@article{
author = "Jeremić, Ivica and Đurić, Olivera and Nikolić, Milos and Vlajnić, Marina and Nikolić, Aleksandra and Radojković, Dragica and Bonaci-Nikolić, Branka",
year = "2019",
abstract = "Neutrophil extracellular traps (NETs) are the main source of autoantigens in systemic lupus erythematosus (SLE). The aim of this study was to evaluate the clinical importance of NETs-associated markers in SLE. We compared NETs-associated markers in SLE patients (n = 111) with healthy controls (n = 50). Moreover, in 35 patients with drug-naive SLE (n = 35), we investigated correlation between NETs-associated markers [DNase I concentration, myeloperoxidase (MPO) activity, anti-MPO antibodies, cell-free DNA (cfDNA), NETolytic activity] with serological parameters [anti-dsDNA antibodies, C3, C4 and B-cell activating factor (BAFF) levels] and disease activity measured by modified SLE Disease Activity Index (M-SLEDAI-2K). In comparison with healthy controls, SLE patients had higher cfDNA, MPO activity, anti-MPO antibodies (p lt 0.001), BAFF and DNase I concentration (p lt 0.01). Contrary, NETolytic activity was lower in SLE patients (p lt 0.05), despite higher concentration of DNase I. MPO activity and cfDNA levels showed correlation with DNase I concentration (p lt 0.001, p lt 0.01, respectively). BAFF levels correlated with cfDNA, DNase I concentration and MPO activity (p lt 0.05). Anti-dsDNA antibodies showed correlation with MPO activity (p lt 0.01), cfDNA and BAFF levels (p lt 0.001). Anti-dsDNA and C3 levels were independent predictors of M-SLEDAI-2K in multivariate analysis (p lt 0.01). We demonstrated that sera of SLE patients have decreased NETolytic activity, leading to increased levels of various NETs-associated markers, which correlate with anti-dsDNA antibodies in drug-naive SLE. We showed that BAFF participates in a complex relationship between NETosis and anti-dsDNA antibodies production. These findings have important implications for a better understanding of SLE pathogenesis and development of therapy that inhibits NETs persistence and disease progression.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Rheumatology International",
title = "Neutrophil extracellular traps-associated markers are elevated in patients with systemic lupus erythematosus",
pages = "1857-1849",
number = "11",
volume = "39",
doi = "10.1007/s00296-019-04426-1"
}