TY  - JOUR
AU  - Rakonjac, Marijana
AU  - Cuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Simeunović, Ivana
AU  - Kostić, Jovana
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
PY  - 2024
UR  - https://www.mdpi.com/2227-9067/11/4/489
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2362
AB  - 22q11.2 deletion syndrome (22q11.2DS), the most frequent microdeletion syndrome in humans, is related to a high risk of developing neurodevelopmental disorders. About 95% of patients with 22q11.2DS have speech and language impairments. Global articulation, story generation, and verbal memory tests were applied to compare articulatory characteristics of speech sounds, spontaneous language abilities, and immediate verbal memory between four groups of Serbian-speaking children: patients with 22q11.2DS, children with clinical presentation of 22q11.2DS that do not have the microdeletion, children with non-syndromic congenital heart defects, and their peers with typical speech–sound development. The obtained results showed that children with this microdeletion have impaired articulation skills and expressive language abilities. However, we did not observe weaker receptive language skills and immediate verbal memory compared to healthy controls. Children with 22q11.2DS should be considered a risk category for the development of speech–sound pathology and expressive language abilities. Since speech intelligibility is an instrument of cognition and adequate peer socialization, and language impairment in school-aged children with 22q11DS might be an indicator of increased risk for later psychotic symptoms, patients with 22q11.2 microdeletion should be included in a program of early stimulation of speech–language development immediately after diagnosis is established.
PB  - MDPI
T2  - Children
T2  - Children
T1  - Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion
IS  - 4
SP  - 489
VL  - 11
DO  - 10.3390/children11040489
ER  - 

@article{
author = "Rakonjac, Marijana and Cuturilo, Goran and Kovačević-Grujičić, Nataša and Simeunović, Ivana and Kostić, Jovana and Stevanović, Milena and Drakulić, Danijela",
year = "2024",
abstract = "22q11.2 deletion syndrome (22q11.2DS), the most frequent microdeletion syndrome in humans, is related to a high risk of developing neurodevelopmental disorders. About 95% of patients with 22q11.2DS have speech and language impairments. Global articulation, story generation, and verbal memory tests were applied to compare articulatory characteristics of speech sounds, spontaneous language abilities, and immediate verbal memory between four groups of Serbian-speaking children: patients with 22q11.2DS, children with clinical presentation of 22q11.2DS that do not have the microdeletion, children with non-syndromic congenital heart defects, and their peers with typical speech–sound development. The obtained results showed that children with this microdeletion have impaired articulation skills and expressive language abilities. However, we did not observe weaker receptive language skills and immediate verbal memory compared to healthy controls. Children with 22q11.2DS should be considered a risk category for the development of speech–sound pathology and expressive language abilities. Since speech intelligibility is an instrument of cognition and adequate peer socialization, and language impairment in school-aged children with 22q11DS might be an indicator of increased risk for later psychotic symptoms, patients with 22q11.2 microdeletion should be included in a program of early stimulation of speech–language development immediately after diagnosis is established.",
publisher = "MDPI",
journal = "Children, Children",
title = "Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion",
number = "4",
pages = "489",
volume = "11",
doi = "10.3390/children11040489"
}

Rakonjac, M., Cuturilo, G., Kovačević-Grujičić, N., Simeunović, I., Kostić, J., Stevanović, M.,& Drakulić, D.. (2024). Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion. in Children
MDPI., 11(4), 489.
https://doi.org/10.3390/children11040489

Rakonjac M, Cuturilo G, Kovačević-Grujičić N, Simeunović I, Kostić J, Stevanović M, Drakulić D. Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion. in Children. 2024;11(4):489.
doi:10.3390/children11040489 .

Rakonjac, Marijana, Cuturilo, Goran, Kovačević-Grujičić, Nataša, Simeunović, Ivana, Kostić, Jovana, Stevanović, Milena, Drakulić, Danijela, "Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion" in Children, 11, no. 4 (2024):489,
https://doi.org/10.3390/children11040489 . .

TY  - CONF
AU  - Drakulić, Danijela
AU  - Petrakis, Spyros
AU  - Harwood, Adrian J.
AU  - Linden, David
AU  - Lazić, Andrijana
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1325
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2361
AB  - Neurodevelopmental disorders (NDDs) are caused by alterations in early brain development. They are a group of geographically dispersed, complex and heterogeneous disorders that give rise to the psychiatric conditions such as autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. In order to build global research activity for study of NDDs, the main goals of the Twinning project STREAMLINE are to enhanced strategic networking and reinforce research and innovation potential of the Institute of Molecular Genetics and Genetic Engineering, University of Belgrade (IMGGE) in order to develop IMGGE as a high capacity hub for research of NDDs in the Western Balkans. This will be achieved by twinning IMGGE with three top-class research institutions in Europe (Cardiff University, University of Maastricht and Centre for Research and Technology Hellas) with an exceptional expertise in the stem cells based research of NDDs, -OMICS technologies, bioinformatics data analysis and drug testing and through staff exchanges, training, and organization of summer schools, Industry Open Days, symposia and workshops.
C3  - Hemijska industrija (Chemical Industry)
T1  - STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region
EP  - 78
IS  - 1S
SP  - 78
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2361
ER  - 

@conference{
author = "Drakulić, Danijela and Petrakis, Spyros and Harwood, Adrian J. and Linden, David and Lazić, Andrijana and Kovačević-Grujičić, Nataša and Stevanović, Milena",
year = "2024",
abstract = "Neurodevelopmental disorders (NDDs) are caused by alterations in early brain development. They are a group of geographically dispersed, complex and heterogeneous disorders that give rise to the psychiatric conditions such as autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. In order to build global research activity for study of NDDs, the main goals of the Twinning project STREAMLINE are to enhanced strategic networking and reinforce research and innovation potential of the Institute of Molecular Genetics and Genetic Engineering, University of Belgrade (IMGGE) in order to develop IMGGE as a high capacity hub for research of NDDs in the Western Balkans. This will be achieved by twinning IMGGE with three top-class research institutions in Europe (Cardiff University, University of Maastricht and Centre for Research and Technology Hellas) with an exceptional expertise in the stem cells based research of NDDs, -OMICS technologies, bioinformatics data analysis and drug testing and through staff exchanges, training, and organization of summer schools, Industry Open Days, symposia and workshops.",
journal = "Hemijska industrija (Chemical Industry)",
title = "STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region",
pages = "78-78",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2361"
}

Drakulić, D., Petrakis, S., Harwood, A. J., Linden, D., Lazić, A., Kovačević-Grujičić, N.,& Stevanović, M.. (2024). STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region. in Hemijska industrija (Chemical Industry), 78(1S), 78-78.
https://hdl.handle.net/21.15107/rcub_imagine_2361

Drakulić D, Petrakis S, Harwood AJ, Linden D, Lazić A, Kovačević-Grujičić N, Stevanović M. STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region. in Hemijska industrija (Chemical Industry). 2024;78(1S):78-78.
https://hdl.handle.net/21.15107/rcub_imagine_2361 .

Drakulić, Danijela, Petrakis, Spyros, Harwood, Adrian J., Linden, David, Lazić, Andrijana, Kovačević-Grujičić, Nataša, Stevanović, Milena, "STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):78-78,
https://hdl.handle.net/21.15107/rcub_imagine_2361 .

TY  - JOUR
AU  - Kloska, Anna
AU  - Giełczyk, Agata
AU  - Grzybowski, Tomasz
AU  - Płoski, Rafał
AU  - Kloska, Sylwester M.
AU  - Marciniak, Tomasz
AU  - Pałczyński, Krzysztof
AU  - Rogalla-Ładniak, Urszula
AU  - Malyarchuk, Boris A.
AU  - Derenko, Miroslava V.
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Davidović, Slobodan
AU  - Spólnicka, Magdalena
AU  - Zubańska, Magdalena
AU  - Woźniak, Marcin
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/20/15095
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2171
AB  - Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.
T2  - International Journal of Molecular Sciences
T1  - A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe
IS  - 20
SP  - 15095
VL  - 24
DO  - 10.3390/ijms242015095
ER  - 

@article{
author = "Kloska, Anna and Giełczyk, Agata and Grzybowski, Tomasz and Płoski, Rafał and Kloska, Sylwester M. and Marciniak, Tomasz and Pałczyński, Krzysztof and Rogalla-Ładniak, Urszula and Malyarchuk, Boris A. and Derenko, Miroslava V. and Kovačević-Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela and Davidović, Slobodan and Spólnicka, Magdalena and Zubańska, Magdalena and Woźniak, Marcin",
year = "2023",
abstract = "Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.",
journal = "International Journal of Molecular Sciences",
title = "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe",
number = "20",
pages = "15095",
volume = "24",
doi = "10.3390/ijms242015095"
}

Kloska, A., Giełczyk, A., Grzybowski, T., Płoski, R., Kloska, S. M., Marciniak, T., Pałczyński, K., Rogalla-Ładniak, U., Malyarchuk, B. A., Derenko, M. V., Kovačević-Grujičić, N., Stevanović, M., Drakulić, D., Davidović, S., Spólnicka, M., Zubańska, M.,& Woźniak, M.. (2023). A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences, 24(20), 15095.
https://doi.org/10.3390/ijms242015095

Kloska A, Giełczyk A, Grzybowski T, Płoski R, Kloska SM, Marciniak T, Pałczyński K, Rogalla-Ładniak U, Malyarchuk BA, Derenko MV, Kovačević-Grujičić N, Stevanović M, Drakulić D, Davidović S, Spólnicka M, Zubańska M, Woźniak M. A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences. 2023;24(20):15095.
doi:10.3390/ijms242015095 .

Kloska, Anna, Giełczyk, Agata, Grzybowski, Tomasz, Płoski, Rafał, Kloska, Sylwester M., Marciniak, Tomasz, Pałczyński, Krzysztof, Rogalla-Ładniak, Urszula, Malyarchuk, Boris A., Derenko, Miroslava V., Kovačević-Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, Davidović, Slobodan, Spólnicka, Magdalena, Zubańska, Magdalena, Woźniak, Marcin, "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe" in International Journal of Molecular Sciences, 24, no. 20 (2023):15095,
https://doi.org/10.3390/ijms242015095 . .

TY  - CHAP
AU  - Davidović, Slobodan
AU  - Aleksić, Jelena
AU  - Stevanović, Milena
AU  - Kovačević Grujičić, Nataša
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2245
AB  - Mitohondrijska DNK (mtDNK) se odlikuje nizom osobina koje je čine pogodnom za istraživanja evolutivne
istorije ljudskih populacija koja se zasniva na molekularnim markerima ženske linije nasleđivanja. Tokom
poslednje decenije publikovano je više naučnih radova u kojima je analizirana varijabilnost mtDNK u populaciji
Srbije primenom markera različite rezolucije uključujući i kompletne genome. U skladu sa očekivanjima
zasnovanim na istorijskim, arheološkim i drugim izvorima koji govore u prilog veoma kompleksne
istorije populacija na Balkanskom poluostrvu, mtDNK podaci su potvrdili da se srpska populacija odlikuje
visokim nivoom raznovrsnosti mtDNK koji je posledica izuzetno složene dinamike ove populacije tokom
vremena. Današnji mtDNK profil populacije Srbije ne odstupa od matrilinealnog profila karakterističnog
za druge evropske populacije, a genetičke distance pokazuju da ova populacija zauzima centralnu poziciju
unutar grupe južnoslovenskih populacija koje se odlikuju visokom heterogenošću. Srpska populacija
deli najveći procenat mtDNK haplotipova sa geografski bliskim populacijama Balkanskog poluostrva koje
pripadaju južnoslovenskoj grupi, gde su uočeni i potencijalno privatni haplotipovi. Na osnovu filogenetske
i filogeografske analize kompletnih mitogenoma u srpskoj populaciji detektovane su retke mtDNK linije,
karakteristične za druge regione, poput Bliskog istoka (N1b, HV2), istočne Azije (D4) i Afrike (L2a1), kao i
one koje su potencijalno specifične za Balkansko poluostrvo, poput K1a13a1, U4c1b1 i H6a2b. Pored toga,
srpska populacija deli određeni broj mtDNK podhaplogrupa sa istočno- i zapadnoslovenskim populacijama
kao i sa germanskim populacijama severne i srednje Evrope. Istraživanja varijabilnosti mtDNK su pokazala
da se izuzetno velika raznovrsnost mtDNK savremene populacije Srbije može objasniti genetičkim doprinosom
kako slovenskih i germanskih, tako i pre-slovenskih populacija koje su naseljavale Balkansko poluostrvo
pre Velike seobe naroda.
AB  - The mitochondrial DNA (mtDNA) is characterized by a number of features that make it suitable for studying
the evolutionary history of human populations based on molecular markers with the female-specific
line of inheritance. During the last decade, several scientific papers were published in which the mtDNA
variability in the population of Serbia was analyzed using markers of different resolution including complete
mitogenomes. In accordance with expectations based on historical, archaeological and other sources
that speak in favor of a very complex history of populations on the Balkan Peninsula, mtDNA data confirmed
that Serbian population is characterized by a high level of mtDNA diversity, which is a consequence
of the exceptionally complex dynamics of this population over time. Today’s mtDNA profile of the Serbian
population does not differ from the matrilineal landscape characteristic of other European populations,
and according to genetic distances, this population occupies a central position within the group of South-
Slavic populations characterized by high heterogeneity. The Serbian population shares the highest percentage
of mtDNA haplotypes with the geographically close populations of the Balkan Peninsula
belonging to the South-Slavic group, where potentially private haplotypes were also observed. Phylogenetic
and phylogeographic analysis of complete mitogenomes in the Serbian population revealed rare
mtDNA lineages, characteristic of other regions, such as the Middle East (N1b, HV2), East Asia (D4) and
Africa (L2a1), as well as those that are potentially specific for Balkan Peninsula, like K1a13a1, U4c1b1 and
H6a2b. In addition, Serbian population shares a certain number of mtDNA subhaplogroups with East- and
West-Slavic populations as well as with the Germanic populations of Northern and Central Europe. Studies
of mtDNA variability have shown that the exceptionally high mtDNA diversity in contemporary Serbian
population may be associated with the genetic contribution of both Slavic and Germanic, as well as pre-
Slavic populations that inhabited the Balkan Peninsula before the Great Migration.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
T2  - Trendovi u molekularnoj Biologiji
T1  - Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije
T1  - Mitochondrial gene pool variability of the residents of the Republic of Serbia
EP  - 36
IS  - 3
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2245
ER  - 

@inbook{
author = "Davidović, Slobodan and Aleksić, Jelena and Stevanović, Milena and Kovačević Grujičić, Nataša",
year = "2023",
abstract = "Mitohondrijska DNK (mtDNK) se odlikuje nizom osobina koje je čine pogodnom za istraživanja evolutivne
istorije ljudskih populacija koja se zasniva na molekularnim markerima ženske linije nasleđivanja. Tokom
poslednje decenije publikovano je više naučnih radova u kojima je analizirana varijabilnost mtDNK u populaciji
Srbije primenom markera različite rezolucije uključujući i kompletne genome. U skladu sa očekivanjima
zasnovanim na istorijskim, arheološkim i drugim izvorima koji govore u prilog veoma kompleksne
istorije populacija na Balkanskom poluostrvu, mtDNK podaci su potvrdili da se srpska populacija odlikuje
visokim nivoom raznovrsnosti mtDNK koji je posledica izuzetno složene dinamike ove populacije tokom
vremena. Današnji mtDNK profil populacije Srbije ne odstupa od matrilinealnog profila karakterističnog
za druge evropske populacije, a genetičke distance pokazuju da ova populacija zauzima centralnu poziciju
unutar grupe južnoslovenskih populacija koje se odlikuju visokom heterogenošću. Srpska populacija
deli najveći procenat mtDNK haplotipova sa geografski bliskim populacijama Balkanskog poluostrva koje
pripadaju južnoslovenskoj grupi, gde su uočeni i potencijalno privatni haplotipovi. Na osnovu filogenetske
i filogeografske analize kompletnih mitogenoma u srpskoj populaciji detektovane su retke mtDNK linije,
karakteristične za druge regione, poput Bliskog istoka (N1b, HV2), istočne Azije (D4) i Afrike (L2a1), kao i
one koje su potencijalno specifične za Balkansko poluostrvo, poput K1a13a1, U4c1b1 i H6a2b. Pored toga,
srpska populacija deli određeni broj mtDNK podhaplogrupa sa istočno- i zapadnoslovenskim populacijama
kao i sa germanskim populacijama severne i srednje Evrope. Istraživanja varijabilnosti mtDNK su pokazala
da se izuzetno velika raznovrsnost mtDNK savremene populacije Srbije može objasniti genetičkim doprinosom
kako slovenskih i germanskih, tako i pre-slovenskih populacija koje su naseljavale Balkansko poluostrvo
pre Velike seobe naroda., The mitochondrial DNA (mtDNA) is characterized by a number of features that make it suitable for studying
the evolutionary history of human populations based on molecular markers with the female-specific
line of inheritance. During the last decade, several scientific papers were published in which the mtDNA
variability in the population of Serbia was analyzed using markers of different resolution including complete
mitogenomes. In accordance with expectations based on historical, archaeological and other sources
that speak in favor of a very complex history of populations on the Balkan Peninsula, mtDNA data confirmed
that Serbian population is characterized by a high level of mtDNA diversity, which is a consequence
of the exceptionally complex dynamics of this population over time. Today’s mtDNA profile of the Serbian
population does not differ from the matrilineal landscape characteristic of other European populations,
and according to genetic distances, this population occupies a central position within the group of South-
Slavic populations characterized by high heterogeneity. The Serbian population shares the highest percentage
of mtDNA haplotypes with the geographically close populations of the Balkan Peninsula
belonging to the South-Slavic group, where potentially private haplotypes were also observed. Phylogenetic
and phylogeographic analysis of complete mitogenomes in the Serbian population revealed rare
mtDNA lineages, characteristic of other regions, such as the Middle East (N1b, HV2), East Asia (D4) and
Africa (L2a1), as well as those that are potentially specific for Balkan Peninsula, like K1a13a1, U4c1b1 and
H6a2b. In addition, Serbian population shares a certain number of mtDNA subhaplogroups with East- and
West-Slavic populations as well as with the Germanic populations of Northern and Central Europe. Studies
of mtDNA variability have shown that the exceptionally high mtDNA diversity in contemporary Serbian
population may be associated with the genetic contribution of both Slavic and Germanic, as well as pre-
Slavic populations that inhabited the Balkan Peninsula before the Great Migration.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
booktitle = "Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije, Mitochondrial gene pool variability of the residents of the Republic of Serbia",
pages = "36-18",
number = "3",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2245"
}

Davidović, S., Aleksić, J., Stevanović, M.,& Kovačević Grujičić, N.. (2023). Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo.(3), 18-36.
https://hdl.handle.net/21.15107/rcub_imagine_2245

Davidović S, Aleksić J, Stevanović M, Kovačević Grujičić N. Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije. in Trendovi u molekularnoj Biologiji. 2023;(3):18-36.
https://hdl.handle.net/21.15107/rcub_imagine_2245 .

Davidović, Slobodan, Aleksić, Jelena, Stevanović, Milena, Kovačević Grujičić, Nataša, "Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije" in Trendovi u molekularnoj Biologiji, no. 3 (2023):18-36,
https://hdl.handle.net/21.15107/rcub_imagine_2245 .

TY  - CONF
AU  - Balint, Vanda
AU  - Stanisavljević-Ninković, Danijela
AU  - Lazić, Stefan
AU  - Kovačević-Grujičić, Nataša
AU  - Perić, Mina
AU  - Pejić, Jelena
AU  - Mojsin, Marija
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2185
AB  - Astrocytes are the main homeostatic cells in the brain with important roles both in
physiological and pathological conditions. They have a unique ability to become
reactivated in response to different types of brain pathologies, which serves as a
compensatory response that modulates tissue damage and recovery. Also, senescent
astrocytes have profound implications in age-related neurodegenerative disorders. The
molecular mechanisms underlying astrocyte reactivation and senescence are still not
well understood. To investigate the roles of SOX2 and SOX9 transcription factors and
miR-21 in these phenotypic alternations of astroglia, astrocytes derived from NT2/D1
cell line (NT2/A) were used as a model system. Western blot analyses showed that the
expression of both SOX2 and SOX9 decreases during the maturation of NT2/A and
they are re-expressed upon in vitro induced injury. Further modulation of the SOX2
and SOX9 expression will reveal their roles in the regulation of astrocytes
reactivation. Down-regulation of mir-21 in both immature and mature NT2/A by
using the antisense technology, induced the decline in cell proliferation revealed by
Ki67 proliferation marker. Also the premature cellular senescence was induced as
indicated by increase in SA-ß-gal activity and the expression of p21 and p53.
Additionally, in silico analysis predicted many of the genes, previously shown to be
upregulated in senescent astrocytes, as miR-21 targets.
Clarifying the roles of SOX genes and miRNAs in astrocyte reactivation and
senescence would contribute to better understanding of the functions of these cells at
the molecular level, which holds promise for development of new therapeutic
strategies.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells
EP  - 99
SP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2185
ER  - 

@conference{
author = "Balint, Vanda and Stanisavljević-Ninković, Danijela and Lazić, Stefan and Kovačević-Grujičić, Nataša and Perić, Mina and Pejić, Jelena and Mojsin, Marija and Stevanović, Milena and Lazić, Andrijana",
year = "2023",
abstract = "Astrocytes are the main homeostatic cells in the brain with important roles both in
physiological and pathological conditions. They have a unique ability to become
reactivated in response to different types of brain pathologies, which serves as a
compensatory response that modulates tissue damage and recovery. Also, senescent
astrocytes have profound implications in age-related neurodegenerative disorders. The
molecular mechanisms underlying astrocyte reactivation and senescence are still not
well understood. To investigate the roles of SOX2 and SOX9 transcription factors and
miR-21 in these phenotypic alternations of astroglia, astrocytes derived from NT2/D1
cell line (NT2/A) were used as a model system. Western blot analyses showed that the
expression of both SOX2 and SOX9 decreases during the maturation of NT2/A and
they are re-expressed upon in vitro induced injury. Further modulation of the SOX2
and SOX9 expression will reveal their roles in the regulation of astrocytes
reactivation. Down-regulation of mir-21 in both immature and mature NT2/A by
using the antisense technology, induced the decline in cell proliferation revealed by
Ki67 proliferation marker. Also the premature cellular senescence was induced as
indicated by increase in SA-ß-gal activity and the expression of p21 and p53.
Additionally, in silico analysis predicted many of the genes, previously shown to be
upregulated in senescent astrocytes, as miR-21 targets.
Clarifying the roles of SOX genes and miRNAs in astrocyte reactivation and
senescence would contribute to better understanding of the functions of these cells at
the molecular level, which holds promise for development of new therapeutic
strategies.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells",
pages = "99-99",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2185"
}

Balint, V., Stanisavljević-Ninković, D., Lazić, S., Kovačević-Grujičić, N., Perić, M., Pejić, J., Mojsin, M., Stevanović, M.,& Lazić, A.. (2023). The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 99-99.
https://hdl.handle.net/21.15107/rcub_imagine_2185

Balint V, Stanisavljević-Ninković D, Lazić S, Kovačević-Grujičić N, Perić M, Pejić J, Mojsin M, Stevanović M, Lazić A. The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells. in 8th Congress of the Serbian Neuroscience Society. 2023;:99-99.
https://hdl.handle.net/21.15107/rcub_imagine_2185 .

Balint, Vanda, Stanisavljević-Ninković, Danijela, Lazić, Stefan, Kovačević-Grujičić, Nataša, Perić, Mina, Pejić, Jelena, Mojsin, Marija, Stevanović, Milena, Lazić, Andrijana, "The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells" in 8th Congress of the Serbian Neuroscience Society (2023):99-99,
https://hdl.handle.net/21.15107/rcub_imagine_2185 .

TY  - CONF
AU  - Simeunović, Ivana
AU  - Drakulić, Danijela
AU  - Cuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Kostić, Jovana
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2184
AB  - Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD),
intellectual disability (ID), schizophrenia, and bipolar disorder, are caused by
disruption of brain development. They affect approximately 4% of the European
population. However, molecular mechanisms underlying NDDs are still unknown.
One of the syndromes with a high risk for NDDs is 22q11.2 Deletion Syndrome
(22q11.2DS) caused by microdeletion 22q11.2. 22q11.2DS is the most common
microdeletion in humans; approximately, 25% of patients with 22q11.2DS develop
schizophrenia compared to 1% in the general population, while an ID is detected in
approximately 45% of patients and ASD in 14-50% of cases. We analyzed genomic
and clinical findings in our cohort of 35 patients with 22q11.2DS. The majority of
patients have 3 Mb deletion and nine patients have inherited 22q11.2 microdeletion
from their parents. Twenty-one different clinical presentations are revealed in the
cohort with developmental delay detected in about 50% of patients. Approximately
80% of patients have heart malformations, palatal clefts/velopharyngeal insufficiency
was detected in about 30% of them, facial dysmorphism in approximately 80% and
hypocalcemia was seen in about 20% of patients. Here we presented a cohort of
patients with 22q11.2DS which represents a good system for modeling NDDs in vitro.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia
EP  - 98
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2184
ER  - 

@conference{
author = "Simeunović, Ivana and Drakulić, Danijela and Cuturilo, Goran and Kovačević-Grujičić, Nataša and Kostić, Jovana and Stevanović, Milena",
year = "2023",
abstract = "Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD),
intellectual disability (ID), schizophrenia, and bipolar disorder, are caused by
disruption of brain development. They affect approximately 4% of the European
population. However, molecular mechanisms underlying NDDs are still unknown.
One of the syndromes with a high risk for NDDs is 22q11.2 Deletion Syndrome
(22q11.2DS) caused by microdeletion 22q11.2. 22q11.2DS is the most common
microdeletion in humans; approximately, 25% of patients with 22q11.2DS develop
schizophrenia compared to 1% in the general population, while an ID is detected in
approximately 45% of patients and ASD in 14-50% of cases. We analyzed genomic
and clinical findings in our cohort of 35 patients with 22q11.2DS. The majority of
patients have 3 Mb deletion and nine patients have inherited 22q11.2 microdeletion
from their parents. Twenty-one different clinical presentations are revealed in the
cohort with developmental delay detected in about 50% of patients. Approximately
80% of patients have heart malformations, palatal clefts/velopharyngeal insufficiency
was detected in about 30% of them, facial dysmorphism in approximately 80% and
hypocalcemia was seen in about 20% of patients. Here we presented a cohort of
patients with 22q11.2DS which represents a good system for modeling NDDs in vitro.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia",
pages = "98-98",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2184"
}

Simeunović, I., Drakulić, D., Cuturilo, G., Kovačević-Grujičić, N., Kostić, J.,& Stevanović, M.. (2023). Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2184

Simeunović I, Drakulić D, Cuturilo G, Kovačević-Grujičić N, Kostić J, Stevanović M. Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia. in 8th Congress of the Serbian Neuroscience Society. 2023;:98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2184 .

Simeunović, Ivana, Drakulić, Danijela, Cuturilo, Goran, Kovačević-Grujičić, Nataša, Kostić, Jovana, Stevanović, Milena, "Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia" in 8th Congress of the Serbian Neuroscience Society (2023):98-98,
https://hdl.handle.net/21.15107/rcub_imagine_2184 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Drakulić, Danijela
AU  - Cuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Simeunović, Ivana
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2194
AB  - Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD),
schizophrenia, and intellectual disability, are caused by disruption of early brain
development. NDDs represent important public health challenge in modern societies
with prevalence of about 10 to 15% of all births and the tendency of increasing
worldwide. On the other side, treatments of NDDs are focused on symptoms due to
limited understanding of underlying pathophysiological mechanisms. Individuals with
the 22q11.2 duplication syndrome (22q11.2dup), caused by heterozygous 22q11.2
microduplication, have an elevated risk of developing NDDs. Literature data revealed
that ASD is detected in 14-25% of patients with 22q11.2dup while schizophrenia is
less common in these patients than in the general population, suggesting that
22q11.2dup might be protective against schizophrenia. We investigated genomic and
clinical findings in cohort of 8 patients with 22q11.2dup. The majority of patients
have 3Mb duplication. Five patients have 22q11.2 microduplication inherited from
their parents. Other CNVs or SNVs are detected in 5 out of 8 patients. Common
medical anomalies in our cohort of patients include developmental delay, facial
dysmorphism, heart malformations, anomalies of the skeletal system, and anomalies
affecting the eye. Characterization of a cohort of patients with 22q11.2dup is
important since 22q11.2dup represents a powerful model to get insights into the
molecular mechanisms underlying NDDs.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - Genomic and clinical findings in patients with 22q11.2 duplication syndrome
EP  - 97
SP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2194
ER  - 

@conference{
author = "Kostić, Jovana and Drakulić, Danijela and Cuturilo, Goran and Kovačević-Grujičić, Nataša and Simeunović, Ivana and Stevanović, Milena",
year = "2023",
abstract = "Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD),
schizophrenia, and intellectual disability, are caused by disruption of early brain
development. NDDs represent important public health challenge in modern societies
with prevalence of about 10 to 15% of all births and the tendency of increasing
worldwide. On the other side, treatments of NDDs are focused on symptoms due to
limited understanding of underlying pathophysiological mechanisms. Individuals with
the 22q11.2 duplication syndrome (22q11.2dup), caused by heterozygous 22q11.2
microduplication, have an elevated risk of developing NDDs. Literature data revealed
that ASD is detected in 14-25% of patients with 22q11.2dup while schizophrenia is
less common in these patients than in the general population, suggesting that
22q11.2dup might be protective against schizophrenia. We investigated genomic and
clinical findings in cohort of 8 patients with 22q11.2dup. The majority of patients
have 3Mb duplication. Five patients have 22q11.2 microduplication inherited from
their parents. Other CNVs or SNVs are detected in 5 out of 8 patients. Common
medical anomalies in our cohort of patients include developmental delay, facial
dysmorphism, heart malformations, anomalies of the skeletal system, and anomalies
affecting the eye. Characterization of a cohort of patients with 22q11.2dup is
important since 22q11.2dup represents a powerful model to get insights into the
molecular mechanisms underlying NDDs.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "Genomic and clinical findings in patients with 22q11.2 duplication syndrome",
pages = "97-97",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2194"
}

Kostić, J., Drakulić, D., Cuturilo, G., Kovačević-Grujičić, N., Simeunović, I.,& Stevanović, M.. (2023). Genomic and clinical findings in patients with 22q11.2 duplication syndrome. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 97-97.
https://hdl.handle.net/21.15107/rcub_imagine_2194

Kostić J, Drakulić D, Cuturilo G, Kovačević-Grujičić N, Simeunović I, Stevanović M. Genomic and clinical findings in patients with 22q11.2 duplication syndrome. in 8th Congress of the Serbian Neuroscience Society. 2023;:97-97.
https://hdl.handle.net/21.15107/rcub_imagine_2194 .

Kostić, Jovana, Drakulić, Danijela, Cuturilo, Goran, Kovačević-Grujičić, Nataša, Simeunović, Ivana, Stevanović, Milena, "Genomic and clinical findings in patients with 22q11.2 duplication syndrome" in 8th Congress of the Serbian Neuroscience Society (2023):97-97,
https://hdl.handle.net/21.15107/rcub_imagine_2194 .

TY  - CONF
AU  - Simeunović, Ivana
AU  - Čuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Petter, Olena
AU  - Perić, Mina
AU  - Kostić, Jovana
AU  - Harwood J., Adrian
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2138
AB  - Introduction: Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD), intellectual disability (ID),schizophrenia, and bipolar disorder, are caused by the alterationsin early brain development. They affect approximately 4% of the European population and represent a high
socio-economic impact and financial burden. Treatments of NDDs are focused on symptoms since molecular mechanisms underlying NDDs are still unknown. One of the syndromes with a high risk for NDDs
is 22q11.2 Deletion Syndrome (22q11.2DS) caused by microdeletion 22q11.2. 22q11.2 microdeletion is
the most common microdeletion in humans; it is one of the strongest known risk factorsfor development
of psychiatric illness and the highest known genetic risk for schizophrenia (approximately, 25% of patients with 22q11.2DS develop schizophrenia compared to 1% in the general population).
Methods: Genomic and clinical findings in 35 patients with 22q11.2DS were analyzed and peripheral
blood mononuclear cells of patients with 22q11.2DS and healthy controls were reprogrammed.
Results: The majority of patients have 3 Mb deletion and nine of them have inherited 22q11.2 microdeletion from parents. Twenty-one different clinical presentations are revealed in the cohort with developmental delay detected in about 50% of patients. iPSCs were generated from four patients with
22q11.2 microdeletion and five healthy controls.
Conclusion: Cohort of patients with 22q11.2DS isform and iPSCs were generated which enable research
of molecular mechanisms underlying NDDs.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders
EP  - 84
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2138
ER  - 

@conference{
author = "Simeunović, Ivana and Čuturilo, Goran and Kovačević-Grujičić, Nataša and Petter, Olena and Perić, Mina and Kostić, Jovana and Harwood J., Adrian and Stevanović, Milena and Drakulić, Danijela",
year = "2023",
abstract = "Introduction: Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD), intellectual disability (ID),schizophrenia, and bipolar disorder, are caused by the alterationsin early brain development. They affect approximately 4% of the European population and represent a high
socio-economic impact and financial burden. Treatments of NDDs are focused on symptoms since molecular mechanisms underlying NDDs are still unknown. One of the syndromes with a high risk for NDDs
is 22q11.2 Deletion Syndrome (22q11.2DS) caused by microdeletion 22q11.2. 22q11.2 microdeletion is
the most common microdeletion in humans; it is one of the strongest known risk factorsfor development
of psychiatric illness and the highest known genetic risk for schizophrenia (approximately, 25% of patients with 22q11.2DS develop schizophrenia compared to 1% in the general population).
Methods: Genomic and clinical findings in 35 patients with 22q11.2DS were analyzed and peripheral
blood mononuclear cells of patients with 22q11.2DS and healthy controls were reprogrammed.
Results: The majority of patients have 3 Mb deletion and nine of them have inherited 22q11.2 microdeletion from parents. Twenty-one different clinical presentations are revealed in the cohort with developmental delay detected in about 50% of patients. iPSCs were generated from four patients with
22q11.2 microdeletion and five healthy controls.
Conclusion: Cohort of patients with 22q11.2DS isform and iPSCs were generated which enable research
of molecular mechanisms underlying NDDs.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders",
pages = "84-84",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2138"
}

Simeunović, I., Čuturilo, G., Kovačević-Grujičić, N., Petter, O., Perić, M., Kostić, J., Harwood J., A., Stevanović, M.,& Drakulić, D.. (2023). Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 84-84.
https://hdl.handle.net/21.15107/rcub_imagine_2138

Simeunović I, Čuturilo G, Kovačević-Grujičić N, Petter O, Perić M, Kostić J, Harwood J. A, Stevanović M, Drakulić D. Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:84-84.
https://hdl.handle.net/21.15107/rcub_imagine_2138 .

Simeunović, Ivana, Čuturilo, Goran, Kovačević-Grujičić, Nataša, Petter, Olena, Perić, Mina, Kostić, Jovana, Harwood J., Adrian, Stevanović, Milena, Drakulić, Danijela, "Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):84-84,
https://hdl.handle.net/21.15107/rcub_imagine_2138 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Kovačević-Grujičić, Nataša
AU  - Petrović, Isidora
AU  - Drakulić, Danijela
AU  - Milivojević, Milena
AU  - Mojsin, Marija
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/7/6392
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1890
AB  - Glioblastoma (GBM) continues to be the most devastating primary brain malignancy. Despite significant advancements in understanding basic GBM biology and enormous efforts in developing new therapeutic approaches, the prognosis for most GBM patients remains poor with a median survival time of 15 months. Recently, the interplay between the SOX (SRY-related HMG-box) genes and lncRNAs (long non-coding RNAs) has become the focus of GBM research. Both classes of molecules have an aberrant expression in GBM and play essential roles in tumor initiation, progression, therapy resistance, and recurrence. In GBM, SOX and lncRNAs crosstalk through numerous functional axes, some of which are part of the complex transcriptional and epigenetic regulatory mechanisms. This review provides a systematic summary of current literature data on the complex interplay between SOX genes and lncRNAs and represents an effort to underscore the effects of SOX/lncRNA crosstalk on the malignant properties of GBM cells. Furthermore, we highlight the significance of this crosstalk in searching for new biomarkers and therapeutic approaches in GBM treatment.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma
IS  - 7
SP  - 6392
VL  - 24
DO  - 10.3390/ijms24076392
ER  - 

@article{
author = "Stevanović, Milena and Kovačević-Grujičić, Nataša and Petrović, Isidora and Drakulić, Danijela and Milivojević, Milena and Mojsin, Marija",
year = "2023",
abstract = "Glioblastoma (GBM) continues to be the most devastating primary brain malignancy. Despite significant advancements in understanding basic GBM biology and enormous efforts in developing new therapeutic approaches, the prognosis for most GBM patients remains poor with a median survival time of 15 months. Recently, the interplay between the SOX (SRY-related HMG-box) genes and lncRNAs (long non-coding RNAs) has become the focus of GBM research. Both classes of molecules have an aberrant expression in GBM and play essential roles in tumor initiation, progression, therapy resistance, and recurrence. In GBM, SOX and lncRNAs crosstalk through numerous functional axes, some of which are part of the complex transcriptional and epigenetic regulatory mechanisms. This review provides a systematic summary of current literature data on the complex interplay between SOX genes and lncRNAs and represents an effort to underscore the effects of SOX/lncRNA crosstalk on the malignant properties of GBM cells. Furthermore, we highlight the significance of this crosstalk in searching for new biomarkers and therapeutic approaches in GBM treatment.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma",
number = "7",
pages = "6392",
volume = "24",
doi = "10.3390/ijms24076392"
}

Stevanović, M., Kovačević-Grujičić, N., Petrović, I., Drakulić, D., Milivojević, M.,& Mojsin, M.. (2023). Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma. in International Journal of Molecular Sciences, 24(7), 6392.
https://doi.org/10.3390/ijms24076392

Stevanović M, Kovačević-Grujičić N, Petrović I, Drakulić D, Milivojević M, Mojsin M. Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma. in International Journal of Molecular Sciences. 2023;24(7):6392.
doi:10.3390/ijms24076392 .

Stevanović, Milena, Kovačević-Grujičić, Nataša, Petrović, Isidora, Drakulić, Danijela, Milivojević, Milena, Mojsin, Marija, "Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma" in International Journal of Molecular Sciences, 24, no. 7 (2023):6392,
https://doi.org/10.3390/ijms24076392 . .

TY  - CONF
AU  - Kostić, Jovana
AU  - Drakulić, Danijela
AU  - Čuturilo, Goran
AU  - Petter, Olena
AU  - Perić, Mina
AU  - Simeunović, Ivana
AU  - Harwood J., Adrian
AU  - Stevanović, Milena
AU  - Kovačević-Grujičić, Nataša
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2122
AB  - Introduction: Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD), schizophrenia, and intellectual disability, represent important public health challenge in modern societies
with a prevalence of about 10 to 15% of all births and the tendency of increasing worldwide. They are
caused by disruption of early brain development. Treatments of NDDs are focused on symptoms due to
a limited understanding of underlying pathophysiological mechanisms. Individuals with the 22q11.2
Duplication Syndrome (22q11.2dup), caused by heterozygous 22q11.2 microduplication, have an elevated risk of developing NDDs. Literature data revealed that ASD is detected in 14-25% of patients with
22q11.2dup while schizophrenia is less common in these patients than in the general population, suggesting that 22q11.2 duplication might be protective against schizophrenia.
Methods: Genomic and clinical findingsin patients with 22q11.2dup were analyzed and peripheral blood
mononuclear cells of patients with 22q11.2dup were reprogrammed.
Results: We formed a cohort of 8 patients with 22q11.2dup. The majority of patientsin our cohort have
microduplication of approximately 3Mb (80%). Also, the majority of them are familial cases and in 67%
of cases, the 22q11.2 microduplication is inherited from the mother. Congenital heart defects were detected in 25% of our patients, while all tested patients have facial dysmorphism. iPSCs were generated
from three patients with a familial form of 22q11.2dup and their mothers.
Conclusion: A cohort of patients with 22q11.2dup is formed and iPSCs were generated which can be
used as a model system for studying NDDs.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders
EP  - 66
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2122
ER  - 

@conference{
author = "Kostić, Jovana and Drakulić, Danijela and Čuturilo, Goran and Petter, Olena and Perić, Mina and Simeunović, Ivana and Harwood J., Adrian and Stevanović, Milena and Kovačević-Grujičić, Nataša",
year = "2023",
abstract = "Introduction: Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD), schizophrenia, and intellectual disability, represent important public health challenge in modern societies
with a prevalence of about 10 to 15% of all births and the tendency of increasing worldwide. They are
caused by disruption of early brain development. Treatments of NDDs are focused on symptoms due to
a limited understanding of underlying pathophysiological mechanisms. Individuals with the 22q11.2
Duplication Syndrome (22q11.2dup), caused by heterozygous 22q11.2 microduplication, have an elevated risk of developing NDDs. Literature data revealed that ASD is detected in 14-25% of patients with
22q11.2dup while schizophrenia is less common in these patients than in the general population, suggesting that 22q11.2 duplication might be protective against schizophrenia.
Methods: Genomic and clinical findingsin patients with 22q11.2dup were analyzed and peripheral blood
mononuclear cells of patients with 22q11.2dup were reprogrammed.
Results: We formed a cohort of 8 patients with 22q11.2dup. The majority of patientsin our cohort have
microduplication of approximately 3Mb (80%). Also, the majority of them are familial cases and in 67%
of cases, the 22q11.2 microduplication is inherited from the mother. Congenital heart defects were detected in 25% of our patients, while all tested patients have facial dysmorphism. iPSCs were generated
from three patients with a familial form of 22q11.2dup and their mothers.
Conclusion: A cohort of patients with 22q11.2dup is formed and iPSCs were generated which can be
used as a model system for studying NDDs.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2122"
}

Kostić, J., Drakulić, D., Čuturilo, G., Petter, O., Perić, M., Simeunović, I., Harwood J., A., Stevanović, M.,& Kovačević-Grujičić, N.. (2023). Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 66-66.
https://hdl.handle.net/21.15107/rcub_imagine_2122

Kostić J, Drakulić D, Čuturilo G, Petter O, Perić M, Simeunović I, Harwood J. A, Stevanović M, Kovačević-Grujičić N. Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:66-66.
https://hdl.handle.net/21.15107/rcub_imagine_2122 .

Kostić, Jovana, Drakulić, Danijela, Čuturilo, Goran, Petter, Olena, Perić, Mina, Simeunović, Ivana, Harwood J., Adrian, Stevanović, Milena, Kovačević-Grujičić, Nataša, "Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):66-66,
https://hdl.handle.net/21.15107/rcub_imagine_2122 .

TY  - CONF
AU  - Drakulić, Danijela
AU  - Rakonjac, Marijana
AU  - Cuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Kušić-Tišma, Jelena
AU  - Morić, Ivana
AU  - Zukić, Branka
AU  - Stevanović, Milena
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2036
AB  - 22q11.2 deletion syndrome (22q11.2DS is caused by 22q11.2 microdeletion, one of
the strongest known risk factors for development of neurodevelopmental disorders.
About 70% patients with 22q11.2DS have speech and language impairments. In the
literature, there is no data about articulatory characteristics of phonemes of children
with 22q11.2DS, monolingual native speakers of South Slavic languages. Here we, by
applying Global Articulation Test, analyzed articulatory characteristics of phonemes of
children with 22q11.2DS, monolingual native speakers of the Serbian language (group
E1), children with a phenotype resembling 22q11.2DS but without the microdeletion
(group E2), children with non-syndromic congenital heart malformations (since children
with these malformations may exhibit a speech and language impairments) (group
E3) and their peers with typical speech-sound development (group C). Results of PCA
indicated that the groups can be distinguished based on the pronunciation of phonemes,
and that the pronunciation of the phonemes “Č ⟨tʃ⟩”, “Dž ⟨ʤ⟩”, “Š ⟨∫⟩”, “Ž ⟨ʒ⟩”, “R”, and “Lj ⟨ʎ⟩”
contributes the most to the variability between the groups. Results of AHP revealed that
the pronunciation of the phonemes “Č ⟨tʃ⟩”, “Dž ⟨ʤ⟩”, “Š ⟨∫⟩”, “Ž ⟨ʒ⟩”, “R”, and “Lj ⟨ʎ⟩” was
rated the worst in the group E1. In conclusion, obtained results indicate that the presence
of 22q11.2 microdeletion influences articulation skills of carriers.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome
EP  - 91
SP  - 91
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2036
ER  - 

@conference{
author = "Drakulić, Danijela and Rakonjac, Marijana and Cuturilo, Goran and Kovačević-Grujičić, Nataša and Kušić-Tišma, Jelena and Morić, Ivana and Zukić, Branka and Stevanović, Milena",
year = "2023",
abstract = "22q11.2 deletion syndrome (22q11.2DS is caused by 22q11.2 microdeletion, one of
the strongest known risk factors for development of neurodevelopmental disorders.
About 70% patients with 22q11.2DS have speech and language impairments. In the
literature, there is no data about articulatory characteristics of phonemes of children
with 22q11.2DS, monolingual native speakers of South Slavic languages. Here we, by
applying Global Articulation Test, analyzed articulatory characteristics of phonemes of
children with 22q11.2DS, monolingual native speakers of the Serbian language (group
E1), children with a phenotype resembling 22q11.2DS but without the microdeletion
(group E2), children with non-syndromic congenital heart malformations (since children
with these malformations may exhibit a speech and language impairments) (group
E3) and their peers with typical speech-sound development (group C). Results of PCA
indicated that the groups can be distinguished based on the pronunciation of phonemes,
and that the pronunciation of the phonemes “Č ⟨tʃ⟩”, “Dž ⟨ʤ⟩”, “Š ⟨∫⟩”, “Ž ⟨ʒ⟩”, “R”, and “Lj ⟨ʎ⟩”
contributes the most to the variability between the groups. Results of AHP revealed that
the pronunciation of the phonemes “Č ⟨tʃ⟩”, “Dž ⟨ʤ⟩”, “Š ⟨∫⟩”, “Ž ⟨ʒ⟩”, “R”, and “Lj ⟨ʎ⟩” was
rated the worst in the group E1. In conclusion, obtained results indicate that the presence
of 22q11.2 microdeletion influences articulation skills of carriers.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome",
pages = "91-91",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2036"
}

Drakulić, D., Rakonjac, M., Cuturilo, G., Kovačević-Grujičić, N., Kušić-Tišma, J., Morić, I., Zukić, B.,& Stevanović, M.. (2023). Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 91-91.
https://hdl.handle.net/21.15107/rcub_imagine_2036

Drakulić D, Rakonjac M, Cuturilo G, Kovačević-Grujičić N, Kušić-Tišma J, Morić I, Zukić B, Stevanović M. Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome. in 4th Belgrade Bioinformatics Conference. 2023;4:91-91.
https://hdl.handle.net/21.15107/rcub_imagine_2036 .

Drakulić, Danijela, Rakonjac, Marijana, Cuturilo, Goran, Kovačević-Grujičić, Nataša, Kušić-Tišma, Jelena, Morić, Ivana, Zukić, Branka, Stevanović, Milena, "Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome" in 4th Belgrade Bioinformatics Conference, 4 (2023):91-91,
https://hdl.handle.net/21.15107/rcub_imagine_2036 .

TY  - CONF
AU  - Lazić, Adrijana
AU  - Drakulić, Danijela
AU  - Kovačević-Grujičić, Nataša
AU  - Perić, Mina
AU  - Petrakis, Spyros
AU  - Linden, David
AU  - Harwood, Adrian
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2110
AB  - Introduction: Neurodevelopmental disorders (NDDs) are a group of complex and heterogeneous disorders that include autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. However, underlying pathophysiological mechanisms are mostly unknown. In order to get better understanding of the underlying mechanisms and to discover potential therapeutics we have focused our research on 22q11.2 Deletion Syndrome (22q11.2DS), caused by microdeletion of the region q11.2 of chromosome 22 and associated with a high risk for NDDs. Methods: To study molecular mechanisms underlying intrafamilial phenotypic variability, we have identified families with the inherited form of 22q11.2DS with the aim of conducting the following analyses: whole genome sequencing in order to detect additional genetic variation(s) present in the affected child; generation of induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells; analysis of the effects of 22q11.2 microdeletion on neural differentiation including organoids as 3D model system; transcriptome analysis of iPSC-derived neurons and astrocytesto determine differentially expressed gene sets and dysregulated pathways; and testing the metabolic changes and drug responsiveness of neurons and astrocytes by high-throughput cell-based assays. Results: Peripheral blood mononuclear cells of the families with inherited form of 22q11.2DS were reprogrammed and established iPSCs were characterized. Generated iPSCs will be subjected to the further analyses. Conclusion: Currently, most of the treatments of NDDs are symptom-based due to limited understanding of underlying pathophysiological mechanisms. It is expected that patient-derived iPSCs will enable a deeper understanding of unique disease mechanisms and may also provide a significant contribution in preclinical drug development.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - 22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders
EP  - 31
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2110
ER  - 

@conference{
author = "Lazić, Adrijana and Drakulić, Danijela and Kovačević-Grujičić, Nataša and Perić, Mina and Petrakis, Spyros and Linden, David and Harwood, Adrian and Stevanović, Milena",
year = "2023",
abstract = "Introduction: Neurodevelopmental disorders (NDDs) are a group of complex and heterogeneous disorders that include autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. However, underlying pathophysiological mechanisms are mostly unknown. In order to get better understanding of the underlying mechanisms and to discover potential therapeutics we have focused our research on 22q11.2 Deletion Syndrome (22q11.2DS), caused by microdeletion of the region q11.2 of chromosome 22 and associated with a high risk for NDDs. Methods: To study molecular mechanisms underlying intrafamilial phenotypic variability, we have identified families with the inherited form of 22q11.2DS with the aim of conducting the following analyses: whole genome sequencing in order to detect additional genetic variation(s) present in the affected child; generation of induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells; analysis of the effects of 22q11.2 microdeletion on neural differentiation including organoids as 3D model system; transcriptome analysis of iPSC-derived neurons and astrocytesto determine differentially expressed gene sets and dysregulated pathways; and testing the metabolic changes and drug responsiveness of neurons and astrocytes by high-throughput cell-based assays. Results: Peripheral blood mononuclear cells of the families with inherited form of 22q11.2DS were reprogrammed and established iPSCs were characterized. Generated iPSCs will be subjected to the further analyses. Conclusion: Currently, most of the treatments of NDDs are symptom-based due to limited understanding of underlying pathophysiological mechanisms. It is expected that patient-derived iPSCs will enable a deeper understanding of unique disease mechanisms and may also provide a significant contribution in preclinical drug development.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders",
pages = "31-31",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2110"
}

Lazić, A., Drakulić, D., Kovačević-Grujičić, N., Perić, M., Petrakis, S., Linden, D., Harwood, A.,& Stevanović, M.. (2023). 22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 31-31.
https://hdl.handle.net/21.15107/rcub_imagine_2110

Lazić A, Drakulić D, Kovačević-Grujičić N, Perić M, Petrakis S, Linden D, Harwood A, Stevanović M. 22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:31-31.
https://hdl.handle.net/21.15107/rcub_imagine_2110 .

Lazić, Adrijana, Drakulić, Danijela, Kovačević-Grujičić, Nataša, Perić, Mina, Petrakis, Spyros, Linden, David, Harwood, Adrian, Stevanović, Milena, "22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):31-31,
https://hdl.handle.net/21.15107/rcub_imagine_2110 .

TY  - CONF
AU  - Balint, Vanda
AU  - Stanisavljević Ninković, Danijela
AU  - Anastasov, Nataša
AU  - Lazić, Stefan
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
AU  - Krstić, Aleksandra
AU  - Pejić, Jelena
PY  - 2022
UR  - https://doi.org/10.21175/rad.spr.abstr.book.2022.22.1
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1864
AB  - Astrocytes are the main homeostatic cells in the central nervous system (CNS) that provide mechanical,
metabolic, and trophic support to neurons. Disruption of their physiological role or acquisition of
senescence-associated phenotype can contribute to the CNS dysfunction and pathology. However, molecular
mechanisms underlying the complex physiology of astrocytes are explored insufficiently. Recent studies have
shown that miRNAs are involved in the regulation of astrocyte function through different mechanisms.
Although miR-21 has been reported as an astrocytic miRNA with an important role in astrogliosis, no link
between this miRNA and cellular senescence of astrocytes has been identified. To address the role of miR-21
in astrocytes, with special focus on cellular senescence, we used NT2/A (astrocytes derived from NT2/D1
cells). Downregulation of miR-21 expression in both immature and mature NT2/A by the antisense
technology induced the arrest of cell growth and premature cellular senescence, as indicated by senescence
hallmarks such as increased expression of cell cycle inhibitors p21 and p53 and augmented senescenceassociated
β-galactosidase activity. Additionally, in silico analysis predicted many of the genes, previously
shown to be upregulated in astrocytes with the irradiation-induced senescence, as miR-21 targets. Taken
together, our results point to miR-21 as a potential regulator of astrocyte senescence. To the best of our
knowledge, these are the first data showing the link between miR-21 and cellular senescence of astrocytes.
Since senescent astrocytes are associated with different CNS pathologies, development of novel therapeutic
strategies based on miRNA manipulation could prevent senescence and may improve the physiological
outcome.
C3  - RAD International concerence on radiation in various fields of research
T1  - Inhibition of miR-21 promotes cellular senescence in NT2-derived astrocytes
IS  - Spring Edition
SP  - 90
DO  - 10.21175/rad.spr.abstr.book.2022.22.1
ER  - 

@conference{
author = "Balint, Vanda and Stanisavljević Ninković, Danijela and Anastasov, Nataša and Lazić, Stefan and Kovačević-Grujičić, Nataša and Stevanović, Milena and Lazić, Andrijana and Krstić, Aleksandra and Pejić, Jelena",
year = "2022",
abstract = "Astrocytes are the main homeostatic cells in the central nervous system (CNS) that provide mechanical,
metabolic, and trophic support to neurons. Disruption of their physiological role or acquisition of
senescence-associated phenotype can contribute to the CNS dysfunction and pathology. However, molecular
mechanisms underlying the complex physiology of astrocytes are explored insufficiently. Recent studies have
shown that miRNAs are involved in the regulation of astrocyte function through different mechanisms.
Although miR-21 has been reported as an astrocytic miRNA with an important role in astrogliosis, no link
between this miRNA and cellular senescence of astrocytes has been identified. To address the role of miR-21
in astrocytes, with special focus on cellular senescence, we used NT2/A (astrocytes derived from NT2/D1
cells). Downregulation of miR-21 expression in both immature and mature NT2/A by the antisense
technology induced the arrest of cell growth and premature cellular senescence, as indicated by senescence
hallmarks such as increased expression of cell cycle inhibitors p21 and p53 and augmented senescenceassociated
β-galactosidase activity. Additionally, in silico analysis predicted many of the genes, previously
shown to be upregulated in astrocytes with the irradiation-induced senescence, as miR-21 targets. Taken
together, our results point to miR-21 as a potential regulator of astrocyte senescence. To the best of our
knowledge, these are the first data showing the link between miR-21 and cellular senescence of astrocytes.
Since senescent astrocytes are associated with different CNS pathologies, development of novel therapeutic
strategies based on miRNA manipulation could prevent senescence and may improve the physiological
outcome.",
journal = "RAD International concerence on radiation in various fields of research",
title = "Inhibition of miR-21 promotes cellular senescence in NT2-derived astrocytes",
number = "Spring Edition",
pages = "90",
doi = "10.21175/rad.spr.abstr.book.2022.22.1"
}

Balint, V., Stanisavljević Ninković, D., Anastasov, N., Lazić, S., Kovačević-Grujičić, N., Stevanović, M., Lazić, A., Krstić, A.,& Pejić, J.. (2022). Inhibition of miR-21 promotes cellular senescence in NT2-derived astrocytes. in RAD International concerence on radiation in various fields of research(Spring Edition), 90.
https://doi.org/10.21175/rad.spr.abstr.book.2022.22.1

Balint V, Stanisavljević Ninković D, Anastasov N, Lazić S, Kovačević-Grujičić N, Stevanović M, Lazić A, Krstić A, Pejić J. Inhibition of miR-21 promotes cellular senescence in NT2-derived astrocytes. in RAD International concerence on radiation in various fields of research. 2022;(Spring Edition):90.
doi:10.21175/rad.spr.abstr.book.2022.22.1 .

Balint, Vanda, Stanisavljević Ninković, Danijela, Anastasov, Nataša, Lazić, Stefan, Kovačević-Grujičić, Nataša, Stevanović, Milena, Lazić, Andrijana, Krstić, Aleksandra, Pejić, Jelena, "Inhibition of miR-21 promotes cellular senescence in NT2-derived astrocytes" in RAD International concerence on radiation in various fields of research, no. Spring Edition (2022):90,
https://doi.org/10.21175/rad.spr.abstr.book.2022.22.1 . .

TY  - JOUR
AU  - Drakulić, Danijela
AU  - Schwirtlich, Marija
AU  - Petrović, Isidora
AU  - Mojsin, Marija
AU  - Milivojević, Milena
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1519
AB  - Glioblastoma (GBM) is the most common and highly lethal type of brain tumor, with poor survival despite advances in understanding its complexity. After current standard therapeutic treatment, including tumor resection, radiotherapy and concomitant chemotherapy with temozolomide, the median overall survival of patients with this type of tumor is less than 15 months. Thus, there is an urgent need for new insights into GBM molecular characteristics and progress in targeted therapy in order to improve clinical outcomes. The literature data revealed that a number of different signaling pathways are dysregulated in GBM. In this review, we intended to summarize and discuss current literature data and therapeutic modalities focused on targeting dysregulated signaling pathways in GBM. A better understanding of opportunities for targeting signaling pathways that influences malignant behavior of GBM cells might open the way for the development of novel GBM-targeted therapies.
PB  - MDPI, Basel
T2  - Cells
T1  - Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma
IS  - 16
VL  - 11
DO  - 10.3390/cells11162530
ER  - 

@article{
author = "Drakulić, Danijela and Schwirtlich, Marija and Petrović, Isidora and Mojsin, Marija and Milivojević, Milena and Kovačević Grujičić, Nataša and Stevanović, Milena",
year = "2022",
abstract = "Glioblastoma (GBM) is the most common and highly lethal type of brain tumor, with poor survival despite advances in understanding its complexity. After current standard therapeutic treatment, including tumor resection, radiotherapy and concomitant chemotherapy with temozolomide, the median overall survival of patients with this type of tumor is less than 15 months. Thus, there is an urgent need for new insights into GBM molecular characteristics and progress in targeted therapy in order to improve clinical outcomes. The literature data revealed that a number of different signaling pathways are dysregulated in GBM. In this review, we intended to summarize and discuss current literature data and therapeutic modalities focused on targeting dysregulated signaling pathways in GBM. A better understanding of opportunities for targeting signaling pathways that influences malignant behavior of GBM cells might open the way for the development of novel GBM-targeted therapies.",
publisher = "MDPI, Basel",
journal = "Cells",
title = "Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma",
number = "16",
volume = "11",
doi = "10.3390/cells11162530"
}

Drakulić, D., Schwirtlich, M., Petrović, I., Mojsin, M., Milivojević, M., Kovačević Grujičić, N.,& Stevanović, M.. (2022). Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma. in Cells
MDPI, Basel., 11(16).
https://doi.org/10.3390/cells11162530

Drakulić D, Schwirtlich M, Petrović I, Mojsin M, Milivojević M, Kovačević Grujičić N, Stevanović M. Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma. in Cells. 2022;11(16).
doi:10.3390/cells11162530 .

Drakulić, Danijela, Schwirtlich, Marija, Petrović, Isidora, Mojsin, Marija, Milivojević, Milena, Kovačević Grujičić, Nataša, Stevanović, Milena, "Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma" in Cells, 11, no. 16 (2022),
https://doi.org/10.3390/cells11162530 . .

TY  - JOUR
AU  - Holub, Karolina
AU  - Malyarchuk, Boris
AU  - Derenko, Miroslava
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Davidović, Slobodan
AU  - Grzybowski, Tomasz
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1913
AB  - Genetic markers for the prediction of biogeographical ancestry have proved to be effective tools for law enforcement agencies for many years now. In this study, we attempted to assess the potential of insertion-deletion markers (InDel) and microsatellites (STRs) as subsidiary polymorphisms for inference of Slavic population ancestry. For that purpose, we genotyped Slavic-speaking populations samples from Belarus, the Czech Republic, Poland, Serbia, Ukraine and Russia in 46 InDels and 15 STRs by PCR and capillary electrophoresis and analyzed for between-population differentiation with the use of distance-based methods (FST, principal component analysis and multidimensional scaling).Additionally, we studied a sample from a Polish individual of well-documented genealogy whose biogeographic ancestry had previously been inferred by commercial genomic services using autosomal single nucleotide polymorphisms (SNPs), mitochondrial DNA and Y-SNP markers. For comparative purposes, we used genotype data collected in the “forInDel” browser and allele frequencies from previously published papers. The results obtained for InDels and STRs show that the Slavic populations constitute a genetically homogeneous group, with the exception of the Czechs differing clearly from the other tested populations. The analysis of the known Polish sample in the Snipper application proves the usefulness of the InDel markers on the continental level only. Conversely, microsatellites not only improve prediction, but are also informative if considered as an independent set of ancestry markers.
AB  - Markery genetyczne do przewidywania pochodzenia biogeograficznego od wielu lat okazują się skutecznymi narzędziami dla organów ścigania. W tym badaniu podjęliśmy próbę oceny potencjału markerów insercyjno-delecyjnych
(InDel) i mikrosatelitarnych (STR) jako pomocniczych polimorfizmów do wnioskowania o pochodzeniu populacji
słowiańskiej. W tym celu genotypowaliśmy próbki populacji słowiańskojęzycznych z Białorusi, Czech, Polski, Serbii,
Ukrainy i Rosji w w zakresie 46 markerów InDel oraz 15 loci STR za pomocą PCR i elektroforezy kapilarnej oraz
analizowaliśmy pod kątem różnicowania między populacjami za pomocą metod bazujących na dystansach genetycznych (FST, analiza głównych składowych i skalowanie wielowymiarowe). Dodatkowo zbadaliśmy próbkę mężczyzny
z populacji polskiej o dobrze udokumentowanej genealogii, którego pochodzenie biogeograficzne zostało wcześniej
ustalone przez komercyjne usługi genomiczne przy użyciu autosomalnych polimorfizmów pojedynczych nukleotydów (SNP), mitochondrialnego DNA i markerów Y-SNP. Do celów porównawczych wykorzystaliśmy dane genotypowe zebrane w przeglądarce „forInDel” i częstości alleli z wcześniej opublikowanych artykułów. Uzyskane wyniki
dla InDels i STR wskazują, że populacje słowiańskie stanowią grupę genetycznie jednorodną, z wyjątkiem Czechów
wyraźnie różniących się od pozostałych badanych populacji. Analiza znanej polskiej próbki w aplikacji Snipper
dowodzi przydatności markerów InDel jedynie na poziomie kontynentalnym. Z kolei, mikrosatelity nie tylko poprawiają wyniki predykcji, ale są informatywne jako niezależny zestaw markerów pochodzenia biogeograficznego.
PB  - Polish Society of Forensic Medicine and Criminology
T2  - Archives of Forensic Medicine and Criminology
T1  - Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry
T1  - Weryfikacja markerów insercyjno-delecyjnych (InDels)
i mikrosatelitarnych (STR) jako narzędzi pomocniczych
do wnioskowania o pochodzeniu populacji słowiańskiej
EP  - 137
IS  - 3
SP  - 120
VL  - 72
DO  - 10.4467/16891716AMSIK.22.015.17393
ER  - 

@article{
author = "Holub, Karolina and Malyarchuk, Boris and Derenko, Miroslava and Kovačević-Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela and Davidović, Slobodan and Grzybowski, Tomasz",
year = "2022",
abstract = "Genetic markers for the prediction of biogeographical ancestry have proved to be effective tools for law enforcement agencies for many years now. In this study, we attempted to assess the potential of insertion-deletion markers (InDel) and microsatellites (STRs) as subsidiary polymorphisms for inference of Slavic population ancestry. For that purpose, we genotyped Slavic-speaking populations samples from Belarus, the Czech Republic, Poland, Serbia, Ukraine and Russia in 46 InDels and 15 STRs by PCR and capillary electrophoresis and analyzed for between-population differentiation with the use of distance-based methods (FST, principal component analysis and multidimensional scaling).Additionally, we studied a sample from a Polish individual of well-documented genealogy whose biogeographic ancestry had previously been inferred by commercial genomic services using autosomal single nucleotide polymorphisms (SNPs), mitochondrial DNA and Y-SNP markers. For comparative purposes, we used genotype data collected in the “forInDel” browser and allele frequencies from previously published papers. The results obtained for InDels and STRs show that the Slavic populations constitute a genetically homogeneous group, with the exception of the Czechs differing clearly from the other tested populations. The analysis of the known Polish sample in the Snipper application proves the usefulness of the InDel markers on the continental level only. Conversely, microsatellites not only improve prediction, but are also informative if considered as an independent set of ancestry markers., Markery genetyczne do przewidywania pochodzenia biogeograficznego od wielu lat okazują się skutecznymi narzędziami dla organów ścigania. W tym badaniu podjęliśmy próbę oceny potencjału markerów insercyjno-delecyjnych
(InDel) i mikrosatelitarnych (STR) jako pomocniczych polimorfizmów do wnioskowania o pochodzeniu populacji
słowiańskiej. W tym celu genotypowaliśmy próbki populacji słowiańskojęzycznych z Białorusi, Czech, Polski, Serbii,
Ukrainy i Rosji w w zakresie 46 markerów InDel oraz 15 loci STR za pomocą PCR i elektroforezy kapilarnej oraz
analizowaliśmy pod kątem różnicowania między populacjami za pomocą metod bazujących na dystansach genetycznych (FST, analiza głównych składowych i skalowanie wielowymiarowe). Dodatkowo zbadaliśmy próbkę mężczyzny
z populacji polskiej o dobrze udokumentowanej genealogii, którego pochodzenie biogeograficzne zostało wcześniej
ustalone przez komercyjne usługi genomiczne przy użyciu autosomalnych polimorfizmów pojedynczych nukleotydów (SNP), mitochondrialnego DNA i markerów Y-SNP. Do celów porównawczych wykorzystaliśmy dane genotypowe zebrane w przeglądarce „forInDel” i częstości alleli z wcześniej opublikowanych artykułów. Uzyskane wyniki
dla InDels i STR wskazują, że populacje słowiańskie stanowią grupę genetycznie jednorodną, z wyjątkiem Czechów
wyraźnie różniących się od pozostałych badanych populacji. Analiza znanej polskiej próbki w aplikacji Snipper
dowodzi przydatności markerów InDel jedynie na poziomie kontynentalnym. Z kolei, mikrosatelity nie tylko poprawiają wyniki predykcji, ale są informatywne jako niezależny zestaw markerów pochodzenia biogeograficznego.",
publisher = "Polish Society of Forensic Medicine and Criminology",
journal = "Archives of Forensic Medicine and Criminology",
title = "Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry, Weryfikacja markerów insercyjno-delecyjnych (InDels)
i mikrosatelitarnych (STR) jako narzędzi pomocniczych
do wnioskowania o pochodzeniu populacji słowiańskiej",
pages = "137-120",
number = "3",
volume = "72",
doi = "10.4467/16891716AMSIK.22.015.17393"
}

Holub, K., Malyarchuk, B., Derenko, M., Kovačević-Grujičić, N., Stevanović, M., Drakulić, D., Davidović, S.,& Grzybowski, T.. (2022). Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry. in Archives of Forensic Medicine and Criminology
Polish Society of Forensic Medicine and Criminology., 72(3), 120-137.
https://doi.org/10.4467/16891716AMSIK.22.015.17393

Holub K, Malyarchuk B, Derenko M, Kovačević-Grujičić N, Stevanović M, Drakulić D, Davidović S, Grzybowski T. Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry. in Archives of Forensic Medicine and Criminology. 2022;72(3):120-137.
doi:10.4467/16891716AMSIK.22.015.17393 .

Holub, Karolina, Malyarchuk, Boris, Derenko, Miroslava, Kovačević-Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, Davidović, Slobodan, Grzybowski, Tomasz, "Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry" in Archives of Forensic Medicine and Criminology, 72, no. 3 (2022):120-137,
https://doi.org/10.4467/16891716AMSIK.22.015.17393 . .

TY  - CONF
AU  - Lazić, Stefan
AU  - Dužanić, Filip
AU  - Stanisavljević Ninković, Danijela
AU  - Drakulić, Danijela
AU  - Mojsin, Marija
AU  - Milojević, Milena
AU  - Balinat, Vanda
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša 
AU  - Schwirtlich, Marija
AU  - Stevanović, Milena
PY  - 2022
UR  - https://doi.org/10.21175/rad.spr.abstr.book.2022.22.2
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1865
AB  - The family of SOX genes encodes proteins that display properties of both classical transcription factors and architectural components of chromatin. During development of nervous system, as well as adult neurogenesis, SOX transcription factors govern diverse cellular processes such as maintaining the multipotency of neural stem cells, cell proliferation, cell fate decision, migration as well as terminal differentiation of neurons. Despite their well-known function in development and brain homeostasis, the expression and role of these genes in pathology- induced neural stem cell plasticity is poorly understood. Reduction in oxygen supply or ischemia are involved in various pathological conditions, such as stroke, traumatic brain injury and cardiac arrest, which promotes neurogenesis, angiogenesis, cell proliferation and other cell mechanisms for survival under the stress. The aim of the present study was to analyze the expression of SOX genes during in vitro neurogenesis following chemical hypoxia. Neuronal differentiation of human pluripotent embryonal carcinoma stem cell line NT2/D1 was used as an in vitro model system for studying the process of human neurogenesis. Depending on different concentration, RA directed the differentiation of NT2/D1 cells into neurons with a different phenotype. The effect of stress caused by hypoxia on the properties of pluripotent cells as well as the induction of neural differentiation was monitored in vitro by culturing NT2/D1 cells in the presence of cobalt chloride, a chemical inducer of hypoxia. The results of the analysis showed that the effect of hypoxia on the expression of SOX2 and OCT4 proteins involved in maintaining the pluripotency of cells depends on the duration of action of cobalt chloride. After short-term exposure of the cells, an increase in the levels of expression of SOX2 and OCT4 proteins was detected, while long-term treatment of the cells led to a decrease in the expression of these proteins. Furthermore, results showed that depending of duration of cobalt chloride treatments, the level of expression of miR-21 in undifferentiated NT2/D1 cells significantly changed. In addition, long-term pretreatment of pluripotent cells with cobalt chloride resulted in increased expression levels of SOX2, SOX3 and GAD67 proteins in neural progenitors induced for 7 days in the presence of, either low or high concentration of retinoic acid, indicating that hypoxia causes increased efficiency of NT2/D1 cell neural differentiation. Damage of brain tissue caused by reduction of oxygen and/or blood flow to the tissue is the leading cause of death worldwide and the leading cause of disability in humans. Our results contributes to the research focused on discovering the roles of SOX TFs and their gene targets in ischemia related pathologies, making them promising biomarkers and potential targets for future diagnostic and therapeutic strategies.
C3  - RAD International concerence on radiation in various fields of research
T1  - Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells
IS  - Spring Edition
SP  - 91
DO  - 10.21175/rad.spr.abstr.book.2022.22.2
ER  - 

@conference{
author = "Lazić, Stefan and Dužanić, Filip and Stanisavljević Ninković, Danijela and Drakulić, Danijela and Mojsin, Marija and Milojević, Milena and Balinat, Vanda and Petrović, Isidora and Kovačević Grujičić, Nataša  and Schwirtlich, Marija and Stevanović, Milena",
year = "2022",
abstract = "The family of SOX genes encodes proteins that display properties of both classical transcription factors and architectural components of chromatin. During development of nervous system, as well as adult neurogenesis, SOX transcription factors govern diverse cellular processes such as maintaining the multipotency of neural stem cells, cell proliferation, cell fate decision, migration as well as terminal differentiation of neurons. Despite their well-known function in development and brain homeostasis, the expression and role of these genes in pathology- induced neural stem cell plasticity is poorly understood. Reduction in oxygen supply or ischemia are involved in various pathological conditions, such as stroke, traumatic brain injury and cardiac arrest, which promotes neurogenesis, angiogenesis, cell proliferation and other cell mechanisms for survival under the stress. The aim of the present study was to analyze the expression of SOX genes during in vitro neurogenesis following chemical hypoxia. Neuronal differentiation of human pluripotent embryonal carcinoma stem cell line NT2/D1 was used as an in vitro model system for studying the process of human neurogenesis. Depending on different concentration, RA directed the differentiation of NT2/D1 cells into neurons with a different phenotype. The effect of stress caused by hypoxia on the properties of pluripotent cells as well as the induction of neural differentiation was monitored in vitro by culturing NT2/D1 cells in the presence of cobalt chloride, a chemical inducer of hypoxia. The results of the analysis showed that the effect of hypoxia on the expression of SOX2 and OCT4 proteins involved in maintaining the pluripotency of cells depends on the duration of action of cobalt chloride. After short-term exposure of the cells, an increase in the levels of expression of SOX2 and OCT4 proteins was detected, while long-term treatment of the cells led to a decrease in the expression of these proteins. Furthermore, results showed that depending of duration of cobalt chloride treatments, the level of expression of miR-21 in undifferentiated NT2/D1 cells significantly changed. In addition, long-term pretreatment of pluripotent cells with cobalt chloride resulted in increased expression levels of SOX2, SOX3 and GAD67 proteins in neural progenitors induced for 7 days in the presence of, either low or high concentration of retinoic acid, indicating that hypoxia causes increased efficiency of NT2/D1 cell neural differentiation. Damage of brain tissue caused by reduction of oxygen and/or blood flow to the tissue is the leading cause of death worldwide and the leading cause of disability in humans. Our results contributes to the research focused on discovering the roles of SOX TFs and their gene targets in ischemia related pathologies, making them promising biomarkers and potential targets for future diagnostic and therapeutic strategies.",
journal = "RAD International concerence on radiation in various fields of research",
title = "Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells",
number = "Spring Edition",
pages = "91",
doi = "10.21175/rad.spr.abstr.book.2022.22.2"
}

Lazić, S., Dužanić, F., Stanisavljević Ninković, D., Drakulić, D., Mojsin, M., Milojević, M., Balinat, V., Petrović, I., Kovačević Grujičić, N., Schwirtlich, M.,& Stevanović, M.. (2022). Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells. in RAD International concerence on radiation in various fields of research(Spring Edition), 91.
https://doi.org/10.21175/rad.spr.abstr.book.2022.22.2

Lazić S, Dužanić F, Stanisavljević Ninković D, Drakulić D, Mojsin M, Milojević M, Balinat V, Petrović I, Kovačević Grujičić N, Schwirtlich M, Stevanović M. Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells. in RAD International concerence on radiation in various fields of research. 2022;(Spring Edition):91.
doi:10.21175/rad.spr.abstr.book.2022.22.2 .

Lazić, Stefan, Dužanić, Filip, Stanisavljević Ninković, Danijela, Drakulić, Danijela, Mojsin, Marija, Milojević, Milena, Balinat, Vanda, Petrović, Isidora, Kovačević Grujičić, Nataša , Schwirtlich, Marija, Stevanović, Milena, "Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells" in RAD International concerence on radiation in various fields of research, no. Spring Edition (2022):91,
https://doi.org/10.21175/rad.spr.abstr.book.2022.22.2 . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Milivojević, Milena
AU  - Arsenijević, Ana
AU  - Lazić, Andrijana
AU  - Kovačević Grujičić, Nataša
AU  - Schwirtlich, Marija
AU  - Popović, Jelena
AU  - Stevanović, Milena
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1505
AB  - Genetic and molecular heterogeneity, together with intrinsic and acquired resistance to therapy, represent the major obstacles to the successful treatment of different types of breast carcinoma. Increasing evidence demonstrates that SOX transcription factors in breast carcinomas could act both as oncogenes and tumor suppressors and have been associated with tumor stage and grade, poor prognosis, and therapy resistance. Both SOX2 and SOX18 overexpression has been correlated with poor prognosis in breast carcinomas, and these genes are recognized as potential antitumor targets. Our aim was to evaluate the effect of retinoic acid (RA), a well-known cyto-differentiating agent, on breast carcinoma cells in vitro and to investigate the potential of RA treatment to modify the expression of SOX2 and SOX18 genes. By applying various experimental approaches, we evaluated the effect of RA on basic cellular processes in SK-BR-3 and MCF7 breast carcinoma cell lines. We have shown that RA inhibits cell growth, reduces the number of Ki-67 positive cells, and causes cell-cycle arrest. RA effect was more prominent in SK-BR-3 cell line that lacks SOX2 expression, including a higher decrease in cell viability, reduction in colony formation, and significant remodeling of cellular structure. We have shown that RA treatment led to the downregulation of SOX2 expression in MCF7 cells and to the reduction of SOX18 expression in both cell lines. By functional analysis, we showed that the anti-proliferative effect of RA in both cell lines was not based on the activity of stemness marker SOX2, pointing to a SOX2-independent mechanism of action. The ability of RA to reduce SOX2/SOX18 expression raises the possibility that these genes can be used as biomarkers to distinguish RA-responders from non-responders. Together, our study shows that the response of breast carcinoma cell lines to RA treatment may vary, highlighting that the development of RA-based therapy should consider differences in breast carcinoma subtypes.
PB  - Tech Science Press, Henderson
T2  - Biocell
T1  - Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells
EP  - 1367
IS  - 5
SP  - 1355
VL  - 45
DO  - 10.32604/biocell.2021.015817
ER  - 

@article{
author = "Petrović, Isidora and Milivojević, Milena and Arsenijević, Ana and Lazić, Andrijana and Kovačević Grujičić, Nataša and Schwirtlich, Marija and Popović, Jelena and Stevanović, Milena",
year = "2021",
abstract = "Genetic and molecular heterogeneity, together with intrinsic and acquired resistance to therapy, represent the major obstacles to the successful treatment of different types of breast carcinoma. Increasing evidence demonstrates that SOX transcription factors in breast carcinomas could act both as oncogenes and tumor suppressors and have been associated with tumor stage and grade, poor prognosis, and therapy resistance. Both SOX2 and SOX18 overexpression has been correlated with poor prognosis in breast carcinomas, and these genes are recognized as potential antitumor targets. Our aim was to evaluate the effect of retinoic acid (RA), a well-known cyto-differentiating agent, on breast carcinoma cells in vitro and to investigate the potential of RA treatment to modify the expression of SOX2 and SOX18 genes. By applying various experimental approaches, we evaluated the effect of RA on basic cellular processes in SK-BR-3 and MCF7 breast carcinoma cell lines. We have shown that RA inhibits cell growth, reduces the number of Ki-67 positive cells, and causes cell-cycle arrest. RA effect was more prominent in SK-BR-3 cell line that lacks SOX2 expression, including a higher decrease in cell viability, reduction in colony formation, and significant remodeling of cellular structure. We have shown that RA treatment led to the downregulation of SOX2 expression in MCF7 cells and to the reduction of SOX18 expression in both cell lines. By functional analysis, we showed that the anti-proliferative effect of RA in both cell lines was not based on the activity of stemness marker SOX2, pointing to a SOX2-independent mechanism of action. The ability of RA to reduce SOX2/SOX18 expression raises the possibility that these genes can be used as biomarkers to distinguish RA-responders from non-responders. Together, our study shows that the response of breast carcinoma cell lines to RA treatment may vary, highlighting that the development of RA-based therapy should consider differences in breast carcinoma subtypes.",
publisher = "Tech Science Press, Henderson",
journal = "Biocell",
title = "Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells",
pages = "1367-1355",
number = "5",
volume = "45",
doi = "10.32604/biocell.2021.015817"
}

Petrović, I., Milivojević, M., Arsenijević, A., Lazić, A., Kovačević Grujičić, N., Schwirtlich, M., Popović, J.,& Stevanović, M.. (2021). Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells. in Biocell
Tech Science Press, Henderson., 45(5), 1355-1367.
https://doi.org/10.32604/biocell.2021.015817

Petrović I, Milivojević M, Arsenijević A, Lazić A, Kovačević Grujičić N, Schwirtlich M, Popović J, Stevanović M. Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells. in Biocell. 2021;45(5):1355-1367.
doi:10.32604/biocell.2021.015817 .

Petrović, Isidora, Milivojević, Milena, Arsenijević, Ana, Lazić, Andrijana, Kovačević Grujičić, Nataša, Schwirtlich, Marija, Popović, Jelena, Stevanović, Milena, "Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells" in Biocell, 45, no. 5 (2021):1355-1367,
https://doi.org/10.32604/biocell.2021.015817 . .

TY  - JOUR
AU  - Balint, Vanda
AU  - Stanisavljević Ninković, Danijela
AU  - Anastasov, Nataša
AU  - Lazić, Stefan
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1472
AB  - Astrocytes are the main homeostatic cells in the central nervous system (CNS) that provide mechanical, metabolic, and trophic support to neurons. Disruption of their physiological role or acquisition of senescence-associated phenotype can contribute to the CNS dysfunction and pathology. However, molecular mechanisms underlying the complex physiology of astrocytes are explored insufficiently. Recent studies have shown that miRNAs are involved in the regulation of astrocyte function through different mechanisms. Although miR-21 has been reported as an astrocytic miRNA with an important role in astrogliosis, no link between this miRNA and cellular senescence of astrocytes has been identified. To address the role of miR-21 in astrocytes, with special focus on cellular senescence, we used NT2/A (astrocytes derived from NT2/D1 cells). Downregulation of miR-21 expression in both immature and mature NT2/A by the antisense technology induced the arrest of cell growth and premature cellular senescence, as indicated by senescence hallmarks such as increased expression of cell cycle inhibitors p21 and p53 and augmented senescence-associated beta-galactosidase activity. Additionally, in silico analysis predicted many of the genes, previously shown to be upregulated in astrocytes with the irradiation-induced senescence, as miR-21 targets. Taken together, our results point to miR-21 as a potential regulator of astrocyte senescence. To the best of our knowledge, these are the first data showing the link between miR-21 and cellular senescence of astrocytes. Since senescent astrocytes are associated with different CNS pathologies, development of novel therapeutic strategies based on miRNA manipulation could prevent senescence and may improve the physiological outcome.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Biochemistry-Moscow
T1  - Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes
EP  - 1445
IS  - 11
SP  - 1434
VL  - 86
DO  - 10.1134/S0006297921110079
ER  - 

@article{
author = "Balint, Vanda and Stanisavljević Ninković, Danijela and Anastasov, Nataša and Lazić, Stefan and Kovačević Grujičić, Nataša and Stevanović, Milena and Lazić, Andrijana",
year = "2021",
abstract = "Astrocytes are the main homeostatic cells in the central nervous system (CNS) that provide mechanical, metabolic, and trophic support to neurons. Disruption of their physiological role or acquisition of senescence-associated phenotype can contribute to the CNS dysfunction and pathology. However, molecular mechanisms underlying the complex physiology of astrocytes are explored insufficiently. Recent studies have shown that miRNAs are involved in the regulation of astrocyte function through different mechanisms. Although miR-21 has been reported as an astrocytic miRNA with an important role in astrogliosis, no link between this miRNA and cellular senescence of astrocytes has been identified. To address the role of miR-21 in astrocytes, with special focus on cellular senescence, we used NT2/A (astrocytes derived from NT2/D1 cells). Downregulation of miR-21 expression in both immature and mature NT2/A by the antisense technology induced the arrest of cell growth and premature cellular senescence, as indicated by senescence hallmarks such as increased expression of cell cycle inhibitors p21 and p53 and augmented senescence-associated beta-galactosidase activity. Additionally, in silico analysis predicted many of the genes, previously shown to be upregulated in astrocytes with the irradiation-induced senescence, as miR-21 targets. Taken together, our results point to miR-21 as a potential regulator of astrocyte senescence. To the best of our knowledge, these are the first data showing the link between miR-21 and cellular senescence of astrocytes. Since senescent astrocytes are associated with different CNS pathologies, development of novel therapeutic strategies based on miRNA manipulation could prevent senescence and may improve the physiological outcome.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Biochemistry-Moscow",
title = "Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes",
pages = "1445-1434",
number = "11",
volume = "86",
doi = "10.1134/S0006297921110079"
}

Balint, V., Stanisavljević Ninković, D., Anastasov, N., Lazić, S., Kovačević Grujičić, N., Stevanović, M.,& Lazić, A.. (2021). Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes. in Biochemistry-Moscow
Maik Nauka/Interperiodica/Springer, New York., 86(11), 1434-1445.
https://doi.org/10.1134/S0006297921110079

Balint V, Stanisavljević Ninković D, Anastasov N, Lazić S, Kovačević Grujičić N, Stevanović M, Lazić A. Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes. in Biochemistry-Moscow. 2021;86(11):1434-1445.
doi:10.1134/S0006297921110079 .

Balint, Vanda, Stanisavljević Ninković, Danijela, Anastasov, Nataša, Lazić, Stefan, Kovačević Grujičić, Nataša, Stevanović, Milena, Lazić, Andrijana, "Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes" in Biochemistry-Moscow, 86, no. 11 (2021):1434-1445,
https://doi.org/10.1134/S0006297921110079 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Kovačević Grujičić, Nataša
AU  - Mojsin, Marija
AU  - Milivojević, Milena
AU  - Drakulić, Danijela
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1468
AB  - Glioblastoma (GBM) is the most common, most aggressive and deadliest brain tumor. Recently, remarkable progress has been made towards understanding the cellular and molecular biology of gliomas. GBM tumor initiation, progression and relapse as well as resistance to treatments are associated with glioma stem cells (GSCs). GSCs exhibit a high proliferation rate and self-renewal capacity and the ability to differentiate into diverse cell types, generating a range of distinct cell types within the tumor, leading to cellular heterogeneity. GBM tumors may contain different subsets of GSCs, and some of them may adopt a quiescent state that protects them against chemotherapy and radiotherapy. GSCs enriched in recurrent gliomas acquire more aggressive and therapy-resistant properties, making them more malignant, able to rapidly spread. The impact of SOX transcription factors (TFs) on brain tumors has been extensively studied in the last decade. Almost all SOX genes are expressed in GBM, and their expression levels are associated with patient prognosis and survival. Numerous SOX TFs are involved in the maintenance of the stemness of GSCs or play a role in the initiation of GSC differentiation. The fine-tuning of SOX gene expression levels controls the balance between cell stemness and differentiation. Therefore, innovative therapies targeting SOX TFs are emerging as promising tools for combatting GBM. Combatting GBM has been a demanding and challenging goal for decades. The current therapeutic strategies have not yet provided a cure for GBM and have only resulted in a slight improvement in patient survival. Novel approaches will require the fine adjustment of multimodal therapeutic strategies that simultaneously target numerous hallmarks of cancer cells to win the battle against GBM.
PB  - Baishideng Publishing Group Inc, Pleasanton
T2  - World Journal of Stem Cells
T1  - SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation
EP  - 1445
IS  - 10
SP  - 1417
VL  - 13
DO  - 10.4252/wjsc.v13.i10.1417
ER  - 

@article{
author = "Stevanović, Milena and Kovačević Grujičić, Nataša and Mojsin, Marija and Milivojević, Milena and Drakulić, Danijela",
year = "2021",
abstract = "Glioblastoma (GBM) is the most common, most aggressive and deadliest brain tumor. Recently, remarkable progress has been made towards understanding the cellular and molecular biology of gliomas. GBM tumor initiation, progression and relapse as well as resistance to treatments are associated with glioma stem cells (GSCs). GSCs exhibit a high proliferation rate and self-renewal capacity and the ability to differentiate into diverse cell types, generating a range of distinct cell types within the tumor, leading to cellular heterogeneity. GBM tumors may contain different subsets of GSCs, and some of them may adopt a quiescent state that protects them against chemotherapy and radiotherapy. GSCs enriched in recurrent gliomas acquire more aggressive and therapy-resistant properties, making them more malignant, able to rapidly spread. The impact of SOX transcription factors (TFs) on brain tumors has been extensively studied in the last decade. Almost all SOX genes are expressed in GBM, and their expression levels are associated with patient prognosis and survival. Numerous SOX TFs are involved in the maintenance of the stemness of GSCs or play a role in the initiation of GSC differentiation. The fine-tuning of SOX gene expression levels controls the balance between cell stemness and differentiation. Therefore, innovative therapies targeting SOX TFs are emerging as promising tools for combatting GBM. Combatting GBM has been a demanding and challenging goal for decades. The current therapeutic strategies have not yet provided a cure for GBM and have only resulted in a slight improvement in patient survival. Novel approaches will require the fine adjustment of multimodal therapeutic strategies that simultaneously target numerous hallmarks of cancer cells to win the battle against GBM.",
publisher = "Baishideng Publishing Group Inc, Pleasanton",
journal = "World Journal of Stem Cells",
title = "SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation",
pages = "1445-1417",
number = "10",
volume = "13",
doi = "10.4252/wjsc.v13.i10.1417"
}

Stevanović, M., Kovačević Grujičić, N., Mojsin, M., Milivojević, M.,& Drakulić, D.. (2021). SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation. in World Journal of Stem Cells
Baishideng Publishing Group Inc, Pleasanton., 13(10), 1417-1445.
https://doi.org/10.4252/wjsc.v13.i10.1417

Stevanović M, Kovačević Grujičić N, Mojsin M, Milivojević M, Drakulić D. SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation. in World Journal of Stem Cells. 2021;13(10):1417-1445.
doi:10.4252/wjsc.v13.i10.1417 .

Stevanović, Milena, Kovačević Grujičić, Nataša, Mojsin, Marija, Milivojević, Milena, Drakulić, Danijela, "SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation" in World Journal of Stem Cells, 13, no. 10 (2021):1417-1445,
https://doi.org/10.4252/wjsc.v13.i10.1417 . .

TY  - JOUR
AU  - Davidović, Slobodan
AU  - Malyarchuk, Boris
AU  - Grzybowski, Tomasz
AU  - Aleksić, Jelena M.
AU  - Derenko, Miroslava
AU  - Litvinov, Andrey
AU  - Rogalla-Ladniak, Urszula
AU  - Stevanović, Milena
AU  - Kovačević Grujičić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1324
AB  - Mitochondrial genome (mtDNA) is a valuable resource in resolving various human forensic casework. The usage of variability of complete mtDNA genomes increases their discriminatory power to the maximum and enables ultimate resolution of distinct maternal lineages. However, their wider employment in forensic casework is nowadays limited by the lack of appropriate reference database. In order to fill in the gap in the reference data, which, considering Slavic-speaking populations, currently comprises only mitogenomes of East and West Slavs, we present mitogenome data for 226 Serbians, representatives of South Slavs from the Balkan Peninsula. We found 143 (sub)haplogroups among which West Eurasian ones were dominant. The percentage of unique haplotypes was 85%, and the random match probability was as low as 0.53%. We support previous findings on both high levels of genetic diversity in the Serbian population and patterns of genetic differentiation among this and ten studied European populations. However, our high-resolution data supported more pronounced genetic differentiation among Serbians and two Slavic populations (Russians and Poles) as well as expansion of the Serbian population after the Last Glacial Maximum and during the Migration period (fourth to ninth century A.D.), as inferred from the Bayesian skyline analysis. Phylogenetic analysis of haplotypes found in Serbians contributed towards the improvement of the worldwide mtDNA phylogeny, which is essential for the interpretation of the mtDNA casework.
PB  - New York : Springer
T2  - International Journal of Legal Medicine
T1  - Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases
EP  - 1590
IS  - 5
SP  - 1581
VL  - 134
DO  - 10.1007/s00414-020-02324-x
ER  - 

@article{
author = "Davidović, Slobodan and Malyarchuk, Boris and Grzybowski, Tomasz and Aleksić, Jelena M. and Derenko, Miroslava and Litvinov, Andrey and Rogalla-Ladniak, Urszula and Stevanović, Milena and Kovačević Grujičić, Nataša",
year = "2020",
abstract = "Mitochondrial genome (mtDNA) is a valuable resource in resolving various human forensic casework. The usage of variability of complete mtDNA genomes increases their discriminatory power to the maximum and enables ultimate resolution of distinct maternal lineages. However, their wider employment in forensic casework is nowadays limited by the lack of appropriate reference database. In order to fill in the gap in the reference data, which, considering Slavic-speaking populations, currently comprises only mitogenomes of East and West Slavs, we present mitogenome data for 226 Serbians, representatives of South Slavs from the Balkan Peninsula. We found 143 (sub)haplogroups among which West Eurasian ones were dominant. The percentage of unique haplotypes was 85%, and the random match probability was as low as 0.53%. We support previous findings on both high levels of genetic diversity in the Serbian population and patterns of genetic differentiation among this and ten studied European populations. However, our high-resolution data supported more pronounced genetic differentiation among Serbians and two Slavic populations (Russians and Poles) as well as expansion of the Serbian population after the Last Glacial Maximum and during the Migration period (fourth to ninth century A.D.), as inferred from the Bayesian skyline analysis. Phylogenetic analysis of haplotypes found in Serbians contributed towards the improvement of the worldwide mtDNA phylogeny, which is essential for the interpretation of the mtDNA casework.",
publisher = "New York : Springer",
journal = "International Journal of Legal Medicine",
title = "Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases",
pages = "1590-1581",
number = "5",
volume = "134",
doi = "10.1007/s00414-020-02324-x"
}

Davidović, S., Malyarchuk, B., Grzybowski, T., Aleksić, J. M., Derenko, M., Litvinov, A., Rogalla-Ladniak, U., Stevanović, M.,& Kovačević Grujičić, N.. (2020). Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases. in International Journal of Legal Medicine
New York : Springer., 134(5), 1581-1590.
https://doi.org/10.1007/s00414-020-02324-x

Davidović S, Malyarchuk B, Grzybowski T, Aleksić JM, Derenko M, Litvinov A, Rogalla-Ladniak U, Stevanović M, Kovačević Grujičić N. Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases. in International Journal of Legal Medicine. 2020;134(5):1581-1590.
doi:10.1007/s00414-020-02324-x .

Davidović, Slobodan, Malyarchuk, Boris, Grzybowski, Tomasz, Aleksić, Jelena M., Derenko, Miroslava, Litvinov, Andrey, Rogalla-Ladniak, Urszula, Stevanović, Milena, Kovačević Grujičić, Nataša, "Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases" in International Journal of Legal Medicine, 134, no. 5 (2020):1581-1590,
https://doi.org/10.1007/s00414-020-02324-x . .

TY  - JOUR
AU  - Tošić, Nataša
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Davidović, Slobodan
AU  - Virijević, Marijana
AU  - Suvajdžić-Vuković, Nada
AU  - Pavlović, Sonja
AU  - Stevanović, Milena
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1196
AB  - Aberrant expression of different SOX (SRY-related high mobility group (HMG) box) genes has been observed in number of tumors but, little is known about their expression patterns in hematological malignancies, especially in acute myeloid leukemia (AML). In this study we investigated SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in 50 de novo adult AML patients and correlated our findings with known clinical and molecular prognostic markers of the disease. We have found that these genes are overexpressed in 10-22% of patients and preliminary findings suggest that high expression level of these genes may have prognostic significance in AML patients. This is the first study focused on examining the expression level of SOX2, SOX3, SOX11, SOX14 and SOX18 genes in AML patients. Although this is a relatively limited study, initial findings indicate the need for further investigation of these genes, their potential roles in leukemia pathogenesis as well as prognosis in AML patients.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Leukemia Research
T1  - Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia
EP  - 38
SP  - 32
VL  - 67
DO  - 10.1016/j.leukres.2018.02.001
ER  - 

@article{
author = "Tošić, Nataša and Petrović, Isidora and Kovačević Grujičić, Nataša and Davidović, Slobodan and Virijević, Marijana and Suvajdžić-Vuković, Nada and Pavlović, Sonja and Stevanović, Milena",
year = "2018",
abstract = "Aberrant expression of different SOX (SRY-related high mobility group (HMG) box) genes has been observed in number of tumors but, little is known about their expression patterns in hematological malignancies, especially in acute myeloid leukemia (AML). In this study we investigated SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in 50 de novo adult AML patients and correlated our findings with known clinical and molecular prognostic markers of the disease. We have found that these genes are overexpressed in 10-22% of patients and preliminary findings suggest that high expression level of these genes may have prognostic significance in AML patients. This is the first study focused on examining the expression level of SOX2, SOX3, SOX11, SOX14 and SOX18 genes in AML patients. Although this is a relatively limited study, initial findings indicate the need for further investigation of these genes, their potential roles in leukemia pathogenesis as well as prognosis in AML patients.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Leukemia Research",
title = "Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia",
pages = "38-32",
volume = "67",
doi = "10.1016/j.leukres.2018.02.001"
}

Tošić, N., Petrović, I., Kovačević Grujičić, N., Davidović, S., Virijević, M., Suvajdžić-Vuković, N., Pavlović, S.,& Stevanović, M.. (2018). Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia. in Leukemia Research
Pergamon-Elsevier Science Ltd, Oxford., 67, 32-38.
https://doi.org/10.1016/j.leukres.2018.02.001

Tošić N, Petrović I, Kovačević Grujičić N, Davidović S, Virijević M, Suvajdžić-Vuković N, Pavlović S, Stevanović M. Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia. in Leukemia Research. 2018;67:32-38.
doi:10.1016/j.leukres.2018.02.001 .

Tošić, Nataša, Petrović, Isidora, Kovačević Grujičić, Nataša, Davidović, Slobodan, Virijević, Marijana, Suvajdžić-Vuković, Nada, Pavlović, Sonja, Stevanović, Milena, "Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia" in Leukemia Research, 67 (2018):32-38,
https://doi.org/10.1016/j.leukres.2018.02.001 . .

TY  - CONF
AU  - Paunesku, Tatjana
AU  - Popović, Jelena
AU  - Klajn, Andrijana
AU  - Kovačević Grujičić, Nataša
AU  - Ma, Qing
AU  - Stevanović, Milena
AU  - Woloschak, Gayle E.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1167
PB  - Amer Assoc Cancer Research, Philadelphia
C3  - Cancer Research
T1  - WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin
IS  - 13
VL  - 78
DO  - 10.1158/1538-7445.AM2018-456
ER  - 

@conference{
author = "Paunesku, Tatjana and Popović, Jelena and Klajn, Andrijana and Kovačević Grujičić, Nataša and Ma, Qing and Stevanović, Milena and Woloschak, Gayle E.",
year = "2018",
publisher = "Amer Assoc Cancer Research, Philadelphia",
journal = "Cancer Research",
title = "WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin",
number = "13",
volume = "78",
doi = "10.1158/1538-7445.AM2018-456"
}

Paunesku, T., Popović, J., Klajn, A., Kovačević Grujičić, N., Ma, Q., Stevanović, M.,& Woloschak, G. E.. (2018). WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin. in Cancer Research
Amer Assoc Cancer Research, Philadelphia., 78(13).
https://doi.org/10.1158/1538-7445.AM2018-456

Paunesku T, Popović J, Klajn A, Kovačević Grujičić N, Ma Q, Stevanović M, Woloschak GE. WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin. in Cancer Research. 2018;78(13).
doi:10.1158/1538-7445.AM2018-456 .

Paunesku, Tatjana, Popović, Jelena, Klajn, Andrijana, Kovačević Grujičić, Nataša, Ma, Qing, Stevanović, Milena, Woloschak, Gayle E., "WR1065, the active metabolite of amifostine modulates chemistry and biology of cisplatin" in Cancer Research, 78, no. 13 (2018),
https://doi.org/10.1158/1538-7445.AM2018-456 . .

TY  - JOUR
AU  - Stojković, Dejan S.
AU  - Kovačević Grujičić, Nataša
AU  - Reis, Filipa S.
AU  - Davidović, Slobodan
AU  - Barros, Lillian
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Pavić, Aleksandar
AU  - Glamoclija, Jasmina
AU  - Cirić, Ana
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1051
AB  - Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with delta- and gamma-tocopherols being predominant (123.35 and 77.80 mu g/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 mu g/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125-5 mg/mL; MIC/MFC 0.025-0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (lC(50) 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.
PB  - Elsevier Science Bv, Amsterdam
T2  - Lwt-Food Science and Technology
T1  - Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract
EP  - 462
SP  - 454
VL  - 79
DO  - 10.1016/j.lwt.2017.01.045
ER  - 

@article{
author = "Stojković, Dejan S. and Kovačević Grujičić, Nataša and Reis, Filipa S. and Davidović, Slobodan and Barros, Lillian and Popović, Jelena and Petrović, Isidora and Pavić, Aleksandar and Glamoclija, Jasmina and Cirić, Ana and Stevanović, Milena and Ferreira, Isabel C. F. R. and Soković, Marina",
year = "2017",
abstract = "Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with delta- and gamma-tocopherols being predominant (123.35 and 77.80 mu g/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 mu g/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125-5 mg/mL; MIC/MFC 0.025-0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (lC(50) 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Lwt-Food Science and Technology",
title = "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract",
pages = "462-454",
volume = "79",
doi = "10.1016/j.lwt.2017.01.045"
}

Stojković, D. S., Kovačević Grujičić, N., Reis, F. S., Davidović, S., Barros, L., Popović, J., Petrović, I., Pavić, A., Glamoclija, J., Cirić, A., Stevanović, M., Ferreira, I. C. F. R.,& Soković, M.. (2017). Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in Lwt-Food Science and Technology
Elsevier Science Bv, Amsterdam., 79, 454-462.
https://doi.org/10.1016/j.lwt.2017.01.045

Stojković DS, Kovačević Grujičić N, Reis FS, Davidović S, Barros L, Popović J, Petrović I, Pavić A, Glamoclija J, Cirić A, Stevanović M, Ferreira ICFR, Soković M. Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in Lwt-Food Science and Technology. 2017;79:454-462.
doi:10.1016/j.lwt.2017.01.045 .

Stojković, Dejan S., Kovačević Grujičić, Nataša, Reis, Filipa S., Davidović, Slobodan, Barros, Lillian, Popović, Jelena, Petrović, Isidora, Pavić, Aleksandar, Glamoclija, Jasmina, Cirić, Ana, Stevanović, Milena, Ferreira, Isabel C. F. R., Soković, Marina, "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract" in Lwt-Food Science and Technology, 79 (2017):454-462,
https://doi.org/10.1016/j.lwt.2017.01.045 . .

TY  - JOUR
AU  - Vicentić, Jelena Marjanovic
AU  - Schwirtlich, Marija
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1007
AB  - Glioblastoma (GBM) is one of the most aggressive and deadly forms of cancer. Literature data reveals that all-trans retinoic acid (ATRA) has anticancer effects on different types of tumor cells. However, data about the effects of ATRA on glioblastoma cells are contradictory. In this study, we examined whether ATRA treatment affects features of human glioblastoma U251 cells. To that end, the cells were treated with different concentrations of ATRA. Results obtained by MTT and the crystal violet assays imply that ATRA affected the viability of U251 glioblastoma cells in a dose-and time-dependent manner. Fluorescence staining of microtubule cytoskeleton protein a-tubulin revealed that ATRA induced changes in cell morphology. Using semi-quantitative RT-PCR we found that the expression of SOX3 and GFAP genes, as markers of neural differentiation, was not changed upon ATRA treatment. Thus, the observed changes in cell morphology after ATRA treatment are not associated with neural differentiation of U251 glioblastoma cells. The scratch-wound healing assay revealed that ATRA changed the mode of U251 cell migration from collective to single cell motility. The cell-matrix adhesion assay demonstrated that the pharmacologically relevant concentration of ATRA lowered the cell-matrix adhesion capability of U251 cells. In conclusion, our results imply that further studies are needed before ATRA could be considered for the treatment of glioblastoma.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells
EP  - 706
IS  - 4
SP  - 699
VL  - 69
DO  - 10.2298/ABS170327016M
ER  - 

@article{
author = "Vicentić, Jelena Marjanovic and Schwirtlich, Marija and Kovačević Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela",
year = "2017",
abstract = "Glioblastoma (GBM) is one of the most aggressive and deadly forms of cancer. Literature data reveals that all-trans retinoic acid (ATRA) has anticancer effects on different types of tumor cells. However, data about the effects of ATRA on glioblastoma cells are contradictory. In this study, we examined whether ATRA treatment affects features of human glioblastoma U251 cells. To that end, the cells were treated with different concentrations of ATRA. Results obtained by MTT and the crystal violet assays imply that ATRA affected the viability of U251 glioblastoma cells in a dose-and time-dependent manner. Fluorescence staining of microtubule cytoskeleton protein a-tubulin revealed that ATRA induced changes in cell morphology. Using semi-quantitative RT-PCR we found that the expression of SOX3 and GFAP genes, as markers of neural differentiation, was not changed upon ATRA treatment. Thus, the observed changes in cell morphology after ATRA treatment are not associated with neural differentiation of U251 glioblastoma cells. The scratch-wound healing assay revealed that ATRA changed the mode of U251 cell migration from collective to single cell motility. The cell-matrix adhesion assay demonstrated that the pharmacologically relevant concentration of ATRA lowered the cell-matrix adhesion capability of U251 cells. In conclusion, our results imply that further studies are needed before ATRA could be considered for the treatment of glioblastoma.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells",
pages = "706-699",
number = "4",
volume = "69",
doi = "10.2298/ABS170327016M"
}

Vicentić, J. M., Schwirtlich, M., Kovačević Grujičić, N., Stevanović, M.,& Drakulić, D.. (2017). All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 69(4), 699-706.
https://doi.org/10.2298/ABS170327016M

Vicentić JM, Schwirtlich M, Kovačević Grujičić N, Stevanović M, Drakulić D. All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells. in Archives of Biological Sciences. 2017;69(4):699-706.
doi:10.2298/ABS170327016M .

Vicentić, Jelena Marjanovic, Schwirtlich, Marija, Kovačević Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, "All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells" in Archives of Biological Sciences, 69, no. 4 (2017):699-706,
https://doi.org/10.2298/ABS170327016M . .

TY  - JOUR
AU  - Podolski-Renić, Ana
AU  - Banković, Jasna
AU  - Dinić, Jelena
AU  - Rios-Luci, Carla
AU  - Fernandes, Miguel X.
AU  - Ortega, Nuria
AU  - Kovačević Grujičić, Nataša
AU  - Martin, Victor S.
AU  - Padron, Jose M.
AU  - Pesić, Milica
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1766
AB  - The efficacy of microtubule targeting agents in cancer treatment has been compromised by the development of drug resistance that may involve both, P-glycoprotein overexpression and the changes in beta-tubulin isoforms' expression. The anti-Topoisomerase II activity of methyl 4-((E)-2-(methoxycarbonyl)vinyloxy)oct-2-ynoate (DTA0100) was recently reported. Herein, we further evaluated this propargylic enol ether derivative and found that it exerts inhibitory effect on tubulin polymerization by binding to colchicine binding site. DTA0100 mitotic arrest properties were investigated in two multi-drug resistant cancer cell lines with P-glycoprotein overexpression (colorectal carcinoma and glioblastoma). The sensitivity of multi-drug resistant cancer cell lines to DTA0100 was not significantly changed in contrast to microtubule targeting agents such as paclitaxel, vinblastine and colchicine. DTA0100 clearly induced microtubule depolymerization, leading to disturbance of cell cycle kinetics and subsequent apoptosis. The fine-tuning in beta-tubulin isoforms expression observed in multi drug resistant cancer cells may influence the efficacy of DTA0100. Importantly, DTA0100 blocked the Pglycoprotein function in both multi-drug resistant cancer cell lines without inducing the increase in Pglycoprotein expression. Therefore, DTA0100 acting as dual inhibitor of Topoisomerase II and microtubule formation could be considered as multi-potent anticancer agent. Besides, it is able to overcome the problem of drug resistance that emerges in the therapeutic approaches with either Topoisomerase II or microtubule targeting agents.
PB  - Elsevier Science Bv, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - DTA0100, dual topoisomerase II and microtubule inhibitor, evades paclitaxel resistance in P-glycoprotein overexpressing cancer cells
EP  - 168
SP  - 159
VL  - 105
DO  - 10.1016/j.ejps.2017.05.011
ER  - 

@article{
author = "Podolski-Renić, Ana and Banković, Jasna and Dinić, Jelena and Rios-Luci, Carla and Fernandes, Miguel X. and Ortega, Nuria and Kovačević Grujičić, Nataša and Martin, Victor S. and Padron, Jose M. and Pesić, Milica",
year = "2017",
abstract = "The efficacy of microtubule targeting agents in cancer treatment has been compromised by the development of drug resistance that may involve both, P-glycoprotein overexpression and the changes in beta-tubulin isoforms' expression. The anti-Topoisomerase II activity of methyl 4-((E)-2-(methoxycarbonyl)vinyloxy)oct-2-ynoate (DTA0100) was recently reported. Herein, we further evaluated this propargylic enol ether derivative and found that it exerts inhibitory effect on tubulin polymerization by binding to colchicine binding site. DTA0100 mitotic arrest properties were investigated in two multi-drug resistant cancer cell lines with P-glycoprotein overexpression (colorectal carcinoma and glioblastoma). The sensitivity of multi-drug resistant cancer cell lines to DTA0100 was not significantly changed in contrast to microtubule targeting agents such as paclitaxel, vinblastine and colchicine. DTA0100 clearly induced microtubule depolymerization, leading to disturbance of cell cycle kinetics and subsequent apoptosis. The fine-tuning in beta-tubulin isoforms expression observed in multi drug resistant cancer cells may influence the efficacy of DTA0100. Importantly, DTA0100 blocked the Pglycoprotein function in both multi-drug resistant cancer cell lines without inducing the increase in Pglycoprotein expression. Therefore, DTA0100 acting as dual inhibitor of Topoisomerase II and microtubule formation could be considered as multi-potent anticancer agent. Besides, it is able to overcome the problem of drug resistance that emerges in the therapeutic approaches with either Topoisomerase II or microtubule targeting agents.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "DTA0100, dual topoisomerase II and microtubule inhibitor, evades paclitaxel resistance in P-glycoprotein overexpressing cancer cells",
pages = "168-159",
volume = "105",
doi = "10.1016/j.ejps.2017.05.011"
}

Podolski-Renić, A., Banković, J., Dinić, J., Rios-Luci, C., Fernandes, M. X., Ortega, N., Kovačević Grujičić, N., Martin, V. S., Padron, J. M.,& Pesić, M.. (2017). DTA0100, dual topoisomerase II and microtubule inhibitor, evades paclitaxel resistance in P-glycoprotein overexpressing cancer cells. in European Journal of Pharmaceutical Sciences
Elsevier Science Bv, Amsterdam., 105, 159-168.
https://doi.org/10.1016/j.ejps.2017.05.011

Podolski-Renić A, Banković J, Dinić J, Rios-Luci C, Fernandes MX, Ortega N, Kovačević Grujičić N, Martin VS, Padron JM, Pesić M. DTA0100, dual topoisomerase II and microtubule inhibitor, evades paclitaxel resistance in P-glycoprotein overexpressing cancer cells. in European Journal of Pharmaceutical Sciences. 2017;105:159-168.
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Podolski-Renić, Ana, Banković, Jasna, Dinić, Jelena, Rios-Luci, Carla, Fernandes, Miguel X., Ortega, Nuria, Kovačević Grujičić, Nataša, Martin, Victor S., Padron, Jose M., Pesić, Milica, "DTA0100, dual topoisomerase II and microtubule inhibitor, evades paclitaxel resistance in P-glycoprotein overexpressing cancer cells" in European Journal of Pharmaceutical Sciences, 105 (2017):159-168,
https://doi.org/10.1016/j.ejps.2017.05.011 . .