TY  - CONF
AU  - Ponjavić, Marijana
AU  - Jeremić, Sanja
AU  - Malagurski, Ivana
AU  - Babu P., Ramesh
AU  - Rajasekaran, Divya
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2236
AB  - The rapid increase in global plastics production is
also causing an accelerated environmental
pollution. Recently, biotechnological solutions and
enzymatic recycling of poly(ethylene terephthalate)
(PET) waste stream have been put forward and
commercialized1. Increasing recycling and
upcycling rates is the most effective model
approach to plastic circularity. However, mixed
plastic waste is still quite a challenge for both
recycling and upcycling technologies. This study is
focused on the eco-conversion of plastic waste
containing poly(ethylene terephthalate), PET, into
biopolymer, bacterial nanocellulose. Polymer mix
contained selection of commercial biodegradable
plastics (poly(lactic acid), PLA, poly(ε-caprolactone),
PCL, poly(hyoxyl butyrate), PHB) and PET. This
mixture was hydrolysed under aqueous conditions
and hydrolysate was used as carbon source for
Komagataeibacter medellinensis ID13488 and
bacterial nanocellulose (BNC) production. HPLC
analysis confirmed the presence of monomers and
dimers of polymer mix components indicating
existence of potential substrates for BNC
production. BNC production by K. medellinensis
was investigated and optimized in ter of the
amount of carbon source and growth conditions.
Under the most efficient rate in ter of yield, BNC
production was scaled up and the obtained
biopolymer was characterized. The structure of
produced BNC was confirmed by FTIR analysis,
thermal properties by DSC/TG analysis, and the
morphology of material by optical microscopy and
SEM analysis. This research demonstrates how to
put the mixed plastic waste stream into a circular
loop through the biotechnological conversion into
valuable biopolymer.
C3  - 10th Conference of Mikrobiokosmos
T1  - Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2236
ER  - 

@conference{
author = "Ponjavić, Marijana and Jeremić, Sanja and Malagurski, Ivana and Babu P., Ramesh and Rajasekaran, Divya and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "The rapid increase in global plastics production is
also causing an accelerated environmental
pollution. Recently, biotechnological solutions and
enzymatic recycling of poly(ethylene terephthalate)
(PET) waste stream have been put forward and
commercialized1. Increasing recycling and
upcycling rates is the most effective model
approach to plastic circularity. However, mixed
plastic waste is still quite a challenge for both
recycling and upcycling technologies. This study is
focused on the eco-conversion of plastic waste
containing poly(ethylene terephthalate), PET, into
biopolymer, bacterial nanocellulose. Polymer mix
contained selection of commercial biodegradable
plastics (poly(lactic acid), PLA, poly(ε-caprolactone),
PCL, poly(hyoxyl butyrate), PHB) and PET. This
mixture was hydrolysed under aqueous conditions
and hydrolysate was used as carbon source for
Komagataeibacter medellinensis ID13488 and
bacterial nanocellulose (BNC) production. HPLC
analysis confirmed the presence of monomers and
dimers of polymer mix components indicating
existence of potential substrates for BNC
production. BNC production by K. medellinensis
was investigated and optimized in ter of the
amount of carbon source and growth conditions.
Under the most efficient rate in ter of yield, BNC
production was scaled up and the obtained
biopolymer was characterized. The structure of
produced BNC was confirmed by FTIR analysis,
thermal properties by DSC/TG analysis, and the
morphology of material by optical microscopy and
SEM analysis. This research demonstrates how to
put the mixed plastic waste stream into a circular
loop through the biotechnological conversion into
valuable biopolymer.",
journal = "10th Conference of Mikrobiokosmos",
title = "Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2236"
}

Ponjavić, M., Jeremić, S., Malagurski, I., Babu P., R., Rajasekaran, D., Topakas, E.,& Nikodinović-Runić, J.. (2023). Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose. in 10th Conference of Mikrobiokosmos.
https://hdl.handle.net/21.15107/rcub_imagine_2236

Ponjavić M, Jeremić S, Malagurski I, Babu P. R, Rajasekaran D, Topakas E, Nikodinović-Runić J. Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose. in 10th Conference of Mikrobiokosmos. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2236 .

Ponjavić, Marijana, Jeremić, Sanja, Malagurski, Ivana, Babu P., Ramesh, Rajasekaran, Divya, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose" in 10th Conference of Mikrobiokosmos (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2236 .

TY  - CONF
AU  - Ponjavić, Marijana
AU  - Babu P., Ramesh
AU  - Rajasekaran, Divya
AU  - Pantelić, Brana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.accelevents.com/e/circular-bioeconomy-2023#about
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1924
AB  - Large amounts of polymers are discarded worldwide each year, leading to a significant
polymer waste in natural environment. The upcycling has been found as an efficient way to
transform polymer waste into high-value biomaterials meeting the conditions required for
circularity by being indefinitely recyclable, without reduction in value or usability.
The presented study refers to the upcycling of commercial biopolymers into bacterial
nanocellulose. Polymer blends, consisted of biodegradable polymers, such as poly(lactic acid),
PLA, poly(butylene succinate), PBS, and poly(ε-caprolactone), PCL. Polymers were hydrolyzed
and the obtained hydrolysates were investigated as potential carbon source for
K. medellinensis ID13488 growth and nanocellulose production. Degradation products were
analyzed using HPLC analysis. Different growth media, including tap water, HS medium,
absence / presence of glucose, were tested and bacterial nanocellulose growth was
confirmed under the most of the tested conditions. Once the BNC growth was set up, the BNC
production was scaled up and the obtained material was investigated in terms of structure
confirmation (FTIR analysis), thermal properties (DSC/TG analysis), morphology (optical
microscopy, AFM analysis) and crystallinity (XRD analysis). Finally, the full life cycle of mixed
biopolymers: from biodegradation to revalorization of end products into bacterial
nanocellulose appeared as perfect model approach to plastic circularity.
C3  - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming
T1  - Revalorization of biodegradable polymers to valuable bacterial nanocellulose
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1924
ER  - 

@conference{
author = "Ponjavić, Marijana and Babu P., Ramesh and Rajasekaran, Divya and Pantelić, Brana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Large amounts of polymers are discarded worldwide each year, leading to a significant
polymer waste in natural environment. The upcycling has been found as an efficient way to
transform polymer waste into high-value biomaterials meeting the conditions required for
circularity by being indefinitely recyclable, without reduction in value or usability.
The presented study refers to the upcycling of commercial biopolymers into bacterial
nanocellulose. Polymer blends, consisted of biodegradable polymers, such as poly(lactic acid),
PLA, poly(butylene succinate), PBS, and poly(ε-caprolactone), PCL. Polymers were hydrolyzed
and the obtained hydrolysates were investigated as potential carbon source for
K. medellinensis ID13488 growth and nanocellulose production. Degradation products were
analyzed using HPLC analysis. Different growth media, including tap water, HS medium,
absence / presence of glucose, were tested and bacterial nanocellulose growth was
confirmed under the most of the tested conditions. Once the BNC growth was set up, the BNC
production was scaled up and the obtained material was investigated in terms of structure
confirmation (FTIR analysis), thermal properties (DSC/TG analysis), morphology (optical
microscopy, AFM analysis) and crystallinity (XRD analysis). Finally, the full life cycle of mixed
biopolymers: from biodegradation to revalorization of end products into bacterial
nanocellulose appeared as perfect model approach to plastic circularity.",
journal = "Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming",
title = "Revalorization of biodegradable polymers to valuable bacterial nanocellulose",
pages = "55",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1924"
}

Ponjavić, M., Babu P., R., Rajasekaran, D., Pantelić, B.,& Nikodinović-Runić, J.. (2023). Revalorization of biodegradable polymers to valuable bacterial nanocellulose. in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 55.
https://hdl.handle.net/21.15107/rcub_imagine_1924

Ponjavić M, Babu P. R, Rajasekaran D, Pantelić B, Nikodinović-Runić J. Revalorization of biodegradable polymers to valuable bacterial nanocellulose. in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming. 2023;:55.
https://hdl.handle.net/21.15107/rcub_imagine_1924 .

Ponjavić, Marijana, Babu P., Ramesh, Rajasekaran, Divya, Pantelić, Brana, Nikodinović-Runić, Jasmina, "Revalorization of biodegradable polymers to valuable bacterial nanocellulose" in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming (2023):55,
https://hdl.handle.net/21.15107/rcub_imagine_1924 .

TY  - JOUR
AU  - Sourkouni, Georgia
AU  - Jeremić, Sanja
AU  - Kalogirou, Charalampia
AU  - Höfft, Oliver
AU  - Nenadović, Marija
AU  - Janković, Vukašin
AU  - Rajasekaran, Divya
AU  - Pandis, Pavlos
AU  - Padamati, Ramesh
AU  - Nikodinović-Runić, Jasmina
AU  - Argirusis, Christos
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1804
AB  - It is well acknowledged that microplastics are a major environmental problem and that the use of plastics, both petro- and bio- based, should be reduced. Nevertheless, it is also a necessity to reduce the amount of the already spread plastics. These cannot be easily degraded in the nature and accumulate in the food supply chain with major danger for animals and human life. It has been shown in the literature that advanced oxidation processes (AOPs) modify the surface of polylactic acid (PLA) materials in a way that bacteria more efficiently dock on their surface and eventually degrade them. In the present work we investigated the influence of different AOPs (ultrasounds, ultraviolet irradiation, and their combination) on the biodegradability of PLA films treated for different times between 1 and 6 h. The pre-treated samples have been degraded using a home model compost as well as a cocktail of commercial enzymes at mesophilic temperatures (37 °C and 42 °C, respectively). Degradation degree has been measured and degradation products have been identified. Excellent degradation of PLA films has been achieved with enzyme cocktail containing commercial alkaline proteases and lipases of up to 90% weight loss. For the first time, we also report valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.
T2  - World Journal of Microbiology and Biotechnology
T2  - World Journal of Microbiology and BiotechnologyWorld J Microbiol Biotechnol
T1  - Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose
IS  - 6
SP  - 161
VL  - 39
DO  - 10.1007/s11274-023-03605-4
ER  - 

@article{
author = "Sourkouni, Georgia and Jeremić, Sanja and Kalogirou, Charalampia and Höfft, Oliver and Nenadović, Marija and Janković, Vukašin and Rajasekaran, Divya and Pandis, Pavlos and Padamati, Ramesh and Nikodinović-Runić, Jasmina and Argirusis, Christos",
year = "2023",
abstract = "It is well acknowledged that microplastics are a major environmental problem and that the use of plastics, both petro- and bio- based, should be reduced. Nevertheless, it is also a necessity to reduce the amount of the already spread plastics. These cannot be easily degraded in the nature and accumulate in the food supply chain with major danger for animals and human life. It has been shown in the literature that advanced oxidation processes (AOPs) modify the surface of polylactic acid (PLA) materials in a way that bacteria more efficiently dock on their surface and eventually degrade them. In the present work we investigated the influence of different AOPs (ultrasounds, ultraviolet irradiation, and their combination) on the biodegradability of PLA films treated for different times between 1 and 6 h. The pre-treated samples have been degraded using a home model compost as well as a cocktail of commercial enzymes at mesophilic temperatures (37 °C and 42 °C, respectively). Degradation degree has been measured and degradation products have been identified. Excellent degradation of PLA films has been achieved with enzyme cocktail containing commercial alkaline proteases and lipases of up to 90% weight loss. For the first time, we also report valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.",
journal = "World Journal of Microbiology and Biotechnology, World Journal of Microbiology and BiotechnologyWorld J Microbiol Biotechnol",
title = "Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose",
number = "6",
pages = "161",
volume = "39",
doi = "10.1007/s11274-023-03605-4"
}

Sourkouni, G., Jeremić, S., Kalogirou, C., Höfft, O., Nenadović, M., Janković, V., Rajasekaran, D., Pandis, P., Padamati, R., Nikodinović-Runić, J.,& Argirusis, C.. (2023). Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose. in World Journal of Microbiology and Biotechnology, 39(6), 161.
https://doi.org/10.1007/s11274-023-03605-4

Sourkouni G, Jeremić S, Kalogirou C, Höfft O, Nenadović M, Janković V, Rajasekaran D, Pandis P, Padamati R, Nikodinović-Runić J, Argirusis C. Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose. in World Journal of Microbiology and Biotechnology. 2023;39(6):161.
doi:10.1007/s11274-023-03605-4 .

Sourkouni, Georgia, Jeremić, Sanja, Kalogirou, Charalampia, Höfft, Oliver, Nenadović, Marija, Janković, Vukašin, Rajasekaran, Divya, Pandis, Pavlos, Padamati, Ramesh, Nikodinović-Runić, Jasmina, Argirusis, Christos, "Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose" in World Journal of Microbiology and Biotechnology, 39, no. 6 (2023):161,
https://doi.org/10.1007/s11274-023-03605-4 . .