TY  - JOUR
AU  - Aleksić, Milena
AU  - Stanisavljević Ninković, Danijela
AU  - Smiljković, Marija
AU  - Vasiljević, Perica
AU  - Stevanović, Milena
AU  - Soković, Marina
AU  - Stojković, Dejan
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1283
AB  - Pyrimethanil, a synthetic fungicide widely used for the treatment of pre- and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 mu g mu L-1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng mu L-1, while the expression of Ki67 marker of proliferation was decreased with 150 ng mu L-1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines
EP  - 235
IS  - 2
SP  - 228
VL  - 175
DO  - 10.1111/aab.12532
ER  - 

@article{
author = "Aleksić, Milena and Stanisavljević Ninković, Danijela and Smiljković, Marija and Vasiljević, Perica and Stevanović, Milena and Soković, Marina and Stojković, Dejan",
year = "2019",
abstract = "Pyrimethanil, a synthetic fungicide widely used for the treatment of pre- and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 mu g mu L-1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng mu L-1, while the expression of Ki67 marker of proliferation was decreased with 150 ng mu L-1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines",
pages = "235-228",
number = "2",
volume = "175",
doi = "10.1111/aab.12532"
}

Aleksić, M., Stanisavljević Ninković, D., Smiljković, M., Vasiljević, P., Stevanović, M., Soković, M.,& Stojković, D.. (2019). Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines. in Annals of Applied Biology
Wiley, Hoboken., 175(2), 228-235.
https://doi.org/10.1111/aab.12532

Aleksić M, Stanisavljević Ninković D, Smiljković M, Vasiljević P, Stevanović M, Soković M, Stojković D. Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines. in Annals of Applied Biology. 2019;175(2):228-235.
doi:10.1111/aab.12532 .

Aleksić, Milena, Stanisavljević Ninković, Danijela, Smiljković, Marija, Vasiljević, Perica, Stevanović, Milena, Soković, Marina, Stojković, Dejan, "Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines" in Annals of Applied Biology, 175, no. 2 (2019):228-235,
https://doi.org/10.1111/aab.12532 . .