@article{
author = "Košarčić, Slavica and Stevanović, Milena and Došen, Radoslav and Kovačević, Mira and Gagrčin, Mladen and Košarčić, Dušan and Stajić, Ljiljana and Đisalov, Dane",
year = "2005",
abstract = "Iznenadna uginuća svinja izazvana stresom, na farmama pričinjavaju velike gubitke i utiču na rentabilnost proizvodnje. Stoga smo sproveli istraživanje ove pojave na tri farme. Prvi rezultati datiraju od uvođenja halotan-testa 1974. godine, do DNK- testa i sigurne potvrde daje stres-sindrom svinja nasledno oboljenje životinja koje na šestom hromozomu nose tačkastu mutaciju. Navedene činjenice opredelile su cilj naših istraživanja da utvrdimo trenutno stanje tačkaste mutacije na našim farmama, kakav je stepen učestalosti pojavljivanja homozigota i heterozigota, provera halotan testa na molekularnom nivou i predlog programa selekcije za kontrolu osetljivosti na stres. Testirani su hibridi iz uobičajenog programa ukrštanja na našim farmama i obuhvaćeno je 500 prasadi. Utvrdili smo da je učestalost homozigota oko 23 posto i da je zastupljenost različita od, 2 posto, 9 posto do 12 posto. Halotan pozitivne jedinke testirali smo PCR-RFLP metodom molekularne genetike i potvrdili bolesne genotipove T/T. Odabranim životinjama je urađen DNK-test i utvrdili smo 31 posto heterozigota. Učestalost pojavljivanja po farmama je 6 posto, 11 posto i 14 posto. Prema dobijenim rezultatima neophodna je kontrola svih životinja koje se uvode u reproduktivnu eksploataciju, eradikacija homozigota u testu i dozirana pravilna hibridizacija heterozigota, radi kontrolisanja i upravljanja poznatim genima., Sudden stress-induced deaths of swine on farms cause severe losses and affect feasibility of production. That is why we conducted investigations of this occurrence on three farms. The first results were from the time of the introduction of the halothane test in 1974, to the DNA test, and the definite confirmation that the stress syndrome of pigs is an inherited disease of animals that have a point mutation on the 6th chromosome. The listed facts set the objective of our investigations - to determine the current state of the point mutation in our farms, to determine the rate of incidence of homozygotes and heterozygotes, to verify the halothane test at molecular level, and to make a draft program for selection in controlling stress sensitivity. The testing covered hybrids from the usual cross-breeding program in our farms and it was performed on 500 piglets. We established that the incidence of homozygotes was about 23%, and that their representation differed, being 2%, 9%, up to 12%. We tested halothane-positive animals using the PCR-RFLP molecular genetics method and confirmed altered genotypes T/T. The DNA test was performed on selected animals and we established 31% heterozygotes. The incidence in farms was 6% 11% and 14%. The obtained results indicate that it is necessary to control all animals that are being introduced into the reproduction process, to eradicate the homozygote in the test and to secure dose-regulated hybridization of heterozygotes, with the objective to secure control and management of known genes.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Klasične i savremene metode u otkrivanju svinja osetljivih na stres, Conventional and modern methods for detecting stress sensitive swine",
pages = "242-237",
number = "supl. 1-2",
volume = "59",
url = "https://hdl.handle.net/21.15107/rcub_imagine_227"
}
