TY  - JOUR
AU  - Bratić, Ana M.
AU  - Majić, Dragana 
AU  - Samardžić, Jelena
AU  - Kragl, M.W.
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/428
AB  - Ekspresija gena je regulisana posredstvom promotora-DNK sekvenci uzvodno od kodirajućeg regiona. Promotori svojom sekvencom određuju mesta vezivanja transkripcionih faktora, regulatora i RNK polimeraze. Strukturna i funkcionalna analiza promotora neophodna je za razumevanje mehanizama genske ekspresije. Cilj ovog rada je razvijanje brzog, efikasnog i pouzdanog sistema za testiranje bazalne promotorske aktivnosti kao i otkrivanje sekvenci polen-specifičnih elemenata promotora. U ovom radu je testirana funkcionalnost promotora za metalotionein heljde, posredstvom histohemijskog GUS-eseja u dva tranzijentna sistema za ekspresiju: BY2 ćelijama i polenovim zrnima. U oba sistema je uočena izražena aktivnost ispitivanog promotora.
AB  - Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena
T1  - Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes
EP  - 62
IS  - 1
SP  - 57
VL  - 62
DO  - 10.2298/ABS1001057B
ER  - 

@article{
author = "Bratić, Ana M. and Majić, Dragana  and Samardžić, Jelena and Kragl, M.W. and Maksimović, Vesna R.",
year = "2010",
abstract = "Ekspresija gena je regulisana posredstvom promotora-DNK sekvenci uzvodno od kodirajućeg regiona. Promotori svojom sekvencom određuju mesta vezivanja transkripcionih faktora, regulatora i RNK polimeraze. Strukturna i funkcionalna analiza promotora neophodna je za razumevanje mehanizama genske ekspresije. Cilj ovog rada je razvijanje brzog, efikasnog i pouzdanog sistema za testiranje bazalne promotorske aktivnosti kao i otkrivanje sekvenci polen-specifičnih elemenata promotora. U ovom radu je testirana funkcionalnost promotora za metalotionein heljde, posredstvom histohemijskog GUS-eseja u dva tranzijentna sistema za ekspresiju: BY2 ćelijama i polenovim zrnima. U oba sistema je uočena izražena aktivnost ispitivanog promotora., Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena, Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes",
pages = "62-57",
number = "1",
volume = "62",
doi = "10.2298/ABS1001057B"
}

Bratić, A. M., Majić, D., Samardžić, J., Kragl, M.W.,& Maksimović, V. R.. (2010). Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(1), 57-62.
https://doi.org/10.2298/ABS1001057B

Bratić AM, Majić D, Samardžić J, Kragl M, Maksimović VR. Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena. in Archives of Biological Sciences. 2010;62(1):57-62.
doi:10.2298/ABS1001057B .

Bratić, Ana M., Majić, Dragana , Samardžić, Jelena, Kragl, M.W., Maksimović, Vesna R., "Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena" in Archives of Biological Sciences, 62, no. 1 (2010):57-62,
https://doi.org/10.2298/ABS1001057B . .