TY  - JOUR
AU  - Zavisić, Gordana N.
AU  - Petricević, Sasa M.
AU  - Ristić, Slavica M.
AU  - Rikalović, Milena G.
AU  - Jovanović-Ljesković, Nataša M.
AU  - Begović, Jelena
AU  - Strahinić, Ivana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1268
AB  - The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns
EP  - 376
IS  - 4
SP  - 365
VL  - 84
DO  - 10.2298/JSC181105015Z
ER  - 

@article{
author = "Zavisić, Gordana N. and Petricević, Sasa M. and Ristić, Slavica M. and Rikalović, Milena G. and Jovanović-Ljesković, Nataša M. and Begović, Jelena and Strahinić, Ivana",
year = "2019",
abstract = "The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns",
pages = "376-365",
number = "4",
volume = "84",
doi = "10.2298/JSC181105015Z"
}

Zavisić, G. N., Petricević, S. M., Ristić, S. M., Rikalović, M. G., Jovanović-Ljesković, N. M., Begović, J.,& Strahinić, I.. (2019). Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(4), 365-376.
https://doi.org/10.2298/JSC181105015Z

Zavisić GN, Petricević SM, Ristić SM, Rikalović MG, Jovanović-Ljesković NM, Begović J, Strahinić I. Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns. in Journal of the Serbian Chemical Society. 2019;84(4):365-376.
doi:10.2298/JSC181105015Z .

Zavisić, Gordana N., Petricević, Sasa M., Ristić, Slavica M., Rikalović, Milena G., Jovanović-Ljesković, Nataša M., Begović, Jelena, Strahinić, Ivana, "Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns" in Journal of the Serbian Chemical Society, 84, no. 4 (2019):365-376,
https://doi.org/10.2298/JSC181105015Z . .