TY  - JOUR
AU  - Samardzija, Dragana
AU  - Pogrmić-Majkić, Kristina
AU  - Fa, Svetlana
AU  - Stanić, Bojana
AU  - Jasnić, Jovana
AU  - Andrić, Nebojša
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1193
AB  - Bisphenol A (BPA) is an endocrine disruptor used in a variety of consumer products. Exposure to BPA leads to alterations in steroidogenesis of ovarian granulosa cells. Here, we analyzed the mechanism by which BPA alters progesterone biosynthesis in immature rat granulosa cells. BPA increased expression of steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme and 3 beta-hydroxysteroid dehydrogenase in granulosa cells; however, BPA prevented the basal and the FSH-induced progesterone production. BPA caused sequestration of cholesterol to the perinuclear area, as evident by the Filipin staining. BPA decreased mRNA expression of ATP binding cassette transporter-A1 (Abca1) and increased level of sterol regulatory element binding protein 1. Addition of exogenous cell-permeable cholesterol restored the effect of BPA on Abca1 and Star mRNA expression and partially reversed BPA's effect on progesterone production. These results indicate that exposure to BPA disrupts cholesterol homeostasis leading to decreased progesterone production in immature rat granulosa cells.
PB  - Elsevier Ireland Ltd, Clare
T2  - Molecular and Cellular Endocrinology
T1  - Bisphenol A decreases progesterone synthesis by disrupting cholesterol homeostasis in rat granulosa cells
EP  - 63
IS  - C
SP  - 55
VL  - 461
DO  - 10.1016/j.mce.2017.08.013
ER  - 

@article{
author = "Samardzija, Dragana and Pogrmić-Majkić, Kristina and Fa, Svetlana and Stanić, Bojana and Jasnić, Jovana and Andrić, Nebojša",
year = "2018",
abstract = "Bisphenol A (BPA) is an endocrine disruptor used in a variety of consumer products. Exposure to BPA leads to alterations in steroidogenesis of ovarian granulosa cells. Here, we analyzed the mechanism by which BPA alters progesterone biosynthesis in immature rat granulosa cells. BPA increased expression of steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme and 3 beta-hydroxysteroid dehydrogenase in granulosa cells; however, BPA prevented the basal and the FSH-induced progesterone production. BPA caused sequestration of cholesterol to the perinuclear area, as evident by the Filipin staining. BPA decreased mRNA expression of ATP binding cassette transporter-A1 (Abca1) and increased level of sterol regulatory element binding protein 1. Addition of exogenous cell-permeable cholesterol restored the effect of BPA on Abca1 and Star mRNA expression and partially reversed BPA's effect on progesterone production. These results indicate that exposure to BPA disrupts cholesterol homeostasis leading to decreased progesterone production in immature rat granulosa cells.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Molecular and Cellular Endocrinology",
title = "Bisphenol A decreases progesterone synthesis by disrupting cholesterol homeostasis in rat granulosa cells",
pages = "63-55",
number = "C",
volume = "461",
doi = "10.1016/j.mce.2017.08.013"
}

Samardzija, D., Pogrmić-Majkić, K., Fa, S., Stanić, B., Jasnić, J.,& Andrić, N.. (2018). Bisphenol A decreases progesterone synthesis by disrupting cholesterol homeostasis in rat granulosa cells. in Molecular and Cellular Endocrinology
Elsevier Ireland Ltd, Clare., 461(C), 55-63.
https://doi.org/10.1016/j.mce.2017.08.013

Samardzija D, Pogrmić-Majkić K, Fa S, Stanić B, Jasnić J, Andrić N. Bisphenol A decreases progesterone synthesis by disrupting cholesterol homeostasis in rat granulosa cells. in Molecular and Cellular Endocrinology. 2018;461(C):55-63.
doi:10.1016/j.mce.2017.08.013 .

Samardzija, Dragana, Pogrmić-Majkić, Kristina, Fa, Svetlana, Stanić, Bojana, Jasnić, Jovana, Andrić, Nebojša, "Bisphenol A decreases progesterone synthesis by disrupting cholesterol homeostasis in rat granulosa cells" in Molecular and Cellular Endocrinology, 461, no. C (2018):55-63,
https://doi.org/10.1016/j.mce.2017.08.013 . .