TY  - JOUR
AU  - Ušjak, Dušan
AU  - Ivković, Branka
AU  - Božić, Dragana D.
AU  - Bošković, Lidija
AU  - Milenković, Marina
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0882401018319132
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1770
AB  - Acinetobacter baumannii and Pseudomonas aeruginosa are frequent multiresistant nosocomial pathogens that cause wound and pulmonary infections in hospitalized patients. As being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors. Chalcones are chemical compounds with well-documented antimicrobial potential. The aim of this study was to examine effectiveness of four newly-synthesized chalcones against the multiresistant clinical strains of A. baumannii and P. aeruginosa. Antibacterial activity of chalcones was investigated with broth-microdilution test and time-dependent killing assay. Synergistic effects of tested compounds with antibiotics (meropenem, amikacin and ciprofloxacin) were determined by checkerboard assay. The effects of chalcones on expression of virulence factors in P. aeruginosa (pyocyanin production, swimming and swarming motility) and A. baumannii (twitching and surface-associated motility), along with their biofilm production, were also examined. The obtained results indicate substantial antimicrobial activity of the tested chalcones (MICs = 100–175 μg/mL) and several synergistic interactions with antibiotics, as well as notable reduction in expression of all investigated virulence factors. These promising results may constitute a good basis for further research.
T2  - Microbial Pathogenesis
T2  - Microbial PathogenesisMicrobial Pathogenesis
T1  - Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa
EP  - 196
SP  - 186
VL  - 131
DO  - 10.1016/j.micpath.2019.04.015
ER  - 

@article{
author = "Ušjak, Dušan and Ivković, Branka and Božić, Dragana D. and Bošković, Lidija and Milenković, Marina",
year = "2019",
abstract = "Acinetobacter baumannii and Pseudomonas aeruginosa are frequent multiresistant nosocomial pathogens that cause wound and pulmonary infections in hospitalized patients. As being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors. Chalcones are chemical compounds with well-documented antimicrobial potential. The aim of this study was to examine effectiveness of four newly-synthesized chalcones against the multiresistant clinical strains of A. baumannii and P. aeruginosa. Antibacterial activity of chalcones was investigated with broth-microdilution test and time-dependent killing assay. Synergistic effects of tested compounds with antibiotics (meropenem, amikacin and ciprofloxacin) were determined by checkerboard assay. The effects of chalcones on expression of virulence factors in P. aeruginosa (pyocyanin production, swimming and swarming motility) and A. baumannii (twitching and surface-associated motility), along with their biofilm production, were also examined. The obtained results indicate substantial antimicrobial activity of the tested chalcones (MICs = 100–175 μg/mL) and several synergistic interactions with antibiotics, as well as notable reduction in expression of all investigated virulence factors. These promising results may constitute a good basis for further research.",
journal = "Microbial Pathogenesis, Microbial PathogenesisMicrobial Pathogenesis",
title = "Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa",
pages = "196-186",
volume = "131",
doi = "10.1016/j.micpath.2019.04.015"
}

Ušjak, D., Ivković, B., Božić, D. D., Bošković, L.,& Milenković, M.. (2019). Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa. in Microbial Pathogenesis, 131, 186-196.
https://doi.org/10.1016/j.micpath.2019.04.015

Ušjak D, Ivković B, Božić DD, Bošković L, Milenković M. Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa. in Microbial Pathogenesis. 2019;131:186-196.
doi:10.1016/j.micpath.2019.04.015 .

Ušjak, Dušan, Ivković, Branka, Božić, Dragana D., Bošković, Lidija, Milenković, Marina, "Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa" in Microbial Pathogenesis, 131 (2019):186-196,
https://doi.org/10.1016/j.micpath.2019.04.015 . .