TY  - JOUR
AU  - Loncarić, Darija
AU  - Rodriguez, Laura
AU  - Debeissat, Christelle
AU  - Touya, Nicolas
AU  - Labat, Veronique
AU  - Villacreces, Arnaud
AU  - Bouzier-Sore, Anne-Karine
AU  - Pasquet, Jean-Max
AU  - de la Grange, Philippe Brunet
AU  - Vlaski-Lafarge, Marija
AU  - Pavlović, Sonja
AU  - Ivanović, Zoran
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1754
AB  - We present here the data showing, in standard cultures exposed to atmospheric O-2 concentration, that alpha-tocopherol acetate (alpha-TOA) has a positive impact on primitive cells inside mesenchymal stromal cell (MstroC) population, by maintaining their proliferative capacity. alpha-TOA decreases the O-2 consumption rate of MStroC probably by impacting respiratory chain complex II activity. This action, however, is not associated with a compensatory increase in glycolysis activity, in spite of the fact that the degradation of HIF-1 alpha was decreased in presence of alpha-TOA. This is in line with a moderate enhancement of mtROS upon alpha-TOA treatment. However, the absence of glycolysis stimulation implies the inactivity of HIF-1 alpha which might - if it were active - be related to the maintenance of stemness. It should be stressed that alpha-TOA might act directly on the gene expression as well as the mtROS themselves, which remains to be elucidated.
PB  - Springer, New York
T2  - Stem Cell Reviews and Reports
T1  - alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population
EP  - 1405
IS  - 4
SP  - 1390
VL  - 17
DO  - 10.1007/s12015-020-10111-9
ER  - 

@article{
author = "Loncarić, Darija and Rodriguez, Laura and Debeissat, Christelle and Touya, Nicolas and Labat, Veronique and Villacreces, Arnaud and Bouzier-Sore, Anne-Karine and Pasquet, Jean-Max and de la Grange, Philippe Brunet and Vlaski-Lafarge, Marija and Pavlović, Sonja and Ivanović, Zoran",
year = "2021",
abstract = "We present here the data showing, in standard cultures exposed to atmospheric O-2 concentration, that alpha-tocopherol acetate (alpha-TOA) has a positive impact on primitive cells inside mesenchymal stromal cell (MstroC) population, by maintaining their proliferative capacity. alpha-TOA decreases the O-2 consumption rate of MStroC probably by impacting respiratory chain complex II activity. This action, however, is not associated with a compensatory increase in glycolysis activity, in spite of the fact that the degradation of HIF-1 alpha was decreased in presence of alpha-TOA. This is in line with a moderate enhancement of mtROS upon alpha-TOA treatment. However, the absence of glycolysis stimulation implies the inactivity of HIF-1 alpha which might - if it were active - be related to the maintenance of stemness. It should be stressed that alpha-TOA might act directly on the gene expression as well as the mtROS themselves, which remains to be elucidated.",
publisher = "Springer, New York",
journal = "Stem Cell Reviews and Reports",
title = "alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population",
pages = "1405-1390",
number = "4",
volume = "17",
doi = "10.1007/s12015-020-10111-9"
}

Loncarić, D., Rodriguez, L., Debeissat, C., Touya, N., Labat, V., Villacreces, A., Bouzier-Sore, A., Pasquet, J., de la Grange, P. B., Vlaski-Lafarge, M., Pavlović, S.,& Ivanović, Z.. (2021). alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population. in Stem Cell Reviews and Reports
Springer, New York., 17(4), 1390-1405.
https://doi.org/10.1007/s12015-020-10111-9

Loncarić D, Rodriguez L, Debeissat C, Touya N, Labat V, Villacreces A, Bouzier-Sore A, Pasquet J, de la Grange PB, Vlaski-Lafarge M, Pavlović S, Ivanović Z. alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population. in Stem Cell Reviews and Reports. 2021;17(4):1390-1405.
doi:10.1007/s12015-020-10111-9 .

Loncarić, Darija, Rodriguez, Laura, Debeissat, Christelle, Touya, Nicolas, Labat, Veronique, Villacreces, Arnaud, Bouzier-Sore, Anne-Karine, Pasquet, Jean-Max, de la Grange, Philippe Brunet, Vlaski-Lafarge, Marija, Pavlović, Sonja, Ivanović, Zoran, "alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population" in Stem Cell Reviews and Reports, 17, no. 4 (2021):1390-1405,
https://doi.org/10.1007/s12015-020-10111-9 . .

TY  - JOUR
AU  - Loncarić, Darija
AU  - Rodriguez, Laura
AU  - Debeissat, Christelle
AU  - Touya, Nicolas
AU  - Labat, Veronique
AU  - Villacreces, Arnaud
AU  - Bouzier-Sore, Anne-Karine
AU  - Pasquet, Jean-Max
AU  - de la Grange, Philippe Brunet
AU  - Vlaski-Lafarge, Marija
AU  - Pavlović, Sonja
AU  - Ivanović, Zoran
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1491
AB  - We present here the data showing, in standard cultures exposed to atmospheric O-2 concentration, that alpha-tocopherol acetate (alpha-TOA) has a positive impact on primitive cells inside mesenchymal stromal cell (MstroC) population, by maintaining their proliferative capacity. alpha-TOA decreases the O-2 consumption rate of MStroC probably by impacting respiratory chain complex II activity. This action, however, is not associated with a compensatory increase in glycolysis activity, in spite of the fact that the degradation of HIF-1 alpha was decreased in presence of alpha-TOA. This is in line with a moderate enhancement of mtROS upon alpha-TOA treatment. However, the absence of glycolysis stimulation implies the inactivity of HIF-1 alpha which might - if it were active - be related to the maintenance of stemness. It should be stressed that alpha-TOA might act directly on the gene expression as well as the mtROS themselves, which remains to be elucidated.
PB  - Springer, New York
T2  - Stem Cell Reviews and Reports
T1  - alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population
EP  - 1405
IS  - 4
SP  - 1390
VL  - 17
DO  - 10.1007/s12015-020-10111-9
ER  - 

@article{
author = "Loncarić, Darija and Rodriguez, Laura and Debeissat, Christelle and Touya, Nicolas and Labat, Veronique and Villacreces, Arnaud and Bouzier-Sore, Anne-Karine and Pasquet, Jean-Max and de la Grange, Philippe Brunet and Vlaski-Lafarge, Marija and Pavlović, Sonja and Ivanović, Zoran",
year = "2021",
abstract = "We present here the data showing, in standard cultures exposed to atmospheric O-2 concentration, that alpha-tocopherol acetate (alpha-TOA) has a positive impact on primitive cells inside mesenchymal stromal cell (MstroC) population, by maintaining their proliferative capacity. alpha-TOA decreases the O-2 consumption rate of MStroC probably by impacting respiratory chain complex II activity. This action, however, is not associated with a compensatory increase in glycolysis activity, in spite of the fact that the degradation of HIF-1 alpha was decreased in presence of alpha-TOA. This is in line with a moderate enhancement of mtROS upon alpha-TOA treatment. However, the absence of glycolysis stimulation implies the inactivity of HIF-1 alpha which might - if it were active - be related to the maintenance of stemness. It should be stressed that alpha-TOA might act directly on the gene expression as well as the mtROS themselves, which remains to be elucidated.",
publisher = "Springer, New York",
journal = "Stem Cell Reviews and Reports",
title = "alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population",
pages = "1405-1390",
number = "4",
volume = "17",
doi = "10.1007/s12015-020-10111-9"
}

Loncarić, D., Rodriguez, L., Debeissat, C., Touya, N., Labat, V., Villacreces, A., Bouzier-Sore, A., Pasquet, J., de la Grange, P. B., Vlaski-Lafarge, M., Pavlović, S.,& Ivanović, Z.. (2021). alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population. in Stem Cell Reviews and Reports
Springer, New York., 17(4), 1390-1405.
https://doi.org/10.1007/s12015-020-10111-9

Loncarić D, Rodriguez L, Debeissat C, Touya N, Labat V, Villacreces A, Bouzier-Sore A, Pasquet J, de la Grange PB, Vlaski-Lafarge M, Pavlović S, Ivanović Z. alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population. in Stem Cell Reviews and Reports. 2021;17(4):1390-1405.
doi:10.1007/s12015-020-10111-9 .

Loncarić, Darija, Rodriguez, Laura, Debeissat, Christelle, Touya, Nicolas, Labat, Veronique, Villacreces, Arnaud, Bouzier-Sore, Anne-Karine, Pasquet, Jean-Max, de la Grange, Philippe Brunet, Vlaski-Lafarge, Marija, Pavlović, Sonja, Ivanović, Zoran, "alpha-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population" in Stem Cell Reviews and Reports, 17, no. 4 (2021):1390-1405,
https://doi.org/10.1007/s12015-020-10111-9 . .

TY  - JOUR
AU  - Loncarić, Darija
AU  - Stanković, Biljana
AU  - Ghousein, Amani
AU  - Vreca, Misa
AU  - Spasovski, Vesna
AU  - Villacreces, Arnaud
AU  - Debeissat, Christelle
AU  - Grosset, Christophe F.
AU  - Ivanović, Zoran
AU  - Pavlović, Sonja
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1263
AB  - A major limitation in the development of efficient clinical protocols for mesenchymal stromal cell (MStroC)-based tissue regeneration therapy is the low retention and survival of MStroC in injured tissue after therapeutic administration. Low oxygen concentration preconditioning (LOP) during ex vivo cultivation of MStroC, as a method for mimicking oxygenation in their physiological microenvironment, has been shown to be beneficial in clinical trials using MStroC. Introducing hypoxia-mimicking molecules into MStroC during cultivation could be an advantageous LOP strategy. MicroRNA (miRNA) drugs are good candidates for this approach. Analysis of the expression of miRNA-210 in human bone marrow-derived MStroC in conditions of acute and extended hypoxia (24 to 72 h) was performed using RT-qPCR methodology. HIF-1 alpha and HIF-2 alpha gene knockdown cell lines were generated using lentiviral transduction of short hairpin RNA (shRNA) in order to examine whether miRNA-210 expression is regulated by transcription factor HIF-1 and/or HIF-2. We detected a significant increase in miRNA-210 expression in hypoxic conditions at time points of 24, 48 and 72 h (p lt 0.05). Knocking down of HIF-1 alpha and HIF-2 alpha genes indicated involvement of both transcription factors in the elevation of miRNA-210 expression. These results point to miRNA-210 as a good candidate for a hypoxia-mimicking molecule in LOP strategy.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Expression of miRNA-210 in human bone marrow-derived mesenchymal stromal cells under oxygen deprivation
EP  - 208
IS  - 2
SP  - 201
VL  - 71
DO  - 10.2298/ABS181117001L
ER  - 

@article{
author = "Loncarić, Darija and Stanković, Biljana and Ghousein, Amani and Vreca, Misa and Spasovski, Vesna and Villacreces, Arnaud and Debeissat, Christelle and Grosset, Christophe F. and Ivanović, Zoran and Pavlović, Sonja",
year = "2019",
abstract = "A major limitation in the development of efficient clinical protocols for mesenchymal stromal cell (MStroC)-based tissue regeneration therapy is the low retention and survival of MStroC in injured tissue after therapeutic administration. Low oxygen concentration preconditioning (LOP) during ex vivo cultivation of MStroC, as a method for mimicking oxygenation in their physiological microenvironment, has been shown to be beneficial in clinical trials using MStroC. Introducing hypoxia-mimicking molecules into MStroC during cultivation could be an advantageous LOP strategy. MicroRNA (miRNA) drugs are good candidates for this approach. Analysis of the expression of miRNA-210 in human bone marrow-derived MStroC in conditions of acute and extended hypoxia (24 to 72 h) was performed using RT-qPCR methodology. HIF-1 alpha and HIF-2 alpha gene knockdown cell lines were generated using lentiviral transduction of short hairpin RNA (shRNA) in order to examine whether miRNA-210 expression is regulated by transcription factor HIF-1 and/or HIF-2. We detected a significant increase in miRNA-210 expression in hypoxic conditions at time points of 24, 48 and 72 h (p lt 0.05). Knocking down of HIF-1 alpha and HIF-2 alpha genes indicated involvement of both transcription factors in the elevation of miRNA-210 expression. These results point to miRNA-210 as a good candidate for a hypoxia-mimicking molecule in LOP strategy.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Expression of miRNA-210 in human bone marrow-derived mesenchymal stromal cells under oxygen deprivation",
pages = "208-201",
number = "2",
volume = "71",
doi = "10.2298/ABS181117001L"
}

Loncarić, D., Stanković, B., Ghousein, A., Vreca, M., Spasovski, V., Villacreces, A., Debeissat, C., Grosset, C. F., Ivanović, Z.,& Pavlović, S.. (2019). Expression of miRNA-210 in human bone marrow-derived mesenchymal stromal cells under oxygen deprivation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(2), 201-208.
https://doi.org/10.2298/ABS181117001L

Loncarić D, Stanković B, Ghousein A, Vreca M, Spasovski V, Villacreces A, Debeissat C, Grosset CF, Ivanović Z, Pavlović S. Expression of miRNA-210 in human bone marrow-derived mesenchymal stromal cells under oxygen deprivation. in Archives of Biological Sciences. 2019;71(2):201-208.
doi:10.2298/ABS181117001L .

Loncarić, Darija, Stanković, Biljana, Ghousein, Amani, Vreca, Misa, Spasovski, Vesna, Villacreces, Arnaud, Debeissat, Christelle, Grosset, Christophe F., Ivanović, Zoran, Pavlović, Sonja, "Expression of miRNA-210 in human bone marrow-derived mesenchymal stromal cells under oxygen deprivation" in Archives of Biological Sciences, 71, no. 2 (2019):201-208,
https://doi.org/10.2298/ABS181117001L . .