TY  - JOUR
AU  - Lazarević, Milica
AU  - Jovanović, Nikola
AU  - Cvetković, Vladimir J.
AU  - Tošić, Svetlana
AU  - Vitorović, Jelena
AU  - Stamenković, Slaviša
AU  - Nikolov, Vesna
AU  - Vidović, Nataša
AU  - Kostić Perić, Jelena
AU  - Jovanović, Marija
AU  - Mitrović, Tatjana
PY  - 2023
UR  - https://www.mdpi.com/2075-4418/13/3/360
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1777
AB  - Comparative analysis of the conventional methylation-specific PCR (MSP) vs. the quantitative MSP (qMSP) assessment of the O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status in 34 snap-frozen (SF) glioma samples was performed. The accuracy of the semi-quantitative MSP was compared with the corresponding qMSP semi-quantitative values using two semi-quantitative cut-off values (0—unmethylated and 1—weakly methylated) to discriminate methylated from unmethylated samples. In the case of the cut-off value 0, MSP test showed 80.0% sensitivity and 78.9% specificity compared to the reference qMSP analysis. However, when using the cut-off value 1, the diagnostic accuracy of the MSP test was significantly higher (85.7% sensitivity, 85.2% specificity). Fleiss’ Kappa statistical analyses indicated moderate agreement (Fleiss’ Kappa Coefficient = 0.509; 70.59% agreement) between MSP and qMSP semi-quantitative measurements of MGMT promoter methylation in glioma patients, justifying the conventional MSP use in diagnostics and confirming its high reliability. Further, we aimed to compare the validity of SF and formalin-fixed paraffin-embedded (FFPE) glioma samples for MGMT testing. Statistical analyses indicated moderate overall agreement of FFPE glioma samples and SF MSP semi-quantitative measurements (Fleiss’ Kappa Coefficient = 0.516/0.509; 70.0% agreement) and emphasized their low reliability in the assessment of highly methylated MGMT promoter samples.
T2  - Diagnostics
T2  - Diagnostics
T1  - A Comparison of MGMT Testing by MSP and qMSP in Paired Snap-Frozen and Formalin-Fixed Paraffin-Embedded Gliomas
IS  - 3
SP  - 360
VL  - 13
DO  - 10.3390/diagnostics13030360
ER  - 

@article{
author = "Lazarević, Milica and Jovanović, Nikola and Cvetković, Vladimir J. and Tošić, Svetlana and Vitorović, Jelena and Stamenković, Slaviša and Nikolov, Vesna and Vidović, Nataša and Kostić Perić, Jelena and Jovanović, Marija and Mitrović, Tatjana",
year = "2023",
abstract = "Comparative analysis of the conventional methylation-specific PCR (MSP) vs. the quantitative MSP (qMSP) assessment of the O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status in 34 snap-frozen (SF) glioma samples was performed. The accuracy of the semi-quantitative MSP was compared with the corresponding qMSP semi-quantitative values using two semi-quantitative cut-off values (0—unmethylated and 1—weakly methylated) to discriminate methylated from unmethylated samples. In the case of the cut-off value 0, MSP test showed 80.0% sensitivity and 78.9% specificity compared to the reference qMSP analysis. However, when using the cut-off value 1, the diagnostic accuracy of the MSP test was significantly higher (85.7% sensitivity, 85.2% specificity). Fleiss’ Kappa statistical analyses indicated moderate agreement (Fleiss’ Kappa Coefficient = 0.509; 70.59% agreement) between MSP and qMSP semi-quantitative measurements of MGMT promoter methylation in glioma patients, justifying the conventional MSP use in diagnostics and confirming its high reliability. Further, we aimed to compare the validity of SF and formalin-fixed paraffin-embedded (FFPE) glioma samples for MGMT testing. Statistical analyses indicated moderate overall agreement of FFPE glioma samples and SF MSP semi-quantitative measurements (Fleiss’ Kappa Coefficient = 0.516/0.509; 70.0% agreement) and emphasized their low reliability in the assessment of highly methylated MGMT promoter samples.",
journal = "Diagnostics, Diagnostics",
title = "A Comparison of MGMT Testing by MSP and qMSP in Paired Snap-Frozen and Formalin-Fixed Paraffin-Embedded Gliomas",
number = "3",
pages = "360",
volume = "13",
doi = "10.3390/diagnostics13030360"
}

Lazarević, M., Jovanović, N., Cvetković, V. J., Tošić, S., Vitorović, J., Stamenković, S., Nikolov, V., Vidović, N., Kostić Perić, J., Jovanović, M.,& Mitrović, T.. (2023). A Comparison of MGMT Testing by MSP and qMSP in Paired Snap-Frozen and Formalin-Fixed Paraffin-Embedded Gliomas. in Diagnostics, 13(3), 360.
https://doi.org/10.3390/diagnostics13030360

Lazarević M, Jovanović N, Cvetković VJ, Tošić S, Vitorović J, Stamenković S, Nikolov V, Vidović N, Kostić Perić J, Jovanović M, Mitrović T. A Comparison of MGMT Testing by MSP and qMSP in Paired Snap-Frozen and Formalin-Fixed Paraffin-Embedded Gliomas. in Diagnostics. 2023;13(3):360.
doi:10.3390/diagnostics13030360 .

Lazarević, Milica, Jovanović, Nikola, Cvetković, Vladimir J., Tošić, Svetlana, Vitorović, Jelena, Stamenković, Slaviša, Nikolov, Vesna, Vidović, Nataša, Kostić Perić, Jelena, Jovanović, Marija, Mitrović, Tatjana, "A Comparison of MGMT Testing by MSP and qMSP in Paired Snap-Frozen and Formalin-Fixed Paraffin-Embedded Gliomas" in Diagnostics, 13, no. 3 (2023):360,
https://doi.org/10.3390/diagnostics13030360 . .