TY  - JOUR
AU  - Lukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Terzić-Vidojević, Amarela
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Ljubobratović, Uros
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1504
AB  - This research aimed to test whether coating with Lactobacillus paracasei subsp. paracasei BGHN14 homogenate may increase soluble protein and amino acid amount on larval fish feed surface. Total amino acid and protein, as well as taste stimulating and growth promoting amino acid amounts were analyzed in coated feed samples. Results indicated that coating with BGHN14 homogenate increased the amount of soluble proteins and free Glycine on feed surface, as well as the availability of protein-bound (hydroxy-)Proline. This lab-scale research provides the basis for use of lactobacilli as resource efficient source of soluble nutrients for animal feedstuffs.
PB  - Central Fisheries Research Inst, Trabzon
T2  - Turkish Journal of Fisheries and Aquatic Sciences
T1  - Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate
EP  - 573
IS  - 11
SP  - 569
VL  - 21
DO  - 10.4194/1303-2712-v21_11_05
ER  - 

@article{
author = "Lukić, Jovanka and Stanisavljević, Nemanja and Vukotić, Goran and Terzić-Vidojević, Amarela and Begović, Jelena and Golić, Nataša and Ljubobratović, Uros",
year = "2021",
abstract = "This research aimed to test whether coating with Lactobacillus paracasei subsp. paracasei BGHN14 homogenate may increase soluble protein and amino acid amount on larval fish feed surface. Total amino acid and protein, as well as taste stimulating and growth promoting amino acid amounts were analyzed in coated feed samples. Results indicated that coating with BGHN14 homogenate increased the amount of soluble proteins and free Glycine on feed surface, as well as the availability of protein-bound (hydroxy-)Proline. This lab-scale research provides the basis for use of lactobacilli as resource efficient source of soluble nutrients for animal feedstuffs.",
publisher = "Central Fisheries Research Inst, Trabzon",
journal = "Turkish Journal of Fisheries and Aquatic Sciences",
title = "Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate",
pages = "573-569",
number = "11",
volume = "21",
doi = "10.4194/1303-2712-v21_11_05"
}

Lukić, J., Stanisavljević, N., Vukotić, G., Terzić-Vidojević, A., Begović, J., Golić, N.,& Ljubobratović, U.. (2021). Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate. in Turkish Journal of Fisheries and Aquatic Sciences
Central Fisheries Research Inst, Trabzon., 21(11), 569-573.
https://doi.org/10.4194/1303-2712-v21_11_05

Lukić J, Stanisavljević N, Vukotić G, Terzić-Vidojević A, Begović J, Golić N, Ljubobratović U. Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate. in Turkish Journal of Fisheries and Aquatic Sciences. 2021;21(11):569-573.
doi:10.4194/1303-2712-v21_11_05 .

Lukić, Jovanka, Stanisavljević, Nemanja, Vukotić, Goran, Terzić-Vidojević, Amarela, Begović, Jelena, Golić, Nataša, Ljubobratović, Uros, "Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate" in Turkish Journal of Fisheries and Aquatic Sciences, 21, no. 11 (2021):569-573,
https://doi.org/10.4194/1303-2712-v21_11_05 . .

TY  - JOUR
AU  - Ljubobratović, Uros
AU  - Fazekas, Georgina
AU  - Koljukaj, Alan
AU  - Ristović, Tijana
AU  - Vass, Vivien
AU  - Ardo, Laszlo
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Pesić, Mirjana
AU  - Milincić, Danijel
AU  - Kostić, Aleksandar
AU  - Lukić, Jovanka
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1448
AB  - This study evaluated whether inert feed enriched with Lb. paracasei subsp. paracasei BGHN14 may be used as a weaning diet for first feeding pike-perch larvae. Three experimental groups were weaned from the start of exogenous feeding: two groups were given inert feed enriched with BGHN14 either via 12 h incubation with live BGHN14 cells or via coating with homogenized BGHN14 cells and one group was supplemented non-enriched inert feed. In all three groups Artemia was co-fed with inert feed during weaning. Control group larvae were fed Artemia exclusively during the treatment period. Treatment lasted fourteen days, starting from the 6th day post-hatch (DPH). Larval sampling was performed on the 20th DPH for gene expression and enzyme activity analysis. Larvae were also sampled on the 32nd DPH for morphometric and body composition analysis. Our results showed that weaning of first feeding pike-perch larvae was associated with an increase of fish condition (0.72 +/- 0.12-0.77 +/- 0.11 versus 0.67 +/- 0.11 in controls), but it suppressed skeleton development, according to Col1 mRNA expression (1 +/- 0.51-1.06 +/- 0.36 versus 2.07 +/- 0.53 in controls) and reduced fat deposition (1.25 +/- 0.23-1.49 +/- 0.33 versus 1.84 +/- 0.31% in controls). This presumably reflected lower availability of soluble proteins in microdiet as opposed to live food, along with high leaching rate of amino acids from solid feed particles, as reported in our previous studies. However, skeleton differentiation was not impaired in group weaned on BGHN14 homogenate coated feed (Col1 mRNA expression: 2.68 +/- 0.72), which was enriched in skeleton building and taste stimulating amino acids. These larvae were also presented with substantially higher length (15.28 +/- 2.55 versus 13.93 +/- 2.31 mm in controls) and weight (26.56 +/- 13.83 versus 21.03 +/- 11.25 mg in controls), which correlated with lower trypsin activity (1.06 +/- 0.13 versus 1.43 +/- 0.26 mU/mg of proteins in controls) and an increase of PLA2 to trypsin activity ratio (453.12 +/- 109.36 versus 264.84 +/- 69.03 in controls). Present study suggests that weaning of first feeding pike-perch larvae using BGHN14 homogenate coated microdiet supports skeleton development and improves fish growth.
PB  - Elsevier, Amsterdam
T2  - Aquaculture
T1  - Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding
VL  - 542
DO  - 10.1016/j.aquaculture.2021.736901
ER  - 

@article{
author = "Ljubobratović, Uros and Fazekas, Georgina and Koljukaj, Alan and Ristović, Tijana and Vass, Vivien and Ardo, Laszlo and Stanisavljević, Nemanja and Vukotić, Goran and Pesić, Mirjana and Milincić, Danijel and Kostić, Aleksandar and Lukić, Jovanka",
year = "2021",
abstract = "This study evaluated whether inert feed enriched with Lb. paracasei subsp. paracasei BGHN14 may be used as a weaning diet for first feeding pike-perch larvae. Three experimental groups were weaned from the start of exogenous feeding: two groups were given inert feed enriched with BGHN14 either via 12 h incubation with live BGHN14 cells or via coating with homogenized BGHN14 cells and one group was supplemented non-enriched inert feed. In all three groups Artemia was co-fed with inert feed during weaning. Control group larvae were fed Artemia exclusively during the treatment period. Treatment lasted fourteen days, starting from the 6th day post-hatch (DPH). Larval sampling was performed on the 20th DPH for gene expression and enzyme activity analysis. Larvae were also sampled on the 32nd DPH for morphometric and body composition analysis. Our results showed that weaning of first feeding pike-perch larvae was associated with an increase of fish condition (0.72 +/- 0.12-0.77 +/- 0.11 versus 0.67 +/- 0.11 in controls), but it suppressed skeleton development, according to Col1 mRNA expression (1 +/- 0.51-1.06 +/- 0.36 versus 2.07 +/- 0.53 in controls) and reduced fat deposition (1.25 +/- 0.23-1.49 +/- 0.33 versus 1.84 +/- 0.31% in controls). This presumably reflected lower availability of soluble proteins in microdiet as opposed to live food, along with high leaching rate of amino acids from solid feed particles, as reported in our previous studies. However, skeleton differentiation was not impaired in group weaned on BGHN14 homogenate coated feed (Col1 mRNA expression: 2.68 +/- 0.72), which was enriched in skeleton building and taste stimulating amino acids. These larvae were also presented with substantially higher length (15.28 +/- 2.55 versus 13.93 +/- 2.31 mm in controls) and weight (26.56 +/- 13.83 versus 21.03 +/- 11.25 mg in controls), which correlated with lower trypsin activity (1.06 +/- 0.13 versus 1.43 +/- 0.26 mU/mg of proteins in controls) and an increase of PLA2 to trypsin activity ratio (453.12 +/- 109.36 versus 264.84 +/- 69.03 in controls). Present study suggests that weaning of first feeding pike-perch larvae using BGHN14 homogenate coated microdiet supports skeleton development and improves fish growth.",
publisher = "Elsevier, Amsterdam",
journal = "Aquaculture",
title = "Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding",
volume = "542",
doi = "10.1016/j.aquaculture.2021.736901"
}

Ljubobratović, U., Fazekas, G., Koljukaj, A., Ristović, T., Vass, V., Ardo, L., Stanisavljević, N., Vukotić, G., Pesić, M., Milincić, D., Kostić, A.,& Lukić, J.. (2021). Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding. in Aquaculture
Elsevier, Amsterdam., 542.
https://doi.org/10.1016/j.aquaculture.2021.736901

Ljubobratović U, Fazekas G, Koljukaj A, Ristović T, Vass V, Ardo L, Stanisavljević N, Vukotić G, Pesić M, Milincić D, Kostić A, Lukić J. Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding. in Aquaculture. 2021;542.
doi:10.1016/j.aquaculture.2021.736901 .

Ljubobratović, Uros, Fazekas, Georgina, Koljukaj, Alan, Ristović, Tijana, Vass, Vivien, Ardo, Laszlo, Stanisavljević, Nemanja, Vukotić, Goran, Pesić, Mirjana, Milincić, Danijel, Kostić, Aleksandar, Lukić, Jovanka, "Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding" in Aquaculture, 542 (2021),
https://doi.org/10.1016/j.aquaculture.2021.736901 . .

TY  - JOUR
AU  - Ljubobratović, Uros
AU  - Kosanović, Dejana
AU  - Demeny, Ferenc Zoltan
AU  - Krajcsovics, Adrienn
AU  - Vukotić, Goran
AU  - Stanisavljević, Nemanja
AU  - Golić, Nataša
AU  - Jeney, Galina
AU  - Lukić, Jovanka
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1402
AB  - Weaning to inert diet in intensively reared pike-perch larvae is confronted with significant fish loss, which prevents successful commercialization of pike-perch production. Achievement of satisfactory feed quality and effective assimilation of nutrients by larval fish is the major challenge in larval production process. Aim of this study was to evaluate whether treatment of live and inert feed with lactobacilli could alleviate growth retardation associated with early weaning of pike-perch reared in recirculating system. Weaning started on 18th day post-hatching (DPH) either as sudden weaning (SW) or by co-administration of Anemia for six days (gradual weaning, GW). Prior to administration to fish, Anemia was treated with Lactobacillus salivarius BGHO1/Lb. reuteri BGGO6-55, while inert feed was treated with Lb. paracasei subsp. paracasei BGHN14/Lb. rhamnosus BGT10. Treatment with lactobacilli slightly raised neutral lipid level in Anemia nauplii, but significantly reduced their content in dry feed. Fish were sampled on the 24th DPH. Survival, morphometric indices, skeleton differentiation, digestive enzyme activity and opportunistic pathogenic bacteria level were assessed in whole fish specimens. GW fish were presented with better survival, body growth and phospholipase A2 (PLA2) activity. Alongside, Vibrio spp. growth was suppressed in these fish and skeleton development was improved, according to Alizarin Red staining and ColIA1/Sparc mRNA expression data. Lactobacilli application in GW fish correlated with an increase of survival, condition factor and growth rate, according to trypsin and chymotrypsin activities, indicating better utilization of dietary proteins for muscle building. In SW fish, lactobacilli elevated chymotrypsin activity, PLA2 to lipase activity ratio and improved survival and ossification, as evident from Alizarin Red staining and ColIA1/Sparc mRNA expression. This indicated improved fatty acid absorption and control of metamorphosis process. Furthermore, lactobacilli suppressed Vibrio spp. growth in SW fish. Aside from demonstrating the ability of lactobacilli to aid weaning in pike-perch larvae, this study indicated that different types of food treatment may direct fish growth in a predictable manner, allowing further cost-effective improvements of larval pike-perch rearing in intensive system.
PB  - Elsevier, Amsterdam
T2  - Aquaculture
T1  - The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae
VL  - 516
DO  - 10.1016/j.aquaculture.2019.734608
ER  - 

@article{
author = "Ljubobratović, Uros and Kosanović, Dejana and Demeny, Ferenc Zoltan and Krajcsovics, Adrienn and Vukotić, Goran and Stanisavljević, Nemanja and Golić, Nataša and Jeney, Galina and Lukić, Jovanka",
year = "2020",
abstract = "Weaning to inert diet in intensively reared pike-perch larvae is confronted with significant fish loss, which prevents successful commercialization of pike-perch production. Achievement of satisfactory feed quality and effective assimilation of nutrients by larval fish is the major challenge in larval production process. Aim of this study was to evaluate whether treatment of live and inert feed with lactobacilli could alleviate growth retardation associated with early weaning of pike-perch reared in recirculating system. Weaning started on 18th day post-hatching (DPH) either as sudden weaning (SW) or by co-administration of Anemia for six days (gradual weaning, GW). Prior to administration to fish, Anemia was treated with Lactobacillus salivarius BGHO1/Lb. reuteri BGGO6-55, while inert feed was treated with Lb. paracasei subsp. paracasei BGHN14/Lb. rhamnosus BGT10. Treatment with lactobacilli slightly raised neutral lipid level in Anemia nauplii, but significantly reduced their content in dry feed. Fish were sampled on the 24th DPH. Survival, morphometric indices, skeleton differentiation, digestive enzyme activity and opportunistic pathogenic bacteria level were assessed in whole fish specimens. GW fish were presented with better survival, body growth and phospholipase A2 (PLA2) activity. Alongside, Vibrio spp. growth was suppressed in these fish and skeleton development was improved, according to Alizarin Red staining and ColIA1/Sparc mRNA expression data. Lactobacilli application in GW fish correlated with an increase of survival, condition factor and growth rate, according to trypsin and chymotrypsin activities, indicating better utilization of dietary proteins for muscle building. In SW fish, lactobacilli elevated chymotrypsin activity, PLA2 to lipase activity ratio and improved survival and ossification, as evident from Alizarin Red staining and ColIA1/Sparc mRNA expression. This indicated improved fatty acid absorption and control of metamorphosis process. Furthermore, lactobacilli suppressed Vibrio spp. growth in SW fish. Aside from demonstrating the ability of lactobacilli to aid weaning in pike-perch larvae, this study indicated that different types of food treatment may direct fish growth in a predictable manner, allowing further cost-effective improvements of larval pike-perch rearing in intensive system.",
publisher = "Elsevier, Amsterdam",
journal = "Aquaculture",
title = "The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae",
volume = "516",
doi = "10.1016/j.aquaculture.2019.734608"
}

Ljubobratović, U., Kosanović, D., Demeny, F. Z., Krajcsovics, A., Vukotić, G., Stanisavljević, N., Golić, N., Jeney, G.,& Lukić, J.. (2020). The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae. in Aquaculture
Elsevier, Amsterdam., 516.
https://doi.org/10.1016/j.aquaculture.2019.734608

Ljubobratović U, Kosanović D, Demeny FZ, Krajcsovics A, Vukotić G, Stanisavljević N, Golić N, Jeney G, Lukić J. The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae. in Aquaculture. 2020;516.
doi:10.1016/j.aquaculture.2019.734608 .

Ljubobratović, Uros, Kosanović, Dejana, Demeny, Ferenc Zoltan, Krajcsovics, Adrienn, Vukotić, Goran, Stanisavljević, Nemanja, Golić, Nataša, Jeney, Galina, Lukić, Jovanka, "The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae" in Aquaculture, 516 (2020),
https://doi.org/10.1016/j.aquaculture.2019.734608 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Vukotić, Goran
AU  - Stanisavljević, Nemanja
AU  - Kosanović, Dejana
AU  - Molnar, Zsuzsanna
AU  - Begović, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Jeney, Galina
AU  - Ljubobratović, Uros
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1207
AB  - The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracaseisubsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed
IS  - 7
VL  - 14
DO  - 10.1371/journal.pone.0219558
ER  - 

@article{
author = "Lukić, Jovanka and Vukotić, Goran and Stanisavljević, Nemanja and Kosanović, Dejana and Molnar, Zsuzsanna and Begović, Jelena and Terzić-Vidojević, Amarela and Jeney, Galina and Ljubobratović, Uros",
year = "2019",
abstract = "The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracaseisubsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed",
number = "7",
volume = "14",
doi = "10.1371/journal.pone.0219558"
}

Lukić, J., Vukotić, G., Stanisavljević, N., Kosanović, D., Molnar, Z., Begović, J., Terzić-Vidojević, A., Jeney, G.,& Ljubobratović, U.. (2019). Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed. in PLoS One
Public Library Science, San Francisco., 14(7).
https://doi.org/10.1371/journal.pone.0219558

Lukić J, Vukotić G, Stanisavljević N, Kosanović D, Molnar Z, Begović J, Terzić-Vidojević A, Jeney G, Ljubobratović U. Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed. in PLoS One. 2019;14(7).
doi:10.1371/journal.pone.0219558 .

Lukić, Jovanka, Vukotić, Goran, Stanisavljević, Nemanja, Kosanović, Dejana, Molnar, Zsuzsanna, Begović, Jelena, Terzić-Vidojević, Amarela, Jeney, Galina, Ljubobratović, Uros, "Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed" in PLoS One, 14, no. 7 (2019),
https://doi.org/10.1371/journal.pone.0219558 . .