TY  - JOUR
AU  - Bratić, Ana M.
AU  - Majić, Dragana 
AU  - Samardžić, Jelena
AU  - Kragl, M.W.
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/428
AB  - Ekspresija gena je regulisana posredstvom promotora-DNK sekvenci uzvodno od kodirajućeg regiona. Promotori svojom sekvencom određuju mesta vezivanja transkripcionih faktora, regulatora i RNK polimeraze. Strukturna i funkcionalna analiza promotora neophodna je za razumevanje mehanizama genske ekspresije. Cilj ovog rada je razvijanje brzog, efikasnog i pouzdanog sistema za testiranje bazalne promotorske aktivnosti kao i otkrivanje sekvenci polen-specifičnih elemenata promotora. U ovom radu je testirana funkcionalnost promotora za metalotionein heljde, posredstvom histohemijskog GUS-eseja u dva tranzijentna sistema za ekspresiju: BY2 ćelijama i polenovim zrnima. U oba sistema je uočena izražena aktivnost ispitivanog promotora.
AB  - Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena
T1  - Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes
EP  - 62
IS  - 1
SP  - 57
VL  - 62
DO  - 10.2298/ABS1001057B
ER  - 

@article{
author = "Bratić, Ana M. and Majić, Dragana  and Samardžić, Jelena and Kragl, M.W. and Maksimović, Vesna R.",
year = "2010",
abstract = "Ekspresija gena je regulisana posredstvom promotora-DNK sekvenci uzvodno od kodirajućeg regiona. Promotori svojom sekvencom određuju mesta vezivanja transkripcionih faktora, regulatora i RNK polimeraze. Strukturna i funkcionalna analiza promotora neophodna je za razumevanje mehanizama genske ekspresije. Cilj ovog rada je razvijanje brzog, efikasnog i pouzdanog sistema za testiranje bazalne promotorske aktivnosti kao i otkrivanje sekvenci polen-specifičnih elemenata promotora. U ovom radu je testirana funkcionalnost promotora za metalotionein heljde, posredstvom histohemijskog GUS-eseja u dva tranzijentna sistema za ekspresiju: BY2 ćelijama i polenovim zrnima. U oba sistema je uočena izražena aktivnost ispitivanog promotora., Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena, Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes",
pages = "62-57",
number = "1",
volume = "62",
doi = "10.2298/ABS1001057B"
}

Bratić, A. M., Majić, D., Samardžić, J., Kragl, M.W.,& Maksimović, V. R.. (2010). Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(1), 57-62.
https://doi.org/10.2298/ABS1001057B

Bratić AM, Majić D, Samardžić J, Kragl M, Maksimović VR. Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena. in Archives of Biological Sciences. 2010;62(1):57-62.
doi:10.2298/ABS1001057B .

Bratić, Ana M., Majić, Dragana , Samardžić, Jelena, Kragl, M.W., Maksimović, Vesna R., "Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena" in Archives of Biological Sciences, 62, no. 1 (2010):57-62,
https://doi.org/10.2298/ABS1001057B . .

TY  - JOUR
AU  - Nikolić, Dragana
AU  - Samardžić, Jelena
AU  - Bratić, Ana M.
AU  - Radin, Ivan P.
AU  - Gavrilović, Srdjan P.
AU  - Rausch, Thomas
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/417
AB  - The protective role in vivo of buckwheat metallothionein type 3 (FeMT3) during metal stress and the responsiveness of its promoter to metal ions were examined. Increased tolerance to heavy metals of FeMT3 producing Escherichia coli and cup1(Delta) yeast cells was detected. The defensive ability of buckwheat MT3 during Cd and Cu stresses was also demonstrated in Nicotiana debneyii leaves transiently expressing FeMT3. In contrast to phytochelatins, the cytoplasmatic localization of FeMT3 was not altered under heavy metal stress. Functional analysis of the corresponding promoter region revealed extremely high inducibility upon Cu2+ and Cd2+ treatments. The confirmed defense ability of FeMT3 protein in vivo and the great responsiveness of its promoter during heavy metal exposure make this gene a suitable candidate for biotechnological applications.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments
EP  - 3494
IS  - 6
SP  - 3488
VL  - 58
DO  - 10.1021/jf904483a
ER  - 

@article{
author = "Nikolić, Dragana and Samardžić, Jelena and Bratić, Ana M. and Radin, Ivan P. and Gavrilović, Srdjan P. and Rausch, Thomas and Maksimović, Vesna R.",
year = "2010",
abstract = "The protective role in vivo of buckwheat metallothionein type 3 (FeMT3) during metal stress and the responsiveness of its promoter to metal ions were examined. Increased tolerance to heavy metals of FeMT3 producing Escherichia coli and cup1(Delta) yeast cells was detected. The defensive ability of buckwheat MT3 during Cd and Cu stresses was also demonstrated in Nicotiana debneyii leaves transiently expressing FeMT3. In contrast to phytochelatins, the cytoplasmatic localization of FeMT3 was not altered under heavy metal stress. Functional analysis of the corresponding promoter region revealed extremely high inducibility upon Cu2+ and Cd2+ treatments. The confirmed defense ability of FeMT3 protein in vivo and the great responsiveness of its promoter during heavy metal exposure make this gene a suitable candidate for biotechnological applications.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments",
pages = "3494-3488",
number = "6",
volume = "58",
doi = "10.1021/jf904483a"
}

Nikolić, D., Samardžić, J., Bratić, A. M., Radin, I. P., Gavrilović, S. P., Rausch, T.,& Maksimović, V. R.. (2010). Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 58(6), 3488-3494.
https://doi.org/10.1021/jf904483a

Nikolić D, Samardžić J, Bratić AM, Radin IP, Gavrilović SP, Rausch T, Maksimović VR. Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments. in Journal of Agricultural and Food Chemistry. 2010;58(6):3488-3494.
doi:10.1021/jf904483a .

Nikolić, Dragana, Samardžić, Jelena, Bratić, Ana M., Radin, Ivan P., Gavrilović, Srdjan P., Rausch, Thomas, Maksimović, Vesna R., "Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments" in Journal of Agricultural and Food Chemistry, 58, no. 6 (2010):3488-3494,
https://doi.org/10.1021/jf904483a . .

TY  - JOUR
AU  - Bratić, Ana M.
AU  - Nikolić, Dragana
AU  - Samardžić, Jelena
AU  - Maksimović, Vesna R.
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/355
AB  - To shed light on expression regulation of the metallothionein gene from buckwheat (FeMT3), functional promoter analysis was performed with a complete 5' regulatory region and two deletion variants, employing stably transformed tobacco plants. Histochemical GUS assay of transgenic tobacco lines showed the strongest. signals in vascular elements of leaves and in pollen grains, while somewhat weaker staining was observed in the roots of mature plants. This tissue specificity pattern implies a possible function of buckwheat MT3 in those tissues. Quantitative GUS assay showed strong up-regulation of all three promoter constructs (proportional to the length of the regulatory region) in leaves submerged in liquid MS medium containing sucrose, after a prolonged time period. This represented a complex stress situation composed of several synergistically related stress stimuli. These findings suggest: complex transcriptional regulation of FeMT3, requiring interactions among a number of different factors.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli
EP  - 1000
IS  - 9
SP  - 996
VL  - 166
DO  - 10.1016/j.jplph.2008.12.002
ER  - 

@article{
author = "Bratić, Ana M. and Nikolić, Dragana and Samardžić, Jelena and Maksimović, Vesna R.",
year = "2009",
abstract = "To shed light on expression regulation of the metallothionein gene from buckwheat (FeMT3), functional promoter analysis was performed with a complete 5' regulatory region and two deletion variants, employing stably transformed tobacco plants. Histochemical GUS assay of transgenic tobacco lines showed the strongest. signals in vascular elements of leaves and in pollen grains, while somewhat weaker staining was observed in the roots of mature plants. This tissue specificity pattern implies a possible function of buckwheat MT3 in those tissues. Quantitative GUS assay showed strong up-regulation of all three promoter constructs (proportional to the length of the regulatory region) in leaves submerged in liquid MS medium containing sucrose, after a prolonged time period. This represented a complex stress situation composed of several synergistically related stress stimuli. These findings suggest: complex transcriptional regulation of FeMT3, requiring interactions among a number of different factors.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli",
pages = "1000-996",
number = "9",
volume = "166",
doi = "10.1016/j.jplph.2008.12.002"
}

Bratić, A. M., Nikolić, D., Samardžić, J.,& Maksimović, V. R.. (2009). Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli. in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 166(9), 996-1000.
https://doi.org/10.1016/j.jplph.2008.12.002

Bratić AM, Nikolić D, Samardžić J, Maksimović VR. Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli. in Journal of Plant Physiology. 2009;166(9):996-1000.
doi:10.1016/j.jplph.2008.12.002 .

Bratić, Ana M., Nikolić, Dragana, Samardžić, Jelena, Maksimović, Vesna R., "Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli" in Journal of Plant Physiology, 166, no. 9 (2009):996-1000,
https://doi.org/10.1016/j.jplph.2008.12.002 . .