TY  - CONF
AU  - Nikolić, Ljiljana
AU  - Vidović, Marija
AU  - Todorović, Nataša
AU  - Petković, Branka
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Bogdanović Pristov, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2168
AB  - Investigating membrane properties of plants is a challenging task, considering that success ofexperiments is highly dependent on the possibility to isolate metabolically active protoplasts thatcan withstand membrane current recordings. The aim of the present work is to obtain viableprotoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplastswith preserved membrane integrity suitable for electrophysiological experiments. We applied acustom approach for patch-clamping protoplasts using a microscope with a movable microscopestage. We recorded prominent inward and prominent outward types of membrane current profiles ofprotoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia
T1  - Protoplast patch-clamping using an upright microscope with a movable stage
EP  - 274
SP  - 271
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5508
ER  - 

@conference{
editor = "Čupić, Željko, Anić, Slobodan",
author = "Nikolić, Ljiljana and Vidović, Marija and Todorović, Nataša and Petković, Branka and Stojadinović, Gordana and Martać, Ljiljana and Bogdanović Pristov, Jelena",
year = "2022",
abstract = "Investigating membrane properties of plants is a challenging task, considering that success ofexperiments is highly dependent on the possibility to isolate metabolically active protoplasts thatcan withstand membrane current recordings. The aim of the present work is to obtain viableprotoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplastswith preserved membrane integrity suitable for electrophysiological experiments. We applied acustom approach for patch-clamping protoplasts using a microscope with a movable microscopestage. We recorded prominent inward and prominent outward types of membrane current profiles ofprotoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia",
title = "Protoplast patch-clamping using an upright microscope with a movable stage",
pages = "274-271",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5508"
}

Čupić, Ž., Anić, S., Nikolić, L., Vidović, M., Todorović, N., Petković, B., Stojadinović, G., Martać, L.,& Bogdanović Pristov, J.. (2022). Protoplast patch-clamping using an upright microscope with a movable stage. in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia
Belgrade : Society of Physical Chemists of Serbia., 271-274.
https://hdl.handle.net/21.15107/rcub_ibiss_5508

Čupić Ž, Anić S, Nikolić L, Vidović M, Todorović N, Petković B, Stojadinović G, Martać L, Bogdanović Pristov J. Protoplast patch-clamping using an upright microscope with a movable stage. in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia. 2022;:271-274.
https://hdl.handle.net/21.15107/rcub_ibiss_5508 .

Čupić, Željko, Anić, Slobodan, Nikolić, Ljiljana, Vidović, Marija, Todorović, Nataša, Petković, Branka, Stojadinović, Gordana, Martać, Ljiljana, Bogdanović Pristov, Jelena, "Protoplast patch-clamping using an upright microscope with a movable stage" in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia (2022):271-274,
https://hdl.handle.net/21.15107/rcub_ibiss_5508 .