TY  - JOUR
AU  - Banović Đeri, Bojana
AU  - Bozić, Manja
AU  - Dudić, Dragana
AU  - Vicić, Ivan
AU  - Milivojević, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Samardžić, Jelena
AU  - Vancetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1576
AB  - One of the strategies for overcoming global climate change threatening to decrease maize yield is early sowing. To contribute to the development of cold-tolerant hybrids this research focused on the genetic background's comparative analysis in maize inbreds with good combining ability. Leaf whole-transcriptome sequencing of 46 maize genotypes revealed 77 differentially expressed genes (DEGs) between Lancaster and other heterotic groups (i.e. BSSS, Iowa dent, Ohio), referred to as non-Lancaster group, under optimal growing conditions. Cold test of the subset of four Lancaster and four non-Lancaster lines showed that the former were cold sensitive and the latter cold tolerant. Cold-induced expression analysis of seven DEGs in eight lines revealed different expression regulation dependent on the duration of cold exposure and genetic background for six out of seven analysed genes-chloroplast ATP-sulphurylase, photosystem II cytochrome b559 alpha subunit, CIPK serine-threonine protein kinase 15, glutamyl-tRNA reductase, photosystem II reaction centre protein I and Calvin cycle CP12-chloroplastic-like encoding genes. The results imply that differently regulated basic processes between Lancaster and non-Lancaster maize group involve, at least, photosynthesis and sulphate assimilation, contributing to their different cold response and different adaptation to low temperatures.
PB  - Wiley, Hoboken
T2  - Journal of Agronomy and Crop Science
T1  - Leaf transcriptome analysis of Lancaster versus other heterotic groups' maize inbred lines revealed different regulation of cold-responsive genes
EP  - 509
IS  - 4
SP  - 497
VL  - 208
DO  - 10.1111/jac.12529
ER  - 

@article{
author = "Banović Đeri, Bojana and Bozić, Manja and Dudić, Dragana and Vicić, Ivan and Milivojević, Marija and Ignjatović-Micić, Dragana and Samardžić, Jelena and Vancetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2022",
abstract = "One of the strategies for overcoming global climate change threatening to decrease maize yield is early sowing. To contribute to the development of cold-tolerant hybrids this research focused on the genetic background's comparative analysis in maize inbreds with good combining ability. Leaf whole-transcriptome sequencing of 46 maize genotypes revealed 77 differentially expressed genes (DEGs) between Lancaster and other heterotic groups (i.e. BSSS, Iowa dent, Ohio), referred to as non-Lancaster group, under optimal growing conditions. Cold test of the subset of four Lancaster and four non-Lancaster lines showed that the former were cold sensitive and the latter cold tolerant. Cold-induced expression analysis of seven DEGs in eight lines revealed different expression regulation dependent on the duration of cold exposure and genetic background for six out of seven analysed genes-chloroplast ATP-sulphurylase, photosystem II cytochrome b559 alpha subunit, CIPK serine-threonine protein kinase 15, glutamyl-tRNA reductase, photosystem II reaction centre protein I and Calvin cycle CP12-chloroplastic-like encoding genes. The results imply that differently regulated basic processes between Lancaster and non-Lancaster maize group involve, at least, photosynthesis and sulphate assimilation, contributing to their different cold response and different adaptation to low temperatures.",
publisher = "Wiley, Hoboken",
journal = "Journal of Agronomy and Crop Science",
title = "Leaf transcriptome analysis of Lancaster versus other heterotic groups' maize inbred lines revealed different regulation of cold-responsive genes",
pages = "509-497",
number = "4",
volume = "208",
doi = "10.1111/jac.12529"
}

Banović Đeri, B., Bozić, M., Dudić, D., Vicić, I., Milivojević, M., Ignjatović-Micić, D., Samardžić, J., Vancetović, J., Delić, N.,& Nikolić, A.. (2022). Leaf transcriptome analysis of Lancaster versus other heterotic groups' maize inbred lines revealed different regulation of cold-responsive genes. in Journal of Agronomy and Crop Science
Wiley, Hoboken., 208(4), 497-509.
https://doi.org/10.1111/jac.12529

Banović Đeri B, Bozić M, Dudić D, Vicić I, Milivojević M, Ignjatović-Micić D, Samardžić J, Vancetović J, Delić N, Nikolić A. Leaf transcriptome analysis of Lancaster versus other heterotic groups' maize inbred lines revealed different regulation of cold-responsive genes. in Journal of Agronomy and Crop Science. 2022;208(4):497-509.
doi:10.1111/jac.12529 .

Banović Đeri, Bojana, Bozić, Manja, Dudić, Dragana, Vicić, Ivan, Milivojević, Marija, Ignjatović-Micić, Dragana, Samardžić, Jelena, Vancetović, Jelena, Delić, Nenad, Nikolić, Ana, "Leaf transcriptome analysis of Lancaster versus other heterotic groups' maize inbred lines revealed different regulation of cold-responsive genes" in Journal of Agronomy and Crop Science, 208, no. 4 (2022):497-509,
https://doi.org/10.1111/jac.12529 . .

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Ana
AU  - Nikolić, Aleksandra
AU  - Kostadinović, Marija
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1016
AB  - Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples - with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of -IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - DNA and biochemical analysis of a potential opaque2 maize population
EP  - 61
IS  - 1
SP  - 51
VL  - 49
DO  - 10.2298/GENSR1701051I
ER  - 

@article{
author = "Ignjatović-Micić, Dragana and Nikolić, Ana and Nikolić, Aleksandra and Kostadinović, Marija",
year = "2017",
abstract = "Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples - with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of -IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "DNA and biochemical analysis of a potential opaque2 maize population",
pages = "61-51",
number = "1",
volume = "49",
doi = "10.2298/GENSR1701051I"
}

Ignjatović-Micić, D., Nikolić, A., Nikolić, A.,& Kostadinović, M.. (2017). DNA and biochemical analysis of a potential opaque2 maize population. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 49(1), 51-61.
https://doi.org/10.2298/GENSR1701051I

Ignjatović-Micić D, Nikolić A, Nikolić A, Kostadinović M. DNA and biochemical analysis of a potential opaque2 maize population. in Genetika-Belgrade. 2017;49(1):51-61.
doi:10.2298/GENSR1701051I .

Ignjatović-Micić, Dragana, Nikolić, Ana, Nikolić, Aleksandra, Kostadinović, Marija, "DNA and biochemical analysis of a potential opaque2 maize population" in Genetika-Belgrade, 49, no. 1 (2017):51-61,
https://doi.org/10.2298/GENSR1701051I . .