TY  - JOUR
AU  - Cirković, Andja
AU  - Stanisavljević, Dejana
AU  - Milin-Lazović, Jelena
AU  - Rajović, Nina
AU  - Pavlović, Vedrana
AU  - Milicević, Ognjen
AU  - Savić, Marko
AU  - Perić, Jelena
AU  - Aleksić, Nataša
AU  - Milić, Nikola
AU  - Stanisavljević, Tamara
AU  - Miković, Zeljko
AU  - Garović, Vesna
AU  - Milić, Nataša
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1430
AB  - Introduction: Preeclampsia (PE) is a pregnancy-associated, multi-organ, life-threatening disease that appears after the 20th week of gestation. The aim of this study was to perform a systematic review and meta-analysis to determine whether women with PE have disrupted miRNA expression compared to women who do not have PE.Methods: We conducted a systematic review and meta-analysis of studies that reported miRNAs expression levels in placenta or peripheral blood of pregnant women with vs. without PE. Studies published before October 29, 2021 were identified through PubMed, EMBASE and Web of Science. Two reviewers used predefined forms and protocols to evaluate independently the eligibility of studies based on titles and abstracts and to perform full-text screening, data abstraction and quality assessment. Standardized mean difference (SMD) was used as a measure of effect size.Results: 229 publications were included in the systematic review and 53 in the meta-analysis. The expression levels in placenta were significantly higher in women with PE compared to women without PE for miRNA-16 (SMD = 1.51,95%CI = 0.55-2.46), miRNA-20b (SMD = 0.89, 95%CI = 0.33-1.45), miRNA-23a (SMD = 2.02, 95%CI = 1.25-2.78), miRNA-29b (SMD = 1.37, 95%CI = 0.36-2.37), miRNA-155 (SMD = 2.99, 95%CI = 0.83-5.14) and miRNA-210 (SMD = 1.63, 95%CI = 0.69-2.58), and significantly lower for miRNA-376c (SMD = -4.86, 95%CI = -9.51 to -0.20). An increased level of miRNK-155 expression was found in peripheral blood of women with PE (SMD = 2.06, 95%CI = 0.35-3.76), while the expression level of miRNA-16 was significantly lower in peripheral blood of PE women (SMD = -0.47, 95%CI = -0.91 to -0.03). The functional roles of the presented miRNAs include control of trophoblast proliferation, migration, invasion, apoptosis, differentiation, cellular metabolism and angiogenesis.Conclusion: miRNAs play an important role in the pathophysiology of PE. The identification of differentially expressed miRNAs in maternal blood creates an opportunity to define an easily accessible biomarker of PE.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Preeclamptic Women Have Disrupted Placental microRNA Expression at the Time of Preeclampsia Diagnosis: Meta-Analysis
VL  - 9
DO  - 10.3389/fbioe.2021.782845
ER  - 

@article{
author = "Cirković, Andja and Stanisavljević, Dejana and Milin-Lazović, Jelena and Rajović, Nina and Pavlović, Vedrana and Milicević, Ognjen and Savić, Marko and Perić, Jelena and Aleksić, Nataša and Milić, Nikola and Stanisavljević, Tamara and Miković, Zeljko and Garović, Vesna and Milić, Nataša",
year = "2021",
abstract = "Introduction: Preeclampsia (PE) is a pregnancy-associated, multi-organ, life-threatening disease that appears after the 20th week of gestation. The aim of this study was to perform a systematic review and meta-analysis to determine whether women with PE have disrupted miRNA expression compared to women who do not have PE.Methods: We conducted a systematic review and meta-analysis of studies that reported miRNAs expression levels in placenta or peripheral blood of pregnant women with vs. without PE. Studies published before October 29, 2021 were identified through PubMed, EMBASE and Web of Science. Two reviewers used predefined forms and protocols to evaluate independently the eligibility of studies based on titles and abstracts and to perform full-text screening, data abstraction and quality assessment. Standardized mean difference (SMD) was used as a measure of effect size.Results: 229 publications were included in the systematic review and 53 in the meta-analysis. The expression levels in placenta were significantly higher in women with PE compared to women without PE for miRNA-16 (SMD = 1.51,95%CI = 0.55-2.46), miRNA-20b (SMD = 0.89, 95%CI = 0.33-1.45), miRNA-23a (SMD = 2.02, 95%CI = 1.25-2.78), miRNA-29b (SMD = 1.37, 95%CI = 0.36-2.37), miRNA-155 (SMD = 2.99, 95%CI = 0.83-5.14) and miRNA-210 (SMD = 1.63, 95%CI = 0.69-2.58), and significantly lower for miRNA-376c (SMD = -4.86, 95%CI = -9.51 to -0.20). An increased level of miRNK-155 expression was found in peripheral blood of women with PE (SMD = 2.06, 95%CI = 0.35-3.76), while the expression level of miRNA-16 was significantly lower in peripheral blood of PE women (SMD = -0.47, 95%CI = -0.91 to -0.03). The functional roles of the presented miRNAs include control of trophoblast proliferation, migration, invasion, apoptosis, differentiation, cellular metabolism and angiogenesis.Conclusion: miRNAs play an important role in the pathophysiology of PE. The identification of differentially expressed miRNAs in maternal blood creates an opportunity to define an easily accessible biomarker of PE.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Preeclamptic Women Have Disrupted Placental microRNA Expression at the Time of Preeclampsia Diagnosis: Meta-Analysis",
volume = "9",
doi = "10.3389/fbioe.2021.782845"
}

Cirković, A., Stanisavljević, D., Milin-Lazović, J., Rajović, N., Pavlović, V., Milicević, O., Savić, M., Perić, J., Aleksić, N., Milić, N., Stanisavljević, T., Miković, Z., Garović, V.,& Milić, N.. (2021). Preeclamptic Women Have Disrupted Placental microRNA Expression at the Time of Preeclampsia Diagnosis: Meta-Analysis. in Frontiers in Bioengineering and Biotechnology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fbioe.2021.782845

Cirković A, Stanisavljević D, Milin-Lazović J, Rajović N, Pavlović V, Milicević O, Savić M, Perić J, Aleksić N, Milić N, Stanisavljević T, Miković Z, Garović V, Milić N. Preeclamptic Women Have Disrupted Placental microRNA Expression at the Time of Preeclampsia Diagnosis: Meta-Analysis. in Frontiers in Bioengineering and Biotechnology. 2021;9.
doi:10.3389/fbioe.2021.782845 .

Cirković, Andja, Stanisavljević, Dejana, Milin-Lazović, Jelena, Rajović, Nina, Pavlović, Vedrana, Milicević, Ognjen, Savić, Marko, Perić, Jelena, Aleksić, Nataša, Milić, Nikola, Stanisavljević, Tamara, Miković, Zeljko, Garović, Vesna, Milić, Nataša, "Preeclamptic Women Have Disrupted Placental microRNA Expression at the Time of Preeclampsia Diagnosis: Meta-Analysis" in Frontiers in Bioengineering and Biotechnology, 9 (2021),
https://doi.org/10.3389/fbioe.2021.782845 . .