TY  - JOUR
AU  - Stanković, Nada
AU  - Šenerović, Lidija
AU  - Bojić-Trbojević, Z.
AU  - Vucković, I.
AU  - Vicovac, L.
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/674
AB  - AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity
EP  - 1306
IS  - 6
SP  - 1297
VL  - 115
DO  - 10.1111/jam.12326
ER  - 

@article{
author = "Stanković, Nada and Šenerović, Lidija and Bojić-Trbojević, Z. and Vucković, I. and Vicovac, L. and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2013",
abstract = "AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity",
pages = "1306-1297",
number = "6",
volume = "115",
doi = "10.1111/jam.12326"
}

Stanković, N., Šenerović, L., Bojić-Trbojević, Z., Vucković, I., Vicovac, L., Vasiljević, B.,& Nikodinović-Runić, J.. (2013). Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology
Wiley, Hoboken., 115(6), 1297-1306.
https://doi.org/10.1111/jam.12326

Stanković N, Šenerović L, Bojić-Trbojević Z, Vucković I, Vicovac L, Vasiljević B, Nikodinović-Runić J. Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology. 2013;115(6):1297-1306.
doi:10.1111/jam.12326 .

Stanković, Nada, Šenerović, Lidija, Bojić-Trbojević, Z., Vucković, I., Vicovac, L., Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity" in Journal of Applied Microbiology, 115, no. 6 (2013):1297-1306,
https://doi.org/10.1111/jam.12326 . .

TY  - JOUR
AU  - Ćujić, Danica
AU  - Bojić-Trbojević, Žanka
AU  - Tošić, Nataša
AU  - Pavlović, Sonja
AU  - Vićovac, Ljiljana
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/689
AB  - Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro
EP  - 1337
IS  - 4
SP  - 1331
VL  - 65
DO  - 10.2298/ABS1304331C
ER  - 

@article{
author = "Ćujić, Danica and Bojić-Trbojević, Žanka and Tošić, Nataša and Pavlović, Sonja and Vićovac, Ljiljana",
year = "2013",
abstract = "Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro",
pages = "1337-1331",
number = "4",
volume = "65",
doi = "10.2298/ABS1304331C"
}

Ćujić, D., Bojić-Trbojević, Ž., Tošić, N., Pavlović, S.,& Vićovac, L.. (2013). Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(4), 1331-1337.
https://doi.org/10.2298/ABS1304331C

Ćujić D, Bojić-Trbojević Ž, Tošić N, Pavlović S, Vićovac L. Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro. in Archives of Biological Sciences. 2013;65(4):1331-1337.
doi:10.2298/ABS1304331C .

Ćujić, Danica, Bojić-Trbojević, Žanka, Tošić, Nataša, Pavlović, Sonja, Vićovac, Ljiljana, "Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro" in Archives of Biological Sciences, 65, no. 4 (2013):1331-1337,
https://doi.org/10.2298/ABS1304331C . .

TY  - JOUR
AU  - Kovacević, Tamara M.
AU  - Radojcić, Ljiljana
AU  - Tošić, Nataša
AU  - Pavlović, Sonja
AU  - Vicovac, Ljiljana M.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/702
AB  - Objective: Monoclonal antibody 26 (MAb 26) raised against tetanus toxoid has documented cross-reactivity with beta(2)-glycoprotein I. Passive introduction of this antibody in mice results in an antiphospholipid syndrome-like condition. We investigated the effects of MAb 26 on first trimester human trophoblast in vitro. Study design: Binding of MAb 26 to placental tissue trophoblast, isolated cytotrophoblast and HTR-8/SVneo cells was analyzed by immunohisto(cyto)chemistry. Possible effects on cell invasion in vitro were assessed by Matrigel assay. Effects on cell viability were assessed by MU test. A possibility that MAb 26 induces change in levels of effector molecules important for cell invasion was investigated. Integrin subunits alpha(1), alpha(5) and beta(1), and galectin-1, were analyzed by qPCR and Western blot. Metalloproteinases -2 and -9 were assessed by gelatin zymography. Results: Immunohisto(cyto)chemistry showed binding of MAb 26 to placental tissue trophoblast, isolated cytotrophoblast and HTR-8/SVneo cells. The antibody had a significant inhibitory effect on cell invasion by both isolated cytotrophoblast and HTR-8/SVneo. The antibody induced significant decrease in protein levels of metalloproteinases, integrin subunit alpha(1) and galectin-1. Cell viability was not affected. Conclusion: MAb 26 reduces trophoblast invasion in vitro through decreased levels of metalloproteinases-2 and -9, integrin alpha(1) and galectin-1.
PB  - Elsevier, Amsterdam
T2  - European Journal of Obstetrics & Gynecology and Reproductive Biology
T1  - Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro
EP  - 29
IS  - 1
SP  - 23
VL  - 171
DO  - 10.1016/j.ejogrb.2013.08.005
ER  - 

@article{
author = "Kovacević, Tamara M. and Radojcić, Ljiljana and Tošić, Nataša and Pavlović, Sonja and Vicovac, Ljiljana M.",
year = "2013",
abstract = "Objective: Monoclonal antibody 26 (MAb 26) raised against tetanus toxoid has documented cross-reactivity with beta(2)-glycoprotein I. Passive introduction of this antibody in mice results in an antiphospholipid syndrome-like condition. We investigated the effects of MAb 26 on first trimester human trophoblast in vitro. Study design: Binding of MAb 26 to placental tissue trophoblast, isolated cytotrophoblast and HTR-8/SVneo cells was analyzed by immunohisto(cyto)chemistry. Possible effects on cell invasion in vitro were assessed by Matrigel assay. Effects on cell viability were assessed by MU test. A possibility that MAb 26 induces change in levels of effector molecules important for cell invasion was investigated. Integrin subunits alpha(1), alpha(5) and beta(1), and galectin-1, were analyzed by qPCR and Western blot. Metalloproteinases -2 and -9 were assessed by gelatin zymography. Results: Immunohisto(cyto)chemistry showed binding of MAb 26 to placental tissue trophoblast, isolated cytotrophoblast and HTR-8/SVneo cells. The antibody had a significant inhibitory effect on cell invasion by both isolated cytotrophoblast and HTR-8/SVneo. The antibody induced significant decrease in protein levels of metalloproteinases, integrin subunit alpha(1) and galectin-1. Cell viability was not affected. Conclusion: MAb 26 reduces trophoblast invasion in vitro through decreased levels of metalloproteinases-2 and -9, integrin alpha(1) and galectin-1.",
publisher = "Elsevier, Amsterdam",
journal = "European Journal of Obstetrics & Gynecology and Reproductive Biology",
title = "Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro",
pages = "29-23",
number = "1",
volume = "171",
doi = "10.1016/j.ejogrb.2013.08.005"
}

Kovacević, T. M., Radojcić, L., Tošić, N., Pavlović, S.,& Vicovac, L. M.. (2013). Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro. in European Journal of Obstetrics & Gynecology and Reproductive Biology
Elsevier, Amsterdam., 171(1), 23-29.
https://doi.org/10.1016/j.ejogrb.2013.08.005

Kovacević TM, Radojcić L, Tošić N, Pavlović S, Vicovac LM. Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro. in European Journal of Obstetrics & Gynecology and Reproductive Biology. 2013;171(1):23-29.
doi:10.1016/j.ejogrb.2013.08.005 .

Kovacević, Tamara M., Radojcić, Ljiljana, Tošić, Nataša, Pavlović, Sonja, Vicovac, Ljiljana M., "Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro" in European Journal of Obstetrics & Gynecology and Reproductive Biology, 171, no. 1 (2013):23-29,
https://doi.org/10.1016/j.ejogrb.2013.08.005 . .