TY  - CONF
AU  - Plačkić, Nikola
AU  - Kljajević, Nemanja
AU  - Obradović, Mina
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2383
AB  - Anti-microbial drug resistance (AMR) is one of
the global health threats caused by the misuse
of drugs typically used to treat microbial
infections in humans, animals and plants. AMR
in nosocomial infections not only significantly
hinders treatment and endangers the patients’
lives, but also elevates the costs of healthcare.
Multiple research approaches have been initiated
to combat AMR, and one promising method
is bacteriophage therapy. Bacteriophages (phages)
are viruses that naturally exploit bacteria as
their hosts for replication and can cause cell lysis,
which makes them promising candidates for
treating the infections that do not respond to
conventional antibiotic therapies. In this study,
we screened wastewater samples from four
different collectors in Belgrade urban area for
bacteriophages active against clinically isolated
strains of two biofilm-producing bacteria that
readily persist in hospital environment - Klebsiella
pneumoniae (6 strains) and Pseudomonas aeruginosa
(2 strains). Wastewaters were screened
for phage presence through phage enrichment
process, in which bacteria were grown in a mixture
of water samples and nutrient-rich broth.
Obtained cultures were screened for antimicrobial
activity against the respective host strains,
and candidates were subjected to a first-round
plaque assay to detect the phages. Finally, the
activity of all the candidates was tested against
all strains of the same species to gain the first insight
into their host range. We discovered 20 potentially
distinct bacteriophages active against
K. pneumoniae strains and two potentially different
candidates targeting P. aeruginosa. Notably,
one phage exhibited activity against all tested K.
pneumoniae strains, and four were active against
5 out of 6 tested strains. Among 22 candidates in
total, five showed depolymerizing activity, indicating
promise in combating biofilm formation.
Currently, isolation of new phages, as well as purification
and host range analysis is underway for
several candidates targeting K. pneumoniae and
two targeting P. aeruginosa strains.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS
EP  - 148
SP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2383
ER  - 

@conference{
author = "Plačkić, Nikola and Kljajević, Nemanja and Obradović, Mina and Kekić, Dušan and Gajić, Ina and Stanisavljević, Nemanja and Vukotić, Goran",
year = "2024",
abstract = "Anti-microbial drug resistance (AMR) is one of
the global health threats caused by the misuse
of drugs typically used to treat microbial
infections in humans, animals and plants. AMR
in nosocomial infections not only significantly
hinders treatment and endangers the patients’
lives, but also elevates the costs of healthcare.
Multiple research approaches have been initiated
to combat AMR, and one promising method
is bacteriophage therapy. Bacteriophages (phages)
are viruses that naturally exploit bacteria as
their hosts for replication and can cause cell lysis,
which makes them promising candidates for
treating the infections that do not respond to
conventional antibiotic therapies. In this study,
we screened wastewater samples from four
different collectors in Belgrade urban area for
bacteriophages active against clinically isolated
strains of two biofilm-producing bacteria that
readily persist in hospital environment - Klebsiella
pneumoniae (6 strains) and Pseudomonas aeruginosa
(2 strains). Wastewaters were screened
for phage presence through phage enrichment
process, in which bacteria were grown in a mixture
of water samples and nutrient-rich broth.
Obtained cultures were screened for antimicrobial
activity against the respective host strains,
and candidates were subjected to a first-round
plaque assay to detect the phages. Finally, the
activity of all the candidates was tested against
all strains of the same species to gain the first insight
into their host range. We discovered 20 potentially
distinct bacteriophages active against
K. pneumoniae strains and two potentially different
candidates targeting P. aeruginosa. Notably,
one phage exhibited activity against all tested K.
pneumoniae strains, and four were active against
5 out of 6 tested strains. Among 22 candidates in
total, five showed depolymerizing activity, indicating
promise in combating biofilm formation.
Currently, isolation of new phages, as well as purification
and host range analysis is underway for
several candidates targeting K. pneumoniae and
two targeting P. aeruginosa strains.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS",
pages = "148-148",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2383"
}

Plačkić, N., Kljajević, N., Obradović, M., Kekić, D., Gajić, I., Stanisavljević, N.,& Vukotić, G.. (2024). NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 148-148.
https://hdl.handle.net/21.15107/rcub_imagine_2383

Plačkić N, Kljajević N, Obradović M, Kekić D, Gajić I, Stanisavljević N, Vukotić G. NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:148-148.
https://hdl.handle.net/21.15107/rcub_imagine_2383 .

Plačkić, Nikola, Kljajević, Nemanja, Obradović, Mina, Kekić, Dušan, Gajić, Ina, Stanisavljević, Nemanja, Vukotić, Goran, "NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):148-148,
https://hdl.handle.net/21.15107/rcub_imagine_2383 .