2023;26(Supplement):80-80.
https://hdl.handle.net/21.15107/rcub_imagine_2178 .

Kusić Tisma, Jelena, Ušjak, Dušan, Mitić, Martina Mia, Divac Rankov, Aleksandra, "POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE" in International Journal of Medical Genetics, 26, no. Supplement (2023):80-80,
https://hdl.handle.net/21.15107/rcub_imagine_2178 .

TY  - CONF
AU  - Ilić, Bojan
AU  - Ljujić, Mila
AU  - Trifunović, Sara
AU  - Divac Rankov, Aleksandra
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2120
AB  - Introduction: E-cigarettes are advertised as safer alternative to tradicional cigarettes. However, they
contain chemicals that can exhibit toxic effects on the organism. Notably, effects of e-cigarettes on in
utero development are not well studied. We wanted to compare potential toxic effects of e-cigarette liquid and vapor condensate on development of zebrafish embryos.
Methods: Six hour old zebrafish embryos were exposed to different concentrations of e-cigarette liquid
or vapour condensate – 0.1% and 1%. Untreated embryos were used as control. Each treatment and control were set up in triplicate, with at least 20 embryos per treatment. The effects on survival, hatching and
developmental malformations were monitored using light microscopy, at 3 timepoints - 24, 48 and 72
hours post fertilization (hpf).
Results: No noticeable differences between control and treated groups were observed 24 hpf. Hatched
larvae (35%) treated with 0.1% condensate had scoliosis and malformations- yolk sac and pericardial
edema at 48 hpf. In groups treated with 1% of condensate or liquid, hatching was delayed and did not
start 48 hpf. At 72 hpf timepoint, in wells with 1% condensate, less than 30% of larvae hatched in total,
which was comparable to wells with e-cigarette liquid (25%). Malformations that were observed in all
treatements are hemagglutionation, pericardial or yolk sac edema, and scoliosis. In groups with 0.1%
condensate these maformations were observed in lower number of embryos, but the percentage of
hatched larvae was higher (approximately 80%) at 72 hpf.
Conclusions: Chronic exposure to e-cigarette vapor condensate and liquid leads to severe disorders of
zebrafish embryonic development.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish
EP  - 73
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2120
ER  - 

@conference{
author = "Ilić, Bojan and Ljujić, Mila and Trifunović, Sara and Divac Rankov, Aleksandra",
year = "2023",
abstract = "Introduction: E-cigarettes are advertised as safer alternative to tradicional cigarettes. However, they
contain chemicals that can exhibit toxic effects on the organism. Notably, effects of e-cigarettes on in
utero development are not well studied. We wanted to compare potential toxic effects of e-cigarette liquid and vapor condensate on development of zebrafish embryos.
Methods: Six hour old zebrafish embryos were exposed to different concentrations of e-cigarette liquid
or vapour condensate – 0.1% and 1%. Untreated embryos were used as control. Each treatment and control were set up in triplicate, with at least 20 embryos per treatment. The effects on survival, hatching and
developmental malformations were monitored using light microscopy, at 3 timepoints - 24, 48 and 72
hours post fertilization (hpf).
Results: No noticeable differences between control and treated groups were observed 24 hpf. Hatched
larvae (35%) treated with 0.1% condensate had scoliosis and malformations- yolk sac and pericardial
edema at 48 hpf. In groups treated with 1% of condensate or liquid, hatching was delayed and did not
start 48 hpf. At 72 hpf timepoint, in wells with 1% condensate, less than 30% of larvae hatched in total,
which was comparable to wells with e-cigarette liquid (25%). Malformations that were observed in all
treatements are hemagglutionation, pericardial or yolk sac edema, and scoliosis. In groups with 0.1%
condensate these maformations were observed in lower number of embryos, but the percentage of
hatched larvae was higher (approximately 80%) at 72 hpf.
Conclusions: Chronic exposure to e-cigarette vapor condensate and liquid leads to severe disorders of
zebrafish embryonic development.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish",
pages = "73-73",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2120"
}

Ilić, B., Ljujić, M., Trifunović, S.,& Divac Rankov, A.. (2023). E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 73-73.
https://hdl.handle.net/21.15107/rcub_imagine_2120

Ilić B, Ljujić M, Trifunović S, Divac Rankov A. E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:73-73.
https://hdl.handle.net/21.15107/rcub_imagine_2120 .

Ilić, Bojan, Ljujić, Mila, Trifunović, Sara, Divac Rankov, Aleksandra, "E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):73-73,
https://hdl.handle.net/21.15107/rcub_imagine_2120 .

TY  - CONF
AU  - Divac Rankov, Aleksandar
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1748
AB  - Zebrica (Danio rerio) je kao laboratorijski model organizam, isprva korišćena za
izučavanje razvića, a danas je prisutna u gotovo svim naučnim granama, od
toksikologije do istraživanja bolesti čoveka. U poslednje vreme zadobija sve
veći značaj i u istraživanjima bolesti pluća. Riblji mehur, pored toga što ima
isto embrionalno poreklo kao pluća, ima i odgovarajuće strukturne i
molekularne karakteristike što omogućava relevantna ispitivanja nastanka,
progresije i terapije različitih bolesti pluća. Na zebricama je moguće
ispitivati delovanje sredinskih faktora koji utiču na nastanak bolesti pluća,
kao što su duvanski dim i različiti zagađivači. Relativno kratko vreme
generacije i razviće van tela majke omogućavaju inter- i trans-generacijska
ispitivanja delovanja faktora sredine. Transgene reporter linije sa
fluorescentno obeleženim neutrofilima umnogome olakšavaju istraživanja
inflamatornih aspekata bolesti pluća, kao što su astma i hronična
opstruktivna bolest pluća. Postoji nekoliko transgenih modela zebrica za
ispitivanja određenih bolesti pluća (cistična fibroza, deficijencija alfa-1
antitripsina). Ne mali značaj zebrica ima i u ispitivanjima infektivnih
bolesti pluća, od tuberkuloze do kovida. Ksenograft model, koji podrazumeva
ubrizgavanje ćelija različitih tipova humanih karcinoma pluća u embrione
zebrica, omogućava ispitivanja procesa metastaziranja, odgovora na terapiju i
efikasnosti novih lekova. Zbog svega navedenog, zebrica predstavlja značajan
model za istraživanja bolesti pluća.
AB  - Зебрица (Danio rerio) је као лабораторијски модел организам, испрва коришћена за
изучавање развића, а данас је присутна у готово свим научним гранама, од
токсикологије до истраживања болести човека. У последње време задобија све
већи значај и у истраживањима болести плућа. Рибљи мехур, поред тога што има
исто ембрионално порекло као плућа, има и одговарајуће структурне и
молекуларне карактеристике што омогућава релевантна испитивања настанка,
прогресије и терапије различитих болести плућа. На зебрицама је могуће
испитивати деловање срединских фактора који утичу на настанак болести плућа,
као што су дувански дим и различити загађивачи. Релативно кратко време
генерације и развиће ван тела мајке омогућавају интер- и транс-генерацијска
испитивања деловања фактора средине. Трансгене репортер линије са
флуоресцентно обележеним неутрофилима умногоме олакшавају истраживања
инфламаторних аспеката болести плућа, као што су астма и хронична
опструктивна болест плућа. Постоји неколико трансгених модела зебрица за
испитивања одређених болести плућа (цистична фиброза, дефицијенција алфа-1
антитрипсина). Не мали значај зебрица има и у испитивањима инфективних
болести плућа, од туберкулозе до ковида. Ксенографт модел, који подразумева
убризгавање ћелија различитих типова хуманих карцинома плућа у ембрионе
зебрица, омогућава испитивања процеса метастазирања, одговора на терапију и
ефикасности нових лекова. Због свега наведеног, зебрица представља значајан
модел за истраживања болести плућа.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Zebrica (Danio rerio) kao model za izučavanje bolesti pluća
T1  - Зебрица (Danio rerio) као модел за изучавање болести плућа
SP  - 277
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1748
ER  - 

@conference{
author = "Divac Rankov, Aleksandar",
year = "2022",
abstract = "Zebrica (Danio rerio) je kao laboratorijski model organizam, isprva korišćena za
izučavanje razvića, a danas je prisutna u gotovo svim naučnim granama, od
toksikologije do istraživanja bolesti čoveka. U poslednje vreme zadobija sve
veći značaj i u istraživanjima bolesti pluća. Riblji mehur, pored toga što ima
isto embrionalno poreklo kao pluća, ima i odgovarajuće strukturne i
molekularne karakteristike što omogućava relevantna ispitivanja nastanka,
progresije i terapije različitih bolesti pluća. Na zebricama je moguće
ispitivati delovanje sredinskih faktora koji utiču na nastanak bolesti pluća,
kao što su duvanski dim i različiti zagađivači. Relativno kratko vreme
generacije i razviće van tela majke omogućavaju inter- i trans-generacijska
ispitivanja delovanja faktora sredine. Transgene reporter linije sa
fluorescentno obeleženim neutrofilima umnogome olakšavaju istraživanja
inflamatornih aspekata bolesti pluća, kao što su astma i hronična
opstruktivna bolest pluća. Postoji nekoliko transgenih modela zebrica za
ispitivanja određenih bolesti pluća (cistična fibroza, deficijencija alfa-1
antitripsina). Ne mali značaj zebrica ima i u ispitivanjima infektivnih
bolesti pluća, od tuberkuloze do kovida. Ksenograft model, koji podrazumeva
ubrizgavanje ćelija različitih tipova humanih karcinoma pluća u embrione
zebrica, omogućava ispitivanja procesa metastaziranja, odgovora na terapiju i
efikasnosti novih lekova. Zbog svega navedenog, zebrica predstavlja značajan
model za istraživanja bolesti pluća., Зебрица (Danio rerio) је као лабораторијски модел организам, испрва коришћена за
изучавање развића, а данас је присутна у готово свим научним гранама, од
токсикологије до истраживања болести човека. У последње време задобија све
већи значај и у истраживањима болести плућа. Рибљи мехур, поред тога што има
исто ембрионално порекло као плућа, има и одговарајуће структурне и
молекуларне карактеристике што омогућава релевантна испитивања настанка,
прогресије и терапије различитих болести плућа. На зебрицама је могуће
испитивати деловање срединских фактора који утичу на настанак болести плућа,
као што су дувански дим и различити загађивачи. Релативно кратко време
генерације и развиће ван тела мајке омогућавају интер- и транс-генерацијска
испитивања деловања фактора средине. Трансгене репортер линије са
флуоресцентно обележеним неутрофилима умногоме олакшавају истраживања
инфламаторних аспеката болести плућа, као што су астма и хронична
опструктивна болест плућа. Постоји неколико трансгених модела зебрица за
испитивања одређених болести плућа (цистична фиброза, дефицијенција алфа-1
антитрипсина). Не мали значај зебрица има и у испитивањима инфективних
болести плућа, од туберкулозе до ковида. Ксенографт модел, који подразумева
убризгавање ћелија различитих типова хуманих карцинома плућа у ембрионе
зебрица, омогућава испитивања процеса метастазирања, одговора на терапију и
ефикасности нових лекова. Због свега наведеног, зебрица представља значајан
модел за истраживања болести плућа.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Zebrica (Danio rerio) kao model za izučavanje bolesti pluća, Зебрица (Danio rerio) као модел за изучавање болести плућа",
pages = "277",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1748"
}

Divac Rankov, A.. (2022). Zebrica (Danio rerio) kao model za izučavanje bolesti pluća. in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 277.
https://hdl.handle.net/21.15107/rcub_imagine_1748

Divac Rankov A. Zebrica (Danio rerio) kao model za izučavanje bolesti pluća. in Treći kongres biologa Srbije. 2022;:277.
https://hdl.handle.net/21.15107/rcub_imagine_1748 .

Divac Rankov, Aleksandar, "Zebrica (Danio rerio) kao model za izučavanje bolesti pluća" in Treći kongres biologa Srbije (2022):277,
https://hdl.handle.net/21.15107/rcub_imagine_1748 .

TY  - CONF
AU  - Milovanović, Jelena
AU  - Ilić, Bojan
AU  - Radulović, Niko
AU  - Mladenović, Marko
AU  - Makarov, Slobodan
AU  - Divac Rankov, Aleksandra
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1731
AB  - Najveći broj stonoga iz klase Diplopoda poseduje hemijsku zaštitu od predatora
i/ili patogenih mikroorganizama, koji podrazumeva prisustvo odbrambenih
žlezda (ozadena) na trupu čiji se sekreti izbacuju u spoljašnju sredinu preko
otvora koji se naziva ozopora. Predstavnici reda Julida su poznati po tome da su
najčešće dominantne komponente njihovih odbrambenih sekreta hinoni. Pored
hinona, u sekretima ozadena Julida registrovani su i alkoholi, aldehidi, ketoni,
fenolna jedinjenja, kao i brojni estri zasićenih i nezasićenih karboksilnih
kiselina. Dosadašnja istraživanja su pokazala da su ekstrakti odbrambenih
sekreta Julida, kao i pojedinačna jedinjenja koja u njihov sastav ulaze, biološki
aktivni prirodni proizvodi, ali njihov citotoksični potencijal nije dovoljno
istražen. U ovoj studiji je ispitivan uticaj različitih estara linoleinske
kiseline (butil-, pentil-, heksil-, heptil-, oktil-, nonil-, fenetil- i 3-
fenilpropil-linoleat) detektovanih u odbrambenim sekretima stonoga
Megaphyllum bosniense i M. unilineatum na vijabilnost normalnih (BEAS-2B) i
kancerskih (A549) ćelija pluća korišćenjem MTT testa. Svi ispitivani estri su
smanjivali vijabilnost ćelija, pri čemu je postojala značajna razlika u odgovoru
kancerskih u odnosu na normalne ćelije u slučaju tri estra (heksil-, fenetil- i 3-
fenilpropil-linoleat). Iako su estri karboksilnih kiselina poznati kao malo
reaktivna jedinjenja, rezultati ove studije pokazuju da predstavnici ove klase
hemijskih jedinjenja mogu imati citotoksični potencijal.
AB  - Највећи број стонога из класе Diplopoda поседује хемијску заштиту од предатора
и/или патогених микроорганизама, који подразумева присуство одбрамбених
жлезда (озадена) на трупу чији се секрети избацују у спољашњу средину преко
отвора који се назива озопора. Представници реда Julida су познати по томе да су
најчешће доминантне компоненте њихових одбрамбених секрета хинони. Поред
хинона, у секретима озадена Julida регистровани су и алкохоли, алдехиди, кетони,
фенолна једињења, као и бројни естри засићених и незасићених карбоксилних
киселина. Досадашња истраживања су показала да су екстракти одбрамбених
секрета Julida, као и појединачна једињења која у њихов састав улазе, биолошки
активни природни производи, али њихов цитотоксични потенцијал није довољно
истражен. У овој студији је испитиван утицај различитих естара линолеинске
киселине (бутил-, пентил-, хексил-, хептил-, октил-, нонил-, фенетил- и 3-
фенилпропил-линолеат) детектованих у одбрамбеним секретима стонога
Megaphyllum bosniense и M. unilineatum на вијабилност нормалних (BEAS-2B) и
канцeрских (A549) ћелија плућа коришћењем МТТ теста. Сви испитивани естри су
смањивали вијабилност ћелија, при чему је постојала значајна разлика у одговору
канцерских у односу на нормалне ћелије у случају три естра (хексил-, фенетил- и 3-
фенилпропил-линолеат). Иако су естри карбоксилних киселина познати као мало
реактивна једињења, резултати ове студије показују да представници ове класе
хемијских једињења могу имати цитотоксични потенцијал.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida)
T1  - Цитотоксични потенцијал естара линолеинске киселине детектованих у одбрамбеним секретима стонога Megaphyllum bosniense и M. unilineatum (Diplopoda: Julida)
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1731
ER  - 

@conference{
author = "Milovanović, Jelena and Ilić, Bojan and Radulović, Niko and Mladenović, Marko and Makarov, Slobodan and Divac Rankov, Aleksandra",
year = "2022",
abstract = "Najveći broj stonoga iz klase Diplopoda poseduje hemijsku zaštitu od predatora
i/ili patogenih mikroorganizama, koji podrazumeva prisustvo odbrambenih
žlezda (ozadena) na trupu čiji se sekreti izbacuju u spoljašnju sredinu preko
otvora koji se naziva ozopora. Predstavnici reda Julida su poznati po tome da su
najčešće dominantne komponente njihovih odbrambenih sekreta hinoni. Pored
hinona, u sekretima ozadena Julida registrovani su i alkoholi, aldehidi, ketoni,
fenolna jedinjenja, kao i brojni estri zasićenih i nezasićenih karboksilnih
kiselina. Dosadašnja istraživanja su pokazala da su ekstrakti odbrambenih
sekreta Julida, kao i pojedinačna jedinjenja koja u njihov sastav ulaze, biološki
aktivni prirodni proizvodi, ali njihov citotoksični potencijal nije dovoljno
istražen. U ovoj studiji je ispitivan uticaj različitih estara linoleinske
kiseline (butil-, pentil-, heksil-, heptil-, oktil-, nonil-, fenetil- i 3-
fenilpropil-linoleat) detektovanih u odbrambenim sekretima stonoga
Megaphyllum bosniense i M. unilineatum na vijabilnost normalnih (BEAS-2B) i
kancerskih (A549) ćelija pluća korišćenjem MTT testa. Svi ispitivani estri su
smanjivali vijabilnost ćelija, pri čemu je postojala značajna razlika u odgovoru
kancerskih u odnosu na normalne ćelije u slučaju tri estra (heksil-, fenetil- i 3-
fenilpropil-linoleat). Iako su estri karboksilnih kiselina poznati kao malo
reaktivna jedinjenja, rezultati ove studije pokazuju da predstavnici ove klase
hemijskih jedinjenja mogu imati citotoksični potencijal., Највећи број стонога из класе Diplopoda поседује хемијску заштиту од предатора
и/или патогених микроорганизама, који подразумева присуство одбрамбених
жлезда (озадена) на трупу чији се секрети избацују у спољашњу средину преко
отвора који се назива озопора. Представници реда Julida су познати по томе да су
најчешће доминантне компоненте њихових одбрамбених секрета хинони. Поред
хинона, у секретима озадена Julida регистровани су и алкохоли, алдехиди, кетони,
фенолна једињења, као и бројни естри засићених и незасићених карбоксилних
киселина. Досадашња истраживања су показала да су екстракти одбрамбених
секрета Julida, као и појединачна једињења која у њихов састав улазе, биолошки
активни природни производи, али њихов цитотоксични потенцијал није довољно
истражен. У овој студији је испитиван утицај различитих естара линолеинске
киселине (бутил-, пентил-, хексил-, хептил-, октил-, нонил-, фенетил- и 3-
фенилпропил-линолеат) детектованих у одбрамбеним секретима стонога
Megaphyllum bosniense и M. unilineatum на вијабилност нормалних (BEAS-2B) и
канцeрских (A549) ћелија плућа коришћењем МТТ теста. Сви испитивани естри су
смањивали вијабилност ћелија, при чему је постојала значајна разлика у одговору
канцерских у односу на нормалне ћелије у случају три естра (хексил-, фенетил- и 3-
фенилпропил-линолеат). Иако су естри карбоксилних киселина познати као мало
реактивна једињења, резултати ове студије показују да представници ове класе
хемијских једињења могу имати цитотоксични потенцијал.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida), Цитотоксични потенцијал естара линолеинске киселине детектованих у одбрамбеним секретима стонога Megaphyllum bosniense и M. unilineatum (Diplopoda: Julida)",
pages = "186",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1731"
}

Milovanović, J., Ilić, B., Radulović, N., Mladenović, M., Makarov, S.,& Divac Rankov, A.. (2022). Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida). in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 186.
https://hdl.handle.net/21.15107/rcub_imagine_1731

Milovanović J, Ilić B, Radulović N, Mladenović M, Makarov S, Divac Rankov A. Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida). in Treći kongres biologa Srbije. 2022;:186.
https://hdl.handle.net/21.15107/rcub_imagine_1731 .

Milovanović, Jelena, Ilić, Bojan, Radulović, Niko, Mladenović, Marko, Makarov, Slobodan, Divac Rankov, Aleksandra, "Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida)" in Treći kongres biologa Srbije (2022):186,
https://hdl.handle.net/21.15107/rcub_imagine_1731 .

TY  - CONF
AU  - Ljujić, Mila
AU  - Ilić, Bojan
AU  - Pavlović, Danica
AU  - Rabasović, Mihajlo
AU  - Divac Rankov, Aleksandra
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1734
AB  - Komplikacije sa zarastanjem rana i formiranje ožiljnog tkiva predstavljaju
globalni zdravstveni i ekonomski problem. Postoji evidentna potreba za
razvijanjem novih, efikasnih terapija koje bi poboljšale proces zarastanja rana i
transformisale ga u regenerativni proces. Alfa-1 antitripsin (AAT) je
multifunkcionalni protein koji učestvuje u različitim procesima značajnim za
zarastanje rana i regeneraciju – proliferaciji, migraciji, apoptozi i
inflamaciji. Zebrica (Danio rerio) predstavlja značajan model za istraživanja
procesa zarastanja rana, jer ima sposobnost potpune regeneracije amputiranog
repnog peraja. Cilj ovog rada je bio da se ispita uticaj proteina AAT na
regeneraciju repnog peraja embriona zebrice. Embrionima zebrice starim 48 sati
amputirano je repno peraje i zatim merena dužina repa posle 48 h pod tretmanom
(2 mg/ml AAT) i bez njega. Analizirana je ekspresija fibronektina 1a i 1b, dok je
formiranje kolagena analizirano korišćenjem drugog harmonika, nelinearnom
mikroskopijom. Pokazano je da je dužina regenerisanog dela repa veća kada su
embrioni tretirani AAT-om. Ekspresija fibronektina 1a je povećana pod
tretmanom. Primećen je trend povećanja dužine i debljine kolagenskih vlakana
pri tretmanu, što dalje podržava rezultate o podsticanju regeneracije pod
delovanjem AAT. Dobijeni rezultati ukazuju na značajan uticaj alfa-1
antitripsina na brzinu regeneracije repa embriona zebrice. S obzorom da je AAT
odobren kao lek postoji i potencijal da se njegova primena proširi i na lečenje
rana kod ljudi.
AB  - Компликације са зарастањем рана и формирање ожиљног ткива представљају
глобални здравствени и економски проблем. Постоји евидентна потреба за
развијањем нових, ефикасних терапија које би побољшале процес зарастања рана и
трансформисале га у регенеративни процес. Алфа-1 антитрипсин (ААТ) је
мултифункционални протеин који учествује у различитим процесима значајним за
зарастањe рана и регенерацију – пролиферацији, миграцији, апоптози и
инфламацији. Зебрица (Danio rerio) представља значајан модел за истраживања
процеса зарастања рана, јер има способност потпуне регенерације ампутираног
репног пераја. Циљ овог рада је био да се испита утицај протеина ААТ на
регенерацију репног пераја ембриона зебрице. Ембрионима зебрице старим 48 сати
ампутирано је репно пераје и затим мерена дужина репа после 48 h под третманом
(2 мг/мл ААТ) и без њега. Aнализирана је експресија фибронектина 1а и 1b, док је
формирање колагена анализирано коришћењем другог хармоника, нелинеарном
микроскопијом. Показано је да је дужина регенерисаног дела репа већа када су
ембриони третирани ААТ-ом. Експресија фибронектина 1а је повећана под
третманом. Примећен је тренд повећања дужине и дебљине колагенских влакана
при третману, што даље подржава резултате о подстицању регенерације под
деловањем ААТ. Добијени резултати указују на значајан утицај алфа-1
антитрипсина на брзину регенерације репа ембриона зебрице. С обзором да је ААТ
одобрен као лек постоји и потенцијал да се његова примена прошири и на лечење
рана код људи.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Alfa-1 antitripsin podstiče regenerciju repnog peraja embriona zebrice (Danio rerio)
T1  - Алфа-1 антитрипсин подстиче регенерцију репног пераја ембриона зебрице (Danio rerio)
SP  - 314
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1734
ER  - 

@conference{
author = "Ljujić, Mila and Ilić, Bojan and Pavlović, Danica and Rabasović, Mihajlo and Divac Rankov, Aleksandra",
year = "2022",
abstract = "Komplikacije sa zarastanjem rana i formiranje ožiljnog tkiva predstavljaju
globalni zdravstveni i ekonomski problem. Postoji evidentna potreba za
razvijanjem novih, efikasnih terapija koje bi poboljšale proces zarastanja rana i
transformisale ga u regenerativni proces. Alfa-1 antitripsin (AAT) je
multifunkcionalni protein koji učestvuje u različitim procesima značajnim za
zarastanje rana i regeneraciju – proliferaciji, migraciji, apoptozi i
inflamaciji. Zebrica (Danio rerio) predstavlja značajan model za istraživanja
procesa zarastanja rana, jer ima sposobnost potpune regeneracije amputiranog
repnog peraja. Cilj ovog rada je bio da se ispita uticaj proteina AAT na
regeneraciju repnog peraja embriona zebrice. Embrionima zebrice starim 48 sati
amputirano je repno peraje i zatim merena dužina repa posle 48 h pod tretmanom
(2 mg/ml AAT) i bez njega. Analizirana je ekspresija fibronektina 1a i 1b, dok je
formiranje kolagena analizirano korišćenjem drugog harmonika, nelinearnom
mikroskopijom. Pokazano je da je dužina regenerisanog dela repa veća kada su
embrioni tretirani AAT-om. Ekspresija fibronektina 1a je povećana pod
tretmanom. Primećen je trend povećanja dužine i debljine kolagenskih vlakana
pri tretmanu, što dalje podržava rezultate o podsticanju regeneracije pod
delovanjem AAT. Dobijeni rezultati ukazuju na značajan uticaj alfa-1
antitripsina na brzinu regeneracije repa embriona zebrice. S obzorom da je AAT
odobren kao lek postoji i potencijal da se njegova primena proširi i na lečenje
rana kod ljudi., Компликације са зарастањем рана и формирање ожиљног ткива представљају
глобални здравствени и економски проблем. Постоји евидентна потреба за
развијањем нових, ефикасних терапија које би побољшале процес зарастања рана и
трансформисале га у регенеративни процес. Алфа-1 антитрипсин (ААТ) је
мултифункционални протеин који учествује у различитим процесима значајним за
зарастањe рана и регенерацију – пролиферацији, миграцији, апоптози и
инфламацији. Зебрица (Danio rerio) представља значајан модел за истраживања
процеса зарастања рана, јер има способност потпуне регенерације ампутираног
репног пераја. Циљ овог рада је био да се испита утицај протеина ААТ на
регенерацију репног пераја ембриона зебрице. Ембрионима зебрице старим 48 сати
ампутирано је репно пераје и затим мерена дужина репа после 48 h под третманом
(2 мг/мл ААТ) и без њега. Aнализирана је експресија фибронектина 1а и 1b, док је
формирање колагена анализирано коришћењем другог хармоника, нелинеарном
микроскопијом. Показано је да је дужина регенерисаног дела репа већа када су
ембриони третирани ААТ-ом. Експресија фибронектина 1а је повећана под
третманом. Примећен је тренд повећања дужине и дебљине колагенских влакана
при третману, што даље подржава резултате о подстицању регенерације под
деловањем ААТ. Добијени резултати указују на значајан утицај алфа-1
антитрипсина на брзину регенерације репа ембриона зебрице. С обзором да је ААТ
одобрен као лек постоји и потенцијал да се његова примена прошири и на лечење
рана код људи.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Alfa-1 antitripsin podstiče regenerciju repnog peraja embriona zebrice (Danio rerio), Алфа-1 антитрипсин подстиче регенерцију репног пераја ембриона зебрице (Danio rerio)",
pages = "314",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1734"
}

Ljujić, M., Ilić, B., Pavlović, D., Rabasović, M.,& Divac Rankov, A.. (2022). Alfa-1 antitripsin podstiče regenerciju repnog peraja embriona zebrice (Danio rerio). in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 314.
https://hdl.handle.net/21.15107/rcub_imagine_1734

Ljujić M, Ilić B, Pavlović D, Rabasović M, Divac Rankov A. Alfa-1 antitripsin podstiče regenerciju repnog peraja embriona zebrice (Danio rerio). in Treći kongres biologa Srbije. 2022;:314.
https://hdl.handle.net/21.15107/rcub_imagine_1734 .

Ljujić, Mila, Ilić, Bojan, Pavlović, Danica, Rabasović, Mihajlo, Divac Rankov, Aleksandra, "Alfa-1 antitripsin podstiče regenerciju repnog peraja embriona zebrice (Danio rerio)" in Treći kongres biologa Srbije (2022):314,
https://hdl.handle.net/21.15107/rcub_imagine_1734 .

TY  - JOUR
AU  - Trifunović, Sara
AU  - Smiljanić, Katarina
AU  - Sickmann, Albert
AU  - Solari, Fiorella A.
AU  - Kolarević, Stoimir
AU  - Divac Rankov, Aleksandra
AU  - Ljujić, Mila
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1583
AB  - Background Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. Methods In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. Results E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data are available via ProteomeXchange with identifier PXD032071. Conclusions Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.
PB  - BMC, London
T2  - Respiratory Research
T1  - Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells
IS  - 1
VL  - 23
DO  - 10.1186/s12931-022-02102-w
ER  - 

@article{
author = "Trifunović, Sara and Smiljanić, Katarina and Sickmann, Albert and Solari, Fiorella A. and Kolarević, Stoimir and Divac Rankov, Aleksandra and Ljujić, Mila",
year = "2022",
abstract = "Background Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. Methods In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. Results E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data are available via ProteomeXchange with identifier PXD032071. Conclusions Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.",
publisher = "BMC, London",
journal = "Respiratory Research",
title = "Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells",
number = "1",
volume = "23",
doi = "10.1186/s12931-022-02102-w"
}

Trifunović, S., Smiljanić, K., Sickmann, A., Solari, F. A., Kolarević, S., Divac Rankov, A.,& Ljujić, M.. (2022). Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells. in Respiratory Research
BMC, London., 23(1).
https://doi.org/10.1186/s12931-022-02102-w

Trifunović S, Smiljanić K, Sickmann A, Solari FA, Kolarević S, Divac Rankov A, Ljujić M. Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells. in Respiratory Research. 2022;23(1).
doi:10.1186/s12931-022-02102-w .

Trifunović, Sara, Smiljanić, Katarina, Sickmann, Albert, Solari, Fiorella A., Kolarević, Stoimir, Divac Rankov, Aleksandra, Ljujić, Mila, "Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells" in Respiratory Research, 23, no. 1 (2022),
https://doi.org/10.1186/s12931-022-02102-w . .

TY  - JOUR
AU  - Alov, Petko
AU  - Al Sharif, Merilin
AU  - Aluani, Denitsa
AU  - Chegaev, Konstantin
AU  - Dinić, Jelena
AU  - Divac Rankov, Aleksandra
AU  - Fernandes, Miguel X.
AU  - Fusi, Fabio
AU  - Garcia-Sosa, Alfonso T.
AU  - Juvonen, Risto
AU  - Kondeva-Burdina, Magdalena
AU  - Padron, Jose M.
AU  - Pajeva, Ilza
AU  - Pencheva, Tania
AU  - Puerta, Adrian
AU  - Raunio, Hannu
AU  - Riganti, Chiara
AU  - Tsakovska, Ivanka
AU  - Tzankova, Virginia
AU  - Yordanov, Yordan
AU  - Saponara, Simona
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1555
AB  - Sdox is a hydrogen sulfide (H2S)-releasing doxorubicin effective in P-glycoprotein-overexpressing/doxorubicin-resistant tumor models and not cytotoxic, as the parental drug, in H9c2 cardiomyocytes. The aim of this study was the assessment of Sdox drug-like features and its absorption, distribution, metabolism, and excretion (ADME)/toxicity properties, by a multi- and transdisciplinary in silico, in vitro, and in vivo approach. Doxorubicin was used as the reference compound. The in silico profiling suggested that Sdox possesses higher lipophilicity and lower solubility compared to doxorubicin, and the off-targets prediction revealed relevant differences between Dox and Sdox towards several cancer targets, suggesting different toxicological profiles. In vitro data showed that Sdox is a substrate with lower affinity for P-glycoprotein, less hepatotoxic, and causes less oxidative damage than doxorubicin. Both anthracyclines inhibited CYP3A4, but not hERG currents. Unlike doxorubicin, the percentage of zebrafish live embryos at 72 hpf was not affected by Sdox treatment. In conclusion, these findings demonstrate that Sdox displays a more favorable drug-like ADME/toxicity profile than doxorubicin, different selectivity towards cancer targets, along with a greater preclinical efficacy in resistant tumors. Therefore, Sdox represents a prototype of innovative anthracyclines, worthy of further investigations in clinical settings.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Pharmacology
T1  - A Comprehensive Evaluation of Sdox, a Promising H2S-Releasing Doxorubicin for the Treatment of Chemoresistant Tumors
VL  - 13
DO  - 10.3389/fphar.2022.831791
ER  - 

@article{
author = "Alov, Petko and Al Sharif, Merilin and Aluani, Denitsa and Chegaev, Konstantin and Dinić, Jelena and Divac Rankov, Aleksandra and Fernandes, Miguel X. and Fusi, Fabio and Garcia-Sosa, Alfonso T. and Juvonen, Risto and Kondeva-Burdina, Magdalena and Padron, Jose M. and Pajeva, Ilza and Pencheva, Tania and Puerta, Adrian and Raunio, Hannu and Riganti, Chiara and Tsakovska, Ivanka and Tzankova, Virginia and Yordanov, Yordan and Saponara, Simona",
year = "2022",
abstract = "Sdox is a hydrogen sulfide (H2S)-releasing doxorubicin effective in P-glycoprotein-overexpressing/doxorubicin-resistant tumor models and not cytotoxic, as the parental drug, in H9c2 cardiomyocytes. The aim of this study was the assessment of Sdox drug-like features and its absorption, distribution, metabolism, and excretion (ADME)/toxicity properties, by a multi- and transdisciplinary in silico, in vitro, and in vivo approach. Doxorubicin was used as the reference compound. The in silico profiling suggested that Sdox possesses higher lipophilicity and lower solubility compared to doxorubicin, and the off-targets prediction revealed relevant differences between Dox and Sdox towards several cancer targets, suggesting different toxicological profiles. In vitro data showed that Sdox is a substrate with lower affinity for P-glycoprotein, less hepatotoxic, and causes less oxidative damage than doxorubicin. Both anthracyclines inhibited CYP3A4, but not hERG currents. Unlike doxorubicin, the percentage of zebrafish live embryos at 72 hpf was not affected by Sdox treatment. In conclusion, these findings demonstrate that Sdox displays a more favorable drug-like ADME/toxicity profile than doxorubicin, different selectivity towards cancer targets, along with a greater preclinical efficacy in resistant tumors. Therefore, Sdox represents a prototype of innovative anthracyclines, worthy of further investigations in clinical settings.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Pharmacology",
title = "A Comprehensive Evaluation of Sdox, a Promising H2S-Releasing Doxorubicin for the Treatment of Chemoresistant Tumors",
volume = "13",
doi = "10.3389/fphar.2022.831791"
}

Alov, P., Al Sharif, M., Aluani, D., Chegaev, K., Dinić, J., Divac Rankov, A., Fernandes, M. X., Fusi, F., Garcia-Sosa, A. T., Juvonen, R., Kondeva-Burdina, M., Padron, J. M., Pajeva, I., Pencheva, T., Puerta, A., Raunio, H., Riganti, C., Tsakovska, I., Tzankova, V., Yordanov, Y.,& Saponara, S.. (2022). A Comprehensive Evaluation of Sdox, a Promising H2S-Releasing Doxorubicin for the Treatment of Chemoresistant Tumors. in Frontiers in Pharmacology
Frontiers Media Sa, Lausanne., 13.
https://doi.org/10.3389/fphar.2022.831791

Alov P, Al Sharif M, Aluani D, Chegaev K, Dinić J, Divac Rankov A, Fernandes MX, Fusi F, Garcia-Sosa AT, Juvonen R, Kondeva-Burdina M, Padron JM, Pajeva I, Pencheva T, Puerta A, Raunio H, Riganti C, Tsakovska I, Tzankova V, Yordanov Y, Saponara S. A Comprehensive Evaluation of Sdox, a Promising H2S-Releasing Doxorubicin for the Treatment of Chemoresistant Tumors. in Frontiers in Pharmacology. 2022;13.
doi:10.3389/fphar.2022.831791 .

Alov, Petko, Al Sharif, Merilin, Aluani, Denitsa, Chegaev, Konstantin, Dinić, Jelena, Divac Rankov, Aleksandra, Fernandes, Miguel X., Fusi, Fabio, Garcia-Sosa, Alfonso T., Juvonen, Risto, Kondeva-Burdina, Magdalena, Padron, Jose M., Pajeva, Ilza, Pencheva, Tania, Puerta, Adrian, Raunio, Hannu, Riganti, Chiara, Tsakovska, Ivanka, Tzankova, Virginia, Yordanov, Yordan, Saponara, Simona, "A Comprehensive Evaluation of Sdox, a Promising H2S-Releasing Doxorubicin for the Treatment of Chemoresistant Tumors" in Frontiers in Pharmacology, 13 (2022),
https://doi.org/10.3389/fphar.2022.831791 . .

TY  - CONF
AU  - Milovanović, Jelena
AU  - Divac Rankov, Aleksandra
AU  - Radulović, Niko
AU  - Mladenović, Marko
AU  - Ilić, Bojan
AU  - Makarov, Slobodan
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1433
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - The effect of new natural long-chain fatty acid esters from millipede defensive secretion on reactive oxygen species (ROS) production in a cell-free model system
EP  - S39
SP  - S39
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.08.119
ER  - 

@conference{
author = "Milovanović, Jelena and Divac Rankov, Aleksandra and Radulović, Niko and Mladenović, Marko and Ilić, Bojan and Makarov, Slobodan",
year = "2021",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "The effect of new natural long-chain fatty acid esters from millipede defensive secretion on reactive oxygen species (ROS) production in a cell-free model system",
pages = "S39-S39",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.08.119"
}

Milovanović, J., Divac Rankov, A., Radulović, N., Mladenović, M., Ilić, B.,& Makarov, S.. (2021). The effect of new natural long-chain fatty acid esters from millipede defensive secretion on reactive oxygen species (ROS) production in a cell-free model system. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 177, S39-S39.
https://doi.org/10.1016/j.freeradbiomed.2021.08.119

Milovanović J, Divac Rankov A, Radulović N, Mladenović M, Ilić B, Makarov S. The effect of new natural long-chain fatty acid esters from millipede defensive secretion on reactive oxygen species (ROS) production in a cell-free model system. in Free Radical Biology and Medicine. 2021;177:S39-S39.
doi:10.1016/j.freeradbiomed.2021.08.119 .

Milovanović, Jelena, Divac Rankov, Aleksandra, Radulović, Niko, Mladenović, Marko, Ilić, Bojan, Makarov, Slobodan, "The effect of new natural long-chain fatty acid esters from millipede defensive secretion on reactive oxygen species (ROS) production in a cell-free model system" in Free Radical Biology and Medicine, 177 (2021):S39-S39,
https://doi.org/10.1016/j.freeradbiomed.2021.08.119 . .

TY  - JOUR
AU  - Nesović, Marija
AU  - Divac Rankov, Aleksandra
AU  - Podolski-Renić, Ana
AU  - Nikolić, Igor
AU  - Tasić, Goran
AU  - Mancini, Arianna
AU  - Schenone, Silvia
AU  - Pesić, Milica
AU  - Dinić, Jelena
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1315
AB  - Glioblastoma (GBM), as the most aggressive brain tumor, displays a high expression of Src tyrosine kinase, which is involved in the survival, migration, and invasiveness of tumor cells. Thus, Src emerged as a potential target for GBM therapy. The effects of Src inhibitors pyrazolo[3,4-d]pyrimidines, Si306 and its prodrug pro-Si306 were investigated in human GBM cell lines (U87 and U87-TxR) and three primary GBM cell cultures. Primary GBM cells were more resistant to Si306 and pro-Si306 according to the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. However, the ability of all GBM cells to degrade the extracellular matrix was considerably compromised after Si306 and pro-Si306 applications. Besides reducing the phosphorylation of Src and its downstream signaling pathway components, both compounds decreased the phosphorylated form of focal adhesion kinase (FAK) and epidermal growth factor receptor (EGFR) expression, showing the potential to suppress the aggressiveness of GBM. In vivo, Si306 and pro-Si306 displayed an anti-invasive effect against U87 xenografts in the zebrafish embryo model. Considering that Si306 and pro-Si306 are able to cross the blood-brain barrier and suppress the spread of GBM cells, we anticipate their clinical testing in the near future. Moreover, the prodrug showed similar efficacy to the drug, implying the rationality of its use in clinical settings.
PB  - MDPI, Basel
T2  - Cancers
T1  - Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo
IS  - 6
VL  - 12
DO  - 10.3390/cancers12061570
ER  - 

@article{
author = "Nesović, Marija and Divac Rankov, Aleksandra and Podolski-Renić, Ana and Nikolić, Igor and Tasić, Goran and Mancini, Arianna and Schenone, Silvia and Pesić, Milica and Dinić, Jelena",
year = "2020",
abstract = "Glioblastoma (GBM), as the most aggressive brain tumor, displays a high expression of Src tyrosine kinase, which is involved in the survival, migration, and invasiveness of tumor cells. Thus, Src emerged as a potential target for GBM therapy. The effects of Src inhibitors pyrazolo[3,4-d]pyrimidines, Si306 and its prodrug pro-Si306 were investigated in human GBM cell lines (U87 and U87-TxR) and three primary GBM cell cultures. Primary GBM cells were more resistant to Si306 and pro-Si306 according to the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. However, the ability of all GBM cells to degrade the extracellular matrix was considerably compromised after Si306 and pro-Si306 applications. Besides reducing the phosphorylation of Src and its downstream signaling pathway components, both compounds decreased the phosphorylated form of focal adhesion kinase (FAK) and epidermal growth factor receptor (EGFR) expression, showing the potential to suppress the aggressiveness of GBM. In vivo, Si306 and pro-Si306 displayed an anti-invasive effect against U87 xenografts in the zebrafish embryo model. Considering that Si306 and pro-Si306 are able to cross the blood-brain barrier and suppress the spread of GBM cells, we anticipate their clinical testing in the near future. Moreover, the prodrug showed similar efficacy to the drug, implying the rationality of its use in clinical settings.",
publisher = "MDPI, Basel",
journal = "Cancers",
title = "Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo",
number = "6",
volume = "12",
doi = "10.3390/cancers12061570"
}

Nesović, M., Divac Rankov, A., Podolski-Renić, A., Nikolić, I., Tasić, G., Mancini, A., Schenone, S., Pesić, M.,& Dinić, J.. (2020). Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo. in Cancers
MDPI, Basel., 12(6).
https://doi.org/10.3390/cancers12061570

Nesović M, Divac Rankov A, Podolski-Renić A, Nikolić I, Tasić G, Mancini A, Schenone S, Pesić M, Dinić J. Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo. in Cancers. 2020;12(6).
doi:10.3390/cancers12061570 .

Nesović, Marija, Divac Rankov, Aleksandra, Podolski-Renić, Ana, Nikolić, Igor, Tasić, Goran, Mancini, Arianna, Schenone, Silvia, Pesić, Milica, Dinić, Jelena, "Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo" in Cancers, 12, no. 6 (2020),
https://doi.org/10.3390/cancers12061570 . .

TY  - CHAP
AU  - Divac Rankov, Aleksandra
AU  - Ljujić, Mila
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1637
AB  - Estimated human genetic diversity is 0.1%, meaning that any two individuals differ at approximately one in every thousand base pairs. A small proportion of these genetic variants have functional consequences affecting health, including susceptibility to infections, diseases, and response to different drugs. Advancements in high-throughput sequencing technology have provided more insight into human genetic makeup and enabled better understanding of the link between genetic variations and health outcomes. However, despite considerable progress in human genetics, there are still many diseases that, given their complexity, cannot be explained simply by the genetic variants present and the involvement of factors such as epigenetics, environmental factors, and gene–environment interactions must be considered. We will discuss how human genetic diversity helps us to uncover the underlining mechanisms in monogenic, complex, and infectious diseases leading to a personalized approach to drug development and treatment.
PB  - Academic Press
T2  - Biodiversity and Biomedicine
T1  - Chapter 8 - Human genetic diversity in health and disease
EP  - 136
SP  - 123
DO  - 10.1016/B978-0-12-819541-3.00008-6
ER  - 

@inbook{
author = "Divac Rankov, Aleksandra and Ljujić, Mila",
year = "2020",
abstract = "Estimated human genetic diversity is 0.1%, meaning that any two individuals differ at approximately one in every thousand base pairs. A small proportion of these genetic variants have functional consequences affecting health, including susceptibility to infections, diseases, and response to different drugs. Advancements in high-throughput sequencing technology have provided more insight into human genetic makeup and enabled better understanding of the link between genetic variations and health outcomes. However, despite considerable progress in human genetics, there are still many diseases that, given their complexity, cannot be explained simply by the genetic variants present and the involvement of factors such as epigenetics, environmental factors, and gene–environment interactions must be considered. We will discuss how human genetic diversity helps us to uncover the underlining mechanisms in monogenic, complex, and infectious diseases leading to a personalized approach to drug development and treatment.",
publisher = "Academic Press",
journal = "Biodiversity and Biomedicine",
booktitle = "Chapter 8 - Human genetic diversity in health and disease",
pages = "136-123",
doi = "10.1016/B978-0-12-819541-3.00008-6"
}

Divac Rankov, A.,& Ljujić, M.. (2020). Chapter 8 - Human genetic diversity in health and disease. in Biodiversity and Biomedicine
Academic Press., 123-136.
https://doi.org/10.1016/B978-0-12-819541-3.00008-6

Divac Rankov A, Ljujić M. Chapter 8 - Human genetic diversity in health and disease. in Biodiversity and Biomedicine. 2020;:123-136.
doi:10.1016/B978-0-12-819541-3.00008-6 .

Divac Rankov, Aleksandra, Ljujić, Mila, "Chapter 8 - Human genetic diversity in health and disease" in Biodiversity and Biomedicine (2020):123-136,
https://doi.org/10.1016/B978-0-12-819541-3.00008-6 . .

TY  - JOUR
AU  - Divac Rankov, Aleksandra
AU  - Kušić-Tišma, Jelena
AU  - Ljujić, Mila
AU  - Nikolić, Aleksandra
AU  - Milosević, Katarina
AU  - Dautović, Gordana Vilotijevic
AU  - Radojković, Dragica
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1381
AB  - Background: High heterogeneity levels of cystic fibrosis transmembrane regulator (CFTR) are manifested in different populations. The aim of this study was to analyze comprehensively all mutations in the CFTR gene in Serbian patients with cystic fibrosis (CF) and to use the findings to propose a testing algorithm for the Serbian population. Materials and Methods: Cascade screening was employed to detect mutations in the CFTR gene of 90 patients suspected of having CF, using polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism or PCR-mediated site directed mutagenesis, Sanger sequencing, and/or next-generation sequencing. Results: This is the first report for the Serbian CF population where single nucleotide polymorphisms, small insertions and deletions, large genome rearrangements, and copy number variants were analyzed in detail. A high degree of heterogeneity within the CFTR was documented among our cohort of 90 patients. We identified 19 CF-causing mutations and 3 with varying consequences, including a previously unreported deletion of the entire exon 11. Conclusion: Considering the spectrum and frequency of mutations found, we recommend a multistep sequencing algorithm in combination with evaluation of large rearrangements for future analyses of the CFTR gene in the Serbian population.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Genetic Testing and Molecular Biomarkers
T1  - Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges
EP  - 216
IS  - 4
SP  - 212
VL  - 24
DO  - 10.1089/gtmb.2019.0171
ER  - 

@article{
author = "Divac Rankov, Aleksandra and Kušić-Tišma, Jelena and Ljujić, Mila and Nikolić, Aleksandra and Milosević, Katarina and Dautović, Gordana Vilotijevic and Radojković, Dragica",
year = "2020",
abstract = "Background: High heterogeneity levels of cystic fibrosis transmembrane regulator (CFTR) are manifested in different populations. The aim of this study was to analyze comprehensively all mutations in the CFTR gene in Serbian patients with cystic fibrosis (CF) and to use the findings to propose a testing algorithm for the Serbian population. Materials and Methods: Cascade screening was employed to detect mutations in the CFTR gene of 90 patients suspected of having CF, using polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism or PCR-mediated site directed mutagenesis, Sanger sequencing, and/or next-generation sequencing. Results: This is the first report for the Serbian CF population where single nucleotide polymorphisms, small insertions and deletions, large genome rearrangements, and copy number variants were analyzed in detail. A high degree of heterogeneity within the CFTR was documented among our cohort of 90 patients. We identified 19 CF-causing mutations and 3 with varying consequences, including a previously unreported deletion of the entire exon 11. Conclusion: Considering the spectrum and frequency of mutations found, we recommend a multistep sequencing algorithm in combination with evaluation of large rearrangements for future analyses of the CFTR gene in the Serbian population.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Genetic Testing and Molecular Biomarkers",
title = "Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges",
pages = "216-212",
number = "4",
volume = "24",
doi = "10.1089/gtmb.2019.0171"
}

Divac Rankov, A., Kušić-Tišma, J., Ljujić, M., Nikolić, A., Milosević, K., Dautović, G. V.,& Radojković, D.. (2020). Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges. in Genetic Testing and Molecular Biomarkers
Mary Ann Liebert, Inc, New Rochelle., 24(4), 212-216.
https://doi.org/10.1089/gtmb.2019.0171

Divac Rankov A, Kušić-Tišma J, Ljujić M, Nikolić A, Milosević K, Dautović GV, Radojković D. Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges. in Genetic Testing and Molecular Biomarkers. 2020;24(4):212-216.
doi:10.1089/gtmb.2019.0171 .

Divac Rankov, Aleksandra, Kušić-Tišma, Jelena, Ljujić, Mila, Nikolić, Aleksandra, Milosević, Katarina, Dautović, Gordana Vilotijevic, Radojković, Dragica, "Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges" in Genetic Testing and Molecular Biomarkers, 24, no. 4 (2020):212-216,
https://doi.org/10.1089/gtmb.2019.0171 . .

TY  - CONF
AU  - Divac Rankov, Aleksandra
AU  - Trifunović, Sara
AU  - Ljujić, Mila
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1334
PB  - European Respiratory Soc Journals Ltd, Sheffield
C3  - European Respiratory Journal
T1  - Electronic cigarette liquids induce formation of tunnelling nanotubes in BEAS2B cells
VL  - 56
DO  - 10.1183/13993003.congress-2020.1967
ER  - 

@conference{
author = "Divac Rankov, Aleksandra and Trifunović, Sara and Ljujić, Mila",
year = "2020",
publisher = "European Respiratory Soc Journals Ltd, Sheffield",
journal = "European Respiratory Journal",
title = "Electronic cigarette liquids induce formation of tunnelling nanotubes in BEAS2B cells",
volume = "56",
doi = "10.1183/13993003.congress-2020.1967"
}

Divac Rankov, A., Trifunović, S.,& Ljujić, M.. (2020). Electronic cigarette liquids induce formation of tunnelling nanotubes in BEAS2B cells. in European Respiratory Journal
European Respiratory Soc Journals Ltd, Sheffield., 56.
https://doi.org/10.1183/13993003.congress-2020.1967

Divac Rankov A, Trifunović S, Ljujić M. Electronic cigarette liquids induce formation of tunnelling nanotubes in BEAS2B cells. in European Respiratory Journal. 2020;56.
doi:10.1183/13993003.congress-2020.1967 .

Divac Rankov, Aleksandra, Trifunović, Sara, Ljujić, Mila, "Electronic cigarette liquids induce formation of tunnelling nanotubes in BEAS2B cells" in European Respiratory Journal, 56 (2020),
https://doi.org/10.1183/13993003.congress-2020.1967 . .

TY  - CHAP
AU  - Kračun-Kolarević, M.
AU  - Kolarević, S.
AU  - Jovanović, J.
AU  - Đorđević, J.
AU  - Ilić, M.
AU  - Sunjog, K.
AU  - Kostić-Vuković, J.
AU  - Divac Rankov, Aleksandra
AU  - Ilić, B.
AU  - Pešić, V.
AU  - Vuković-Gačić, B.
AU  - Paunović, M.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1347
AB  - Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Handbook of Environmental Chemistry
T1  - Impact of pollution on rivers in Montenegro: Ecotoxicological perspective
EP  - 133
SP  - 111
VL  - 93
DO  - 10.1007/698_2019_425
ER  - 

@inbook{
author = "Kračun-Kolarević, M. and Kolarević, S. and Jovanović, J. and Đorđević, J. and Ilić, M. and Sunjog, K. and Kostić-Vuković, J. and Divac Rankov, Aleksandra and Ilić, B. and Pešić, V. and Vuković-Gačić, B. and Paunović, M.",
year = "2020",
abstract = "Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Handbook of Environmental Chemistry",
booktitle = "Impact of pollution on rivers in Montenegro: Ecotoxicological perspective",
pages = "133-111",
volume = "93",
doi = "10.1007/698_2019_425"
}

Kračun-Kolarević, M., Kolarević, S., Jovanović, J., Đorđević, J., Ilić, M., Sunjog, K., Kostić-Vuković, J., Divac Rankov, A., Ilić, B., Pešić, V., Vuković-Gačić, B.,& Paunović, M.. (2020). Impact of pollution on rivers in Montenegro: Ecotoxicological perspective. in Handbook of Environmental Chemistry
Springer Science and Business Media Deutschland GmbH., 93, 111-133.
https://doi.org/10.1007/698_2019_425

Kračun-Kolarević M, Kolarević S, Jovanović J, Đorđević J, Ilić M, Sunjog K, Kostić-Vuković J, Divac Rankov A, Ilić B, Pešić V, Vuković-Gačić B, Paunović M. Impact of pollution on rivers in Montenegro: Ecotoxicological perspective. in Handbook of Environmental Chemistry. 2020;93:111-133.
doi:10.1007/698_2019_425 .

Kračun-Kolarević, M., Kolarević, S., Jovanović, J., Đorđević, J., Ilić, M., Sunjog, K., Kostić-Vuković, J., Divac Rankov, Aleksandra, Ilić, B., Pešić, V., Vuković-Gačić, B., Paunović, M., "Impact of pollution on rivers in Montenegro: Ecotoxicological perspective" in Handbook of Environmental Chemistry, 93 (2020):111-133,
https://doi.org/10.1007/698_2019_425 . .

TY  - JOUR
AU  - Dragičević, Sandra
AU  - Kovacević, Draginja
AU  - Divac Rankov, Aleksandra
AU  - Nikolić, Aleksandra
AU  - Radojković, Dragica
AU  - Radović, Svetlana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1233
AB  - Tussilago farfara (coltsfoot) and Verbascum thapsus (mullein) have been used as folk remedies for treating respiratory disorders. The aim of this study was to test the toxicity of the water extracts of T. farfara and V. thapsus in vivo in zebrafish and in vitro in BEAS 2B epithelial bronchial cells. To the best of our knowledge, this is the first study to investigate the antioxidative properties of T. farfara and V. thapsus extracts in cell culture. Our results show that the T. farfara leaf extract does not produce toxic effects on zebrafish embryos or BEAS 2B cells, and that it has a protective effect in BEAS 2B after induction of oxidative stress. The water extract from V. thapsus displayed pronounced toxic effects on zebrafish embryos and BEAS 2B cells and did not exhibit a significant antioxidative effect on BEAS 2B cells exposed to oxidative stress. Our results suggest that the use of T. farfara water leaf extract is potentially safe and effective in treating respiratory disorders, whereas the use of V. thapsus needs further investigation.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells
EP  - 416
IS  - 3
SP  - 409
VL  - 71
DO  - 10.2298/ABS181213022D
ER  - 

@article{
author = "Dragičević, Sandra and Kovacević, Draginja and Divac Rankov, Aleksandra and Nikolić, Aleksandra and Radojković, Dragica and Radović, Svetlana",
year = "2019",
abstract = "Tussilago farfara (coltsfoot) and Verbascum thapsus (mullein) have been used as folk remedies for treating respiratory disorders. The aim of this study was to test the toxicity of the water extracts of T. farfara and V. thapsus in vivo in zebrafish and in vitro in BEAS 2B epithelial bronchial cells. To the best of our knowledge, this is the first study to investigate the antioxidative properties of T. farfara and V. thapsus extracts in cell culture. Our results show that the T. farfara leaf extract does not produce toxic effects on zebrafish embryos or BEAS 2B cells, and that it has a protective effect in BEAS 2B after induction of oxidative stress. The water extract from V. thapsus displayed pronounced toxic effects on zebrafish embryos and BEAS 2B cells and did not exhibit a significant antioxidative effect on BEAS 2B cells exposed to oxidative stress. Our results suggest that the use of T. farfara water leaf extract is potentially safe and effective in treating respiratory disorders, whereas the use of V. thapsus needs further investigation.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells",
pages = "416-409",
number = "3",
volume = "71",
doi = "10.2298/ABS181213022D"
}

Dragičević, S., Kovacević, D., Divac Rankov, A., Nikolić, A., Radojković, D.,& Radović, S.. (2019). Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(3), 409-416.
https://doi.org/10.2298/ABS181213022D

Dragičević S, Kovacević D, Divac Rankov A, Nikolić A, Radojković D, Radović S. Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells. in Archives of Biological Sciences. 2019;71(3):409-416.
doi:10.2298/ABS181213022D .

Dragičević, Sandra, Kovacević, Draginja, Divac Rankov, Aleksandra, Nikolić, Aleksandra, Radojković, Dragica, Radović, Svetlana, "Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells" in Archives of Biological Sciences, 71, no. 3 (2019):409-416,
https://doi.org/10.2298/ABS181213022D . .

TY  - CONF
AU  - Ljujić, Mila
AU  - Vignjević, Nataša
AU  - Divac Rankov, Aleksandra
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1830
AB  - The use of electronic cigarettes is on the rise with a public perceiving them as a safer alternative to conventional cigarettes. However, their safety has only recently started being studied to a greater extent.

The aim of this study was to test the toxicity of commercially available electronic cigarette liquids (ECL) with and without nicotine in vitro and in vivo.

Two cell lines originating from the lung tissue were used - A549 and BEAS-2B. Using MTT assay, we have shown that there is a dose dependant decrease in cell viability in case of both tested liquids, and in both cell lines. We also detected cell-free mitochondrial DNA in culture medium of treated cells, indicating necrotic cell death. In order to investigate if cells undergone necroptosis we used pre-treatment with necrostatin-1 (Nec-1), a specific RIP-1 kinase inhibitor. The addition of Nec-1 did not affect cell survival under tested conditions suggesting that the cells do not go through the process of controlled necrosis.

In vivo toxicity of different concentrations of ECL was assessed by the analysis of survival, hatching and malformations of zebrafish embryos. The effects of ECL with nicotine were comparable to those when embryos were treated with pure nicotine at the same concentration, while the ECL without nicotine did not have toxic effects in used concentrations. The embryo mortality was increased when treated with ECL with 20µg/mL of nicotine and several embryo malformations (pericardial oedema, body curvature, shortening of the body and decreased movements) were present at 10µg/mL concentration.

Our result highlight the need for further testing the molecular effects and safety of electronic cigarettes.
PB  - The European Respiratory Society
C3  - European Respiratory Journal
T1  - Electronic cigarette liquids show toxic effects in vitro and in vivo
SP  - PA2444
VL  - 54
DO  - 10.1183/13993003.congress-2019.PA2444
ER  - 

@conference{
author = "Ljujić, Mila and Vignjević, Nataša and Divac Rankov, Aleksandra",
year = "2019",
abstract = "The use of electronic cigarettes is on the rise with a public perceiving them as a safer alternative to conventional cigarettes. However, their safety has only recently started being studied to a greater extent.

The aim of this study was to test the toxicity of commercially available electronic cigarette liquids (ECL) with and without nicotine in vitro and in vivo.

Two cell lines originating from the lung tissue were used - A549 and BEAS-2B. Using MTT assay, we have shown that there is a dose dependant decrease in cell viability in case of both tested liquids, and in both cell lines. We also detected cell-free mitochondrial DNA in culture medium of treated cells, indicating necrotic cell death. In order to investigate if cells undergone necroptosis we used pre-treatment with necrostatin-1 (Nec-1), a specific RIP-1 kinase inhibitor. The addition of Nec-1 did not affect cell survival under tested conditions suggesting that the cells do not go through the process of controlled necrosis.

In vivo toxicity of different concentrations of ECL was assessed by the analysis of survival, hatching and malformations of zebrafish embryos. The effects of ECL with nicotine were comparable to those when embryos were treated with pure nicotine at the same concentration, while the ECL without nicotine did not have toxic effects in used concentrations. The embryo mortality was increased when treated with ECL with 20µg/mL of nicotine and several embryo malformations (pericardial oedema, body curvature, shortening of the body and decreased movements) were present at 10µg/mL concentration.

Our result highlight the need for further testing the molecular effects and safety of electronic cigarettes.",
publisher = "The European Respiratory Society",
journal = "European Respiratory Journal",
title = "Electronic cigarette liquids show toxic effects in vitro and in vivo",
pages = "PA2444",
volume = "54",
doi = "10.1183/13993003.congress-2019.PA2444"
}

Ljujić, M., Vignjević, N.,& Divac Rankov, A.. (2019). Electronic cigarette liquids show toxic effects in vitro and in vivo. in European Respiratory Journal
The European Respiratory Society., 54, PA2444.
https://doi.org/10.1183/13993003.congress-2019.PA2444

Ljujić M, Vignjević N, Divac Rankov A. Electronic cigarette liquids show toxic effects in vitro and in vivo. in European Respiratory Journal. 2019;54:PA2444.
doi:10.1183/13993003.congress-2019.PA2444 .

Ljujić, Mila, Vignjević, Nataša, Divac Rankov, Aleksandra, "Electronic cigarette liquids show toxic effects in vitro and in vivo" in European Respiratory Journal, 54 (2019):PA2444,
https://doi.org/10.1183/13993003.congress-2019.PA2444 . .

TY  - JOUR
AU  - Babić, Tamara
AU  - Dinić, Jelena
AU  - Stojković Burić, Sonja
AU  - Hadzić, Stefan
AU  - Pesić, Milica
AU  - Radojković, Dragica
AU  - Divac Rankov, Aleksandra
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1132
AB  - Cancer drug resistance and poor selectivity towards cancer cells demand the constant search for new therapeutics. PI3K-Akt-mTOR and RAS-MAPK-ERK signaling pathways are key mechanisms involved in cell survival, proliferation, differentiation, and metabolism and their deregulation in cancer can promote development of therapy resistance. We investigated the effects of targeted inhibitors (wortmannin, GSK690693, AZD2014 and tipifarnib) towards these two pathways on early zebrafish and sea urchin development to assess their toxicity in normal, fast proliferating cells. PI3K inhibitor wortmannin and RAS inhibitor tipifarnib displayed highest toxicity while GSK690693, a pan-Akt kinase inhibitor, exhibited a less significant impact on embryo survival and development. Moreover, inhibition of the upstream part of the PI3K-Akt-mTOR pathway (wortmannin/GSK690693 co-treatment) produced a synergistic effect and impacted zebrafish embryo survival and development at much lower concentrations. Dual mTORC1/mTORC2 inhibitor AZD2014 showed no considerable effects on embryonic cells of zebrafish in concentrations substantially toxic in cancer cells. AZD2014 also caused the least prominent effects on sea urchin embryo development compared to other inhibitors. Significant toxicity of AZD2014 in human cancer cells, its capacity to sensitize resistant cancers, lower antiproliferative activity against human normal cell lines and fast proliferating embryonic cells could make this agent a promising candidate for anticancer therapy.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Biologica Hungarica
T1  - Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models
EP  - 410
IS  - 4
SP  - 395
VL  - 69
DO  - 10.1556/018.69.2018.4.3
ER  - 

@article{
author = "Babić, Tamara and Dinić, Jelena and Stojković Burić, Sonja and Hadzić, Stefan and Pesić, Milica and Radojković, Dragica and Divac Rankov, Aleksandra",
year = "2018",
abstract = "Cancer drug resistance and poor selectivity towards cancer cells demand the constant search for new therapeutics. PI3K-Akt-mTOR and RAS-MAPK-ERK signaling pathways are key mechanisms involved in cell survival, proliferation, differentiation, and metabolism and their deregulation in cancer can promote development of therapy resistance. We investigated the effects of targeted inhibitors (wortmannin, GSK690693, AZD2014 and tipifarnib) towards these two pathways on early zebrafish and sea urchin development to assess their toxicity in normal, fast proliferating cells. PI3K inhibitor wortmannin and RAS inhibitor tipifarnib displayed highest toxicity while GSK690693, a pan-Akt kinase inhibitor, exhibited a less significant impact on embryo survival and development. Moreover, inhibition of the upstream part of the PI3K-Akt-mTOR pathway (wortmannin/GSK690693 co-treatment) produced a synergistic effect and impacted zebrafish embryo survival and development at much lower concentrations. Dual mTORC1/mTORC2 inhibitor AZD2014 showed no considerable effects on embryonic cells of zebrafish in concentrations substantially toxic in cancer cells. AZD2014 also caused the least prominent effects on sea urchin embryo development compared to other inhibitors. Significant toxicity of AZD2014 in human cancer cells, its capacity to sensitize resistant cancers, lower antiproliferative activity against human normal cell lines and fast proliferating embryonic cells could make this agent a promising candidate for anticancer therapy.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Biologica Hungarica",
title = "Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models",
pages = "410-395",
number = "4",
volume = "69",
doi = "10.1556/018.69.2018.4.3"
}

Babić, T., Dinić, J., Stojković Burić, S., Hadzić, S., Pesić, M., Radojković, D.,& Divac Rankov, A.. (2018). Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models. in Acta Biologica Hungarica
Akademiai Kiado Zrt, Budapest., 69(4), 395-410.
https://doi.org/10.1556/018.69.2018.4.3

Babić T, Dinić J, Stojković Burić S, Hadzić S, Pesić M, Radojković D, Divac Rankov A. Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models. in Acta Biologica Hungarica. 2018;69(4):395-410.
doi:10.1556/018.69.2018.4.3 .

Babić, Tamara, Dinić, Jelena, Stojković Burić, Sonja, Hadzić, Stefan, Pesić, Milica, Radojković, Dragica, Divac Rankov, Aleksandra, "Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models" in Acta Biologica Hungarica, 69, no. 4 (2018):395-410,
https://doi.org/10.1556/018.69.2018.4.3 . .

TY  - JOUR
AU  - Hammer, Barbara
AU  - Wagner, Christina
AU  - Divac Rankov, Aleksandra
AU  - Reuter, Sebastian
AU  - Bartel, Sabine
AU  - Hylkema, Machteld N.
AU  - Krueger, Arne
AU  - Svanes, Cecilie
AU  - Krauss-Etschmann, Susanne
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1129
AB  - Background: The prevalence of asthma and chronic obstructive pulmonary disease (COPD) has risen markedly over the last decades and is reaching epidemic proportions. However, underlying molecular mechanisms are not fully understood, hampering the urgently needed development of approaches to prevent these diseases. It is well established from epidemiological studies that prenatal exposure to cigarette smoke is one of the main risk factors for aberrant lung function development or reduced fetal growth, but also for the development of asthma and possibly COPD later in life. Of note, recent evidence suggests that the disease risk can be transferred across generations, that is, from grandparents to their grandchildren. While initial studies in mouse models on in utero smoke exposure have provided important mechanistic insights, there are still knowledge gaps that need to be filled. Objective: Thus, in this review, we summarize current knowledge on this topic derived from mouse models, while also introducing two other relevant animal models: the fruit fly Drosophila melanogaster and the zebrafish Danio rerio. Methods: This review is based on an intensive review of PubMed-listed transgenerational animal studies from 1902 to 2018 and focuses in detail on selected literature due to space limitations. Results: This review gives a comprehensive overview of mechanistic insights obtained in studies with the three species, while highlighting the remaining knowledge gaps. We will further discuss potential (dis)advantages of all three animal models. Conclusion/Clinical Relevance: Many studies have already addressed transgenerational inheritance of disease risk in mouse, zebrafish or fly models. We here propose a novel strategy for how these three model organisms can be synergistically combined to achieve a more detailed understanding of in utero cigarette smoke-induced transgenerational inheritance of disease risk.
PB  - Wiley, Hoboken
T2  - Clinical and Experimental Allergy
T1  - In utero exposure to cigarette smoke and effects across generations: A conference of animals on asthma
EP  - 1390
IS  - 11
SP  - 1378
VL  - 48
DO  - 10.1111/cea.13283
ER  - 

@article{
author = "Hammer, Barbara and Wagner, Christina and Divac Rankov, Aleksandra and Reuter, Sebastian and Bartel, Sabine and Hylkema, Machteld N. and Krueger, Arne and Svanes, Cecilie and Krauss-Etschmann, Susanne",
year = "2018",
abstract = "Background: The prevalence of asthma and chronic obstructive pulmonary disease (COPD) has risen markedly over the last decades and is reaching epidemic proportions. However, underlying molecular mechanisms are not fully understood, hampering the urgently needed development of approaches to prevent these diseases. It is well established from epidemiological studies that prenatal exposure to cigarette smoke is one of the main risk factors for aberrant lung function development or reduced fetal growth, but also for the development of asthma and possibly COPD later in life. Of note, recent evidence suggests that the disease risk can be transferred across generations, that is, from grandparents to their grandchildren. While initial studies in mouse models on in utero smoke exposure have provided important mechanistic insights, there are still knowledge gaps that need to be filled. Objective: Thus, in this review, we summarize current knowledge on this topic derived from mouse models, while also introducing two other relevant animal models: the fruit fly Drosophila melanogaster and the zebrafish Danio rerio. Methods: This review is based on an intensive review of PubMed-listed transgenerational animal studies from 1902 to 2018 and focuses in detail on selected literature due to space limitations. Results: This review gives a comprehensive overview of mechanistic insights obtained in studies with the three species, while highlighting the remaining knowledge gaps. We will further discuss potential (dis)advantages of all three animal models. Conclusion/Clinical Relevance: Many studies have already addressed transgenerational inheritance of disease risk in mouse, zebrafish or fly models. We here propose a novel strategy for how these three model organisms can be synergistically combined to achieve a more detailed understanding of in utero cigarette smoke-induced transgenerational inheritance of disease risk.",
publisher = "Wiley, Hoboken",
journal = "Clinical and Experimental Allergy",
title = "In utero exposure to cigarette smoke and effects across generations: A conference of animals on asthma",
pages = "1390-1378",
number = "11",
volume = "48",
doi = "10.1111/cea.13283"
}

Hammer, B., Wagner, C., Divac Rankov, A., Reuter, S., Bartel, S., Hylkema, M. N., Krueger, A., Svanes, C.,& Krauss-Etschmann, S.. (2018). In utero exposure to cigarette smoke and effects across generations: A conference of animals on asthma. in Clinical and Experimental Allergy
Wiley, Hoboken., 48(11), 1378-1390.
https://doi.org/10.1111/cea.13283

Hammer B, Wagner C, Divac Rankov A, Reuter S, Bartel S, Hylkema MN, Krueger A, Svanes C, Krauss-Etschmann S. In utero exposure to cigarette smoke and effects across generations: A conference of animals on asthma. in Clinical and Experimental Allergy. 2018;48(11):1378-1390.
doi:10.1111/cea.13283 .

Hammer, Barbara, Wagner, Christina, Divac Rankov, Aleksandra, Reuter, Sebastian, Bartel, Sabine, Hylkema, Machteld N., Krueger, Arne, Svanes, Cecilie, Krauss-Etschmann, Susanne, "In utero exposure to cigarette smoke and effects across generations: A conference of animals on asthma" in Clinical and Experimental Allergy, 48, no. 11 (2018):1378-1390,
https://doi.org/10.1111/cea.13283 . .