TY  - JOUR
AU  - Pavlović, Radoslav Z.
AU  - Finnegan, Tyler J.
AU  - Metlushko, Anna
AU  - Hansen, Alexandar L.
AU  - Waudby, Christopher A.
AU  - Wang, Xiuze
AU  - Hoefer, Nicole
AU  - McComb, David W.
AU  - Pavić, Aleksandar
AU  - Plackić, Nikola
AU  - Novaković, Jovana
AU  - Bradić, Jovana
AU  - Jeremić, Nevena
AU  - Jakovljević, Vladimir
AU  - Šmit, Biljana
AU  - Matić, Sanja
AU  - Alvarez-Saavedra, Matias A.
AU  - Čapo, Ivan
AU  - Moore, Curtis E.
AU  - Stupp, Samuel I.
AU  - Badjić, Jovica D.
PY  - 2023
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.202303374
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2280
AB  - We describe the preparation, dynamic, assembly characteristics of vase-shaped basket 13− along with its ability to form an inclusion complex with anticancer drug mitoxantrone in abiotic and biotic systems. This novel cavitand has a deep nonpolar pocket consisting of three naphthalimide sides fused to a bicyclic platform at the bottom while carrying polar glycines at the top. The results of 1H Nuclear Magnetic Resonance (NMR), 1H NMR Chemical Exchange Saturation Transfer (CEST), Calorimetry, Hybrid Replica Exchange Molecular Dynamics (REMD), and Microcrystal Electron Diffraction (MicroED) measurements are in line with 1 forming dimer [12]6−, to be in equilibrium with monomers 1(R)3− (relaxed) and 1(S)3− (squeezed). Through simultaneous line-shape analysis of 1H NMR data, kinetic and thermodynamic parameters characterizing these equilibria were quantified. Basket 1(R)3− includes anticancer drug mitoxantrone (MTO2+) in its pocket to give stable binary complex [MTO⊂1]− (Kd=2.1 μM) that can be precipitated in vitro with UV light or pH as stimuli. Both in vitro and in vivo studies showed that the basket is nontoxic, while at a higher proportion with respect to MTO it reduced its cytotoxicity in vitro. With well-characterized internal dynamics and dimerization, the ability to include mitoxantrone, and biocompatibility, the stage is set to develop sequestering agents from deep-cavity baskets.
T2  - Chemistry – A European Journal
T1  - Dynamic and Assembly Characteristics of Deep-Cavity Basket Acting as a Host for Inclusion Complexation of Mitoxantrone in Biotic and Abiotic Systems
IS  - 68
SP  - e202303374
VL  - 29
DO  - 10.1002/chem.202303374
ER  - 

@article{
author = "Pavlović, Radoslav Z. and Finnegan, Tyler J. and Metlushko, Anna and Hansen, Alexandar L. and Waudby, Christopher A. and Wang, Xiuze and Hoefer, Nicole and McComb, David W. and Pavić, Aleksandar and Plackić, Nikola and Novaković, Jovana and Bradić, Jovana and Jeremić, Nevena and Jakovljević, Vladimir and Šmit, Biljana and Matić, Sanja and Alvarez-Saavedra, Matias A. and Čapo, Ivan and Moore, Curtis E. and Stupp, Samuel I. and Badjić, Jovica D.",
year = "2023",
abstract = "We describe the preparation, dynamic, assembly characteristics of vase-shaped basket 13− along with its ability to form an inclusion complex with anticancer drug mitoxantrone in abiotic and biotic systems. This novel cavitand has a deep nonpolar pocket consisting of three naphthalimide sides fused to a bicyclic platform at the bottom while carrying polar glycines at the top. The results of 1H Nuclear Magnetic Resonance (NMR), 1H NMR Chemical Exchange Saturation Transfer (CEST), Calorimetry, Hybrid Replica Exchange Molecular Dynamics (REMD), and Microcrystal Electron Diffraction (MicroED) measurements are in line with 1 forming dimer [12]6−, to be in equilibrium with monomers 1(R)3− (relaxed) and 1(S)3− (squeezed). Through simultaneous line-shape analysis of 1H NMR data, kinetic and thermodynamic parameters characterizing these equilibria were quantified. Basket 1(R)3− includes anticancer drug mitoxantrone (MTO2+) in its pocket to give stable binary complex [MTO⊂1]− (Kd=2.1 μM) that can be precipitated in vitro with UV light or pH as stimuli. Both in vitro and in vivo studies showed that the basket is nontoxic, while at a higher proportion with respect to MTO it reduced its cytotoxicity in vitro. With well-characterized internal dynamics and dimerization, the ability to include mitoxantrone, and biocompatibility, the stage is set to develop sequestering agents from deep-cavity baskets.",
journal = "Chemistry – A European Journal",
title = "Dynamic and Assembly Characteristics of Deep-Cavity Basket Acting as a Host for Inclusion Complexation of Mitoxantrone in Biotic and Abiotic Systems",
number = "68",
pages = "e202303374",
volume = "29",
doi = "10.1002/chem.202303374"
}

Pavlović, R. Z., Finnegan, T. J., Metlushko, A., Hansen, A. L., Waudby, C. A., Wang, X., Hoefer, N., McComb, D. W., Pavić, A., Plackić, N., Novaković, J., Bradić, J., Jeremić, N., Jakovljević, V., Šmit, B., Matić, S., Alvarez-Saavedra, M. A., Čapo, I., Moore, C. E., Stupp, S. I.,& Badjić, J. D.. (2023). Dynamic and Assembly Characteristics of Deep-Cavity Basket Acting as a Host for Inclusion Complexation of Mitoxantrone in Biotic and Abiotic Systems. in Chemistry – A European Journal, 29(68), e202303374.
https://doi.org/10.1002/chem.202303374

Pavlović RZ, Finnegan TJ, Metlushko A, Hansen AL, Waudby CA, Wang X, Hoefer N, McComb DW, Pavić A, Plackić N, Novaković J, Bradić J, Jeremić N, Jakovljević V, Šmit B, Matić S, Alvarez-Saavedra MA, Čapo I, Moore CE, Stupp SI, Badjić JD. Dynamic and Assembly Characteristics of Deep-Cavity Basket Acting as a Host for Inclusion Complexation of Mitoxantrone in Biotic and Abiotic Systems. in Chemistry – A European Journal. 2023;29(68):e202303374.
doi:10.1002/chem.202303374 .

Pavlović, Radoslav Z., Finnegan, Tyler J., Metlushko, Anna, Hansen, Alexandar L., Waudby, Christopher A., Wang, Xiuze, Hoefer, Nicole, McComb, David W., Pavić, Aleksandar, Plackić, Nikola, Novaković, Jovana, Bradić, Jovana, Jeremić, Nevena, Jakovljević, Vladimir, Šmit, Biljana, Matić, Sanja, Alvarez-Saavedra, Matias A., Čapo, Ivan, Moore, Curtis E., Stupp, Samuel I., Badjić, Jovica D., "Dynamic and Assembly Characteristics of Deep-Cavity Basket Acting as a Host for Inclusion Complexation of Mitoxantrone in Biotic and Abiotic Systems" in Chemistry – A European Journal, 29, no. 68 (2023):e202303374,
https://doi.org/10.1002/chem.202303374 . .

TY  - CHAP
AU  - Stevanović, Bojana
AU  - Japundžić-Žigon, Nina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2249
AB  - Vazopresin ili antidiuretički hormon je peptidni molekul koji na vrlo složen način utiče na kardivovaskularnu
i osmotsku homeostazu. Glavni izvori vazopresina su supraoptičko i paraventrikularno jedro hipotalamusa.
Paraventrikularno jedro je ključna centralna stuktura u neuralnoj i endokrinoj regulaciji krvnog pritiska. Sastoji
se od dve funkcionalne celine – neuroendokrine (sekretorne) i autonomne, koje su integralno
povezane u modulaciji kardiovaskularnog odgovora na različite fiziološke zahteve. Neuroendokrini magnocelularni
neuroni sintetišu i oslobađaju vazopresin u cirkulaciju, gde vazopresin ispoljava svoje hormonsko
dejstvo. Jedan deo sintetisanog vazopresina oslobađa se i lokalno, unutar samog jedra, delujući kao
neurotrasmiter/neuromodulator i utičući na signalizaciju okolnih neurona. Manja grupacija parvocelularnih
neurona učestvuje u sekretornom odgovoru paraventrikularnog jedra na različite stresore, menjajujući
aktivnost hipotalamo-hipofizno-nadbubrežne osovine. Autonomni deo paraventrikularnog jedra
dire-ktno ili indirektno reguliše simpatičku aktivnost usmerenu ka kardiorenalnom sistemu. Brojne studije
na životinjama i ljudima ukazuju na važnu vezu između nivoa sinteze vazopresina i nastanka hipertenzije.
Različiti molekularno-biološki pristupi korišćeni su za procenu i izučavanje uticaja promene ekspresije i
strukture centralnih i perifernih vazopresinskih receptora na krvni pritisak. Budući da je hipertenzija oboljenje
sa izraženom incidencom u humanoj populaciji i da povećava rizik od razvoja kardiovaskularnih
komplikacija visokog mortaliteta, od presudnog je značaja pronaći uzrok njenog nastanka. Rasvetljivanje
mehanizama kojima vazopresinski sistem doprinosi genezi hipertenzije imalo bi veliki biomedicinski značaj
i omogućilo bi razvoj antihipertenzivnih lekova nove generacije veće efikasnosti.
AB  - Vasopressin or antidiuretic hormone is a peptide molecule that regulates osmotic and cardiovascular
homeostasis in a complex manner. The main sources of vasopressin are the supraoptic and paraventricular
nucleus of the hypothalamus. Paraventricular nucleus is the pivotal central structure involved in neural
and endocrine regulation of blood pressure. It is composed of two functionally separate compartments
– neuroendocrine (secretory) and autonomic, which integratively modulate cardiovascular response according
to various physiological demands. Neuroendocrine magnocellular neurons are responsible for vasopressin
synthesis and its secretion into the bloodstream, where vasopressin exerts hormonal effects.
Some portion of synthesized vasopressin is released locally within the nucleus itself, acting as neurotransmitter/
neuromodulator and altering signalization of the surrounding neurons. Small amount of parvocellular
neurons is involved in secretory response of the paraventricular nucleus to various stressors,
changing the activity of hypothalamo-hypophyseal-adrenal axis. Autonomic part of the paraventricular nucleus
directly or indirectly regulates the sympathetic charge toward the cardiorenal system. Numerous
animal and human experimental studies indicate the important connection between vasopressin levels
and the development of hypertension. Various genetic approaches have been used to determine and
study the influence of expressional and structural changes in central and peripheral vasopressin receptors
on blood pressure. Since hypertension shows high incidence in the human population and increases a
risk for high mortality cardiovascular complications, it is essential to understand what causes it. Elucidating
the mechanism behind vasopressin contribution to generate hypertension would have a significant
biomedical impact and it would ensure the development of new generation antihypertensive drugs with
increased efficacy.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
T2  - Trendovi u molekularnoj Biologiji
T1  - Uloga vazopresinskog sistema paraventrikularnog jedra u razvoju hipertenzije
EP  - 105
IS  - 3
SP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2249
ER  - 

@inbook{
author = "Stevanović, Bojana and Japundžić-Žigon, Nina",
year = "2023",
abstract = "Vazopresin ili antidiuretički hormon je peptidni molekul koji na vrlo složen način utiče na kardivovaskularnu
i osmotsku homeostazu. Glavni izvori vazopresina su supraoptičko i paraventrikularno jedro hipotalamusa.
Paraventrikularno jedro je ključna centralna stuktura u neuralnoj i endokrinoj regulaciji krvnog pritiska. Sastoji
se od dve funkcionalne celine – neuroendokrine (sekretorne) i autonomne, koje su integralno
povezane u modulaciji kardiovaskularnog odgovora na različite fiziološke zahteve. Neuroendokrini magnocelularni
neuroni sintetišu i oslobađaju vazopresin u cirkulaciju, gde vazopresin ispoljava svoje hormonsko
dejstvo. Jedan deo sintetisanog vazopresina oslobađa se i lokalno, unutar samog jedra, delujući kao
neurotrasmiter/neuromodulator i utičući na signalizaciju okolnih neurona. Manja grupacija parvocelularnih
neurona učestvuje u sekretornom odgovoru paraventrikularnog jedra na različite stresore, menjajujući
aktivnost hipotalamo-hipofizno-nadbubrežne osovine. Autonomni deo paraventrikularnog jedra
dire-ktno ili indirektno reguliše simpatičku aktivnost usmerenu ka kardiorenalnom sistemu. Brojne studije
na životinjama i ljudima ukazuju na važnu vezu između nivoa sinteze vazopresina i nastanka hipertenzije.
Različiti molekularno-biološki pristupi korišćeni su za procenu i izučavanje uticaja promene ekspresije i
strukture centralnih i perifernih vazopresinskih receptora na krvni pritisak. Budući da je hipertenzija oboljenje
sa izraženom incidencom u humanoj populaciji i da povećava rizik od razvoja kardiovaskularnih
komplikacija visokog mortaliteta, od presudnog je značaja pronaći uzrok njenog nastanka. Rasvetljivanje
mehanizama kojima vazopresinski sistem doprinosi genezi hipertenzije imalo bi veliki biomedicinski značaj
i omogućilo bi razvoj antihipertenzivnih lekova nove generacije veće efikasnosti., Vasopressin or antidiuretic hormone is a peptide molecule that regulates osmotic and cardiovascular
homeostasis in a complex manner. The main sources of vasopressin are the supraoptic and paraventricular
nucleus of the hypothalamus. Paraventricular nucleus is the pivotal central structure involved in neural
and endocrine regulation of blood pressure. It is composed of two functionally separate compartments
– neuroendocrine (secretory) and autonomic, which integratively modulate cardiovascular response according
to various physiological demands. Neuroendocrine magnocellular neurons are responsible for vasopressin
synthesis and its secretion into the bloodstream, where vasopressin exerts hormonal effects.
Some portion of synthesized vasopressin is released locally within the nucleus itself, acting as neurotransmitter/
neuromodulator and altering signalization of the surrounding neurons. Small amount of parvocellular
neurons is involved in secretory response of the paraventricular nucleus to various stressors,
changing the activity of hypothalamo-hypophyseal-adrenal axis. Autonomic part of the paraventricular nucleus
directly or indirectly regulates the sympathetic charge toward the cardiorenal system. Numerous
animal and human experimental studies indicate the important connection between vasopressin levels
and the development of hypertension. Various genetic approaches have been used to determine and
study the influence of expressional and structural changes in central and peripheral vasopressin receptors
on blood pressure. Since hypertension shows high incidence in the human population and increases a
risk for high mortality cardiovascular complications, it is essential to understand what causes it. Elucidating
the mechanism behind vasopressin contribution to generate hypertension would have a significant
biomedical impact and it would ensure the development of new generation antihypertensive drugs with
increased efficacy.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
booktitle = "Uloga vazopresinskog sistema paraventrikularnog jedra u razvoju hipertenzije",
pages = "105-90",
number = "3",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2249"
}

Stevanović, B.,& Japundžić-Žigon, N.. (2023). Uloga vazopresinskog sistema paraventrikularnog jedra u razvoju hipertenzije. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo.(3), 90-105.
https://hdl.handle.net/21.15107/rcub_imagine_2249

Stevanović B, Japundžić-Žigon N. Uloga vazopresinskog sistema paraventrikularnog jedra u razvoju hipertenzije. in Trendovi u molekularnoj Biologiji. 2023;(3):90-105.
https://hdl.handle.net/21.15107/rcub_imagine_2249 .

Stevanović, Bojana, Japundžić-Žigon, Nina, "Uloga vazopresinskog sistema paraventrikularnog jedra u razvoju hipertenzije" in Trendovi u molekularnoj Biologiji, no. 3 (2023):90-105,
https://hdl.handle.net/21.15107/rcub_imagine_2249 .

TY  - DATA
AU  - Kabić, Jovana
AU  - Novović, Katarina
AU  - Kekić, Dušan
AU  - Trudić, Anika
AU  - Opavski, Nataša
AU  - Dimkić, Ivica
AU  - Jovčić, Branko
AU  - Gajić, Ina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1785
T2  - Computational and Structural Biotechnology Journal
T2  - Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal
T1  - Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1785
ER  - 

@misc{
author = "Kabić, Jovana and Novović, Katarina and Kekić, Dušan and Trudić, Anika and Opavski, Nataša and Dimkić, Ivica and Jovčić, Branko and Gajić, Ina",
year = "2023",
journal = "Computational and Structural Biotechnology Journal, Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal",
title = "Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1785"
}

Kabić, J., Novović, K., Kekić, D., Trudić, A., Opavski, N., Dimkić, I., Jovčić, B.,& Gajić, I.. (2023). Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045. in Computational and Structural Biotechnology Journal.
https://hdl.handle.net/21.15107/rcub_imagine_1785

Kabić J, Novović K, Kekić D, Trudić A, Opavski N, Dimkić I, Jovčić B, Gajić I. Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045. in Computational and Structural Biotechnology Journal. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1785 .

Kabić, Jovana, Novović, Katarina, Kekić, Dušan, Trudić, Anika, Opavski, Nataša, Dimkić, Ivica, Jovčić, Branko, Gajić, Ina, "Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045" in Computational and Structural Biotechnology Journal (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1785 .

TY  - JOUR
AU  - Kabić, Jovana
AU  - Novović, Katarina
AU  - Kekić, Dušan
AU  - Trudić, Anika
AU  - Opavski, Nataša
AU  - Dimkić, Ivica
AU  - Jovčić, Branko
AU  - Gajić, Ina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S200103702200602X
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1784
AB  - This study aimed to investigate the prevalence and resistance mechanisms of colistin-resistant Acinetobacter baumannii (ColRAB) isolates in Serbia, assess their genetic relatedness to other circulating A. baumannii isolates in the neighbouring European countries, and analyse the global genomic epidemiology of ColRAB isolates. A total of 784 isolates of A. baumannii were recovered from hospitalised patients in Serbia between 2018 and 2021. The antimicrobial susceptibility testing was performed using disk diffusion and broth microdilution. All ColRAB isolates were subjected to DNA isolation and whole-genome sequencing (WGS). Overall, 3.94 % (n = 30) isolates were confirmed as ColRAB. Results of mutational and transcriptional analysis of genes associated with colistin resistance indicate the central role of the two-component regulating system, PmrAB, and increased expression of the pmrC gene in ColRAB. Most of the isolates (n = 29, 96.6 %) belonged to international clone II, with the most common sequence type being STPas2 (n = 23, 76.6 %). Based on the WGS analysis, ColRAB isolates belonging to the same ST isolated in various countries were grouped into the same clusters, indicating the global dissemination of several high-risk clonal lineages. Phylogenomic analysis of ColRAB isolates, together with all previously published A. baumannii genomes from South-Eastern European countries, showed that colistin resistance arose independently in several clonal lineages. Comparative genomic analysis revealed multiple genes with various roles (transcriptional regulation, transmembrane transport, outer membrane assembly, etc.), which might be associated with colistin resistance in A. baumannii. The obtained findings serve as the basis for further studies, contributing to a better understanding of colistin resistance mechanisms in A. baumannii.
T2  - Computational and Structural Biotechnology Journal
T2  - Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal
T1  - Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii
EP  - 585
SP  - 574
VL  - 21
DO  - 10.1016/j.csbj.2022.12.045
DO  - 10.1016/j.csbj.2022.12.045
ER  - 

@article{
author = "Kabić, Jovana and Novović, Katarina and Kekić, Dušan and Trudić, Anika and Opavski, Nataša and Dimkić, Ivica and Jovčić, Branko and Gajić, Ina",
year = "2023",
abstract = "This study aimed to investigate the prevalence and resistance mechanisms of colistin-resistant Acinetobacter baumannii (ColRAB) isolates in Serbia, assess their genetic relatedness to other circulating A. baumannii isolates in the neighbouring European countries, and analyse the global genomic epidemiology of ColRAB isolates. A total of 784 isolates of A. baumannii were recovered from hospitalised patients in Serbia between 2018 and 2021. The antimicrobial susceptibility testing was performed using disk diffusion and broth microdilution. All ColRAB isolates were subjected to DNA isolation and whole-genome sequencing (WGS). Overall, 3.94 % (n = 30) isolates were confirmed as ColRAB. Results of mutational and transcriptional analysis of genes associated with colistin resistance indicate the central role of the two-component regulating system, PmrAB, and increased expression of the pmrC gene in ColRAB. Most of the isolates (n = 29, 96.6 %) belonged to international clone II, with the most common sequence type being STPas2 (n = 23, 76.6 %). Based on the WGS analysis, ColRAB isolates belonging to the same ST isolated in various countries were grouped into the same clusters, indicating the global dissemination of several high-risk clonal lineages. Phylogenomic analysis of ColRAB isolates, together with all previously published A. baumannii genomes from South-Eastern European countries, showed that colistin resistance arose independently in several clonal lineages. Comparative genomic analysis revealed multiple genes with various roles (transcriptional regulation, transmembrane transport, outer membrane assembly, etc.), which might be associated with colistin resistance in A. baumannii. The obtained findings serve as the basis for further studies, contributing to a better understanding of colistin resistance mechanisms in A. baumannii.",
journal = "Computational and Structural Biotechnology Journal, Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal",
title = "Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii",
pages = "585-574",
volume = "21",
doi = "10.1016/j.csbj.2022.12.045, 10.1016/j.csbj.2022.12.045"
}

Kabić, J., Novović, K., Kekić, D., Trudić, A., Opavski, N., Dimkić, I., Jovčić, B.,& Gajić, I.. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. in Computational and Structural Biotechnology Journal, 21, 574-585.
https://doi.org/10.1016/j.csbj.2022.12.045

Kabić J, Novović K, Kekić D, Trudić A, Opavski N, Dimkić I, Jovčić B, Gajić I. Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. in Computational and Structural Biotechnology Journal. 2023;21:574-585.
doi:10.1016/j.csbj.2022.12.045 .

Kabić, Jovana, Novović, Katarina, Kekić, Dušan, Trudić, Anika, Opavski, Nataša, Dimkić, Ivica, Jovčić, Branko, Gajić, Ina, "Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii" in Computational and Structural Biotechnology Journal, 21 (2023):574-585,
https://doi.org/10.1016/j.csbj.2022.12.045 . .

TY  - CONF
AU  - Kosić, Marija
AU  - Nešić, Zorica
AU  - Žigon, Nina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2256
AB  - Doksorubicin je efikasan lek u terapiji brojnih maligniteta. Međutim, njegovu upotrebu umnogome ograničava
nastanak kardiotoksičnosti, koja može dovesti do kongestivne srčane insuficijencije. Mehanizmi
doksorubicinske kardiotoksičnosti nisu sasvim razjašnjeni, a kada dođe do kongestivne srčane insuficijencije,
stopa smrtnosti je približno 50%. Iako specifična terapija ne postoji, efikasnost su pokazali antagonisti
beta-adrenergičkih receptora (β-AR). U srčanoj insuficijenciji je pokazano da kompenzatorno
povećan tonus simpatikusa i cirkulišućih kateholamina dovodi do selektivne nishodne regulacije β1-AR,
što dalje smanjuje kontraktilnu snagu srca. U procesima desenzitizacije i nishodne regulacije β-AR, važnu
ulogu imaju kinaze G protein-spregnutih receptora (GRK) i arestini. Utvrđeno je da je GRK2 ushodno regulisana
u nekim eksperimentalnim modelima srčane insuficijencije i da njena inhibicija sprečava štetno
remodelovanje srčanog tkiva. Ovaj rad daje pregled rezultata istraživanja β-AR i procesa njihove nishodne
regulacije, kao i uticaja inhibicije GRK2 na remodelovanje srca u kardiomiopatiji izazvanoj doksorubicinom.
AB  - Doxorubicin is an effective drug for the treatment of numerous malignancies. However, its use is greatly
hampered by the occurrence of cardiotoxicity, which can lead to congestive heart failure. The mechanisms
of doxorubicin cardiotoxicity are not fully understood, and when congestive heart failure occurs, the mortality
rate is approximately 50%. Although there is no specific treatment for doxorubicin cardiotoxicity,
beta-adrenergic receptor (β-AR) antagonists have limited efficacy. In heart failure, compensatory increased
sympathetic tone and circulating catecholamines lead to selective downregulation of β1-AR, which
further reduces the contractile force of the heart. G protein-coupled receptor kinases (GRKs) and arrestins
play an important role in the processes of desensitization and downregulation of β-AR. GRK2 has been
found to be up-regulated in some experimental models of heart failure and its inhibition prevents harmful
remodeling of cardiac tissue. This paper provides the findings on β-AR and the process of their downregulation,
as well as on the effects of GRK2 inhibition on cardiac remodeling in doxorubicin-induced
cardiomyopathy.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
C3  - Trendovi u molekularnoj Biologiji
T1  - Beta-adrenergički receptori i kinaze uključene u proces njihove nishodne regulacije u eksperimentalnom modelu kardiomiopatije izazvane doksorubicinom
T1  - Beta-adrenergic receptors and kinases involved in the process of their downregulation in experimental model of cardiomyopathy induced by doxorubicin
EP  - 231
IS  - 3
SP  - 218
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2256
ER  - 

@conference{
author = "Kosić, Marija and Nešić, Zorica and Žigon, Nina",
year = "2023",
abstract = "Doksorubicin je efikasan lek u terapiji brojnih maligniteta. Međutim, njegovu upotrebu umnogome ograničava
nastanak kardiotoksičnosti, koja može dovesti do kongestivne srčane insuficijencije. Mehanizmi
doksorubicinske kardiotoksičnosti nisu sasvim razjašnjeni, a kada dođe do kongestivne srčane insuficijencije,
stopa smrtnosti je približno 50%. Iako specifična terapija ne postoji, efikasnost su pokazali antagonisti
beta-adrenergičkih receptora (β-AR). U srčanoj insuficijenciji je pokazano da kompenzatorno
povećan tonus simpatikusa i cirkulišućih kateholamina dovodi do selektivne nishodne regulacije β1-AR,
što dalje smanjuje kontraktilnu snagu srca. U procesima desenzitizacije i nishodne regulacije β-AR, važnu
ulogu imaju kinaze G protein-spregnutih receptora (GRK) i arestini. Utvrđeno je da je GRK2 ushodno regulisana
u nekim eksperimentalnim modelima srčane insuficijencije i da njena inhibicija sprečava štetno
remodelovanje srčanog tkiva. Ovaj rad daje pregled rezultata istraživanja β-AR i procesa njihove nishodne
regulacije, kao i uticaja inhibicije GRK2 na remodelovanje srca u kardiomiopatiji izazvanoj doksorubicinom., Doxorubicin is an effective drug for the treatment of numerous malignancies. However, its use is greatly
hampered by the occurrence of cardiotoxicity, which can lead to congestive heart failure. The mechanisms
of doxorubicin cardiotoxicity are not fully understood, and when congestive heart failure occurs, the mortality
rate is approximately 50%. Although there is no specific treatment for doxorubicin cardiotoxicity,
beta-adrenergic receptor (β-AR) antagonists have limited efficacy. In heart failure, compensatory increased
sympathetic tone and circulating catecholamines lead to selective downregulation of β1-AR, which
further reduces the contractile force of the heart. G protein-coupled receptor kinases (GRKs) and arrestins
play an important role in the processes of desensitization and downregulation of β-AR. GRK2 has been
found to be up-regulated in some experimental models of heart failure and its inhibition prevents harmful
remodeling of cardiac tissue. This paper provides the findings on β-AR and the process of their downregulation,
as well as on the effects of GRK2 inhibition on cardiac remodeling in doxorubicin-induced
cardiomyopathy.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
title = "Beta-adrenergički receptori i kinaze uključene u proces njihove nishodne regulacije u eksperimentalnom modelu kardiomiopatije izazvane doksorubicinom, Beta-adrenergic receptors and kinases involved in the process of their downregulation in experimental model of cardiomyopathy induced by doxorubicin",
pages = "231-218",
number = "3",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2256"
}

Kosić, M., Nešić, Z.,& Žigon, N.. (2023). Beta-adrenergički receptori i kinaze uključene u proces njihove nishodne regulacije u eksperimentalnom modelu kardiomiopatije izazvane doksorubicinom. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo.(3), 218-231.
https://hdl.handle.net/21.15107/rcub_imagine_2256

Kosić M, Nešić Z, Žigon N. Beta-adrenergički receptori i kinaze uključene u proces njihove nishodne regulacije u eksperimentalnom modelu kardiomiopatije izazvane doksorubicinom. in Trendovi u molekularnoj Biologiji. 2023;(3):218-231.
https://hdl.handle.net/21.15107/rcub_imagine_2256 .

Kosić, Marija, Nešić, Zorica, Žigon, Nina, "Beta-adrenergički receptori i kinaze uključene u proces njihove nishodne regulacije u eksperimentalnom modelu kardiomiopatije izazvane doksorubicinom" in Trendovi u molekularnoj Biologiji, no. 3 (2023):218-231,
https://hdl.handle.net/21.15107/rcub_imagine_2256 .

TY  - JOUR
AU  - Pajović, Vladislav
AU  - Kovacshazi, Csenger
AU  - Kosić, Marija
AU  - Vasić, Marko
AU  - Dukić, Ljiljana
AU  - Brenner, Gabor B.
AU  - Giricz, Zoltan
AU  - Bajić, Dragana
AU  - Ferdinandy, Peter
AU  - Japundžić-Žigon, Nina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1447
AB  - Cardiomyopathy resistant to treatment is the most serious adverse effect of doxorubicin (dox). The mechanisms of dox-induced cardiomyopathy (DCM) have been extensively studied in dilated forms of DCM. However, efficient treatment did not emerge. The aim of the present work was to revisit the experimental model of DCM in rats, to define phenotype/s and associate them to the changes in cardiac transcriptome. Male Wistar rats equipped with radiotelemetry device, were randomized in DOX group (5 mg/0,5 mL/kg, IV dox; n = 18) and CONT group (0,5 mL/kg IV saline; n = 6). Echocardiography, autonomic spectral markers and baroreceptor reflex evaluation was performed prior to, and after treatment. Blood samples were collected at the end of experimentation. Cardiac, renal and hepatic tissues were analysed post-mortem by histology. Changes in expression of key cardiac genes affected by dox were assessed by RT-qPCR. Phenotypes were identified by clustering non-redundant features using four different algorithms averaged by evidence accumulation cluster technique. The results emphasize the existence of two major phenotypes of DCM with comparably high mortality rates: phenotype 1 characterized by, left ventricular (LV) dilatation, thinning of LV posterior wall, reduced LV ejection fraction (LVEF) and fractional shortening (LVFS), decreased HR variability (HRV), decreased baroreceptor effectiveness index (BEI) and increased NT-proBNP; and phenotype 2 with LV hypertrophy - increased LV mass, preserved LVEF, LVFS, no changes in HRV and BEI and moderate NT-proBNP increase. Both phenotypes exhibited a genetic shift to a new-born program.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Toxicology and Applied Pharmacology
T1  - Phenomapping for classification of doxorubicin-induced cardiomyopathy in rats
VL  - 423
DO  - 10.1016/j.taap.2021.115579
ER  - 

@article{
author = "Pajović, Vladislav and Kovacshazi, Csenger and Kosić, Marija and Vasić, Marko and Dukić, Ljiljana and Brenner, Gabor B. and Giricz, Zoltan and Bajić, Dragana and Ferdinandy, Peter and Japundžić-Žigon, Nina",
year = "2021",
abstract = "Cardiomyopathy resistant to treatment is the most serious adverse effect of doxorubicin (dox). The mechanisms of dox-induced cardiomyopathy (DCM) have been extensively studied in dilated forms of DCM. However, efficient treatment did not emerge. The aim of the present work was to revisit the experimental model of DCM in rats, to define phenotype/s and associate them to the changes in cardiac transcriptome. Male Wistar rats equipped with radiotelemetry device, were randomized in DOX group (5 mg/0,5 mL/kg, IV dox; n = 18) and CONT group (0,5 mL/kg IV saline; n = 6). Echocardiography, autonomic spectral markers and baroreceptor reflex evaluation was performed prior to, and after treatment. Blood samples were collected at the end of experimentation. Cardiac, renal and hepatic tissues were analysed post-mortem by histology. Changes in expression of key cardiac genes affected by dox were assessed by RT-qPCR. Phenotypes were identified by clustering non-redundant features using four different algorithms averaged by evidence accumulation cluster technique. The results emphasize the existence of two major phenotypes of DCM with comparably high mortality rates: phenotype 1 characterized by, left ventricular (LV) dilatation, thinning of LV posterior wall, reduced LV ejection fraction (LVEF) and fractional shortening (LVFS), decreased HR variability (HRV), decreased baroreceptor effectiveness index (BEI) and increased NT-proBNP; and phenotype 2 with LV hypertrophy - increased LV mass, preserved LVEF, LVFS, no changes in HRV and BEI and moderate NT-proBNP increase. Both phenotypes exhibited a genetic shift to a new-born program.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Toxicology and Applied Pharmacology",
title = "Phenomapping for classification of doxorubicin-induced cardiomyopathy in rats",
volume = "423",
doi = "10.1016/j.taap.2021.115579"
}

Pajović, V., Kovacshazi, C., Kosić, M., Vasić, M., Dukić, L., Brenner, G. B., Giricz, Z., Bajić, D., Ferdinandy, P.,& Japundžić-Žigon, N.. (2021). Phenomapping for classification of doxorubicin-induced cardiomyopathy in rats. in Toxicology and Applied Pharmacology
Academic Press Inc Elsevier Science, San Diego., 423.
https://doi.org/10.1016/j.taap.2021.115579

Pajović V, Kovacshazi C, Kosić M, Vasić M, Dukić L, Brenner GB, Giricz Z, Bajić D, Ferdinandy P, Japundžić-Žigon N. Phenomapping for classification of doxorubicin-induced cardiomyopathy in rats. in Toxicology and Applied Pharmacology. 2021;423.
doi:10.1016/j.taap.2021.115579 .

Pajović, Vladislav, Kovacshazi, Csenger, Kosić, Marija, Vasić, Marko, Dukić, Ljiljana, Brenner, Gabor B., Giricz, Zoltan, Bajić, Dragana, Ferdinandy, Peter, Japundžić-Žigon, Nina, "Phenomapping for classification of doxorubicin-induced cardiomyopathy in rats" in Toxicology and Applied Pharmacology, 423 (2021),
https://doi.org/10.1016/j.taap.2021.115579 . .