TY  - CONF
AU  - Trivanović, Drenka
AU  - Kovačević, Jovana
AU  - Arsić, Aleksandra
AU  - Vujačić, Marko
AU  - Bogosavljević, Nikola
AU  - Okić Djordjević, Ivana
AU  - Živanović, Milena
AU  - Mojsilović, Slavko
AU  - Maljković, Mirjana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1995
AB  - As adipose tissue (AT) undergoes metabolic reprogramming with age, we investigated
skeletal site-specific and age-dependent lipid profile of bone marrow adipose tissue (BMAT).
Acetabular and femoral BMAT, and gluteofemoral subcutaneous adipose tissue (gfSAT)
were obtained from matched osteoarthritis patients. Patients were classified into two
groups: younger (≤ 60 years) and aged (>60 years) adults. BMAT and gfSAT were explored
by using thin layer/gas chromatography coupled with cellular and molecular assays.
Data were interpreted and visualized by applying linear discriminant analysis (LDA) and
hierarchical clustering of fatty acid (FA) composition. Statistics was estimated by nonparametric
tests and Spearman’s rank correlation.
Analyses of total lipids revealed significantly reduced triglyceride content in femoral
(fBMAT) than in acetabular BMAT (aBMAT) and gfSAT. Frequencies of spontaneously
released saturated palmitic (C16:0) and stearic acids (C18:0) were higher in fBMAT than
in aBMAT and gfSAT (p=0.036 and p=0.046, n=8). Cluster heatmap and LDA showed that
fBMAT differed to acetabular and gfSAT, while acetabular and gfSAT were more similar in
FA profiles. FA profiles of AT depots varied with patient’s age. Contribution of palmitic acid
was increased in aged group in all AT depots, while stearic acid declined in aged group in
BMAT compartments only. fBMAT cellularity declined with age (r=-0.675, n=14, p=0.037).
Additionally, the presence of CD45-CD31-CD34+CD24+ adipogenic progenitor (stem) cells
was increased in fBMAT (0.46±0.03%) when compared to aBMAT (0.21±0.01%) depot.
Femoral mesenchymal stem cells displayed pronounced adipogenesis comparing to their
acetabular counterparts.
Our findings suggest that specific lipid profile of fBMAT imposes adipogenic commitment
of stem cells within this skeletal site.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity
EP  - 54
SP  - 54
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1995
ER  - 

@conference{
author = "Trivanović, Drenka and Kovačević, Jovana and Arsić, Aleksandra and Vujačić, Marko and Bogosavljević, Nikola and Okić Djordjević, Ivana and Živanović, Milena and Mojsilović, Slavko and Maljković, Mirjana and Jauković, Aleksandra",
year = "2023",
abstract = "As adipose tissue (AT) undergoes metabolic reprogramming with age, we investigated
skeletal site-specific and age-dependent lipid profile of bone marrow adipose tissue (BMAT).
Acetabular and femoral BMAT, and gluteofemoral subcutaneous adipose tissue (gfSAT)
were obtained from matched osteoarthritis patients. Patients were classified into two
groups: younger (≤ 60 years) and aged (>60 years) adults. BMAT and gfSAT were explored
by using thin layer/gas chromatography coupled with cellular and molecular assays.
Data were interpreted and visualized by applying linear discriminant analysis (LDA) and
hierarchical clustering of fatty acid (FA) composition. Statistics was estimated by nonparametric
tests and Spearman’s rank correlation.
Analyses of total lipids revealed significantly reduced triglyceride content in femoral
(fBMAT) than in acetabular BMAT (aBMAT) and gfSAT. Frequencies of spontaneously
released saturated palmitic (C16:0) and stearic acids (C18:0) were higher in fBMAT than
in aBMAT and gfSAT (p=0.036 and p=0.046, n=8). Cluster heatmap and LDA showed that
fBMAT differed to acetabular and gfSAT, while acetabular and gfSAT were more similar in
FA profiles. FA profiles of AT depots varied with patient’s age. Contribution of palmitic acid
was increased in aged group in all AT depots, while stearic acid declined in aged group in
BMAT compartments only. fBMAT cellularity declined with age (r=-0.675, n=14, p=0.037).
Additionally, the presence of CD45-CD31-CD34+CD24+ adipogenic progenitor (stem) cells
was increased in fBMAT (0.46±0.03%) when compared to aBMAT (0.21±0.01%) depot.
Femoral mesenchymal stem cells displayed pronounced adipogenesis comparing to their
acetabular counterparts.
Our findings suggest that specific lipid profile of fBMAT imposes adipogenic commitment
of stem cells within this skeletal site.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity",
pages = "54-54",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1995"
}

Trivanović, D., Kovačević, J., Arsić, A., Vujačić, M., Bogosavljević, N., Okić Djordjević, I., Živanović, M., Mojsilović, S., Maljković, M.,& Jauković, A.. (2023). Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 54-54.
https://hdl.handle.net/21.15107/rcub_imagine_1995

Trivanović D, Kovačević J, Arsić A, Vujačić M, Bogosavljević N, Okić Djordjević I, Živanović M, Mojsilović S, Maljković M, Jauković A. Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity. in 4th Belgrade Bioinformatics Conference. 2023;4:54-54.
https://hdl.handle.net/21.15107/rcub_imagine_1995 .

Trivanović, Drenka, Kovačević, Jovana, Arsić, Aleksandra, Vujačić, Marko, Bogosavljević, Nikola, Okić Djordjević, Ivana, Živanović, Milena, Mojsilović, Slavko, Maljković, Mirjana, Jauković, Aleksandra, "Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity" in 4th Belgrade Bioinformatics Conference, 4 (2023):54-54,
https://hdl.handle.net/21.15107/rcub_imagine_1995 .

TY  - JOUR
AU  - Rajković, Katarina
AU  - Drobac, Milica
AU  - Milić, Petar
AU  - Vučić, Vesna
AU  - Arsić, Aleksandra
AU  - Perić, Mirjana
AU  - Radunović, Milena
AU  - Jeremić, Sanja
AU  - Arsenijević, Jelena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1805
AB  - Juglans nigra (Black walnut) is a source of health-promoting
biologically active compounds used in traditional medicine. The investigation of
bioactive compounds in black walnut could lead to its broader application, as
well as application of its by-products. Therefore, this study aimed to characterize
J. nigra nut and green husk based on chemical analysis of their petroleum ether
and ethanol extracts obtained by ultrasonic and reflux extraction methods,
respectively. Different extract fractions were tested for their antimicrobial
activities using Gram-negative bacteria (Escherichia coli, Pseudomonas
aeruginosa), Gram-positive bacteria (Enterococcus faecalis Staphylococcus
aureus) and yeast (reference strain and clinical isolates of Candida albicans).
Ethanol extracts analysis, performed by High Performance Liquid
Chromatography (HPLC), singled out the ellagic acid as the most dominant
compound in nut (55.0 ± 1.3 × 10-3 kg m-3
) and green husk (114.1 ± 0.5× 10-3 kg
m-3
) extracts. Non-polar compounds were evaluated using Gas Chromatography
(GC) analysis of petroleum ether extracts. Juglans nigra nut and green husk
contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid
(C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic
(C18:1n-9), and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids,
linoleic (C18:2n-6), γ-linolenic (C18:3n-6), and α-linolenic (C18:3n-3) acids.
Ethanol extracts of both J. nigra nut and green husk showed antimicrobial
activity against C. albicans which is the most common cause of yeast infections.
AB  - Juglans nigra (црни орах) као извор биолошки активних једињења користе се у
традиционалној медицини. Истраживање биоактивних једињења присутних у црном ораху
довело би до шире примене његових производа. Зато ова студија има за циљ да
окарактерише орашаст плод и зелену љуску Ј. nigra на основу хемијске анализе њихових
петролетарских и етанолних екстраката, добијених ултразвучним и рефлукс методама
екстракције. Антимикробна активност различитих фракција екстракта је тестирана
коришћењем Грам-негативних бактерија (Escherichia coli, Pseudomonas aeruginosa), Грамозитивних бактерија (Enterococcus faecalis Staphylococcus aureus) и квасница (референтни
сој и клинички изолати Candida albicans). Анализа течном хроматографијом високих
перформанси, издвојила је елагинску киселину као најдоминантније једињење у етанолним
екстрактима орашастог плода (55,0 ± 1,3 × 10-3 kg m-3) и зелене љуске (114,1 ± 0,5 × 10-3
g m-3). Неполарна једињења су процењена применом гасне хроматографске анализе
петролетарских екстракта. Орашаст плод и зелена љуска садрже две засићене масне
киселине, палмитинску (C16:0) и стеаринску киселину (C18:0), затим мононезасићене
масне киселине, палмитолеинску (C16:1n-7), олеинску (C18:1n-9), и вакценску киселину
(C18:1n-7), као и полинезасићене масне киселине, линолну (C18:2n-6), γ-линоленску
(C18:3n-6) и α-линоленску (C18: 3n-3) киселину. Етанолни екстракти орашастог плода и
зелене љуске показали су антимикробну активност нa сојевима C. albicans која је најчешћи
узрочник гљивичних инфекција.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk
T1  - Hemijska karakterizacija i antimikrobna aktivnost orašastog ploda i zelene ljuske Juglans nigra l.
T1  - Xемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra l.
DO  - doi.org/10.2298/JSC230210024R
ER  - 

@article{
author = "Rajković, Katarina and Drobac, Milica and Milić, Petar and Vučić, Vesna and Arsić, Aleksandra and Perić, Mirjana and Radunović, Milena and Jeremić, Sanja and Arsenijević, Jelena",
year = "2023",
abstract = "Juglans nigra (Black walnut) is a source of health-promoting
biologically active compounds used in traditional medicine. The investigation of
bioactive compounds in black walnut could lead to its broader application, as
well as application of its by-products. Therefore, this study aimed to characterize
J. nigra nut and green husk based on chemical analysis of their petroleum ether
and ethanol extracts obtained by ultrasonic and reflux extraction methods,
respectively. Different extract fractions were tested for their antimicrobial
activities using Gram-negative bacteria (Escherichia coli, Pseudomonas
aeruginosa), Gram-positive bacteria (Enterococcus faecalis Staphylococcus
aureus) and yeast (reference strain and clinical isolates of Candida albicans).
Ethanol extracts analysis, performed by High Performance Liquid
Chromatography (HPLC), singled out the ellagic acid as the most dominant
compound in nut (55.0 ± 1.3 × 10-3 kg m-3
) and green husk (114.1 ± 0.5× 10-3 kg
m-3
) extracts. Non-polar compounds were evaluated using Gas Chromatography
(GC) analysis of petroleum ether extracts. Juglans nigra nut and green husk
contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid
(C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic
(C18:1n-9), and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids,
linoleic (C18:2n-6), γ-linolenic (C18:3n-6), and α-linolenic (C18:3n-3) acids.
Ethanol extracts of both J. nigra nut and green husk showed antimicrobial
activity against C. albicans which is the most common cause of yeast infections., Juglans nigra (црни орах) као извор биолошки активних једињења користе се у
традиционалној медицини. Истраживање биоактивних једињења присутних у црном ораху
довело би до шире примене његових производа. Зато ова студија има за циљ да
окарактерише орашаст плод и зелену љуску Ј. nigra на основу хемијске анализе њихових
петролетарских и етанолних екстраката, добијених ултразвучним и рефлукс методама
екстракције. Антимикробна активност различитих фракција екстракта је тестирана
коришћењем Грам-негативних бактерија (Escherichia coli, Pseudomonas aeruginosa), Грамозитивних бактерија (Enterococcus faecalis Staphylococcus aureus) и квасница (референтни
сој и клинички изолати Candida albicans). Анализа течном хроматографијом високих
перформанси, издвојила је елагинску киселину као најдоминантније једињење у етанолним
екстрактима орашастог плода (55,0 ± 1,3 × 10-3 kg m-3) и зелене љуске (114,1 ± 0,5 × 10-3
g m-3). Неполарна једињења су процењена применом гасне хроматографске анализе
петролетарских екстракта. Орашаст плод и зелена љуска садрже две засићене масне
киселине, палмитинску (C16:0) и стеаринску киселину (C18:0), затим мононезасићене
масне киселине, палмитолеинску (C16:1n-7), олеинску (C18:1n-9), и вакценску киселину
(C18:1n-7), као и полинезасићене масне киселине, линолну (C18:2n-6), γ-линоленску
(C18:3n-6) и α-линоленску (C18: 3n-3) киселину. Етанолни екстракти орашастог плода и
зелене љуске показали су антимикробну активност нa сојевима C. albicans која је најчешћи
узрочник гљивичних инфекција.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk, Hemijska karakterizacija i antimikrobna aktivnost orašastog ploda i zelene ljuske Juglans nigra l., Xемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra l.",
doi = "doi.org/10.2298/JSC230210024R"
}

Rajković, K., Drobac, M., Milić, P., Vučić, V., Arsić, A., Perić, M., Radunović, M., Jeremić, S.,& Arsenijević, J.. (2023). Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society..
https://doi.org/doi.org/10.2298/JSC230210024R

Rajković K, Drobac M, Milić P, Vučić V, Arsić A, Perić M, Radunović M, Jeremić S, Arsenijević J. Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society. 2023;.
doi:doi.org/10.2298/JSC230210024R .

Rajković, Katarina, Drobac, Milica, Milić, Petar, Vučić, Vesna, Arsić, Aleksandra, Perić, Mirjana, Radunović, Milena, Jeremić, Sanja, Arsenijević, Jelena, "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk" in Journal of the Serbian Chemical Society (2023),
https://doi.org/doi.org/10.2298/JSC230210024R . .

TY  - CHAP
AU  - Kojadinović, Milica
AU  - Arsić, Aleksandra
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1716
AB  - Nar (Punica granatum, L) je jedan od najstarijih jestivih plodova koji se koristi u prevenciji i lečenju od davnina.
Različiti delovi biljke, kao što su plod, seme, kora i lišće, sadrže bioaktivne sastojke za koje se navodi da poseduju
lekovita i terapeutska svojstva. Međumnogobrojnimbioaktivnimkomponentama u naru, polifenoli
predstavljaju najvažniju grupumolekula sa pozitivnimefektomna zdravlje. Hipoglikemijski, hipolipemijski,
hipotenzivni i anti-oksidativni efekat polifenola nara potvrđen je u brojnimstudijama. Ovaj pregledni rad ima
za cilj da predstavi najnovija istraživanja o strukturi i biološkoj aktivnosti komponenata nara i njihovompotencijalnom
korisnom efektu u terapiji bolesti povezanih sa metaboličkim sindromom.
AB  - Pomegranate (Punica granatum, L) is one of the oldest edible fruits used in prevention and treatment. Various
parts of the plant, such as fruit, seeds, bark, and leaves, contain bioactive ingredients that possess medicinal
and therapeutic properties. Among the many bioactive components in pomegranate, polyphenols
are the most important group of molecules with a positive effect on health. The hypoglycemic, hypolipidemic,
hypotensive, and anti-oxidative effects of pomegranate polyphenols have been confirmed in numerous
studies. The aim of this review is to present the latest research on the structure and activity of
bioactive components of pomegranate and their potential beneficial effect in the treatment of diseases related
to metabolic syndrome.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
T2  - Trendovi u molekularnoj Biologiji
T1  - Biološko delovanje polifenola nara na komponente metaboličkog sindroma: implikacije na oksidativni stres
T1  - Biological effect of pomegranate polyphenols on the components of metabolic syndrome: implications on oxidative stress
EP  - 165
IS  - 1
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1716
ER  - 

@inbook{
author = "Kojadinović, Milica and Arsić, Aleksandra",
year = "2021",
abstract = "Nar (Punica granatum, L) je jedan od najstarijih jestivih plodova koji se koristi u prevenciji i lečenju od davnina.
Različiti delovi biljke, kao što su plod, seme, kora i lišće, sadrže bioaktivne sastojke za koje se navodi da poseduju
lekovita i terapeutska svojstva. Međumnogobrojnimbioaktivnimkomponentama u naru, polifenoli
predstavljaju najvažniju grupumolekula sa pozitivnimefektomna zdravlje. Hipoglikemijski, hipolipemijski,
hipotenzivni i anti-oksidativni efekat polifenola nara potvrđen je u brojnimstudijama. Ovaj pregledni rad ima
za cilj da predstavi najnovija istraživanja o strukturi i biološkoj aktivnosti komponenata nara i njihovompotencijalnom
korisnom efektu u terapiji bolesti povezanih sa metaboličkim sindromom., Pomegranate (Punica granatum, L) is one of the oldest edible fruits used in prevention and treatment. Various
parts of the plant, such as fruit, seeds, bark, and leaves, contain bioactive ingredients that possess medicinal
and therapeutic properties. Among the many bioactive components in pomegranate, polyphenols
are the most important group of molecules with a positive effect on health. The hypoglycemic, hypolipidemic,
hypotensive, and anti-oxidative effects of pomegranate polyphenols have been confirmed in numerous
studies. The aim of this review is to present the latest research on the structure and activity of
bioactive components of pomegranate and their potential beneficial effect in the treatment of diseases related
to metabolic syndrome.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
booktitle = "Biološko delovanje polifenola nara na komponente metaboličkog sindroma: implikacije na oksidativni stres, Biological effect of pomegranate polyphenols on the components of metabolic syndrome: implications on oxidative stress",
pages = "165-152",
number = "1",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1716"
}

Kojadinović, M.,& Arsić, A.. (2021). Biološko delovanje polifenola nara na komponente metaboličkog sindroma: implikacije na oksidativni stres. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo.(1), 152-165.
https://hdl.handle.net/21.15107/rcub_imagine_1716

Kojadinović M, Arsić A. Biološko delovanje polifenola nara na komponente metaboličkog sindroma: implikacije na oksidativni stres. in Trendovi u molekularnoj Biologiji. 2021;(1):152-165.
https://hdl.handle.net/21.15107/rcub_imagine_1716 .

Kojadinović, Milica, Arsić, Aleksandra, "Biološko delovanje polifenola nara na komponente metaboličkog sindroma: implikacije na oksidativni stres" in Trendovi u molekularnoj Biologiji, no. 1 (2021):152-165,
https://hdl.handle.net/21.15107/rcub_imagine_1716 .