@conference{
author = "Nešić, Sofija and Divac Rankov, Aleksandra and Spasovski, Vesna and Kosanović, Maja and Nikolić, Dragana",
year = "2023",
abstract = "Extracellular vesicles (EVs) are recognized as important mediators of intercellular
communication in both eukaryotes and prokaryotes. These lipid membrane –
coated spherical nanoparticles carry proteins, nucleic acids and other cellular
products, and facilitate exchange of these biomolecules among cells within an
organism, but also between cells of different organisms, belonging to different
species and even kingdoms. Outer membrane vesicles (OMVs), EVs produced
by Gram-negative bacteria, are a significant mediator of microbial communication,
involved in biofilm formation, virulence, and modulation of host immunity.
OMVs of both pathogenic and plant beneficial bacteria have been shown
to elicit plant immune responses. Investigations on the modes of OMV-plant
cells interactions are still in their infancy, but gain rising attention. Aiming to
monitor the interaction between OMVs of Paraburkholderia phytofirmans PsJN,
a plant growth promoting bacteria, and Arabidopsis thaliana roots, we isolated
OMVs from bacterial culture in mineral medium, using an ion-exchange chromatography
system. Isolated OMVs were labeled with lipid binding fluorescent
dye Vybrant™ DiD and unbound dye was removed by washing vesicles on ultrafiltration
columns. The same dye concentration in phosphate buffer saline,
equivalently washed, was used as a control. A. thaliana roots, grown on Murashige
and Skoog medium, were incubated with DiD-OMVs or control dye/buffer
mixture, washed and observed under confocal laser scanning microscope. Red
signals were observed in root hairs and epidermis in DiD-OMV treated plants,
while in control-treated roots the same signals were missing. The results indicate
direct contact of bacterial vesicles with epidermis and root hairs, which are
indispensable for nutrient acquisition and plant-microbe interactions in rhizosphere.
Further investigation will address the questions of the nature of OMVplant
cell interaction, including potential delivery of OMVs cargo into host plant
cells. Considering that OMVs are increasingly recognized as promising tools in
biomedicine, exploring their potential for agronomical applications would be
highly appreciated.",
publisher = "Belgrade : Faculty of Biology",
journal = "ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture",
title = "Outer membrane vesicles of plant beneficial bacterial strain
Paraburkholderia phytofirmans PsJN make a contact with
Arabidopsis thaliana roots",
pages = "89-89",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2157"
}
