TY  - JOUR
AU  - Rajković, Katarina
AU  - Drobac, Milica
AU  - Milić, Petar
AU  - Vučić, Vesna
AU  - Arsić, Aleksandra
AU  - Perić, Mirjana
AU  - Radunović, Milena
AU  - Jeremić, Sanja
AU  - Arsenijević, Jelena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1805
AB  - Juglans nigra (Black walnut) is a source of health-promoting
biologically active compounds used in traditional medicine. The investigation of
bioactive compounds in black walnut could lead to its broader application, as
well as application of its by-products. Therefore, this study aimed to characterize
J. nigra nut and green husk based on chemical analysis of their petroleum ether
and ethanol extracts obtained by ultrasonic and reflux extraction methods,
respectively. Different extract fractions were tested for their antimicrobial
activities using Gram-negative bacteria (Escherichia coli, Pseudomonas
aeruginosa), Gram-positive bacteria (Enterococcus faecalis Staphylococcus
aureus) and yeast (reference strain and clinical isolates of Candida albicans).
Ethanol extracts analysis, performed by High Performance Liquid
Chromatography (HPLC), singled out the ellagic acid as the most dominant
compound in nut (55.0 ± 1.3 × 10-3 kg m-3
) and green husk (114.1 ± 0.5× 10-3 kg
m-3
) extracts. Non-polar compounds were evaluated using Gas Chromatography
(GC) analysis of petroleum ether extracts. Juglans nigra nut and green husk
contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid
(C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic
(C18:1n-9), and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids,
linoleic (C18:2n-6), γ-linolenic (C18:3n-6), and α-linolenic (C18:3n-3) acids.
Ethanol extracts of both J. nigra nut and green husk showed antimicrobial
activity against C. albicans which is the most common cause of yeast infections.
AB  - Juglans nigra (црни орах) као извор биолошки активних једињења користе се у
традиционалној медицини. Истраживање биоактивних једињења присутних у црном ораху
довело би до шире примене његових производа. Зато ова студија има за циљ да
окарактерише орашаст плод и зелену љуску Ј. nigra на основу хемијске анализе њихових
петролетарских и етанолних екстраката, добијених ултразвучним и рефлукс методама
екстракције. Антимикробна активност различитих фракција екстракта је тестирана
коришћењем Грам-негативних бактерија (Escherichia coli, Pseudomonas aeruginosa), Грамозитивних бактерија (Enterococcus faecalis Staphylococcus aureus) и квасница (референтни
сој и клинички изолати Candida albicans). Анализа течном хроматографијом високих
перформанси, издвојила је елагинску киселину као најдоминантније једињење у етанолним
екстрактима орашастог плода (55,0 ± 1,3 × 10-3 kg m-3) и зелене љуске (114,1 ± 0,5 × 10-3
g m-3). Неполарна једињења су процењена применом гасне хроматографске анализе
петролетарских екстракта. Орашаст плод и зелена љуска садрже две засићене масне
киселине, палмитинску (C16:0) и стеаринску киселину (C18:0), затим мононезасићене
масне киселине, палмитолеинску (C16:1n-7), олеинску (C18:1n-9), и вакценску киселину
(C18:1n-7), као и полинезасићене масне киселине, линолну (C18:2n-6), γ-линоленску
(C18:3n-6) и α-линоленску (C18: 3n-3) киселину. Етанолни екстракти орашастог плода и
зелене љуске показали су антимикробну активност нa сојевима C. albicans која је најчешћи
узрочник гљивичних инфекција.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk
T1  - Hemijska karakterizacija i antimikrobna aktivnost orašastog ploda i zelene ljuske Juglans nigra l.
T1  - Xемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra l.
DO  - doi.org/10.2298/JSC230210024R
ER  - 

@article{
author = "Rajković, Katarina and Drobac, Milica and Milić, Petar and Vučić, Vesna and Arsić, Aleksandra and Perić, Mirjana and Radunović, Milena and Jeremić, Sanja and Arsenijević, Jelena",
year = "2023",
abstract = "Juglans nigra (Black walnut) is a source of health-promoting
biologically active compounds used in traditional medicine. The investigation of
bioactive compounds in black walnut could lead to its broader application, as
well as application of its by-products. Therefore, this study aimed to characterize
J. nigra nut and green husk based on chemical analysis of their petroleum ether
and ethanol extracts obtained by ultrasonic and reflux extraction methods,
respectively. Different extract fractions were tested for their antimicrobial
activities using Gram-negative bacteria (Escherichia coli, Pseudomonas
aeruginosa), Gram-positive bacteria (Enterococcus faecalis Staphylococcus
aureus) and yeast (reference strain and clinical isolates of Candida albicans).
Ethanol extracts analysis, performed by High Performance Liquid
Chromatography (HPLC), singled out the ellagic acid as the most dominant
compound in nut (55.0 ± 1.3 × 10-3 kg m-3
) and green husk (114.1 ± 0.5× 10-3 kg
m-3
) extracts. Non-polar compounds were evaluated using Gas Chromatography
(GC) analysis of petroleum ether extracts. Juglans nigra nut and green husk
contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid
(C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic
(C18:1n-9), and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids,
linoleic (C18:2n-6), γ-linolenic (C18:3n-6), and α-linolenic (C18:3n-3) acids.
Ethanol extracts of both J. nigra nut and green husk showed antimicrobial
activity against C. albicans which is the most common cause of yeast infections., Juglans nigra (црни орах) као извор биолошки активних једињења користе се у
традиционалној медицини. Истраживање биоактивних једињења присутних у црном ораху
довело би до шире примене његових производа. Зато ова студија има за циљ да
окарактерише орашаст плод и зелену љуску Ј. nigra на основу хемијске анализе њихових
петролетарских и етанолних екстраката, добијених ултразвучним и рефлукс методама
екстракције. Антимикробна активност различитих фракција екстракта је тестирана
коришћењем Грам-негативних бактерија (Escherichia coli, Pseudomonas aeruginosa), Грамозитивних бактерија (Enterococcus faecalis Staphylococcus aureus) и квасница (референтни
сој и клинички изолати Candida albicans). Анализа течном хроматографијом високих
перформанси, издвојила је елагинску киселину као најдоминантније једињење у етанолним
екстрактима орашастог плода (55,0 ± 1,3 × 10-3 kg m-3) и зелене љуске (114,1 ± 0,5 × 10-3
g m-3). Неполарна једињења су процењена применом гасне хроматографске анализе
петролетарских екстракта. Орашаст плод и зелена љуска садрже две засићене масне
киселине, палмитинску (C16:0) и стеаринску киселину (C18:0), затим мононезасићене
масне киселине, палмитолеинску (C16:1n-7), олеинску (C18:1n-9), и вакценску киселину
(C18:1n-7), као и полинезасићене масне киселине, линолну (C18:2n-6), γ-линоленску
(C18:3n-6) и α-линоленску (C18: 3n-3) киселину. Етанолни екстракти орашастог плода и
зелене љуске показали су антимикробну активност нa сојевима C. albicans која је најчешћи
узрочник гљивичних инфекција.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk, Hemijska karakterizacija i antimikrobna aktivnost orašastog ploda i zelene ljuske Juglans nigra l., Xемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra l.",
doi = "doi.org/10.2298/JSC230210024R"
}

Rajković, K., Drobac, M., Milić, P., Vučić, V., Arsić, A., Perić, M., Radunović, M., Jeremić, S.,& Arsenijević, J.. (2023). Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society..
https://doi.org/doi.org/10.2298/JSC230210024R

Rajković K, Drobac M, Milić P, Vučić V, Arsić A, Perić M, Radunović M, Jeremić S, Arsenijević J. Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society. 2023;.
doi:doi.org/10.2298/JSC230210024R .

Rajković, Katarina, Drobac, Milica, Milić, Petar, Vučić, Vesna, Arsić, Aleksandra, Perić, Mirjana, Radunović, Milena, Jeremić, Sanja, Arsenijević, Jelena, "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk" in Journal of the Serbian Chemical Society (2023),
https://doi.org/doi.org/10.2298/JSC230210024R . .

TY  - JOUR
AU  - Rajković, Katarina M.
AU  - Vasić, Marijana
AU  - Drobac, Milica
AU  - Mutić, Jelena
AU  - Jeremić, Sanja
AU  - Simić, Valentina
AU  - Stanković, Jovan
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1378
AB  - The extraction yield of Juglans nigra L. leaves was assessed at different ethanol concentrations (0-96% (v/v)) and solvent-to-solid ratios (5-20 kg kg(-1)). The response surface methodology (RSM) and artificial neural network with genetic algorithms (ANN-GA) were developed to optimize the extraction variables. The RSM and ANN-GA models determined 50% (v/v) ethanol concentration and 20 kg kg(-1) solvent-to-solid ratio as optimal conditions, ensuring an extraction yield of 27.69 and 27.19 g 100 g(-1) of dry leaves. The phenolic compounds in optimal extract were quantified: 3-O-caffeoylquinic acid (2.27 mg g(-1)of dry leaves), quercetin-3-O-galactoside (10.99 mg g(-1) of dry leaves) and quercetin 3 0 rhamnoside (15.07 mg g(-1)of dry leaves) using high-performance liquid chromatography (HPLC). The minerals in optimal extract were quantified: macro-elements (the relative order by content was: K  gt  Mg  gt  Ca) using inductively coupled plasma optical emission spectrometry (ICP-OES) and micro-elements (the relative order by content was: Zn  gt  Rb  gt  Mn  gt  I gt Sr  gt  Ni  gt  Cu  gt  Co  gt  V  gt  Ag  gt  Se) using inductively coupled plasma mass spectrometry (ICP-MS). The extraction coefficients for minerals were determined and were highest for K (64.3%) and I (53.5%). Optimization of extraction process resulted in high extraction yield from J. nigra leaves and optimal extract containing different phytochemical compounds.
PB  - Elsevier, Amsterdam
T2  - Chemical Engineering Research & Design
T1  - Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves
EP  - 33
SP  - 25
VL  - 157
DO  - 10.1016/j.cherd.2020.03.002
ER  - 

@article{
author = "Rajković, Katarina M. and Vasić, Marijana and Drobac, Milica and Mutić, Jelena and Jeremić, Sanja and Simić, Valentina and Stanković, Jovan",
year = "2020",
abstract = "The extraction yield of Juglans nigra L. leaves was assessed at different ethanol concentrations (0-96% (v/v)) and solvent-to-solid ratios (5-20 kg kg(-1)). The response surface methodology (RSM) and artificial neural network with genetic algorithms (ANN-GA) were developed to optimize the extraction variables. The RSM and ANN-GA models determined 50% (v/v) ethanol concentration and 20 kg kg(-1) solvent-to-solid ratio as optimal conditions, ensuring an extraction yield of 27.69 and 27.19 g 100 g(-1) of dry leaves. The phenolic compounds in optimal extract were quantified: 3-O-caffeoylquinic acid (2.27 mg g(-1)of dry leaves), quercetin-3-O-galactoside (10.99 mg g(-1) of dry leaves) and quercetin 3 0 rhamnoside (15.07 mg g(-1)of dry leaves) using high-performance liquid chromatography (HPLC). The minerals in optimal extract were quantified: macro-elements (the relative order by content was: K  gt  Mg  gt  Ca) using inductively coupled plasma optical emission spectrometry (ICP-OES) and micro-elements (the relative order by content was: Zn  gt  Rb  gt  Mn  gt  I gt Sr  gt  Ni  gt  Cu  gt  Co  gt  V  gt  Ag  gt  Se) using inductively coupled plasma mass spectrometry (ICP-MS). The extraction coefficients for minerals were determined and were highest for K (64.3%) and I (53.5%). Optimization of extraction process resulted in high extraction yield from J. nigra leaves and optimal extract containing different phytochemical compounds.",
publisher = "Elsevier, Amsterdam",
journal = "Chemical Engineering Research & Design",
title = "Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves",
pages = "33-25",
volume = "157",
doi = "10.1016/j.cherd.2020.03.002"
}

Rajković, K. M., Vasić, M., Drobac, M., Mutić, J., Jeremić, S., Simić, V.,& Stanković, J.. (2020). Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves. in Chemical Engineering Research & Design
Elsevier, Amsterdam., 157, 25-33.
https://doi.org/10.1016/j.cherd.2020.03.002

Rajković KM, Vasić M, Drobac M, Mutić J, Jeremić S, Simić V, Stanković J. Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves. in Chemical Engineering Research & Design. 2020;157:25-33.
doi:10.1016/j.cherd.2020.03.002 .

Rajković, Katarina M., Vasić, Marijana, Drobac, Milica, Mutić, Jelena, Jeremić, Sanja, Simić, Valentina, Stanković, Jovan, "Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves" in Chemical Engineering Research & Design, 157 (2020):25-33,
https://doi.org/10.1016/j.cherd.2020.03.002 . .