TY  - CONF
AU  - Atanasković, Marija
AU  - Morić, Ivana
AU  - B. Rokić, Miloš
AU  - Đokić, Anđela
AU  - Pantović, Jelena
AU  - Despotović, Dragana
AU  - Šenerović, Lidija
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2369
AB  - The formation of biofilms by foodborne pathogens
is a constant challenge in the food industry,
leading to an increased risk of contamination and
compromising food safety. Many of the chemicals
commonly used for sanitation in the food industry
are unable to remove biofilms, are harmful
to surfaces and can be toxic. The effectiveness
of disinfectants can be improved using enzymes
that specifically target biofilm components such
as exopolysaccharides, extracellular DNA, or proteins.
In this study we investigated the potential
of glycoside hydrolases originating from the
gill microbiota of freshwater fish to control biofilm
formation in the most common foodborne
pathogens. We demonstrated that β-glucosidase
from Microbacterium sp. BG28 (BglB-BG28) effectively
inhibits cellulose-rich biofilms formed by
Salmonella enteritidis, S. typhimurium, S. infantis,
and Escherichia coli. When these bacteria were cultivated overnight with 200 μL/mL enzyme, up
to 80% less biofilm was formed. By fluorescence
microscopy, we visualised the inhibition of biofilms
on plastic, glass and aluminium, materials
commonly used in the food industry. When used
as a pre-treatment, BglB-BG28 increased the
bactericidal efficacy of Oxicid®S, a commercially
available surface disinfectant. Its effectiveness at
temperatures up to 50 °C and in a pH range from
4 to 8 together with compatibility with non-ionic
detergents and high tolerance to sodium chloride
and glucose give BglB-BG28 advantages in
harsh and diverse industrial environments. Importantly,
no toxicity to Caenorhabditis elegans
was observed at enzyme concentrations of up
to 1 mg/ml. Overall, these results demonstrate
the suitability of the β-glucosidase BglB-BG28 for
the formulation of a novel enzyme-based disinfectant
to be used in food processing facilities.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - GLYCOSIDE HYDROLASES FROM FRESHWATER FISH GILL MICROBIOTA AS BIOFILM INHIBITORS FOR ENHANCED FOOD SAFETY
EP  - 42
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2369
ER  - 

@conference{
author = "Atanasković, Marija and Morić, Ivana and B. Rokić, Miloš and Đokić, Anđela and Pantović, Jelena and Despotović, Dragana and Šenerović, Lidija",
year = "2024",
abstract = "The formation of biofilms by foodborne pathogens
is a constant challenge in the food industry,
leading to an increased risk of contamination and
compromising food safety. Many of the chemicals
commonly used for sanitation in the food industry
are unable to remove biofilms, are harmful
to surfaces and can be toxic. The effectiveness
of disinfectants can be improved using enzymes
that specifically target biofilm components such
as exopolysaccharides, extracellular DNA, or proteins.
In this study we investigated the potential
of glycoside hydrolases originating from the
gill microbiota of freshwater fish to control biofilm
formation in the most common foodborne
pathogens. We demonstrated that β-glucosidase
from Microbacterium sp. BG28 (BglB-BG28) effectively
inhibits cellulose-rich biofilms formed by
Salmonella enteritidis, S. typhimurium, S. infantis,
and Escherichia coli. When these bacteria were cultivated overnight with 200 μL/mL enzyme, up
to 80% less biofilm was formed. By fluorescence
microscopy, we visualised the inhibition of biofilms
on plastic, glass and aluminium, materials
commonly used in the food industry. When used
as a pre-treatment, BglB-BG28 increased the
bactericidal efficacy of Oxicid®S, a commercially
available surface disinfectant. Its effectiveness at
temperatures up to 50 °C and in a pH range from
4 to 8 together with compatibility with non-ionic
detergents and high tolerance to sodium chloride
and glucose give BglB-BG28 advantages in
harsh and diverse industrial environments. Importantly,
no toxicity to Caenorhabditis elegans
was observed at enzyme concentrations of up
to 1 mg/ml. Overall, these results demonstrate
the suitability of the β-glucosidase BglB-BG28 for
the formulation of a novel enzyme-based disinfectant
to be used in food processing facilities.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "GLYCOSIDE HYDROLASES FROM FRESHWATER FISH GILL MICROBIOTA AS BIOFILM INHIBITORS FOR ENHANCED FOOD SAFETY",
pages = "42-42",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2369"
}

Atanasković, M., Morić, I., B. Rokić, M., Đokić, A., Pantović, J., Despotović, D.,& Šenerović, L.. (2024). GLYCOSIDE HYDROLASES FROM FRESHWATER FISH GILL MICROBIOTA AS BIOFILM INHIBITORS FOR ENHANCED FOOD SAFETY. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 42-42.
https://hdl.handle.net/21.15107/rcub_imagine_2369

Atanasković M, Morić I, B. Rokić M, Đokić A, Pantović J, Despotović D, Šenerović L. GLYCOSIDE HYDROLASES FROM FRESHWATER FISH GILL MICROBIOTA AS BIOFILM INHIBITORS FOR ENHANCED FOOD SAFETY. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:42-42.
https://hdl.handle.net/21.15107/rcub_imagine_2369 .

Atanasković, Marija, Morić, Ivana, B. Rokić, Miloš, Đokić, Anđela, Pantović, Jelena, Despotović, Dragana, Šenerović, Lidija, "GLYCOSIDE HYDROLASES FROM FRESHWATER FISH GILL MICROBIOTA AS BIOFILM INHIBITORS FOR ENHANCED FOOD SAFETY" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):42-42,
https://hdl.handle.net/21.15107/rcub_imagine_2369 .

TY  - JOUR
AU  - Virijević, Katarina
AU  - Živanović, Marko N.
AU  - Nikolić, Dalibor
AU  - Milivojević, Nevena
AU  - Pavić, Jelena
AU  - Morić, Ivana
AU  - Šenerović, Lidija
AU  - Dragačević, Luka
AU  - Thurner, Philipp J.
AU  - Rufin, Manuel
AU  - Andriotis, Orestis G.
AU  - Ljujić, Biljana
AU  - Miletić Kovačević, Marina
AU  - Papić, Miloš
AU  - Filipović, Nenad
PY  - 2024
UR  - https://doi.org/10.1021/acsami.4c03266
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2360
AB  - Here, an artificial intelligence (AI)-based approach was employed to optimize the production of electrospun scaffolds for in vivo wound healing applications. By combining polycaprolactone (PCL) and poly(ethylene glycol) (PEG) in various concentration ratios, dissolved in chloroform (CHCl3) and dimethylformamide (DMF), 125 different polymer combinations were created. From these polymer combinations, electrospun nanofiber meshes were produced and characterized structurally and mechanically via microscopic techniques, including chemical composition and fiber diameter determination. Subsequently, these data were used to train a neural network, creating an AI model to predict the optimal scaffold production solution. Guided by the predictions and experimental outcomes of the AI model, the most promising scaffold for further in vitro analyses was identified. Moreover, we enriched this selected polymer combination by incorporating antibiotics, aiming to develop electrospun nanofiber scaffolds tailored for in vivo wound healing applications. Our study underscores three noteworthy conclusions: (i) the application of AI is pivotal in the fields of material and biomedical sciences, (ii) our methodology provides an effective blueprint for the initial screening of biomedical materials, and (iii) electrospun PCL/PEG antibiotic-bearing scaffolds exhibit outstanding results in promoting neoangiogenesis and facilitating in vivo wound treatment.
PB  - American Chemical Society
T2  - ACS Applied Materials & Interfaces
T1  - AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing
DO  - 10.1021/acsami.4c03266
ER  - 

@article{
author = "Virijević, Katarina and Živanović, Marko N. and Nikolić, Dalibor and Milivojević, Nevena and Pavić, Jelena and Morić, Ivana and Šenerović, Lidija and Dragačević, Luka and Thurner, Philipp J. and Rufin, Manuel and Andriotis, Orestis G. and Ljujić, Biljana and Miletić Kovačević, Marina and Papić, Miloš and Filipović, Nenad",
year = "2024",
abstract = "Here, an artificial intelligence (AI)-based approach was employed to optimize the production of electrospun scaffolds for in vivo wound healing applications. By combining polycaprolactone (PCL) and poly(ethylene glycol) (PEG) in various concentration ratios, dissolved in chloroform (CHCl3) and dimethylformamide (DMF), 125 different polymer combinations were created. From these polymer combinations, electrospun nanofiber meshes were produced and characterized structurally and mechanically via microscopic techniques, including chemical composition and fiber diameter determination. Subsequently, these data were used to train a neural network, creating an AI model to predict the optimal scaffold production solution. Guided by the predictions and experimental outcomes of the AI model, the most promising scaffold for further in vitro analyses was identified. Moreover, we enriched this selected polymer combination by incorporating antibiotics, aiming to develop electrospun nanofiber scaffolds tailored for in vivo wound healing applications. Our study underscores three noteworthy conclusions: (i) the application of AI is pivotal in the fields of material and biomedical sciences, (ii) our methodology provides an effective blueprint for the initial screening of biomedical materials, and (iii) electrospun PCL/PEG antibiotic-bearing scaffolds exhibit outstanding results in promoting neoangiogenesis and facilitating in vivo wound treatment.",
publisher = "American Chemical Society",
journal = "ACS Applied Materials & Interfaces",
title = "AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing",
doi = "10.1021/acsami.4c03266"
}

Virijević, K., Živanović, M. N., Nikolić, D., Milivojević, N., Pavić, J., Morić, I., Šenerović, L., Dragačević, L., Thurner, P. J., Rufin, M., Andriotis, O. G., Ljujić, B., Miletić Kovačević, M., Papić, M.,& Filipović, N.. (2024). AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. in ACS Applied Materials & Interfaces
American Chemical Society..
https://doi.org/10.1021/acsami.4c03266

Virijević K, Živanović MN, Nikolić D, Milivojević N, Pavić J, Morić I, Šenerović L, Dragačević L, Thurner PJ, Rufin M, Andriotis OG, Ljujić B, Miletić Kovačević M, Papić M, Filipović N. AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. in ACS Applied Materials & Interfaces. 2024;.
doi:10.1021/acsami.4c03266 .

Virijević, Katarina, Živanović, Marko N., Nikolić, Dalibor, Milivojević, Nevena, Pavić, Jelena, Morić, Ivana, Šenerović, Lidija, Dragačević, Luka, Thurner, Philipp J., Rufin, Manuel, Andriotis, Orestis G., Ljujić, Biljana, Miletić Kovačević, Marina, Papić, Miloš, Filipović, Nenad, "AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing" in ACS Applied Materials & Interfaces (2024),
https://doi.org/10.1021/acsami.4c03266 . .

TY  - JOUR
AU  - Atanasković, Marija
AU  - Morić, Ivana
AU  - Rokić, Miloš
AU  - Đokić, Anđela
AU  - Pantović, Jelena
AU  - Despotović, Dragana
AU  - Šenerović, Lidija
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S221242922301194X
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2331
AB  - SalmonellaEnteritidis is the most commonly reported pathogen for foodborne illness outbreaks in both underdeveloped and developed regions. S. Enteritidis biofilms, which form on various food contact surfaces, are resistant to conventional physical and chemical cleaning and disinfection procedures routinely used in food processing. The aim of this study was to identify novel, industrially applicable enzymes that are active against S. Enteritidis biofilms. We describe the properties and anti-biofilm activity of heterologously expressed β-glucosidase B derived from the environmental strain Microbacterium sp. BG28 (BglB-BG28) collected from gills of bream fish. The enzyme inhibited adhesion and the early stages of biofilm formation in clinical isolates of S. Enteritidis. At a concentration of 200 μg/mL, BglB-BG28 effectively reduced biofilm formation, by decreasing biofilm biomass by 50% and metabolic activity within biofilms by 80%. The enzyme reduced the formation of air-liquid biofilms on various surfaces, including plastic, glass and metal, as observed by fluorescence microscopy. BglB-BG28 inhibited biofilm formation in Escherichia coli, another important food pathogen that also forms cellulose-rich biofilms. Using o-NPG as substrate, the enzyme showed activity at temperatures up to 50 °C and in a pH range between 4 and 8, high tolerance to sodium chloride and glucose, and compatibility with nonionic detergents. Importantly, no toxicity was observed in the model system Caenorhabditis elegans even at an enzyme concentration of 1 mg/mL. These results suggest that the β-glucosidase BglB-BG28 is a promising candidate for the development of a new enzyme-based disinfection protocol that can be used in food processing facilities.
PB  - Elsevier
T2  - Food Bioscience
T2  - Food BioscienceFood Bioscience
T1  - Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28
SP  - 103543
VL  - 57
DO  - 10.1016/j.fbio.2023.103543
ER  - 

@article{
author = "Atanasković, Marija and Morić, Ivana and Rokić, Miloš and Đokić, Anđela and Pantović, Jelena and Despotović, Dragana and Šenerović, Lidija",
year = "2024",
abstract = "SalmonellaEnteritidis is the most commonly reported pathogen for foodborne illness outbreaks in both underdeveloped and developed regions. S. Enteritidis biofilms, which form on various food contact surfaces, are resistant to conventional physical and chemical cleaning and disinfection procedures routinely used in food processing. The aim of this study was to identify novel, industrially applicable enzymes that are active against S. Enteritidis biofilms. We describe the properties and anti-biofilm activity of heterologously expressed β-glucosidase B derived from the environmental strain Microbacterium sp. BG28 (BglB-BG28) collected from gills of bream fish. The enzyme inhibited adhesion and the early stages of biofilm formation in clinical isolates of S. Enteritidis. At a concentration of 200 μg/mL, BglB-BG28 effectively reduced biofilm formation, by decreasing biofilm biomass by 50% and metabolic activity within biofilms by 80%. The enzyme reduced the formation of air-liquid biofilms on various surfaces, including plastic, glass and metal, as observed by fluorescence microscopy. BglB-BG28 inhibited biofilm formation in Escherichia coli, another important food pathogen that also forms cellulose-rich biofilms. Using o-NPG as substrate, the enzyme showed activity at temperatures up to 50 °C and in a pH range between 4 and 8, high tolerance to sodium chloride and glucose, and compatibility with nonionic detergents. Importantly, no toxicity was observed in the model system Caenorhabditis elegans even at an enzyme concentration of 1 mg/mL. These results suggest that the β-glucosidase BglB-BG28 is a promising candidate for the development of a new enzyme-based disinfection protocol that can be used in food processing facilities.",
publisher = "Elsevier",
journal = "Food Bioscience, Food BioscienceFood Bioscience",
title = "Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28",
pages = "103543",
volume = "57",
doi = "10.1016/j.fbio.2023.103543"
}

Atanasković, M., Morić, I., Rokić, M., Đokić, A., Pantović, J., Despotović, D.,& Šenerović, L.. (2024). Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28. in Food Bioscience
Elsevier., 57, 103543.
https://doi.org/10.1016/j.fbio.2023.103543

Atanasković M, Morić I, Rokić M, Đokić A, Pantović J, Despotović D, Šenerović L. Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28. in Food Bioscience. 2024;57:103543.
doi:10.1016/j.fbio.2023.103543 .

Atanasković, Marija, Morić, Ivana, Rokić, Miloš, Đokić, Anđela, Pantović, Jelena, Despotović, Dragana, Šenerović, Lidija, "Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28" in Food Bioscience, 57 (2024):103543,
https://doi.org/10.1016/j.fbio.2023.103543 . .

TY  - CONF
AU  - Đokić, Lidija
AU  - Rokić, Miloš
AU  - Šenerović, Lidija
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2376
AB  - The rapid emergence and spread of multidrug-
resistant pathogens present a global
healthcare challenge. One common cause of
resistance and/or tolerance to antibiotics is
biofilms, a complex communities of bacteria
embedded in a self-produced matrix. Biofilm
formation and maturation are regulated by
quorum sensing, a cell density-dependent communication
system that relies on the synthesis,
diffusion, and detection of small signaling molecules
- autoinducers (AIs). Quorum quenching
(QQ) enzymes that cut Ais emerged as a promising
strategy for persistent bacterial infections.
However, a significant drawback for the use of
QQ enzymes as therapeutics is their poor stability
and efficacy in vivo. Since one of the major
health issues linked to biofilm development is
persistent wound infections, our goal was to
improve enzyme properties by immobilizing it
on a natural biopolymer to make it suitable for
use as a wound dressing. The best candidate for immobilization was YtnP lactonase from Bacillus
paralicheniformis ZP1, as in concentrations
higher than 25 μg/mL it improved the survival of
Pseudomonas aeruginosa PAO1-infected zebrafish,
rescuing 80% of embryos. When combined
with tobramycin or gentamicin, the survival
rate of zebrafish embryos increased to 100%.
Purified YtnP lactonase at a concentration of 1
mg was immobilized on 10 mg of polymer disks
by crosslinking with glutaraldehyde. Specific
modifications of the polymer were also made to
eliminate the use of glutaraldehyde, which is a
skin irritant. In in vivo experiments on a murine
chronic wound model, immobilized enzyme
inhibited biofilm development, cleared already
formed biofilms, and overall improved wound
healing. These results provide a foundation for
the development of advanced wound dressings
that will prevent infection development in
wounds and enable proper therapy for infected
chronic wounds.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS
EP  - 126
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2376
ER  - 

@conference{
author = "Đokić, Lidija and Rokić, Miloš and Šenerović, Lidija",
year = "2024",
abstract = "The rapid emergence and spread of multidrug-
resistant pathogens present a global
healthcare challenge. One common cause of
resistance and/or tolerance to antibiotics is
biofilms, a complex communities of bacteria
embedded in a self-produced matrix. Biofilm
formation and maturation are regulated by
quorum sensing, a cell density-dependent communication
system that relies on the synthesis,
diffusion, and detection of small signaling molecules
- autoinducers (AIs). Quorum quenching
(QQ) enzymes that cut Ais emerged as a promising
strategy for persistent bacterial infections.
However, a significant drawback for the use of
QQ enzymes as therapeutics is their poor stability
and efficacy in vivo. Since one of the major
health issues linked to biofilm development is
persistent wound infections, our goal was to
improve enzyme properties by immobilizing it
on a natural biopolymer to make it suitable for
use as a wound dressing. The best candidate for immobilization was YtnP lactonase from Bacillus
paralicheniformis ZP1, as in concentrations
higher than 25 μg/mL it improved the survival of
Pseudomonas aeruginosa PAO1-infected zebrafish,
rescuing 80% of embryos. When combined
with tobramycin or gentamicin, the survival
rate of zebrafish embryos increased to 100%.
Purified YtnP lactonase at a concentration of 1
mg was immobilized on 10 mg of polymer disks
by crosslinking with glutaraldehyde. Specific
modifications of the polymer were also made to
eliminate the use of glutaraldehyde, which is a
skin irritant. In in vivo experiments on a murine
chronic wound model, immobilized enzyme
inhibited biofilm development, cleared already
formed biofilms, and overall improved wound
healing. These results provide a foundation for
the development of advanced wound dressings
that will prevent infection development in
wounds and enable proper therapy for infected
chronic wounds.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS",
pages = "126-126",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2376"
}

Đokić, L., Rokić, M.,& Šenerović, L.. (2024). NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 126-126.
https://hdl.handle.net/21.15107/rcub_imagine_2376

Đokić L, Rokić M, Šenerović L. NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:126-126.
https://hdl.handle.net/21.15107/rcub_imagine_2376 .

Đokić, Lidija, Rokić, Miloš, Šenerović, Lidija, "NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):126-126,
https://hdl.handle.net/21.15107/rcub_imagine_2376 .

TY  - CONF
AU  - Atanasković, Marija
AU  - Morić, Ivana
AU  - Rokić, Miloš
AU  - Šenerović, Lidija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2031
AB  - Biofilms are ubiquitous in nature, and the food industry is vulnerable to the risks posed by
biofilm formation. Not only do they interfere with the food production process, but they also
pose a public health threat. However, complete elimination of biofilms on food and food
contact surfaces cannot be achieved by conventional methods (cleaning and disinfection)
alone. New biofilm control strategies must be developed to prevent its formation and/or
persistence. Novel approaches may be based on enzymes that depolymerize components
of the biofilm matrix, making bacterial cells accessible to antimicrobial agents.
Environmental microorganisms are an inexhaustible source of new enzymes. In
Salmonella Enteritidis and Escherichia coli, known foodborne pathogens, cellulose is an
important component of the biofilm matrix, so our isolates from untapped environments
were tested for cellulolytic activity. Of the more than 70 isolates examined, isolate BG28
was selected as the most promising. Its genome was sequenced, annotated, and it was
identified as Gram-positive Microbacterium sp. Genome mining revealed the presence of
four complete genes for different β-glucosidases, one of three enzyme types of cellulase
complexes. To select the best candidate for heterologous expression DeepTMHMM,
ProtParam, and SoluProt were used to predict the presence/absence of signal peptide
and transmembrane domains, instability index, aliphatic index, hydrophilicity, and soluble
expression in E. coli. Based on the prediction results, the gene annotated as β-glucosidase
B was selected for recombinant expression. In addition, I-TASSER was used to model the
tertiary structure of the selected enzyme.
The β-glucosidase B was recombinantly expressed, purified, and tested for its anti-biofilm
activity. It was active and showed a 50% inhibitory effect on S. Enteritidis and E. coli biofilm
formation at a concentration of 100 μg/ml. To further evaluate this in silico approach in
the preselection of candidate enzymes for recombinant expression and purification, we
will use it to identify other enzymes of the cellulase complex.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - In silico pre-selection of β-glucosidase gene for heterologous recombinant expression
EP  - 86
SP  - 86
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2031
ER  - 

@conference{
author = "Atanasković, Marija and Morić, Ivana and Rokić, Miloš and Šenerović, Lidija",
year = "2023",
abstract = "Biofilms are ubiquitous in nature, and the food industry is vulnerable to the risks posed by
biofilm formation. Not only do they interfere with the food production process, but they also
pose a public health threat. However, complete elimination of biofilms on food and food
contact surfaces cannot be achieved by conventional methods (cleaning and disinfection)
alone. New biofilm control strategies must be developed to prevent its formation and/or
persistence. Novel approaches may be based on enzymes that depolymerize components
of the biofilm matrix, making bacterial cells accessible to antimicrobial agents.
Environmental microorganisms are an inexhaustible source of new enzymes. In
Salmonella Enteritidis and Escherichia coli, known foodborne pathogens, cellulose is an
important component of the biofilm matrix, so our isolates from untapped environments
were tested for cellulolytic activity. Of the more than 70 isolates examined, isolate BG28
was selected as the most promising. Its genome was sequenced, annotated, and it was
identified as Gram-positive Microbacterium sp. Genome mining revealed the presence of
four complete genes for different β-glucosidases, one of three enzyme types of cellulase
complexes. To select the best candidate for heterologous expression DeepTMHMM,
ProtParam, and SoluProt were used to predict the presence/absence of signal peptide
and transmembrane domains, instability index, aliphatic index, hydrophilicity, and soluble
expression in E. coli. Based on the prediction results, the gene annotated as β-glucosidase
B was selected for recombinant expression. In addition, I-TASSER was used to model the
tertiary structure of the selected enzyme.
The β-glucosidase B was recombinantly expressed, purified, and tested for its anti-biofilm
activity. It was active and showed a 50% inhibitory effect on S. Enteritidis and E. coli biofilm
formation at a concentration of 100 μg/ml. To further evaluate this in silico approach in
the preselection of candidate enzymes for recombinant expression and purification, we
will use it to identify other enzymes of the cellulase complex.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "In silico pre-selection of β-glucosidase gene for heterologous recombinant expression",
pages = "86-86",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2031"
}

Atanasković, M., Morić, I., Rokić, M.,& Šenerović, L.. (2023). In silico pre-selection of β-glucosidase gene for heterologous recombinant expression. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 86-86.
https://hdl.handle.net/21.15107/rcub_imagine_2031

Atanasković M, Morić I, Rokić M, Šenerović L. In silico pre-selection of β-glucosidase gene for heterologous recombinant expression. in 4th Belgrade Bioinformatics Conference. 2023;4:86-86.
https://hdl.handle.net/21.15107/rcub_imagine_2031 .

Atanasković, Marija, Morić, Ivana, Rokić, Miloš, Šenerović, Lidija, "In silico pre-selection of β-glucosidase gene for heterologous recombinant expression" in 4th Belgrade Bioinformatics Conference, 4 (2023):86-86,
https://hdl.handle.net/21.15107/rcub_imagine_2031 .

TY  - CONF
AU  - Đokić, Anđela
AU  - Morić, Ivana
AU  - Šenerović, Lidija
AU  - Đokić, Lidija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2047
AB  - Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe chronic
infections due to its exceptional ability to form a biofilm. Regulation of biofilm formation
is very sophisticated and involves multiple bacterial systems and regulatory pathways.
We found an enzyme MhqO dioxygenase from Bacillus paralicheniformis ZP1, which
was effective in the inhibition of biofilm formation and disruption of mature biofilm
of P. aeruginosa. Our results suggest that MhqO exerts its effect at the adhesion level,
preventing cells from attaching to the surface. We have also shown that the enzyme
stimulates the rhamnolipids synthesis.
To elucidate the mechanism of enzyme action, we analyzed the transcriptome of the P.
aeruginosa PAO1 strain treated with MhqO. Since cell adhesion occurs at the beginning
of the stationary phase growth, the PAO1 strain was treated with MhqO for four hours,
followed by total RNA isolation and cDNA synthesis. Transcriptome sequencing was
performed by Illumina NovaSeq 6000 and data were analyzed by Novogene Bioinformatics
Technology Co., Ltd. (Beijing, China).
Obtained data showed that 122 genes were up-regulated, 41 genes were down-regulated,
and the expression of 5947 genes was not changed. Five genes whose expression was
altered are directly related to biofilm formation. MhqO increased the expression of the
RsmA post-transcriptional regulator in P. aeruginosa. Transcriptome data revealed that pili
IV biosynthesis genes were up-regulated, which is in accordance with literature data that
RsmA positively regulates these genes. The inhibition of cells’ attachment to the surface
could be explained by these results. In addition, RsmA positively regulates rhamnolipid
production but negatively regulates biofilm matrix synthesis, which was supported by
expression levels in the sequenced transcriptome.
Data obtained from transcriptome analysis suggest that P. aeruginosa treated with MhqO
dioxygenase should be more sensitive to oxidative and osmotic stress, as well as to
beta-lactam antibiotics. Our further investigations should confirm these effects at the
phenotypic level as well.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Transcriptome analysis of Pseudomonas aeruginosa after MhqO dioxygenase treatment
EP  - 102
SP  - 102
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2047
ER  - 

@conference{
author = "Đokić, Anđela and Morić, Ivana and Šenerović, Lidija and Đokić, Lidija",
year = "2023",
abstract = "Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe chronic
infections due to its exceptional ability to form a biofilm. Regulation of biofilm formation
is very sophisticated and involves multiple bacterial systems and regulatory pathways.
We found an enzyme MhqO dioxygenase from Bacillus paralicheniformis ZP1, which
was effective in the inhibition of biofilm formation and disruption of mature biofilm
of P. aeruginosa. Our results suggest that MhqO exerts its effect at the adhesion level,
preventing cells from attaching to the surface. We have also shown that the enzyme
stimulates the rhamnolipids synthesis.
To elucidate the mechanism of enzyme action, we analyzed the transcriptome of the P.
aeruginosa PAO1 strain treated with MhqO. Since cell adhesion occurs at the beginning
of the stationary phase growth, the PAO1 strain was treated with MhqO for four hours,
followed by total RNA isolation and cDNA synthesis. Transcriptome sequencing was
performed by Illumina NovaSeq 6000 and data were analyzed by Novogene Bioinformatics
Technology Co., Ltd. (Beijing, China).
Obtained data showed that 122 genes were up-regulated, 41 genes were down-regulated,
and the expression of 5947 genes was not changed. Five genes whose expression was
altered are directly related to biofilm formation. MhqO increased the expression of the
RsmA post-transcriptional regulator in P. aeruginosa. Transcriptome data revealed that pili
IV biosynthesis genes were up-regulated, which is in accordance with literature data that
RsmA positively regulates these genes. The inhibition of cells’ attachment to the surface
could be explained by these results. In addition, RsmA positively regulates rhamnolipid
production but negatively regulates biofilm matrix synthesis, which was supported by
expression levels in the sequenced transcriptome.
Data obtained from transcriptome analysis suggest that P. aeruginosa treated with MhqO
dioxygenase should be more sensitive to oxidative and osmotic stress, as well as to
beta-lactam antibiotics. Our further investigations should confirm these effects at the
phenotypic level as well.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Transcriptome analysis of Pseudomonas aeruginosa after MhqO dioxygenase treatment",
pages = "102-102",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2047"
}

Đokić, A., Morić, I., Šenerović, L.,& Đokić, L.. (2023). Transcriptome analysis of Pseudomonas aeruginosa after MhqO dioxygenase treatment. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 102-102.
https://hdl.handle.net/21.15107/rcub_imagine_2047

Đokić A, Morić I, Šenerović L, Đokić L. Transcriptome analysis of Pseudomonas aeruginosa after MhqO dioxygenase treatment. in 4th Belgrade Bioinformatics Conference. 2023;4:102-102.
https://hdl.handle.net/21.15107/rcub_imagine_2047 .

Đokić, Anđela, Morić, Ivana, Šenerović, Lidija, Đokić, Lidija, "Transcriptome analysis of Pseudomonas aeruginosa after MhqO dioxygenase treatment" in 4th Belgrade Bioinformatics Conference, 4 (2023):102-102,
https://hdl.handle.net/21.15107/rcub_imagine_2047 .

TY  - JOUR
AU  - Joncić Savić, Katarina
AU  - Đokic, Lidija
AU  - Stanković, Nada
AU  - Morić, Ivana
AU  - Pavlović, Bojan
AU  - Šenerović, Lidija
AU  - Aydogan, Cem
AU  - Pavić, Aleksandar
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S1756464623002177
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1982
AB  - Candidiasis caused by Candida albicans is one of the most common fungal infections in modern society. The limited arsenal of clinical drugs, their side effects and emerging resistance, largely contribute to the low efficacy of current antifungal therapies. The morphogenetic yeast-to-hyphae transition is the key virulence feature for the establishment of local and systemic C. albicans infections. We show that a delfinidin-rich fruit extract from Aristotelia chilensis ([Molina], Stuntz) (maqui berry) inhibits filamentation of C. albicans, in both laboratory and clinical strains. The extract acts synergistically with nystatin, with filament formation completely prevented by the combination of ¼×MIC of nystatin and 0.125 mg/ml of the extract. The combination treatment results in increased survival of C. albicans-infected zebrafish embryos compared to treatment with nystatin. Neither the extract nor its combination with nystatin was toxic at effective doses. These results warrant further investigation of maqui berry extract as adjuvant antifungal treatment.
T2  - Journal of Functional Foods
T1  - Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin
SP  - 105617
VL  - 106
DO  - 10.1016/j.jff.2023.105617
ER  - 

@article{
author = "Joncić Savić, Katarina and Đokic, Lidija and Stanković, Nada and Morić, Ivana and Pavlović, Bojan and Šenerović, Lidija and Aydogan, Cem and Pavić, Aleksandar",
year = "2023",
abstract = "Candidiasis caused by Candida albicans is one of the most common fungal infections in modern society. The limited arsenal of clinical drugs, their side effects and emerging resistance, largely contribute to the low efficacy of current antifungal therapies. The morphogenetic yeast-to-hyphae transition is the key virulence feature for the establishment of local and systemic C. albicans infections. We show that a delfinidin-rich fruit extract from Aristotelia chilensis ([Molina], Stuntz) (maqui berry) inhibits filamentation of C. albicans, in both laboratory and clinical strains. The extract acts synergistically with nystatin, with filament formation completely prevented by the combination of ¼×MIC of nystatin and 0.125 mg/ml of the extract. The combination treatment results in increased survival of C. albicans-infected zebrafish embryos compared to treatment with nystatin. Neither the extract nor its combination with nystatin was toxic at effective doses. These results warrant further investigation of maqui berry extract as adjuvant antifungal treatment.",
journal = "Journal of Functional Foods",
title = "Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin",
pages = "105617",
volume = "106",
doi = "10.1016/j.jff.2023.105617"
}

Joncić Savić, K., Đokic, L., Stanković, N., Morić, I., Pavlović, B., Šenerović, L., Aydogan, C.,& Pavić, A.. (2023). Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin. in Journal of Functional Foods, 106, 105617.
https://doi.org/10.1016/j.jff.2023.105617

Joncić Savić K, Đokic L, Stanković N, Morić I, Pavlović B, Šenerović L, Aydogan C, Pavić A. Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin. in Journal of Functional Foods. 2023;106:105617.
doi:10.1016/j.jff.2023.105617 .

Joncić Savić, Katarina, Đokic, Lidija, Stanković, Nada, Morić, Ivana, Pavlović, Bojan, Šenerović, Lidija, Aydogan, Cem, Pavić, Aleksandar, "Maqui berry extract inhibits filamentation of Candidaalbicans and improves the antifungal efficacy of nystatin" in Journal of Functional Foods, 106 (2023):105617,
https://doi.org/10.1016/j.jff.2023.105617 . .

TY  - CONF
AU  - Atanasković, Marija
AU  - Morić, Ivana
AU  - Šenerović, Lidija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1905
AB  - About one in ten people contract a foodborne illness within a year. Children under the age of five are
the most affected, with 125,000 deaths each year. Many of the foodborne illness outbreaks can be
linked to the presence of biofilms in the food industry, and Salmonella enteritidis is an extremely
important foodborne pathogen that thrives in these conditions. It has been shown that biofilms can be
resistant to physical and chemical treatments used in cleaning and disinfection procedures in food
processing. The problem with using more aggressive disinfectants is that they often violate food safety
regulations. The use of enzymes which degrade biofilm matrix structural components should facilitate
current disinfection procedures and not compromise food safety. In this study, the anti-biofilm activity
of recombinantly expressed β-glucosidase B and its potential use as a protective agent to control
Salmonella biofilm formation is investigated. The putative target of this enzyme is cellulose, the
structural component of the Salmonella biofilm matrix. β-Glucosidase B deriving from the
environmental strain Microbacterium sp. BG28 was heterologously expressed in Escherichia coli and
successfully purified by affinity chromatography. The anti-biofilm activity of the enzyme was
evaluated in in vitro assays using various clinical isolates of S. enteritidis. The toxicity of the enzyme
was studied in Caenorhabditis elegans. β-glucosidase B effectively inhibited the formation of
Salmonella biofilms grown in a temperature range of 8°C to 37°C, achieving 50% inhibition at
concentrations of 100μg/ml. Biochemical characterization showed that the optimal pH activity of the
enzyme is between 6 and 7, with the highest activity observed at temperatures between 37°C and 47°C.
The absence of toxicity and other presented results indicate that beta-glucosidase B can be used in
biofilm control in the food industry.
PB  - Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Genetics & Applications
T1  - Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment
IS  - 2 (Special edition)
SP  - 129
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1905
ER  - 

@conference{
author = "Atanasković, Marija and Morić, Ivana and Šenerović, Lidija",
year = "2023",
abstract = "About one in ten people contract a foodborne illness within a year. Children under the age of five are
the most affected, with 125,000 deaths each year. Many of the foodborne illness outbreaks can be
linked to the presence of biofilms in the food industry, and Salmonella enteritidis is an extremely
important foodborne pathogen that thrives in these conditions. It has been shown that biofilms can be
resistant to physical and chemical treatments used in cleaning and disinfection procedures in food
processing. The problem with using more aggressive disinfectants is that they often violate food safety
regulations. The use of enzymes which degrade biofilm matrix structural components should facilitate
current disinfection procedures and not compromise food safety. In this study, the anti-biofilm activity
of recombinantly expressed β-glucosidase B and its potential use as a protective agent to control
Salmonella biofilm formation is investigated. The putative target of this enzyme is cellulose, the
structural component of the Salmonella biofilm matrix. β-Glucosidase B deriving from the
environmental strain Microbacterium sp. BG28 was heterologously expressed in Escherichia coli and
successfully purified by affinity chromatography. The anti-biofilm activity of the enzyme was
evaluated in in vitro assays using various clinical isolates of S. enteritidis. The toxicity of the enzyme
was studied in Caenorhabditis elegans. β-glucosidase B effectively inhibited the formation of
Salmonella biofilms grown in a temperature range of 8°C to 37°C, achieving 50% inhibition at
concentrations of 100μg/ml. Biochemical characterization showed that the optimal pH activity of the
enzyme is between 6 and 7, with the highest activity observed at temperatures between 37°C and 47°C.
The absence of toxicity and other presented results indicate that beta-glucosidase B can be used in
biofilm control in the food industry.",
publisher = "Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Genetics & Applications",
title = "Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment",
number = "2 (Special edition)",
pages = "129",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1905"
}

Atanasković, M., Morić, I.,& Šenerović, L.. (2023). Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment. in Genetics & Applications
Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 7(2 (Special edition)), 129.
https://hdl.handle.net/21.15107/rcub_imagine_1905

Atanasković M, Morić I, Šenerović L. Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment. in Genetics & Applications. 2023;7(2 (Special edition)):129.
https://hdl.handle.net/21.15107/rcub_imagine_1905 .

Atanasković, Marija, Morić, Ivana, Šenerović, Lidija, "Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment" in Genetics & Applications, 7, no. 2 (Special edition) (2023):129,
https://hdl.handle.net/21.15107/rcub_imagine_1905 .

TY  - JOUR
AU  - Kokot, Maja
AU  - Weiss, Matjaž
AU  - Zdovc, Irena
AU  - Šenerović, Lidija
AU  - Radakovic, Natasa
AU  - Anderluh, Marko
AU  - Minovski, Nikola
AU  - Hrast, Martina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0223523423000752
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1779
AB  - Novel bacterial topoisomerase inhibitors (NBTIs) are new promising antimicrobials for the treatment of multidrug-resistant bacterial infections. In recent years, many new NBTIs have been discovered, however most of them struggle with the same issue - the balance between antibacterial activity and hERG-related toxicity. We started a new campaign by optimizing the previous series of NBTIs, followed by the design and synthesis of a new, amide-containing focused NBTI library to reduce hERG inhibition and maintain antibacterial activity against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). This optimization strategy yielded the lead compound 12 that exhibits potent antibacterial activity against Gram-positive bacteria, reduced hERG inhibition, no cardiotoxicity in zebrafish model, and a favorable in vivo efficacy in a neutropenic murine thigh infection model of MRSA infection.
T2  - European Journal of Medicinal Chemistry
T2  - European Journal of Medicinal ChemistryEuropean Journal of Medicinal Chemistry
T1  - Amide containing NBTI antibacterials with reduced hERG inhibition, retained antimicrobial activity against gram-positive bacteria and in vivo efficacy
SP  - 115160
VL  - 250
DO  - 10.1016/j.ejmech.2023.115160
ER  - 

@article{
author = "Kokot, Maja and Weiss, Matjaž and Zdovc, Irena and Šenerović, Lidija and Radakovic, Natasa and Anderluh, Marko and Minovski, Nikola and Hrast, Martina",
year = "2023",
abstract = "Novel bacterial topoisomerase inhibitors (NBTIs) are new promising antimicrobials for the treatment of multidrug-resistant bacterial infections. In recent years, many new NBTIs have been discovered, however most of them struggle with the same issue - the balance between antibacterial activity and hERG-related toxicity. We started a new campaign by optimizing the previous series of NBTIs, followed by the design and synthesis of a new, amide-containing focused NBTI library to reduce hERG inhibition and maintain antibacterial activity against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). This optimization strategy yielded the lead compound 12 that exhibits potent antibacterial activity against Gram-positive bacteria, reduced hERG inhibition, no cardiotoxicity in zebrafish model, and a favorable in vivo efficacy in a neutropenic murine thigh infection model of MRSA infection.",
journal = "European Journal of Medicinal Chemistry, European Journal of Medicinal ChemistryEuropean Journal of Medicinal Chemistry",
title = "Amide containing NBTI antibacterials with reduced hERG inhibition, retained antimicrobial activity against gram-positive bacteria and in vivo efficacy",
pages = "115160",
volume = "250",
doi = "10.1016/j.ejmech.2023.115160"
}

Kokot, M., Weiss, M., Zdovc, I., Šenerović, L., Radakovic, N., Anderluh, M., Minovski, N.,& Hrast, M.. (2023). Amide containing NBTI antibacterials with reduced hERG inhibition, retained antimicrobial activity against gram-positive bacteria and in vivo efficacy. in European Journal of Medicinal Chemistry, 250, 115160.
https://doi.org/10.1016/j.ejmech.2023.115160

Kokot M, Weiss M, Zdovc I, Šenerović L, Radakovic N, Anderluh M, Minovski N, Hrast M. Amide containing NBTI antibacterials with reduced hERG inhibition, retained antimicrobial activity against gram-positive bacteria and in vivo efficacy. in European Journal of Medicinal Chemistry. 2023;250:115160.
doi:10.1016/j.ejmech.2023.115160 .

Kokot, Maja, Weiss, Matjaž, Zdovc, Irena, Šenerović, Lidija, Radakovic, Natasa, Anderluh, Marko, Minovski, Nikola, Hrast, Martina, "Amide containing NBTI antibacterials with reduced hERG inhibition, retained antimicrobial activity against gram-positive bacteria and in vivo efficacy" in European Journal of Medicinal Chemistry, 250 (2023):115160,
https://doi.org/10.1016/j.ejmech.2023.115160 . .

TY  - JOUR
AU  - Đokić, Lidija
AU  - Stanković, Nada
AU  - Galić, Ivana
AU  - Morić, Ivana
AU  - Radaković, Nataša
AU  - Segan, Sandra
AU  - Pavić, Aleksandar
AU  - Šenerović, Lidija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1596
AB  - Bacterial infections have become increasingly difficult to treat due to the occurrence of antibiotic-resistant strains. A promising strategy to increase the efficacy of therapy is to combine antibacterials with agents that decrease pathogen virulence via the modulation of the quorum sensing (QS). Lactonases inhibit acylated homoserine lactone (AHL)-mediated QS in Gram-negative bacteria, including the leading nosocomial pathogen Pseudomonas aeruginosa. Here we describe the characteristics of heterologously expressed YtnP lactonase from Bacillus paralicheniformis ZP1 (YtnP-ZP1) isolated from agricultural soil using the culture enrichment method. Purified YtnP-ZP1 hydrolyzed different AHLs with preference to substrates with long acyl residues as evaluated in assays with biosensors and HPLC. The enzyme showed good thermostability and activity in a wide temperature range. YtnP-ZP1 in 50 mu g mL(-1) concentration reduced the amount of P. aeruginosa-produced long-chain AHLs by 85%, while it hydrolyzed 50% of short-chain AHLs. Incubation of P. aeruginosa PAO1 with YtnP-ZP1 reduced its swarming motility and elastolytic activity without bactericidal effect. YtnP-ZP1 caused the inhibition of biofilm formation and disintegration of mature biofilms in P. aeruginosa PAO1 and multiresistant clinical strain BR5H that was visualized by crystal violet staining. The treatment with YtnP-ZP1 in concentrations higher than 25 mu g mL(-1) improved the survival of P. aeruginosa PAO1-infected zebrafish (Danio rerio), rescuing 80% of embryos, while in combination with tobramycin or gentamicin survival rate increased to 100%. The treatment of P. aeruginosa PAO1 biofilms on infected zebrafish tail wounds with 50 mu g mL(-1) YtnP-ZP1 and 2 x MIC tobramycin led to infection clearing in 2 days. The extensive toxicity studies proved YtnP-ZP1 was non-toxic to human cells and zebrafish. In conclusion, novel YtnP-ZP1 lactonase with its effective anti-virulence activity could be used to increase the efficacy of clinically approved antibiotics in clearing both systemic and biofilm-associated P. aeruginosa infections.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo
VL  - 13
DO  - 10.3389/fmicb.2022.906312
ER  - 

@article{
author = "Đokić, Lidija and Stanković, Nada and Galić, Ivana and Morić, Ivana and Radaković, Nataša and Segan, Sandra and Pavić, Aleksandar and Šenerović, Lidija",
year = "2022",
abstract = "Bacterial infections have become increasingly difficult to treat due to the occurrence of antibiotic-resistant strains. A promising strategy to increase the efficacy of therapy is to combine antibacterials with agents that decrease pathogen virulence via the modulation of the quorum sensing (QS). Lactonases inhibit acylated homoserine lactone (AHL)-mediated QS in Gram-negative bacteria, including the leading nosocomial pathogen Pseudomonas aeruginosa. Here we describe the characteristics of heterologously expressed YtnP lactonase from Bacillus paralicheniformis ZP1 (YtnP-ZP1) isolated from agricultural soil using the culture enrichment method. Purified YtnP-ZP1 hydrolyzed different AHLs with preference to substrates with long acyl residues as evaluated in assays with biosensors and HPLC. The enzyme showed good thermostability and activity in a wide temperature range. YtnP-ZP1 in 50 mu g mL(-1) concentration reduced the amount of P. aeruginosa-produced long-chain AHLs by 85%, while it hydrolyzed 50% of short-chain AHLs. Incubation of P. aeruginosa PAO1 with YtnP-ZP1 reduced its swarming motility and elastolytic activity without bactericidal effect. YtnP-ZP1 caused the inhibition of biofilm formation and disintegration of mature biofilms in P. aeruginosa PAO1 and multiresistant clinical strain BR5H that was visualized by crystal violet staining. The treatment with YtnP-ZP1 in concentrations higher than 25 mu g mL(-1) improved the survival of P. aeruginosa PAO1-infected zebrafish (Danio rerio), rescuing 80% of embryos, while in combination with tobramycin or gentamicin survival rate increased to 100%. The treatment of P. aeruginosa PAO1 biofilms on infected zebrafish tail wounds with 50 mu g mL(-1) YtnP-ZP1 and 2 x MIC tobramycin led to infection clearing in 2 days. The extensive toxicity studies proved YtnP-ZP1 was non-toxic to human cells and zebrafish. In conclusion, novel YtnP-ZP1 lactonase with its effective anti-virulence activity could be used to increase the efficacy of clinically approved antibiotics in clearing both systemic and biofilm-associated P. aeruginosa infections.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo",
volume = "13",
doi = "10.3389/fmicb.2022.906312"
}

Đokić, L., Stanković, N., Galić, I., Morić, I., Radaković, N., Segan, S., Pavić, A.,& Šenerović, L.. (2022). Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 13.
https://doi.org/10.3389/fmicb.2022.906312

Đokić L, Stanković N, Galić I, Morić I, Radaković N, Segan S, Pavić A, Šenerović L. Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo. in Frontiers in Microbiology. 2022;13.
doi:10.3389/fmicb.2022.906312 .

Đokić, Lidija, Stanković, Nada, Galić, Ivana, Morić, Ivana, Radaković, Nataša, Segan, Sandra, Pavić, Aleksandar, Šenerović, Lidija, "Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo" in Frontiers in Microbiology, 13 (2022),
https://doi.org/10.3389/fmicb.2022.906312 . .

TY  - CONF
AU  - Galić, Ivana
AU  - Šenerović, Lidija
AU  - Stanković, Nada
PY  - 2022
UR  - https://2022.eccmid.org/abstracts/posters
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2162
AB  - Soils contain microorganisms capable of causing diseases in humans, either
as opportunistic or as obligate pathogens. The application of herbicides
increases the prevalence of antibiotic resistance genes and mobile genetic
elements, which expands the competitive niche of pathogenic microbes. This
implies that herbicides could impose selective pressure driving the spread of
antibiotic resistance and therefore making heavily herbicide treated agricultural
soil a possible reservoir of multiresistant opportunistic human pathogens.
From the soil exposed to chlorinated aromatic herbicides S-metolachlor
and isoxaflutole, semi-persistent selective herbicides widely used in
European Union and Serbia we isolated bacteria able to grow on herbicide as
the sole carbon source. Bacterial isolates were assessed for various virulence
traits and resistance to antibiotics in order to identify the strains with high
pathogenic potential.
C3  - European Congress of Clinical Microbiology & Infectious Diseases
T1  - Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans
VL  - 32
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2162
ER  - 

@conference{
author = "Galić, Ivana and Šenerović, Lidija and Stanković, Nada",
year = "2022",
abstract = "Soils contain microorganisms capable of causing diseases in humans, either
as opportunistic or as obligate pathogens. The application of herbicides
increases the prevalence of antibiotic resistance genes and mobile genetic
elements, which expands the competitive niche of pathogenic microbes. This
implies that herbicides could impose selective pressure driving the spread of
antibiotic resistance and therefore making heavily herbicide treated agricultural
soil a possible reservoir of multiresistant opportunistic human pathogens.
From the soil exposed to chlorinated aromatic herbicides S-metolachlor
and isoxaflutole, semi-persistent selective herbicides widely used in
European Union and Serbia we isolated bacteria able to grow on herbicide as
the sole carbon source. Bacterial isolates were assessed for various virulence
traits and resistance to antibiotics in order to identify the strains with high
pathogenic potential.",
journal = "European Congress of Clinical Microbiology & Infectious Diseases",
title = "Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans",
volume = "32",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2162"
}

Galić, I., Šenerović, L.,& Stanković, N.. (2022). Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans. in European Congress of Clinical Microbiology & Infectious Diseases, 32.
https://hdl.handle.net/21.15107/rcub_imagine_2162

Galić I, Šenerović L, Stanković N. Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans. in European Congress of Clinical Microbiology & Infectious Diseases. 2022;32.
https://hdl.handle.net/21.15107/rcub_imagine_2162 .

Galić, Ivana, Šenerović, Lidija, Stanković, Nada, "Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans" in European Congress of Clinical Microbiology & Infectious Diseases, 32 (2022),
https://hdl.handle.net/21.15107/rcub_imagine_2162 .

TY  - JOUR
AU  - Zizović, Irena
AU  - Tyrka, Marcin
AU  - Matyja, Konrad
AU  - Morić, Ivana
AU  - Šenerović, Lidija
AU  - Trusek, Anna
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1496
AB  - This study investigates the modification of commercial cellulose acetate microfiltration membranes by supercritical solvent impregnation with thymol to provide them with antibacterial properties. The impregnation process was conducted in a batch mode, and the effect of pressure and processing time on thymol loading was followed. The impact of the modification on the membrane's microstructure was analyzed using scanning electron and ion-beam microscopy, and membranes' functionality was tested in a cross-flow filtration system. The antibiofilm properties of the obtained materials were studied against Staphyloccocus aureus and Pseudomonas aeruginosa, while membranes' blocking in contact with bacteria was examined for S. aureus and Escherichia coli. The results revealed a fast impregnation process with high thymol loadings achievable after just 0.5 h at 15 MPa and 20 MPa. The presence of 20% of thymol provided strong antibiofilm properties against the tested strains without affecting the membrane's functionality. The study showed that these strong antibacterial properties could be implemented to the commercial membranes' defined polymeric structure in a short and environmentally friendly process.
PB  - MDPI, Basel
T2  - Molecules
T1  - Functional Modification of Cellulose Acetate Microfiltration Membranes by Supercritical Solvent Impregnation
IS  - 2
VL  - 26
DO  - 10.3390/molecules26020411
ER  - 

@article{
author = "Zizović, Irena and Tyrka, Marcin and Matyja, Konrad and Morić, Ivana and Šenerović, Lidija and Trusek, Anna",
year = "2021",
abstract = "This study investigates the modification of commercial cellulose acetate microfiltration membranes by supercritical solvent impregnation with thymol to provide them with antibacterial properties. The impregnation process was conducted in a batch mode, and the effect of pressure and processing time on thymol loading was followed. The impact of the modification on the membrane's microstructure was analyzed using scanning electron and ion-beam microscopy, and membranes' functionality was tested in a cross-flow filtration system. The antibiofilm properties of the obtained materials were studied against Staphyloccocus aureus and Pseudomonas aeruginosa, while membranes' blocking in contact with bacteria was examined for S. aureus and Escherichia coli. The results revealed a fast impregnation process with high thymol loadings achievable after just 0.5 h at 15 MPa and 20 MPa. The presence of 20% of thymol provided strong antibiofilm properties against the tested strains without affecting the membrane's functionality. The study showed that these strong antibacterial properties could be implemented to the commercial membranes' defined polymeric structure in a short and environmentally friendly process.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Functional Modification of Cellulose Acetate Microfiltration Membranes by Supercritical Solvent Impregnation",
number = "2",
volume = "26",
doi = "10.3390/molecules26020411"
}

Zizović, I., Tyrka, M., Matyja, K., Morić, I., Šenerović, L.,& Trusek, A.. (2021). Functional Modification of Cellulose Acetate Microfiltration Membranes by Supercritical Solvent Impregnation. in Molecules
MDPI, Basel., 26(2).
https://doi.org/10.3390/molecules26020411

Zizović I, Tyrka M, Matyja K, Morić I, Šenerović L, Trusek A. Functional Modification of Cellulose Acetate Microfiltration Membranes by Supercritical Solvent Impregnation. in Molecules. 2021;26(2).
doi:10.3390/molecules26020411 .

Zizović, Irena, Tyrka, Marcin, Matyja, Konrad, Morić, Ivana, Šenerović, Lidija, Trusek, Anna, "Functional Modification of Cellulose Acetate Microfiltration Membranes by Supercritical Solvent Impregnation" in Molecules, 26, no. 2 (2021),
https://doi.org/10.3390/molecules26020411 . .

TY  - JOUR
AU  - Zizović, Irena
AU  - Trusek, Anna
AU  - Tyrka, Marcin
AU  - Morić, Ivana
AU  - Šenerović, Lidija
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1441
AB  - The study reports the application of supercritical solvent impregnation to deliver antibacterial substance thymol to the defined polymeric structure of commercial polyamide microfiltration membranes. The process performed at a temperature of 40 degrees C and pressures of 10, 15, and 20 MPa was characterized by the fast impregnation in the first 30 min with thymol loadings up to 29 wt%. The maximal achieved thymol loading in the process was around 42 wt%, regardless the pressure. The scanning electron and ion microscopy analyses revealed that the membranes' microstructure was preserved with a thymol content of 20 wt%, while a more prominent swelling effect was observed in samples with 35 wt% of thymol. The cross-flow filtration tests showed that the impregnation process did not harm the membranes' functionality in samples with 20 wt% of thymol. These membranes exhibited strong anti-biofilm activity, and were less prone to the blockage with bacteria in comparison to neat membranes.
PB  - Elsevier, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Functionalization of polyamide microfiltration membranes by supercritical solvent impregnation
VL  - 174
DO  - 10.1016/j.supflu.2021.105250
ER  - 

@article{
author = "Zizović, Irena and Trusek, Anna and Tyrka, Marcin and Morić, Ivana and Šenerović, Lidija",
year = "2021",
abstract = "The study reports the application of supercritical solvent impregnation to deliver antibacterial substance thymol to the defined polymeric structure of commercial polyamide microfiltration membranes. The process performed at a temperature of 40 degrees C and pressures of 10, 15, and 20 MPa was characterized by the fast impregnation in the first 30 min with thymol loadings up to 29 wt%. The maximal achieved thymol loading in the process was around 42 wt%, regardless the pressure. The scanning electron and ion microscopy analyses revealed that the membranes' microstructure was preserved with a thymol content of 20 wt%, while a more prominent swelling effect was observed in samples with 35 wt% of thymol. The cross-flow filtration tests showed that the impregnation process did not harm the membranes' functionality in samples with 20 wt% of thymol. These membranes exhibited strong anti-biofilm activity, and were less prone to the blockage with bacteria in comparison to neat membranes.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Functionalization of polyamide microfiltration membranes by supercritical solvent impregnation",
volume = "174",
doi = "10.1016/j.supflu.2021.105250"
}

Zizović, I., Trusek, A., Tyrka, M., Morić, I.,& Šenerović, L.. (2021). Functionalization of polyamide microfiltration membranes by supercritical solvent impregnation. in Journal of Supercritical Fluids
Elsevier, Amsterdam., 174.
https://doi.org/10.1016/j.supflu.2021.105250

Zizović I, Trusek A, Tyrka M, Morić I, Šenerović L. Functionalization of polyamide microfiltration membranes by supercritical solvent impregnation. in Journal of Supercritical Fluids. 2021;174.
doi:10.1016/j.supflu.2021.105250 .

Zizović, Irena, Trusek, Anna, Tyrka, Marcin, Morić, Ivana, Šenerović, Lidija, "Functionalization of polyamide microfiltration membranes by supercritical solvent impregnation" in Journal of Supercritical Fluids, 174 (2021),
https://doi.org/10.1016/j.supflu.2021.105250 . .

TY  - CHAP
AU  - Šenerović, Lidija
AU  - Morić, Ivana
AU  - Milivojević, Dušan
AU  - Opsenica, Dejan
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1639
AB  - The rapid emergence of antibiotic-resistant pathogens presents a global healthcare challenge. Bacteria control their virulence, motility, and biofilm formation, all of them being required for establishing pathogenicity, through a cell density-dependent communication system known as quorum sensing (QS). QS comprises production of extracellular signaling molecules, their detection, and population-wide response involving regulation of the virulence genes expression. Inhibition of QS affects virulence and reduces harmful effects to the host and as such presents a promising strategy to fight antibiotic-resistant infections. Multiresistant Pseudomonas aeruginosa belogns to the group of most critical pathogens for which the introduction of new therapeutics is imperative. In the search for novel therapeutics nature continues to be inexhaustible source of bioactive scaffolds, which provide the bases for structure-based rational drug design enabling further exploitation of diverse natural structures. This review describes bacterial QS systems, highlights strategies for their manipulation, overviews nature-inspired antivirulence molecules primarily against P. aeruginosa, and discusses their perspectives.
PB  - Academic Press
T2  - Biodiversity and Biomedicine
T2  - Biodiversity and Biomedicine
T1  - Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections
EP  - 523
SP  - 497
DO  - 10.1016/B978-0-12-819541-3.00025-6
ER  - 

@inbook{
author = "Šenerović, Lidija and Morić, Ivana and Milivojević, Dušan and Opsenica, Dejan",
year = "2020",
abstract = "The rapid emergence of antibiotic-resistant pathogens presents a global healthcare challenge. Bacteria control their virulence, motility, and biofilm formation, all of them being required for establishing pathogenicity, through a cell density-dependent communication system known as quorum sensing (QS). QS comprises production of extracellular signaling molecules, their detection, and population-wide response involving regulation of the virulence genes expression. Inhibition of QS affects virulence and reduces harmful effects to the host and as such presents a promising strategy to fight antibiotic-resistant infections. Multiresistant Pseudomonas aeruginosa belogns to the group of most critical pathogens for which the introduction of new therapeutics is imperative. In the search for novel therapeutics nature continues to be inexhaustible source of bioactive scaffolds, which provide the bases for structure-based rational drug design enabling further exploitation of diverse natural structures. This review describes bacterial QS systems, highlights strategies for their manipulation, overviews nature-inspired antivirulence molecules primarily against P. aeruginosa, and discusses their perspectives.",
publisher = "Academic Press",
journal = "Biodiversity and Biomedicine, Biodiversity and Biomedicine",
booktitle = "Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections",
pages = "523-497",
doi = "10.1016/B978-0-12-819541-3.00025-6"
}

Šenerović, L., Morić, I., Milivojević, D.,& Opsenica, D.. (2020). Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections. in Biodiversity and Biomedicine
Academic Press., 497-523.
https://doi.org/10.1016/B978-0-12-819541-3.00025-6

Šenerović L, Morić I, Milivojević D, Opsenica D. Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections. in Biodiversity and Biomedicine. 2020;:497-523.
doi:10.1016/B978-0-12-819541-3.00025-6 .

Šenerović, Lidija, Morić, Ivana, Milivojević, Dušan, Opsenica, Dejan, "Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections" in Biodiversity and Biomedicine (2020):497-523,
https://doi.org/10.1016/B978-0-12-819541-3.00025-6 . .

TY  - JOUR
AU  - Gajić, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Šenerović, Lidija
AU  - Morić, Ivana
AU  - Šavikin, Katarina
AU  - Menković, Nebojša
AU  - Pejnović, Nada
AU  - Stojanović, Ivana D.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1638
AB  - Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually exhibit immunomodulatory, anti-viral and anti-bacterial effects. We have previously shown that the CE used in this study activated macrophages and stimulated effector T cell differentiation in vitro. When applied orally to healthy mice, CE increased the proportion of CD11c+ dendritic cells in the gut-associated lymphoid tissue. CE-pretreated BALB/c mice readily eradicated orally ingested Listeria monocytogenes as evidenced by a slighter decrease in body weight and number of bacteria recovered from the spleen and reduced spleen size compared to the control infected mice. CE pretreatment in infected mice resulted in higher proportions of CD11b+ macrophages and CD8+ cytotoxic T cells both in the gut and the spleen. Phagocytosis, reactive oxygen species production and the proportions of activated CD86+ macrophages (CD11b+) and dendritic cells (CD11c+) was also enhanced in CE-pretreated infected mice. Further, the expression of inducible nitric oxide synthase and IL-6 was increased in CE-pretreated infected mice and the similar results were obtained in peritoneal macrophages in vitro. This effect of CE was associated with increased phosphorylation of IκB and Notch1 production. Finally, CE pretreatment elevated the proportion of perforin-producing cells in the spleen compared to control infected mice. This study demonstrates that prophylactic treatment with CE leads to more rapid eradication of bacterial infection with Listeria monocytogenes predominantly through increased activity of myeloid cells in the gut and in the spleen.
PB  - United Kingdom : Royal Society of Chemistry
T2  - Food and Function
T1  - Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice
EP  - 7803
IS  - 9
SP  - 7793
VL  - 11
DO  - 10.1039/D0FO00946F
ER  - 

@article{
author = "Gajić, Dragica and Saksida, Tamara and Koprivica, Ivan and Šenerović, Lidija and Morić, Ivana and Šavikin, Katarina and Menković, Nebojša and Pejnović, Nada and Stojanović, Ivana D.",
year = "2020",
abstract = "Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually exhibit immunomodulatory, anti-viral and anti-bacterial effects. We have previously shown that the CE used in this study activated macrophages and stimulated effector T cell differentiation in vitro. When applied orally to healthy mice, CE increased the proportion of CD11c+ dendritic cells in the gut-associated lymphoid tissue. CE-pretreated BALB/c mice readily eradicated orally ingested Listeria monocytogenes as evidenced by a slighter decrease in body weight and number of bacteria recovered from the spleen and reduced spleen size compared to the control infected mice. CE pretreatment in infected mice resulted in higher proportions of CD11b+ macrophages and CD8+ cytotoxic T cells both in the gut and the spleen. Phagocytosis, reactive oxygen species production and the proportions of activated CD86+ macrophages (CD11b+) and dendritic cells (CD11c+) was also enhanced in CE-pretreated infected mice. Further, the expression of inducible nitric oxide synthase and IL-6 was increased in CE-pretreated infected mice and the similar results were obtained in peritoneal macrophages in vitro. This effect of CE was associated with increased phosphorylation of IκB and Notch1 production. Finally, CE pretreatment elevated the proportion of perforin-producing cells in the spleen compared to control infected mice. This study demonstrates that prophylactic treatment with CE leads to more rapid eradication of bacterial infection with Listeria monocytogenes predominantly through increased activity of myeloid cells in the gut and in the spleen.",
publisher = "United Kingdom : Royal Society of Chemistry",
journal = "Food and Function",
title = "Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice",
pages = "7803-7793",
number = "9",
volume = "11",
doi = "10.1039/D0FO00946F"
}

Gajić, D., Saksida, T., Koprivica, I., Šenerović, L., Morić, I., Šavikin, K., Menković, N., Pejnović, N.,& Stojanović, I. D.. (2020). Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice. in Food and Function
United Kingdom : Royal Society of Chemistry., 11(9), 7793-7803.
https://doi.org/10.1039/D0FO00946F

Gajić D, Saksida T, Koprivica I, Šenerović L, Morić I, Šavikin K, Menković N, Pejnović N, Stojanović ID. Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice. in Food and Function. 2020;11(9):7793-7803.
doi:10.1039/D0FO00946F .

Gajić, Dragica, Saksida, Tamara, Koprivica, Ivan, Šenerović, Lidija, Morić, Ivana, Šavikin, Katarina, Menković, Nebojša, Pejnović, Nada, Stojanović, Ivana D., "Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice" in Food and Function, 11, no. 9 (2020):7793-7803,
https://doi.org/10.1039/D0FO00946F . .

TY  - JOUR
AU  - Jovanović, Marina
AU  - Morić, Ivana
AU  - Nikolić, Biljana
AU  - Pavić, Aleksandar
AU  - Svircev, Emilija
AU  - Šenerović, Lidija
AU  - Mitić-Culafić, Dragana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1320
AB  - Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.
PB  - MDPI, Basel
T2  - Molecules
T1  - Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts
IS  - 8
VL  - 25
DO  - 10.3390/molecules25081811
ER  - 

@article{
author = "Jovanović, Marina and Morić, Ivana and Nikolić, Biljana and Pavić, Aleksandar and Svircev, Emilija and Šenerović, Lidija and Mitić-Culafić, Dragana",
year = "2020",
abstract = "Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts",
number = "8",
volume = "25",
doi = "10.3390/molecules25081811"
}

Jovanović, M., Morić, I., Nikolić, B., Pavić, A., Svircev, E., Šenerović, L.,& Mitić-Culafić, D.. (2020). Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules
MDPI, Basel., 25(8).
https://doi.org/10.3390/molecules25081811

Jovanović M, Morić I, Nikolić B, Pavić A, Svircev E, Šenerović L, Mitić-Culafić D. Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules. 2020;25(8).
doi:10.3390/molecules25081811 .

Jovanović, Marina, Morić, Ivana, Nikolić, Biljana, Pavić, Aleksandar, Svircev, Emilija, Šenerović, Lidija, Mitić-Culafić, Dragana, "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts" in Molecules, 25, no. 8 (2020),
https://doi.org/10.3390/molecules25081811 . .

TY  - CHAP
AU  - Šenerović, Lidija
AU  - Opsenica, Dejan
AU  - Morić, Ivana
AU  - Aleksić, Ivana
AU  - Spasić, M.
AU  - Vasiljević, Branka
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1348
AB  - Infective diseases have become health threat of a global proportion due to appearance and spread of microorganisms resistant to majority of therapeutics currently used for their treatment. Therefore, there is a constant need for development of new antimicrobial agents, as well as novel therapeutic strategies. Quinolines and quinolones, isolated from plants, animals, and microorganisms, have demonstrated numerous biological activities such as antimicrobial, insecticidal, anti-inflammatory, antiplatelet, and antitumor. For more than two centuries quinoline/quinolone moiety has been used as a scaffold for drug development and even today it represents an inexhaustible inspiration for design and development of novel semi-synthetic or synthetic agents exhibiting broad spectrum of bioactivities. The structural diversity of synthetized compounds provides high and selective activity attained through different mechanisms of action, as well as low toxicity on human cells. This review describes quinoline and quinolone derivatives with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic activities with the focus on the last 10 years literature.
PB  - Springer
T2  - Biophysics of Infection
T1  - Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents
EP  - 69
SP  - 37
VL  - 1282
DO  - 10.1007/5584_2019_428
ER  - 

@inbook{
author = "Šenerović, Lidija and Opsenica, Dejan and Morić, Ivana and Aleksić, Ivana and Spasić, M. and Vasiljević, Branka",
year = "2020",
abstract = "Infective diseases have become health threat of a global proportion due to appearance and spread of microorganisms resistant to majority of therapeutics currently used for their treatment. Therefore, there is a constant need for development of new antimicrobial agents, as well as novel therapeutic strategies. Quinolines and quinolones, isolated from plants, animals, and microorganisms, have demonstrated numerous biological activities such as antimicrobial, insecticidal, anti-inflammatory, antiplatelet, and antitumor. For more than two centuries quinoline/quinolone moiety has been used as a scaffold for drug development and even today it represents an inexhaustible inspiration for design and development of novel semi-synthetic or synthetic agents exhibiting broad spectrum of bioactivities. The structural diversity of synthetized compounds provides high and selective activity attained through different mechanisms of action, as well as low toxicity on human cells. This review describes quinoline and quinolone derivatives with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic activities with the focus on the last 10 years literature.",
publisher = "Springer",
journal = "Biophysics of Infection",
booktitle = "Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents",
pages = "69-37",
volume = "1282",
doi = "10.1007/5584_2019_428"
}

Šenerović, L., Opsenica, D., Morić, I., Aleksić, I., Spasić, M.,& Vasiljević, B.. (2020). Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents. in Biophysics of Infection
Springer., 1282, 37-69.
https://doi.org/10.1007/5584_2019_428

Šenerović L, Opsenica D, Morić I, Aleksić I, Spasić M, Vasiljević B. Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents. in Biophysics of Infection. 2020;1282:37-69.
doi:10.1007/5584_2019_428 .

Šenerović, Lidija, Opsenica, Dejan, Morić, Ivana, Aleksić, Ivana, Spasić, M., Vasiljević, Branka, "Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents" in Biophysics of Infection, 1282 (2020):37-69,
https://doi.org/10.1007/5584_2019_428 . .

TY  - JOUR
AU  - Gajić, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Šenerović, Lidija
AU  - Morić, Ivana
AU  - Savikin, Katarina
AU  - Menković, Nebojša
AU  - Pejnović, Nada
AU  - Stojanović, Ivana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1366
AB  - Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually exhibit immunomodulatory, anti-viral and anti-bacterial effects. We have previously shown that the CE used in this study activated macrophages and stimulated effector T cell differentiationin vitro. When applied orally to healthy mice, CE increased the proportion of CD11c(+)dendritic cells in the gut-associated lymphoid tissue. CE-pretreated BALB/c mice readily eradicated orally ingestedListeria monocytogenesas evidenced by a slighter decrease in body weight and number of bacteria recovered from the spleen and reduced spleen size compared to the control infected mice. CE pretreatment in infected mice resulted in higher proportions of CD11b(+)macrophages and CD8(+)cytotoxic T cells both in the gut and the spleen. Phagocytosis, reactive oxygen species production and the proportions of activated CD86(+)macrophages (CD11b(+)) and dendritic cells (CD11c(+)) were also enhanced in CE-pretreated infected mice. Furthermore, the expression of inducible nitric oxide synthase and IL-6 was increased in CE-pretreated infected mice and similar results were obtained in peritoneal macrophagesin vitro. This effect of CE was associated with increased phosphorylation of I kappa B and Notch1 production. Finally, CE pretreatment elevated the proportion of perforin-producing cells in the spleen compared to control infected mice. This study demonstrates that prophylactic treatment with CE leads to more rapid eradication of bacterial infection withL. monocytogenespredominantly through increased activity of myeloid cells in the gut and in the spleen.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Immunomodulatory activity and protective effects of chokeberry fruit extract onListeria monocytogenesinfection in mice
EP  - 7803
IS  - 9
SP  - 7793
VL  - 11
DO  - 10.1039/d0fo00946f
ER  - 

@article{
author = "Gajić, Dragica and Saksida, Tamara and Koprivica, Ivan and Šenerović, Lidija and Morić, Ivana and Savikin, Katarina and Menković, Nebojša and Pejnović, Nada and Stojanović, Ivana",
year = "2020",
abstract = "Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually exhibit immunomodulatory, anti-viral and anti-bacterial effects. We have previously shown that the CE used in this study activated macrophages and stimulated effector T cell differentiationin vitro. When applied orally to healthy mice, CE increased the proportion of CD11c(+)dendritic cells in the gut-associated lymphoid tissue. CE-pretreated BALB/c mice readily eradicated orally ingestedListeria monocytogenesas evidenced by a slighter decrease in body weight and number of bacteria recovered from the spleen and reduced spleen size compared to the control infected mice. CE pretreatment in infected mice resulted in higher proportions of CD11b(+)macrophages and CD8(+)cytotoxic T cells both in the gut and the spleen. Phagocytosis, reactive oxygen species production and the proportions of activated CD86(+)macrophages (CD11b(+)) and dendritic cells (CD11c(+)) were also enhanced in CE-pretreated infected mice. Furthermore, the expression of inducible nitric oxide synthase and IL-6 was increased in CE-pretreated infected mice and similar results were obtained in peritoneal macrophagesin vitro. This effect of CE was associated with increased phosphorylation of I kappa B and Notch1 production. Finally, CE pretreatment elevated the proportion of perforin-producing cells in the spleen compared to control infected mice. This study demonstrates that prophylactic treatment with CE leads to more rapid eradication of bacterial infection withL. monocytogenespredominantly through increased activity of myeloid cells in the gut and in the spleen.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Immunomodulatory activity and protective effects of chokeberry fruit extract onListeria monocytogenesinfection in mice",
pages = "7803-7793",
number = "9",
volume = "11",
doi = "10.1039/d0fo00946f"
}

Gajić, D., Saksida, T., Koprivica, I., Šenerović, L., Morić, I., Savikin, K., Menković, N., Pejnović, N.,& Stojanović, I.. (2020). Immunomodulatory activity and protective effects of chokeberry fruit extract onListeria monocytogenesinfection in mice. in Food & Function
Royal Soc Chemistry, Cambridge., 11(9), 7793-7803.
https://doi.org/10.1039/d0fo00946f

Gajić D, Saksida T, Koprivica I, Šenerović L, Morić I, Savikin K, Menković N, Pejnović N, Stojanović I. Immunomodulatory activity and protective effects of chokeberry fruit extract onListeria monocytogenesinfection in mice. in Food & Function. 2020;11(9):7793-7803.
doi:10.1039/d0fo00946f .

Gajić, Dragica, Saksida, Tamara, Koprivica, Ivan, Šenerović, Lidija, Morić, Ivana, Savikin, Katarina, Menković, Nebojša, Pejnović, Nada, Stojanović, Ivana, "Immunomodulatory activity and protective effects of chokeberry fruit extract onListeria monocytogenesinfection in mice" in Food & Function, 11, no. 9 (2020):7793-7803,
https://doi.org/10.1039/d0fo00946f . .

TY  - JOUR
AU  - Malešević, Milka
AU  - Di Lorenzo, Flaviana
AU  - Filipić, Brankica
AU  - Stanisavljević, Nemanja
AU  - Novović, Katarina
AU  - Šenerović, Lidija
AU  - Polović, Natalija
AU  - Molinaro, Antonio
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1217
AB  - Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C-18-HSL). Commercial C-18-HSL (20 mu M) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones
VL  - 9
DO  - 10.1038/s41598-019-52955-3
ER  - 

@article{
author = "Malešević, Milka and Di Lorenzo, Flaviana and Filipić, Brankica and Stanisavljević, Nemanja and Novović, Katarina and Šenerović, Lidija and Polović, Natalija and Molinaro, Antonio and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C-18-HSL). Commercial C-18-HSL (20 mu M) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones",
volume = "9",
doi = "10.1038/s41598-019-52955-3"
}

Malešević, M., Di Lorenzo, F., Filipić, B., Stanisavljević, N., Novović, K., Šenerović, L., Polović, N., Molinaro, A., Kojić, M.,& Jovčić, B.. (2019). Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones. in Scientific Reports
Nature Publishing Group, London., 9.
https://doi.org/10.1038/s41598-019-52955-3

Malešević M, Di Lorenzo F, Filipić B, Stanisavljević N, Novović K, Šenerović L, Polović N, Molinaro A, Kojić M, Jovčić B. Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones. in Scientific Reports. 2019;9.
doi:10.1038/s41598-019-52955-3 .

Malešević, Milka, Di Lorenzo, Flaviana, Filipić, Brankica, Stanisavljević, Nemanja, Novović, Katarina, Šenerović, Lidija, Polović, Natalija, Molinaro, Antonio, Kojić, Milan, Jovčić, Branko, "Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones" in Scientific Reports, 9 (2019),
https://doi.org/10.1038/s41598-019-52955-3 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Jeremić, Jelena
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Segan, Sandra
AU  - Zlatović, Mario
AU  - Opsenica, Dejan M.
AU  - Šenerović, Lidija
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1225
AB  - Pseudomonas aeruginosa is a leading cause of nosocomial infections that are becoming increasingly difficult to treat due to the occurrence of antibiotic resistant strains. Since P. aeruginosa virulence is controlled through quorum sensing, small molecule treatments inhibiting quorum sensing signaling pathways provide a promising therapeutic option. Consequently, we synthesized a series of N-octaneamino-4-aminoquinoline derivatives to optimize this chemotype's antivirulence activity against P. aeruginosa via inhibition of pyocyanin production. The most potent derivative, which possesses a benzofuran substituent, provided effective inhibition of pyocyanin production (IC50 = 12 mu M), biofilm formation (BFIC50 = 50 mu M), and motility. Experimentally, the compound's activity is achieved through competitive inhibition of PqsR, and structure-activity data were rationalized using molecular docking studies.
PB  - Amer Chemical Soc, Washington
T2  - Acs Chemical Biology
T1  - N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa
EP  - 2809
IS  - 12
SP  - 2800
VL  - 14
DO  - 10.1021/acschembio.9b00682
ER  - 

@article{
author = "Aleksić, Ivana and Jeremić, Jelena and Milivojević, Dušan and Ilić-Tomić, Tatjana and Segan, Sandra and Zlatović, Mario and Opsenica, Dejan M. and Šenerović, Lidija",
year = "2019",
abstract = "Pseudomonas aeruginosa is a leading cause of nosocomial infections that are becoming increasingly difficult to treat due to the occurrence of antibiotic resistant strains. Since P. aeruginosa virulence is controlled through quorum sensing, small molecule treatments inhibiting quorum sensing signaling pathways provide a promising therapeutic option. Consequently, we synthesized a series of N-octaneamino-4-aminoquinoline derivatives to optimize this chemotype's antivirulence activity against P. aeruginosa via inhibition of pyocyanin production. The most potent derivative, which possesses a benzofuran substituent, provided effective inhibition of pyocyanin production (IC50 = 12 mu M), biofilm formation (BFIC50 = 50 mu M), and motility. Experimentally, the compound's activity is achieved through competitive inhibition of PqsR, and structure-activity data were rationalized using molecular docking studies.",
publisher = "Amer Chemical Soc, Washington",
journal = "Acs Chemical Biology",
title = "N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa",
pages = "2809-2800",
number = "12",
volume = "14",
doi = "10.1021/acschembio.9b00682"
}

Aleksić, I., Jeremić, J., Milivojević, D., Ilić-Tomić, T., Segan, S., Zlatović, M., Opsenica, D. M.,& Šenerović, L.. (2019). N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa. in Acs Chemical Biology
Amer Chemical Soc, Washington., 14(12), 2800-2809.
https://doi.org/10.1021/acschembio.9b00682

Aleksić I, Jeremić J, Milivojević D, Ilić-Tomić T, Segan S, Zlatović M, Opsenica DM, Šenerović L. N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa. in Acs Chemical Biology. 2019;14(12):2800-2809.
doi:10.1021/acschembio.9b00682 .

Aleksić, Ivana, Jeremić, Jelena, Milivojević, Dušan, Ilić-Tomić, Tatjana, Segan, Sandra, Zlatović, Mario, Opsenica, Dejan M., Šenerović, Lidija, "N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa" in Acs Chemical Biology, 14, no. 12 (2019):2800-2809,
https://doi.org/10.1021/acschembio.9b00682 . .

TY  - DATA
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Comić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojkovic-Opsenica, Dusanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1768
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005
VL  - 222
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1768
ER  - 

@misc{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Comić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojkovic-Opsenica, Dusanka and Šenerović, Lidija",
year = "2018",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005",
volume = "222",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1768"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D.,& Šenerović, L.. (2018). Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222.
https://hdl.handle.net/21.15107/rcub_imagine_1768

Aleksić I, Ristivojević P, Pavić A, Radojević I, Comić LR, Vasiljević B, Opsenica D, Milojkovic-Opsenica D, Šenerović L. Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005. in Journal of Ethnopharmacology. 2018;222.
https://hdl.handle.net/21.15107/rcub_imagine_1768 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Comić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojkovic-Opsenica, Dusanka, Šenerović, Lidija, "Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005" in Journal of Ethnopharmacology, 222 (2018),
https://hdl.handle.net/21.15107/rcub_imagine_1768 .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Comić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojkovic-Opsenica, Dusanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1762
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
EP  - 158
SP  - 148
VL  - 222
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Comić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojkovic-Opsenica, Dusanka and Šenerović, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
pages = "158-148",
volume = "222",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D.,& Šenerović, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojević P, Pavić A, Radojević I, Comić LR, Vasiljević B, Opsenica D, Milojkovic-Opsenica D, Šenerović L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Comić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojkovic-Opsenica, Dusanka, Šenerović, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .

TY  - JOUR
AU  - Zizović, Irena
AU  - Šenerović, Lidija
AU  - Morić, Ivana
AU  - Adamović, Tijana
AU  - Jovanović, Milena
AU  - Krusić, Melina Kalagasidis
AU  - Mišić, Dušan
AU  - Stojanović, Dusica
AU  - Milovanović, Stoja
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1627
AB  - This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus
EP  - 20
SP  - 11
VL  - 140
DO  - 10.1016/j.supflu.2018.05.025
ER  - 

@article{
author = "Zizović, Irena and Šenerović, Lidija and Morić, Ivana and Adamović, Tijana and Jovanović, Milena and Krusić, Melina Kalagasidis and Mišić, Dušan and Stojanović, Dusica and Milovanović, Stoja",
year = "2018",
abstract = "This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus",
pages = "20-11",
volume = "140",
doi = "10.1016/j.supflu.2018.05.025"
}

Zizović, I., Šenerović, L., Morić, I., Adamović, T., Jovanović, M., Krusić, M. K., Mišić, D., Stojanović, D.,& Milovanović, S.. (2018). Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 140, 11-20.
https://doi.org/10.1016/j.supflu.2018.05.025

Zizović I, Šenerović L, Morić I, Adamović T, Jovanović M, Krusić MK, Mišić D, Stojanović D, Milovanović S. Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids. 2018;140:11-20.
doi:10.1016/j.supflu.2018.05.025 .

Zizović, Irena, Šenerović, Lidija, Morić, Ivana, Adamović, Tijana, Jovanović, Milena, Krusić, Melina Kalagasidis, Mišić, Dušan, Stojanović, Dusica, Milovanović, Stoja, "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus" in Journal of Supercritical Fluids, 140 (2018):11-20,
https://doi.org/10.1016/j.supflu.2018.05.025 . .

TY  - JOUR
AU  - Milivojević, Dušan
AU  - Sumonja, Neven
AU  - Medić, Strahinja
AU  - Pavić, Aleksandar
AU  - Morić, Ivana
AU  - Vasiljević, Branka
AU  - Šenerović, Lidija
AU  - Nikodinović-Runić, Jasmina
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1117
AB  - Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability.
PB  - Oxford Univ Press, Oxford
T2  - Pathogens and Disease
T1  - Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans
IS  - 4
VL  - 76
DO  - 10.1093/femspd/fty041
ER  - 

@article{
author = "Milivojević, Dušan and Sumonja, Neven and Medić, Strahinja and Pavić, Aleksandar and Morić, Ivana and Vasiljević, Branka and Šenerović, Lidija and Nikodinović-Runić, Jasmina",
year = "2018",
abstract = "Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability.",
publisher = "Oxford Univ Press, Oxford",
journal = "Pathogens and Disease",
title = "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans",
number = "4",
volume = "76",
doi = "10.1093/femspd/fty041"
}

Milivojević, D., Sumonja, N., Medić, S., Pavić, A., Morić, I., Vasiljević, B., Šenerović, L.,& Nikodinović-Runić, J.. (2018). Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease
Oxford Univ Press, Oxford., 76(4).
https://doi.org/10.1093/femspd/fty041

Milivojević D, Sumonja N, Medić S, Pavić A, Morić I, Vasiljević B, Šenerović L, Nikodinović-Runić J. Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease. 2018;76(4).
doi:10.1093/femspd/fty041 .

Milivojević, Dušan, Sumonja, Neven, Medić, Strahinja, Pavić, Aleksandar, Morić, Ivana, Vasiljević, Branka, Šenerović, Lidija, Nikodinović-Runić, Jasmina, "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans" in Pathogens and Disease, 76, no. 4 (2018),
https://doi.org/10.1093/femspd/fty041 . .

TY  - JOUR
AU  - Zizović, Irena
AU  - Šenerović, Lidija
AU  - Morić, Ivana
AU  - Adamović, Tijana
AU  - Jovanović, Milena
AU  - Krusić, Melina Kalagasidis
AU  - Mišić, Dušan
AU  - Stojanović, Dusica
AU  - Milovanović, Stoja
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1143
AB  - This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus
EP  - 20
SP  - 11
VL  - 140
DO  - 10.1016/j.supflu.2018.05.025
ER  - 

@article{
author = "Zizović, Irena and Šenerović, Lidija and Morić, Ivana and Adamović, Tijana and Jovanović, Milena and Krusić, Melina Kalagasidis and Mišić, Dušan and Stojanović, Dusica and Milovanović, Stoja",
year = "2018",
abstract = "This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus",
pages = "20-11",
volume = "140",
doi = "10.1016/j.supflu.2018.05.025"
}

Zizović, I., Šenerović, L., Morić, I., Adamović, T., Jovanović, M., Krusić, M. K., Mišić, D., Stojanović, D.,& Milovanović, S.. (2018). Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 140, 11-20.
https://doi.org/10.1016/j.supflu.2018.05.025

Zizović I, Šenerović L, Morić I, Adamović T, Jovanović M, Krusić MK, Mišić D, Stojanović D, Milovanović S. Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids. 2018;140:11-20.
doi:10.1016/j.supflu.2018.05.025 .

Zizović, Irena, Šenerović, Lidija, Morić, Ivana, Adamović, Tijana, Jovanović, Milena, Krusić, Melina Kalagasidis, Mišić, Dušan, Stojanović, Dusica, Milovanović, Stoja, "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus" in Journal of Supercritical Fluids, 140 (2018):11-20,
https://doi.org/10.1016/j.supflu.2018.05.025 . .