TY  - JOUR
AU  - Stanisavljević, S.
AU  - Lukić, Jovanka
AU  - Momcilović, M.
AU  - Miljković, M.
AU  - Jevtić, B.
AU  - Kojić, Milan
AU  - Golić, Nataša
AU  - Mostarica Stojković, M.
AU  - Miljković, D.
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/947
AB  - Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund's adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer's patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4(+) T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-gamma and interleukin-17. Finally, microbial analyses showed that uncultivated species of Turicibacter and Atopostipes genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is clear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis.
PB  - Wageningen Academic Publishers, Wageningen
T2  - Beneficial Microbes
T1  - Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis
EP  - 373
IS  - 3
SP  - 363
VL  - 7
DO  - 10.3920/BM2015.0159
ER  - 

@article{
author = "Stanisavljević, S. and Lukić, Jovanka and Momcilović, M. and Miljković, M. and Jevtić, B. and Kojić, Milan and Golić, Nataša and Mostarica Stojković, M. and Miljković, D.",
year = "2016",
abstract = "Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund's adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer's patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4(+) T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-gamma and interleukin-17. Finally, microbial analyses showed that uncultivated species of Turicibacter and Atopostipes genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is clear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "Beneficial Microbes",
title = "Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis",
pages = "373-363",
number = "3",
volume = "7",
doi = "10.3920/BM2015.0159"
}

Stanisavljević, S., Lukić, J., Momcilović, M., Miljković, M., Jevtić, B., Kojić, M., Golić, N., Mostarica Stojković, M.,& Miljković, D.. (2016). Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis. in Beneficial Microbes
Wageningen Academic Publishers, Wageningen., 7(3), 363-373.
https://doi.org/10.3920/BM2015.0159

Stanisavljević S, Lukić J, Momcilović M, Miljković M, Jevtić B, Kojić M, Golić N, Mostarica Stojković M, Miljković D. Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis. in Beneficial Microbes. 2016;7(3):363-373.
doi:10.3920/BM2015.0159 .

Stanisavljević, S., Lukić, Jovanka, Momcilović, M., Miljković, M., Jevtić, B., Kojić, Milan, Golić, Nataša, Mostarica Stojković, M., Miljković, D., "Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis" in Beneficial Microbes, 7, no. 3 (2016):363-373,
https://doi.org/10.3920/BM2015.0159 . .

TY  - JOUR
AU  - Petković, F.
AU  - Zivanović, J.
AU  - Blazevski, J.
AU  - Timotijević, Gordana
AU  - Momcilović, M.
AU  - Stanojević, Z.
AU  - Stamenković, V.
AU  - Milosević, V.
AU  - Mostarica-Stojković, Marija
AU  - Miljković, D.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/844
AB  - Experimental autoimmune encephalomyelitis (EAE) is a model of multiple sclerosis (MS), inflammatory, demyelinating and neurodegenerative disease of the central nervous system (CNS). Clinically manifested EAE can be induced in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats by immunization with spinal cord homogenate (SCH) and complete Freund's adjuvant (CFA). Matrix metalloproteinases (MMP) play important roles in various steps of MS and EAE pathogenesis. Expression of gelatinases MMP2 and MMP9, their activator MMP14 and their inhibitor tissue inhibitor of MMP (TIMP) 1 in the CNS of AO and DA rats immunized with SCH + CFA was determined. Expression of mRNA for MMP2, MMP9 and MMP14 was higher and expression of TIMP1 mRNA was lower in AO rats. However, gelatinase activity in spinal cords was higher in samples obtained from DA rats. Further, while there was no strain difference in MMP2 and MMP9 mRNA expression in lymph nodes of the immunized rats, gelatinase activity was higher in DA rats. This activity was reduced by antiinflammatory cytokines interleukin (IL)-10 and IL-4. Interestingly, gelatinase activity was detected in the nuclei of cells within the CNS, but not of those in lymph nodes. Our results imply that posttranscriptional regulation of MMP2 and MMP9 expression and/or function determines low gelatinase activity within the CNS and in immune cells of EAE-resistant AO rats.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Neuroscience
T1  - Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis
EP  - 12
SP  - 1
VL  - 292
DO  - 10.1016/j.neuroscience.2015.02.015
ER  - 

@article{
author = "Petković, F. and Zivanović, J. and Blazevski, J. and Timotijević, Gordana and Momcilović, M. and Stanojević, Z. and Stamenković, V. and Milosević, V. and Mostarica-Stojković, Marija and Miljković, D.",
year = "2015",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is a model of multiple sclerosis (MS), inflammatory, demyelinating and neurodegenerative disease of the central nervous system (CNS). Clinically manifested EAE can be induced in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats by immunization with spinal cord homogenate (SCH) and complete Freund's adjuvant (CFA). Matrix metalloproteinases (MMP) play important roles in various steps of MS and EAE pathogenesis. Expression of gelatinases MMP2 and MMP9, their activator MMP14 and their inhibitor tissue inhibitor of MMP (TIMP) 1 in the CNS of AO and DA rats immunized with SCH + CFA was determined. Expression of mRNA for MMP2, MMP9 and MMP14 was higher and expression of TIMP1 mRNA was lower in AO rats. However, gelatinase activity in spinal cords was higher in samples obtained from DA rats. Further, while there was no strain difference in MMP2 and MMP9 mRNA expression in lymph nodes of the immunized rats, gelatinase activity was higher in DA rats. This activity was reduced by antiinflammatory cytokines interleukin (IL)-10 and IL-4. Interestingly, gelatinase activity was detected in the nuclei of cells within the CNS, but not of those in lymph nodes. Our results imply that posttranscriptional regulation of MMP2 and MMP9 expression and/or function determines low gelatinase activity within the CNS and in immune cells of EAE-resistant AO rats.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Neuroscience",
title = "Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis",
pages = "12-1",
volume = "292",
doi = "10.1016/j.neuroscience.2015.02.015"
}

Petković, F., Zivanović, J., Blazevski, J., Timotijević, G., Momcilović, M., Stanojević, Z., Stamenković, V., Milosević, V., Mostarica-Stojković, M.,& Miljković, D.. (2015). Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis. in Neuroscience
Pergamon-Elsevier Science Ltd, Oxford., 292, 1-12.
https://doi.org/10.1016/j.neuroscience.2015.02.015

Petković F, Zivanović J, Blazevski J, Timotijević G, Momcilović M, Stanojević Z, Stamenković V, Milosević V, Mostarica-Stojković M, Miljković D. Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis. in Neuroscience. 2015;292:1-12.
doi:10.1016/j.neuroscience.2015.02.015 .

Petković, F., Zivanović, J., Blazevski, J., Timotijević, Gordana, Momcilović, M., Stanojević, Z., Stamenković, V., Milosević, V., Mostarica-Stojković, Marija, Miljković, D., "Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis" in Neuroscience, 292 (2015):1-12,
https://doi.org/10.1016/j.neuroscience.2015.02.015 . .

TY  - JOUR
AU  - Radović, J.
AU  - Maksimović-Ivanić, Danijela
AU  - Timotijević, Gordana
AU  - Popadić, S.
AU  - Ramić, Z.
AU  - Trajković, V.
AU  - Miljković, D.
AU  - Stošić-Grujičić, Stanislava
AU  - Mijatović, S.
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/550
AB  - Intrinsic characteristics of melanoma cells such as expression of inducible nitric oxide synthase (iNOS), redox status, and activity of signaling pathways involved in proliferation, differentiation and cell death define the response of the cells to the diverse treatments. In this context we compared the effectiveness of herbal antaquinone aloe emodin (AE) against mouse B16 melanoma and human A375, different in initial activity of ERK1/2, constitutive iNOS expression and basal level of reactive oxygen species (ROS). Both cell lines are sensitive to AE treatment. However, while the agent induces differentiation of B16 cells toward melanocytes, in A375 cells promoted massive apoptosis. Differentiation of B16 cells, characterized by enhanced melanin production and tyrosinase activity, was mediated by H2O2 production synchronized with rapid p53 accumulation and enhanced expression of cyclins D1 and D3. Caspase mediated apoptosis triggered in A375 cells was accompanied with Bcl-2 but not iNOS down-regulation. In addition, opposite regulation of Akt-ERK1/2 axis in AE treated B16 and A375 cells correlated with different outcome of the treatment. However, AE in a dose-dependent manner rescued both B16 and A375 cells from doxorubicin- or paclitaxel-induced killing. These data indicate that caution is warranted when AE is administrated to the patients with conventional chemotherapy.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Cell-type dependent response of melanoma cells to aloe emodin
EP  - 3189
IS  - 9
SP  - 3181
VL  - 50
DO  - 10.1016/j.fct.2012.05.047
ER  - 

@article{
author = "Radović, J. and Maksimović-Ivanić, Danijela and Timotijević, Gordana and Popadić, S. and Ramić, Z. and Trajković, V. and Miljković, D. and Stošić-Grujičić, Stanislava and Mijatović, S.",
year = "2012",
abstract = "Intrinsic characteristics of melanoma cells such as expression of inducible nitric oxide synthase (iNOS), redox status, and activity of signaling pathways involved in proliferation, differentiation and cell death define the response of the cells to the diverse treatments. In this context we compared the effectiveness of herbal antaquinone aloe emodin (AE) against mouse B16 melanoma and human A375, different in initial activity of ERK1/2, constitutive iNOS expression and basal level of reactive oxygen species (ROS). Both cell lines are sensitive to AE treatment. However, while the agent induces differentiation of B16 cells toward melanocytes, in A375 cells promoted massive apoptosis. Differentiation of B16 cells, characterized by enhanced melanin production and tyrosinase activity, was mediated by H2O2 production synchronized with rapid p53 accumulation and enhanced expression of cyclins D1 and D3. Caspase mediated apoptosis triggered in A375 cells was accompanied with Bcl-2 but not iNOS down-regulation. In addition, opposite regulation of Akt-ERK1/2 axis in AE treated B16 and A375 cells correlated with different outcome of the treatment. However, AE in a dose-dependent manner rescued both B16 and A375 cells from doxorubicin- or paclitaxel-induced killing. These data indicate that caution is warranted when AE is administrated to the patients with conventional chemotherapy.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Cell-type dependent response of melanoma cells to aloe emodin",
pages = "3189-3181",
number = "9",
volume = "50",
doi = "10.1016/j.fct.2012.05.047"
}

Radović, J., Maksimović-Ivanić, D., Timotijević, G., Popadić, S., Ramić, Z., Trajković, V., Miljković, D., Stošić-Grujičić, S.,& Mijatović, S.. (2012). Cell-type dependent response of melanoma cells to aloe emodin. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 50(9), 3181-3189.
https://doi.org/10.1016/j.fct.2012.05.047

Radović J, Maksimović-Ivanić D, Timotijević G, Popadić S, Ramić Z, Trajković V, Miljković D, Stošić-Grujičić S, Mijatović S. Cell-type dependent response of melanoma cells to aloe emodin. in Food and Chemical Toxicology. 2012;50(9):3181-3189.
doi:10.1016/j.fct.2012.05.047 .

Radović, J., Maksimović-Ivanić, Danijela, Timotijević, Gordana, Popadić, S., Ramić, Z., Trajković, V., Miljković, D., Stošić-Grujičić, Stanislava, Mijatović, S., "Cell-type dependent response of melanoma cells to aloe emodin" in Food and Chemical Toxicology, 50, no. 9 (2012):3181-3189,
https://doi.org/10.1016/j.fct.2012.05.047 . .

TY  - CONF
AU  - Mijatović, S.
AU  - Radović, J.
AU  - Timotijević, Gordana
AU  - Mojić, M.
AU  - Miljković, D.
AU  - Dekanski, D.
AU  - Stošić-Grujičić, Stanislava
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/349
PB  - Georg Thieme Verlag Kg, Stuttgart
C3  - Planta Medica
T1  - Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway
EP  - 903
IS  - 9
SP  - 903
VL  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1443
ER  - 

@conference{
author = "Mijatović, S. and Radović, J. and Timotijević, Gordana and Mojić, M. and Miljković, D. and Dekanski, D. and Stošić-Grujičić, Stanislava",
year = "2009",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway",
pages = "903-903",
number = "9",
volume = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1443"
}

Mijatović, S., Radović, J., Timotijević, G., Mojić, M., Miljković, D., Dekanski, D.,& Stošić-Grujičić, S.. (2009). Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway. in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 75(9), 903-903.
https://hdl.handle.net/21.15107/rcub_ibiss_1443

Mijatović S, Radović J, Timotijević G, Mojić M, Miljković D, Dekanski D, Stošić-Grujičić S. Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway. in Planta Medica. 2009;75(9):903-903.
https://hdl.handle.net/21.15107/rcub_ibiss_1443 .

Mijatović, S., Radović, J., Timotijević, Gordana, Mojić, M., Miljković, D., Dekanski, D., Stošić-Grujičić, Stanislava, "Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway" in Planta Medica, 75, no. 9 (2009):903-903,
https://hdl.handle.net/21.15107/rcub_ibiss_1443 .

TY  - JOUR
AU  - Miljković, D
AU  - Samardzić, T
AU  - Drakulić, Danijela
AU  - Stošić-Grujičić, Stanislava
AU  - Trajković, V
PY  - 2002
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/162
AB  - Mycophenolic acid (MPA) and A77 1726, the active components of the immunosuppressants mycophenolate mophetil and leflunomide, respectively, in a dose-dependent manner inhibited interferon (IFN)-gamma/LPS-induced interleukin (IL)-6 release in confluent cultures of mouse L929 fibrosarcoma cells. In addition, both drugs markedly reduced the production of the free radical gas nitric oxide (NO), without affecting the viability of L929 cells. The inhibitors of NO synthase, aminoguanidine and L-NMMA, but not L-NMMA inactive counterpart D-NMMA, mimicked the effects of A77 1726 and MPA on IL-6 generation in L929 fibroblasts. Furthermore, NO-releasing substance SNP completely reverted IL-6 accumulation in L929 cultures treated with A77 1726, while only partial recovery of IL-6 production was observed in the presence of MPA. MPA, but not A77 1726, significantly suppressed NO-independent IL-6 release triggered by cAMP-elevating agent rolipram. Thus, while A77 1726 effect on IL-6 production was mediated through concomitant reduction of NO synthesis, MPA action was mainly independent of the interference with NO generation. Finally, both agents inhibited IFN-gamma/LPS-triggered IL-6 production in mouse primary fibroblasts, but not in mouse peritoneal macrophages, indicating cell-specificity of this novel anti-inflammatory action of A77 1726 and MPA.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Cytokine
T1  - Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms
EP  - 186
IS  - 4
SP  - 181
VL  - 19
DO  - 10.1006/cyto.2002.0885
ER  - 

@article{
author = "Miljković, D and Samardzić, T and Drakulić, Danijela and Stošić-Grujičić, Stanislava and Trajković, V",
year = "2002",
abstract = "Mycophenolic acid (MPA) and A77 1726, the active components of the immunosuppressants mycophenolate mophetil and leflunomide, respectively, in a dose-dependent manner inhibited interferon (IFN)-gamma/LPS-induced interleukin (IL)-6 release in confluent cultures of mouse L929 fibrosarcoma cells. In addition, both drugs markedly reduced the production of the free radical gas nitric oxide (NO), without affecting the viability of L929 cells. The inhibitors of NO synthase, aminoguanidine and L-NMMA, but not L-NMMA inactive counterpart D-NMMA, mimicked the effects of A77 1726 and MPA on IL-6 generation in L929 fibroblasts. Furthermore, NO-releasing substance SNP completely reverted IL-6 accumulation in L929 cultures treated with A77 1726, while only partial recovery of IL-6 production was observed in the presence of MPA. MPA, but not A77 1726, significantly suppressed NO-independent IL-6 release triggered by cAMP-elevating agent rolipram. Thus, while A77 1726 effect on IL-6 production was mediated through concomitant reduction of NO synthesis, MPA action was mainly independent of the interference with NO generation. Finally, both agents inhibited IFN-gamma/LPS-triggered IL-6 production in mouse primary fibroblasts, but not in mouse peritoneal macrophages, indicating cell-specificity of this novel anti-inflammatory action of A77 1726 and MPA.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Cytokine",
title = "Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms",
pages = "186-181",
number = "4",
volume = "19",
doi = "10.1006/cyto.2002.0885"
}

Miljković, D., Samardzić, T., Drakulić, D., Stošić-Grujičić, S.,& Trajković, V.. (2002). Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms. in Cytokine
Academic Press Ltd- Elsevier Science Ltd, London., 19(4), 181-186.
https://doi.org/10.1006/cyto.2002.0885

Miljković D, Samardzić T, Drakulić D, Stošić-Grujičić S, Trajković V. Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms. in Cytokine. 2002;19(4):181-186.
doi:10.1006/cyto.2002.0885 .

Miljković, D, Samardzić, T, Drakulić, Danijela, Stošić-Grujičić, Stanislava, Trajković, V, "Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms" in Cytokine, 19, no. 4 (2002):181-186,
https://doi.org/10.1006/cyto.2002.0885 . .