TY  - JOUR
AU  - Rat, M.
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Radak, B.
AU  - Bokić, B.
AU  - Radović, S.
AU  - Anačkov, G.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/758
AB  - We report an efficient, simple and cost-effective protocol for the isolation of genomic DNA from Ornithogalum species. Our modification of the standard CTAB protocol includes two polyphenol adsorbents (insoluble PVPP and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and addition of phenol to remove proteins and other contaminants. DNA yield obtained with our protocol was 223 and 312 μg DNA g-1 of dry leaf tissue. The absorbance ratio 260/280 nm was 1.879 (O. refractum) and 1.753 (O. sibthorpii), and the absorbance ratio 260/230 nm was 1.779 (O. refractum) and 1.545 (O. sibthorpii), revealing lack of contamination. PCR amplifications of one nuclear marker (26S rDNA) indicated that this DNA isolation protocol may be used for Ornithogalum plants containing many interfering compounds for further analyses in population genetics and phylogeographic studies.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales)
EP  - 190
IS  - 1
SP  - 185
VL  - 38
UR  - https://hdl.handle.net/21.15107/rcub_imagine_758
ER  - 

@article{
author = "Rat, M. and Jovanović, Živko and Stanisavljević, Nemanja and Radak, B. and Bokić, B. and Radović, S. and Anačkov, G.",
year = "2014",
abstract = "We report an efficient, simple and cost-effective protocol for the isolation of genomic DNA from Ornithogalum species. Our modification of the standard CTAB protocol includes two polyphenol adsorbents (insoluble PVPP and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and addition of phenol to remove proteins and other contaminants. DNA yield obtained with our protocol was 223 and 312 μg DNA g-1 of dry leaf tissue. The absorbance ratio 260/280 nm was 1.879 (O. refractum) and 1.753 (O. sibthorpii), and the absorbance ratio 260/230 nm was 1.779 (O. refractum) and 1.545 (O. sibthorpii), revealing lack of contamination. PCR amplifications of one nuclear marker (26S rDNA) indicated that this DNA isolation protocol may be used for Ornithogalum plants containing many interfering compounds for further analyses in population genetics and phylogeographic studies.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales)",
pages = "190-185",
number = "1",
volume = "38",
url = "https://hdl.handle.net/21.15107/rcub_imagine_758"
}

Rat, M., Jovanović, Ž., Stanisavljević, N., Radak, B., Bokić, B., Radović, S.,& Anačkov, G.. (2014). A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales). in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 38(1), 185-190.
https://hdl.handle.net/21.15107/rcub_imagine_758

Rat M, Jovanović Ž, Stanisavljević N, Radak B, Bokić B, Radović S, Anačkov G. A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales). in Botanica Serbica. 2014;38(1):185-190.
https://hdl.handle.net/21.15107/rcub_imagine_758 .

Rat, M., Jovanović, Živko, Stanisavljević, Nemanja, Radak, B., Bokić, B., Radović, S., Anačkov, G., "A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales)" in Botanica Serbica, 38, no. 1 (2014):185-190,
https://hdl.handle.net/21.15107/rcub_imagine_758 .