TY  - JOUR
AU  - Timotijević, Gordana
AU  - Petković, Filip
AU  - Blazevski, Jana
AU  - Momcilović, Miljana
AU  - Mostarica-Stojković, Marija
AU  - Miljković, Djordje
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/654
AB  - CXCL12 plays a protective role in CNS autoimmunity. Expression of CXCL12-gamma, which has distinct structural and functional properties than the other isoforms of CXCL12, was determined in spinal cords of rats immunized to develop experimental autoimmune encephalomyelitis (EAE). CNS expression of CXCL12-gamma was markedly lower in EAE-prone Dark Agouti rats than in EAE-resistant Albino Oxford rats, both in spinal cord homogenates and micro-blood vessels isolated from spinal cords. Inhibition of nitric oxide (NO) synthesis in DA rats upregulated, while donation of NO in AO rats downregulated CNS expression of CXCL12-gamma. NO inhibited CXCL12-gamma expression in astrocytes in vitro. A splice variant of CXCL12-gamma which migrates into nucleolus was not detected in spinal cord or astrocytes. Thus, CXCL12-gamma is expressed in the CNS after EAE induction, but its expression is markedly suppressed in spinal cord affected with full blown inflammation. NO is an important regulator of CXCL12-gamma expression in neuroinflammation.
PB  - Elsevier Science Bv, Amsterdam
T2  - Brain Research
T1  - CXCL12-gamma expression is inhibited in neuroinflammation
EP  - 126
SP  - 120
VL  - 1519
DO  - 10.1016/j.brainres.2013.04.056
ER  - 

@article{
author = "Timotijević, Gordana and Petković, Filip and Blazevski, Jana and Momcilović, Miljana and Mostarica-Stojković, Marija and Miljković, Djordje",
year = "2013",
abstract = "CXCL12 plays a protective role in CNS autoimmunity. Expression of CXCL12-gamma, which has distinct structural and functional properties than the other isoforms of CXCL12, was determined in spinal cords of rats immunized to develop experimental autoimmune encephalomyelitis (EAE). CNS expression of CXCL12-gamma was markedly lower in EAE-prone Dark Agouti rats than in EAE-resistant Albino Oxford rats, both in spinal cord homogenates and micro-blood vessels isolated from spinal cords. Inhibition of nitric oxide (NO) synthesis in DA rats upregulated, while donation of NO in AO rats downregulated CNS expression of CXCL12-gamma. NO inhibited CXCL12-gamma expression in astrocytes in vitro. A splice variant of CXCL12-gamma which migrates into nucleolus was not detected in spinal cord or astrocytes. Thus, CXCL12-gamma is expressed in the CNS after EAE induction, but its expression is markedly suppressed in spinal cord affected with full blown inflammation. NO is an important regulator of CXCL12-gamma expression in neuroinflammation.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Brain Research",
title = "CXCL12-gamma expression is inhibited in neuroinflammation",
pages = "126-120",
volume = "1519",
doi = "10.1016/j.brainres.2013.04.056"
}

Timotijević, G., Petković, F., Blazevski, J., Momcilović, M., Mostarica-Stojković, M.,& Miljković, D.. (2013). CXCL12-gamma expression is inhibited in neuroinflammation. in Brain Research
Elsevier Science Bv, Amsterdam., 1519, 120-126.
https://doi.org/10.1016/j.brainres.2013.04.056

Timotijević G, Petković F, Blazevski J, Momcilović M, Mostarica-Stojković M, Miljković D. CXCL12-gamma expression is inhibited in neuroinflammation. in Brain Research. 2013;1519:120-126.
doi:10.1016/j.brainres.2013.04.056 .

Timotijević, Gordana, Petković, Filip, Blazevski, Jana, Momcilović, Miljana, Mostarica-Stojković, Marija, Miljković, Djordje, "CXCL12-gamma expression is inhibited in neuroinflammation" in Brain Research, 1519 (2013):120-126,
https://doi.org/10.1016/j.brainres.2013.04.056 . .

TY  - JOUR
AU  - Petković, Filip
AU  - Blazevski, Jana
AU  - Momcilović, Miljana
AU  - Timotijević, Gordana
AU  - Zocca, Mai-Britt
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Mangano, Katia
AU  - Fagone, Paolo
AU  - Stošić-Grujičić, Stanislava
AU  - Nicoletti, Ferdinando
AU  - Miljković, Djordje
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/647
AB  - NO-hybridization of the HIV protease inhibitor Saquinavir generates a new chemical entity named Saq-NO, that retains the anti-viral activity and exerts lower toxicity. We show that Saq-NO inhibited the generation of various cytokines in ConA-stimulated unfractionated murine spleen cells and rat lymph nodes stimulated with ConA as well as in purified CD4(+) T cells in vitro and reduced the circulating levels of cytokines in mice challenged with anti-CD3 antibody. Furthermore, Saq-NO reduced IL-17 and IFN-gamma production in myelin basic protein (MBP)-specific cells isolated from rats immunized with MBP. These findings translated well into the in vivo setting as Saq-NO ameliorated the course of the disease in two preclinical models of multiple sclerosis. Our results demonstrate that Saq-NO exerts immunomodulatory effects that warrant studies on its application in autoimmune diseases.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Neuroimmunology
T1  - Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis
EP  - 65
IS  - 1-2
SP  - 55
VL  - 259
DO  - 10.1016/j.jneuroim.2013.03.010
ER  - 

@article{
author = "Petković, Filip and Blazevski, Jana and Momcilović, Miljana and Timotijević, Gordana and Zocca, Mai-Britt and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Mangano, Katia and Fagone, Paolo and Stošić-Grujičić, Stanislava and Nicoletti, Ferdinando and Miljković, Djordje",
year = "2013",
abstract = "NO-hybridization of the HIV protease inhibitor Saquinavir generates a new chemical entity named Saq-NO, that retains the anti-viral activity and exerts lower toxicity. We show that Saq-NO inhibited the generation of various cytokines in ConA-stimulated unfractionated murine spleen cells and rat lymph nodes stimulated with ConA as well as in purified CD4(+) T cells in vitro and reduced the circulating levels of cytokines in mice challenged with anti-CD3 antibody. Furthermore, Saq-NO reduced IL-17 and IFN-gamma production in myelin basic protein (MBP)-specific cells isolated from rats immunized with MBP. These findings translated well into the in vivo setting as Saq-NO ameliorated the course of the disease in two preclinical models of multiple sclerosis. Our results demonstrate that Saq-NO exerts immunomodulatory effects that warrant studies on its application in autoimmune diseases.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis",
pages = "65-55",
number = "1-2",
volume = "259",
doi = "10.1016/j.jneuroim.2013.03.010"
}

Petković, F., Blazevski, J., Momcilović, M., Timotijević, G., Zocca, M., Mijatović, S., Maksimović-Ivanić, D., Mangano, K., Fagone, P., Stošić-Grujičić, S., Nicoletti, F.,& Miljković, D.. (2013). Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology
Elsevier Science Bv, Amsterdam., 259(1-2), 55-65.
https://doi.org/10.1016/j.jneuroim.2013.03.010

Petković F, Blazevski J, Momcilović M, Timotijević G, Zocca M, Mijatović S, Maksimović-Ivanić D, Mangano K, Fagone P, Stošić-Grujičić S, Nicoletti F, Miljković D. Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology. 2013;259(1-2):55-65.
doi:10.1016/j.jneuroim.2013.03.010 .

Petković, Filip, Blazevski, Jana, Momcilović, Miljana, Timotijević, Gordana, Zocca, Mai-Britt, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Mangano, Katia, Fagone, Paolo, Stošić-Grujičić, Stanislava, Nicoletti, Ferdinando, Miljković, Djordje, "Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis" in Journal of Neuroimmunology, 259, no. 1-2 (2013):55-65,
https://doi.org/10.1016/j.jneuroim.2013.03.010 . .