TY  - JOUR
AU  - Dragasević, Sanja
AU  - Stanković, Biljana
AU  - Kotur, Nikola
AU  - Sokić-Milutinović, Aleksandra
AU  - Milovanović, Tamara
AU  - Lukić, Snežana
AU  - Milosavljević, Tomica
AU  - Srzentić Dražilov, Sanja
AU  - Klaassen, Kristel
AU  - Pavlović, Sonja
AU  - Popović, Dragan
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1398
AB  - Background: This study analyzed poorly understood relationship of two overlapping conditions: metabolic syndrome (MeS) and inflammatory bowel disease (IBD), both associated with inflammation in the visceral adipose tissue. Methods: Newly diagnosed 104 IBD patients, of which 50 Crohn's disease (CD) and 54 ulcerative colitis (UC), and 45 non-IBD controls were examined for MeS-related obesity and lipid markers. Th-17 immune genes IL17A, IL17F, IL23A, and TLR9 mRNAs were measured in intestinal mucosa by qRT-PCR. Subjects were genotyped for obesity-associated FTO variant rs9939609 by polymerase chain reaction-amplification refractory mutation system. Results: CD was associated with MeS (P = 0.01), while both CD and UC were associated with central obesity (P = 10(-5), P = 0.002, respectively) and low levels of high-density lipoprotein (HDL) cholesterol (P = 5 x 10(-6), P = 6 x 10(-6), respectively). IBD lipid profile was characterized by decreased total and HDL cholesterol, while low-density lipoprotein cholesterol was reduced only in CD. Negative correlations were found between total cholesterol and CD activity index (P = 0.005), waist circumference and IL17A as well as IL17F mRNA levels in inflamed CD colon (P = 0.003, P = 0.001, respectively). Carriers of FTO rs9939609 AA genotype showed increased risk of CD (OR 2.6, P = 0.01). Conclusions: MeS, central obesity, and dyslipidemia could be important for IBD pathogenesis. This could influence therapeutic approaches and prevention strategies in high-risk groups.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Metabolic Syndrome and Related Disorders
T1  - Metabolic Syndrome in Inflammatory Bowel Disease: Association with Genetic Markers of Obesity and Inflammation
EP  - 38
IS  - 1
SP  - 31
VL  - 18
DO  - 10.1089/met.2019.0090
ER  - 

@article{
author = "Dragasević, Sanja and Stanković, Biljana and Kotur, Nikola and Sokić-Milutinović, Aleksandra and Milovanović, Tamara and Lukić, Snežana and Milosavljević, Tomica and Srzentić Dražilov, Sanja and Klaassen, Kristel and Pavlović, Sonja and Popović, Dragan",
year = "2020",
abstract = "Background: This study analyzed poorly understood relationship of two overlapping conditions: metabolic syndrome (MeS) and inflammatory bowel disease (IBD), both associated with inflammation in the visceral adipose tissue. Methods: Newly diagnosed 104 IBD patients, of which 50 Crohn's disease (CD) and 54 ulcerative colitis (UC), and 45 non-IBD controls were examined for MeS-related obesity and lipid markers. Th-17 immune genes IL17A, IL17F, IL23A, and TLR9 mRNAs were measured in intestinal mucosa by qRT-PCR. Subjects were genotyped for obesity-associated FTO variant rs9939609 by polymerase chain reaction-amplification refractory mutation system. Results: CD was associated with MeS (P = 0.01), while both CD and UC were associated with central obesity (P = 10(-5), P = 0.002, respectively) and low levels of high-density lipoprotein (HDL) cholesterol (P = 5 x 10(-6), P = 6 x 10(-6), respectively). IBD lipid profile was characterized by decreased total and HDL cholesterol, while low-density lipoprotein cholesterol was reduced only in CD. Negative correlations were found between total cholesterol and CD activity index (P = 0.005), waist circumference and IL17A as well as IL17F mRNA levels in inflamed CD colon (P = 0.003, P = 0.001, respectively). Carriers of FTO rs9939609 AA genotype showed increased risk of CD (OR 2.6, P = 0.01). Conclusions: MeS, central obesity, and dyslipidemia could be important for IBD pathogenesis. This could influence therapeutic approaches and prevention strategies in high-risk groups.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Metabolic Syndrome and Related Disorders",
title = "Metabolic Syndrome in Inflammatory Bowel Disease: Association with Genetic Markers of Obesity and Inflammation",
pages = "38-31",
number = "1",
volume = "18",
doi = "10.1089/met.2019.0090"
}

Dragasević, S., Stanković, B., Kotur, N., Sokić-Milutinović, A., Milovanović, T., Lukić, S., Milosavljević, T., Srzentić Dražilov, S., Klaassen, K., Pavlović, S.,& Popović, D.. (2020). Metabolic Syndrome in Inflammatory Bowel Disease: Association with Genetic Markers of Obesity and Inflammation. in Metabolic Syndrome and Related Disorders
Mary Ann Liebert, Inc, New Rochelle., 18(1), 31-38.
https://doi.org/10.1089/met.2019.0090

Dragasević S, Stanković B, Kotur N, Sokić-Milutinović A, Milovanović T, Lukić S, Milosavljević T, Srzentić Dražilov S, Klaassen K, Pavlović S, Popović D. Metabolic Syndrome in Inflammatory Bowel Disease: Association with Genetic Markers of Obesity and Inflammation. in Metabolic Syndrome and Related Disorders. 2020;18(1):31-38.
doi:10.1089/met.2019.0090 .

Dragasević, Sanja, Stanković, Biljana, Kotur, Nikola, Sokić-Milutinović, Aleksandra, Milovanović, Tamara, Lukić, Snežana, Milosavljević, Tomica, Srzentić Dražilov, Sanja, Klaassen, Kristel, Pavlović, Sonja, Popović, Dragan, "Metabolic Syndrome in Inflammatory Bowel Disease: Association with Genetic Markers of Obesity and Inflammation" in Metabolic Syndrome and Related Disorders, 18, no. 1 (2020):31-38,
https://doi.org/10.1089/met.2019.0090 . .

TY  - JOUR
AU  - Stanković, Biljana
AU  - Dragasević, Sanja
AU  - Klaassen, Kristel
AU  - Kotur, Nikola
AU  - Srzentić Dražilov, Sanja
AU  - Zukić, Branka
AU  - Sokic Milutinović, Aleksandra
AU  - Milovanović, Tamara
AU  - Lukić, Snežana
AU  - Popović, Dragan
AU  - Pavlović, Sonja
AU  - Nikčević, Gordana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1329
AB  - Background: Crohn's disease (CD) is chronic inflammatory bowel disease with different phenotypic characteristics influencing disease prognosis and therapeutic strategies. The aim of this pilot study was to analyze selected inflammatory and apoptotic markers in non-inflamed and inflamed samples of ileal mucosa of non-stricturing/non-penetrating (NS/NP) and stricturing (S) CD mucosal phenotypes in order to characterize their distinct profiles. Methods: From twenty CD patients (9 NS/NP, 11 S) paired non-inflamed and inflamed ileal biopsies were collected and used for analysis of cytokine (TNF and IL6) and apoptotic (Bcl2, Box, Fas and FasL) genes' expression levels by real-time PCR, while NF kappa B transcriptional potency was assessed by electromobility gel shift assay. Results: Our results demonstrated significant upregulation of TNF and IL6 in inflamed area of both NS/NP (p = 0.03, p = 0.01) and S phenotypes (p = 0.04, p = 0.04), respectively. However, TNF increase was more prominent in NS/NP compared to S inflamed mucosa (p = 0.02). Also, level of proapoptotic Box was significantly higher in NS/NP compared to S inflamed mucosa (p = 0.01). Opposing transcription potency of NF kappa B has been detected between two phenotypes: being decreased in NS/NP (p = 0.07) and increased in S (p = 0.1) inflamed compared to non-inflamed mucosa, demonstrating trend towards statistical significance. Conclusions: We found that two distinct CD phenotypes have specific molecular signatures. Obtained results could direct improvement of current and development of new therapeutic strategies based on more specific molecular stratification of CD patients.
PB  - Elsevier Gmbh, Munich
T2  - Pathology Research and Practice
T1  - Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy
IS  - 6
VL  - 216
DO  - 10.1016/j.prp.2020.152945
ER  - 

@article{
author = "Stanković, Biljana and Dragasević, Sanja and Klaassen, Kristel and Kotur, Nikola and Srzentić Dražilov, Sanja and Zukić, Branka and Sokic Milutinović, Aleksandra and Milovanović, Tamara and Lukić, Snežana and Popović, Dragan and Pavlović, Sonja and Nikčević, Gordana",
year = "2020",
abstract = "Background: Crohn's disease (CD) is chronic inflammatory bowel disease with different phenotypic characteristics influencing disease prognosis and therapeutic strategies. The aim of this pilot study was to analyze selected inflammatory and apoptotic markers in non-inflamed and inflamed samples of ileal mucosa of non-stricturing/non-penetrating (NS/NP) and stricturing (S) CD mucosal phenotypes in order to characterize their distinct profiles. Methods: From twenty CD patients (9 NS/NP, 11 S) paired non-inflamed and inflamed ileal biopsies were collected and used for analysis of cytokine (TNF and IL6) and apoptotic (Bcl2, Box, Fas and FasL) genes' expression levels by real-time PCR, while NF kappa B transcriptional potency was assessed by electromobility gel shift assay. Results: Our results demonstrated significant upregulation of TNF and IL6 in inflamed area of both NS/NP (p = 0.03, p = 0.01) and S phenotypes (p = 0.04, p = 0.04), respectively. However, TNF increase was more prominent in NS/NP compared to S inflamed mucosa (p = 0.02). Also, level of proapoptotic Box was significantly higher in NS/NP compared to S inflamed mucosa (p = 0.01). Opposing transcription potency of NF kappa B has been detected between two phenotypes: being decreased in NS/NP (p = 0.07) and increased in S (p = 0.1) inflamed compared to non-inflamed mucosa, demonstrating trend towards statistical significance. Conclusions: We found that two distinct CD phenotypes have specific molecular signatures. Obtained results could direct improvement of current and development of new therapeutic strategies based on more specific molecular stratification of CD patients.",
publisher = "Elsevier Gmbh, Munich",
journal = "Pathology Research and Practice",
title = "Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy",
number = "6",
volume = "216",
doi = "10.1016/j.prp.2020.152945"
}

Stanković, B., Dragasević, S., Klaassen, K., Kotur, N., Srzentić Dražilov, S., Zukić, B., Sokic Milutinović, A., Milovanović, T., Lukić, S., Popović, D., Pavlović, S.,& Nikčević, G.. (2020). Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy. in Pathology Research and Practice
Elsevier Gmbh, Munich., 216(6).
https://doi.org/10.1016/j.prp.2020.152945

Stanković B, Dragasević S, Klaassen K, Kotur N, Srzentić Dražilov S, Zukić B, Sokic Milutinović A, Milovanović T, Lukić S, Popović D, Pavlović S, Nikčević G. Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy. in Pathology Research and Practice. 2020;216(6).
doi:10.1016/j.prp.2020.152945 .

Stanković, Biljana, Dragasević, Sanja, Klaassen, Kristel, Kotur, Nikola, Srzentić Dražilov, Sanja, Zukić, Branka, Sokic Milutinović, Aleksandra, Milovanović, Tamara, Lukić, Snežana, Popović, Dragan, Pavlović, Sonja, Nikčević, Gordana, "Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy" in Pathology Research and Practice, 216, no. 6 (2020),
https://doi.org/10.1016/j.prp.2020.152945 . .

TY  - JOUR
AU  - Dragasević, Sanja
AU  - Stanković, Biljana
AU  - Sokić-Milutinović, Aleksandra
AU  - Milosavljević, Tomica
AU  - Milovanović, Tamara
AU  - Lukić, Snežana
AU  - Srzentić Dražilov, Sanja
AU  - Klaassen, Kristel
AU  - Kotur, Nikola
AU  - Pavlović, Sonja
AU  - Popović, Dragan
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1123
AB  - Background and aims: Mucosal gene expression have not been fully enlightened in inflammatory bowel disease (IBD). Aim of this study was to define IL23A, IL17A, IL17F and TLR9 expression in different IBD phenotypes. Methods: Evaluation of mRNA levels was performed in paired non-inflamed and inflamed mucosal biopsies of newly diagnosed 50 Crohn's disease (CD) and 54 ulcerative colitis (UC) patients by quantitative real-time PCR analysis. Results: IL17A and IL17F expression levels were significantly increased in inflamed IBD mucosa. Inflamed CD ileal and UC mucosa showed increased IL23A, while only inflamed CD ileal samples showed increased TLR9 mRNA level. Correlation between analysed mRNAs levels and endoscopic and clinical disease activity were found in UC, but only with clinical activity in CD. Conclusion: Both CD and UC presented expression of Th17-associated genes. Nevertheless, expression profiles between different disease forms varies which should be taken into account for future research and therapeutics strategies.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Clinical Immunology
T1  - Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study
EP  - 95
SP  - 86
VL  - 197
DO  - 10.1016/j.clim.2018.09.001
ER  - 

@article{
author = "Dragasević, Sanja and Stanković, Biljana and Sokić-Milutinović, Aleksandra and Milosavljević, Tomica and Milovanović, Tamara and Lukić, Snežana and Srzentić Dražilov, Sanja and Klaassen, Kristel and Kotur, Nikola and Pavlović, Sonja and Popović, Dragan",
year = "2018",
abstract = "Background and aims: Mucosal gene expression have not been fully enlightened in inflammatory bowel disease (IBD). Aim of this study was to define IL23A, IL17A, IL17F and TLR9 expression in different IBD phenotypes. Methods: Evaluation of mRNA levels was performed in paired non-inflamed and inflamed mucosal biopsies of newly diagnosed 50 Crohn's disease (CD) and 54 ulcerative colitis (UC) patients by quantitative real-time PCR analysis. Results: IL17A and IL17F expression levels were significantly increased in inflamed IBD mucosa. Inflamed CD ileal and UC mucosa showed increased IL23A, while only inflamed CD ileal samples showed increased TLR9 mRNA level. Correlation between analysed mRNAs levels and endoscopic and clinical disease activity were found in UC, but only with clinical activity in CD. Conclusion: Both CD and UC presented expression of Th17-associated genes. Nevertheless, expression profiles between different disease forms varies which should be taken into account for future research and therapeutics strategies.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Clinical Immunology",
title = "Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study",
pages = "95-86",
volume = "197",
doi = "10.1016/j.clim.2018.09.001"
}

Dragasević, S., Stanković, B., Sokić-Milutinović, A., Milosavljević, T., Milovanović, T., Lukić, S., Srzentić Dražilov, S., Klaassen, K., Kotur, N., Pavlović, S.,& Popović, D.. (2018). Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study. in Clinical Immunology
Academic Press Inc Elsevier Science, San Diego., 197, 86-95.
https://doi.org/10.1016/j.clim.2018.09.001

Dragasević S, Stanković B, Sokić-Milutinović A, Milosavljević T, Milovanović T, Lukić S, Srzentić Dražilov S, Klaassen K, Kotur N, Pavlović S, Popović D. Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study. in Clinical Immunology. 2018;197:86-95.
doi:10.1016/j.clim.2018.09.001 .

Dragasević, Sanja, Stanković, Biljana, Sokić-Milutinović, Aleksandra, Milosavljević, Tomica, Milovanović, Tamara, Lukić, Snežana, Srzentić Dražilov, Sanja, Klaassen, Kristel, Kotur, Nikola, Pavlović, Sonja, Popović, Dragan, "Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study" in Clinical Immunology, 197 (2018):86-95,
https://doi.org/10.1016/j.clim.2018.09.001 . .

TY  - JOUR
AU  - Dragasević, Sanja
AU  - Stanković, Biljana
AU  - Milosavljević, Tomica
AU  - Sokić-Milutinović, Aleksandra
AU  - Lukić, Snežana
AU  - Alempijević, Tamara
AU  - Zukić, Branka
AU  - Kotur, Nikola
AU  - Nikčević, Gordana
AU  - Pavlović, Sonja
AU  - Popović, Dragan
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1063
AB  - Objectives The aim of the study was to evaluate associations between inflammatory bowel disease (IBD) presentation and variants in NOD2, TLR4, TNF-alpha, IL-6, IL-1 beta, and IL-RN genes in order to identify possible environmental factors that may affect IBD occurrence, investigate potential predictors for surgical treatment of IBD, and correlate the presence of granulomas in biopsy specimens with clinical characteristics of Crohn's disease (CD) patients. Patients and methods We genotyped 167 IBD patients using PCR-based methodology and tested for disease genotype-phenotype associations. Results In CD patients ileal localization of disease was more frequent in NOD2 variant carriers. Ileal CD was associated with IL-6 GC+CC genotypes, identifying C allele as a possible marker of increased risk for ileal CD. In CD patients a positive family history for IBD was related to earlier onset of disease, higher risk for CD-related surgery, and appendectomy. CD patients who are TLR4 299Gly carriers are at higher risk for surgery at onset of the disease compared with TLR4 299Asp variant carriers. The presence of granuloma in biopsy specimens was more frequent in patients in whom a diagnosis of CD was made during emergency surgery. Multivariate analysis showed that CD carriers of the 299Gly allele had a 4.6-fold higher risk for emergency surgery before CD diagnosis is established compared with noncarriers, suggesting an aggressive disease course. Granuloma in endoscopic biopsies is detected 5.4-fold more frequently in patients treated surgically at the time of diagnosis. Conclusion Genetic variants together with epidemiological and clinical data of IBD patients could potentially be used as predictors of the disease course.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - European Journal of Gastroenterology & Hepatology
T1  - Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease
EP  - 915
IS  - 8
SP  - 909
VL  - 29
DO  - 10.1097/MEG.0000000000000877
ER  - 

@article{
author = "Dragasević, Sanja and Stanković, Biljana and Milosavljević, Tomica and Sokić-Milutinović, Aleksandra and Lukić, Snežana and Alempijević, Tamara and Zukić, Branka and Kotur, Nikola and Nikčević, Gordana and Pavlović, Sonja and Popović, Dragan",
year = "2017",
abstract = "Objectives The aim of the study was to evaluate associations between inflammatory bowel disease (IBD) presentation and variants in NOD2, TLR4, TNF-alpha, IL-6, IL-1 beta, and IL-RN genes in order to identify possible environmental factors that may affect IBD occurrence, investigate potential predictors for surgical treatment of IBD, and correlate the presence of granulomas in biopsy specimens with clinical characteristics of Crohn's disease (CD) patients. Patients and methods We genotyped 167 IBD patients using PCR-based methodology and tested for disease genotype-phenotype associations. Results In CD patients ileal localization of disease was more frequent in NOD2 variant carriers. Ileal CD was associated with IL-6 GC+CC genotypes, identifying C allele as a possible marker of increased risk for ileal CD. In CD patients a positive family history for IBD was related to earlier onset of disease, higher risk for CD-related surgery, and appendectomy. CD patients who are TLR4 299Gly carriers are at higher risk for surgery at onset of the disease compared with TLR4 299Asp variant carriers. The presence of granuloma in biopsy specimens was more frequent in patients in whom a diagnosis of CD was made during emergency surgery. Multivariate analysis showed that CD carriers of the 299Gly allele had a 4.6-fold higher risk for emergency surgery before CD diagnosis is established compared with noncarriers, suggesting an aggressive disease course. Granuloma in endoscopic biopsies is detected 5.4-fold more frequently in patients treated surgically at the time of diagnosis. Conclusion Genetic variants together with epidemiological and clinical data of IBD patients could potentially be used as predictors of the disease course.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "European Journal of Gastroenterology & Hepatology",
title = "Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease",
pages = "915-909",
number = "8",
volume = "29",
doi = "10.1097/MEG.0000000000000877"
}

Dragasević, S., Stanković, B., Milosavljević, T., Sokić-Milutinović, A., Lukić, S., Alempijević, T., Zukić, B., Kotur, N., Nikčević, G., Pavlović, S.,& Popović, D.. (2017). Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease. in European Journal of Gastroenterology & Hepatology
Lippincott Williams & Wilkins, Philadelphia., 29(8), 909-915.
https://doi.org/10.1097/MEG.0000000000000877

Dragasević S, Stanković B, Milosavljević T, Sokić-Milutinović A, Lukić S, Alempijević T, Zukić B, Kotur N, Nikčević G, Pavlović S, Popović D. Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease. in European Journal of Gastroenterology & Hepatology. 2017;29(8):909-915.
doi:10.1097/MEG.0000000000000877 .

Dragasević, Sanja, Stanković, Biljana, Milosavljević, Tomica, Sokić-Milutinović, Aleksandra, Lukić, Snežana, Alempijević, Tamara, Zukić, Branka, Kotur, Nikola, Nikčević, Gordana, Pavlović, Sonja, Popović, Dragan, "Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease" in European Journal of Gastroenterology & Hepatology, 29, no. 8 (2017):909-915,
https://doi.org/10.1097/MEG.0000000000000877 . .

TY  - JOUR
AU  - Stanković, Biljana
AU  - Dragasević, Sanja
AU  - Popović, Dragan
AU  - Zukić, Branka
AU  - Kotur, Nikola
AU  - Sokić-Milutinović, Aleksandra
AU  - Alempijević, Tamara
AU  - Lukić, Snežana
AU  - Milosavljević, Tomica
AU  - Nikčević, Gordana
AU  - Pavlović, Sonja
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/810
AB  - OBJECTIVE: Research on inflammatory bowel disease (IBD) has highlighted genes involved in the regulation of inflammatory responses as contributors to disease pathogenesis. This study aimed to evaluate the associations between IBD and variations in NOD2, TLR4, TNF-alpha, IL-6, IL-1 beta and IL-1RN genes, and to use the genetic data obtained in predictive modeling. METHODS: A total of 167 IBD patients and 101 healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism. Using the genotype data attained as the input to various classification algorithms, IBD prediction models were designed. The area under the receiver operating characteristic curve (AUROC) was used to measure their performance. RESULTS: Significant associations were found between Crohn's disease (CD) and minor NOD2 variants, as well as TLR4 299Gly, TNF-alpha G-308A, IL-6 G-174C and IL-1RN VNTR A2 variants, while ulcerative colitis (UC) was associated only with IL-1RN VNTR A2 variants. CD and UC showed highly significant difference in the allelic distribution of TNF-alpha G-308A, where the A allele was found to be related to CD, and the G allele to UC. A combined effect of patients' gender and TLR4 variants was observed among CD patients. When all analyzed genotype and gender data were used, prediction performance achieved a maximum AUROC of 0.690 for CD and 0.601 for UC dataset. CONCLUSION: Variations in the genes involved in immune regulation are genetic factors of importance in IBD susceptibility that could potentially be used as predictors of disease development.
PB  - Wiley, Hoboken
T2  - Journal of Digestive Diseases
T1  - Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence
EP  - 733
IS  - 12
SP  - 723
VL  - 16
DO  - 10.1111/1751-2980.12281
ER  - 

@article{
author = "Stanković, Biljana and Dragasević, Sanja and Popović, Dragan and Zukić, Branka and Kotur, Nikola and Sokić-Milutinović, Aleksandra and Alempijević, Tamara and Lukić, Snežana and Milosavljević, Tomica and Nikčević, Gordana and Pavlović, Sonja",
year = "2015",
abstract = "OBJECTIVE: Research on inflammatory bowel disease (IBD) has highlighted genes involved in the regulation of inflammatory responses as contributors to disease pathogenesis. This study aimed to evaluate the associations between IBD and variations in NOD2, TLR4, TNF-alpha, IL-6, IL-1 beta and IL-1RN genes, and to use the genetic data obtained in predictive modeling. METHODS: A total of 167 IBD patients and 101 healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism. Using the genotype data attained as the input to various classification algorithms, IBD prediction models were designed. The area under the receiver operating characteristic curve (AUROC) was used to measure their performance. RESULTS: Significant associations were found between Crohn's disease (CD) and minor NOD2 variants, as well as TLR4 299Gly, TNF-alpha G-308A, IL-6 G-174C and IL-1RN VNTR A2 variants, while ulcerative colitis (UC) was associated only with IL-1RN VNTR A2 variants. CD and UC showed highly significant difference in the allelic distribution of TNF-alpha G-308A, where the A allele was found to be related to CD, and the G allele to UC. A combined effect of patients' gender and TLR4 variants was observed among CD patients. When all analyzed genotype and gender data were used, prediction performance achieved a maximum AUROC of 0.690 for CD and 0.601 for UC dataset. CONCLUSION: Variations in the genes involved in immune regulation are genetic factors of importance in IBD susceptibility that could potentially be used as predictors of disease development.",
publisher = "Wiley, Hoboken",
journal = "Journal of Digestive Diseases",
title = "Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence",
pages = "733-723",
number = "12",
volume = "16",
doi = "10.1111/1751-2980.12281"
}

Stanković, B., Dragasević, S., Popović, D., Zukić, B., Kotur, N., Sokić-Milutinović, A., Alempijević, T., Lukić, S., Milosavljević, T., Nikčević, G.,& Pavlović, S.. (2015). Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence. in Journal of Digestive Diseases
Wiley, Hoboken., 16(12), 723-733.
https://doi.org/10.1111/1751-2980.12281

Stanković B, Dragasević S, Popović D, Zukić B, Kotur N, Sokić-Milutinović A, Alempijević T, Lukić S, Milosavljević T, Nikčević G, Pavlović S. Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence. in Journal of Digestive Diseases. 2015;16(12):723-733.
doi:10.1111/1751-2980.12281 .

Stanković, Biljana, Dragasević, Sanja, Popović, Dragan, Zukić, Branka, Kotur, Nikola, Sokić-Milutinović, Aleksandra, Alempijević, Tamara, Lukić, Snežana, Milosavljević, Tomica, Nikčević, Gordana, Pavlović, Sonja, "Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence" in Journal of Digestive Diseases, 16, no. 12 (2015):723-733,
https://doi.org/10.1111/1751-2980.12281 . .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Dinić, Jelena
AU  - Radojković, Dragica
AU  - Lukić, Snežana
AU  - Popović, Dragan
AU  - Uglješić, Milenko
AU  - Knežević, Srboljub
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/517
AB  - Uvod: Mutacije u CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) genu mogu biti povezane sa različitim tipovima pankreasne patologije i da nose povišen rizik za oboljenja pankreasa. Najčešća mutacija u obolelih od cistične fibroze je F508del, dok je alel 5T povezan sa atipičnim oblicima cistične fibroze. Cilj rada: Cilj ove studije bio je da se utvrdi učestalost F508del mutacije i 5T alela u genu za transmembranski regulator provodljivosti u cističnoj fibrozi (CFTR ili Cystic Fibrosis Transmembrane Conductance Regulator) u grupama pacijenata sa hroničnim pankreatitisom i adenokarcinomom pankreasa, kao i da se istraži da li ove genske varijante predstavljaju faktore rizika za nastanak bolesti pankreasa. Metod rada: Istraživanje je obuhvatilo 50 pacijenata sa hroničnim pankreatitisom i 50 pacijenata sa adenokarcinomom pankreasa, kao i 124 zdrava ispitanika. Utvrđivanje prisustva F508del mutacije i detekcija 5T, 7T i 9T alela politimidinskog trakta vršeni su na DNK izolovanoj iz periferne krvi ispitanika pomoću metode PCR-om posredovane mesto- specifične mutageneze (PSM ili PCR-mediated site-directed mutagenesis). Rezultati: Učestalost F508del mutacije u hroničnom pankreatitisu (3,0%) bila je značajno povišena (p=0,032) u odnosu na učestalost u grupi zdravih ispitanika (0,4%), dok ostale razlike u učestalostima nisu bile statistički značajne. Zaključak: Rezultati ovog istraživanja ukazuju da F508del mutacija u CFTR genu predstavlja faktor rizika za razvoj hroničnog pankreatitisa.
AB  - Introduction: Mutations in the CFTR gene may be associated with various types of pancreatic pathology and result in higher risk of pancreatic disorders. While delta F508 is the most common mutation in cystic fibrosis patients, the allel 5T is associated with atypical forms of cystic fibrosis. Study aim: The aim of this study was to establish the frequencies of F508del mutation and 5T allele in the CFTR gene in patients with chronic pancreatitis and pancreatic cancer, as well as to investigate whether these genetic variants represent risk factors for pancreatic diseases. Study methods: The study has encompassed 50 patients with chronic pancreatitis and 50 patients with pancreatic adenocarcinoma, as well as 124 healthy individuals. The analysis of F508del mutation and alleles 5T, 7T and 9T of the polythymidine tract was performed on DNA extracted from periferal blood by PCR-mediated site-direted mutagenesis (PSM) method. Results: The frequency of F508del mutation in the group of patients with chronic pancreatitis (3.0%) was significantly increased (p=0.032) in comparison to the group of healthy individuals (0.4%), while other analyzed differences were not statistically significant. Conclusion: The results of this study indicate that F508del mutation in the CFTR gene respresents a risk factor for the development of chronic pancreatitis.
PB  - Udruženje hirurga Jugoslavije, Beograd
T2  - Acta chirurgica Iugoslavica
T1  - Zastupljenost F508del mutacije i 5T alela politimidinskog trakta u CFTR genu kod pacijenata sa hroničnim pankreatitisom i adenokarcinomom pankreasa
T1  - CFTR F508del mutation and 5T allele in patients with chronic pancreatitis and pancreatic adenocarcinoma
EP  - 47
IS  - 3
SP  - 43
VL  - 58
DO  - 10.2298/ACI1103043N
ER  - 

@article{
author = "Nikolić, Aleksandra and Dinić, Jelena and Radojković, Dragica and Lukić, Snežana and Popović, Dragan and Uglješić, Milenko and Knežević, Srboljub",
year = "2011",
abstract = "Uvod: Mutacije u CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) genu mogu biti povezane sa različitim tipovima pankreasne patologije i da nose povišen rizik za oboljenja pankreasa. Najčešća mutacija u obolelih od cistične fibroze je F508del, dok je alel 5T povezan sa atipičnim oblicima cistične fibroze. Cilj rada: Cilj ove studije bio je da se utvrdi učestalost F508del mutacije i 5T alela u genu za transmembranski regulator provodljivosti u cističnoj fibrozi (CFTR ili Cystic Fibrosis Transmembrane Conductance Regulator) u grupama pacijenata sa hroničnim pankreatitisom i adenokarcinomom pankreasa, kao i da se istraži da li ove genske varijante predstavljaju faktore rizika za nastanak bolesti pankreasa. Metod rada: Istraživanje je obuhvatilo 50 pacijenata sa hroničnim pankreatitisom i 50 pacijenata sa adenokarcinomom pankreasa, kao i 124 zdrava ispitanika. Utvrđivanje prisustva F508del mutacije i detekcija 5T, 7T i 9T alela politimidinskog trakta vršeni su na DNK izolovanoj iz periferne krvi ispitanika pomoću metode PCR-om posredovane mesto- specifične mutageneze (PSM ili PCR-mediated site-directed mutagenesis). Rezultati: Učestalost F508del mutacije u hroničnom pankreatitisu (3,0%) bila je značajno povišena (p=0,032) u odnosu na učestalost u grupi zdravih ispitanika (0,4%), dok ostale razlike u učestalostima nisu bile statistički značajne. Zaključak: Rezultati ovog istraživanja ukazuju da F508del mutacija u CFTR genu predstavlja faktor rizika za razvoj hroničnog pankreatitisa., Introduction: Mutations in the CFTR gene may be associated with various types of pancreatic pathology and result in higher risk of pancreatic disorders. While delta F508 is the most common mutation in cystic fibrosis patients, the allel 5T is associated with atypical forms of cystic fibrosis. Study aim: The aim of this study was to establish the frequencies of F508del mutation and 5T allele in the CFTR gene in patients with chronic pancreatitis and pancreatic cancer, as well as to investigate whether these genetic variants represent risk factors for pancreatic diseases. Study methods: The study has encompassed 50 patients with chronic pancreatitis and 50 patients with pancreatic adenocarcinoma, as well as 124 healthy individuals. The analysis of F508del mutation and alleles 5T, 7T and 9T of the polythymidine tract was performed on DNA extracted from periferal blood by PCR-mediated site-direted mutagenesis (PSM) method. Results: The frequency of F508del mutation in the group of patients with chronic pancreatitis (3.0%) was significantly increased (p=0.032) in comparison to the group of healthy individuals (0.4%), while other analyzed differences were not statistically significant. Conclusion: The results of this study indicate that F508del mutation in the CFTR gene respresents a risk factor for the development of chronic pancreatitis.",
publisher = "Udruženje hirurga Jugoslavije, Beograd",
journal = "Acta chirurgica Iugoslavica",
title = "Zastupljenost F508del mutacije i 5T alela politimidinskog trakta u CFTR genu kod pacijenata sa hroničnim pankreatitisom i adenokarcinomom pankreasa, CFTR F508del mutation and 5T allele in patients with chronic pancreatitis and pancreatic adenocarcinoma",
pages = "47-43",
number = "3",
volume = "58",
doi = "10.2298/ACI1103043N"
}

Nikolić, A., Dinić, J., Radojković, D., Lukić, S., Popović, D., Uglješić, M.,& Knežević, S.. (2011). Zastupljenost F508del mutacije i 5T alela politimidinskog trakta u CFTR genu kod pacijenata sa hroničnim pankreatitisom i adenokarcinomom pankreasa. in Acta chirurgica Iugoslavica
Udruženje hirurga Jugoslavije, Beograd., 58(3), 43-47.
https://doi.org/10.2298/ACI1103043N

Nikolić A, Dinić J, Radojković D, Lukić S, Popović D, Uglješić M, Knežević S. Zastupljenost F508del mutacije i 5T alela politimidinskog trakta u CFTR genu kod pacijenata sa hroničnim pankreatitisom i adenokarcinomom pankreasa. in Acta chirurgica Iugoslavica. 2011;58(3):43-47.
doi:10.2298/ACI1103043N .

Nikolić, Aleksandra, Dinić, Jelena, Radojković, Dragica, Lukić, Snežana, Popović, Dragan, Uglješić, Milenko, Knežević, Srboljub, "Zastupljenost F508del mutacije i 5T alela politimidinskog trakta u CFTR genu kod pacijenata sa hroničnim pankreatitisom i adenokarcinomom pankreasa" in Acta chirurgica Iugoslavica, 58, no. 3 (2011):43-47,
https://doi.org/10.2298/ACI1103043N . .

TY  - JOUR
AU  - Lukić, Snežana
AU  - Nikolić, Aleksandra
AU  - Alempijević, Tamara
AU  - Popović, Dragan
AU  - Sokic Milutinović, Aleksandra
AU  - Ugljesić, Milenko
AU  - Knezević, Srbislav
AU  - Milicić, Biljana
AU  - Dinić, Dragica
AU  - Radojković, Dragica
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/489
AB  - The purpose of this study was to determine the frequency of angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and to investigate its role as a potential risk factor in patients with chronic pancreatitis and pancreatic cancer. Deletion polymorphism of the 287-bp fragment of intron 16 of the ACE gene results in higher levels of circulating enzyme and therefore may represent a risk factor for disease development. The study included 55 patients with chronic pancreatitis, 45 patients with pancreatic cancer and 128 healthy subjects. The presence of I and D variants in the ACE gene was analyzed by a polymerase chain reaction (PCR) method. Distribution of ACE ID genotypes was analyzed by means of logistic regression. When chronic pancreatitis and pancreatic cancer groups were compared in the univariate analysis, the following factors were identified as statistically significant predictors of pancreatic disease: age, gender, smoking, fat intake, ACE II genotype and ACE DD genotype. However, in the multivariate analysis, only age, gender and smoking were singled out as predictors for the occurrence of pancreatic disease. Our findings indicate that the ACE I/D polymorphism could play a role in the development of chronic pancreatitis and pancreatic cancer through interaction with other genetic and environmental factors.
PB  - Karger, Basel
T2  - Digestive Surgery
T1  - Angiotensin-Converting Enzyme Gene Insertion/Deletion Polymorphism in Patients with Chronic Pancreatitis and Pancreatic Cancer
EP  - 262
IS  - 4
SP  - 258
VL  - 28
DO  - 10.1159/000328666
ER  - 

@article{
author = "Lukić, Snežana and Nikolić, Aleksandra and Alempijević, Tamara and Popović, Dragan and Sokic Milutinović, Aleksandra and Ugljesić, Milenko and Knezević, Srbislav and Milicić, Biljana and Dinić, Dragica and Radojković, Dragica",
year = "2011",
abstract = "The purpose of this study was to determine the frequency of angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and to investigate its role as a potential risk factor in patients with chronic pancreatitis and pancreatic cancer. Deletion polymorphism of the 287-bp fragment of intron 16 of the ACE gene results in higher levels of circulating enzyme and therefore may represent a risk factor for disease development. The study included 55 patients with chronic pancreatitis, 45 patients with pancreatic cancer and 128 healthy subjects. The presence of I and D variants in the ACE gene was analyzed by a polymerase chain reaction (PCR) method. Distribution of ACE ID genotypes was analyzed by means of logistic regression. When chronic pancreatitis and pancreatic cancer groups were compared in the univariate analysis, the following factors were identified as statistically significant predictors of pancreatic disease: age, gender, smoking, fat intake, ACE II genotype and ACE DD genotype. However, in the multivariate analysis, only age, gender and smoking were singled out as predictors for the occurrence of pancreatic disease. Our findings indicate that the ACE I/D polymorphism could play a role in the development of chronic pancreatitis and pancreatic cancer through interaction with other genetic and environmental factors.",
publisher = "Karger, Basel",
journal = "Digestive Surgery",
title = "Angiotensin-Converting Enzyme Gene Insertion/Deletion Polymorphism in Patients with Chronic Pancreatitis and Pancreatic Cancer",
pages = "262-258",
number = "4",
volume = "28",
doi = "10.1159/000328666"
}

Lukić, S., Nikolić, A., Alempijević, T., Popović, D., Sokic Milutinović, A., Ugljesić, M., Knezević, S., Milicić, B., Dinić, D.,& Radojković, D.. (2011). Angiotensin-Converting Enzyme Gene Insertion/Deletion Polymorphism in Patients with Chronic Pancreatitis and Pancreatic Cancer. in Digestive Surgery
Karger, Basel., 28(4), 258-262.
https://doi.org/10.1159/000328666

Lukić S, Nikolić A, Alempijević T, Popović D, Sokic Milutinović A, Ugljesić M, Knezević S, Milicić B, Dinić D, Radojković D. Angiotensin-Converting Enzyme Gene Insertion/Deletion Polymorphism in Patients with Chronic Pancreatitis and Pancreatic Cancer. in Digestive Surgery. 2011;28(4):258-262.
doi:10.1159/000328666 .

Lukić, Snežana, Nikolić, Aleksandra, Alempijević, Tamara, Popović, Dragan, Sokic Milutinović, Aleksandra, Ugljesić, Milenko, Knezević, Srbislav, Milicić, Biljana, Dinić, Dragica, Radojković, Dragica, "Angiotensin-Converting Enzyme Gene Insertion/Deletion Polymorphism in Patients with Chronic Pancreatitis and Pancreatic Cancer" in Digestive Surgery, 28, no. 4 (2011):258-262,
https://doi.org/10.1159/000328666 . .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Divac Rankov, Aleksandra
AU  - Stanković, Marija
AU  - Dinić, Jelena
AU  - Lukić, Snežana
AU  - Anđelić-Jelić, Marina
AU  - Popović, Dragan
AU  - Radojković, Dragica
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/443
AB  - Jedan od osnovnih izazova u proučavanju patologije bolesti pankreasa predstavlja dalje razjašnjavanje uloge proteaza i antiproteaza, zbog toga što poremećena ravnoteža između njih može dovesti do oštećenja pankreasa. Alfa 1-antitripsin (AAT) je jedan od najvažnijih inhibitora proteolitičkih enzima u serumu, među kojima su i enzimi pankreasa: tripsin, himotripsin i elastaza. Pretpostavlja se da mutacije u AAT genu mogu da utiču na pojavu i razvoj bolesti pankreasa. Prisustvo najčešćih mutacija u AAT genu, označenih kao Z i S, analizirano je u 160 pacijenata sa bolestima pankreasa (50 pacijenata sa kancerom pankreasa, 50 pacijenata sa hroničnim pankreatitisom i 60 pacijenata sa dijabetesom tipa 2) i u 129 zdravih osoba. Prisustvo mutacija detektovano je analizom dužina restrikcionih fragmenata. Jedan pacijent sa kancerom pankreasa je bio heterozigotni nosilac Z mutacije, kao i jedan pacijent sa dijabetesom tipa 2. Jedan pacijent sa hroničnim pankreatitisom je bio heterozigotni nosilac S mutacije. Dve najčešće mutacije u AAT genu su bile statistički značajno učestalije kod pacijenata sa bolestima pankreasa (3 / 160 pacijenata, alelska frekvencija 0,9%) nego u kontrolnoj grupi (1 / 129 osoba, alelska frekvencija 0,4%). Rezultati ove studije, koje ukazuju na moguću povezanost Z i S mutacija sa umerenim povećanjem rizika za razvoj bolesti pankreasa.
AB  - One of the key points in research of pancreatic disease pathology is further elucidation of the role of proteases and antiproteases, since their imbalance can lead to pancreatic injury. Alpha 1-antitrypsin (AAT) is one of the most important serum inhibitors of proteolytic enzymes, including pancreatic enzymes trypsin, chymotrypsin and elastase. It is speculated that mutations in the AAT gene may influence the onset and the development of pancreatic disease. The presence of the most common AAT mutations Z and S was analyzed in 160 patients with pancreatic diseases (50 patients with pancreatic cancer, 50 patients with chronic pancreatitis and 60 patients with type 2 diabetes mellitus) and 129 healthy individuals by PCR-mediated site-directed mutagenesis (PSM) method. One patient with pancreatic cancer was found to be a carrier of Z mutation, as well as one patient with type 2 diabetes mellitus. One patient with chronic pancreatitis was found to be a carrier of S mutation. The common AAT mutations were statistically significantly over-represented in patients with pancreatic diseases (3 of 160 patients, allelic frequency 0.9%) than in the control group (1 of 129 individuals, allelic frequency 0.4%). The results of this study, requiring confirmation, suggest that common AAT mutations Z and S may be associated with a modest increase in susceptibility to the development of pancreatic disease.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Učestalost S i Z mutacija u genu za alfa 1-antitripsin kod pacijenata sa bolestima pankreasa
T1  - Prevalence of S and Z alpha 1-antitrypsin mutations in patients with pancreatic diseases in Serbian population
EP  - 385
IS  - 2
SP  - 377
VL  - 42
DO  - 10.2298/GENSR1002377N
ER  - 

@article{
author = "Nikolić, Aleksandra and Divac Rankov, Aleksandra and Stanković, Marija and Dinić, Jelena and Lukić, Snežana and Anđelić-Jelić, Marina and Popović, Dragan and Radojković, Dragica",
year = "2010",
abstract = "Jedan od osnovnih izazova u proučavanju patologije bolesti pankreasa predstavlja dalje razjašnjavanje uloge proteaza i antiproteaza, zbog toga što poremećena ravnoteža između njih može dovesti do oštećenja pankreasa. Alfa 1-antitripsin (AAT) je jedan od najvažnijih inhibitora proteolitičkih enzima u serumu, među kojima su i enzimi pankreasa: tripsin, himotripsin i elastaza. Pretpostavlja se da mutacije u AAT genu mogu da utiču na pojavu i razvoj bolesti pankreasa. Prisustvo najčešćih mutacija u AAT genu, označenih kao Z i S, analizirano je u 160 pacijenata sa bolestima pankreasa (50 pacijenata sa kancerom pankreasa, 50 pacijenata sa hroničnim pankreatitisom i 60 pacijenata sa dijabetesom tipa 2) i u 129 zdravih osoba. Prisustvo mutacija detektovano je analizom dužina restrikcionih fragmenata. Jedan pacijent sa kancerom pankreasa je bio heterozigotni nosilac Z mutacije, kao i jedan pacijent sa dijabetesom tipa 2. Jedan pacijent sa hroničnim pankreatitisom je bio heterozigotni nosilac S mutacije. Dve najčešće mutacije u AAT genu su bile statistički značajno učestalije kod pacijenata sa bolestima pankreasa (3 / 160 pacijenata, alelska frekvencija 0,9%) nego u kontrolnoj grupi (1 / 129 osoba, alelska frekvencija 0,4%). Rezultati ove studije, koje ukazuju na moguću povezanost Z i S mutacija sa umerenim povećanjem rizika za razvoj bolesti pankreasa., One of the key points in research of pancreatic disease pathology is further elucidation of the role of proteases and antiproteases, since their imbalance can lead to pancreatic injury. Alpha 1-antitrypsin (AAT) is one of the most important serum inhibitors of proteolytic enzymes, including pancreatic enzymes trypsin, chymotrypsin and elastase. It is speculated that mutations in the AAT gene may influence the onset and the development of pancreatic disease. The presence of the most common AAT mutations Z and S was analyzed in 160 patients with pancreatic diseases (50 patients with pancreatic cancer, 50 patients with chronic pancreatitis and 60 patients with type 2 diabetes mellitus) and 129 healthy individuals by PCR-mediated site-directed mutagenesis (PSM) method. One patient with pancreatic cancer was found to be a carrier of Z mutation, as well as one patient with type 2 diabetes mellitus. One patient with chronic pancreatitis was found to be a carrier of S mutation. The common AAT mutations were statistically significantly over-represented in patients with pancreatic diseases (3 of 160 patients, allelic frequency 0.9%) than in the control group (1 of 129 individuals, allelic frequency 0.4%). The results of this study, requiring confirmation, suggest that common AAT mutations Z and S may be associated with a modest increase in susceptibility to the development of pancreatic disease.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Učestalost S i Z mutacija u genu za alfa 1-antitripsin kod pacijenata sa bolestima pankreasa, Prevalence of S and Z alpha 1-antitrypsin mutations in patients with pancreatic diseases in Serbian population",
pages = "385-377",
number = "2",
volume = "42",
doi = "10.2298/GENSR1002377N"
}

Nikolić, A., Divac Rankov, A., Stanković, M., Dinić, J., Lukić, S., Anđelić-Jelić, M., Popović, D.,& Radojković, D.. (2010). Učestalost S i Z mutacija u genu za alfa 1-antitripsin kod pacijenata sa bolestima pankreasa. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 42(2), 377-385.
https://doi.org/10.2298/GENSR1002377N

Nikolić A, Divac Rankov A, Stanković M, Dinić J, Lukić S, Anđelić-Jelić M, Popović D, Radojković D. Učestalost S i Z mutacija u genu za alfa 1-antitripsin kod pacijenata sa bolestima pankreasa. in Genetika-Belgrade. 2010;42(2):377-385.
doi:10.2298/GENSR1002377N .

Nikolić, Aleksandra, Divac Rankov, Aleksandra, Stanković, Marija, Dinić, Jelena, Lukić, Snežana, Anđelić-Jelić, Marina, Popović, Dragan, Radojković, Dragica, "Učestalost S i Z mutacija u genu za alfa 1-antitripsin kod pacijenata sa bolestima pankreasa" in Genetika-Belgrade, 42, no. 2 (2010):377-385,
https://doi.org/10.2298/GENSR1002377N . .

TY  - JOUR
AU  - Nisević, Ivan
AU  - Dinić, Jelena
AU  - Nikolić, Aleksandra
AU  - Đorđević, Valentina
AU  - Lukić, Snežana
AU  - Ugljesić, Milenko
AU  - Anđelić-Jelić, Marina
AU  - Petrović-Stanojević, Nataša
AU  - Radojković, Dragica
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/325
AB  - Chronic pancreatitis and pancreatic adenocarcinoma are extensively studied as common and potentially lethal disorders. However, their Causes and genetic background in most cases remain unclear. The C677T polymorphism in 5',10'-methylenetetrahydrofolate reductase (MTHFR) gene may modulate the risk of pancreatic disorders. In this Study, we tested whether MTHFR C677T polymorphism is associated with chronic pancreatitis and pancreatic adenocarcinoma in the Serbian population. DNA was extracted from blood samples of 51 chronic pancreatitis patients, 21 pancreatic adenocarcinoma patients, and a control group consisting of 50 healthy smokers. The MTHFR C677T polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Although, no statistically significant differences were observed in the distribution of MTHFR genotype or allele frequencies between patients and control groups, the results showed an increased frequency of homozygotes for MTHFR C677T polymorphisrn in chronic pancreatitis patients ( 14%) and a decreased frequency in pancreatic adenocarcinoma patients (5%) in comparison to the control group (8%). We speculate that the MTHFR C677T polymorphism Could act as a possible risk factor for chronic pancreatitis and a possible protective factor in pancreatic adenocarcinoma. This observation needs further investigation in prospective Studies on a larger number of patients, in which the effect of other genetic and environmental factors should also be taken into consideration.
PB  - Wiley, Hoboken
T2  - Cell Biochemistry and Function
T1  - MTHFR C677T polymorphism in chronic pancreatitis and pancreatic adenocarcinoma
EP  - 663
IS  - 6
SP  - 659
VL  - 26
DO  - 10.1002/cbf.1487
ER  - 

@article{
author = "Nisević, Ivan and Dinić, Jelena and Nikolić, Aleksandra and Đorđević, Valentina and Lukić, Snežana and Ugljesić, Milenko and Anđelić-Jelić, Marina and Petrović-Stanojević, Nataša and Radojković, Dragica",
year = "2008",
abstract = "Chronic pancreatitis and pancreatic adenocarcinoma are extensively studied as common and potentially lethal disorders. However, their Causes and genetic background in most cases remain unclear. The C677T polymorphism in 5',10'-methylenetetrahydrofolate reductase (MTHFR) gene may modulate the risk of pancreatic disorders. In this Study, we tested whether MTHFR C677T polymorphism is associated with chronic pancreatitis and pancreatic adenocarcinoma in the Serbian population. DNA was extracted from blood samples of 51 chronic pancreatitis patients, 21 pancreatic adenocarcinoma patients, and a control group consisting of 50 healthy smokers. The MTHFR C677T polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Although, no statistically significant differences were observed in the distribution of MTHFR genotype or allele frequencies between patients and control groups, the results showed an increased frequency of homozygotes for MTHFR C677T polymorphisrn in chronic pancreatitis patients ( 14%) and a decreased frequency in pancreatic adenocarcinoma patients (5%) in comparison to the control group (8%). We speculate that the MTHFR C677T polymorphism Could act as a possible risk factor for chronic pancreatitis and a possible protective factor in pancreatic adenocarcinoma. This observation needs further investigation in prospective Studies on a larger number of patients, in which the effect of other genetic and environmental factors should also be taken into consideration.",
publisher = "Wiley, Hoboken",
journal = "Cell Biochemistry and Function",
title = "MTHFR C677T polymorphism in chronic pancreatitis and pancreatic adenocarcinoma",
pages = "663-659",
number = "6",
volume = "26",
doi = "10.1002/cbf.1487"
}

Nisević, I., Dinić, J., Nikolić, A., Đorđević, V., Lukić, S., Ugljesić, M., Anđelić-Jelić, M., Petrović-Stanojević, N.,& Radojković, D.. (2008). MTHFR C677T polymorphism in chronic pancreatitis and pancreatic adenocarcinoma. in Cell Biochemistry and Function
Wiley, Hoboken., 26(6), 659-663.
https://doi.org/10.1002/cbf.1487

Nisević I, Dinić J, Nikolić A, Đorđević V, Lukić S, Ugljesić M, Anđelić-Jelić M, Petrović-Stanojević N, Radojković D. MTHFR C677T polymorphism in chronic pancreatitis and pancreatic adenocarcinoma. in Cell Biochemistry and Function. 2008;26(6):659-663.
doi:10.1002/cbf.1487 .

Nisević, Ivan, Dinić, Jelena, Nikolić, Aleksandra, Đorđević, Valentina, Lukić, Snežana, Ugljesić, Milenko, Anđelić-Jelić, Marina, Petrović-Stanojević, Nataša, Radojković, Dragica, "MTHFR C677T polymorphism in chronic pancreatitis and pancreatic adenocarcinoma" in Cell Biochemistry and Function, 26, no. 6 (2008):659-663,
https://doi.org/10.1002/cbf.1487 . .