TY  - JOUR
AU  - Đokić, Jelena
AU  - Tomić, Sergej
AU  - Marković, Milan
AU  - Milosavljević, Petar
AU  - Čolić, Miodrag
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/701
AB  - Immunoregulatory mechanisms within periapical lesions (PLs) are as of yet unexplored. Considering the crucial role of DCs in controlling the immune response within PLs, the immunomodulatory properties of mesenchymal stem cells (MSCs), and the colocalization of MSCs and DCs in situ, we wondered whether MSCs from PLs modulate the development and functions of DCs. Using a model of monocyte-derived DCs, we showed that PL-MSCs inhibited differentiation of DCs via soluble factors, of which IL-6 had a minor effect, but did not impair their subsequent maturation induced by pro-inflammatory cytokines. However, upon maturation such DCs favored the production of Th2/Th17 cytokines by allogenic CD4(+) lymphocytes in coculture, compared with mature DCs differentiated without PL-MSCs. PL-MSC-differentiated DCs, cultivated with pro-inflammatory cytokines and PL-MSCs, although phenotypically mature, exhibited poor allostimulatory activity, induced anergy, Th2 polarization, differentiation of suppressive CD4(+)CD25(high)CD39(+) Treg-cell subsets via IDO-1-, ILT-3-, and ILT-4-dependent mechanisms, and increased production of TGF- in the coculture. In contrast, DCs cultivated with PL-MSCs only during maturation stimulated proliferation and Th1 polarization of CD4(+) T cells in an IL-12-independent manner. In conclusion, PL-MSCs significantly modulate the development and functions of DCs, depending on the phase of DCs development during which the interaction occurs.
PB  - Wiley-Blackwell, Hoboken
T2  - European Journal of Immunology
T1  - Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells
EP  - 1872
IS  - 7
SP  - 1862
VL  - 43
DO  - 10.1002/eji.201243010
ER  - 

@article{
author = "Đokić, Jelena and Tomić, Sergej and Marković, Milan and Milosavljević, Petar and Čolić, Miodrag",
year = "2013",
abstract = "Immunoregulatory mechanisms within periapical lesions (PLs) are as of yet unexplored. Considering the crucial role of DCs in controlling the immune response within PLs, the immunomodulatory properties of mesenchymal stem cells (MSCs), and the colocalization of MSCs and DCs in situ, we wondered whether MSCs from PLs modulate the development and functions of DCs. Using a model of monocyte-derived DCs, we showed that PL-MSCs inhibited differentiation of DCs via soluble factors, of which IL-6 had a minor effect, but did not impair their subsequent maturation induced by pro-inflammatory cytokines. However, upon maturation such DCs favored the production of Th2/Th17 cytokines by allogenic CD4(+) lymphocytes in coculture, compared with mature DCs differentiated without PL-MSCs. PL-MSC-differentiated DCs, cultivated with pro-inflammatory cytokines and PL-MSCs, although phenotypically mature, exhibited poor allostimulatory activity, induced anergy, Th2 polarization, differentiation of suppressive CD4(+)CD25(high)CD39(+) Treg-cell subsets via IDO-1-, ILT-3-, and ILT-4-dependent mechanisms, and increased production of TGF- in the coculture. In contrast, DCs cultivated with PL-MSCs only during maturation stimulated proliferation and Th1 polarization of CD4(+) T cells in an IL-12-independent manner. In conclusion, PL-MSCs significantly modulate the development and functions of DCs, depending on the phase of DCs development during which the interaction occurs.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "European Journal of Immunology",
title = "Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells",
pages = "1872-1862",
number = "7",
volume = "43",
doi = "10.1002/eji.201243010"
}

Đokić, J., Tomić, S., Marković, M., Milosavljević, P.,& Čolić, M.. (2013). Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells. in European Journal of Immunology
Wiley-Blackwell, Hoboken., 43(7), 1862-1872.
https://doi.org/10.1002/eji.201243010

Đokić J, Tomić S, Marković M, Milosavljević P, Čolić M. Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells. in European Journal of Immunology. 2013;43(7):1862-1872.
doi:10.1002/eji.201243010 .

Đokić, Jelena, Tomić, Sergej, Marković, Milan, Milosavljević, Petar, Čolić, Miodrag, "Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells" in European Journal of Immunology, 43, no. 7 (2013):1862-1872,
https://doi.org/10.1002/eji.201243010 . .