TY  - JOUR
AU  - Divac Rankov, Aleksandra
AU  - Kušić-Tišma, Jelena
AU  - Ljujić, Mila
AU  - Nikolić, Aleksandra
AU  - Milosević, Katarina
AU  - Dautović, Gordana Vilotijevic
AU  - Radojković, Dragica
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1381
AB  - Background: High heterogeneity levels of cystic fibrosis transmembrane regulator (CFTR) are manifested in different populations. The aim of this study was to analyze comprehensively all mutations in the CFTR gene in Serbian patients with cystic fibrosis (CF) and to use the findings to propose a testing algorithm for the Serbian population. Materials and Methods: Cascade screening was employed to detect mutations in the CFTR gene of 90 patients suspected of having CF, using polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism or PCR-mediated site directed mutagenesis, Sanger sequencing, and/or next-generation sequencing. Results: This is the first report for the Serbian CF population where single nucleotide polymorphisms, small insertions and deletions, large genome rearrangements, and copy number variants were analyzed in detail. A high degree of heterogeneity within the CFTR was documented among our cohort of 90 patients. We identified 19 CF-causing mutations and 3 with varying consequences, including a previously unreported deletion of the entire exon 11. Conclusion: Considering the spectrum and frequency of mutations found, we recommend a multistep sequencing algorithm in combination with evaluation of large rearrangements for future analyses of the CFTR gene in the Serbian population.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Genetic Testing and Molecular Biomarkers
T1  - Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges
EP  - 216
IS  - 4
SP  - 212
VL  - 24
DO  - 10.1089/gtmb.2019.0171
ER  - 

@article{
author = "Divac Rankov, Aleksandra and Kušić-Tišma, Jelena and Ljujić, Mila and Nikolić, Aleksandra and Milosević, Katarina and Dautović, Gordana Vilotijevic and Radojković, Dragica",
year = "2020",
abstract = "Background: High heterogeneity levels of cystic fibrosis transmembrane regulator (CFTR) are manifested in different populations. The aim of this study was to analyze comprehensively all mutations in the CFTR gene in Serbian patients with cystic fibrosis (CF) and to use the findings to propose a testing algorithm for the Serbian population. Materials and Methods: Cascade screening was employed to detect mutations in the CFTR gene of 90 patients suspected of having CF, using polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism or PCR-mediated site directed mutagenesis, Sanger sequencing, and/or next-generation sequencing. Results: This is the first report for the Serbian CF population where single nucleotide polymorphisms, small insertions and deletions, large genome rearrangements, and copy number variants were analyzed in detail. A high degree of heterogeneity within the CFTR was documented among our cohort of 90 patients. We identified 19 CF-causing mutations and 3 with varying consequences, including a previously unreported deletion of the entire exon 11. Conclusion: Considering the spectrum and frequency of mutations found, we recommend a multistep sequencing algorithm in combination with evaluation of large rearrangements for future analyses of the CFTR gene in the Serbian population.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Genetic Testing and Molecular Biomarkers",
title = "Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges",
pages = "216-212",
number = "4",
volume = "24",
doi = "10.1089/gtmb.2019.0171"
}

Divac Rankov, A., Kušić-Tišma, J., Ljujić, M., Nikolić, A., Milosević, K., Dautović, G. V.,& Radojković, D.. (2020). Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges. in Genetic Testing and Molecular Biomarkers
Mary Ann Liebert, Inc, New Rochelle., 24(4), 212-216.
https://doi.org/10.1089/gtmb.2019.0171

Divac Rankov A, Kušić-Tišma J, Ljujić M, Nikolić A, Milosević K, Dautović GV, Radojković D. Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges. in Genetic Testing and Molecular Biomarkers. 2020;24(4):212-216.
doi:10.1089/gtmb.2019.0171 .

Divac Rankov, Aleksandra, Kušić-Tišma, Jelena, Ljujić, Mila, Nikolić, Aleksandra, Milosević, Katarina, Dautović, Gordana Vilotijevic, Radojković, Dragica, "Molecular Diagnostics of Cystic Fibrosis in Serbia: Our Approach to Meet the Diagnostic Challenges" in Genetic Testing and Molecular Biomarkers, 24, no. 4 (2020):212-216,
https://doi.org/10.1089/gtmb.2019.0171 . .

TY  - JOUR
AU  - Dragičević, Sandra
AU  - Kosnik, Mitja
AU  - Divac Rankov, Aleksandra
AU  - Rijavec, Matija
AU  - Milosević, Katarina
AU  - Korosec, Peter
AU  - Kavalar, Maja Skerbinjek
AU  - Nikolić, Aleksandra
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1180
AB  - Purpose The maintaining of asthma control is difficult due to high variability in response to therapy among patients. Since matrix metalloproteinase 9 (MMP9) is implicated in inflammation and remodeling of asthmatic airways, it could be associated with adequate response to asthma therapy. The aim of this study was to investigate whether variants in 3' end of the MMP9 gene are associated with clinical phenotype and responsiveness to treatment in children with asthma. Methods The study included 127 asthmatic children from Slovenia. Variants in the 3' end of the MMP9 gene were analyzed by direct DNA sequencing and the obtained results were correlated with clinical parameters. Results Two variants were detected, rs13925 and rs20544. For the variant rs20544, statistically significant difference in airway hyperresponsiveness (p = 0.011) and asthma control (p = 0.049) between genotypes was found. Patients with TT genotype had lower airway sensitivity, and after 12 months of treatment showed significant improvement in Asthma Control Test (ACT) scores compared to CC and CT genotype. For the variant rs13925, the association with lung function was observed. The carriers of A allele showed noticeable improvement of lung function after the first 6 months of treatment in comparison to the carriers of G allele (p = 0.046). Conclusion The main finding of our study is the association of MMP9 genotypes rs20544 TT and rs13925 AA and AG with better asthma control, and indirectly better response to treatment. Based on these results, MMP9 deserves further research as a potential predictive biomarker for asthma.
PB  - Springer, New York
T2  - Lung
T1  - The Variants in the 3 ' Untranslated Region of the Matrix Metalloproteinase 9 Gene as Modulators of Treatment Outcome in Children with Asthma
EP  - 303
IS  - 3
SP  - 297
VL  - 196
DO  - 10.1007/s00408-018-0113-y
ER  - 

@article{
author = "Dragičević, Sandra and Kosnik, Mitja and Divac Rankov, Aleksandra and Rijavec, Matija and Milosević, Katarina and Korosec, Peter and Kavalar, Maja Skerbinjek and Nikolić, Aleksandra",
year = "2018",
abstract = "Purpose The maintaining of asthma control is difficult due to high variability in response to therapy among patients. Since matrix metalloproteinase 9 (MMP9) is implicated in inflammation and remodeling of asthmatic airways, it could be associated with adequate response to asthma therapy. The aim of this study was to investigate whether variants in 3' end of the MMP9 gene are associated with clinical phenotype and responsiveness to treatment in children with asthma. Methods The study included 127 asthmatic children from Slovenia. Variants in the 3' end of the MMP9 gene were analyzed by direct DNA sequencing and the obtained results were correlated with clinical parameters. Results Two variants were detected, rs13925 and rs20544. For the variant rs20544, statistically significant difference in airway hyperresponsiveness (p = 0.011) and asthma control (p = 0.049) between genotypes was found. Patients with TT genotype had lower airway sensitivity, and after 12 months of treatment showed significant improvement in Asthma Control Test (ACT) scores compared to CC and CT genotype. For the variant rs13925, the association with lung function was observed. The carriers of A allele showed noticeable improvement of lung function after the first 6 months of treatment in comparison to the carriers of G allele (p = 0.046). Conclusion The main finding of our study is the association of MMP9 genotypes rs20544 TT and rs13925 AA and AG with better asthma control, and indirectly better response to treatment. Based on these results, MMP9 deserves further research as a potential predictive biomarker for asthma.",
publisher = "Springer, New York",
journal = "Lung",
title = "The Variants in the 3 ' Untranslated Region of the Matrix Metalloproteinase 9 Gene as Modulators of Treatment Outcome in Children with Asthma",
pages = "303-297",
number = "3",
volume = "196",
doi = "10.1007/s00408-018-0113-y"
}

Dragičević, S., Kosnik, M., Divac Rankov, A., Rijavec, M., Milosević, K., Korosec, P., Kavalar, M. S.,& Nikolić, A.. (2018). The Variants in the 3 ' Untranslated Region of the Matrix Metalloproteinase 9 Gene as Modulators of Treatment Outcome in Children with Asthma. in Lung
Springer, New York., 196(3), 297-303.
https://doi.org/10.1007/s00408-018-0113-y

Dragičević S, Kosnik M, Divac Rankov A, Rijavec M, Milosević K, Korosec P, Kavalar MS, Nikolić A. The Variants in the 3 ' Untranslated Region of the Matrix Metalloproteinase 9 Gene as Modulators of Treatment Outcome in Children with Asthma. in Lung. 2018;196(3):297-303.
doi:10.1007/s00408-018-0113-y .

Dragičević, Sandra, Kosnik, Mitja, Divac Rankov, Aleksandra, Rijavec, Matija, Milosević, Katarina, Korosec, Peter, Kavalar, Maja Skerbinjek, Nikolić, Aleksandra, "The Variants in the 3 ' Untranslated Region of the Matrix Metalloproteinase 9 Gene as Modulators of Treatment Outcome in Children with Asthma" in Lung, 196, no. 3 (2018):297-303,
https://doi.org/10.1007/s00408-018-0113-y . .

TY  - JOUR
AU  - Topić, Aleksandra
AU  - Francuski, Djordje
AU  - Nikolić, Aleksandra
AU  - Milosević, Katarina
AU  - Jovicić, Snežana
AU  - Marković, Bojan
AU  - Đukić, Mirjana
AU  - Radojković, Dragica
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1061
AB  - Introduction: The significance of oxidative stress in pathogenesis of childhood asthma was recognized, but its role in the clinical manifestations of disease is still unclear. Materials and Methods: The study was conducted in 96 asthmatic children. The urinary biomarker of oxidative stress, 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG/creatinine) was determined by using HPLC-MS/MS. ELISA was performed to measure myeloperoxidase (MPO) and Cu,Zn-superoxide dismutase (Cu, Zn-SOD) in serum. Results: Logistic regression analysis revealed that female gender, tobacco smoke exposure, and increased 8-oxodG/creatinine were associated with risk for intermittent asthma, while the positive allergy test and increased Cu, Zn-SOD were associated with eczema in asthmatic children. Higher MPO (p = 0.033), and percent of granulocytes (p = 0.030) were found in severe persistent asthma in comparison to intermittent or mild persistent asthma. Conclusion: The main findings that TSE-induced oxidative stress is a risk for intermittent asthma and eczema may be clinically significant for the disease prevention and therapeutic improvements.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Fetal and Pediatric Pathology
T1  - The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma
EP  - 303
IS  - 4
SP  - 294
VL  - 36
DO  - 10.1080/15513815.2017.1315199
ER  - 

@article{
author = "Topić, Aleksandra and Francuski, Djordje and Nikolić, Aleksandra and Milosević, Katarina and Jovicić, Snežana and Marković, Bojan and Đukić, Mirjana and Radojković, Dragica",
year = "2017",
abstract = "Introduction: The significance of oxidative stress in pathogenesis of childhood asthma was recognized, but its role in the clinical manifestations of disease is still unclear. Materials and Methods: The study was conducted in 96 asthmatic children. The urinary biomarker of oxidative stress, 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG/creatinine) was determined by using HPLC-MS/MS. ELISA was performed to measure myeloperoxidase (MPO) and Cu,Zn-superoxide dismutase (Cu, Zn-SOD) in serum. Results: Logistic regression analysis revealed that female gender, tobacco smoke exposure, and increased 8-oxodG/creatinine were associated with risk for intermittent asthma, while the positive allergy test and increased Cu, Zn-SOD were associated with eczema in asthmatic children. Higher MPO (p = 0.033), and percent of granulocytes (p = 0.030) were found in severe persistent asthma in comparison to intermittent or mild persistent asthma. Conclusion: The main findings that TSE-induced oxidative stress is a risk for intermittent asthma and eczema may be clinically significant for the disease prevention and therapeutic improvements.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Fetal and Pediatric Pathology",
title = "The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma",
pages = "303-294",
number = "4",
volume = "36",
doi = "10.1080/15513815.2017.1315199"
}

Topić, A., Francuski, D., Nikolić, A., Milosević, K., Jovicić, S., Marković, B., Đukić, M.,& Radojković, D.. (2017). The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma. in Fetal and Pediatric Pathology
Taylor & Francis Inc, Philadelphia., 36(4), 294-303.
https://doi.org/10.1080/15513815.2017.1315199

Topić A, Francuski D, Nikolić A, Milosević K, Jovicić S, Marković B, Đukić M, Radojković D. The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma. in Fetal and Pediatric Pathology. 2017;36(4):294-303.
doi:10.1080/15513815.2017.1315199 .

Topić, Aleksandra, Francuski, Djordje, Nikolić, Aleksandra, Milosević, Katarina, Jovicić, Snežana, Marković, Bojan, Đukić, Mirjana, Radojković, Dragica, "The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma" in Fetal and Pediatric Pathology, 36, no. 4 (2017):294-303,
https://doi.org/10.1080/15513815.2017.1315199 . .

TY  - JOUR
AU  - Dragičević, Sandra
AU  - Milosević, Katarina
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1018
AB  - Transforming growth factor beta 1 (TGFB1) is a multifunctional cytokine with a key role in asthma airway inflammation and remodeling. Since elevated levels of this cytokine in airways might be associated with response to asthma therapy, the aim of this study was to investigate whether the presence of the polymorphism C-509T in the promoter of the TGFB1 gene is associated with response to montelukast. A group of 102 asthmatic patients was genotyped for the presence of the C-509T polymorphism by DNA sequencing and subjected to induced sputum sampling. Cells from sputum samples and BEAS 2B cells were treated with montelukast and endogenous TGFB1 expression was measured by quantitative real-time polymerase chain reaction. The promoter activity was analyzed by luciferase assays in BEAS 2B cells transfected with constructs carrying variants -509C and -509T of the TGFB1 gene promoter. After treatment with montelukast, the decrease in TGFB1 gene expression was greater for the -509TT genotype (58.9%) than for the -509CC and -509CT genotypes (49.6% and 31.8%, respectively) (P=0.071). In BEAS 2B cells, expression of endogenous TGFB1 was reduced by about 27% after montelukast treatment, while luciferase activity of both promoter variants was increased after montelukast treatment (-509C allele: 48.3%, P=0.060; and -509T allele: 100.5%, P=0.062). A more intensive response was registered in the promoter containing the -509T allele, which had 135% higher activity than the -509C variant (P=0.035). This study showed that the presence of the -509T allele in the TGFB1 promoter might modulate effects of montelukast on TGFB1 gene expression, but future studies are necessary, taking into consideration other genetic and nongenetic factors. It is of potential importance for clinical management of asthma to clarify the influence of the C-509T polymorphism on the response to treatment with montelukast.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Pediatric Allergy Immunology and Pulmonology
T1  - Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast
EP  - 245
IS  - 4
SP  - 239
VL  - 30
DO  - 10.1089/ped.2017.0770
ER  - 

@article{
author = "Dragičević, Sandra and Milosević, Katarina and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2017",
abstract = "Transforming growth factor beta 1 (TGFB1) is a multifunctional cytokine with a key role in asthma airway inflammation and remodeling. Since elevated levels of this cytokine in airways might be associated with response to asthma therapy, the aim of this study was to investigate whether the presence of the polymorphism C-509T in the promoter of the TGFB1 gene is associated with response to montelukast. A group of 102 asthmatic patients was genotyped for the presence of the C-509T polymorphism by DNA sequencing and subjected to induced sputum sampling. Cells from sputum samples and BEAS 2B cells were treated with montelukast and endogenous TGFB1 expression was measured by quantitative real-time polymerase chain reaction. The promoter activity was analyzed by luciferase assays in BEAS 2B cells transfected with constructs carrying variants -509C and -509T of the TGFB1 gene promoter. After treatment with montelukast, the decrease in TGFB1 gene expression was greater for the -509TT genotype (58.9%) than for the -509CC and -509CT genotypes (49.6% and 31.8%, respectively) (P=0.071). In BEAS 2B cells, expression of endogenous TGFB1 was reduced by about 27% after montelukast treatment, while luciferase activity of both promoter variants was increased after montelukast treatment (-509C allele: 48.3%, P=0.060; and -509T allele: 100.5%, P=0.062). A more intensive response was registered in the promoter containing the -509T allele, which had 135% higher activity than the -509C variant (P=0.035). This study showed that the presence of the -509T allele in the TGFB1 promoter might modulate effects of montelukast on TGFB1 gene expression, but future studies are necessary, taking into consideration other genetic and nongenetic factors. It is of potential importance for clinical management of asthma to clarify the influence of the C-509T polymorphism on the response to treatment with montelukast.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Pediatric Allergy Immunology and Pulmonology",
title = "Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast",
pages = "245-239",
number = "4",
volume = "30",
doi = "10.1089/ped.2017.0770"
}

Dragičević, S., Milosević, K., Nestorović, B.,& Nikolić, A.. (2017). Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast. in Pediatric Allergy Immunology and Pulmonology
Mary Ann Liebert, Inc, New Rochelle., 30(4), 239-245.
https://doi.org/10.1089/ped.2017.0770

Dragičević S, Milosević K, Nestorović B, Nikolić A. Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast. in Pediatric Allergy Immunology and Pulmonology. 2017;30(4):239-245.
doi:10.1089/ped.2017.0770 .

Dragičević, Sandra, Milosević, Katarina, Nestorović, Branimir, Nikolić, Aleksandra, "Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast" in Pediatric Allergy Immunology and Pulmonology, 30, no. 4 (2017):239-245,
https://doi.org/10.1089/ped.2017.0770 . .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Radlović, Nedeljko
AU  - Dinić, Jelena
AU  - Milosević, Katarina
AU  - Radojković, Dragica
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/769
AB  - We present a case of a 19-year old male with uncommon initial clinical cystic fibrosis (CF) presentation and a rare CFTR genotype, homozygote for c.1393-1G gt A mutation (legacy name 1525-1G gt A).
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Cystic Fibrosis
T1  - Clinical presentation of mild cystic fibrosis in a Serbian patient homozygous for the CFTR mutation c.1393-1G  gt  A
EP  - 113
IS  - 1
SP  - 111
VL  - 13
DO  - 10.1016/j.jcf.2013.07.001
ER  - 

@article{
author = "Nikolić, Aleksandra and Radlović, Nedeljko and Dinić, Jelena and Milosević, Katarina and Radojković, Dragica",
year = "2014",
abstract = "We present a case of a 19-year old male with uncommon initial clinical cystic fibrosis (CF) presentation and a rare CFTR genotype, homozygote for c.1393-1G gt A mutation (legacy name 1525-1G gt A).",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Cystic Fibrosis",
title = "Clinical presentation of mild cystic fibrosis in a Serbian patient homozygous for the CFTR mutation c.1393-1G  gt  A",
pages = "113-111",
number = "1",
volume = "13",
doi = "10.1016/j.jcf.2013.07.001"
}

Nikolić, A., Radlović, N., Dinić, J., Milosević, K.,& Radojković, D.. (2014). Clinical presentation of mild cystic fibrosis in a Serbian patient homozygous for the CFTR mutation c.1393-1G  gt  A. in Journal of Cystic Fibrosis
Elsevier Science Bv, Amsterdam., 13(1), 111-113.
https://doi.org/10.1016/j.jcf.2013.07.001

Nikolić A, Radlović N, Dinić J, Milosević K, Radojković D. Clinical presentation of mild cystic fibrosis in a Serbian patient homozygous for the CFTR mutation c.1393-1G  gt  A. in Journal of Cystic Fibrosis. 2014;13(1):111-113.
doi:10.1016/j.jcf.2013.07.001 .

Nikolić, Aleksandra, Radlović, Nedeljko, Dinić, Jelena, Milosević, Katarina, Radojković, Dragica, "Clinical presentation of mild cystic fibrosis in a Serbian patient homozygous for the CFTR mutation c.1393-1G  gt  A" in Journal of Cystic Fibrosis, 13, no. 1 (2014):111-113,
https://doi.org/10.1016/j.jcf.2013.07.001 . .

TY  - JOUR
AU  - Rsovac, Snežana
AU  - Milosević, Katarina
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/786
AB  - Background. Mechanical ventilation is a frequently applied therapy in critically ill children and can be lifesaving in many cases. Clinical use of this technique has well documented benefits, but can be associated with different complications and adverse physiologic effects. Objectives. The aim of this study was to investigate the complications and clinical outcome of mechanical ventilation in Serbian pediatric patients. Material and Methods. The study encompassed 42 children with respiratory insufficiency that underwent mechanical ventilation during hospitalization over a period of 12 consecutive months. The influence of clinical and mechanical parameters on the occurrence of complications and clinical outcome were analyzed. Results. The patients were ventilated for a total of 432 days and a total of 61 complications were observed in 42 patients (97 complications per 1000 ventilation days). The most common complications associated with mechanical ventilation in Serbian pediatric patients with respiratory insufficiency were cardiovascular insufficiency (52.4%) and multiple organ failure (35.7%). High values of applied PIP ( gt 26 cm H2O), PEEP ( gt 6 cm H2O) and Tv ( gt 6 mL/kg) were associated with the occurrence of complications and negative clinical outcome. Conclusions. Complications of mechanical ventilation in the pediatric population occur frequently, but lower volumes/pressures of ventilation contribute to a decrease in incidence. Further studies are needed to examine associated risk factors and strategies to reduce the occurrence of complications and improve clinical outcome.
PB  - Wroclaw Medical Univ, Wroclaw
T2  - Advances in Clinical and Experimental Medicine
T1  - Complications of Mechanical Ventilation in Pediatric Patients in Serbia
EP  - 61
IS  - 1
SP  - 57
VL  - 23
DO  - 10.17219/acem/37022
ER  - 

@article{
author = "Rsovac, Snežana and Milosević, Katarina and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2014",
abstract = "Background. Mechanical ventilation is a frequently applied therapy in critically ill children and can be lifesaving in many cases. Clinical use of this technique has well documented benefits, but can be associated with different complications and adverse physiologic effects. Objectives. The aim of this study was to investigate the complications and clinical outcome of mechanical ventilation in Serbian pediatric patients. Material and Methods. The study encompassed 42 children with respiratory insufficiency that underwent mechanical ventilation during hospitalization over a period of 12 consecutive months. The influence of clinical and mechanical parameters on the occurrence of complications and clinical outcome were analyzed. Results. The patients were ventilated for a total of 432 days and a total of 61 complications were observed in 42 patients (97 complications per 1000 ventilation days). The most common complications associated with mechanical ventilation in Serbian pediatric patients with respiratory insufficiency were cardiovascular insufficiency (52.4%) and multiple organ failure (35.7%). High values of applied PIP ( gt 26 cm H2O), PEEP ( gt 6 cm H2O) and Tv ( gt 6 mL/kg) were associated with the occurrence of complications and negative clinical outcome. Conclusions. Complications of mechanical ventilation in the pediatric population occur frequently, but lower volumes/pressures of ventilation contribute to a decrease in incidence. Further studies are needed to examine associated risk factors and strategies to reduce the occurrence of complications and improve clinical outcome.",
publisher = "Wroclaw Medical Univ, Wroclaw",
journal = "Advances in Clinical and Experimental Medicine",
title = "Complications of Mechanical Ventilation in Pediatric Patients in Serbia",
pages = "61-57",
number = "1",
volume = "23",
doi = "10.17219/acem/37022"
}

Rsovac, S., Milosević, K., Nestorović, B.,& Nikolić, A.. (2014). Complications of Mechanical Ventilation in Pediatric Patients in Serbia. in Advances in Clinical and Experimental Medicine
Wroclaw Medical Univ, Wroclaw., 23(1), 57-61.
https://doi.org/10.17219/acem/37022

Rsovac S, Milosević K, Nestorović B, Nikolić A. Complications of Mechanical Ventilation in Pediatric Patients in Serbia. in Advances in Clinical and Experimental Medicine. 2014;23(1):57-61.
doi:10.17219/acem/37022 .

Rsovac, Snežana, Milosević, Katarina, Nestorović, Branimir, Nikolić, Aleksandra, "Complications of Mechanical Ventilation in Pediatric Patients in Serbia" in Advances in Clinical and Experimental Medicine, 23, no. 1 (2014):57-61,
https://doi.org/10.17219/acem/37022 . .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Milosević, Katarina
AU  - Bošković, Srđan
AU  - Nestorović, Branimir
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/709
AB  - The aim of this study was to investigate polymorphisms in the promoter region of the neutrophil elastase (ELANE) gene as potential modulators of the therapeutic response in children with idiopathic bronchiectasis. The study included 48 children between 5 and 17 years old who were diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of the thorax. In all patients therapy included administration of antibiotics, anti-inflammatory drugs, expectorants, and postural drainage. Response to therapy was evaluated by the change in FeNO levels before and after administration of therapy. The ELANE promoter region polymorphisms were analyzed by PCR-direct DNA sequencing. According to the predicted activity of ELANE genotypes, subjects were divided into two groups: low/intermediate activity (n = 18) and high activity (n = 30). Subjects in the group with high-activity genotype had higher initial FeNO levels and this difference was statistically significant (t = 2.906; p = 0.006). The difference between FeNO levels before and after therapy was also statistically significantly higher in children with high-activity genotype (t = 3.329; p = 0.002). Statistically significant correlation was observed between the change in FeNO levels and ELANE genotypes (r = 0.350; p = 0.015). Children with high-activity genotype had higher initial FeNO levels and showed better response to therapy than children with low/intermediate-activity genotypes.
PB  - Springer, New York
T2  - Lung
T1  - Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?
EP  - 599
IS  - 4
SP  - 595
VL  - 192
DO  - 10.1007/s00408-014-9596-3
ER  - 

@article{
author = "Nikolić, Aleksandra and Milosević, Katarina and Bošković, Srđan and Nestorović, Branimir",
year = "2014",
abstract = "The aim of this study was to investigate polymorphisms in the promoter region of the neutrophil elastase (ELANE) gene as potential modulators of the therapeutic response in children with idiopathic bronchiectasis. The study included 48 children between 5 and 17 years old who were diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of the thorax. In all patients therapy included administration of antibiotics, anti-inflammatory drugs, expectorants, and postural drainage. Response to therapy was evaluated by the change in FeNO levels before and after administration of therapy. The ELANE promoter region polymorphisms were analyzed by PCR-direct DNA sequencing. According to the predicted activity of ELANE genotypes, subjects were divided into two groups: low/intermediate activity (n = 18) and high activity (n = 30). Subjects in the group with high-activity genotype had higher initial FeNO levels and this difference was statistically significant (t = 2.906; p = 0.006). The difference between FeNO levels before and after therapy was also statistically significantly higher in children with high-activity genotype (t = 3.329; p = 0.002). Statistically significant correlation was observed between the change in FeNO levels and ELANE genotypes (r = 0.350; p = 0.015). Children with high-activity genotype had higher initial FeNO levels and showed better response to therapy than children with low/intermediate-activity genotypes.",
publisher = "Springer, New York",
journal = "Lung",
title = "Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?",
pages = "599-595",
number = "4",
volume = "192",
doi = "10.1007/s00408-014-9596-3"
}

Nikolić, A., Milosević, K., Bošković, S.,& Nestorović, B.. (2014). Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?. in Lung
Springer, New York., 192(4), 595-599.
https://doi.org/10.1007/s00408-014-9596-3

Nikolić A, Milosević K, Bošković S, Nestorović B. Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?. in Lung. 2014;192(4):595-599.
doi:10.1007/s00408-014-9596-3 .

Nikolić, Aleksandra, Milosević, Katarina, Bošković, Srđan, Nestorović, Branimir, "Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?" in Lung, 192, no. 4 (2014):595-599,
https://doi.org/10.1007/s00408-014-9596-3 . .

TY  - JOUR
AU  - Nestorović, Branimir
AU  - Milosević, Katarina
AU  - Rsovac, Snežana
AU  - Nikolić, Aleksandra
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/636
AB  - A 36-year-old woman was stung in the right wrist by a bee, suffered typical anaphylaxis, and was transferred to a local hospital. After a few hours, which corresponded to late-onset reaction, she developed shortness of breath and weakness and was transferred to the emergency department, where the diagnosis of anaphylaxis was confirmed. Serum complement levels, components C3 and C4, were undetectable. Flexible bronchoscopy excluded lung hemorrhage. She was on the ventilator for 4 days and was fully awake during that time. After the treatment, her improvement was rapid, and she fully recovered. Three weeks after she had been stung by a bee, skin prick tests to bee, wasp, and yellow jacket venom were done, and all tests came back negative as well as IgE and IgG4 antibodies to the same venoms. Coagulation factors and the complement, including C1q inhibitor, were normal. Occurrence of complement activation and consumption could point to the immune complexes as basis of pathophysiological mechanism. It remains unclear why such a reaction would involve only the right lung and why no detectable immune complexes were discovered. The clinical picture in the presented case resembled acute respiratory distress syndrome, but the exact nature of lung consolidation remains puzzling. The most likely explanation is a very rare case of airway obstruction. To our knowledge, this is the only reported case of anaphylaxis associated with undetectable serum complement levels. The potential role and diagnostic significance of hypocomplementemia in cases of anaphylaxis should be further investigated.
PB  - W B Saunders Co-Elsevier Inc, Philadelphia
T2  - American Journal of Emergency Medicine
T1  - Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female
IS  - 11
VL  - 31
DO  - 10.1016/j.ajem.2013.06.025
ER  - 

@article{
author = "Nestorović, Branimir and Milosević, Katarina and Rsovac, Snežana and Nikolić, Aleksandra",
year = "2013",
abstract = "A 36-year-old woman was stung in the right wrist by a bee, suffered typical anaphylaxis, and was transferred to a local hospital. After a few hours, which corresponded to late-onset reaction, she developed shortness of breath and weakness and was transferred to the emergency department, where the diagnosis of anaphylaxis was confirmed. Serum complement levels, components C3 and C4, were undetectable. Flexible bronchoscopy excluded lung hemorrhage. She was on the ventilator for 4 days and was fully awake during that time. After the treatment, her improvement was rapid, and she fully recovered. Three weeks after she had been stung by a bee, skin prick tests to bee, wasp, and yellow jacket venom were done, and all tests came back negative as well as IgE and IgG4 antibodies to the same venoms. Coagulation factors and the complement, including C1q inhibitor, were normal. Occurrence of complement activation and consumption could point to the immune complexes as basis of pathophysiological mechanism. It remains unclear why such a reaction would involve only the right lung and why no detectable immune complexes were discovered. The clinical picture in the presented case resembled acute respiratory distress syndrome, but the exact nature of lung consolidation remains puzzling. The most likely explanation is a very rare case of airway obstruction. To our knowledge, this is the only reported case of anaphylaxis associated with undetectable serum complement levels. The potential role and diagnostic significance of hypocomplementemia in cases of anaphylaxis should be further investigated.",
publisher = "W B Saunders Co-Elsevier Inc, Philadelphia",
journal = "American Journal of Emergency Medicine",
title = "Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female",
number = "11",
volume = "31",
doi = "10.1016/j.ajem.2013.06.025"
}

Nestorović, B., Milosević, K., Rsovac, S.,& Nikolić, A.. (2013). Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female. in American Journal of Emergency Medicine
W B Saunders Co-Elsevier Inc, Philadelphia., 31(11).
https://doi.org/10.1016/j.ajem.2013.06.025

Nestorović B, Milosević K, Rsovac S, Nikolić A. Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female. in American Journal of Emergency Medicine. 2013;31(11).
doi:10.1016/j.ajem.2013.06.025 .

Nestorović, Branimir, Milosević, Katarina, Rsovac, Snežana, Nikolić, Aleksandra, "Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female" in American Journal of Emergency Medicine, 31, no. 11 (2013),
https://doi.org/10.1016/j.ajem.2013.06.025 . .

TY  - JOUR
AU  - Milosević, Katarina
AU  - Nikolić, Aleksandra
AU  - Divac Rankov, Aleksandra
AU  - Ljujić, Mila
AU  - Nestorović, Branimir
AU  - Radojković, Dragica
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/652
AB  - This study has investigated a potential role of common Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variants in the etiology of noncystic fibrosis bronchiectasis in Serbian children. The study has encompassed 48 patients (19 male and 29 female, aged between 5 and 18 years, median age 10.6 +/- 3.3), diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of thorax and pathologic examination of lobectomy materials. The CFTR gene analysis was performed on genomic DNA extracted from peripheral blood samples of patients by polymerase chain reaction (PCR)-Mediated Site-Directed Mutagenesis method, Denaturing Gradient Gel Electrophoresis method, and DNA sequencing. Mutation c.1521_1523delCTT (F508del) was detected with an allelic frequency of 1.0%, and c.224G gt A (R75Q) variant. Carriers of c.1210-12T[5] (IVS8-5T) allele were significantly more common than in the general population (10.4% vs. 5.0%, P = 0.0302). The frequency of homozygotes for Met 470 allele was higher in patients than in the general population (33% vs. 20%), while heterozygotes for p. Met470Val were less frequent (31% vs. 50%), and this difference was statistically significant (P = 0.0222). The results obtained in this study indicate involvement of 2 common CFTR variants, c. 1210-12T[5] and c. 1408A, in idiopathic bronchiectasis in children, but this observation should be further confirmed by more extensive analysis of the CFTR gene in a larger group of patients.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Pediatric Allergy Immunology and Pulmonology
T1  - Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children
EP  - 98
IS  - 2
SP  - 93
VL  - 26
DO  - 10.1089/ped.2013.0238
ER  - 

@article{
author = "Milosević, Katarina and Nikolić, Aleksandra and Divac Rankov, Aleksandra and Ljujić, Mila and Nestorović, Branimir and Radojković, Dragica",
year = "2013",
abstract = "This study has investigated a potential role of common Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variants in the etiology of noncystic fibrosis bronchiectasis in Serbian children. The study has encompassed 48 patients (19 male and 29 female, aged between 5 and 18 years, median age 10.6 +/- 3.3), diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of thorax and pathologic examination of lobectomy materials. The CFTR gene analysis was performed on genomic DNA extracted from peripheral blood samples of patients by polymerase chain reaction (PCR)-Mediated Site-Directed Mutagenesis method, Denaturing Gradient Gel Electrophoresis method, and DNA sequencing. Mutation c.1521_1523delCTT (F508del) was detected with an allelic frequency of 1.0%, and c.224G gt A (R75Q) variant. Carriers of c.1210-12T[5] (IVS8-5T) allele were significantly more common than in the general population (10.4% vs. 5.0%, P = 0.0302). The frequency of homozygotes for Met 470 allele was higher in patients than in the general population (33% vs. 20%), while heterozygotes for p. Met470Val were less frequent (31% vs. 50%), and this difference was statistically significant (P = 0.0222). The results obtained in this study indicate involvement of 2 common CFTR variants, c. 1210-12T[5] and c. 1408A, in idiopathic bronchiectasis in children, but this observation should be further confirmed by more extensive analysis of the CFTR gene in a larger group of patients.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Pediatric Allergy Immunology and Pulmonology",
title = "Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children",
pages = "98-93",
number = "2",
volume = "26",
doi = "10.1089/ped.2013.0238"
}

Milosević, K., Nikolić, A., Divac Rankov, A., Ljujić, M., Nestorović, B.,& Radojković, D.. (2013). Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children. in Pediatric Allergy Immunology and Pulmonology
Mary Ann Liebert, Inc, New Rochelle., 26(2), 93-98.
https://doi.org/10.1089/ped.2013.0238

Milosević K, Nikolić A, Divac Rankov A, Ljujić M, Nestorović B, Radojković D. Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children. in Pediatric Allergy Immunology and Pulmonology. 2013;26(2):93-98.
doi:10.1089/ped.2013.0238 .

Milosević, Katarina, Nikolić, Aleksandra, Divac Rankov, Aleksandra, Ljujić, Mila, Nestorović, Branimir, Radojković, Dragica, "Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children" in Pediatric Allergy Immunology and Pulmonology, 26, no. 2 (2013):93-98,
https://doi.org/10.1089/ped.2013.0238 . .

TY  - CONF
AU  - Milosević, Katarina
AU  - Bošković, Srđan
AU  - Ljujić, Mila
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/675
PB  - European Respiratory Soc Journals Ltd, Sheffield
C3  - European Respiratory Journal
T1  - Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_imagine_675
ER  - 

@conference{
author = "Milosević, Katarina and Bošković, Srđan and Ljujić, Mila and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2013",
publisher = "European Respiratory Soc Journals Ltd, Sheffield",
journal = "European Respiratory Journal",
title = "Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_imagine_675"
}

Milosević, K., Bošković, S., Ljujić, M., Nestorović, B.,& Nikolić, A.. (2013). Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis. in European Respiratory Journal
European Respiratory Soc Journals Ltd, Sheffield., 42.
https://hdl.handle.net/21.15107/rcub_imagine_675

Milosević K, Bošković S, Ljujić M, Nestorović B, Nikolić A. Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis. in European Respiratory Journal. 2013;42.
https://hdl.handle.net/21.15107/rcub_imagine_675 .

Milosević, Katarina, Bošković, Srđan, Ljujić, Mila, Nestorović, Branimir, Nikolić, Aleksandra, "Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis" in European Respiratory Journal, 42 (2013),
https://hdl.handle.net/21.15107/rcub_imagine_675 .

TY  - JOUR
AU  - Milosević, Katarina
AU  - Rsovac, Snežana
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/567
AB  - The aim of this study was to investigate response to salbutamol in children with acute asthma exacerbations in order to estimate the efficiency and safety of its administration in pediatric population. The study included 56 children with asthma (age range 5-12) who were diagnosed and treated over a period of two years. In all children, salbutamol was administered via nebulization, in doses of 1.25 mg in children aged 5-6 and 2.5 mg in children aged 7-12. Respiratory and blood parameters were monitored before and 30 minutes after salbutamol administration. In children with acute asthma exacerbations, respiratory frequency, potassium level, sodium level and PaCO, decreased significantly after salbutamol administration, while PEF, PEF%, glycemia, PaO2 and SatO(2) significantly increased. Hypokalemia due to salbutanzol effect occurred in 15 (26.8%) children. Most significant improvement in oxygenation, with increase of PEF, PEF%, PaO2 and SatO(2), after salbutamol administration was achieved in patients with mild acute exacerbations. Salbulanzol administered via nebulization leads to the occurrence of adverse metabolic elfe cts, hypokalemia and hyperglycemia. Howevez; it can be safely used in the treatment of acute asthma exacerbations and demonstrates high clinical efficacy in the management of acute bronchospasm. The most effective salbutamol treatment is achieved in patients with mild exacerbations.
T2  - Paediatria Croatica
T1  - Response to salbutamol in children with acute asthma exacerbations
EP  - 208
IS  - 3
SP  - 205
VL  - 56
UR  - https://hdl.handle.net/21.15107/rcub_imagine_567
ER  - 

@article{
author = "Milosević, Katarina and Rsovac, Snežana and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2012",
abstract = "The aim of this study was to investigate response to salbutamol in children with acute asthma exacerbations in order to estimate the efficiency and safety of its administration in pediatric population. The study included 56 children with asthma (age range 5-12) who were diagnosed and treated over a period of two years. In all children, salbutamol was administered via nebulization, in doses of 1.25 mg in children aged 5-6 and 2.5 mg in children aged 7-12. Respiratory and blood parameters were monitored before and 30 minutes after salbutamol administration. In children with acute asthma exacerbations, respiratory frequency, potassium level, sodium level and PaCO, decreased significantly after salbutamol administration, while PEF, PEF%, glycemia, PaO2 and SatO(2) significantly increased. Hypokalemia due to salbutanzol effect occurred in 15 (26.8%) children. Most significant improvement in oxygenation, with increase of PEF, PEF%, PaO2 and SatO(2), after salbutamol administration was achieved in patients with mild acute exacerbations. Salbulanzol administered via nebulization leads to the occurrence of adverse metabolic elfe cts, hypokalemia and hyperglycemia. Howevez; it can be safely used in the treatment of acute asthma exacerbations and demonstrates high clinical efficacy in the management of acute bronchospasm. The most effective salbutamol treatment is achieved in patients with mild exacerbations.",
journal = "Paediatria Croatica",
title = "Response to salbutamol in children with acute asthma exacerbations",
pages = "208-205",
number = "3",
volume = "56",
url = "https://hdl.handle.net/21.15107/rcub_imagine_567"
}

Milosević, K., Rsovac, S., Nestorović, B.,& Nikolić, A.. (2012). Response to salbutamol in children with acute asthma exacerbations. in Paediatria Croatica, 56(3), 205-208.
https://hdl.handle.net/21.15107/rcub_imagine_567

Milosević K, Rsovac S, Nestorović B, Nikolić A. Response to salbutamol in children with acute asthma exacerbations. in Paediatria Croatica. 2012;56(3):205-208.
https://hdl.handle.net/21.15107/rcub_imagine_567 .

Milosević, Katarina, Rsovac, Snežana, Nestorović, Branimir, Nikolić, Aleksandra, "Response to salbutamol in children with acute asthma exacerbations" in Paediatria Croatica, 56, no. 3 (2012):205-208,
https://hdl.handle.net/21.15107/rcub_imagine_567 .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Milosević, Katarina
AU  - Divac Rankov, Aleksandra
AU  - Ljujić, Mila
AU  - Grković, Slobodanka
AU  - Nestorović, Branimir
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/466
AB  - This paper reports a novel Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variant, 1811+1G - gt  T, detected in a 5-year-old girl diagnosed with idiopathic disseminated bronchiectasis and negative sweat chloride test (17 mmol/L). The performed CFTR gene mutation analysis included detection of the F508del mutation, analysis of Tn polymorphism and screening of CFTR exons 3, 10 and 11. The CFTR gene screening has shown the altered band pattern in exon 11. The DNA sequencing of CFTR exon 11 revealed the presence of the novel sequence variation 1811+1G - gt  T in heterozygous state. This sequence variation was not found in any of 100 control alleles, analyzed by polymerase chain reaction - restriction fragment length polymorphism method. The novel sequence variation 1811+1G - gt  T is located at the splicing site at the boundary of exon 11 and intron 11 and might be either a sequence variation or a splicing site defect. lt /.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Fetal and Pediatric Pathology
T1  - Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis
EP  - 98
IS  - 2
SP  - 95
VL  - 29
DO  - 10.3109/15513811003620815
ER  - 

@article{
author = "Nikolić, Aleksandra and Milosević, Katarina and Divac Rankov, Aleksandra and Ljujić, Mila and Grković, Slobodanka and Nestorović, Branimir",
year = "2010",
abstract = "This paper reports a novel Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variant, 1811+1G - gt  T, detected in a 5-year-old girl diagnosed with idiopathic disseminated bronchiectasis and negative sweat chloride test (17 mmol/L). The performed CFTR gene mutation analysis included detection of the F508del mutation, analysis of Tn polymorphism and screening of CFTR exons 3, 10 and 11. The CFTR gene screening has shown the altered band pattern in exon 11. The DNA sequencing of CFTR exon 11 revealed the presence of the novel sequence variation 1811+1G - gt  T in heterozygous state. This sequence variation was not found in any of 100 control alleles, analyzed by polymerase chain reaction - restriction fragment length polymorphism method. The novel sequence variation 1811+1G - gt  T is located at the splicing site at the boundary of exon 11 and intron 11 and might be either a sequence variation or a splicing site defect. lt /.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Fetal and Pediatric Pathology",
title = "Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis",
pages = "98-95",
number = "2",
volume = "29",
doi = "10.3109/15513811003620815"
}

Nikolić, A., Milosević, K., Divac Rankov, A., Ljujić, M., Grković, S.,& Nestorović, B.. (2010). Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis. in Fetal and Pediatric Pathology
Taylor & Francis Inc, Philadelphia., 29(2), 95-98.
https://doi.org/10.3109/15513811003620815

Nikolić A, Milosević K, Divac Rankov A, Ljujić M, Grković S, Nestorović B. Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis. in Fetal and Pediatric Pathology. 2010;29(2):95-98.
doi:10.3109/15513811003620815 .

Nikolić, Aleksandra, Milosević, Katarina, Divac Rankov, Aleksandra, Ljujić, Mila, Grković, Slobodanka, Nestorović, Branimir, "Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis" in Fetal and Pediatric Pathology, 29, no. 2 (2010):95-98,
https://doi.org/10.3109/15513811003620815 . .