TY  - JOUR
AU  - Elez-Burnjaković, Nikolina
AU  - Ugrin, Milena
AU  - Obradović, Jasmina
AU  - Miletić, Nataša
AU  - Racić, Maja
AU  - Kulić, Milan
AU  - Pavlović, Sonja
AU  - Jurisić, Vladimir
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1164
AB  - Purpose: To analyze the frequencies of two single nucleotide polymorphisms (SNPs) of EGFR gene, -191C/A and 181946G/A, among lung cancer patients from the Republic of Srpska, Bosnia and Hercegovina, as well as to assess the association of SNP genotypes with the cancer type and other demographic characteristics of patients, particularly with the smoking status. Methods: This study enrolled 41 lung cancer patients from the territory of Republic Srpska, Bosnia and Herzegovina. Detection EGFR SNPs was performed using PCR-RFLP methodology. PCR was performed on 2720 Thermal Cycler (Applied Biosystems, United States). PCR, as well as RFLP products, were detected by gel electrophoresis. SPSS-17 soft-ware (SPSS, Inc.) was used for statistical analyses. Results: There was significantly more male than female smokers in our cohort (p=0.006). In addition, the proportion of smokers was higher among patients with adenocarcinoma in comparison to patients with other lung cancer types p=0.044). Adenocarcinoma was less common in patients older than 64 years (p=0.035). The wild type homozygous genotype of both SNPs was the most frequent genotype in all the tested demographic groups. Using dominant genetic model for -191C/A SNP, we observed statistically significant association of -191CC genotype and adenocarcinoma (p=0.043) in the subgroup of patients younger than 64 years. Namely, patients younger than 64 years and carriers of -191CC genotype had higher risk (odds ratio/OR=9.6; 95% confidence interval/CI=0.8477 to 108.7214) for adenocarcinoma than the ones carrying -191CA or -191AA genotype. Conclusions: Patients younger than 64 years and carriers of -191CC genotype have significantly higher risk for adenocarcinoma than carriers of -191CA or -191AA genotype. Further studies on larger cohorts are necessary to evaluate -191C/A SNP as a potential biomarker.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of Buon
T1  - Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina
EP  - 390
IS  - 2
SP  - 384
VL  - 23
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1164
ER  - 

@article{
author = "Elez-Burnjaković, Nikolina and Ugrin, Milena and Obradović, Jasmina and Miletić, Nataša and Racić, Maja and Kulić, Milan and Pavlović, Sonja and Jurisić, Vladimir",
year = "2018",
abstract = "Purpose: To analyze the frequencies of two single nucleotide polymorphisms (SNPs) of EGFR gene, -191C/A and 181946G/A, among lung cancer patients from the Republic of Srpska, Bosnia and Hercegovina, as well as to assess the association of SNP genotypes with the cancer type and other demographic characteristics of patients, particularly with the smoking status. Methods: This study enrolled 41 lung cancer patients from the territory of Republic Srpska, Bosnia and Herzegovina. Detection EGFR SNPs was performed using PCR-RFLP methodology. PCR was performed on 2720 Thermal Cycler (Applied Biosystems, United States). PCR, as well as RFLP products, were detected by gel electrophoresis. SPSS-17 soft-ware (SPSS, Inc.) was used for statistical analyses. Results: There was significantly more male than female smokers in our cohort (p=0.006). In addition, the proportion of smokers was higher among patients with adenocarcinoma in comparison to patients with other lung cancer types p=0.044). Adenocarcinoma was less common in patients older than 64 years (p=0.035). The wild type homozygous genotype of both SNPs was the most frequent genotype in all the tested demographic groups. Using dominant genetic model for -191C/A SNP, we observed statistically significant association of -191CC genotype and adenocarcinoma (p=0.043) in the subgroup of patients younger than 64 years. Namely, patients younger than 64 years and carriers of -191CC genotype had higher risk (odds ratio/OR=9.6; 95% confidence interval/CI=0.8477 to 108.7214) for adenocarcinoma than the ones carrying -191CA or -191AA genotype. Conclusions: Patients younger than 64 years and carriers of -191CC genotype have significantly higher risk for adenocarcinoma than carriers of -191CA or -191AA genotype. Further studies on larger cohorts are necessary to evaluate -191C/A SNP as a potential biomarker.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of Buon",
title = "Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina",
pages = "390-384",
number = "2",
volume = "23",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1164"
}

Elez-Burnjaković, N., Ugrin, M., Obradović, J., Miletić, N., Racić, M., Kulić, M., Pavlović, S.,& Jurisić, V.. (2018). Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina. in Journal of Buon
Balkan Union of Oncology (B.U.ON.)., 23(2), 384-390.
https://hdl.handle.net/21.15107/rcub_imagine_1164

Elez-Burnjaković N, Ugrin M, Obradović J, Miletić N, Racić M, Kulić M, Pavlović S, Jurisić V. Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina. in Journal of Buon. 2018;23(2):384-390.
https://hdl.handle.net/21.15107/rcub_imagine_1164 .

Elez-Burnjaković, Nikolina, Ugrin, Milena, Obradović, Jasmina, Miletić, Nataša, Racić, Maja, Kulić, Milan, Pavlović, Sonja, Jurisić, Vladimir, "Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina" in Journal of Buon, 23, no. 2 (2018):384-390,
https://hdl.handle.net/21.15107/rcub_imagine_1164 .

TY  - JOUR
AU  - Jurisić, Vladimir
AU  - Obradović, Jasmina
AU  - Tošić, Nataša
AU  - Pavlović, Sonja
AU  - Kulić, Milan
AU  - Đorđević, Nataša
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/903
AB  - The aim of the study was to examine the effects of frequently used polymerase chain reaction (PCR) additives DMSO, glycerol and betaine on amplification of GC-rich epidermal growth factor receptor (EGFR) gene promoter region, in order to detect the presence of -216G  gt  T and -191C  gt  A gene variations in non-small-cell lung cancer (NSCLC) patients. PCR products and restriction fragments were detected by electrophoresis on 8% polyacrylamide gel and 3% agarose gel. Our analysis shows that single used additives including DMSO in concentration of 7% and 10%, glycerol in concentration of 10%, 15% and 20%, as well as betaine in concentration of 1 M, 1.5 M and 2 M significantly enhanced the yield and specificity of PCR reaction. In addition, the combination of 10% DMSO with 15% glycerol has shown positive effects, whereas other analyzed combinations of additives failed to amplify the EGFR promoter region.
PB  - Elsevier, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients
EP  - 279
SP  - 275
VL  - 128
DO  - 10.1016/j.jpba.2016.05.010
ER  - 

@article{
author = "Jurisić, Vladimir and Obradović, Jasmina and Tošić, Nataša and Pavlović, Sonja and Kulić, Milan and Đorđević, Nataša",
year = "2016",
abstract = "The aim of the study was to examine the effects of frequently used polymerase chain reaction (PCR) additives DMSO, glycerol and betaine on amplification of GC-rich epidermal growth factor receptor (EGFR) gene promoter region, in order to detect the presence of -216G  gt  T and -191C  gt  A gene variations in non-small-cell lung cancer (NSCLC) patients. PCR products and restriction fragments were detected by electrophoresis on 8% polyacrylamide gel and 3% agarose gel. Our analysis shows that single used additives including DMSO in concentration of 7% and 10%, glycerol in concentration of 10%, 15% and 20%, as well as betaine in concentration of 1 M, 1.5 M and 2 M significantly enhanced the yield and specificity of PCR reaction. In addition, the combination of 10% DMSO with 15% glycerol has shown positive effects, whereas other analyzed combinations of additives failed to amplify the EGFR promoter region.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients",
pages = "279-275",
volume = "128",
doi = "10.1016/j.jpba.2016.05.010"
}

Jurisić, V., Obradović, J., Tošić, N., Pavlović, S., Kulić, M.,& Đorđević, N.. (2016). Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier, Amsterdam., 128, 275-279.
https://doi.org/10.1016/j.jpba.2016.05.010

Jurisić V, Obradović J, Tošić N, Pavlović S, Kulić M, Đorđević N. Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients. in Journal of Pharmaceutical and Biomedical Analysis. 2016;128:275-279.
doi:10.1016/j.jpba.2016.05.010 .

Jurisić, Vladimir, Obradović, Jasmina, Tošić, Nataša, Pavlović, Sonja, Kulić, Milan, Đorđević, Nataša, "Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients" in Journal of Pharmaceutical and Biomedical Analysis, 128 (2016):275-279,
https://doi.org/10.1016/j.jpba.2016.05.010 . .