TY  - JOUR
AU  - Schaefer, Jorrit
AU  - Jovanović, Goran
AU  - Kotta-Loizou, Loly
AU  - Buck, Martin
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/935
AB  - Historically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate, o-nitrophenyl-ss-D-galactopyranoside (ONPG). However, the traditional method for measuring LacZ activity (first described by J. H. Miller in 1972) can be challenging for a large number of samples, is prone to variability, and involves hazardous compounds for lysis (e.g., chloroform, toluene). Here we describe a single-step assay using a 96-well microplate reader with a proven alternative cell permeabilization method. This modified protocol reduces handling time by 90%.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Analytical Biochemistry
T1  - Single-step method for beta-galactosidase assays in Escherichia coli using a 96-well microplate reader
EP  - 57
SP  - 56
VL  - 503
DO  - 10.1016/j.ab.2016.03.017
ER  - 

@article{
author = "Schaefer, Jorrit and Jovanović, Goran and Kotta-Loizou, Loly and Buck, Martin",
year = "2016",
abstract = "Historically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate, o-nitrophenyl-ss-D-galactopyranoside (ONPG). However, the traditional method for measuring LacZ activity (first described by J. H. Miller in 1972) can be challenging for a large number of samples, is prone to variability, and involves hazardous compounds for lysis (e.g., chloroform, toluene). Here we describe a single-step assay using a 96-well microplate reader with a proven alternative cell permeabilization method. This modified protocol reduces handling time by 90%.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "Single-step method for beta-galactosidase assays in Escherichia coli using a 96-well microplate reader",
pages = "57-56",
volume = "503",
doi = "10.1016/j.ab.2016.03.017"
}

Schaefer, J., Jovanović, G., Kotta-Loizou, L.,& Buck, M.. (2016). Single-step method for beta-galactosidase assays in Escherichia coli using a 96-well microplate reader. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 503, 56-57.
https://doi.org/10.1016/j.ab.2016.03.017

Schaefer J, Jovanović G, Kotta-Loizou L, Buck M. Single-step method for beta-galactosidase assays in Escherichia coli using a 96-well microplate reader. in Analytical Biochemistry. 2016;503:56-57.
doi:10.1016/j.ab.2016.03.017 .

Schaefer, Jorrit, Jovanović, Goran, Kotta-Loizou, Loly, Buck, Martin, "Single-step method for beta-galactosidase assays in Escherichia coli using a 96-well microplate reader" in Analytical Biochemistry, 503 (2016):56-57,
https://doi.org/10.1016/j.ab.2016.03.017 . .