TY  - JOUR
AU  - Svircev, Milos
AU  - Popsavin, Mirjana
AU  - Pavić, Aleksandar
AU  - Vasiljević, Branka
AU  - Rodić, Marko V.
AU  - Đokić, Sanja
AU  - Kesić, Jelena
AU  - Sreco Zelenović, Bojana
AU  - Popsavin, Velimir
AU  - Kojić, Vesna
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1582
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Bioorganic Chemistry
T1  - Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity (vol 121, 105691, 2022)
VL  - 127
DO  - 10.1016/j.bioorg.2022.105984
ER  - 

@article{
author = "Svircev, Milos and Popsavin, Mirjana and Pavić, Aleksandar and Vasiljević, Branka and Rodić, Marko V. and Đokić, Sanja and Kesić, Jelena and Sreco Zelenović, Bojana and Popsavin, Velimir and Kojić, Vesna",
year = "2022",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Bioorganic Chemistry",
title = "Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity (vol 121, 105691, 2022)",
volume = "127",
doi = "10.1016/j.bioorg.2022.105984"
}

Svircev, M., Popsavin, M., Pavić, A., Vasiljević, B., Rodić, M. V., Đokić, S., Kesić, J., Sreco Zelenović, B., Popsavin, V.,& Kojić, V.. (2022). Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity (vol 121, 105691, 2022). in Bioorganic Chemistry
Academic Press Inc Elsevier Science, San Diego., 127.
https://doi.org/10.1016/j.bioorg.2022.105984

Svircev M, Popsavin M, Pavić A, Vasiljević B, Rodić MV, Đokić S, Kesić J, Sreco Zelenović B, Popsavin V, Kojić V. Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity (vol 121, 105691, 2022). in Bioorganic Chemistry. 2022;127.
doi:10.1016/j.bioorg.2022.105984 .

Svircev, Milos, Popsavin, Mirjana, Pavić, Aleksandar, Vasiljević, Branka, Rodić, Marko V., Đokić, Sanja, Kesić, Jelena, Sreco Zelenović, Bojana, Popsavin, Velimir, Kojić, Vesna, "Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity (vol 121, 105691, 2022)" in Bioorganic Chemistry, 127 (2022),
https://doi.org/10.1016/j.bioorg.2022.105984 . .

TY  - JOUR
AU  - Svircev, Milos
AU  - Popsavin, Mirjana
AU  - Pavić, Aleksandar
AU  - Vasiljević, Branka
AU  - Rodić, Marko V.
AU  - Đokić, Sanja
AU  - Kesić, Jelena
AU  - Sreco Zelenović, Bojana
AU  - Popsavin, Velimir
AU  - Kojić, Vesna
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1561
AB  - The synthesis of several new goniofufurone bioisosteres was achieved in which the phenyl residue was replaced by a thiazole ring. The key steps of the synthesis included the initial condensation of cyanohydrin benzoates with cysteine ethyl ester hydrochloride, followed by the subsequent reaction of resulting C-4 ' epimeric thiazolines with DBU, to introduce 5-deoxy functionality and to elaborate the thiazole ring in one step. Synthesized compounds showed potent growth inhibitory effects against selected human tumour cell lines, especially bioisostere 4, which in the culture of MCF-7 cells displayed the highest activity (IC50 = 0.19 nM) of all compounds under evaluation. This molecule exhibited 64474-fold higher antiproliferative activity than lead 2 and was1053-fold more active than the commercial antitumour agent doxorubicin in the culture of MCF-7 cells. The structural features of the tested compounds responsible for their antiproliferative activity have been identified by preliminary SAR analysis. The toxicity of the most active compound 4 was assessed by an in vivo experiment in a zebrafish model (Danio rerio), whereupon it was found non-toxic at any of the tested concentrations up to 125 mu M.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Bioorganic Chemistry
T1  - Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity
VL  - 121
DO  - 10.1016/j.bioorg.2022.105691
ER  - 

@article{
author = "Svircev, Milos and Popsavin, Mirjana and Pavić, Aleksandar and Vasiljević, Branka and Rodić, Marko V. and Đokić, Sanja and Kesić, Jelena and Sreco Zelenović, Bojana and Popsavin, Velimir and Kojić, Vesna",
year = "2022",
abstract = "The synthesis of several new goniofufurone bioisosteres was achieved in which the phenyl residue was replaced by a thiazole ring. The key steps of the synthesis included the initial condensation of cyanohydrin benzoates with cysteine ethyl ester hydrochloride, followed by the subsequent reaction of resulting C-4 ' epimeric thiazolines with DBU, to introduce 5-deoxy functionality and to elaborate the thiazole ring in one step. Synthesized compounds showed potent growth inhibitory effects against selected human tumour cell lines, especially bioisostere 4, which in the culture of MCF-7 cells displayed the highest activity (IC50 = 0.19 nM) of all compounds under evaluation. This molecule exhibited 64474-fold higher antiproliferative activity than lead 2 and was1053-fold more active than the commercial antitumour agent doxorubicin in the culture of MCF-7 cells. The structural features of the tested compounds responsible for their antiproliferative activity have been identified by preliminary SAR analysis. The toxicity of the most active compound 4 was assessed by an in vivo experiment in a zebrafish model (Danio rerio), whereupon it was found non-toxic at any of the tested concentrations up to 125 mu M.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Bioorganic Chemistry",
title = "Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity",
volume = "121",
doi = "10.1016/j.bioorg.2022.105691"
}

Svircev, M., Popsavin, M., Pavić, A., Vasiljević, B., Rodić, M. V., Đokić, S., Kesić, J., Sreco Zelenović, B., Popsavin, V.,& Kojić, V.. (2022). Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity. in Bioorganic Chemistry
Academic Press Inc Elsevier Science, San Diego., 121.
https://doi.org/10.1016/j.bioorg.2022.105691

Svircev M, Popsavin M, Pavić A, Vasiljević B, Rodić MV, Đokić S, Kesić J, Sreco Zelenović B, Popsavin V, Kojić V. Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity. in Bioorganic Chemistry. 2022;121.
doi:10.1016/j.bioorg.2022.105691 .

Svircev, Milos, Popsavin, Mirjana, Pavić, Aleksandar, Vasiljević, Branka, Rodić, Marko V., Đokić, Sanja, Kesić, Jelena, Sreco Zelenović, Bojana, Popsavin, Velimir, Kojić, Vesna, "Design, synthesis, and biological evaluation of thiazole bioisosteres of goniofufurone through in vitro antiproliferative activity and in vivo toxicity" in Bioorganic Chemistry, 121 (2022),
https://doi.org/10.1016/j.bioorg.2022.105691 . .

TY  - JOUR
AU  - Mojicević, Marija
AU  - D'Agostino, Paul M.
AU  - Pavić, Aleksandar
AU  - Vojnović, Sandra
AU  - Senthamaraikannan, Ramsankar
AU  - Vasiljević, Branka
AU  - Gulder, Tobias A. M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1384
AB  - Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.
PB  - Wiley, Hoboken
T2  - Microbiologyopen
T1  - Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties
IS  - 3
VL  - 9
DO  - 10.1002/mbo3.986
ER  - 

@article{
author = "Mojicević, Marija and D'Agostino, Paul M. and Pavić, Aleksandar and Vojnović, Sandra and Senthamaraikannan, Ramsankar and Vasiljević, Branka and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.",
publisher = "Wiley, Hoboken",
journal = "Microbiologyopen",
title = "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties",
number = "3",
volume = "9",
doi = "10.1002/mbo3.986"
}

Mojicević, M., D'Agostino, P. M., Pavić, A., Vojnović, S., Senthamaraikannan, R., Vasiljević, B., Gulder, T. A. M.,& Nikodinović-Runić, J.. (2020). Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen
Wiley, Hoboken., 9(3).
https://doi.org/10.1002/mbo3.986

Mojicević M, D'Agostino PM, Pavić A, Vojnović S, Senthamaraikannan R, Vasiljević B, Gulder TAM, Nikodinović-Runić J. Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen. 2020;9(3).
doi:10.1002/mbo3.986 .

Mojicević, Marija, D'Agostino, Paul M., Pavić, Aleksandar, Vojnović, Sandra, Senthamaraikannan, Ramsankar, Vasiljević, Branka, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties" in Microbiologyopen, 9, no. 3 (2020),
https://doi.org/10.1002/mbo3.986 . .

TY  - CHAP
AU  - Šenerović, Lidija
AU  - Opsenica, Dejan
AU  - Morić, Ivana
AU  - Aleksić, Ivana
AU  - Spasić, M.
AU  - Vasiljević, Branka
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1348
AB  - Infective diseases have become health threat of a global proportion due to appearance and spread of microorganisms resistant to majority of therapeutics currently used for their treatment. Therefore, there is a constant need for development of new antimicrobial agents, as well as novel therapeutic strategies. Quinolines and quinolones, isolated from plants, animals, and microorganisms, have demonstrated numerous biological activities such as antimicrobial, insecticidal, anti-inflammatory, antiplatelet, and antitumor. For more than two centuries quinoline/quinolone moiety has been used as a scaffold for drug development and even today it represents an inexhaustible inspiration for design and development of novel semi-synthetic or synthetic agents exhibiting broad spectrum of bioactivities. The structural diversity of synthetized compounds provides high and selective activity attained through different mechanisms of action, as well as low toxicity on human cells. This review describes quinoline and quinolone derivatives with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic activities with the focus on the last 10 years literature.
PB  - Springer
T2  - Biophysics of Infection
T1  - Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents
EP  - 69
SP  - 37
VL  - 1282
DO  - 10.1007/5584_2019_428
ER  - 

@inbook{
author = "Šenerović, Lidija and Opsenica, Dejan and Morić, Ivana and Aleksić, Ivana and Spasić, M. and Vasiljević, Branka",
year = "2020",
abstract = "Infective diseases have become health threat of a global proportion due to appearance and spread of microorganisms resistant to majority of therapeutics currently used for their treatment. Therefore, there is a constant need for development of new antimicrobial agents, as well as novel therapeutic strategies. Quinolines and quinolones, isolated from plants, animals, and microorganisms, have demonstrated numerous biological activities such as antimicrobial, insecticidal, anti-inflammatory, antiplatelet, and antitumor. For more than two centuries quinoline/quinolone moiety has been used as a scaffold for drug development and even today it represents an inexhaustible inspiration for design and development of novel semi-synthetic or synthetic agents exhibiting broad spectrum of bioactivities. The structural diversity of synthetized compounds provides high and selective activity attained through different mechanisms of action, as well as low toxicity on human cells. This review describes quinoline and quinolone derivatives with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic activities with the focus on the last 10 years literature.",
publisher = "Springer",
journal = "Biophysics of Infection",
booktitle = "Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents",
pages = "69-37",
volume = "1282",
doi = "10.1007/5584_2019_428"
}

Šenerović, L., Opsenica, D., Morić, I., Aleksić, I., Spasić, M.,& Vasiljević, B.. (2020). Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents. in Biophysics of Infection
Springer., 1282, 37-69.
https://doi.org/10.1007/5584_2019_428

Šenerović L, Opsenica D, Morić I, Aleksić I, Spasić M, Vasiljević B. Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents. in Biophysics of Infection. 2020;1282:37-69.
doi:10.1007/5584_2019_428 .

Šenerović, Lidija, Opsenica, Dejan, Morić, Ivana, Aleksić, Ivana, Spasić, M., Vasiljević, Branka, "Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents" in Biophysics of Infection, 1282 (2020):37-69,
https://doi.org/10.1007/5584_2019_428 . .

TY  - JOUR
AU  - Selaković, Zivota
AU  - Tran, Julie P.
AU  - Kota, Krishna P.
AU  - Lazić, Marija
AU  - Retterer, Cary
AU  - Besh, Robert
AU  - Panchal, Rekha G.
AU  - Soloveva, Veronica
AU  - Sean, Vantongreen A.
AU  - Jay, Wells B.
AU  - Pavić, Aleksandar
AU  - Verbić, Tatjana
AU  - Vasiljević, Branka
AU  - Kuehl, Kathleen
AU  - Duplantier, Allen J.
AU  - Bavari, Sina
AU  - Mudhasani, Rajini
AU  - Solaja, Bogdan A.
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1293
AB  - Ebola virus (EBOV) causes a deadly hemorrhagic fever in humans and non-human primates. There is currently no FDA-approved vaccine or medication to counter this disease. Here, we report on the design, synthesis and anti-viral activities of two classes of compounds which show high potency against EBOV in both in vitro cell culture assays and in vivo mouse models Ebola viral disease. These compounds incorporate the structural features of cationic amphiphilic drugs (CAD), i.e they possess both a hydrophobic domain and a hydrophilic domain consisting of an ionizable amine functional group. These structural features enable easily diffusion into cells but once inside an acidic compartment their amine groups became protonated, ionized and remain trapped inside the acidic compartments such as late endosomes and lysosomes. These compounds, by virtue of their lysomotrophic functions, blocked EBOV entry. However, unlike other drugs containing a CAD moiety including chloroquine and amodiaquine, compounds reported in this study display faster kinetics of accumulation in the lysosomes, robust expansion of late endosome/lysosomes, relatively more potent suppression of lysosome fusion with other vesicular compartments and inhibition of cathepsins activities, all of which play a vital role in anti-EBOV activity. Furthermore, the diazachrysene 2 (ZSML08) that showed most potent activity against EBOV in in vitro cell culture assays also showed significant survival benefit with 100% protection in mouse models of Ebola virus disease, at a low dose of 10 mg/kg/day. Lastly, toxicity studies in vivo using zebrafish models suggest no developmental defects or toxicity associated with these compounds. Overall, these studies describe two new pharmacophores that by virtue of being potent lysosomotrophs, display potent anti-EBOV activities both in vitro and in vivo animal models of EBOV disease.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Second generation of diazachrysenes: Protection of Ebola virus infected mice and mechanism of action
EP  - 50
SP  - 32
VL  - 162
DO  - 10.1016/j.ejmech.2018.10.061
ER  - 

@article{
author = "Selaković, Zivota and Tran, Julie P. and Kota, Krishna P. and Lazić, Marija and Retterer, Cary and Besh, Robert and Panchal, Rekha G. and Soloveva, Veronica and Sean, Vantongreen A. and Jay, Wells B. and Pavić, Aleksandar and Verbić, Tatjana and Vasiljević, Branka and Kuehl, Kathleen and Duplantier, Allen J. and Bavari, Sina and Mudhasani, Rajini and Solaja, Bogdan A.",
year = "2019",
abstract = "Ebola virus (EBOV) causes a deadly hemorrhagic fever in humans and non-human primates. There is currently no FDA-approved vaccine or medication to counter this disease. Here, we report on the design, synthesis and anti-viral activities of two classes of compounds which show high potency against EBOV in both in vitro cell culture assays and in vivo mouse models Ebola viral disease. These compounds incorporate the structural features of cationic amphiphilic drugs (CAD), i.e they possess both a hydrophobic domain and a hydrophilic domain consisting of an ionizable amine functional group. These structural features enable easily diffusion into cells but once inside an acidic compartment their amine groups became protonated, ionized and remain trapped inside the acidic compartments such as late endosomes and lysosomes. These compounds, by virtue of their lysomotrophic functions, blocked EBOV entry. However, unlike other drugs containing a CAD moiety including chloroquine and amodiaquine, compounds reported in this study display faster kinetics of accumulation in the lysosomes, robust expansion of late endosome/lysosomes, relatively more potent suppression of lysosome fusion with other vesicular compartments and inhibition of cathepsins activities, all of which play a vital role in anti-EBOV activity. Furthermore, the diazachrysene 2 (ZSML08) that showed most potent activity against EBOV in in vitro cell culture assays also showed significant survival benefit with 100% protection in mouse models of Ebola virus disease, at a low dose of 10 mg/kg/day. Lastly, toxicity studies in vivo using zebrafish models suggest no developmental defects or toxicity associated with these compounds. Overall, these studies describe two new pharmacophores that by virtue of being potent lysosomotrophs, display potent anti-EBOV activities both in vitro and in vivo animal models of EBOV disease.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Second generation of diazachrysenes: Protection of Ebola virus infected mice and mechanism of action",
pages = "50-32",
volume = "162",
doi = "10.1016/j.ejmech.2018.10.061"
}

Selaković, Z., Tran, J. P., Kota, K. P., Lazić, M., Retterer, C., Besh, R., Panchal, R. G., Soloveva, V., Sean, V. A., Jay, W. B., Pavić, A., Verbić, T., Vasiljević, B., Kuehl, K., Duplantier, A. J., Bavari, S., Mudhasani, R.,& Solaja, B. A.. (2019). Second generation of diazachrysenes: Protection of Ebola virus infected mice and mechanism of action. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 162, 32-50.
https://doi.org/10.1016/j.ejmech.2018.10.061

Selaković Z, Tran JP, Kota KP, Lazić M, Retterer C, Besh R, Panchal RG, Soloveva V, Sean VA, Jay WB, Pavić A, Verbić T, Vasiljević B, Kuehl K, Duplantier AJ, Bavari S, Mudhasani R, Solaja BA. Second generation of diazachrysenes: Protection of Ebola virus infected mice and mechanism of action. in European Journal of Medicinal Chemistry. 2019;162:32-50.
doi:10.1016/j.ejmech.2018.10.061 .

Selaković, Zivota, Tran, Julie P., Kota, Krishna P., Lazić, Marija, Retterer, Cary, Besh, Robert, Panchal, Rekha G., Soloveva, Veronica, Sean, Vantongreen A., Jay, Wells B., Pavić, Aleksandar, Verbić, Tatjana, Vasiljević, Branka, Kuehl, Kathleen, Duplantier, Allen J., Bavari, Sina, Mudhasani, Rajini, Solaja, Bogdan A., "Second generation of diazachrysenes: Protection of Ebola virus infected mice and mechanism of action" in European Journal of Medicinal Chemistry, 162 (2019):32-50,
https://doi.org/10.1016/j.ejmech.2018.10.061 . .

TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Mitić-Culafić, Dragana
AU  - Nebojša, Jasnic
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Vasiljević, Branka
AU  - Knežević-Vukčević, Jelena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1240
AB  - The objective of this study was to evaluate potential of two chemically characterized edible wild onion species, Allium flavum and Allium carinatum, to reduce side effects of cytostatic doxorubicin (Dox). Since Dox application is mainly limited due to its high cardiotoxicity, while there are no approved cardioprotective agents for the prevention of Dox adverse effects, new co-treatments are urgently needed. Here, we showed that methanol extracts expressed high antioxidant activity and synergistically increased Dox anticancer activity against human hepatoma (HepG2) and lung carcinoma (A549) cells, while protected normal human fibroblasts (MRC-5) from Dox cytotoxicity. Analysis of the antioxidative enzymes level (catalase and superoxide dismutases) showed that the catalase level was differently altered in cancer cells compared to normal cells upon applied treatments. In vivo toxicity evaluation in the zebrafish model revealed significantly lower toxicity of extracts compared to Dox, and no teratogenic effects at applied doses. We found that extracts successfully rescued the Dox-treated embryos of life-threating cardiomyopathy, while at the same time reduced developmental toxicity and neutropenia. Further analysis demonstrated that extracts had higher anti-angiogenic activity than sunitinib or auranofin, clinically used anti-angiogenic drugs. In addition, angiogenesis was markedly more suppressed in Dox-extract cotreatments than upon single treatments.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Biomedicine & Pharmacotherapy
T1  - Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin
EP  - 2491
SP  - 2482
VL  - 109
DO  - 10.1016/j.biopha.2018.11.106
ER  - 

@article{
author = "Pavić, Aleksandar and Mitić-Culafić, Dragana and Nebojša, Jasnic and Nikolić, Biljana and Simin, Nataša and Vasiljević, Branka and Knežević-Vukčević, Jelena",
year = "2019",
abstract = "The objective of this study was to evaluate potential of two chemically characterized edible wild onion species, Allium flavum and Allium carinatum, to reduce side effects of cytostatic doxorubicin (Dox). Since Dox application is mainly limited due to its high cardiotoxicity, while there are no approved cardioprotective agents for the prevention of Dox adverse effects, new co-treatments are urgently needed. Here, we showed that methanol extracts expressed high antioxidant activity and synergistically increased Dox anticancer activity against human hepatoma (HepG2) and lung carcinoma (A549) cells, while protected normal human fibroblasts (MRC-5) from Dox cytotoxicity. Analysis of the antioxidative enzymes level (catalase and superoxide dismutases) showed that the catalase level was differently altered in cancer cells compared to normal cells upon applied treatments. In vivo toxicity evaluation in the zebrafish model revealed significantly lower toxicity of extracts compared to Dox, and no teratogenic effects at applied doses. We found that extracts successfully rescued the Dox-treated embryos of life-threating cardiomyopathy, while at the same time reduced developmental toxicity and neutropenia. Further analysis demonstrated that extracts had higher anti-angiogenic activity than sunitinib or auranofin, clinically used anti-angiogenic drugs. In addition, angiogenesis was markedly more suppressed in Dox-extract cotreatments than upon single treatments.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Biomedicine & Pharmacotherapy",
title = "Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin",
pages = "2491-2482",
volume = "109",
doi = "10.1016/j.biopha.2018.11.106"
}

Pavić, A., Mitić-Culafić, D., Nebojša, J., Nikolić, B., Simin, N., Vasiljević, B.,& Knežević-Vukčević, J.. (2019). Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin. in Biomedicine & Pharmacotherapy
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 109, 2482-2491.
https://doi.org/10.1016/j.biopha.2018.11.106

Pavić A, Mitić-Culafić D, Nebojša J, Nikolić B, Simin N, Vasiljević B, Knežević-Vukčević J. Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin. in Biomedicine & Pharmacotherapy. 2019;109:2482-2491.
doi:10.1016/j.biopha.2018.11.106 .

Pavić, Aleksandar, Mitić-Culafić, Dragana, Nebojša, Jasnic, Nikolić, Biljana, Simin, Nataša, Vasiljević, Branka, Knežević-Vukčević, Jelena, "Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin" in Biomedicine & Pharmacotherapy, 109 (2019):2482-2491,
https://doi.org/10.1016/j.biopha.2018.11.106 . .

TY  - JOUR
AU  - Mojicević, Marija
AU  - D'Agostino, Paul M.
AU  - Nikodinović-Runić, Jasmina
AU  - Vasiljević, Branka
AU  - Gulder, Tobias A. M.
AU  - Vojnović, Sandra
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1212
AB  - The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 mu g/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds.
PB  - Springer, New York
T2  - International Microbiology
T1  - Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)
EP  - 353
IS  - 3
SP  - 343
VL  - 22
DO  - 10.1007/s10123-019-00054-8
ER  - 

@article{
author = "Mojicević, Marija and D'Agostino, Paul M. and Nikodinović-Runić, Jasmina and Vasiljević, Branka and Gulder, Tobias A. M. and Vojnović, Sandra",
year = "2019",
abstract = "The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 mu g/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds.",
publisher = "Springer, New York",
journal = "International Microbiology",
title = "Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)",
pages = "353-343",
number = "3",
volume = "22",
doi = "10.1007/s10123-019-00054-8"
}

Mojicević, M., D'Agostino, P. M., Nikodinović-Runić, J., Vasiljević, B., Gulder, T. A. M.,& Vojnović, S.. (2019). Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle). in International Microbiology
Springer, New York., 22(3), 343-353.
https://doi.org/10.1007/s10123-019-00054-8

Mojicević M, D'Agostino PM, Nikodinović-Runić J, Vasiljević B, Gulder TAM, Vojnović S. Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle). in International Microbiology. 2019;22(3):343-353.
doi:10.1007/s10123-019-00054-8 .

Mojicević, Marija, D'Agostino, Paul M., Nikodinović-Runić, Jasmina, Vasiljević, Branka, Gulder, Tobias A. M., Vojnović, Sandra, "Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)" in International Microbiology, 22, no. 3 (2019):343-353,
https://doi.org/10.1007/s10123-019-00054-8 . .

TY  - JOUR
AU  - Spasić, Jelena
AU  - Mandić, Mina
AU  - Radivojević, Jelena
AU  - Jeremić, Sanja
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Đokić, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1134
AB  - Biocatalytic potential of Streptomyces strains isolated from the rhizosphere of plants and from mycorrhizosphere of fungi has been investigated. A total of 118 Streptomyces isolates were selected and functionally screened for 10 different biotechnologically important enzymatic activities: hydrolase (cellulase, cutinase, gelatinase, lipase, protease, polyhydroxyalkanoate (PHA) depolymerase), phenol oxidase and peroxidase (laccase, tyrosinase, and lignin peroxidase), and aminotransferase. Out of 118 tested Streptomyces spp., 90% showed at least one enzymatic activity. The most abundant were enzymes involved in the biomass degradation, as the production of cutinase, cellulase, and lignin peroxidase were detected in 31%, 40%, and 48% of the isolates, respectively. The improved specific activities of lipase (isolates BV315 and BV100) and tyrosinase (isolates BV87 and BV88) were shown in comparison with the industrially relevant activities of Pseudomonas strains. Plant rhizosphere soils were more prolific source of Streptomyces strains with biocatalytic potential in comparison with mycorrhizosphere soils. Overall, 284 enzyme activities among 118 Streptomyces isolates have been detected. This is the first comprehensive screening of Streptomyces isolates from rhizosphere and mycorrhizosphere soils for novel biocatalysts, showing that specific environmental habitats, such as rhizosphere soils, are treasure troves of Streptomyces with biocatalytic potential.
PB  - Wiley, Hoboken
T2  - Biotechnology and Applied Biochemistry
T1  - Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi
EP  - 833
IS  - 6
SP  - 822
VL  - 65
DO  - 10.1002/bab.1664
ER  - 

@article{
author = "Spasić, Jelena and Mandić, Mina and Radivojević, Jelena and Jeremić, Sanja and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Đokić, Lidija",
year = "2018",
abstract = "Biocatalytic potential of Streptomyces strains isolated from the rhizosphere of plants and from mycorrhizosphere of fungi has been investigated. A total of 118 Streptomyces isolates were selected and functionally screened for 10 different biotechnologically important enzymatic activities: hydrolase (cellulase, cutinase, gelatinase, lipase, protease, polyhydroxyalkanoate (PHA) depolymerase), phenol oxidase and peroxidase (laccase, tyrosinase, and lignin peroxidase), and aminotransferase. Out of 118 tested Streptomyces spp., 90% showed at least one enzymatic activity. The most abundant were enzymes involved in the biomass degradation, as the production of cutinase, cellulase, and lignin peroxidase were detected in 31%, 40%, and 48% of the isolates, respectively. The improved specific activities of lipase (isolates BV315 and BV100) and tyrosinase (isolates BV87 and BV88) were shown in comparison with the industrially relevant activities of Pseudomonas strains. Plant rhizosphere soils were more prolific source of Streptomyces strains with biocatalytic potential in comparison with mycorrhizosphere soils. Overall, 284 enzyme activities among 118 Streptomyces isolates have been detected. This is the first comprehensive screening of Streptomyces isolates from rhizosphere and mycorrhizosphere soils for novel biocatalysts, showing that specific environmental habitats, such as rhizosphere soils, are treasure troves of Streptomyces with biocatalytic potential.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Applied Biochemistry",
title = "Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi",
pages = "833-822",
number = "6",
volume = "65",
doi = "10.1002/bab.1664"
}

Spasić, J., Mandić, M., Radivojević, J., Jeremić, S., Vasiljević, B., Nikodinović-Runić, J.,& Đokić, L.. (2018). Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi. in Biotechnology and Applied Biochemistry
Wiley, Hoboken., 65(6), 822-833.
https://doi.org/10.1002/bab.1664

Spasić J, Mandić M, Radivojević J, Jeremić S, Vasiljević B, Nikodinović-Runić J, Đokić L. Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi. in Biotechnology and Applied Biochemistry. 2018;65(6):822-833.
doi:10.1002/bab.1664 .

Spasić, Jelena, Mandić, Mina, Radivojević, Jelena, Jeremić, Sanja, Vasiljević, Branka, Nikodinović-Runić, Jasmina, Đokić, Lidija, "Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi" in Biotechnology and Applied Biochemistry, 65, no. 6 (2018):822-833,
https://doi.org/10.1002/bab.1664 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Comić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojkovic-Opsenica, Dusanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1762
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
EP  - 158
SP  - 148
VL  - 222
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Comić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojkovic-Opsenica, Dusanka and Šenerović, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
pages = "158-148",
volume = "222",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D.,& Šenerović, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojević P, Pavić A, Radojević I, Comić LR, Vasiljević B, Opsenica D, Milojkovic-Opsenica D, Šenerović L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Comić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojkovic-Opsenica, Dusanka, Šenerović, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .

TY  - DATA
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Comić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojkovic-Opsenica, Dusanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1768
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005
VL  - 222
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1768
ER  - 

@misc{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Comić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojkovic-Opsenica, Dusanka and Šenerović, Lidija",
year = "2018",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005",
volume = "222",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1768"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D.,& Šenerović, L.. (2018). Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222.
https://hdl.handle.net/21.15107/rcub_imagine_1768

Aleksić I, Ristivojević P, Pavić A, Radojević I, Comić LR, Vasiljević B, Opsenica D, Milojkovic-Opsenica D, Šenerović L. Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005. in Journal of Ethnopharmacology. 2018;222.
https://hdl.handle.net/21.15107/rcub_imagine_1768 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Comić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojkovic-Opsenica, Dusanka, Šenerović, Lidija, "Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005" in Journal of Ethnopharmacology, 222 (2018),
https://hdl.handle.net/21.15107/rcub_imagine_1768 .

TY  - JOUR
AU  - Milivojević, Dušan
AU  - Sumonja, Neven
AU  - Medić, Strahinja
AU  - Pavić, Aleksandar
AU  - Morić, Ivana
AU  - Vasiljević, Branka
AU  - Šenerović, Lidija
AU  - Nikodinović-Runić, Jasmina
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1117
AB  - Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability.
PB  - Oxford Univ Press, Oxford
T2  - Pathogens and Disease
T1  - Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans
IS  - 4
VL  - 76
DO  - 10.1093/femspd/fty041
ER  - 

@article{
author = "Milivojević, Dušan and Sumonja, Neven and Medić, Strahinja and Pavić, Aleksandar and Morić, Ivana and Vasiljević, Branka and Šenerović, Lidija and Nikodinović-Runić, Jasmina",
year = "2018",
abstract = "Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability.",
publisher = "Oxford Univ Press, Oxford",
journal = "Pathogens and Disease",
title = "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans",
number = "4",
volume = "76",
doi = "10.1093/femspd/fty041"
}

Milivojević, D., Sumonja, N., Medić, S., Pavić, A., Morić, I., Vasiljević, B., Šenerović, L.,& Nikodinović-Runić, J.. (2018). Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease
Oxford Univ Press, Oxford., 76(4).
https://doi.org/10.1093/femspd/fty041

Milivojević D, Sumonja N, Medić S, Pavić A, Morić I, Vasiljević B, Šenerović L, Nikodinović-Runić J. Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease. 2018;76(4).
doi:10.1093/femspd/fty041 .

Milivojević, Dušan, Sumonja, Neven, Medić, Strahinja, Pavić, Aleksandar, Morić, Ivana, Vasiljević, Branka, Šenerović, Lidija, Nikodinović-Runić, Jasmina, "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans" in Pathogens and Disease, 76, no. 4 (2018),
https://doi.org/10.1093/femspd/fty041 . .

TY  - JOUR
AU  - Schneider, Olha
AU  - Ilić-Tomić, Tatjana
AU  - Rueckert, Christian
AU  - Kalinowski, Joern
AU  - Gencić, Marija S.
AU  - Zivković, Milena Z.
AU  - Stanković, Nada
AU  - Radulović, Niko S.
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Zotchev, Sergey B.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1107
AB  - Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved. Analysis of the Streptomyces sp. NP10 draft genome revealed that it is closely related to several strains of Streptomyces griseus. Comparative analyses of secondary metabolite biosynthetic gene clusters, as well as those presumably involved in FA biosynthesis, allowed identification of an unusual cluster C12-2, which could be identified in only one other S. griseus-related streptomycete. To prove the involvement of identified cluster in FFA biosynthesis, one of its three ketosynthase genes was insertionally inactivated to generate mutant strain mNP10. Accumulation of FFAs in mNP10 was almost completely abolished, reaching less than 0.01% compared to the wild-type strain. Cloning and transfer of the C12-2 cluster to the mNP10 mutant partially restored FFA production, albeit to a low level. The discovery of this rare FFA biosynthesis cluster opens possibilities for detailed characterization of the roles of individual genes and their products in the biosynthesis of FFAs in NP10.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10
VL  - 9
DO  - 10.3389/fmicb.2018.01302
ER  - 

@article{
author = "Schneider, Olha and Ilić-Tomić, Tatjana and Rueckert, Christian and Kalinowski, Joern and Gencić, Marija S. and Zivković, Milena Z. and Stanković, Nada and Radulović, Niko S. and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Zotchev, Sergey B.",
year = "2018",
abstract = "Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved. Analysis of the Streptomyces sp. NP10 draft genome revealed that it is closely related to several strains of Streptomyces griseus. Comparative analyses of secondary metabolite biosynthetic gene clusters, as well as those presumably involved in FA biosynthesis, allowed identification of an unusual cluster C12-2, which could be identified in only one other S. griseus-related streptomycete. To prove the involvement of identified cluster in FFA biosynthesis, one of its three ketosynthase genes was insertionally inactivated to generate mutant strain mNP10. Accumulation of FFAs in mNP10 was almost completely abolished, reaching less than 0.01% compared to the wild-type strain. Cloning and transfer of the C12-2 cluster to the mNP10 mutant partially restored FFA production, albeit to a low level. The discovery of this rare FFA biosynthesis cluster opens possibilities for detailed characterization of the roles of individual genes and their products in the biosynthesis of FFAs in NP10.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10",
volume = "9",
doi = "10.3389/fmicb.2018.01302"
}

Schneider, O., Ilić-Tomić, T., Rueckert, C., Kalinowski, J., Gencić, M. S., Zivković, M. Z., Stanković, N., Radulović, N. S., Vasiljević, B., Nikodinović-Runić, J.,& Zotchev, S. B.. (2018). Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.01302

Schneider O, Ilić-Tomić T, Rueckert C, Kalinowski J, Gencić MS, Zivković MZ, Stanković N, Radulović NS, Vasiljević B, Nikodinović-Runić J, Zotchev SB. Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.01302 .

Schneider, Olha, Ilić-Tomić, Tatjana, Rueckert, Christian, Kalinowski, Joern, Gencić, Marija S., Zivković, Milena Z., Stanković, Nada, Radulović, Niko S., Vasiljević, Branka, Nikodinović-Runić, Jasmina, Zotchev, Sergey B., "Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.01302 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Comić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojkovic-Opsenica, Dusanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1111
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
EP  - 158
SP  - 148
VL  - 222
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Comić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojkovic-Opsenica, Dusanka and Šenerović, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
pages = "158-148",
volume = "222",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D.,& Šenerović, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojević P, Pavić A, Radojević I, Comić LR, Vasiljević B, Opsenica D, Milojkovic-Opsenica D, Šenerović L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Comić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojkovic-Opsenica, Dusanka, Šenerović, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Petković, Milos
AU  - Jovanović, Milos
AU  - Milivojević, Dušan
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Šenerović, Lidija
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1033
AB  - A new strain, namely Lysinibacillus sp. BV152.1 was isolated from the rhizosphere of ground ivy (Glechoma hederacea L.) producing metabolites with potent ability to inhibit biofilm formation of an important human pathogens Pseudomonas aeruginosa PAO1, Staphylococcus aureus, and Serratia marcescens. Structural characterization revealed di-rhamnolipids mixture containing rhamnose (Rha)-Rha-C10-C10, Rha-Rha-C8-C10, and Rha-Rha-C10-C12 in the ratio 7: 2: 1 as the active principle. Purified di-rhamnolipids, as well as commercially available di-rhamnolipids (Rha-Rha-C10-C10, 93%) were used as the substrate for the chemical derivatization for the first time, yielding three semisynthetic amide derivatives, benzyl-, piperidine-, and morpholine. A comparative study of the anti-biofilm, antibacterial and cytotoxic properties revealed that di-Rha from Lysinibacillus sp. BV152.1 were more potent in biofilm inhibition, both cell adhesion and biofilm maturation, than commercial di-rhamnolipids inhibiting 50% of P. aeruginosa PAO1 biofilm formation at 50 mu g mL(-1) and 75 mu g mL(-1), respectively. None of the dirhamnolipids exhibited antimicrobial properties at concentrations of up to 500 mu g mL(-1). Amide derivatization improved inhibition of biofilm formation and dispersion activities of di-rhamnolipids from both sources, with morpholine derivative being the most active causing more than 80% biofilm inhibition at concentrations 100 mu g mL(-1). Semisynthetic amide derivatives showed increased antibacterial activity against S. aureus, and also showed higher cytotoxicity. Therefore, described di-rhamnolipids are potent anti-biofilm agents and the described approach can be seen as viable approach in reaching new rhamnolipid based derivatives with tailored biological properties.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Anti-biofilm Properties of Bacterial Di-Rhamnolipids and Their Semi-Synthetic Amide Derivatives
VL  - 8
DO  - 10.3389/fmicb.2017.02454
ER  - 

@article{
author = "Aleksić, Ivana and Petković, Milos and Jovanović, Milos and Milivojević, Dušan and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Šenerović, Lidija",
year = "2017",
abstract = "A new strain, namely Lysinibacillus sp. BV152.1 was isolated from the rhizosphere of ground ivy (Glechoma hederacea L.) producing metabolites with potent ability to inhibit biofilm formation of an important human pathogens Pseudomonas aeruginosa PAO1, Staphylococcus aureus, and Serratia marcescens. Structural characterization revealed di-rhamnolipids mixture containing rhamnose (Rha)-Rha-C10-C10, Rha-Rha-C8-C10, and Rha-Rha-C10-C12 in the ratio 7: 2: 1 as the active principle. Purified di-rhamnolipids, as well as commercially available di-rhamnolipids (Rha-Rha-C10-C10, 93%) were used as the substrate for the chemical derivatization for the first time, yielding three semisynthetic amide derivatives, benzyl-, piperidine-, and morpholine. A comparative study of the anti-biofilm, antibacterial and cytotoxic properties revealed that di-Rha from Lysinibacillus sp. BV152.1 were more potent in biofilm inhibition, both cell adhesion and biofilm maturation, than commercial di-rhamnolipids inhibiting 50% of P. aeruginosa PAO1 biofilm formation at 50 mu g mL(-1) and 75 mu g mL(-1), respectively. None of the dirhamnolipids exhibited antimicrobial properties at concentrations of up to 500 mu g mL(-1). Amide derivatization improved inhibition of biofilm formation and dispersion activities of di-rhamnolipids from both sources, with morpholine derivative being the most active causing more than 80% biofilm inhibition at concentrations 100 mu g mL(-1). Semisynthetic amide derivatives showed increased antibacterial activity against S. aureus, and also showed higher cytotoxicity. Therefore, described di-rhamnolipids are potent anti-biofilm agents and the described approach can be seen as viable approach in reaching new rhamnolipid based derivatives with tailored biological properties.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Anti-biofilm Properties of Bacterial Di-Rhamnolipids and Their Semi-Synthetic Amide Derivatives",
volume = "8",
doi = "10.3389/fmicb.2017.02454"
}

Aleksić, I., Petković, M., Jovanović, M., Milivojević, D., Vasiljević, B., Nikodinović-Runić, J.,& Šenerović, L.. (2017). Anti-biofilm Properties of Bacterial Di-Rhamnolipids and Their Semi-Synthetic Amide Derivatives. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.02454

Aleksić I, Petković M, Jovanović M, Milivojević D, Vasiljević B, Nikodinović-Runić J, Šenerović L. Anti-biofilm Properties of Bacterial Di-Rhamnolipids and Their Semi-Synthetic Amide Derivatives. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.02454 .

Aleksić, Ivana, Petković, Milos, Jovanović, Milos, Milivojević, Dušan, Vasiljević, Branka, Nikodinović-Runić, Jasmina, Šenerović, Lidija, "Anti-biofilm Properties of Bacterial Di-Rhamnolipids and Their Semi-Synthetic Amide Derivatives" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.02454 . .

TY  - JOUR
AU  - Kiskaroly, Ferenc
AU  - Morić, Ivana
AU  - Đokić, Lidija
AU  - Vasiljević, Branka
AU  - Šenerović, Lidija
AU  - Mišić, Dušan
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1012
AB  - The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with S. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Development of pcr-based identification of salmonella enterica serovars
EP  - 290
IS  - 2
SP  - 277
VL  - 67
DO  - 10.1515/acve-2017-0022
ER  - 

@article{
author = "Kiskaroly, Ferenc and Morić, Ivana and Đokić, Lidija and Vasiljević, Branka and Šenerović, Lidija and Mišić, Dušan",
year = "2017",
abstract = "The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with S. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Development of pcr-based identification of salmonella enterica serovars",
pages = "290-277",
number = "2",
volume = "67",
doi = "10.1515/acve-2017-0022"
}

Kiskaroly, F., Morić, I., Đokić, L., Vasiljević, B., Šenerović, L.,& Mišić, D.. (2017). Development of pcr-based identification of salmonella enterica serovars. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 277-290.
https://doi.org/10.1515/acve-2017-0022

Kiskaroly F, Morić I, Đokić L, Vasiljević B, Šenerović L, Mišić D. Development of pcr-based identification of salmonella enterica serovars. in Acta Veterinaria-Beograd. 2017;67(2):277-290.
doi:10.1515/acve-2017-0022 .

Kiskaroly, Ferenc, Morić, Ivana, Đokić, Lidija, Vasiljević, Branka, Šenerović, Lidija, Mišić, Dušan, "Development of pcr-based identification of salmonella enterica serovars" in Acta Veterinaria-Beograd, 67, no. 2 (2017):277-290,
https://doi.org/10.1515/acve-2017-0022 . .

TY  - JOUR
AU  - Stanković, Srdan
AU  - Vasiljević, Branka
AU  - Jeremić, Sanja
AU  - Cvetković, Vladica
AU  - Morić, Ivana
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1084
AB  - Extremely acidic environments are frequently formed in areas impacted by mining activities, and Lake Robule is such an ecosystem. Although an extreme environment, Lake Robule is inhabited by acidophilic microorganisms. We investigated biodiversity of the macroscopic structure known as a microbial mat formed on the lake bottom in shallow waters. Microbial mats are common in acidic environments, but their composition can differ significantly from site to site. Microbial diversity of the mat from Lake Robule was investigated using both cultivation-dependent and metagenomic approaches. The results showed the mat to be mostly inhabited by heterotrophic acidophilic bacteria. When compared to the microbial community of Lake Robule's surface water, the microbial mat proved to be a more complex community. A biogeochemical model of the mat of Lake Robule is proposed on the basis of our results and available published data.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia)
EP  - 54
IS  - 1
SP  - 47
VL  - 41
DO  - 10.5281/zenodo.453792
ER  - 

@article{
author = "Stanković, Srdan and Vasiljević, Branka and Jeremić, Sanja and Cvetković, Vladica and Morić, Ivana",
year = "2017",
abstract = "Extremely acidic environments are frequently formed in areas impacted by mining activities, and Lake Robule is such an ecosystem. Although an extreme environment, Lake Robule is inhabited by acidophilic microorganisms. We investigated biodiversity of the macroscopic structure known as a microbial mat formed on the lake bottom in shallow waters. Microbial mats are common in acidic environments, but their composition can differ significantly from site to site. Microbial diversity of the mat from Lake Robule was investigated using both cultivation-dependent and metagenomic approaches. The results showed the mat to be mostly inhabited by heterotrophic acidophilic bacteria. When compared to the microbial community of Lake Robule's surface water, the microbial mat proved to be a more complex community. A biogeochemical model of the mat of Lake Robule is proposed on the basis of our results and available published data.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia)",
pages = "54-47",
number = "1",
volume = "41",
doi = "10.5281/zenodo.453792"
}

Stanković, S., Vasiljević, B., Jeremić, S., Cvetković, V.,& Morić, I.. (2017). Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia). in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 41(1), 47-54.
https://doi.org/10.5281/zenodo.453792

Stanković S, Vasiljević B, Jeremić S, Cvetković V, Morić I. Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia). in Botanica Serbica. 2017;41(1):47-54.
doi:10.5281/zenodo.453792 .

Stanković, Srdan, Vasiljević, Branka, Jeremić, Sanja, Cvetković, Vladica, Morić, Ivana, "Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia)" in Botanica Serbica, 41, no. 1 (2017):47-54,
https://doi.org/10.5281/zenodo.453792 . .

TY  - JOUR
AU  - Jeremić, Sanja
AU  - Beskoski, Vladimir P.
AU  - Đokić, Lidija
AU  - Vasiljević, Branka
AU  - Vrvić, Miroslav M.
AU  - Avdalović, Jelena
AU  - Cvijović, Gordana Gojgic
AU  - Beskoski, Latinka Slavkovic
AU  - Nikodinović-Runić, Jasmina
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/984
AB  - Iron and sulfur oxidizing chemolithoautotrophic acidophilic bacteria, such as Acidithiobacillus species, hold the dominant role in mine environments characterized by low pH values and high concentrations of reduced sulfur and iron compounds, such as ores, rocks and acid drainage waters from mines. On the other hand, heterotrophic microorganisms, especially their biofilms, from these specific niches are receiving increased attention, but their potential eco-physiological roles have not been fully understood. Biofilms are considered a threat to human health, but biofilms also have beneficial properties as they are deployed in waste recycling and bioremediation systems. We have analyzed interactions of the metal tolerant heterotrophic microorganisms in biofilms with iron oxidizing autotrophic bacteria both from the sulphidic mine environment (copper mine Bor, Serbia). High tolerance to Cu2+, Cd2+ and Cr6+ and the presence of genetic determinants for the respective metal tolerance and biofilm-forming ability was shown for indigenous heterotrophic bacteria that included strains of Staphylococcus and Rhodococcus. Two well characterized bacteria-Pseudomonas aeruginosa PAO1 (known biofilm former) and Cupriavidus metallidurans CH34 (known metal resistant representative) were also included in the study. The interaction and survivability of autotrophic iron oxidizing Acidithiobacillus bacteria and biofilms of heterotrophic bacteria during co-cultivation was revealed. Finally, the effect of heterotrophic biofilms on bioleaching process with indigenous iron oxidizing Acidithiobacillus species was shown not to be inhibitory under in vitro conditions.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Journal of Environmental Management
T1  - Interactions of the metal tolerant heterotrophic microorganisms and iron oxidizing autotrophic bacteria from sulphidic mine environment during bioleaching experiments
EP  - 161
SP  - 151
VL  - 172
DO  - 10.1016/j.jenvman.2016.02.041
ER  - 

@article{
author = "Jeremić, Sanja and Beskoski, Vladimir P. and Đokić, Lidija and Vasiljević, Branka and Vrvić, Miroslav M. and Avdalović, Jelena and Cvijović, Gordana Gojgic and Beskoski, Latinka Slavkovic and Nikodinović-Runić, Jasmina",
year = "2016",
abstract = "Iron and sulfur oxidizing chemolithoautotrophic acidophilic bacteria, such as Acidithiobacillus species, hold the dominant role in mine environments characterized by low pH values and high concentrations of reduced sulfur and iron compounds, such as ores, rocks and acid drainage waters from mines. On the other hand, heterotrophic microorganisms, especially their biofilms, from these specific niches are receiving increased attention, but their potential eco-physiological roles have not been fully understood. Biofilms are considered a threat to human health, but biofilms also have beneficial properties as they are deployed in waste recycling and bioremediation systems. We have analyzed interactions of the metal tolerant heterotrophic microorganisms in biofilms with iron oxidizing autotrophic bacteria both from the sulphidic mine environment (copper mine Bor, Serbia). High tolerance to Cu2+, Cd2+ and Cr6+ and the presence of genetic determinants for the respective metal tolerance and biofilm-forming ability was shown for indigenous heterotrophic bacteria that included strains of Staphylococcus and Rhodococcus. Two well characterized bacteria-Pseudomonas aeruginosa PAO1 (known biofilm former) and Cupriavidus metallidurans CH34 (known metal resistant representative) were also included in the study. The interaction and survivability of autotrophic iron oxidizing Acidithiobacillus bacteria and biofilms of heterotrophic bacteria during co-cultivation was revealed. Finally, the effect of heterotrophic biofilms on bioleaching process with indigenous iron oxidizing Acidithiobacillus species was shown not to be inhibitory under in vitro conditions.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Journal of Environmental Management",
title = "Interactions of the metal tolerant heterotrophic microorganisms and iron oxidizing autotrophic bacteria from sulphidic mine environment during bioleaching experiments",
pages = "161-151",
volume = "172",
doi = "10.1016/j.jenvman.2016.02.041"
}

Jeremić, S., Beskoski, V. P., Đokić, L., Vasiljević, B., Vrvić, M. M., Avdalović, J., Cvijović, G. G., Beskoski, L. S.,& Nikodinović-Runić, J.. (2016). Interactions of the metal tolerant heterotrophic microorganisms and iron oxidizing autotrophic bacteria from sulphidic mine environment during bioleaching experiments. in Journal of Environmental Management
Academic Press Ltd- Elsevier Science Ltd, London., 172, 151-161.
https://doi.org/10.1016/j.jenvman.2016.02.041

Jeremić S, Beskoski VP, Đokić L, Vasiljević B, Vrvić MM, Avdalović J, Cvijović GG, Beskoski LS, Nikodinović-Runić J. Interactions of the metal tolerant heterotrophic microorganisms and iron oxidizing autotrophic bacteria from sulphidic mine environment during bioleaching experiments. in Journal of Environmental Management. 2016;172:151-161.
doi:10.1016/j.jenvman.2016.02.041 .

Jeremić, Sanja, Beskoski, Vladimir P., Đokić, Lidija, Vasiljević, Branka, Vrvić, Miroslav M., Avdalović, Jelena, Cvijović, Gordana Gojgic, Beskoski, Latinka Slavkovic, Nikodinović-Runić, Jasmina, "Interactions of the metal tolerant heterotrophic microorganisms and iron oxidizing autotrophic bacteria from sulphidic mine environment during bioleaching experiments" in Journal of Environmental Management, 172 (2016):151-161,
https://doi.org/10.1016/j.jenvman.2016.02.041 . .

TY  - JOUR
AU  - Pekmezović, Marina
AU  - Aleksić, Ivana
AU  - Barac, Aleksandra
AU  - Arsić-Arsenijević, Valentina
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Šenerović, Lidija
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/953
AB  - Mixed microbial infections caused by Pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. The aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (EOs). The mechanism of antimicrobial activity of EOs was analysed. EOs of pompia and grapefruit inhibited fungal growth with MIC concentrations between 50 and 250 mg L-1, whereas no effect on P. aeruginosa growth was observed. Both citrus EOs inhibited formation of bacterial and fungal monomicrobial biofilms in concentrations of 50 mg L-1 and were efficient in potentiating the activity of clinically used antimicrobials in vitro. The concentration of 10 mg L-1 EOs inhibited mixed biofilm formation composed of P. aeruginosa and Aspergillus fumigatus or Scedosporium apiospermum. Citrus EOs affected quorum sensing in P. aeruginosa and caused fast permeabilisation of Candida albicans membrane. Pompia and grapefruit EOs potently inhibited biofilm formation and could be used for the control of common polymicrobial infections.
PB  - Oxford Univ Press, Oxford
T2  - Pathogens and Disease
T1  - Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species
IS  - 8
VL  - 74
DO  - 10.1093/femspd/ftw102
ER  - 

@article{
author = "Pekmezović, Marina and Aleksić, Ivana and Barac, Aleksandra and Arsić-Arsenijević, Valentina and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Šenerović, Lidija",
year = "2016",
abstract = "Mixed microbial infections caused by Pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. The aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (EOs). The mechanism of antimicrobial activity of EOs was analysed. EOs of pompia and grapefruit inhibited fungal growth with MIC concentrations between 50 and 250 mg L-1, whereas no effect on P. aeruginosa growth was observed. Both citrus EOs inhibited formation of bacterial and fungal monomicrobial biofilms in concentrations of 50 mg L-1 and were efficient in potentiating the activity of clinically used antimicrobials in vitro. The concentration of 10 mg L-1 EOs inhibited mixed biofilm formation composed of P. aeruginosa and Aspergillus fumigatus or Scedosporium apiospermum. Citrus EOs affected quorum sensing in P. aeruginosa and caused fast permeabilisation of Candida albicans membrane. Pompia and grapefruit EOs potently inhibited biofilm formation and could be used for the control of common polymicrobial infections.",
publisher = "Oxford Univ Press, Oxford",
journal = "Pathogens and Disease",
title = "Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species",
number = "8",
volume = "74",
doi = "10.1093/femspd/ftw102"
}

Pekmezović, M., Aleksić, I., Barac, A., Arsić-Arsenijević, V., Vasiljević, B., Nikodinović-Runić, J.,& Šenerović, L.. (2016). Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species. in Pathogens and Disease
Oxford Univ Press, Oxford., 74(8).
https://doi.org/10.1093/femspd/ftw102

Pekmezović M, Aleksić I, Barac A, Arsić-Arsenijević V, Vasiljević B, Nikodinović-Runić J, Šenerović L. Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species. in Pathogens and Disease. 2016;74(8).
doi:10.1093/femspd/ftw102 .

Pekmezović, Marina, Aleksić, Ivana, Barac, Aleksandra, Arsić-Arsenijević, Valentina, Vasiljević, Branka, Nikodinović-Runić, Jasmina, Šenerović, Lidija, "Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species" in Pathogens and Disease, 74, no. 8 (2016),
https://doi.org/10.1093/femspd/ftw102 . .

TY  - JOUR
AU  - Savić, Nada D.
AU  - Milivojević, Dušan
AU  - Glišić, Biljana
AU  - Ilić-Tomić, Tatjana
AU  - Veselinović, Jovana
AU  - Pavić, Aleksandar
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Djuran, Milos I.
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/978
AB  - Five aromatic nitrogen-containing heterocycles, pyridazine (pydz, 1), pyrimidine (pm, 2), pyrazine (pz, 3), quinoxaline (qx, 4) and phenazine (phz, 5) have been used for the synthesis of gold(III) and silver(I) complexes. In contrast to the mononuclear Au1-5 complexes all having square-planar geometry, the corresponding Ag1-5 complexes have been found to be polynuclear and of different geometries. Complexes Au1-5 and Ag1-5, along with K[AuCl4], AgNO3 and N-heterocyclic ligands used for their synthesis, were evaluated by in vitro antimicrobial studies against a panel of microbial strains that lead to many skin and soft tissue, respiratory, wound and nosocomial infections. All tested complexes exhibited excellent to good antibacterial activity with minimal inhibitory (MIC) values in the range of 2.5 to 100 mu g mL(-1) against the investigated strains. The complexes were particularly efficient against pathogenic Pseudomonas aeruginosa (MIC = 2.5-30 mu g mL(-1)) and had a marked ability to disrupt clinically relevant biofilms of strains with high inherent resistance to antibiotics. Moreover, the Au1-4 and Ag1-5 complexes exhibited pronounced ability to competitively intercalate double stranded genomic DNA of P. aeruginosa, which was demonstrated by gel electrophoresis techniques and supported by molecular docking into the DNA major groove. Antiproliferative effect on the normal human lung fibroblast cell line MRC5 has also been evaluated in order to determine therapeutic potential of Au1-5 and Ag1-5 complexes. Since the investigated gold(III) complexes showed much lower negative effects on the viability of the MRC5 cell line than their silver(I) analogues and slightly lower antimicrobial activity against the investigated strains, the combination approach to improve their pharmacological profiles was applied. Synergistic antimicrobial effect and the selectivity index of 10 were achieved for the selected gold(III)/silver(I) complexes mixtures, as well as higher P. aeruginosa PAO1 biofilm disruption activity, and improved toxicity profile towards zebrafish embryos, in comparison to the single complexes. To the best of our knowledge, this is the first report on synergistic activity of gold(III)/silver(I) complexes mixtures and it could have an impact on development of new combination therapy methods for the treatment of multi-resistant bacterial infections.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - A comparative antimicrobial and toxicological study of gold(III) and silver(I) complexes with aromatic nitrogen-containing heterocycles: synergistic activity and improved selectivity index of Au(III)/Ag(I) complexes mixture
EP  - 13206
IS  - 16
SP  - 13193
VL  - 6
DO  - 10.1039/c5ra26002g
ER  - 

@article{
author = "Savić, Nada D. and Milivojević, Dušan and Glišić, Biljana and Ilić-Tomić, Tatjana and Veselinović, Jovana and Pavić, Aleksandar and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Djuran, Milos I.",
year = "2016",
abstract = "Five aromatic nitrogen-containing heterocycles, pyridazine (pydz, 1), pyrimidine (pm, 2), pyrazine (pz, 3), quinoxaline (qx, 4) and phenazine (phz, 5) have been used for the synthesis of gold(III) and silver(I) complexes. In contrast to the mononuclear Au1-5 complexes all having square-planar geometry, the corresponding Ag1-5 complexes have been found to be polynuclear and of different geometries. Complexes Au1-5 and Ag1-5, along with K[AuCl4], AgNO3 and N-heterocyclic ligands used for their synthesis, were evaluated by in vitro antimicrobial studies against a panel of microbial strains that lead to many skin and soft tissue, respiratory, wound and nosocomial infections. All tested complexes exhibited excellent to good antibacterial activity with minimal inhibitory (MIC) values in the range of 2.5 to 100 mu g mL(-1) against the investigated strains. The complexes were particularly efficient against pathogenic Pseudomonas aeruginosa (MIC = 2.5-30 mu g mL(-1)) and had a marked ability to disrupt clinically relevant biofilms of strains with high inherent resistance to antibiotics. Moreover, the Au1-4 and Ag1-5 complexes exhibited pronounced ability to competitively intercalate double stranded genomic DNA of P. aeruginosa, which was demonstrated by gel electrophoresis techniques and supported by molecular docking into the DNA major groove. Antiproliferative effect on the normal human lung fibroblast cell line MRC5 has also been evaluated in order to determine therapeutic potential of Au1-5 and Ag1-5 complexes. Since the investigated gold(III) complexes showed much lower negative effects on the viability of the MRC5 cell line than their silver(I) analogues and slightly lower antimicrobial activity against the investigated strains, the combination approach to improve their pharmacological profiles was applied. Synergistic antimicrobial effect and the selectivity index of 10 were achieved for the selected gold(III)/silver(I) complexes mixtures, as well as higher P. aeruginosa PAO1 biofilm disruption activity, and improved toxicity profile towards zebrafish embryos, in comparison to the single complexes. To the best of our knowledge, this is the first report on synergistic activity of gold(III)/silver(I) complexes mixtures and it could have an impact on development of new combination therapy methods for the treatment of multi-resistant bacterial infections.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "A comparative antimicrobial and toxicological study of gold(III) and silver(I) complexes with aromatic nitrogen-containing heterocycles: synergistic activity and improved selectivity index of Au(III)/Ag(I) complexes mixture",
pages = "13206-13193",
number = "16",
volume = "6",
doi = "10.1039/c5ra26002g"
}

Savić, N. D., Milivojević, D., Glišić, B., Ilić-Tomić, T., Veselinović, J., Pavić, A., Vasiljević, B., Nikodinović-Runić, J.,& Djuran, M. I.. (2016). A comparative antimicrobial and toxicological study of gold(III) and silver(I) complexes with aromatic nitrogen-containing heterocycles: synergistic activity and improved selectivity index of Au(III)/Ag(I) complexes mixture. in RSC Advances
Royal Soc Chemistry, Cambridge., 6(16), 13193-13206.
https://doi.org/10.1039/c5ra26002g

Savić ND, Milivojević D, Glišić B, Ilić-Tomić T, Veselinović J, Pavić A, Vasiljević B, Nikodinović-Runić J, Djuran MI. A comparative antimicrobial and toxicological study of gold(III) and silver(I) complexes with aromatic nitrogen-containing heterocycles: synergistic activity and improved selectivity index of Au(III)/Ag(I) complexes mixture. in RSC Advances. 2016;6(16):13193-13206.
doi:10.1039/c5ra26002g .

Savić, Nada D., Milivojević, Dušan, Glišić, Biljana, Ilić-Tomić, Tatjana, Veselinović, Jovana, Pavić, Aleksandar, Vasiljević, Branka, Nikodinović-Runić, Jasmina, Djuran, Milos I., "A comparative antimicrobial and toxicological study of gold(III) and silver(I) complexes with aromatic nitrogen-containing heterocycles: synergistic activity and improved selectivity index of Au(III)/Ag(I) complexes mixture" in RSC Advances, 6, no. 16 (2016):13193-13206,
https://doi.org/10.1039/c5ra26002g . .

TY  - JOUR
AU  - Milovanović, Jelena
AU  - Škaro Bogojević, Sanja
AU  - Šenerović, Lidija
AU  - Vasiljević, Branka
AU  - Guzik, Maciej
AU  - Kenny, Shane T.
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Kevin 
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/974
AB  - A library of 18 different compounds was synthesized starting from (R)-3-hydroxyoctanoic acid which is derived from the bacterial polymer polyhydroxyalkanoate (PHA). Ten derivatives, including halo and unsaturated methyl and benzyl esters, were synthesized and characterized for the first time. Given that (R)-3-hydroxyalkanoic acids are known to have biological activity, the new compounds were evaluated for antimicrobial activity and in vitro antiproliferative effect with mammalian cell lines. The presence of the carboxylic group was essential for the antimicrobial activity, with minimal inhibitory concentrations against a panel of bacteria (Gram-positive and Gram-negative) and fungi (Candida albicans and Microsporum gypseum) in the range 2.8-7.0 mM and 0.1-6.3 mM, respectively. 3-Halogenated octanoic acids exhibited the ability to inhibit C. albicans hyphae formation. In addition, (R)-3-hydroxyoctanoic and (E)-oct-2-enoic acids inhibited quorum sensing-regulated pyocyanin production in the opportunistic pathogen Pseudomonas aeruginosa PAO1. Generally, derivatives did not inhibit mammalian cell proliferation even at 3-mM concentrations, while only (E)-oct-2-enoic and 3-oxooctanoic acid had IC50 values of 1.7 and 1.6 mM with the human lung fibroblast cell line.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds
EP  - 172
IS  - 1
SP  - 161
VL  - 100
DO  - 10.1007/s00253-015-6984-4
ER  - 

@article{
author = "Milovanović, Jelena and Škaro Bogojević, Sanja and Šenerović, Lidija and Vasiljević, Branka and Guzik, Maciej and Kenny, Shane T. and Maslak, Veselin and Nikodinović-Runić, Jasmina and O'Connor, Kevin ",
year = "2016",
abstract = "A library of 18 different compounds was synthesized starting from (R)-3-hydroxyoctanoic acid which is derived from the bacterial polymer polyhydroxyalkanoate (PHA). Ten derivatives, including halo and unsaturated methyl and benzyl esters, were synthesized and characterized for the first time. Given that (R)-3-hydroxyalkanoic acids are known to have biological activity, the new compounds were evaluated for antimicrobial activity and in vitro antiproliferative effect with mammalian cell lines. The presence of the carboxylic group was essential for the antimicrobial activity, with minimal inhibitory concentrations against a panel of bacteria (Gram-positive and Gram-negative) and fungi (Candida albicans and Microsporum gypseum) in the range 2.8-7.0 mM and 0.1-6.3 mM, respectively. 3-Halogenated octanoic acids exhibited the ability to inhibit C. albicans hyphae formation. In addition, (R)-3-hydroxyoctanoic and (E)-oct-2-enoic acids inhibited quorum sensing-regulated pyocyanin production in the opportunistic pathogen Pseudomonas aeruginosa PAO1. Generally, derivatives did not inhibit mammalian cell proliferation even at 3-mM concentrations, while only (E)-oct-2-enoic and 3-oxooctanoic acid had IC50 values of 1.7 and 1.6 mM with the human lung fibroblast cell line.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds",
pages = "172-161",
number = "1",
volume = "100",
doi = "10.1007/s00253-015-6984-4"
}

Milovanović, J., Škaro Bogojević, S., Šenerović, L., Vasiljević, B., Guzik, M., Kenny, S. T., Maslak, V., Nikodinović-Runić, J.,& O'Connor, K.. (2016). Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds. in Applied Microbiology and Biotechnology
Springer, New York., 100(1), 161-172.
https://doi.org/10.1007/s00253-015-6984-4

Milovanović J, Škaro Bogojević S, Šenerović L, Vasiljević B, Guzik M, Kenny ST, Maslak V, Nikodinović-Runić J, O'Connor K. Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds. in Applied Microbiology and Biotechnology. 2016;100(1):161-172.
doi:10.1007/s00253-015-6984-4 .

Milovanović, Jelena, Škaro Bogojević, Sanja, Šenerović, Lidija, Vasiljević, Branka, Guzik, Maciej, Kenny, Shane T., Maslak, Veselin, Nikodinović-Runić, Jasmina, O'Connor, Kevin , "Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds" in Applied Microbiology and Biotechnology, 100, no. 1 (2016):161-172,
https://doi.org/10.1007/s00253-015-6984-4 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Zivković, S.
AU  - Pekmezović, M.
AU  - Stanković, Nada
AU  - Vojnović, Sandra
AU  - Vasiljević, Branka
AU  - Šenerović, Lidija
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/876
AB  - AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin
EP  - 1434
IS  - 6
SP  - 1426
VL  - 118
DO  - 10.1111/jam.12811
ER  - 

@article{
author = "Milisavljević, Mira and Zivković, S. and Pekmezović, M. and Stanković, Nada and Vojnović, Sandra and Vasiljević, Branka and Šenerović, Lidija",
year = "2015",
abstract = "AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin",
pages = "1434-1426",
number = "6",
volume = "118",
doi = "10.1111/jam.12811"
}

Milisavljević, M., Zivković, S., Pekmezović, M., Stanković, N., Vojnović, S., Vasiljević, B.,& Šenerović, L.. (2015). Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology
Wiley, Hoboken., 118(6), 1426-1434.
https://doi.org/10.1111/jam.12811

Milisavljević M, Zivković S, Pekmezović M, Stanković N, Vojnović S, Vasiljević B, Šenerović L. Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology. 2015;118(6):1426-1434.
doi:10.1111/jam.12811 .

Milisavljević, Mira, Zivković, S., Pekmezović, M., Stanković, Nada, Vojnović, Sandra, Vasiljević, Branka, Šenerović, Lidija, "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin" in Journal of Applied Microbiology, 118, no. 6 (2015):1426-1434,
https://doi.org/10.1111/jam.12811 . .

TY  - JOUR
AU  - Ilić-Tomić, Tatjana
AU  - Gencić, Marija S.
AU  - Zivković, Milena Z.
AU  - Vasiljević, Branka
AU  - Đokić, Lidija
AU  - Nikodinović-Runić, Jasmina
AU  - Radulović, Niko S.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/875
AB  - Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 A degrees C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C-7-C-30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10
EP  - 4833
IS  - 11
SP  - 4815
VL  - 99
DO  - 10.1007/s00253-014-6364-5
ER  - 

@article{
author = "Ilić-Tomić, Tatjana and Gencić, Marija S. and Zivković, Milena Z. and Vasiljević, Branka and Đokić, Lidija and Nikodinović-Runić, Jasmina and Radulović, Niko S.",
year = "2015",
abstract = "Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 A degrees C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C-7-C-30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10",
pages = "4833-4815",
number = "11",
volume = "99",
doi = "10.1007/s00253-014-6364-5"
}

Ilić-Tomić, T., Gencić, M. S., Zivković, M. Z., Vasiljević, B., Đokić, L., Nikodinović-Runić, J.,& Radulović, N. S.. (2015). Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10. in Applied Microbiology and Biotechnology
Springer, New York., 99(11), 4815-4833.
https://doi.org/10.1007/s00253-014-6364-5

Ilić-Tomić T, Gencić MS, Zivković MZ, Vasiljević B, Đokić L, Nikodinović-Runić J, Radulović NS. Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10. in Applied Microbiology and Biotechnology. 2015;99(11):4815-4833.
doi:10.1007/s00253-014-6364-5 .

Ilić-Tomić, Tatjana, Gencić, Marija S., Zivković, Milena Z., Vasiljević, Branka, Đokić, Lidija, Nikodinović-Runić, Jasmina, Radulović, Niko S., "Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10" in Applied Microbiology and Biotechnology, 99, no. 11 (2015):4815-4833,
https://doi.org/10.1007/s00253-014-6364-5 . .

TY  - JOUR
AU  - Stanković, Srdan
AU  - Morić, Ivana
AU  - Pavić, Aleksandar
AU  - Vojnović, Sandra
AU  - Vasiljević, Branka
AU  - Cvetković, Vladica
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/855
AB  - Bioleaching of samples taken from depths of 10, 15, and 20 m from old flotation tailings of the Copper Mine Bor was conducted in shaken flasks using the extremely acidic water of Lake Robule as lixiviant. The yields of copper after five weeks of the bioleaching experiments were 68.34 +/- 1.21 % for the 15-m sample, 72.57 +/- 0.57 % for the 20-m sample and 97.78 +/- 5.50 % for the 10-m sample. The obtained results were compared to the results of acid leaching of the same samples and it was concluded that bioleaching was generally more efficient for the treatment of samples taken from depths of 10 and 20 m. The content of pyrite in the 20-in sample, which contained the highest amount of this mineral, was reduced after bioleaching. The benefits of this approach are recovery of substantial amounts of copper, reducing the environmental impact of flotation tailings and the application of abundant and free water from the acidic Robule Lake as lixiviant. The obtained results showed that bioleaching could be more efficient than acid leaching for copper extraction from flotation tailings with higher sulfide contents.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Bioleaching of copper from samples of old flotation tailings (Copper Mine Bor, Serbia)
EP  - 405
IS  - 3
SP  - 391
VL  - 80
DO  - 10.2298/JSC140411097S
ER  - 

@article{
author = "Stanković, Srdan and Morić, Ivana and Pavić, Aleksandar and Vojnović, Sandra and Vasiljević, Branka and Cvetković, Vladica",
year = "2015",
abstract = "Bioleaching of samples taken from depths of 10, 15, and 20 m from old flotation tailings of the Copper Mine Bor was conducted in shaken flasks using the extremely acidic water of Lake Robule as lixiviant. The yields of copper after five weeks of the bioleaching experiments were 68.34 +/- 1.21 % for the 15-m sample, 72.57 +/- 0.57 % for the 20-m sample and 97.78 +/- 5.50 % for the 10-m sample. The obtained results were compared to the results of acid leaching of the same samples and it was concluded that bioleaching was generally more efficient for the treatment of samples taken from depths of 10 and 20 m. The content of pyrite in the 20-in sample, which contained the highest amount of this mineral, was reduced after bioleaching. The benefits of this approach are recovery of substantial amounts of copper, reducing the environmental impact of flotation tailings and the application of abundant and free water from the acidic Robule Lake as lixiviant. The obtained results showed that bioleaching could be more efficient than acid leaching for copper extraction from flotation tailings with higher sulfide contents.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Bioleaching of copper from samples of old flotation tailings (Copper Mine Bor, Serbia)",
pages = "405-391",
number = "3",
volume = "80",
doi = "10.2298/JSC140411097S"
}

Stanković, S., Morić, I., Pavić, A., Vojnović, S., Vasiljević, B.,& Cvetković, V.. (2015). Bioleaching of copper from samples of old flotation tailings (Copper Mine Bor, Serbia). in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 80(3), 391-405.
https://doi.org/10.2298/JSC140411097S

Stanković S, Morić I, Pavić A, Vojnović S, Vasiljević B, Cvetković V. Bioleaching of copper from samples of old flotation tailings (Copper Mine Bor, Serbia). in Journal of the Serbian Chemical Society. 2015;80(3):391-405.
doi:10.2298/JSC140411097S .

Stanković, Srdan, Morić, Ivana, Pavić, Aleksandar, Vojnović, Sandra, Vasiljević, Branka, Cvetković, Vladica, "Bioleaching of copper from samples of old flotation tailings (Copper Mine Bor, Serbia)" in Journal of the Serbian Chemical Society, 80, no. 3 (2015):391-405,
https://doi.org/10.2298/JSC140411097S . .

TY  - JOUR
AU  - Đokić, Lidija
AU  - Spasić, Jelena
AU  - Jeremić, Sanja
AU  - Vasiljević, Branka
AU  - Prodanović, Olivera
AU  - Prodanović, Radivoje
AU  - Nikodinović-Runić, Jasmina
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/841
AB  - The enzyme 4-oxalocrotonate tautomerase (4-OT) encoded by the xylH gene is a part of the degradation pathway of aromatic compounds in Pseudomonas putida mt-2. 4-OT was described to catalyze Michael-type addition of acetaldehyde to beta-nitrostyrene, and the whole cell system based on recombinantly expressed 4-OT has been developed previously. In this study biocatalytic process based on Escherichia coli whole cells expressing 4-OT was significantly improved using immobilization and ex situ product recovery strategies. Whole cell immobilization in alginate beads was applied in biocatalytic production of 4-nitro-3-phenyl-butanal from beta-nitrostyrene and acetaldehyde. Immobilized biocatalyst showed wider pH activity range and could tolerate twofold higher initial concentrations of substrate in comparison to the free whole cell biocatalyst. Beads retained their initial activity over 10 consecutive biotransformations of the model reaction and remained suitable for the repetitive use with 85 % of the initial activity after two months of storage. Bioprocess was further improved by utilizing Amberlite XAD-2 hydrophobic resin for the product recovery. With this modification, the amount of organic solvent was reduced 40-fold in comparison to previously reported method making this biocatalytic process greener.
PB  - Springer, New York
T2  - Bioprocess and Biosystems Engineering
T1  - Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene
EP  - 2395
IS  - 12
SP  - 2389
VL  - 38
DO  - 10.1007/s00449-015-1474-8
ER  - 

@article{
author = "Đokić, Lidija and Spasić, Jelena and Jeremić, Sanja and Vasiljević, Branka and Prodanović, Olivera and Prodanović, Radivoje and Nikodinović-Runić, Jasmina",
year = "2015",
abstract = "The enzyme 4-oxalocrotonate tautomerase (4-OT) encoded by the xylH gene is a part of the degradation pathway of aromatic compounds in Pseudomonas putida mt-2. 4-OT was described to catalyze Michael-type addition of acetaldehyde to beta-nitrostyrene, and the whole cell system based on recombinantly expressed 4-OT has been developed previously. In this study biocatalytic process based on Escherichia coli whole cells expressing 4-OT was significantly improved using immobilization and ex situ product recovery strategies. Whole cell immobilization in alginate beads was applied in biocatalytic production of 4-nitro-3-phenyl-butanal from beta-nitrostyrene and acetaldehyde. Immobilized biocatalyst showed wider pH activity range and could tolerate twofold higher initial concentrations of substrate in comparison to the free whole cell biocatalyst. Beads retained their initial activity over 10 consecutive biotransformations of the model reaction and remained suitable for the repetitive use with 85 % of the initial activity after two months of storage. Bioprocess was further improved by utilizing Amberlite XAD-2 hydrophobic resin for the product recovery. With this modification, the amount of organic solvent was reduced 40-fold in comparison to previously reported method making this biocatalytic process greener.",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene",
pages = "2395-2389",
number = "12",
volume = "38",
doi = "10.1007/s00449-015-1474-8"
}

Đokić, L., Spasić, J., Jeremić, S., Vasiljević, B., Prodanović, O., Prodanović, R.,& Nikodinović-Runić, J.. (2015). Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene. in Bioprocess and Biosystems Engineering
Springer, New York., 38(12), 2389-2395.
https://doi.org/10.1007/s00449-015-1474-8

Đokić L, Spasić J, Jeremić S, Vasiljević B, Prodanović O, Prodanović R, Nikodinović-Runić J. Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene. in Bioprocess and Biosystems Engineering. 2015;38(12):2389-2395.
doi:10.1007/s00449-015-1474-8 .

Đokić, Lidija, Spasić, Jelena, Jeremić, Sanja, Vasiljević, Branka, Prodanović, Olivera, Prodanović, Radivoje, Nikodinović-Runić, Jasmina, "Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene" in Bioprocess and Biosystems Engineering, 38, no. 12 (2015):2389-2395,
https://doi.org/10.1007/s00449-015-1474-8 . .

TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Ilić-Tomić, Tatjana
AU  - Pacevski, Aleksandar
AU  - Nedeljković, Tatjana
AU  - Vasiljević, Branka
AU  - Morić, Ivana
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/851
AB  - Cultivable bacteria colonizing deteriorated modern painting on canvas were identified in order to evaluate their potential to deteriorate organic and inorganic painting's constituents. Different sampling and cultivation strategies enabled isolation of bacteria belonging to nine genera of Firmicutes, Proteobacteria, and Actinobacteria phyla. Overall predominant bacteria were species of Bacillus (51%) and Staphylococcus (36%) genera. Representatives of six different genera (Staphylococcus, Acinetobacter, Agrococcus, Janibacter, Rhodococcus, and Stenotrophomonas) were isolated for the first time from deteriorated canvas. Almost all isolated bacteria produced proteases, esterases, and lipases, which may be involved in deterioration of painting's binders and media. Bacteria expressing endocellulase were reported. Selected bacterial isolates were tested for ability to deteriorate six pigments. All tested isolates were able to grow in the presence of Ivory black, Red and Yellow ochre, as a sole source of phosphate and iron, inducing their fading. The majority of isolates induced solubilization of Zinc white and Cobalt deep green. Cadmium red pigment that inhibited the growth of a half of isolates proven to be the most toxic pigment. Isolated bacteria were equipped with all required metabolic prerequisites in order to pose a threat to the painting as a whole.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Biodeterioration & Biodegradation
T1  - Diversity and biodeteriorative potential of bacterial isolates from deteriorated modern combined-technique canvas painting
EP  - 50
SP  - 40
VL  - 97
DO  - 10.1016/j.ibiod.2014.11.012
ER  - 

@article{
author = "Pavić, Aleksandar and Ilić-Tomić, Tatjana and Pacevski, Aleksandar and Nedeljković, Tatjana and Vasiljević, Branka and Morić, Ivana",
year = "2015",
abstract = "Cultivable bacteria colonizing deteriorated modern painting on canvas were identified in order to evaluate their potential to deteriorate organic and inorganic painting's constituents. Different sampling and cultivation strategies enabled isolation of bacteria belonging to nine genera of Firmicutes, Proteobacteria, and Actinobacteria phyla. Overall predominant bacteria were species of Bacillus (51%) and Staphylococcus (36%) genera. Representatives of six different genera (Staphylococcus, Acinetobacter, Agrococcus, Janibacter, Rhodococcus, and Stenotrophomonas) were isolated for the first time from deteriorated canvas. Almost all isolated bacteria produced proteases, esterases, and lipases, which may be involved in deterioration of painting's binders and media. Bacteria expressing endocellulase were reported. Selected bacterial isolates were tested for ability to deteriorate six pigments. All tested isolates were able to grow in the presence of Ivory black, Red and Yellow ochre, as a sole source of phosphate and iron, inducing their fading. The majority of isolates induced solubilization of Zinc white and Cobalt deep green. Cadmium red pigment that inhibited the growth of a half of isolates proven to be the most toxic pigment. Isolated bacteria were equipped with all required metabolic prerequisites in order to pose a threat to the painting as a whole.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Biodeterioration & Biodegradation",
title = "Diversity and biodeteriorative potential of bacterial isolates from deteriorated modern combined-technique canvas painting",
pages = "50-40",
volume = "97",
doi = "10.1016/j.ibiod.2014.11.012"
}

Pavić, A., Ilić-Tomić, T., Pacevski, A., Nedeljković, T., Vasiljević, B.,& Morić, I.. (2015). Diversity and biodeteriorative potential of bacterial isolates from deteriorated modern combined-technique canvas painting. in International Biodeterioration & Biodegradation
Elsevier Sci Ltd, Oxford., 97, 40-50.
https://doi.org/10.1016/j.ibiod.2014.11.012

Pavić A, Ilić-Tomić T, Pacevski A, Nedeljković T, Vasiljević B, Morić I. Diversity and biodeteriorative potential of bacterial isolates from deteriorated modern combined-technique canvas painting. in International Biodeterioration & Biodegradation. 2015;97:40-50.
doi:10.1016/j.ibiod.2014.11.012 .

Pavić, Aleksandar, Ilić-Tomić, Tatjana, Pacevski, Aleksandar, Nedeljković, Tatjana, Vasiljević, Branka, Morić, Ivana, "Diversity and biodeteriorative potential of bacterial isolates from deteriorated modern combined-technique canvas painting" in International Biodeterioration & Biodegradation, 97 (2015):40-50,
https://doi.org/10.1016/j.ibiod.2014.11.012 . .