TY  - JOUR
AU  - Novaković, Miroslav
AU  - Bukvicki, Danka
AU  - Anđelković, Boban
AU  - Ilić-Tomić, Tatjana
AU  - Veljić, Milan
AU  - Tešević, Vele
AU  - Asakawa, Yoshinori
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1281
AB  - Seven new bisbibenzyls (1-7) were isolated from the methanol extract of the liverwort Lunularia cruciata along with one previously known bibenzyl and five known bisbibenzyls. The structures of compounds 1-7 were elucidated on the basis of the spectroscopic data, These newly isolated bisbibenzyls may be divided into two groups, the acyclic bisbibenzyls, perrottetins (1-3), and the cyclic analogues, riccardins (4-7). Besides standard perrottetin and riccardin structures (1 and 4, respectively), they contain phenanthrene (3 and 5), dihydrophenanthrene (2), and quinone moieties (6 and 7), rarely found in natural products. The new compounds 3 and 5, as well as the known riccardin G, exhibited cytotoxic activity against the A549 lung cancer cell line with IC50 values of 5.0, 5.0, and 2.5 mu M, respectively.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Natural Products
T1  - Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia cruciata
EP  - 701
IS  - 4
SP  - 694
VL  - 82
DO  - 10.1021/acs.jnatprod.8b00390
ER  - 

@article{
author = "Novaković, Miroslav and Bukvicki, Danka and Anđelković, Boban and Ilić-Tomić, Tatjana and Veljić, Milan and Tešević, Vele and Asakawa, Yoshinori",
year = "2019",
abstract = "Seven new bisbibenzyls (1-7) were isolated from the methanol extract of the liverwort Lunularia cruciata along with one previously known bibenzyl and five known bisbibenzyls. The structures of compounds 1-7 were elucidated on the basis of the spectroscopic data, These newly isolated bisbibenzyls may be divided into two groups, the acyclic bisbibenzyls, perrottetins (1-3), and the cyclic analogues, riccardins (4-7). Besides standard perrottetin and riccardin structures (1 and 4, respectively), they contain phenanthrene (3 and 5), dihydrophenanthrene (2), and quinone moieties (6 and 7), rarely found in natural products. The new compounds 3 and 5, as well as the known riccardin G, exhibited cytotoxic activity against the A549 lung cancer cell line with IC50 values of 5.0, 5.0, and 2.5 mu M, respectively.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Natural Products",
title = "Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia cruciata",
pages = "701-694",
number = "4",
volume = "82",
doi = "10.1021/acs.jnatprod.8b00390"
}

Novaković, M., Bukvicki, D., Anđelković, B., Ilić-Tomić, T., Veljić, M., Tešević, V.,& Asakawa, Y.. (2019). Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia cruciata. in Journal of Natural Products
Amer Chemical Soc, Washington., 82(4), 694-701.
https://doi.org/10.1021/acs.jnatprod.8b00390

Novaković M, Bukvicki D, Anđelković B, Ilić-Tomić T, Veljić M, Tešević V, Asakawa Y. Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia cruciata. in Journal of Natural Products. 2019;82(4):694-701.
doi:10.1021/acs.jnatprod.8b00390 .

Novaković, Miroslav, Bukvicki, Danka, Anđelković, Boban, Ilić-Tomić, Tatjana, Veljić, Milan, Tešević, Vele, Asakawa, Yoshinori, "Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia cruciata" in Journal of Natural Products, 82, no. 4 (2019):694-701,
https://doi.org/10.1021/acs.jnatprod.8b00390 . .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Dodos, Tanja
AU  - Rajcević, Nemanja
AU  - Bojović, Srdjan
AU  - Marin, Petar D.
AU  - Aleksić, Jelena M.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/835
AB  - Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Dordevic; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is - exceptionally high levels of genetic diversity (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans' and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.
PB  - Finnish Soc Forest Science-Natural Resources Inst Finland, Vantaa
T2  - Silva Fennica
T1  - Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data
IS  - 5
VL  - 49
DO  - 10.14214/sf.1415
ER  - 

@article{
author = "Sarac, Zorica and Dodos, Tanja and Rajcević, Nemanja and Bojović, Srdjan and Marin, Petar D. and Aleksić, Jelena M.",
year = "2015",
abstract = "Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Dordevic; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is - exceptionally high levels of genetic diversity (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans' and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.",
publisher = "Finnish Soc Forest Science-Natural Resources Inst Finland, Vantaa",
journal = "Silva Fennica",
title = "Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data",
number = "5",
volume = "49",
doi = "10.14214/sf.1415"
}

Sarac, Z., Dodos, T., Rajcević, N., Bojović, S., Marin, P. D.,& Aleksić, J. M.. (2015). Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data. in Silva Fennica
Finnish Soc Forest Science-Natural Resources Inst Finland, Vantaa., 49(5).
https://doi.org/10.14214/sf.1415

Sarac Z, Dodos T, Rajcević N, Bojović S, Marin PD, Aleksić JM. Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data. in Silva Fennica. 2015;49(5).
doi:10.14214/sf.1415 .

Sarac, Zorica, Dodos, Tanja, Rajcević, Nemanja, Bojović, Srdjan, Marin, Petar D., Aleksić, Jelena M., "Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data" in Silva Fennica, 49, no. 5 (2015),
https://doi.org/10.14214/sf.1415 . .

TY  - JOUR
AU  - Alimpić, Ana
AU  - Kotur, Nikola
AU  - Stanković, Biljana
AU  - Marin, Petar D.
AU  - Matevski, Vlado
AU  - Al Sheef, Najat Beleed
AU  - Duletić-Lausević, Sonja
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/795
AB  - The current paper presents antioxidant and cytotoxic activities and total phenolic and flavonoid content of the selected species of genus Salvia (Lamiaceae) growing wild in Macedonia (S. jurisicii Kosanin, S. amplexicaulis Lam., S. ringens Sibth. & Sm.) and Libya (S. fruticosa Mill. and S. lanigera Poir.). Crude water extracts, obtained from aerial parts, were yielded from 6.50 to 14.32%. Total phenolic content was the highest in water extracts of S. amplexicaulis and S. ringens (226.30 and 189.01 mg GAE/g, respectively), while the flavonoids were the most abundant in S. jurisicii extract (32.36 mg QE/g). Antioxidant activities of extracts were measured using DPPH, ABTS and FRAP assays. S. amplexicaulis and S. ringens extracts showed the strongest antioxidant activity, measured using DPPH (14.21 and 23.44 mu g/mL, respectively) and ABTS assays (2.91 and 2.42 mg AAE/g, respectively). In FRAP assay, S. amplexicaulis and S. fruticosa extracts exhibited strongest activity (1406.73 and 1191.51 mu mol Fe(II)/g). Water extract of S. amplexicaulis and S. ringens performed the strongest cytotoxic activity against K562 cells (151.07 and 173.06 mu g/mL, respectively). Based on these findings, it can be concluded that S. amplexicaulis and S. ringens water extracts could be considered as possible source of antioxidant and cytotoxic agents.
PB  - Druckerei Liddy Halm, Gottingen
T2  - Journal of Applied Botany and Food Quality
T1  - The in vitro antioxidative and cytotoxic effects of selected Salvia species water extracts
EP  - 119
SP  - 115
VL  - 88
DO  - 10.5073/JABFQ.2015.088.016
ER  - 

@article{
author = "Alimpić, Ana and Kotur, Nikola and Stanković, Biljana and Marin, Petar D. and Matevski, Vlado and Al Sheef, Najat Beleed and Duletić-Lausević, Sonja",
year = "2015",
abstract = "The current paper presents antioxidant and cytotoxic activities and total phenolic and flavonoid content of the selected species of genus Salvia (Lamiaceae) growing wild in Macedonia (S. jurisicii Kosanin, S. amplexicaulis Lam., S. ringens Sibth. & Sm.) and Libya (S. fruticosa Mill. and S. lanigera Poir.). Crude water extracts, obtained from aerial parts, were yielded from 6.50 to 14.32%. Total phenolic content was the highest in water extracts of S. amplexicaulis and S. ringens (226.30 and 189.01 mg GAE/g, respectively), while the flavonoids were the most abundant in S. jurisicii extract (32.36 mg QE/g). Antioxidant activities of extracts were measured using DPPH, ABTS and FRAP assays. S. amplexicaulis and S. ringens extracts showed the strongest antioxidant activity, measured using DPPH (14.21 and 23.44 mu g/mL, respectively) and ABTS assays (2.91 and 2.42 mg AAE/g, respectively). In FRAP assay, S. amplexicaulis and S. fruticosa extracts exhibited strongest activity (1406.73 and 1191.51 mu mol Fe(II)/g). Water extract of S. amplexicaulis and S. ringens performed the strongest cytotoxic activity against K562 cells (151.07 and 173.06 mu g/mL, respectively). Based on these findings, it can be concluded that S. amplexicaulis and S. ringens water extracts could be considered as possible source of antioxidant and cytotoxic agents.",
publisher = "Druckerei Liddy Halm, Gottingen",
journal = "Journal of Applied Botany and Food Quality",
title = "The in vitro antioxidative and cytotoxic effects of selected Salvia species water extracts",
pages = "119-115",
volume = "88",
doi = "10.5073/JABFQ.2015.088.016"
}

Alimpić, A., Kotur, N., Stanković, B., Marin, P. D., Matevski, V., Al Sheef, N. B.,& Duletić-Lausević, S.. (2015). The in vitro antioxidative and cytotoxic effects of selected Salvia species water extracts. in Journal of Applied Botany and Food Quality
Druckerei Liddy Halm, Gottingen., 88, 115-119.
https://doi.org/10.5073/JABFQ.2015.088.016

Alimpić A, Kotur N, Stanković B, Marin PD, Matevski V, Al Sheef NB, Duletić-Lausević S. The in vitro antioxidative and cytotoxic effects of selected Salvia species water extracts. in Journal of Applied Botany and Food Quality. 2015;88:115-119.
doi:10.5073/JABFQ.2015.088.016 .

Alimpić, Ana, Kotur, Nikola, Stanković, Biljana, Marin, Petar D., Matevski, Vlado, Al Sheef, Najat Beleed, Duletić-Lausević, Sonja, "The in vitro antioxidative and cytotoxic effects of selected Salvia species water extracts" in Journal of Applied Botany and Food Quality, 88 (2015):115-119,
https://doi.org/10.5073/JABFQ.2015.088.016 . .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Aleksić, Jelena M.
AU  - Dodos, Tanja
AU  - Rajcević, Nemanja
AU  - Bojović, Srdjan
AU  - Marin, Petar D.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/815
AB  - Due to the current lack of nuclear microsatellites (simple sequence repeats SSRs) specifically developed for Pinus nigra, an important European coniferous species, we cross-species amplified 12 EST-SSRs (expressed sequence tagged SSRs) developed for other Pinus species in P. nigra in order to delineate loci which can be used for assessing levels of genetic diversity and genetic structuring in this species. We amplified these loci in individuals from seven populations from the central Balkans representing four recognized infraspecific taxa of P. nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica). Contrary to expectations on high transferability of EST-SSRs into related species, only three out of 12 tested loci were successfully amplified in P. nigra, but they displayed lack/low levels of polymorphism or generated multilocus amplification products. Thus, our estimates on levels of genetic diversity (HE = 0.183) and genetic differentiation (F-ST = 0.007) were based on variability of a single locus harboring four alleles only and they should be taken with cautions. Our study highlights the need for the development of high-resolution molecular markers, such as co-dominant genic or genomic SSRs or predominantly biallelic SNPs, or utilization of anonymous dominant markers, such as AFLPs, for genotyping in P. nigra.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra
EP  - 217
IS  - 1
SP  - 205
VL  - 47
DO  - 10.2298/GENSR1501205S
ER  - 

@article{
author = "Sarac, Zorica and Aleksić, Jelena M. and Dodos, Tanja and Rajcević, Nemanja and Bojović, Srdjan and Marin, Petar D.",
year = "2015",
abstract = "Due to the current lack of nuclear microsatellites (simple sequence repeats SSRs) specifically developed for Pinus nigra, an important European coniferous species, we cross-species amplified 12 EST-SSRs (expressed sequence tagged SSRs) developed for other Pinus species in P. nigra in order to delineate loci which can be used for assessing levels of genetic diversity and genetic structuring in this species. We amplified these loci in individuals from seven populations from the central Balkans representing four recognized infraspecific taxa of P. nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica). Contrary to expectations on high transferability of EST-SSRs into related species, only three out of 12 tested loci were successfully amplified in P. nigra, but they displayed lack/low levels of polymorphism or generated multilocus amplification products. Thus, our estimates on levels of genetic diversity (HE = 0.183) and genetic differentiation (F-ST = 0.007) were based on variability of a single locus harboring four alleles only and they should be taken with cautions. Our study highlights the need for the development of high-resolution molecular markers, such as co-dominant genic or genomic SSRs or predominantly biallelic SNPs, or utilization of anonymous dominant markers, such as AFLPs, for genotyping in P. nigra.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra",
pages = "217-205",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501205S"
}

Sarac, Z., Aleksić, J. M., Dodos, T., Rajcević, N., Bojović, S.,& Marin, P. D.. (2015). Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(1), 205-217.
https://doi.org/10.2298/GENSR1501205S

Sarac Z, Aleksić JM, Dodos T, Rajcević N, Bojović S, Marin PD. Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra. in Genetika-Belgrade. 2015;47(1):205-217.
doi:10.2298/GENSR1501205S .

Sarac, Zorica, Aleksić, Jelena M., Dodos, Tanja, Rajcević, Nemanja, Bojović, Srdjan, Marin, Petar D., "Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra" in Genetika-Belgrade, 47, no. 1 (2015):205-217,
https://doi.org/10.2298/GENSR1501205S . .

TY  - JOUR
AU  - Dodos, Tanja
AU  - Aleksić, Jelena M.
AU  - Rajcević, N.
AU  - Marin, P. D.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/766
AB  - Aromatic species of the genus Satureja are rich in secondary metabolites that interfere with DNA isolation procedures. Four protocols based on the standard CTAB DNA extraction protocol of Doyle and Doyle (1987) were tested in six savory taxa. The polyphenol adsorbents activated charcoal and/or polyvinylpyrrolidone 10 were employed in three procedures (B, C and D); for the elimination of polysaccharides, 4M NaCl was applied in the latter two. The highest DNA yield was obtained with Protocol D and averaged 1420.7 +/- 398.3 mu g DNA/g of dry leaf tissue. Optimal values of the absorbance ratio 260/280 of all DNA solutions revealed the absence or only negligible contamination by proteins. Contamination by polysaccharides inferred from the absorbance ratio 260/230 showed that Protocol C provided the least contaminated material (average of 1.7 +/- 0.4). Enzymatic reactions of DNA solutions obtained by Protocol D showed amplification of both loci in all individuals. In conclusion, Protocol D is suitable for the isolation of high quantities of pure DNA from Satureja spp.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - A robust and cost-effective method for DNA isolation from satureja species (lamiaceae)
EP  - 297
IS  - 1
SP  - 285
VL  - 66
DO  - 10.2298/ABS1401285D
ER  - 

@article{
author = "Dodos, Tanja and Aleksić, Jelena M. and Rajcević, N. and Marin, P. D.",
year = "2014",
abstract = "Aromatic species of the genus Satureja are rich in secondary metabolites that interfere with DNA isolation procedures. Four protocols based on the standard CTAB DNA extraction protocol of Doyle and Doyle (1987) were tested in six savory taxa. The polyphenol adsorbents activated charcoal and/or polyvinylpyrrolidone 10 were employed in three procedures (B, C and D); for the elimination of polysaccharides, 4M NaCl was applied in the latter two. The highest DNA yield was obtained with Protocol D and averaged 1420.7 +/- 398.3 mu g DNA/g of dry leaf tissue. Optimal values of the absorbance ratio 260/280 of all DNA solutions revealed the absence or only negligible contamination by proteins. Contamination by polysaccharides inferred from the absorbance ratio 260/230 showed that Protocol C provided the least contaminated material (average of 1.7 +/- 0.4). Enzymatic reactions of DNA solutions obtained by Protocol D showed amplification of both loci in all individuals. In conclusion, Protocol D is suitable for the isolation of high quantities of pure DNA from Satureja spp.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "A robust and cost-effective method for DNA isolation from satureja species (lamiaceae)",
pages = "297-285",
number = "1",
volume = "66",
doi = "10.2298/ABS1401285D"
}

Dodos, T., Aleksić, J. M., Rajcević, N.,& Marin, P. D.. (2014). A robust and cost-effective method for DNA isolation from satureja species (lamiaceae). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(1), 285-297.
https://doi.org/10.2298/ABS1401285D

Dodos T, Aleksić JM, Rajcević N, Marin PD. A robust and cost-effective method for DNA isolation from satureja species (lamiaceae). in Archives of Biological Sciences. 2014;66(1):285-297.
doi:10.2298/ABS1401285D .

Dodos, Tanja, Aleksić, Jelena M., Rajcević, N., Marin, P. D., "A robust and cost-effective method for DNA isolation from satureja species (lamiaceae)" in Archives of Biological Sciences, 66, no. 1 (2014):285-297,
https://doi.org/10.2298/ABS1401285D . .