TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Cacev, Tamara
AU  - Aralica, Gorana
AU  - Hadzija, Marijana Popovic
AU  - Kapitanović, Sanja
AU  - Radojković, Dragica
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/884
AB  - This study was aimed at the analysis of mononudeotide repeats -462T(15) and -4T(12) in the SMAD4 gene promoter in sporadic colon adenocarcinoma tissue of Croatian patients. The analysis has included 60 pairs of samples of colon tumor and adjacent normal tissue. The number of thymidines in the tracts -462T(15) and -4T(12) of the SMAD4 gene promoter was determined by PCR with fluorescently labeled primers followed by the analysis of obtained DNA fragments by capillary electrophoresis. In the normal colon tissue two haplotypes were present: -462T(15)/4T(12) in 51 patients (85%) and -462T(16)/-4T(12) in 9 patients (15%). Among the cases with haplotype -462T(15)/-4T(12) detected in normal colon tissue, in 5 cases (8%) malignant tissue displayed different haplotypes: 462T(10)/-4T(10), -462T(12)/- 4T(12), 462T(13)/-4T(11), -462T(14)/-4T(10) and -462T(15)/-4T(11). Haplotype -462T(14)/-4T(10) was previously found to be associated with significantly decreased SMAD4 gene promoter activity in comparison to the wild type, while the other detected haplotypes remain to be functionally characterized. This study has shown that functionally relevant somatic alterations of the SMAD4 gene promoter are found in some colon cancer tumors. Although not as frequent in colon as in pancreatic cancer, they may be of significance for certain cases and their role in colon tumorigenesis should be investigated further.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental and Molecular Pathology
T1  - Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients
EP  - 135
IS  - 2
SP  - 133
VL  - 98
DO  - 10.1016/j.yexmp.2015.02.008
ER  - 

@article{
author = "Nikolić, Aleksandra and Cacev, Tamara and Aralica, Gorana and Hadzija, Marijana Popovic and Kapitanović, Sanja and Radojković, Dragica",
year = "2015",
abstract = "This study was aimed at the analysis of mononudeotide repeats -462T(15) and -4T(12) in the SMAD4 gene promoter in sporadic colon adenocarcinoma tissue of Croatian patients. The analysis has included 60 pairs of samples of colon tumor and adjacent normal tissue. The number of thymidines in the tracts -462T(15) and -4T(12) of the SMAD4 gene promoter was determined by PCR with fluorescently labeled primers followed by the analysis of obtained DNA fragments by capillary electrophoresis. In the normal colon tissue two haplotypes were present: -462T(15)/4T(12) in 51 patients (85%) and -462T(16)/-4T(12) in 9 patients (15%). Among the cases with haplotype -462T(15)/-4T(12) detected in normal colon tissue, in 5 cases (8%) malignant tissue displayed different haplotypes: 462T(10)/-4T(10), -462T(12)/- 4T(12), 462T(13)/-4T(11), -462T(14)/-4T(10) and -462T(15)/-4T(11). Haplotype -462T(14)/-4T(10) was previously found to be associated with significantly decreased SMAD4 gene promoter activity in comparison to the wild type, while the other detected haplotypes remain to be functionally characterized. This study has shown that functionally relevant somatic alterations of the SMAD4 gene promoter are found in some colon cancer tumors. Although not as frequent in colon as in pancreatic cancer, they may be of significance for certain cases and their role in colon tumorigenesis should be investigated further.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental and Molecular Pathology",
title = "Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients",
pages = "135-133",
number = "2",
volume = "98",
doi = "10.1016/j.yexmp.2015.02.008"
}

Nikolić, A., Cacev, T., Aralica, G., Hadzija, M. P., Kapitanović, S.,& Radojković, D.. (2015). Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients. in Experimental and Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 98(2), 133-135.
https://doi.org/10.1016/j.yexmp.2015.02.008

Nikolić A, Cacev T, Aralica G, Hadzija MP, Kapitanović S, Radojković D. Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients. in Experimental and Molecular Pathology. 2015;98(2):133-135.
doi:10.1016/j.yexmp.2015.02.008 .

Nikolić, Aleksandra, Cacev, Tamara, Aralica, Gorana, Hadzija, Marijana Popovic, Kapitanović, Sanja, Radojković, Dragica, "Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients" in Experimental and Molecular Pathology, 98, no. 2 (2015):133-135,
https://doi.org/10.1016/j.yexmp.2015.02.008 . .